Publications

diversity and quantitative analysis of archaea in aggressive periodontitis and periodontally healthy subjects.	matarazzo f, ribeiro ac, feres m, faveri m, mayer mpa. diversity and quantitative analysis of archaea in aggressive periodontitis and periodontally healthy subjects. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01734.x. abstract: aim: to investigate the diversity, levels and proportions of archaea in the subgingival biofilm of generalized aggressive periodontitis (gagp; n=30) and periodontally healthy (ph; n=30) subjects. materials and methods: diversity was determined by sequencing arc ...	2011	21539593
diverse effects of porphyromonas gingivalis on human osteoclast formation.	porphyromonas gingivalis is associated with periodontitis, a chronic inflammatory disease of the tooth-supporting tissues. a major clinical symptom is alveolar bone loss due to excessive resorption by osteoclasts. p. gingivalis may influence osteoclast formation in diverse ways; by interacting directly with osteoclast precursors that likely originate from peripheral blood, or indirectly by activating gingival fibroblasts, cells that can support osteoclast formation. in the present study we inves ...	2011	21539907
genomic comparison of invasive and rare non-invasive strains reveals porphyromonas gingivalis genetic polymorphisms.	porphyromonas gingivalis strains are shown to invade human cells in vitro with different invasion efficiencies, varying by up to three orders of magnitude.	2011	21541093
effect of periodontopathogen lipopolysaccharides and proinflammatory cytokines on cd46, cd55, and cd59 gene/protein expression by oral epithelial cells.	membrane-anchored complement regulatory proteins (crps), including cd46, cd55, and cd59, protect host cells from complement attack. in the present study, we investigated whether periodontopathogen lipopolysaccharide and proinflammatory cytokines modulate crp gene/protein expression in human oral epithelial cells. the lipopolysaccharide of treponema denticola and tannerella forsythia were the most potent for increasing the gene expression of cd55 and cd59, and to a lesser extent cd46, after a 48- ...	2011	21545652
porphyromonas gingivalis muty is involved in the repair of oxidative stress-induced dna mispairing.	the ability for dna mismatch repair, after oxidative stress-induced dna damage, is critical for the persistence of porphyromonas gingivalis in the inflammatory environment of the periodontal pocket. our previous report demonstrated that, in contrast to other organisms, the repair of oxidative stress-induced dna damage involving 8-oxo-7,8-dihydroguanine (8-oxog) may occur by a yet-to-be described mechanism in p. gingivalis. 8-oxog does not block dna replication; rather, it mispairs with adenine, ...	2011	21545695
interaction of oral bacteria with gingival epithelial cell multilayers.	primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ...	2011	21545698
garlic allicin as a potential agent for controlling oral pathogens.	abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ...	2011	21548800
increased expression of c-reactive protein gene in inflamed gingival tissues could be derived from endothelial cells stimulated with interleukin-6.	background: epidemiological studies have suggested periodontitis as a risk factor for ischemic heart disease. high sensitive c-reactive protein (hs-crp), a predictor of cardiovascular risk, is elevated in periodontitis patients. therefore, local infection-induced elevation of systemic crp could account for the relationship between the 2 diseases. however, the underlying mechanism of crp production in the periodontal tissues has not been fully elucidated. therefore, the aim of the present study w ...	2011	21550586
identification of mono- or poly-specific monoclonal antibody to porphyromonas gingivalis heat-shock protein 60.	the aim of this study was to define the immunoreactive specificity of porphyromonas gingivalis (p. gingivalis) heat shock protein (hsp) 60 in periodontitis and atherosclerosis.	2011	21556254
relationship between herpes viruses and periodontopathogens in hiv patients with periodontitis.	aim: to verify a possible association between herpes viruses and periodontal pathogens in individuals with human immunodeficiency virus (hiv+) and periodontitis. material and methods: twenty-seven hiv+ subjects with chronic periodontitis (cp) and 23 hiv+ patients with gingivitis (g) were included in the study. probing depth, clinical attachment level, gingival index, and plaque index were recorded. blood, saliva, and subgingival plaque were processed for viral and bacterial identification. bacte ...	2011	21563945
effectiveness of ozone against periodontal pathogenic microorganisms.	huth kc, quirling m, lenzke s, paschos e, kamereck k, brand k, hickel r, ilie n. effectiveness of ozone against periodontal pathogenic microorganisms. eur j oral sci 2011; 119: 204-210. ©2011 eur j oral sci ozone has been proposed as an adjunct antiseptic in periodontitis therapy. the aim of this study was to investigate the antimicrobial effectiveness of gaseous/aqueous ozone, in comparison with that of the established antiseptic chlorhexidine digluconate (chx), against periodontal microorganis ...	2011	21564314
tlr2-dependent modulation of osteoclastogenesis by porphyromonas gingivalis through differential induction of nfatc1 and nf-{kappa}b.	osteolytic diseases including rheumatoid arthritis, osteomyelitis and periodontitis are usually associated with bacterial infections. however, the precise mechanisms by which bacteria induce bone loss still remain unclear. evidence exists that toll-like receptor (tlr) signaling regulates both inflammation and bone metabolism and that the receptor activator of nf-κb ligand (rankl) and its receptor rank are the key regulators for bone remodeling and for the activation of osteoclasts. here, we inve ...	2011	21566133
antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study.	becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ...	2011	21569094
diagnostic evaluation of a nanobody with picomolar affinity toward the protease rgpb from porphyromonas gingivalis.	porphyromonas gingivalis is one of the major periodontitis-causing pathogens. p. gingivalis secretes a group of proteases termed gingipains, and in this study we have used the rgpb gingipain as a biomarker for p. gingivalis. we constructed a naive camel nanobody library and used phage display to select one nanobody toward rgpb with picomolar affinity. the nanobody was used in an inhibition assay for detection of rgpb in buffer as well as in saliva. the nanobody was highly specific for rgpb given ...	2011	21569755
periodontopathogen and epstein-barr virus contamination affects transplanted bone volume in sinus augmentation.	background: bone microbial contamination can impair osteogenesis. human herpesviruses-associated vasculitis can cause vascular damage within the osseous graft and host. this study was conducted to substantiate specific contamination and assess the impact six months after sinus augmentation. methods: culture and polymerase chain reaction (pcr)-based identification were done on harvested bone particles and unstimulated whole saliva in a group of 30 patients undergoing maxillary sinus augmentation. ...	2011	21574831
[inhibitory effects of porphyromonas endodontalis lipopolysaccharides on proliferation and differentiation of mouse osteoblast].	to observe the effect of lipopolysaccharides (lps) extracted from porphyromonas endodontalis (pe) on osteoblast cell proliferation and the activity of alkaline phosphatase (alp) and interleukin (il)-6 secretion and to investigate the role of pe-lps in osteoblast proliferation and differentiation.	2011	21575438
oral p. gingivalis infection alters the vascular reactivity in healthy and spontaneously atherosclerotic mice.	abstract: background: considering that recent studies have demonstrated endothelial dysfunction in subjects with periodontitis and that there is no information about vascular function in coexistence of periodontitis and atherosclerosis, we assessed the impact of oral inoculation with the periodontal pathogen porphyromonas gingivalis on vascular reactivity in healthy and hypercholesterolemic apolipoprotein e-deficient (apoe) mice. in vitro preparations of mesenteric arteriolar bed were used to de ...	2011	21586133
[cloning of the glyceraldehydes 3-phosphate dehydrogenase gene of porphyromonas gingivalis and its expression in e. coli].	to clone the glyceraldehydes 3-phosphate dehydrogenase (gapdh) gene of porphyromonas gingivalis (p. gingivalis) and to induce its fusion expression in e. coli.	2011	21598499
ghrelin protects against the detrimental consequences of porphyromonas gingivalis-induced akt inactivation through s-nitrosylation on salivary mucin synthesis.	disturbances in nitric oxide synthase isozyme system and the impairment in salivary mucin synthesis are well-recognized features associated with oral mucosal inflammatory responses to periodontopathic bacterium, p. gingivalis. in this study, using rat sublingual gland acinar cells, we report that p. gingivalis lps-induced impairment in mucin synthesis and associated suppression in akt kinase activity were accompanied by a decrease in constitutive nitric oxide synthase (cnos) activity and an indu ...	2011	21603133
efficacy of taurolidine against periodontopathic species-an in vitro study.	the antimicrobial effect of taurolidine was tested against periodontopathic species in comparison to chlorhexidine digluconate in the presence or absence of serum. minimal inhibitory concentrations (mic), microbiocidal concentrations (mbc), as well as killing were determined against 32 different microbial strains including 3 porphyromonas gingivalis, 3 aggregatibacter actinomycetemcomitans, and 15 potentially superinfecting species with and without 25% v/v human serum. the mic(50) of taurolidine ...	2011	21607568
[effect of pg0839 gene of porphyromonas gingivalis on inflammatory cytokine in human oral epidermoid carcinoma kb cell.]	objective: to investigate the effect of pg0839 gene form porphyromonas gingivalis (pg) on inflammatory cytokine expression in human oral epidermoid carcinoma kb cell. methods: a mutant in the pg0839 gene of pg was created by insertional inactivation. group 1 was chanllenged with pgw83 strain, group 2 with pg0839-defective mutant, and the control group with dulbecco's modified eagle's medium only. kb cells were co-cultured with presence of bacteria for 24 h. at the time point of 0.5, 2, 6, 12 and ...	2011	21612711
identification of periodontal pathogens in healthy periimplant sites.	: the aim of this study was to evaluate the presence of periodontopathogens in subgingival periimplant sites in partially edentulous patients using polymerase chain reaction procedures, with regard to areas with clinical and radiographic signs of health and areas presenting periimplant disease.	2011	21613949
no production in raw264 cells stimulated with porphyromonas gingivalis extracellular vesicles.	this experiment was carried out in order to prove the inducible nitric oxide synthase (inos) expression and the nitric oxide (no) production in mouse macrophage cells (raw264) which were stimulated by vesicles released from porphyromonas gingivalis, and discussed about the role of vesicles in advance periodontal diseases.	2011	20646228
in vitro studies of the ablation mechanism of periodontopathic bacteria and decontamination effect on periodontally diseased root surfaces by erbium:yttrium-aluminum-garnet laser.	the erbium:yttrium-aluminum-garnet (er:yag) laser is now increasingly used in periodontal therapy. the purpose of this study was to investigate the effect of er:yag laser irradiation on the morphology of periodontopathic bacteria and to compare the bacterial elimination effect of the laser and the ultrasonic scaler on diseased root surfaces in vitro. colonies of porphyromonas gingivalis were exposed to a single-pulse er:yag laser at 40 mj and were examined by scanning electron microscopy (sem) a ...	2011	20309597
microsatellite gt polymorphism in intron 2 of human toll-like receptor (tlr) 2 gene and susceptibility to periodontitis.	as pattern recognition receptor, toll-like receptor (tlr) 2 is a signal molecule essential for the cellular response to porphyromonas gingivalis. a recently described guanine-thymine (gt) repeat microsatellite polymorphism of the human tlr2 gene leads to impaired promotor activity. a total of 380 patients with chronic periodontitis and 590 unrelated healthy control individuals of caucasian descent were genotyped for the gt repeat microsatellite polymorphism of the tlr2 gene. the mutations were i ...	2011	20232093
bacterial and viral pathogens in saliva: disease relationship and infectious risk.		2011	21134228
mechanical stress enhances production of cytokines in human periodontal ligament cells induced by porphyromonas gingivalis.	we have previously reported that human periodontal ligament (hpdl) cells produced many kinds of cytokines as a result of bacterial stimulation, including stimulation with porphyromonas gingivalis (p. gingivalis). however, the effects of mechanical stress on cytokine production in hpdl cells stimulated by periodontopathogenic bacteria are not clearly understood. in this study, we investigated the effects of mechanical stress on the production of inflammatory cytokines in hpdl cells induced by sti ...	2011	20970115
efficacy and safety of adjunctive local moxifloxacin delivery in the treatment of periodontitis.	moxifloxacin exerts excellent antibacterial activity against most putative periodontal pathogens and has been shown to kill bacteria in biofilm and host cells.	2011	20843236
il6 -174 genotype associated with aggregatibacter actinomycetemcomitans in indians.	genetic factors have recently been associated with presence of aggregatibacter actinomycetemcomitans subgingivally in populations living in industrialized countries. the aim of this study was to analyse associations between interleukin-6 (il6) single nucleotide polymorphisms and presence and levels of a. actinomycetemcomitans and other subgingival microbes in a rural indian population.	2011	20860759
proteases of porphyromonas gingivalis as important virulence factors in periodontal disease and potential targets for plant-derived compounds: a review article.	periodontitis is a common chronic inflammatory disorder of bacterial origin, which affects the tooth-supporting tissues. a wide range of evidences suggests that porphyromonas gingivalis plays a key role in the initiation and progression of chronic periodontitis. this gram-negative anaerobic bacterium produces several types of proteolytic enzymes, including gingipains, collagenases, and a dipeptidyl aminopeptidase iv. although these enzymes have physiological functions for p. gingivalis, they hav ...	2011	20955149
porphyromonas gingivalis induces rankl in t-cells.	porphyromonas gingivalis is an oral pathogen highly implicated in chronic periodontitis, a disease characterized by inflammatory destruction of the tooth-supporting alveolar bone and eventually, tooth loss. t-cell innate immune responses are actively involved in this pathological process. receptor activator of nf-κb ligand (rankl) is a cytokine that stimulates bone resorption, while its soluble decoy receptor osteoprotegerin (opg) blocks its action. this study aimed to investigate in jurkat t-ce ...	2011	20446027
fusobacterium nucleatum regulation of neutrophil transcription.	abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum.	2011	20663022
microbial communities in the tonsils of healthy pigs.	the tonsils of mammals such as humans and pigs are colonized with an extensive microbiota and are frequently the site for asymptomatic carriage of bacterial pathogens. the goal of this study was to determine the composition of the microbial community of the tonsils in healthy pigs. tonsils were collected from eight pigs from two different healthy herds. samples of the tonsils from each pig were used for culture dependent and culture independent identification of the microbial community. aerobic ...	2011	20663617
reduced expression of lipopolysaccharide-induced cxc chemokine in porphyromonas gingivalis-induced experimental periodontitis in matrix metalloproteinase-8 null mice.	matrix metalloproteinase-8 (mmp-8) is a central mediator in chronic periodontitis. recently developed mmp-8-deficient mice show an impaired polymorphonuclear neutrophil response and more severe alveolar bone loss in porphyromonas gingivalis-induced experimental periodontitis. the main mediators involved in neutrophil and monocyte/macrophage recruitment and in bone loss include lipopolysaccharide-induced cxc chemokine (lix/cxcl5), stromal-derived factor-1/cxc chemokine ligand 12 (sdf1/cxcl12) and ...	2011	20731768
porphorymonas gingivalis induces intracellular adhesion molecule-1 expression in endothelial cells through the nuclear factor-kappab pathway, but not through the p38 mapk pathway.	porphyromonas gingivalis is a major pathogen in the development and progression of periodontal disease. the aim of this study was to investigate whether endothelial intracellular adhesion molecule-1 (icam-1), an inflammation biomarker for periodontitis, could be modified by infection with either of two strains of p. gingivalis with different virulence capacities: avirulent atcc 33277 and virulent w83.	2011	20825555
a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants.	supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues.	2011	20831669
polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells.	to investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface.	2011	20798974
porphyromonas gingivalis (pg) a possible link between impaired oral health and acute myocardial infarction.	to investigate if oral health parameters were impaired in patients with myocardial infarction (mi) and if there was an association with serum antibody levels against the periodontal pathogens porphyromonas gingivalis (pg) and aggregatibacter actinomycetemcomitans (aa).	2011	19913930
animal models to study host-bacteria interactions involved in periodontitis.	animal models have distinct advantages because they can mimic cellular complexities that occur in humans in vivo and are often more accurate than in vitro studies that take place on plastic surfaces with limited numbers of cell types present. furthermore, cause and effect relationships can be established by applying inhibitors or activators or through the use of genetically modified animals. such gain or loss of function studies are often difficult to achieve in human clinical studies, particula ...	2012	22142960
[Calcium metabolism associated with oral diseases. Cardiovascular diseases and periodontal diseases].	Accumulated evidence has strongly suggested that the long-term effects of periodontal diseases can be linked to more serious systemic conditions such as cardiovascular diseases, diabetes, and complications of pregnancy. Especially, a prevalence of coronary heart disease was found to be significantly increased in patients with periodontitis after adjusting for risk factors such as smoking, diabetes, alcohol intake, obesity, and blood pressure. Furthermore, various studies have shown that Porphyro ...	2012	22201098
Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration.	Periodontal disease increases the risk of atherothrombotic disease, and high concentrations of low density lipoprotein (LDL) cholesterol are considered to be involved; however, the underlying mechanisms are largely unknown. Recent studies demonstrated that proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a critical role in circulating LDL cholesterol concentrations. The aim of the present study is to analyze serum PCSK9 concentrations and their relation to lipoprotein concentrations i ...	2012	22001517
in vitro antibacterial activity of galls of quercus infectoria olivier against oral pathogens.	the galls of quercus infectoria are commonly used in malay traditional medicine to treat wound infections after childbirth. in india, they are employed traditionally as dental applications such as that in treatment of toothache and gingivitis. the aim of the present study was to evaluate the antibacterial activity of galls of quercus infectoria olivier against oral bacteria which are known to cause dental caries and periodontitis. methanol and acetone extracts were screened against two gram-posi ...	2012	22203875
lymphangiogenesis is induced during development of periodontal disease.	lymphangiogenesis, the formation of new lymphatics, is associated with chronic inflammation and tissue injury, and its role is to enhance lymphatic flow, immune cell transport, and antigen clearance. it is unknown if lymphangiogenesis takes place during periodontal disease development, and we hypothesized that growth of lymphatic vessels occurs in gingiva during development of periodontitis in mice. inflammation was induced in gingiva with porphyromonas gingivalis gavage, and bone resorption was ...	2012	21979132
abrogation of neuraminidase reduces biofilm formation, capsule biosynthesis, and virulence of porphyromonas gingivalis.	the oral bacterium porphyromonas gingivalis is a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide. p. gingivalis exhibits neuraminidase activity. however, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence of p. gingivalis remain elusive. in this report, we found that p. gingivalis encodes a neuraminidase, pg0352 (sia(pg)). transcriptional analysis showe ...	2012	22025518
comprehensive transcriptome analysis of the periodontopathogenic bacterium porphyromonas gingivalis w83.	high-density tiling microarray and rna sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium porphyromonas gingivalis. the compiled p. gingivalis transcriptome profiles were based on total rna samples isolated from three different laboratory culturing conditions, and the strand-specific transcription profiles generated covered the entire genome, including both protein coding and noncoding regions. the transcription profiles revealed various operon s ...	2012	22037400
Highly Specific Protease-Based Approach for Detection of Porphyromonas gingivalis in Diagnosis of Periodontitis.	Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific substrates. Due to the presence of lysine and arginine residues in these substrates, it was hypothesize ...	2012	22075590
pathogenic microbes and community service through manipulation of innate immunity.	the periodontal pathogen porphyromonas gingivalis undermines major components of innate immunity, such as complement, toll-like receptors (tlr), and their crosstalk pathways. at least in principle, these subversive activities could promote the adaptive fitness of the entire periodontal biofilm community. in this regard, the virulence factors responsible for complement and tlr exploitation (gingipain enzymes, atypical lipopolysaccharide molecules, and fimbriae) are released as components of readi ...	2012	21948363
antimicrobial advances in treating periodontal diseases.	antibiotics are generally an efficient means of treating bacterial infections, and therefore are an obvious candidate in the treatment of periodontal diseases. systemically and locally administered antimicrobial agents of all kinds have been evaluated in multiple clinical trials. the vast majority of studies have tested antibiotics as adjuncts to non-surgical debridement. no regime has demonstrated superiority over systemically administered amoxicillin and metronidazole in the treatment of any c ...	2012	22142961
transition metal ions induce carnosinase activity in pepd-homologous protein from porphyromonas gingivalis.	aminoacylhistidine dipeptidase (ec 3.4.13.3; also xaa-his dipeptidase, carnosinase, or pepd) catalyzes the cleavage and release of an n-terminal amino acid, which is usually a neutral or hydrophobic residue, from an xaa-his dipeptide or degraded peptide fragment. pepd enzyme is found extensively in prokaryotes and eukaryotes, and belongs to the metallopeptidase family m20, a part of the metallopeptidase h (mh) clan. carnosine is a naturally occurring dipeptide (β-alanyl-l-histidine) present in m ...	2012	22001095
identification of the putative specific pathogenic genes of porphyromonas gingivalis with type ii fimbriae.	porphyromonas gingivalis, the key etiologic agent of periodontitis, can be classified into six types (i to v and ib) based on the fima genes that encode fima (a subunit of fimbriae). accumulated evidence indicates that p. gingivalis expressing type ii fimbriae (pg-ii) is the most frequent isolate from severe periodontitis cases and is more virulent than other types of p. gingivalis. however, during the pg-ii infection process, which specific virulence factors play the key role is still unclear. ...	2012	22257441
periodontitis, porphyromonas, and the pathogenesis of rheumatoid arthritis.	epidemiological data indicate a link between rheumatoid arthritis (ra) and periodontal disease (pd). in vitro and in vivo studies have sought to dissect potential mechanisms by which pd may contribute to initiation and progression of ra. however, these are both multifactorial, chronic diseases, and their complex etiologies and pathogenesis themselves remain incompletely understood. could there really be an etiological link or does this simply represent a statistical coincidence muddied by common ...	2012	22274780
microbial profile of patients with periodontitis compared with healthy subjects.	objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ...	2012	22257880
full-mouth disinfection and systemic antimicrobial therapy in generalized aggressive periodontitis: a randomized, placebo-controlled trial.	aim: the present investigation aimed to analyse clinical and microbiological effects of systemic administration of metronidazole and amoxicillin combined with the one-stage-full-mouth-disinfection protocol (osfmd) in generalized aggressive periodontitis patients (g-agp). materials and methods: thirty-nine systemically healthy patients with g-agp were consecutively included. the test group (n = 19) received amoxicillin-metronidazole combination (500 mg of each, three times a day for 7 days) and t ...	2012	22220822
impact of supragingival therapy on subgingival microbial profile in smokers versus non-smokers with severe chronic periodontitis.	the aim of this study was to assess subgingival microbiological changes in smokers versus non-smokers presenting severe chronic periodontitis after supragingival periodontal therapy (st).	2012	22232720
the effects of bovine necrotic vulvo-vaginitis on reproductive and production performance of israeli 1st calf heifers.	bovine necrotic vulvovaginitis (bnvv) is a syndrome unique to israel characterized by necrotic lesion in the caudal vagina mainly in first calf heifers after calving, associated with porphyromonas levii. the objectives of this study were to analyze the impact of bnvv on reproductive performance, milk production and survival in the heard of first calf dairy heifers in affected farms, and to verify if the effects of bnvv are severity-dependent. for assessment of the severity level a scale of 4 deg ...	2012	22225696
oral microbial biofilm stimulation of epithelial cell responses.	oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. however, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. this report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. monoinfection ...	2012	22266273
longitudinal assessment of pigtailed macaque lower genital tract microbiota by pyrosequencing reveals dissimilarity to the genital microbiota of healthy humans.	vaginal bacterial communities play an important role in human health and have been shown to influence hiv infection. pigtailed macaques (macaca nemestrina) are used as an animal model of hiv vaginal infection of women. since the bacterial microbiota could influence retrovirus infection of pigtailed macaques, the genital microbiota in 10 cycling macaques was determined by pyrosequencing. the microbiota of all macaques was poly-microbial with a median of 13 distinct genera. strikingly, the gen ...	2012	22264029
porphyromonas endodontalis in chronic periodontitis: a clinical and microbiological cross-sectional study.	although previous studies have shown the presence of porphyromonas endodontalis in chronic periodontitis associated with periapical lesions, the occurrence of this pathogen in diseased periodontal sites without periapical lesions has been poorly investigated.	2012	22232719
assessments of salivary antioxidant activity using electron spin resonance spectroscopy.	objective: in recent years, the function of saliva has been focused on evaluation of general status. the relationship between salivary antioxidant activity and periodontal disease progression is unclear. the aim of this study is to assess the relationship between periodontal disease and salivary antioxidant activity towards various reactive oxygen species (ros) using electron spin resonance (esr) technique. methods: we demonstrated that whole saliva derived rats or human subjects scavenged ros s ...	2012	22261034
periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid.	in periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (ne) and protease 3 (pr3), the extracellular activity of which is tightly controlled by endogenous inhibitors. in vitro these inhibitors are degraded by gingipains, cysteine proteases produced by porphyromonas gingivalis. the purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (gcf) in relation to periodontal i ...	2012	22230465