Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the ebv-hybridoma technique. | 1986 | 3014265 | |
| generation of human monoclonal antibodies by fusion of ebv-activated b cells to a human-mouse hybridoma. | 1986 | 3014266 | |
| in vitro immunization for the production of antigen-specific lymphocyte hybridomas. | 1986 | 3014268 | |
| [case reports of 77 htlv iii/lav-positive patients observed during one-year period. preliminary note]. | three hundred and forty-four patients at risk for htlv/lav infection were examined and seventy-seven were positive at the htlv iii/lav ab test. the clinical and laboratory study permitted to diagnose one case of aids, 26 cases of arc and 50 cases of simple positivity at the htlv iii/lav ab test. | 1986 | 3014379 |
| preferential transcription of htlv-i ltr in cell-free extracts of human t cells producing htlv-i viral proteins. | the promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin vh gene and the ltr of the human t-lymphotropic retrovirus type i were tested for their transcription activities in cell-free extracts of four cell lines; hela, cess (epstein-barr virus-transformed human b cell line), mt-1 (htlv-i-infected human t cell line without viral protein synthesis), and mt-2 (htlv-i-infected human t cell line producing viral proteins). ltr was preferentially transcribe ... | 1986 | 3014437 |
| infectious complications in pediatric patients undergoing transplantation with t lymphocyte-depleted bone marrow. | we performed a retrospective study of the infectious complications in 14 pediatric patients undergoing transplantation with t lymphocyte-depleted bone marrow. the purpose of this study was to document the infectious complications encountered in these patients and to ascertain whether or not they appeared to be more susceptible to any particular infections. it was found that all 14 patients developed infectious complications following transplantation: there were a total of 24 bacterial infections ... | 1986 | 3014454 |
| two pseudo-outbreaks of infectious mononucleosis. | two outbreaks of suspected infectious mononucleosis (im) were investigated. in the first outbreak im was diagnosed in nine children attending a day care center. they had been tested in physicians' offices for heterophile antibody using rapid differential slide tests; all tests had been reported positive. on retesting, none of the suspected cases had detectable serum heterophile antibody. the initial test results had been falsely positive as a result of poor laboratory technique. in the second ou ... | 1986 | 3014455 |
| epstein-barr virus serologic testing: diagnostic indications and interpretations. | 1986 | 3014456 | |
| virus-associated hemophagocytic syndrome: relationship to herpes group viruses. | 1986 | 3014457 | |
| not all potently neutralizing, vaccine-induced antibodies to epstein-barr virus ensure protection of susceptible experimental animals. | cottontop tamarins have been immunized with a high molecular weight, epstein-barr (eb) virus membrane antigen (ma) glycoprotein (gp340) separated by monoclonal antibody immunoaffinity chromatography (mcabgp340). specific antibody production was monitored by immunofluorescence, a highly sensitive enzyme-linked immunosorbent assay (elisa), and virus neutralization tests, and was found to reach high titre after 4/5 inoculations. the animals were challenged with a 100% lymphomagenic dose of eb virus ... | 1986 | 3011324 |
| diffuse intestinal ulceration after marrow transplantation: a clinicopathologic study of 13 patients. | the cases of 13 allogeneic marrow transplant recipients who had undergone laparotomy for manifestations of severe enteritis were reviewed to determine the causes of the severe intestinal disease and to assess the relation between clinical, histologic, and microbiologic findings. laparotomies were performed a median of 63 days (range, 11 to 273 days) after transplantation for suspected peritonitis, intestinal obstruction, or bleeding. intestinal tissue was available from small bowel resections in ... | 1986 | 3011641 |
| production of human monoclonal igg and igm antibodies with anti-d (rhesus) specificity using heterohybridomas. | heterohybridomas secreting human igm and igg anti-d antibodies of the rhesus blood group system have been established by fusion of ebv-transformed anti-d secreting cells with the mouse myeloma cells x63-ag8.653. both classes of antibody reacted with all rh-positive cells, some du cells but not with rh-negative or db cells. concentrations of both antibodies reached between 25 micrograms/ml and 50 micrograms/ml in the culture supernatants. the cell lines have been maintained in culture for 14 mont ... | 1986 | 3011653 |
| anti-f(ab')2 antibodies in thrombocytopenic patients at risk for acquired immunodeficiency syndrome. | 22 homosexual or narcotic addict patients at risk for acquired immunodeficiency syndrome (aids) or with aids, were studied for the presence of antiimmunoglobulin antibodies and circulating immune complexes (20 were thrombocytopenic, 6 had aids). circulating immune complex levels were 10-fold higher than levels in normal subjects. igg anti-f(ab')2 antibodies were noted in homosexual as well as narcotic addict patients. of 16 homosexual patients, 7 had igg anti-f(ab')2 antibody of moderate to mark ... | 1986 | 3011860 |
| production of nephritic factor of the alternative complement pathway by epstein barr virus-transformed b cell lines derived from a patient with membranoproliferative glomerulonephritis. | nephritic factor of the alternative complement pathway (c3nef) is an igg autoantibody which binds to and stabilizes the c3 convertase (c3bbb) enzyme, and which has been detected mainly in sera from patients with membranoproliferative glomerulonephritis (mpgn) and partial lipodystrophy. to study the production of c3nef, mononuclear cells isolated from the peripheral blood of patients with mpgn and c3nef activity in their sera were infected with epstein barr virus (ebv) to establish active b lymph ... | 1986 | 3011894 |
| properties of a specific interleukin 1 (il 1) receptor on human epstein barr virus-transformed b lymphocytes: identity of the receptor for il 1-alpha and il 1-beta. | the properties of specific human interleukin 1 (il 1) receptors on human epstein barr virus-transformed b lymphocytes (ebv-b) were studied. purified human il 1-beta from a myelomonocytic cell line (thp-1) was labeled with 125i by the bolton-hunter method without detectable loss of biological activity. among four ebv-b cell lines tested, a pre-b cell type (vds-o) specifically bound the highest amount of 125i-il 1-beta. maximal binding was reached within 20 min at 4 degrees c. scatchard plot analy ... | 1986 | 3011895 |
| single cell cloning of epstein-barr virus transformed cells in 20-microliter hanging drops. | a simple method for cloning ebv transformants from single cells in 20-microliter hanging drops is described. the hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. the average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments. | 1986 | 3011914 |
| complement receptors (cr) and cytotoxic responses: monoclonal antibodies directed against cr1 and cr3 inhibit the generation of human allospecific and virus specific cytotoxic cells in vitro. | a variety of cellular immune responses involve complement factors which bind to specific receptors, and modulate or effect a specific reaction. monoclonal antibodies (mab) have been generated against complement receptors (cr) 1 and 3, which were utilized to investigate human allogeneic and epstein-barr virus specific cytotoxic cells in vitro. mab okm1, which binds to the c3bi cr (cr3), and mab m710, which binds to the c3b/4b cr (cr1), inhibited the generation of both allogeneic and virus specifi ... | 1986 | 3011915 |
| epstein-barr virus serology in bone marrow transplantations: a one-year retrospective study with detection of ebv igm-vca-specific antibodies. | the specific antibody response to epstein-barr virus (ebv) antigens of 41 bone marrow transplant recipients with leukemia or aplastic anemia was examined retrospectively by immunofluorescence test (if) over 1 year. we observed high titers (greater than 640) of igg-viral capsid antigen (vca) with emergence of igg-early antigen (ea) and frequent absence or low levels of epstein-barr nuclear antigen (ebna) antibodies. after absorption to remove rheumatoid factor (rf), five of the 41 recipients had ... | 1986 | 3011981 |
| establishment and characterization of a chronic infectious mononucleosislike syndrome in common marmosets. | epstein-barr virus (ebv) was inoculated into two species of marmosets. successful infection was established in the majority of the animals of one species, callithrix jacchus, as evidenced by the development of high, persistent levels of antibody against virus-specific capsid and early nonstructural proteins. antibodies also were produced against the major membrane antigen and, in some animals, against ebv nuclear antigen (ebna) 2 but not against ebna 1. this is the antibody profile normally note ... | 1986 | 3011982 |
| heterogeneity of epstein-barr virus derived from a nasopharyngeal carcinoma that has transforming and lytic properties. | the biological activities of epstein-barr virus (ebv) from a human epithelial hybrid cell line derived from a nasopharyngeal carcinoma (npc-kt) were studied. low concentrations of virus from the npc-kt cell line stimulated dna synthesis of human cord blood lymphocytes (cbl) and induced cbl to form ebv nuclear antigen-positive continuous cell lines. the cbl exposed to higher doses of virus showed stimulated dna synthesis 2 days after infection, followed by cell lysis. virus from the npc-kt cell l ... | 1986 | 3012175 |
| attachment of antinuclear antibodies to nasopharyngeal carcinoma or other cells during preparation of biopsy imprints. | the presence of epstein-barr virus (ebv) genomes in nasopharyngeal and other carcinomas or burkitt's and other b-cell lymphomas can be established by the demonstration of viral nucleic acid sequences in dna extracts from biopsy specimens, the detection of ebv-associated nuclear antigen (ebna) in biopsy imprints, and the inhibition of leukocyte migration by tumor extracts. of these techniques, the detection of ebna-positive tumor cells can be performed most readily in the laboratory. this report ... | 1986 | 3012176 |
| chronic fatigue possibly related to epstein-barr virus--nevada. | 1986 | 3012308 | |
| specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. | ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ... | 1986 | 3012359 |
| epstein-barr virus. infection and tumour induction. | 1986 | 3012366 | |
| dna sequence of the herpes simplex virus type 1 gene encoding glycoprotein gh, and identification of homologues in the genomes of varicella-zoster virus and epstein-barr virus. | we have determined the sequence of herpes simplex virus type 1 dna around the previously mapped location of sequences encoding an epitope of glycoprotein gh, and have deduced the structure of the gh gene and the amino acid sequence of gh. the unprocessed polypeptide is predicted to contain 838 amino acids, and to possess an n-terminal signal sequence and a c-terminal transmembrane sequence. temperature-sensitive mutant tsq26 maps within the predicted gh coding sequence. homologous genes were ide ... | 1986 | 3012465 |
| the a+t-rich genome of herpesvirus saimiri contains a highly conserved gene for thymidylate synthase. | herpesvirus saimiri (hvs) is the prototype member of a distinctive subset of lymphotropic herpesviruses (the gamma 2 subgroup) with a+t-rich coding sequences. in this paper, we show that cells productively infected with hvs contain high concentrations of a virus-specified thymidylate synthase (5,10-methylenetetrahydrofolate:dump c-methyltransferase, ec 2.1.1.45); we identify the active polypeptide and present the sequence of the virus gene. the predicted amino acid sequence of the 294-residue su ... | 1986 | 3012520 |
| homology between dna polymerases of poxviruses, herpesviruses, and adenoviruses: nucleotide sequence of the vaccinia virus dna polymerase gene. | a 5400-base-pair segment of the vaccinia virus genome was sequenced and an open reading frame of 938 codons was found precisely where the dna polymerase had been mapped by transfer of a phosphonoacetate-resistance marker. a single nucleotide substitution changing glycine at position 347 to aspartic acid accounts for the drug resistance of the mutant vaccinia virus. the 5' end of the dna polymerase mrna was located 80 base pairs before the methionine codon initiating the open reading frame. corre ... | 1986 | 3012524 |
| variable expression of the translocated c-abl oncogene in philadelphia-chromosome-positive b-lymphoid cell lines from chronic myelogenous leukemia patients. | the consistent cytogenetic translocation of chronic myelogenous leukemia (the philadelphia chromosome, ph1) has been observed in cells of multiple hematopoietic lineages. this translocation creates a chimeric gene composed of breakpoint-cluster-region (bcr) sequences from chromosome 22 fused to a portion of the abl oncogene on chromosome 9. the resulting gene product (p210c-abl) resembles the transforming protein of the abelson murine leukemia virus in its structure and tyrosine kinase activity. ... | 1986 | 3012546 |
| epstein barr virus-specific immune defects in patients with persistent symptoms following infectious mononucleosis. | in a number of patients recovery from infectious mononucleosis (im) following primary epstein barr virus (ebv) infection, is complicated by the persistence of symptoms for months or years. normally recovery from infectious mononucleosis is associated with the development of ebv-specific antibodies and memory cytotoxic t-cells, which are present in the peripheral blood of all normal seropositive individuals. we studied four patients who had persistent symptoms for more than two years after infect ... | 1986 | 3012622 |
| [activation of latent infections caused by the epstein-barr virus in systemic lupus erythematosus]. | 1986 | 3012745 | |
| [clinical aspects of cytomegalovirus infection in nonimmunosuppressed adults]. | the histological changes in cytomegalovirus (cmv) infection were first described by ribbert in 1881, and for years the virus was dreaded as the agent of infection in newborns. an infectious mononucleosis-like disease with negative heterophil antibodies in otherwise healthy adults was described in 1965. we present six previously healthy adults with cmv mononucleosis observed in 1984. the diagnosis was established by cmv-igm-elisa. all patients were febrile for an average of 20 days. the general s ... | 1986 | 3012771 |
| calcium modulation activates epstein-barr virus genome in latently infected cells. | in many viral infections the host cell carries the viral genome without producing viral particles, a phenomenon known as viral latency. the cellular mechanisms by which viral latency is maintained or viral replication is induced are not known. the modulation of intracellular calcium concentrations by calcium ionophores induced epstein-barr viral antigens in lymphoblastoid cell lines that carry the virus. when calcium ionophores were used in conjunction with direct activators of protein kinase c ... | 1986 | 3012779 |
| burkitt's cells can be triggered by teleocidin to secrete interferon-gamma. | the secretion of interferon (ifn)-gamma by t lymphocytes is mediated by the synthesis of interleukin 2 (il-2) and the availability of il-2 receptors. since some burkitt's lymphoma lines express tac antigen and can be triggered to secrete il-2 following activation with the new tumor promoter teleocidin, we addressed the question of whether the induction of il-2 by b lymphocytes is accompanied by the induction of ifn-gamma. ifn-gamma has not been detected in any of the 25 cell lines studied, and f ... | 1986 | 3010706 |
| changes in protoporphyrin distribution dynamics during liver failure and recovery in a patient with protoporphyria and epstein-barr viral hepatitis. | acute liver failure with cholestasis, histologic and serologic evidence of epstein-barr viral infection, and associated autoimmune hemolytic anemia occurred in a patient with lifelong protoporphyria. changes in previously established baseline protoporphyrin distribution dynamics in erythrocyte, plasma, and fecal excretion compartments were observed during the period of severe hepatic dysfunction and recovery. these changes were consistent with predictions of a previously described conceptual mod ... | 1986 | 3010717 |
| epstein-barr virus infection and hodgkin's disease. | 1986 | 3010784 | |
| [viral serology in ear diseases of presumably neuritic origin]. | a viral etiology is often advanced to explain the origin of bell's palsy, sudden deafness and certain vertigos. virology findings were compared in 72 patients with either chicken pox, herpes, zona or other "viral" infections, where the viral origin was not absolutely clear, and a population of healthy volunteers. results confirmed the indefinite viral nature of these affections. however, recent studies have shown that definite viral disorders are not always associated with an increase in antibod ... | 1986 | 3010792 |
| immunomodulating activity in supernatants from ebv immortalized lymphocytes. | our earlier work has demonstrated that ebv immortalized b lymphocytes are involved in a factor dependent autostimulatory cycle. soluble growth stimulating activity was released into culture supernatants by these growing b cells. growth enhancing (ge) media from b lymphocyte lines, immortalized by ebv infection, contained soluble factor(s) which modulated the con a response of normal human mononuclear cells. conditioned media from these lines affected the con a response in a biphasic manner, stim ... | 1986 | 3011263 |
| the impact of cyclosporine therapy on the occurrence of infection in the renal transplant recipient. | 1986 | 3008389 | |
| a japanese burkitt's lymphoma with t(2;8) and ebna. | a 56-year-old japanese man was admitted to the hospital with a large mass in his right axilla. histological investigation revealed that it was a burkitt's lymphoma. ultrastructures of the tumor cells showed immature lymphoid features with frequent lipid droplets within the cytoplasms. virological studies before the treatment revealed that the lymphoma was closely related to eb virus infection, being positive for ebna (more than 95% of all tumor cells) and igg antibodies to vca (x 5,120), ea (x 6 ... | 1986 | 3008496 |
| human lymphocyte hybridomas and monoclonal antibodies. | 1986 | 3008522 | |
| chronic epstein-barr virus infection associated with fever and interstitial pneumonitis. clinical and serologic features and response to antiviral chemotherapy. | two patients developed fever, interstitial pneumonitis, and pancytopenia associated with extremely high titers of antibody to replicative antigens of the epstein-barr virus. in contrast to most patients seropositive for epstein-barr virus, neither patient had an antibody response to the epstein-barr nuclear antigen k polypeptide. in addition, virus isolated from one patient had a deletion of the b95-8 type in the ecori c region of the genome. an etiologic relation between epstein-barr virus repl ... | 1986 | 3008616 |
| differences in ebv-specific antibody patterns at onset of infectious mononucleosis. | the ebv-specific antibody patterns of infectious mononucleosis (im) patients were analyzed in relation to the onset of symptoms and clinical parameters during the acute phase of the disease. the antibody patterns varied considerably on admission. three groups of patients were identified: one had not yet attained peak antibody titers, the second was at the peak and the third had passed the peak pattern. patients with a "peak" current pattern had significantly higher lymphocyte counts, asat, alat, ... | 1986 | 3008309 |
| rescue of a biologically active epstein-barr virus from nonproducer cells. | we recently established an epithelial/hybrid cell line, a2l/ah, by fusion of 8-azaguanine-resistant epithelial cells, ad-ah, with a nonproducer lymphoblastoid cell line, a2l, using lymphocytes derived from the human nasopharynx transformed with the b95-8 strain of epstein-barr virus. the treatment of the parental a2l lymphoid cells with iododeoxyuridine led to the expression of epstein-barr virus early antigen, but neither virus capsid antigen or induction of epstein-barr virus dna synthesis was ... | 1986 | 3004720 |
| epstein-barr virus infection associated with rhabdomyolysis and acute renal failure. | 1986 | 3004795 | |
| a monoclonal antibody that recognizes the alpha chain of hla-dr antigens. | a monoclonal antibody, hc2.1, has been generated that specifically reacts with both the denatured and the in vitro translated alpha chain of the dr antigen. although hc2.1 antibody reacted with the alpha chain of protein immunoprecipitated by two dr-specific monoclonal antibodies, l227 and lb3.1, it did not react with the alpha chain of the dq1 antigen immunoprecipitated by the monoclonal antibody, genox 3.53. the isoelectric focusing pattern of the alpha chain precipitated by hc2.1 antibody was ... | 1986 | 3005199 |
| depression correlated with cellular immunity in systemic immunodeficient epstein-barr virus syndrome (sides). | we conducted studies on the peripheral blood of 12 depressed patients with previous diagnoses of mood and/or personality disorders. these patients, and other depressives we observed informally, were resistant to infectious mononucleosis during an epidemic of that illness. all 12 had serologic evidence of a chronic or recrudescent viremia caused by the epstein-barr virus (ebv), the infectious agent in infectious mononucleosis. additional evidence that ebv viremia may be causally related to depres ... | 1986 | 3005245 |
| a simple method for the production of specific antiserum to the second nuclear antigen (ebna-2) of epstein-barr virus (ebv). | a simple technique for raising specific antiserum to the native molecule of epstein-barr virus (ebv)-encoded nuclear antigen-2 (ebna-2) from raji cells is described. the procedure involves the use of immunoblotting to identify the ebna-2 polypeptide followed by subsequent excision from an sds-gel and immunization of experimental animals. the anti-ebna-2 antiserum recognized a single polypeptide of 86-87 kda on immunoblots prepared from extracts of ebv-positive raji or b95-8 cells, while it did n ... | 1986 | 3005420 |
| infection with two genotypes of epstein-barr virus in an infant with aids and lymphoma of the central nervous system. | 1986 | 3005427 | |
| typing of herpes simplex virus with synthetic dna probes. | three single-stranded oligonucleotide probes, 22 bases long, homologous to unique regions of herpes simplex virus (hsv) types 1 (hsv-1) and 2 (hsv-2) and a region common to both were chemically synthesized with use of a modified phosphochloridite protocol. for hybridization experiments each probe was labeled with use of polynucleotide kinase and [gamma-32p] atp to a specific activity of approximately 2 x 10(9) cpm/micrograms. two hundred one clinical isolates of hsv (96 hsv-1 and 105 hsv-2) coll ... | 1986 | 3005436 |
| frozen deglycerolized blood and transmission of epstein-barr virus. | 1986 | 3005440 | |
| growth and spread in nude mice of epstein-barr virus transformed b-cells from a chronic lymphocytic leukemia patient. | subcutaneous inoculation of epstein-barr virus (ebv) transformed peripheral blood b-lymphocytes (pbl) from an untreated chronic lymphocytic leukemia (cll) patient produced progressively growing lethal tumors in 4 of 11 whole body irradiated (440 rads) nude mice. in one tumor bearing mouse there was splenomegaly and generalized enlargement of lymph nodes. chromosomal analysis and membrane immunofluorescence revealed that cells in all the 4 s.c. tumors and a proportion of cells in the enlarged spl ... | 1986 | 3008990 |
| superinfection of epithelial hybrid cells (d98/hr-1, npc-kt, and a2l/ah) with epstein-barr virus and the relationship to the c3d receptor. | three epstein-barr virus (ebv) genome-positive epithelial/hybrid cell lines (d98/hr-1, npc-kt, and a2l/ah) were superinfected with ebv derived from p3hr-1 (hr-1), npc-kt, and b95-8 cells. the hr-1 virus is lytic and induces early antigen in superinfected raji cells; the virus is not capable of transforming b-lymphocytes. virus preparations from npc-kt cells have both transforming and early antigen-inducing properties, while b95-8 virus can only transform b-lymphocytes. it was possible to demonst ... | 1986 | 3008992 |
| a family study of the x-linked lymphoproliferative syndrome: evidence for a b cell defect contributing to the immunodeficiency. | the x-linked lymphoproliferative syndrome (xlps) is an immunodeficiency characterized by severe primary infection with the epstein barr (eb) virus, which is often fatal. this has been attributed to failure to generate t lymphocytes which are specifically cytotoxic for eb virus-infected b lymphocytes, and which develop in all normal individuals following primary infection. we have studied a kindred which carried the defective gene for xlps and have confirmed that the pattern of serum antibody res ... | 1986 | 3009060 |
| studies of ebv-lymphoid cell interactions in two patients with the x-linked lymphoproliferative syndrome: normal ebv-specific hla-restricted cytotoxicity. | two x-linked lymphoproliferative syndrome (xlp) patients with the hypogammaglobulinemia phenotype were investigated at a time remote from their primary infection with the epstein-barr virus (ebv). the lymphoblastoid cell lines derived from these patients expressed the phenotypic markers characteristic of normal mature b lymphocytes and produced normal levels of immunoglobulins (ig). these observations imply that at least some of their b cells are phenotypically normal. the natural killer (nk) ac ... | 1986 | 3009061 |
| suppression of in vitro human antithyroglobulin antibody secretion by private and cross-reactive anti-idiotypic antibodies. | this is a report on suppression of in vitro human antithyroglobulin antibody secretion in epstein-barr (eb) virus-transformed b lymphocytes by private and cross-reactive anti-idiotypic antibodies. two polyclonal anti-idiotypic antibodies, private anti-yo-id and cross-reactive anti-uc-id antibodies, were raised in rabbits. human igg, lambda antithyroglobulin antibody-producing cell line (yo3) and human igg-producing cell line (yo5), which did not contain the antithyroglobulin activity were establ ... | 1986 | 3009064 |
| the epstein-barr virus-induced production of ige by human b cells. | b cells, isolated from the blood of healthy individuals and patients allergic to pollen, produced ige when exposed to the human b-cell polyclonal activator, epstein-barr virus (ebv) in vitro and placed in culture. secreted igm and ige were measured using immunoenzymatic assays. no difference was seen between healthy donors and allergic patients in the amount of ige (or igm) secreted. cells were placed in limiting dilution cultures in order to determine the frequency of cells producing ige or igm ... | 1986 | 3009065 |
| human monoclonal antibody to purified protein derivative of tuberculin produced by hybrids constructed with epstein-barr virus-transformed b lymphocytes and mouse myeloma cells. | a method of producing human monoclonal antibody by combining somatic cell hybridization technology with the capability of epstein-barr virus (ebv) to transform human b lymphocytes is described. peripheral blood lymphocytes from a donor with positive tuberculin skin test reaction were transformed by ebv and then tested for antibody production to mycobacterial purified protein derivative (ppd) by an enzyme-linked immunosorbent assay. two ebv-transformed lymphoblastoid cell lines making igm antibod ... | 1986 | 3009205 |
| [ifn production by epstein-barr virus in human mononuclear leukocytes and suppression of the virus-induced transformation by the endogenous interferon]. | this study was undertaken to see whether or not interferon (ifn) is produced in infection with epstein-barr virus (ebv) and whether or not the ifn suppresses the transformation by ebv. when mononuclear leukocytes were infected with b95-8 ebv, ifn level reached a maximum 24 h after exposure to the virus, and a gradual decrease followed. there were apparent correlations between ebv inoculum size and ifn titer and between cell density and ifn titer. ifn was also induced by uv-inactivated ebv and he ... | 1986 | 3009290 |
| detection of igg and iga antibodies to epstein-barr virus membrane antigen in sera from patients with nasopharyngeal carcinoma and from normal individuals. | igg and iga antibodies to epstein-barr virus (ebv) membrane antigen (ma) were detected in sera from 96 npc patients and normal individuals by the indirect immunofluorescence test. for ma/igg antibody, 100% of npc patients were positive with a gmt of 1:439.7 and 97.9% of normal individuals were positive with a gmt of 1:94.7. in contrast, for ma/iga antibody, 58.3% of npc patients were positive with a gmt of 1:7.3 and none of the normal individuals were positive. there was no difference in the det ... | 1986 | 3009331 |
| genetics of the insulin receptor defect in a patient with extreme insulin resistance. | a patient with extreme insulin resistance (leprechaun/ark-1) had an 80-90% decrease in the number of insulin receptors on her circulating monocytes. in contrast, while a normal number of insulin receptors was expressed on the surface of epstein-barr (eb) virus-transformed lymphocytes from the patient, the receptors had decreased sensitivity to changes in temperature and ph. the father, who had a moderate degree of insulin resistance, resembled the patient in that his monocytes had a 60-80% decre ... | 1986 | 3009519 |
| glucocorticoid receptors in epstein-barr virus-transformed lymphocytes from patients with glucocorticoid resistance and a glucocorticoid-resistant new world primate species. | members of a previously reported family with glucocorticoid resistance and several new world primates have high plasma cortisol concentrations without any signs of glucocorticoid excess. the glucocorticoid receptor in circulating leukocytes and cultured skin fibroblasts from these patients and the animals is characterized by a decreased affinity for dexamethasone. on the other hand, the cell content of receptor is similar to that of corresponding tissues of normal humans. detailed biochemical-bi ... | 1986 | 3009520 |
| human monoclonal autoantibodies that react with both pancreatic islets and thyroid. | transformation of human peripheral blood lymphocytes with epstein-barr virus and rapid screening on rat insulinoma cells by an enzyme-linked immunosorbent assay were used to identify monoclonal autoantibodies that reacted with human pancreatic islets. six such monoclonal autoantibodies were isolated and cloned. all six also were found to react with human thyroid. it is concluded that lymphocytes able to make autoantibodies that react with both the pancreas and thyroid are common in the human b c ... | 1986 | 3009548 |
| release of lymphokines after epstein barr virus infection in vitro. i. sources of and kinetics of production of interferons and interleukins in normal humans. | infection of human lymphocytes with epstein barr virus (ebv) activates the release of lymphokines. previous experiments have emphasized the ability of interferon-gamma (ifn-gamma) to prevent ebv-induced b cell transformation. however, the factors that regulate ifn-gamma synthesis and release during in vitro ebv infection are controversial. in the present investigation we have systematically evaluated the kinetics of production, cellular origins, and accessory cell requirements for ifn-alpha and ... | 1986 | 3009608 |
| release of lymphokines after infection with epstein barr virus in vitro. ii. a monocyte-dependent inhibitor of interleukin 1 downregulates the production of interleukin 2 and interferon-gamma in rheumatoid arthritis. | epstein barr virus (ebv)-infection of normal peripheral blood mononuclear cells (pbmc) in vitro induces ifn-alpha secretion from b cell and natural killer (nk) cell populations, and ifn-gamma secretion from t cells. ifn-gamma depends on prior elaboration of il 2 and il 1 that originates from monocytes and nk cells. pbmc from rheumatoid arthritis (ra) patients released moderately elevated levels of ifn-alpha (236 +/- 62 u/ml vs 168 +/- 34 in normals). in contrast, ifn-gamma was significantly lowe ... | 1986 | 3009609 |
| incorporation of the purified epstein barr virus/c3d receptor (cr2) into liposomes and demonstration of its dual ligand binding functions. | the 145-kda molecule that has been identified as the c3d receptor cr2 was isolated from lysates of raji cells by affinity chromatography by using the monoclonal antibody (moab)hb-5. the purified protein was incorporated into 14c-phosphatidylcholine liposomes by deoxycholate dialysis followed by flotation on discontinuous sucrose gradients. incorporation of the receptor was verified by testing the gradient fractions for cr2 by an enzyme-linked immunosorbent assay. liposomes were shown to be unila ... | 1986 | 3009615 |
| biochemical and antigenic analysis of the epstein barr virus/c3d receptor (cr2). | four monoclonal antibodies (okb7, hb-5, ab-1, and anti-b2) that recognize a 145-kda b cell-specific membrane structure have markedly different abilities to 1) inhibit c3d and ebv binding to b cells, 2) immunoprecipitate a 145-kda b cell protein, and 3) stimulate b cell proliferation and differentiation into ig-secreting cells. this study was initiated to determine whether these four monoclonal antibodies (moab) react with the same protein; a related goal was to determine whether the structure(s) ... | 1986 | 3009616 |
| quantitation of epstein-barr virus (ebv)-determined nuclear antigen (ebna) by a two-site enzyme immunoassay, in parallel with ebv-dna. | a two-site enzyme (tse) immunoassay was developed for the quantitation of the epstein-barr virus (ebv)-determined nuclear antigen (ebna) using a rabbit serum raised against a synthetic peptide derived from the bamhi k region of the viral genome. comparison of 12 ebna-positive and 3 negative cell lines proved that the test was ebv-specific. a dot-blot assay utilizing cloned and nick translated ebv-dna bamhi m fragment confirmed the ebv-carrier status of the ebna-positive lines. the results obtain ... | 1986 | 3009623 |
| evaluation of antibodies to epstein-barr virus in italian patients with nasopharyngeal carcinoma. | an association of epstein-barr virus (ebv) with nasopharyngeal carcinoma (npc) has been established serologically in italian patients. the finding of iga antibodies to ebv-capsid antigen (vca) and to early antigen (ea) showed a high degree of correlation for patients with npc, thereby confirming previous reports. in addition, when considered together, iga anti-vca and igg anti-ea antibody titres appeared to distinguish npc-patients from those in control populations. poorly differentiated and und ... | 1986 | 3009628 |
| sphingomyelinases and niemann-pick disease. | in the first part the properties of normal mammalian sphingomyelinases are reviewed: the lysosomal acid sphingomyelinase is a polymeric glycoprotein (subunit mr between 28 000 and 70 000) which hydrolyses natural sphingomyelin, coloured and fluorescent semi-synthetic analogues (trinitrophenyl-aminolauryl-sphingomyelin and pyrenedecanoyl-sphingomyelin) and the synthetic analogue 2-n-hexadecanoylamido-nitrophenyl-phosphorylcholine. the suitability of these substrates and of synthetic fluorescent d ... | 1986 | 3009683 |
| some highlights of animal virus research in 1985. | 1986 | 3009687 | |
| deletion mutants that affect expression of epstein-barr virus nuclear antigen in cos-1 cells after gene transfer with simian virus 40 vectors containing portions of the bamhi k fragment. | we have identified sequences that affect the efficient expression of epstein-barr virus nuclear antigen (ebna 1) when the structural portion of its gene, found within the 2.9-kilobase-pair bamhi/hindiii fragment called ilf, is expressed from a simian virus 40 vector. a set of nested deletions at the bamhi end of the fragment was constructed by using bal 31 digestion, the addition of linkers, and ligation into psvod. the mutants were tested for their ability to express antigen in cos-1 monkey cel ... | 1986 | 3009849 |
| palindromic structure and polypeptide expression of 36 kilobase pairs of heterogeneous epstein-barr virus (p3hr-1) dna. | among the epstein-barr virions (ebv) produced by the p3hr-1 (hr-1) cell line are a defective subpopulation with rearranged viral dna designated heterogeneous dna (het dna). these defective virions are responsible for the capacity of hr-1 virus to induce early antigen in raji c cells and for trans activation of latent ebv in x50-7 cells. virions with het dna are independent replicons which pass horizontally from cell to cell rather than being partitioned vertically. we analyzed the structure and ... | 1986 | 3009861 |
| epidermodysplasia verruciformis-associated human papillomavirus 8: genomic sequence and comparative analysis. | human papillomavirus (hpv) 8 induces skin tumors which are at high risk for malignant conversion. the nucleotide sequence of hpv8 has been determined and compared to sequences of papillomaviruses with different oncogenic potential. the general organization of the hpv8 genome is similar to that of other types. highly conserved, genus-specific sequences were found in open reading frames (orfs) e1, e2, and l1. in orfs e6, e7, and l2, hpv8 is more distantly related, but it was possible to differenti ... | 1986 | 3009874 |
| epstein-barr virus-specific dna polymerase in virus-nonproducer raji cells. | virus-nonproducer raji cells, when induced to early antigen synthesis by 12-o-tetradecanoyl-phorbol-13-acetate and sodium butyrate, showed an increase in dna polymerase activity. this enzyme has the characteristics of a typical epstein-barr virus dna polymerase with regard to chromatographical pattern and biological properties: it is eluted from deae-cellulose at 0.08 m nacl, has a high salt resistance, is sensitive to phosphonoacetic acid and phosphonoformate, and shows a substrate preference f ... | 1986 | 3009879 |
| expression of gamma-subunit of enolase, neuron-specific enolase, in human non-neuroendocrine tumors and derived cell lines. | the gamma-subunit of 2-phospho-d-glycerate hydrolyase, e.c. 4.2.1.11 (enolase), neuron-specific enolase (nse), is present at high concentrations in neurons and neuroendocrine cells and has therefore recently been introduced as a marker for neuroendocrine tumors. by the indirect methods, immunocytochemistry and radioimmunoassay, nse has been detected also in some nonneuroendocrine tumors, a finding that could reflect technical artifacts or the capacity for nse expression in nonneuroendocrine tumo ... | 1986 | 3009968 |
| antagonism of glucocorticoid induction of epstein-barr virus early antigens by different steroids in daudi lymphoma cells. | four antiglucocorticoids, ru38486, ru5020, ru25055 and progesterone were found to antagonize the induction of latent epstein-barr virus (ebv) information by dexamethasone. the dose response studies show that the antagonization was more prominent with the synthetic steroids than with the natural hormone. specific binding characteristics of dexamethasone measured in whole cells indicate the presence of glucocorticoid receptors. total cellular receptor contents deduced from binding data give values ... | 1986 | 3009988 |
| [ebv diseases]. | epstein-barr virus (ebv) infection in early childhood is usually asymptomatic or associated with symptoms common to many other viral infections, such as pharyngitis, bronchitis or enteritis. typical infectious mononucleosis, as seen in young adult patients, is rare in early childhood. there is also a lower rate of heterophil antibody responses in young children. many complications of the infection are known, and many atypical manifestations can be identified as ebv-associated using modern ebv-sp ... | 1986 | 3010085 |
| [antiviral chemotherapy]. | after a discussion of the principles of antiviral chemotherapy, treatment and chemoprophylaxis of the following virus infections are reviewed in detail: the various manifestations of herpes simplex virus infections, varicella-zoster, cytomegalovirus infections, epstein-barr virus infections, laryngeal papillomas, and influenza a. special reference is made to the treatment of immunocompromized patients. acycloguanosine (acyclovir) has been found particularly useful in the treatment of herpes simp ... | 1986 | 3010086 |
| dna sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes. | we report the sequence of a 7800 base pair region of herpes simplex virus type 1 dna, representing approximately 0.16 to 0.20 map units in the genome. this contains sequences transcribed into a leftward oriented set of five 3' coterminal mrnas, together with two rightward transcribed flanking genes. one of the leftward genes encodes the virus's alkaline exonuclease, but the other gene products are uncharacterized. the amino acid sequence of one encoded protein suggested that it is a membrane emb ... | 1986 | 3010237 |
| chemically induced mutagenesis in a shuttle vector with a low-background mutant frequency. | we have developed a recombinant dna shuttle vector that permits the molecular analysis of mutations induced in human cells by chemical or physical mutagens. the vector is able to replicate as a plasmid in escherichia coli and in epstein-barr virus (ebv)-transformed human lymphoblastoid cell lines and contains the herpes simplex virus type 1 thymidine kinase gene (hsv tk) as the target for mutagenesis studies. after introduction of the vector into an ebv-transformed lymphoblastoid cell line (lcl- ... | 1986 | 3010297 |
| iga antibodies to epstein-barr virus in infectious mononucleosis. | the iga anti-ebv (epstein-barr virus) response during the course of im (infectious mononucleosis) was investigated. the iga anti-vca (viral capsid antigen) response was found not to be restricted to the early acute phase of the ebv infection as is the igm anti-vca response. some patients with normal total serum iga levels did not respond with measurable ebv specific iga. these patients and those with low titers of iga anti-vca had shorter duration of sore throat than responders with high titers ... | 1986 | 3010447 |
| an epstein-barr virus transcription unit is at least 84 kilobases long. | we have studied the structure of the epstein-barr virus mrnas expressed in b95-8, a productively-infected marmoset cell line established from in vitro-infected b-lymphocytes. we constructed a cdna library from the cytoplasmic polyadenylated rnas of b95-8 in the lambda gt10 bacteriophage. we present here the analysis of a 3.5 kbp cdna containing exons transcribed from the us, ir and ul regions of the viral genome. the corresponding transcription unit is at least 84 kbp long. two exons are transcr ... | 1986 | 3008094 |
| human retrovirus-associated lymphoproliferative disorders in homosexual men. | 1986 | 3008165 | |
| the role of epstein-barr virus in lymphomas of homosexual males. | 1986 | 3008166 | |
| long-term culture of human granulocytes and granulocyte progenitor cells. | 1986 | 3008194 | |
| monoclonal antibodies: convergence of technology and application. | 1986 | 3008195 | |
| virus production and surface marker expression in human lymphocytes immortalized following dual infection with human t-cell leukemia virus and epstein-barr virus. | by co-cultivating peripheral blood lymphocytes from normal male donors with cells from a line designated lma-66vp, established from a female donor and simultaneously producing both htlv-i and ebv, 2 continuous culture lines were obtained. normal male lymphocytes were considered to be immortalized by co-cultivation because they were of male karyotype and lacked the 3q+ chromosome that was observed in all lma-66vp cells. these immortalized cultures were designated co-culture 1 and co-culture 2. af ... | 1986 | 3007374 |
| epstein-barr virus related central nervous system lymphoma in a child after renal transplantation. | in this report, we describe the development of a rapidly progressive epstein-barr virus (ebv) related cerebral lymphoma in an 11 year old girl, eight months after renal transplantation. no serological evidence for a persistent ebv infection was found, but epstein-barr nuclear antigen (ebna) could be demonstrated in the tumor. the clinical course of our patient was different from ebv-related syndromes in renal transplanted patients described in previous reports. furthermore, pathological investig ... | 1986 | 3007383 |
| 1 alpha,25-dihydroxyvitamin d3-binding macromolecules in human b lymphocytes: effects on immunoglobulin production. | previous studies have indicated that upon in vitro activation with mitogenic lectins, human peripheral blood t lymphocytes express receptors for the steroid hormone 1 alpha, 25-dihydroxyvitamin d3(1,25(oh)2d3). furthermore, the hormone can inhibit interleukin 2 production by the activated cells. in this investigation, we report that human peripheral b lymphocytes activated in vitro with the b lymphotropic epstein-barr virus (ebv) also express 1,25(oh)2d3 receptor-like macromolecules. these recep ... | 1986 | 3007606 |
| phenotype, frequency, and ebv responsiveness of human marrow b and pre-b cells. | we have purified subpopulations of b lineage cells from human adult (rib) bone marrow by cell sorting and panning. limiting dilution analysis was then used for a clonal analysis of cells able to secrete igg, iga, or igm spontaneously or after infection with ebv. nonproliferating, high rate igg or iga producers occurred at frequencies of about one per 1000 marrow mononuclear cells. their frequency and ig production was unaffected by ebv, and they appeared not to express ebna after exposure to ebv ... | 1986 | 3007613 |
| pulmonary disease in children with acquired immune deficiency syndrome and aids-related complex. | two major pulmonary diseases were defined on the basis of lung biopsies in 15 children with acquired immune deficiency syndrome (aids) or aids-related complex. pneumocystis carinii pneumonia was observed in eight children, and pulmonary lymphoid hyperplasia in six. one child had nonspecific interstitial pneumonitis. children with p. carinii pneumonia had more severe hypoxemia, with higher alveolar-arterial oxygen gradients, and higher isomorphic elevations of serum lactate dehydrogenase. clinica ... | 1986 | 3007714 |
| hemophiliac immunodeficiency: influence of exposure to factor viii concentrate, lav/htlv-iii, and herpesviruses. | the relationship between hemophiliac immunodeficiency and exposures to factor viii concentrate, lav/htlv-iii retrovirus, and infection with epstein-barr virus and cytomegalovirus was examined. exposure to factor viii concentrate was significantly correlated with decreased percentages of t helper/inducer cells, decreased t helper/suppressor cell ratios, and decreased proliferative responses to plant mitogens. lav/htlv-iii seropositivity was the primary predictor of increased percentages of hla-dr ... | 1986 | 3007715 |
| primary epstein-barr virus infections in children with aids. | 1986 | 3007716 | |
| simultaneous presence of translocations t(14;18) and t(2;8) in a case of chronic lymphocytic leukemia. | we report a patient with classical chronic lymphocytic leukemia of igm kappa phenotype and a stable clinical course, in which repeated chromosome analyses of blood lymphocytes revealed the coexistence of t(14;18), a marker often associated with follicular low grade lymphocytic lymphomas, and t(2;8), a variant of the t(8;14) typically seen in burkitt's lymphoma. both these translocations involve immunoglobulin gene regions, the t(2;8) being almost always found in patients with kappa light chain r ... | 1986 | 3006910 |
| search for possible antitumor promoters by inhibition of 12-o-tetradecanoylphorbol-13-acetate-induced epstein-barr virus activation; ursolic acid and oleanolic acid from an anti-inflammatory chinese medicinal plant, glechoma hederaceae l. | from an anti-inflammatory chinese medicinal plant, glechoma hederaceae l., two triterpene carboxylic acids, ursolic acid (ua) and oleanolic acid (oa) have been isolated as inhibitors of 12-o-tetradecanoylphorbol-13-acetate (tpa) induced epstein-barr virus (ebv) activation in raji cells. both acids significantly inhibited the activation at a 1000-fold molar ratio to tpa, and also teleocidin b-4. the dose responses of the acids were very similar to those of the antitumor promoters, retinoic acid ( ... | 1986 | 3006912 |
| [antibody response to eb viruses in patients with infectious mononucleosis. relation of the antibody response to the course of the disease]. | 1986 | 3006916 | |
| inhibition of plasmodium falciparum growth by igg antibody produced by human lymphocytes transformed with epstein-barr virus. | supernatants from epstein-barr virus (ebv)--stimulated b lymphocytes obtained from two adult gambians who were partially immune to malaria markedly inhibited the growth of plasmodium falciparum in vitro (55-95% inhibition). when 22 separate colonies were derived by micromanipulation from one of these primary cultures and their supernatants assayed, the degree of inhibition correlated with levels of igg fluorescent antibody and total igg. the inhibitory anti-p. falciparum igg immunoprecipitated a ... | 1986 | 3006952 |
| phorbol ester enhances both interleukin 2 receptor expression and immunoglobulin secretion in human epstein-barr virus-immortalized b cells. | the enhanced expression of interleukin 2 (il2) receptors by 12-o-tetradecanoylphorbol 13-acetate (tpa) on sublines of an epstein-barr virus-immortalized human b17 b cell line (m. steinitz et al., immunobiology 1979. 156: 41.) was studied by immunofluorescence using the anti-tac monoclonal antibody and by binding studies with purified radiolabeled il2. these studies show that tpa at a final concentration of 5 ng/ml greatly increased tac antigen expression on a number of sublines of b17. il2-bindi ... | 1986 | 3007165 |
| a protective human monoclonal iga antibody produced in vitro: anti-pneumococcal antibody engendered by epstein-barr virus-immortalized cell line. | human lymphocytes that produce anti-pneumococcal antibodies were separated and immortalized by epstein-barr virus and then cloned. one clone (nad-sel) produces an iga, kappa antibody which is specific for the polysaccharides of type 8 pneumococcus, while not reactive with any of the polysaccharides derived from 24 other pneumococcal strains. the antibody, which is present in the cell supernatant as monomer and polymer, binds to protein a and does not fix complement. when incubated in vitro with ... | 1986 | 3007166 |
| autocrine models of b-lymphocyte growth. ii. interleukin-1 supports the proliferation of transformed lymphoblasts but not the stimulation of resting b cells triggered through their receptors for antigen. | purified, monocyte-derived interleukin-1 (il-1) was found to provide growth support for epstein-barr virus (ebv) transformed b-lymphocytes seeded at densities below which their own autostimulatory factors were limiting. by contrast, highly purified resting b cells triggered via their receptors for antigen failed to respond to identical preparations of il-1 by dna synthesis. that successful priming of the b cells had occurred was evidenced by a transient rise in rna synthesis and the ability of t ... | 1986 | 3007333 |