Publications

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antiprotozoal, antimycobacterial, and anti-inflammatory evaluation of cnidoscolus chayamansa (mc vaugh) extract and the isolated compounds.cnidoscolus chayamansa is a medicinal and edible plant known as chaya, is commonly used as an anti-inflammatory, antiprotozoal, antibacterial agent and as a remedy for respiratory illness, gastrointestinal disorders, and vaginal infections related with the inflammation process. in this paper, we describe the plant's phytochemical analysis and biological activities (antimycobacterial, antibacterial, antiprotozoal, and anti-inflammatory properties) of the chcl3:meoh (1:1) leaves extract and isolat ...201728222400
antiprotozoal constituents from annona cherimola miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery.annona cherimola miller (annonaceae) is a medicinal plant frequently recommended in mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.201728216899
prevalence and risk factors for intestinal parasitic infection in schoolchildren in battambang, cambodia.most intestinal parasitic infections (ipis), commonly endemic in tropical resource-poor developing countries, are neglected tropical diseases. parasitic infections and malnutrition are most commonly found in children. we determined the prevalence of ipis and the risk factors in battambang province, northwestern cambodia, from august to september 2015. this study collected 308 valid questionnaires and specimens from dontri (173, 56.2%) and kon kaêk (135, 43.8%) primary schools. all stool samples ...201728070012
prevalence of intestinal parasites among food handlers in western iran.parasitic infection is one of the problems that affect human health, especially in developing countries. in this study, all of the fast food shops, restaurants, and roast meat outlets of khorramabad (western iran) and all the staff employed by them, some 210 people, were selected through a census and their stools were examined for the presence of parasites. the parasitological tests of direct wet-mount, lugol's iodine staining, formaldehyde-ether sedimentation and trichrome staining techniques w ...201724626411
possible hematological changes associated with acute gastroenteritis among kindergarten children in gaza.gastroenteritis is considered one of the leading causes of morbidity and mortality in children especially in developing countries. it is a major childhood problem in gaza and one of the most common etiologic agents of iron deficiency anemia (ida).201726229719
comparative cell biology and evolution of annexins in diplomonads.annexins are multifunctional, calcium-binding proteins found in organisms across all kingdoms. most studies of annexins from single-celled eukaryotes have focused on the alpha-giardins, proteins assigned to the group e annexins, expressed by the diplomonad giardia intestinalis. we have characterized the annexin gene family in another diplomonad parasite, spironucleus salmonicida, by phylogenetic and experimental approaches. we constructed a comprehensive phylogeny of the diplomonad group e annex ...201727303715
characterization of telomeres and telomerase from the single-celled eukaryote giardia intestinalis.the ends of linear chromosomes, telomeres, are most commonly maintained by the enzyme telomerase. our study presents the characteristics of telomeres and telomerase from the single-celled parasitic eukaryote giardia intestinalis. using fluorescence in situ hybridization, we localized telomeres during all stages of the trophozoite cell cycle and demonstrated differences in the observed number of telomeric foci, indicating telomere clustering. the length of giardia telomeres was determined in diff ...201727638151
interactions between giardia duodenalis sm proteins and their association with spliceosomal snrnas.giardia duodenalis is a parasite that colonises the intestines of humans and other vertebrates, causing diarrhoea and poor nutrient absorption. g. duodenalis is sometimes considered an early diverging eukaryote, and its genome exhibits simplified molecular machinery for many cellular processes, which makes it an interesting model to study. the spliceosome, one of the most complex molecular machines in the eukaryotic cell, is responsible for intron excision and exon splicing. just over a decade a ...201727864674
synchronization of pathogenic protozoans.protozoans are single-celled eukaryotes and many of the best-studied protozoans are parasitic to humans (e.g., plasmodium falciparum causing malaria and trypanosoma brucei causing sleeping sickness). these organisms are distantly related to humans but with retained eukaryotic type of cellular processes, making them good model systems for studies of the evolution of basic processes like the cell cycle. giardia intestinalis causes 250 million cases of diarrhea yearly and is one of the earliest div ...201727815907
strong genetic structure revealed by multilocus patterns of variation in giardia duodenalis isolates of patients from galicia (nw-iberian peninsula).we report a survey of genetic variation at three coding loci in giardia duodenalis of assemblages a and b obtained from stool samples of patients from santiago de compostela (galicia, nw-iberian peninsula). the mean pooled synonymous diversity for assemblage a was nearly five times lower than for assemblage b (0.77%±0.30% and 4.14%±1.65%, respectively). synonymous variation in both assemblages was in mutation-drift equilibrium and an excess of low-frequency nonsynonymous variants suggested the a ...201727993728
nested pcr targeting intergenic spacer (igs) in genotyping of giardia duodenalis isolated from symptomatic and asymptomatic infected egyptian school children.distinct sequences of giardia duodenalis assemblages raised the hypothesis that certain assemblages may contribute to its clinical outcome. however, sequences analysis is time consuming, expensive, and needs many manual operations. nested pcr targeting intergenic spacer (igs) region was applied successfully to genotype g. duodenalis. this study aimed to identify the prevalence of g. duodenalis assemblages among giardiasis school children and its relation to the presence of symptoms using nested ...201727975120
prevalence of giardia duodenalis assemblages and sub-assemblages in symptomatic patients from damascus city and its suburbs.giardia duodenalis is one of the most important human enteric parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. however, there is limited information on the prevalent genetic variability of g. duodenalis in syria. this study aimed to evaluate the predominance of g. duodenalis assemblages/sub-assemblages causing humans infection in the city of damascus and its suburbs. 40 symptomatic giardiasis patients were recruited in this study. fecal samples were g ...201727919804
multilocus sequence analysis of giardia spp. isolated from patients with diarrhea in austria.giardia duodenalis is a protozoan parasite causing intestinal infections in a wide range of mammals. two distinct assemblages, a and b, infect humans predominantly; however, both are believed to be generally zoonotic. giardia strains associated with infections in austria have not been investigated at the molecular level. in this study, 65 human stool samples microscopically positive for giardia spp. were subjected to dna isolation and nested pcr targeting fragments of the glutamate dehydrogenase ...201727885465
intestinal parasites of children and adults in a remote aboriginal community of the northern territory, australia, 1994-1996.parasitic infections can adversely impact health, nutritional status and educational attainment. this study investigated hookworm and other intestinal parasites in an aboriginal community in australia from 1994 to 1996.201725960921
blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of colombia.blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (st), some of which are exclusively found in non-human hosts. the aim of this study was to determine the distribution of blastocystis sts/18s alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic chil ...201728222192
diagnosis of intestinal parasites in a rural community of venezuela: advantages and disadvantages of using microscopy or rt-pcr.a cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time pcr (rt-pcr) for 14 intestinal parasites in a venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. a total of 228 participants were included in this study. a multiplex rt-pcr was used for the detection of dientamoeba fragilis, giardia intestinalis, cryptosporidium sp. and ...201728007484
the effects of phosphanegold(i) thiolates on the biological properties of acanthamoeba castellanii belonging to the t4 genotype.gold compounds have shown promise in the treatment of non-communicable diseases such as rheumatoid arthritis and cancer, and are considered of value as anti-microbial agents against gram-negative and gram-positive bacteria, and have anti-parasitic properties against schistosoma mansoni, trypanosoma brucei, plasmodium falciparum, leishmania infantinum, giardia lamblia, and entamoeba histolytica. they are known to affect enzymatic activities that are required for the cellular respiration processes ...201728366172
characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa.in this study, the use of skimmed milk flocculation (smf) to simultaneously concentrate viruses, bacteria and protozoa was evaluated. we selected strains of faecal indicator bacteria and pathogens, such as escherichia coli and helicobacter pylori. the viruses selected were adenovirus (hadv 35), rotavirus (rov sa-11), the bacteriophage ms2 and bovine viral diarrhoea virus (bvdv). the protozoa tested were acanthamoeba, giardia and cryptosporidium. the mean recoveries with q(rt)pcr were 66% (hadv 3 ...201728093213
artemisinin and its derivatives in treating protozoan infections beyond malaria.parasitic protozoan diseases continue to rank among the world's greatest global health problems, which are also common among poor populations. currently available drugs for treatment present drawbacks, urging the need for more effective, safer, and cheaper drugs. artemisinin (art) and its derivatives are some of the most important classes of antimalarial agents originally derived from artemisia annua l. however, besides the outstanding antimalarial and antischistosomal activities, art and its de ...201727867026
presence of zoonotic cryptosporidium scrofarum, giardia duodenalis assemblage a and enterocytozoon bieneusi genotypes in captive eurasian wild boars (sus scrofa) in china: potential for zoonotic transmission.cryptosporidium spp., giardia duodenalis and enterocytozoon bieneusi are the main causal pathogens of gastrointestinal disease. however, there are limited reports about the prevalence of these organisms in captive eurasian wild boars worldwide. therefore, we examined the occurrence and identified the species/assemblages/genotypes of these pathogens in captive eurasian wild boars, and estimated the zoonotic potential.201728061911
occurrence of blastocystis spp. in uberaba, minas gerais, brazil.intestinal parasites are a problem for public health all over the world. the infection with blastocystis, a protozoan of controversial pathogenicity, is one of the most common among them all. in this study, the occurrence of intestinal parasites, with emphasis on blastocystis, in patients at the universidade federal do triângulo mineiro was investigated in uberaba (mg) through microscopy of direct smears and fecal concentrates using ritchie's method. feces of 1,323 patients were examined from ap ...201726200960
epidemiology of soil-transmitted helminth and intestinal protozoan infections in preschool-aged children in the amhara region of ethiopia.intestinal parasites are important contributors to global morbidity and mortality and are the second most common cause of outpatient morbidity in ethiopia. this cross-sectional survey describes the prevalence of soil-transmitted helminths and intestinal protozoa in preschool children 0-5 years of age in seven communities in the amhara region of ethiopia, and investigates associations between infection, household water and sanitation characteristics, and child growth. stool samples were collected ...201728167597
prevalence and seasonal variation of human intestinal parasites in patients attending hospital with abdominal symptoms in northern jordan.this study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern jordan. a total of 21 906 stool samples were collected over a period of 4 years (2009- 2013) from 5 government hospitals in 3 cities. samples were processed and examined microscopically and by concentration methods. parasitic infection was found in 9611 samples (44%). giardia lamblia was the most prevalent parasite (41%) followed by entamoeba histolytica (31%) and ent. coli (13 ...201728134428
the roundworm strongyloides stercoralis in children, dogs, and soil inside and outside a segregated settlement in eastern slovakia: frequent but hardly detectable parasite.a comparative study was carried out to evaluate the strongyloides stercoralis infections in children and dogs inside and outside the segregated settlement in medzev, eastern slovakia, and a survey of the soil within the settlement was included. applying the koga agar plate (kap) culture method and microscopy examination of stool samples collected from 60 roma and 21 nonroma children, no larvae of s. stercoralis were detected but eggs of three nematodes (ascaris lumbricoides, trichuris trichiura, ...201728074315
seroprevalence of five parasitic pathogens in pregnant women in ten caribbean countries.to date, published epidemiological studies of parasitic infections in humans in the caribbean region are very limited. here, we report the seroprevalence of five parasitic pathogens, including ascaris lumbricoides, entamoeba histolytica, giardia lamblia, schistosoma mansoni, and toxocara canis in 435 serum samples collected between 2008 and 2011 from pregnant women in ten caribbean islands. we tested the serum samples for igg antibodies against the five parasites by enzyme-linked immunosorbent a ...201727778108
improved diagnosis of trichuris trichiura by using a bead-beating procedure on ethanol preserved stool samples prior to dna isolation and the performance of multiplex real-time pcr for intestinal parasites.for the majority of intestinal parasites, real-time pcr-based diagnosis outperforms microscopy. however, the data for trichuris trichiura have been less convincing and most comparative studies have been performed in populations with low prevalence. this study aims to improve detection of t. trichuria dna in human stool by evaluating four sample preparation methods. faecal samples (n = 60) were collected at flores island, indonesia and examined by microscopy. aliquots were taken and a bead-beatin ...201728290266
geospatial distribution of intestinal parasitic infections in rio de janeiro (brazil) and its association with social determinants.intestinal parasitic infections remain among the most common infectious diseases worldwide. this study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of rio de janeiro, considering demographic, socio-economic, and epidemiological contextual factors.201728273080
gastrointestinal disorders next to respiratory infections as leading symptoms of x-linked agammaglobulinemia in children - 34-year experience of a single center.respiratory tract infections constitute the most frequent manifestation of x-linked agammaglobulinemia (xla). there are not many papers elucidating gastrointestinal (gi) disorders, including inflammatory bowel disease (ibd), in such patients. the aim of the study was to evaluate the occurrence of gastrointestinal disorders and ibd compared to respiratory tract infections in xla individuals.201728261296
[infection due to mycobacterium bovis in common variable immunodeficiency].common variable immunodeficiency (cvid) is an heterogeneous group of disorders characterized by impaired antibody production. it shows a wide spectrum of manifestations including severe and recurrent respiratory infections (streptococcus pneumoniae, haemophilus) and gastrointestinal (campylobacter jejuni, rotavirus and giardia lamblia). viral infections caused by herpes zoster, cytomegalovirus (cmv) and hepatitis c are rare. the opportunistic agents such as cmv, pneumocystis jirovecii, cryptococ ...201725758115
association between enteric protozoan parasites and gastrointestinal illness among hiv- and tuberculosis-infected individuals in the chowke district, southern mozambique.human immune deficiency virus (hiv) and tuberculosis (tb) infections remain major public health issues globally, particularly in sub-saharan africa. impairment of both cell-mediated and humoral immunity by hiv and/or tb infections may limit the host's defences against other pathogens, including the diarrheagenic protozoan cryptosporidium spp., giardia intestinalis, and entamoeba histolytica. during september-december 2015 a cross-sectional study was conducted to assess the prevalence and molecul ...201728302528
practical parasitology courses and infection with intestinal parasites in students.students who are working in research or educational laboratories of parasitology, as well as health care workers providing care for patients, are at the risk of becoming infected with parasites through accidental exposure. the main purpose of this study was to identify potential positive cases of intestinal parasitic infections among students who took practical parasitology courses compared with students who did not take any practical parasitology courses in lorestan university of medical scienc ...201726796769
prevalence of intestinal parasites among food handlers of sari, northern iran.parasitic infection is highly prevalent throughout the developing countries of the world. food handlers are a potential source of infection for many intestinal parasites and other enteropathogenic infections as well. the aim of this study was to determine the prevalence of intestinal parasite carriers among food handlers attending the public health center laboratory in sari, northern iran for annual check-up. the study was performed from august 2011 through february 2012. stool samples were coll ...201725923893
intestinal parasitic infections in different groups of immunocompromised patients in kashan and qom cities, central iran.intestinal parasitic infections (ipis) are important causes of morbidity and mortality in patients with immunocompromising conditions.201728362138
prevalence of strongyloides stercoralis and other intestinal parasites among institutionalized mentally disabled individuals in rasht, northern iran.we aimed to determine the status of strongyloidiasis in mentally disabled population in the institutional places in rasht city, the capital of guilan province, northern iran.201728127364
prevalence of intestinal parasitic infections in jiroft, kerman province, iran.intestinal parasitic infections have a worldwide distribution. high prevalence of intestinal parasitic infections in individuals with low socioeconomic status and environmental conditions was found. no study has ever been conducted on the prevalence of these infections in jiroft. therefore, in this study prevalence of intestinal parasitic infections was evaluated in jiroft, kerman province, iran.201728096858
synthesis and biological evaluation of newer 1,3,4-oxadiazoles incorporated with benzothiazepine and benzodiazepine moieties.a series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (staphylococcus aureus, staphylococcus pyogenes, escherichia coli, and pseudomonas aeruginosa) and fungi (candida albicans, aspergillus niger, and aspergillus clavatus) and protozoa (entamoeba histolytica, giardia lamblia, trypanosoma cruzi and leishmania mexicana). few of the selected compounds were tested for their ant ...201728182579
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