Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| central venous catheter infection with bacillus pumilus in an immunocompetent child: a case report. | bacillus organisms are common laboratory contaminants. the majority of bacillus bacteraemias are transient and not clinically significant. clinically significant infection due to bacillus species is rare and mostly due to bacillus cereus infections in immuno-compromised hosts. | 2007 | 17967173 |
| efficacy of enterocin as-48 against bacilli in ready-to-eat vegetable soups and purees. | the broad-spectrum bacteriocin enterocin as-48 was tested for biopreservation of ready-to-eat vegetable foods (soups and purees) against aerobic mesophilic endospore-forming bacteria. by adding as-48 (10 microg/ml), bacillus cereus lwl1 was completely inhibited in all six vegetable products tested (natural vegetable cream, asparagus cream, traditional soup, homemade-style traditional soup, vegetable soup, and vichyssoise) for up to 30 days at 6, 15, and 22 degrees c. a collection of strains isol ... | 2007 | 17969616 |
| occurrence of bacillus cereus spores with a damaged exosporium: consequences on the spore adhesion on surfaces of food processing lines. | this study was designed to evaluate the occurrence of bacillus cereus spores with a damaged exosporium and the consequences of such damages on spore adhesion. the analysis of nine strains sporulated under optimal conditions (spo8-agar, 30 degrees c) revealed that damaged exosporia were systematically found in one strain (b. cereus d17) and occasionally in two others (b. cereus atcc 14579t and b. cereus d6). the prevalence of spores with damaged exosporia increased when sporulation occurred under ... | 2007 | 17969617 |
| [bacillus cereus pore-forming toxins hemolysin ii and cytotoxin k: polymorphism and distribution of genes among representatives of the cereus group]. | abstract-phylogenetic interrelation between 40 strains of the bacillus cereus group has been established using bcrep fingerprinting. the pcr method has shown that the frequency of occurrence of the genes of cytotoxin k (cytk) and hemolysin ii (hlyii) is 61% and 56%, respectively, and the gene of the hemolysin ii regulator (hlyiir) occurs together with hlyii. comparison of the results of fingerprinting, pcr, and rflp of the toxin genes showed that bacteria with the hlyii+ and cytk+ genotypes did ... | 2007 | 17974202 |
| exploring the evolution of the bacillus cereus group repeat element bcr1 by comparative genome analysis of closely related strains. | bcr1 is a chromosomal approximately 155 bp repeated element found uniquely and ubiquitously in the bacillus cereus group of gram-positive bacteria; it exhibits several features characteristic of mobile elements, including a variable distribution pattern between strains. here, highly similar bcr1 elements in non-conserved genomic loci are identified in a set of closely related b. cereus and bacillus thuringiensis strains near the bacillus anthracis phylogenetic cluster. it is also shown that bcr1 ... | 2007 | 17975097 |
| carbohydrazones of substituted salicylaldehydes as potential lead compounds for the development of narrow-spectrum antimicrobials. | certain substituted salicylaldehydes are known to have highly potent antimicrobial activity against bacteria and fungi, but the mechanism underlying this remarkable activity is not known, and almost nothing has been reported on the effects of further modification of the structures, such as the formation of hydrazone-type derivatives. we report now a study on the antimicrobial properties of the carbohydrazone derivatives of several substituted salicylaldehydes. the compounds studied were synthesi ... | 2007 | 17913061 |
| antimicrobial properties of substituted salicylaldehydes and related compounds. | a systematic survey of the antimicrobial properties of substituted salicylaldehydes and some related aromatic aldehydes is reported. a total of 23 different compounds, each at four different concentrations, were studied using a panel of seven microbes (aspergillus niger, bacillus cereus, candida albicans, escherichia coli, pseudomonas aeruginosa, saccharomyces cerevisiae and staphylococcus epidermidis) and employing the paper disc agar diffusion method. several aldehydes, most notably halogenate ... | 2007 | 17913062 |
| antimicrobial activity of two antitumour agents and ribonucleotide reductase inhibitors, pyridine-2-carboxaldehyde thiosemicarbazone and the acetate form of its copper(ii) chelate. | some copper chelates have potent antitumour activity, and in some cases also the free ligands have activity in vivo. yet, little is known about their antimicrobial properties. copper(ii) chelates of the thiosemicarbazones of a-n-heterocyclic carboxaldehydes constitute one important group of such agents, also their ligands having marked antitumour activity. both the ligands and chelates inhibit ribonucleotide reductase. some ligands have been or are under clinical trials as antineoplastic agents. ... | 2007 | 17913063 |
| antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent. | the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ... | 2007 | 17913083 |
| the zn2 position in metallo-beta-lactamases is critical for activity: a study on chimeric metal sites on a conserved protein scaffold. | metallo-beta-lactamases (mbetals) are bacterial zn(ii)-dependent hydrolases that confer broad-spectrum resistance to beta-lactam antibiotics. these enzymes can be subdivided into three subclasses (b1, b2 and b3) that differ in their metal binding sites and their characteristic tertiary structure. to date there are no clinically useful pan-mbetal inhibitors available, mainly due to the unawareness of key catalytic features common to all mbetal brands. here we have designed, expressed and characte ... | 2007 | 17915249 |
| surface reaction of bacillus cereus biomass and its biosorption for lead and copper ions. | in this study, the surface chemical functional groups of bacillus cereus biomass were identified by fourier transform infrared (ftir) analytical technique. it had been shown that the b. cereus cells mainly contained carboxyl, hydroxyl, phosphate, amino and amide functional groups. the potentiometric titration was conducted to explain the surface acid-base properties of aqueous b. cereus biomass. the computer program fiteql 4.0 was used to perform the model calculations. the optimization results ... | 2007 | 17915701 |
| effect of natural preservatives on the growth of histamine producing bacteria. | present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ... | 2007 | 17915763 |
| biosurfactant production and surface translocation are regulated by plcr in bacillus cereus atcc 14579 under low-nutrient conditions. | bacillus cereus atcc 14579 can respond to nutrient changes by adopting different forms of surface translocation. the b. cereus atcc 14579 deltaplcr mutant, but not the wild type, formed dendritic (branched) patterns on eps [a low-nutrient medium that contains 7.0 g k(2)hpo(4), 3.0 g kh(2)po(4), 0.1 g mgso(4).7h(2)o, 0.1 g (nh(4))(2)so(4), 0.01 g cacl(2), 0.001 g feso(4), 0.1 g nacl, 1.0 g glucose, and 125 mg yeast extract per liter] containing 0.7% agar. the dendritic patterns formed by sliding ... | 2007 | 17921286 |
| influence of the probiotic bacillus cereus var. toyoi on the intestinal immunity of piglets. | in a feeding trial, sows and piglets were fed with the probiotic bacterium bacillus cereus var. toyoi as a feed additive, and the effects on immune cell populations were examined. the development of the gut immune system was determined for piglets at the ages of 14, 28, 35 and 56 days post partum. tissue samples of the jejunum and the continuous peyer's patch were used for enumeration of intraepithelial lymphocyte populations by fluorescence activated flow cytometry and fluorescence microscopy. ... | 2007 | 17870185 |
| food-poisoning and commercial air travel. | with the introduction of budget airlines and greater competitiveness amongst all airlines, air travel has now become an extremely popular form of travel, presenting its own unique set of risks from food poisoning. foodborne illness associated with air travel is quite uncommon in the modern era. however, when it occurs, it may have serious implications for passengers and when crew are affected, has the potential to threaten safety. quality, safe, in-flight catering relies on high standards of foo ... | 2007 | 17870632 |
| structure and biological activities of beta toxin from staphylococcus aureus. | beta toxin is a neutral sphingomyelinase secreted by certain strains of staphylococcus aureus. this virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. the structure of beta toxin was determined at 2.4-a resolution using crystals that were merohedrally twinned. this structure is similar to that of the sphingomyelinases of listeria ivanovii and bacillus cereus. beta toxin belongs to the dnase i folding superfamily; in addition to sphingomyel ... | 2007 | 17873030 |
| phenolic compounds and antimicrobial activity of olive (olea europaea l. cv. cobrançosa) leaves. | we report the determination of phenolic compounds in olive leaves by reversed-phase hplc/dad, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (bacillus cereus, b. subtilis and staphylococcus aureus), gram negative bacteria (pseudomonas aeruginosa, escherichia coli and klebsiella pneumoniae) and fungi (candida albicans and cryptococcus neoformans). seven phenolic compo ... | 2007 | 17873849 |
| incidence and characterization of diarrheal enterotoxins of fecal bacillus cereus isolates associated with diarrhea. | a total of 490 stool specimens were collected from patients with diarrhea and healthy controls without diarrhea to investigate the incidence of bacillus cereus and its enterotoxins. b. cereus was found more significant in stools of persons with diarrhea than without diarrhea (9.5% versus 1.8%, p < 0.05), and was also detected more frequent but not significant in individuals aged > or =1 year and in adults than in children aged <1 year (11% and 8% versus 7.8%, p > 0.05). the hemolytic enterotoxin ... | 2007 | 17878069 |
| tentacle probes: differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time pcr. | false-positive results are a common problem in real-time pcr identification of dna sequences that differ from near neighbors by a single-nucleotide polymorphism (snp) or deletion. because of a lack of sufficient probe specificity, post-pcr analysis, such as a melting curve, is often required for mutation differentiation. | 2007 | 17932130 |
| proposal of a simplified technique for staining bacterial spores without applying heat--successful modification of moeller's method. | as the bacterial spores are difficult to stain, a number of staining techniques including their modifications have been proposed to date. most of the conventional staining procedures unexceptionally contain the step of staining with steamed dye reagent in order to increase the stainability of the spores. we made an attempt to improve the conventional moeller's methods for staining bacterial spores. | 2007 | 17933713 |
| cloning, expression and characterization of a fast self-sufficient p450: cyp102a5 from bacillus cereus. | cyp102s represent a family of natural self-sufficient fusions of cytochrome p450 and cytochrome p450 reductase found in some bacteria. one member of this family, named cyp102a1 or more traditionally p450bm-3, has been widely studied as a model of human p450 cytochromes. remarkable detail of p450 structure and function has been revealed using this highly efficient enzyme. the recent rapid expansion of microbial genome sequences has revealed many relatives of cyp102a1, but to date only two from ba ... | 2007 | 17945181 |
| asp-120 locates zn2 for optimal metallo-beta-lactamase activity. | metallo-beta-lactamases are zinc-dependent hydrolases that inactivate beta-lactam antibiotics, rendering bacteria resistant to them. asp-120 is fully conserved in all metallo-beta-lactamases and is central to catalysis. several roles have been proposed for asp-120, but so far there is no agreed consensus. we generated four site-specifically substituted variants of the enzyme bcii from bacillus cereus as follows: d120n, d120e, d120q, and d120s. replacement of asp-120 by other residues with very d ... | 2007 | 17426028 |
| multiplexed identification of blood-borne bacterial pathogens by use of a novel 16s rrna gene pcr-ligase detection reaction-capillary electrophoresis assay. | we have developed a novel high-throughput pcr-ligase detection reaction-capillary electrophoresis (pcr-ldr-ce) assay for the multiplexed identification of 20 blood-borne pathogens (staphylococcus epidermidis, staphylococcus aureus, bacillus cereus, enterococcus faecalis, enterococcus faecium, listeria monocytogenes, streptococcus pneumoniae, streptococcus pyogenes, streptococcus agalactiae, escherichia coli, klebsiella pneumoniae, haemophilus influenzae, pseudomonas aeruginosa, acinetobacter bau ... | 2007 | 17428930 |
| a new phylogenetic cluster of cereulide-producing bacillus cereus strains. | phenotypic and molecular studies have established that cereulide-producing strains of bacillus cereus are a distinct and probably recently emerged clone within the bacillus population. we analyzed a set of b. cereus strains, both cereulide producers and nonproducers, by multilocus sequence typing. consistent with earlier reports, nonproducers demonstrated high heterogeneity. most cereulide-producing strains and all flagellar antigen type h1 strains were allocated to the known sequence type of ex ... | 2007 | 17314223 |
| nanowire labeled direct-charge transfer biosensor for detecting bacillus species. | a direct-charge transfer (dct) biosensor was developed for the detection of the foodborne pathogen, bacillus cereus. the biosensor was fabricated using antibodies as the sensing element and polyaniline nanowire as the molecular electrical transducer. the sensor design consisted of four membrane pads, namely, sample application, conjugate, capture and absorption pads. two sets of polyclonal antibodies, secondary antibodies conjugated with polyaniline nanowires and capture antibodies were applied ... | 2007 | 17320373 |
| structural studies examining the substrate specificity profiles of pc-plc(bc) protein variants. | the phosphatidylcholine preferring phospholipase c from bacillus cereus (pc-plc(bc)) catalyzes the hydrolysis of phospholipids in the following order of preference: phosphatidylcholine (pc)>phosphatidylethanolamine (pe)>phosphatidylserine (ps). in previous work, mutagenic, kinetic, and crystallographic experiments suggested that varying the amino acids at the 4th, 56th, and 66th positions had a significant influence upon the substrate specificity profile of pc-plc(bc). here, we report the crysta ... | 2007 | 17324372 |
| quantification of magnetic susceptibility in several strains of bacillus spores: implications for separation and detection. | three strains of bacillus: bacillus atrophaeus (formally bacillus globigii), bacillus thuringiensis, and bacillus cereus were tested for their intrinsic magnetic susceptibility. all three strains when sporulated demonstrated significant magnetic susceptibility using an instrument referred to as cell tracking velocimetry. energy dispersive spectroscopy also confirmed the presence of paramagnetic elements, fe and mn, in the spore form of the bacteria. it was demonstrated that this magnetic suscept ... | 2007 | 17335063 |
| presence and significance of bacillus cereus in dehydrated potato products. | dehydrated potato contains bacillus cereus at a prevalences of 10 to 40% and at numbers usually less than 10(3) cfu g(-1). b. cereus in dehydrated potato is likely to be present as spores that are able to survive drying of the raw vegetable and may represent a significant inoculum in the reconstituted (rehydrated) product where conditions favor germination of, and outgrowth from, spores. holding rehydrated mashed potato alone, or as part of another product (e.g., potato-topped pie), at temperatu ... | 2007 | 17340893 |
| two hlyiir dimers bind to a long perfect inverted repeat in the operator of the hemolysin ii gene from bacillus cereus. | hlyiir is a negative transcriptional regulator of hemolysin ii gene from b. cereus. it binds to a long dna perfect inverted repeat (44bp) located upstream the hlyii gene. here we show that hlyiir is dimeric in solution and in bacterial cells. no protein-protein interactions between dimers and no significant modification of target dna conformation upon complex formation were observed. two hlyiir dimers were found to bind to native operator independently with kd level in the nanomolar range. the m ... | 2007 | 17346714 |
| [open leg fracture with bacillus cereus infection]. | to investigate leg fractures infected with bacillus cereus in a traumatology-orthopedic ward of a university hospital. | 2007 | 17629655 |
| high resolution fesem and tem reveal bacterial spore attachment. | transmission electron microscopy (tem) studies in the 1960s and early 1970s using conventional thin section and freeze fracture methodologies revealed ultrastructural bacterial spore appendages. however, the limited technology at that time necessitated the time-consuming process of imaging serial sections and reconstructing each structure. consequently, the distribution and function of these appendages and their possible role in colonization or pathogenesis remained unknown. by combining high re ... | 2007 | 17637074 |
| walnut (juglans regia l.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. | different cultivars of walnut (juglans regia l.) leaves (cv. lara, franquette, mayette, marbot, mellanaise and parisienne) grown in portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. phenolics analysis was performed by reversed-phase hplc/dad and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, querceti ... | 2007 | 17637491 |
| synthesis and antimicrobial activity of some new 1,3,4-thiadiazole and 1,2,4-triazole compounds having a d,l-methionine moiety. | new 1,3,4-thiadiazole, 5a-e, and 1,2,4-triazolecompounds 6a-c, containing a d,l-methionine moiety were synthesized by intramolecular cyclization of 1,4-disubstituted thiosemicarbazides 4a-e in acid and alkaline media, respectively. the potential antimicrobial effects of the synthesized compounds were investigated using the staphylococcus aureus atcc 25923, bacillus antracis atcc 8705, bacillus cereus atcc 10987, sarcina lutea atcc 9341 and escherichia coli atcc 25922 strains. the newly synthesiz ... | 2007 | 17693957 |
| [usefulness of the thoracoscopic surgery under local anesthesia and irrigation for the patient with bacillus cereus empyema; report of a case]. | the case was 54-year-old male with some risks such as chronic heart failure, atrial fibrillation, and liver chirrhosis. he was admitted because of severe back pain and diagnosed as empyema by preoperative thoracentesis. by thoracoscopic procedures under local anesthesia, fibrinopurulent tissues were cleaned as much as possible and 3 of chest tubes were replaced. the final diagnosis was bacillus cereus pyothorax by bacterial cultures of pleural effusion. intrathoracic cavity was cleaned with phys ... | 2007 | 17703630 |
| control of microorganisms and reduction of biogenic amines in chicken breast and thigh by irradiation and organic acids. | the effect of irradiation or organic acid treatment of raw chicken breast and thigh meat to control inoculated microorganisms and the production of biogenic amines (ba) was studied. bacillus cereus, enterobacter cloacae, and alcaligenes faecalis were selected and inoculated into raw ground chicken breast and thigh meat at approximately 10(7) cfu/g. the samples were irradiated at 0, 0.5, 1, and 2 kgy or mixed with a 0.2 m solution of acetic, citric, or lactic acid (1 ml for 10 g of meat sample) f ... | 2007 | 17704395 |
| characterization of the adenine nucleoside specific phosphorylase of bacillus cereus. | adenosine phosphorylase, a purine nucleoside phosphorylase endowed with high specificity for adenine nucleosides, was purified 117-fold from vegetative forms of bacillus cereus. the purification procedure included ammonium sulphate fractionation, ph 4 treatment, ion exchange chromatography on deae-sephacel, gel filtration on sephacryl s-300 hr and affinity chromatography on n(6)-adenosyl agarose. the enzyme shows a good stability to both temperature and ph. it appears to be a homohexamer of 164+ ... | 2007 | 17707115 |
| evidence for a dinuclear active site in the metallo-beta-lactamase bcii with substoichiometric co(ii). a new model for metal uptake. | metallo-beta-lactamases are zinc-dependent enzymes that constitute one of the main resistance mechanisms to beta-lactam antibiotics. metallo-beta-lactamases have been characterized both in mono- and dimetallic forms. despite many studies, the role of each metal binding site in substrate binding and catalysis is still unclear. this is mostly due to the difficulties in assessing the metal content and site occupancy in solution. for this reason, co(ii) has been utilized as a useful probe of the act ... | 2007 | 17715135 |
| impact of the probiotic bacteria enterococcus faecium ncimb 10415 (sf68) and bacillus cereus var. toyoi ncimb 40112 on the development of serum igg and faecal iga of sows and their piglets. | to examine the influence of two different probiotic bacteria on the humoral immune system of swine, two animal studies were carried out with sows and their litters. the sows' feed was supplemented with either enterococcusfaecium ncimb 10415 (sf68) or bacillus cereus var. toyoi ncimb 40112 beginning early in pregnancy. the total iga content in the faeces as well as the total igg concentration in the blood of the sows was recorded before and after weaning. the same parameters were determined in th ... | 2007 | 17760301 |
| bacillus acidiceler sp. nov., isolated from a forensic specimen, containing bacillus anthracis px02 genes. | research at the center for biological defense identified plasmid-borne forms of bacillus anthracis pxo2 genes in a gram-positive, endospore-forming rod, isolated from a forensic specimen considered a credible threat of harbouring anthrax. conventional, commercial and molecular-based methods indicated that the isolate (cbd 119(t)) was not b. anthracis and considered not to be a member of the bacillus cereus group. based on the 16s rrna gene sequence similarities, strain cbd 119(t) was most closel ... | 2007 | 17766868 |
| a systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food. | to determine the diagnostic accuracy of tests for the rapid diagnosis of bacterial food poisoning in clinical and public health practice and to estimate the cost-effectiveness of these assays in a hypothetical population in order to inform policy on the use of these tests. | 2007 | 17803865 |
| maintenance of sterility in 1-ml polypropylene syringes. | the sterility of syringes filled with a growth-promoting broth when stored under various temperature conditions was studied. | 2007 | 17823109 |
| ethnobotanical survey and antibacterial activity of some plants used in guinean traditional medicine. | a total of 418 healers have been interviewed in guinea, a coastal country of west africa, ranging between 7 degrees 30 and 12 degrees 30 of northern latitude and 8 degrees and 15 degrees of western longitude. plant species used by the local inhabitants to treat infectious diseases were identified using ethnobotanical, ethnographic and taxonomic methods. during these investigations, 218 plants were registered, of which the following were the most frequently used: erythrina senegalensis, bridelia ... | 2007 | 17825510 |
| synthesis, spectroscopic and biological aspects of iron(ii) complexes. | five novel coordinated complexes of iron(ii) with ciprofloxacin and neutral bidentate ligands have been prepared and characterized using elemental analyses, magnetic measurements, ir spectra, uv-vis spectral, thermogravimetric analyses, 1h-nmr and 13c-nmr. the antimicrobial activity of the individual ligands, metal salt and metal complexes with respect to bacillus subtilis, escherichia coli, bacillus cereus, staphylococcus aureus, salmonella typhi, serratia marcescens, aspergillus niger, aspergi ... | 2007 | 17847716 |
| differential analysis of bacillus anthracis after px01 plasmid curing and comprehensive data on bacillus anthracis infection in macrophages and glial cells. | bacillus anthracis is a gram-positive bacterial organism responsible for anthrax. this organism has two pathogenic plasmids: px01 and px02. the genetic function of px01, which comprises about 198 kb, is not known, except for a region called the pathogenic island, which contains three genes-pag, lef, and cya-that code for three toxic proteins. a 2-d difference gel electrophoresis (2-d dige) system was used to verify the existence of proteins controlled by the px01 plasmid, and protein regulation ... | 2007 | 17880004 |
| development of flexible antimicrobial films using essential oils as active agents. | the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ... | 2007 | 17880148 |
| enhanced secondary metabolite (tanshinone) production of salvia miltiorrhiza hairy roots in a novel root-bacteria coculture process. | salvia miltiorrhiza bunge (lamiaceae) hairy root cultures were inoculated (at 0.02 and 0.2% v/v) and co-cultured with bacillus cereus bacteria. the root biomass growth was inhibited significantly by the bacteria inoculated to the root culture on the first day (day 0) but not by the bacteria inoculated on days 14 or 21 (in a 28-day overall period). on the other hand, the growth of the bacteria in the hairy root culture was also strongly inhibited by the hairy roots, partially because of the antib ... | 2007 | 17882415 |
| influence of an oil soluble inhibitor on microbiologically influenced corrosion in a diesel transporting pipeline. | microbial degradation of the oil soluble corrosion inhibitor (osci) baker nc 351 contributed to a decrease in inhibitor efficiency. corrosion inhibition efficiency was studied by the rotating cage and flow loop methods. the nature of the biodegradation of the corrosion inhibitor was also analysed using fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. the influence of bacterial activity on the degradation of the corrosion i ... | 2007 | 17882627 |
| [influence of bacillus cereus on microbiocenosis of gastrointestinal tract in rats]. | the modifying effect of bacillus cereus on intestinal microbiocenosis was investigated in eubiotic and disbiotic female rats. qualitative and quantitative characteristics of gut and mucosal microflora from different parts of rats' intestine were studied before and after intragastral application of b. cereus suspension. the single application of b. cereus suspension resulted in appearance of this bacterium in feces and in parietal mucin from all parts of the intestine. in eubiotic rats compared w ... | 2007 | 17882837 |
| microbial population present in fermented beverage 'cauim' produced by brazilian amerindians. | the tapirapé amerindians of the tapi'itãwa tribe produce several fermented foods and beverages among them the beverage called 'cauim'. this beverage is the main staple food for infants until two years old and their parents. for producing the beverage, several substrates are used, such as: cassava, rice, corn, maize and peanuts. the fermentation using mainly cassava was accomplished and samples were collected for chemical and microbiological analysis. a progressive acidification during the fermen ... | 2007 | 17888538 |
| enzymatic reduction of azo and indigoid compounds. | a customer- and environment-friendly method for the decolorization azo dyes was developed. azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. a new reduced nicotinamide adenine dinucleotide-dependent azoreductase of bacillus cereus, which showed high potential for reduction of these dyes, was purified using a combination of ammonium sulfate precipitation and chromatography and had a molecular mass of 21.5 kda. the optimum ph of the az ... | 2007 | 17891390 |
| the exosporium of b. cereus contains a binding site for gc1qr/p33: implication in spore attachment and/or entry. | b. cereus, is a member of a genus of aerobic, gram-positive, spore-forming rod-like bacilli, which includes the deadly, b. anthracis. preliminary experiments have shown that gc1qr binds to b. cereus spores that have been attached to microtiter plates. the present studies were therefore undertaken, to examine if cell surface gc1qr plays a role in b. cereus spore attachment and/or entry. monolayers of human colon carcinoma (caco-2) and lung cells were grown to confluency on 6 mm coverslips in shel ... | 2007 | 17892212 |
| assembly of pili on the surface of bacillus cereus vegetative cells. | vegetative forms of bacillus cereus are reported to form pili, thin protein filaments that protrude up to 1 mum from the bacterial surface. pili are assembled from two precursor proteins, bcpa and bcpb, in a manner requiring a pilus-associated sortase enzyme (srtd). pili are also formed on the surface of bacillus anthracis expressing bcpa-srtd-bcpb. bcpa is distributed throughout the entire pilus, whereas bcpb appears positioned at its tip. in agreement with the hypothesis for pilus assembly in ... | 2007 | 17897374 |
| molecular and biochemical detection of fengycin- and bacillomycin d-producing bacillus spp., antagonistic to fungal pathogens of canola and wheat. | bacillus species are well known for their ability to control plant diseases through various mechanisms, including the production of secondary metabolites. bacillus subtilis dfh08, an antagonist of fusarium graminearum, and other bacillus spp. that are antagonists of common fungal pathogens of canola were screened for peptide synthetase biosynthetic genes of fengycin and bacillomycin d. specific polymerase chain reaction (pcr) primers identified b. subtilis strains dfh08 and 49 for the presence o ... | 2007 | 17898845 |
| molecular characterization of antimicrobial compound produced by lactobacillus acidophilus aa11. | approximately 63 strains of lactobacillus acidophilus were isolated from egyptian home-made cheese and examined for production of antagonism. only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. staphylococcus aureus and bacillus cereus) and pathogens (i.e. e. coli, salmonella sp. and shigella sp.). lactobacillus acidophilus aa11 produced higher antimicrobial activity with a wide range of inhibition. the agent aa11 was sensitive to proteolytic enzymes and ret ... | 2007 | 17899791 |
| effectiveness of trisodium phosphate, acidified sodium chlorite, citric acid, and peroxyacids against pathogenic bacteria on poultry during refrigerated storage. | the effects of dipping treatments (15 min) in potable water or in solutions (wt/vol) of 12% trisodium phosphate (tsp), 1,200 ppm acidified sodium chlorite (asc), 2% citric acid (ca), and 220 ppm peroxyacids (pa) on inoculated pathogenic bacteria (listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, escherichia coli, and yersinia enterocolitica) and skin ph were investigated throughout storage of chicken legs (days 0, 1, 3, and 5) at 3 +/- 1 degrees c. all chemi ... | 2007 | 17900083 |
| two new members of streptothricin class antibiotics from streptomyces qinlingensis sp. nov. | four streptothricin-group antibiotics (1~4) were isolated from the fermentation broth of streptomyces qinlingensis sp. nov. along with the known antibiotics streptothricins f (1) and d (3), two new members of this class (2, 4) were identified as 12-carbamoyl derivatives of 1 and 3, respectively, mainly by analysis of the ir, hr-ms and nmr spectral data. the antibacterial activities of 1~4 against escherichia coli (mics 3.1, 25.0, 3.1 and 12.5 microg/ml), bacillus subtilis (mics 6.3, 25.0, 3.1 an ... | 2007 | 18276997 |
| [influence of chemical analogue of extracellular microbial autoregulators on antilysozyme activity of bacteria]. | modifying action of c7-alkyloxybenzol (methylresorcin) on the antilysozyme activity (ala) of opportunistic microorganisms (bacillus cereus, klebsiella pneumoniae, and escherichia coli) was studied. c7-alkyloxybenzol (c7-aob, methylrezorcin), which was used as chemical analogue of microbial autoregulators, was added to growth medium containing microorganisms, which were cultivated until entered stationary phase. isolation of clones was performed by seeding of 24-hours broth culture on solid growt ... | 2007 | 18283730 |
| cgas: a comparative genome annotation system. | recent advances in genome sequencing technology and algorithms have made it possible to determine the sequence of a whole genome quickly in a cost-effective manner. as a result, there are more than 200 completely sequenced genomes. however, annotation of a genome is still a challenging task. one of the most effective methods to annotate a newly sequenced genome is to compare it with well-annotated and closely related genomes using computational tools and databases. comparing genomes requires use ... | 2007 | 17993671 |
| development of a microbial population within a hot-drinks vending machine and the microbial load of vended hot chocolate drink. | in order to understand the development of the microbial population within a hot-drinks vending machine a new machine was placed in a staff area of a university campus vending only hot chocolate. the machine was cleaned weekly using a detergent based protocol. samples from the mixing bowl, dispense area, and drink were taken over a 19-wk period and enumerated using plate count agar. bacillus cereus was identified using biochemical methods. vended drinks were sampled at 0, 3, 6, and 9 min after ve ... | 2007 | 17995650 |
| recipes for antimicrobial wine marinades against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica. | we have evaluated bactericidal activities against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica of several antimicrobial wine recipes, each consisting of red or white wine extracts of oregano leaves with added garlic juice and oregano oil. dose-response plots were used to determine the percentage of the recipes that resulted in a 50% decrease in colony-forming units (cfu) at 60 min (ba(50)). studies designed to optimize antibacterial activities of the ... | 2007 | 17995688 |
| genetic distribution of 295 bacillus cereus group members based on adk-screening in combination with mlst (multilocus sequence typing) used for validating a primer targeting a chromosomal locus in b. anthracis. | the genetic distribution of 295 bacillus cereus group members has been investigated by using a modified multilocus sequence typing method (mlst). by comparing the nucleic acid sequence of the adk gene fragment, isolates of b. cereus group members most related to b. anthracis may be easily identified. the genetic distribution, with focus on the b. anthracis close neighbours, was used to evaluate a new primer set for specific identification of b. anthracis. this primer set, ba5510-1/2, targeted th ... | 2007 | 17997177 |
| structure of plcr: insights into virulence regulation and evolution of quorum sensing in gram-positive bacteria. | gram-positive bacteria use a wealth of extracellular signaling peptides, so-called autoinducers, to regulate gene expression according to population densities. these "quorum sensing" systems control vital processes such as virulence, sporulation, and gene transfer. using x-ray analysis, we determined the structure of plcr, the major virulence regulator of the bacillus cereus group, and obtained mechanistic insights into the effects of autoinducer binding. our structural and phylogenetic analysis ... | 2007 | 17998541 |
| [investigation of a foodborne intoxication in a high-density penitentiary center]. | on september 25 2005, more than 100 inmates (almost 5% of the 1,800 prison population) experienced sudden onset gastroenteritis. this outbreak was the largest foodborne outbreak described in a prison population in spain. our objective was to confirm the outbreak, identify risk factors, implement control measures, and provide recommendations. | 2007 | 18001657 |
| bacillus cereus in free-stall bedding. | to increase the understanding of how different factors affect the bacterial growth in deep sawdust beds for dairy cattle, the microbiological status of bacillus cereus and coliforms in deep sawdust-bedded free stalls was investigated over two 14-d periods on one farm. high counts of b. cereus and coliforms were found in the entire beds. on average, 4.1 log(10) b. cereus spores, 5.5 log(10) b. cereus, and 6.7 log(10) coliforms per gram of bedding could be found in the upper layers of the sawdust ... | 2007 | 18024738 |
| production of nisin z by lactococcus lactis isolated from dahi. | lactococcus lactis cm1, an isolate from homemade "dahi," a traditional fermented milk from india, used maltose as carbon source to produce a high level of bacteriocin. the bacterial cell mass and the bacteriocin production correlated with the initial ph of the medium and were highest when the initial ph was 11.0. the level of bacteriocin reached its peak at the late log phase with concomitant reduction of culture ph to 4.2, regardless of the initial ph of the medium. a combination of maltose and ... | 2007 | 18025595 |
| [bacillus cereus is a microbial decomposer of 2,4-dichlorophenol]. | a microorganism degrading 2,4-dichlorophenol isolated from an aeration pond of the baikal pulp and paper mill was identified as bacillus cereus bip507 based on morphological and physiological characters as well as 16s rdna sequencing. this microorganism proved able to degrade high 2,4-dichlorophenol concentrations (up to 560 microm). | 2007 | 18038618 |
| antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria. | the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ... | 2007 | 18041957 |
| inhibition of bacillus cereus in milk fermented with kefir grains. | the effects of kefir-fermented milk were tested against a toxigenic strain of bacillus cereus. the incubation of milk with b. cereus spores plus 5% kefir grains prevented spore germination and growth of vegetative forms. in contrast, when 1% kefir grains was used, no effects were observed. the presence of metabolically active kefir grains diminished titers of nonhemolytic enterotoxin a, as assessed by enzyme-linked immunosorbent assay. during fermentation, kefir microorganisms produce extracellu ... | 2007 | 18044443 |
| characterization of peptide deformylase2 from b. cereus. | peptide deformylase (pdf) is a metalloenzyme that removes the n-terminal formyl groups from newly synthesized proteins. it is essential for bacterial survival, and is therefore-considered as a potential target for antimicrobial chemotherapy. however, some bacteria including medically relevant pathogens possess two or more def-like genes. here we have examined two pdfs from bacillus cereus. the two share only 32% sequence identity and the crystal structures show overall similarity with pdf2 havin ... | 2007 | 18047803 |
| generation of a specific marker to discriminate gacillus anthracis from other bacteria of the bacillus cereus group. | bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including b. cereus, b. mycoides, and b. thuringiensis. dna homology studies showed that b. anthracis, b. cereus, b. mycoides, and b. thuringiensis are closely related, with a high sequence homology. to establish a method to specifically detect b. anthracis in situations such as environmental contamination, we initially performed rapd-pcr with a 10-mer random primer and conf ... | 2007 | 18051303 |
| simultaneous detection and identification of bacillus cereus group bacteria using multiplex pcr. | bacillus cereus group bacteria share a significant degree of genetic similarity. thus, to differentiate and identify the bacillus cereus group efficiently, a multiplex pcr method using the gyrb and groel genes as diagnostic markers is suggested for simultaneous detection. the assay yielded a 400 bp amplicon for the groel gene from all the b. cereus group bacteria, and a 253 bp amplicon from b. anthracis, 475 bp amplicon from b. cereus, 299 bp amplicon from b. thuringiensis, and 604 bp amplicon f ... | 2007 | 18051330 |
| crystallization and preliminary x-ray diffraction analysis of bcomt2 from bacillus cereus: a family of o-methyltransferase. | o-methyltransferases (omts), one of the ubiquitous enzymes in plants, bacteria, and humans, catalyze a methyl-transfer reaction using s-adenosylmethionine and a wide range of phenolics as a methyl donor and acceptor, respectively. substrates for most bacterial omts have largely remained elusive, but recent investigation using bcomt2, an omt from bacillus cereus, suggested that ortho-dihydroxyflavonoids could serve as substrates. to elucidate the functional and structural features of bcomt2, we e ... | 2007 | 18051771 |
| detection of bacillus cereus virulence factors in commercial products of bacillus thuringiensis and expression of diarrheal enterotoxins in a target insect. | we examined isolates from 4 commercial bioinsecticides based on different strains of bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph, bcet, entfm, ents, entt). the piplc and bcet sequences were present in all the isolates; sph was found in aizawai and israelensis; entfm only in israelensis; and ents in kurstaki, ... | 2007 | 18059560 |
| biological activity of phenylpropionic acid isolated from a terrestrial streptomycetes. | the strain anu 6277 was isolated from laterite soil and identified as streptomyces sp. closely related to streptomyces albidoflavus cluster by 16s rrna analysis. the cultural, morphological and physiological characters of the strain were recorded. the strain exhibited resistance to chloramphenicol, penicillin and streptomycin. it had the ability to produce enzymes such as amylase and chitinase. a bioactive compound was isolated from the strain at stationary phase of culture and identified as 3-p ... | 2007 | 18062653 |
| identification and expression of gh-8 family chitosanases from several bacillus thuringiensis subspecies. | the gram-positive spore-forming bacterium, bacillus thuringiensis, a member of the bacillus cereus group, produces chitosanases that catalyze the hydrolysis of chitosan to chitosan-oligosaccharides (cos). although fungal and bacterial chitosanases belonging to other glycoside hydrolase (gh) families have been characterized in a variety of microorganisms, knowledge on the genetics and phylogeny of the gh-8 chitosanases remains limited. nine genes encoding chitosanases were cloned from 29 differen ... | 2007 | 18031332 |
| [emergence of beta-lactam-dependent bacillus cereus associated with prolonged treatment with cefepime in a neutropenic patient]. | antibiotic dependence in clinical isolates has been reported, albeit rarely, such as vancomycin-dependent enterococcus and beta-lactam-dependent staphylococcus saprophyticus. we report herein a clinical isolate of beta-lactam-dependent bacillus cereus. a 16-yr-old female was admitted on 8 september 2005 with neutropenic fever during chemotherapy following surgical removal of peripheral neuroectodermal tumor. she had had an indwelling chemoport since august 2004 and experienced b. cereus bacterem ... | 2007 | 18094579 |
| development of rapid real-time pcr and most-probable-number real-time pcr assays to quantify enterotoxigenic strains of the species in the bacillus cereus group. | members of the bacillus cereus group may produce diarrheal enterotoxins and could be potential hazards if they enter the food chain. therefore, a method capable of detecting all the species in the b. cereus group rather than b. cereus alone is important. we selected nhe as the target and developed a real-time pcr assay to quantify enterotoxigenic strains of the b. cereus group. the real-time pcr assay was evaluated with 60 b. cereus group strains and 28 others. the assay was also used to constru ... | 2007 | 18095430 |
| isolation and characterization of a psychrotolerant toxin producer, bacillus weihenstephanensis, in liquid egg products. | a psychrotolerant bacteria of the bacillus cereus group was found responsible for the spoilage of whole liquid egg products. by sequencing a 16s rrna region and performing a pcr amplification of specific 16s rrna and cspa signatures, a bacillus weihenstephanensis was identified. characterization of this strain shows its ability to grow in defined medium as well as in whole liquid egg at refrigerated temperatures. the strain isolated possesses genes encoding for hemolysin bl, nonhemolytic enterot ... | 2007 | 18095431 |
| bacterial contamination of sago starch in papua new guinea. | sago starch is an important food in lowland papua new guinea. extraction of the starch from the palm and storage were performed by way of traditional methods that have been used for thousands of years. currently, very little is known about the microbiology of sago starch. sago samples were collected from areas of high starch utilization and analyzed for the presence of bacterial pathogens and indicator organisms. storage methods and duration were recorded at the time of collection, and ph and wa ... | 2007 | 18095446 |
| cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments. | this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ... | 2007 | 18095448 |
| biodegradation of corrosion inhibitors and their influence on petroleum product pipeline. | the present study enlightens the role of bacillus cereus ace4 on biodegradation of commercial corrosion inhibitors (cci) and the corrosion process on api 5lx steel. bacillus cereus ace4, a dominant facultative aerobic species was identified by 16s rdna sequence analysis, which was isolated from the corrosion products of refined diesel-transporting pipeline in north west india. the effect of cci on the growth of bacterium and its corrosion inhibition efficiency were investigated. corrosion inhibi ... | 2007 | 16580829 |
| involvement of cytoplasmic membrane damage in the copper (ii)-dependent cytotoxicity of a novel naturally occurring tripyrrole. | in the presence of a nonlethal concentration of cu(ii), washed escherichia coli atcc8739 cells were killed by a novel tripyrrole 1, isolated as a red pigment from the serratia sp. cell killing was accompanied by a depletion in the potassium pools of the cells due to the damage to the cytoplasmic membrane, without any detectable dna damage as revealed by the transformed plasmid dna and phage induction assay. this revealed that the bactericidal activity of compound 1 in the presence of cu(ii) resu ... | 2007 | 17113233 |
| insight in to the phylogeny of polyhydroxyalkanoate biosynthesis: horizontal gene transfer. | polyhydroxyalkanoates (phas) are gaining more and more importance the world over due to their structural diversity and close analogy to plastics. their biodegradability makes them extremely desirable substitutes for synthetic plastics. phas are produced in organisms under certain stress conditions. here, we investigated 253 sequenced (completely and unfinished) genomes for the diversity and phylogenetics of the pha biosynthesis. discrepancies in the phylogenetic trees for phaa, phab and phac gen ... | 2007 | 17113245 |
| recovery of bacillus thuringiensis in vegetative form from the phylloplane of clover (trifolium hybridum) during a growing season. | two media were developed which specifically allow the cultivation of bacillus thuringiensis while it is in the vegetative as opposed to the spore form. using these media b. thuringiensis was shown conclusively for the first time to exist in an active form on the phylloplane. the profile of its appearance in vegetative and spore form was followed over a growing season on clover (trifolium hybridum) in the field. three simultaneous and sudden rises and declines of both spore and vegetative cell de ... | 2007 | 17005192 |
| bayesian analysis of population structure based on linked molecular information. | the bayesian model-based approach to inferring hidden genetic population structures using multilocus molecular markers has become a popular tool within certain branches of biology. in particular, it has been shown that heterogeneous data arising from genetically dissimilar latent groups of individuals can be effectively modelled using an unsupervised classification formulation. however, most currently employed models ignore potential linkage within the employed molecular information, and can the ... | 2007 | 17087977 |
| partial purification and characterization of protease of bacillus proteolyticus cfr3001 isolated from fish processing waste and its antibacterial activities. | bacillus proteolyticus cfr3001 isolated from fish processing wastes (both fresh water and marine) produced an alkaline protease. the optimum conditions for cell growth and protease production were 37 degrees c, 96 h, agitation speed of 100 rpm and medium ph 9. the partially purified protease obtained from had specific activity of 22.05 at 37 degrees c was active between 40 degrees c and 50 degrees c and lost >20% of its activity around 60 degrees c. its molecular weight was approximately 29 kda ... | 2007 | 17092708 |
| crystal structure of bacillus cereus hlyiir, a transcriptional regulator of the gene for pore-forming toxin hemolysin ii. | production of bacillus cereus and bacillus anthracis toxins is controlled by a number of transcriptional regulators. here we report the crystal structure of b. cereus hlyiir, a regulator of the gene encoding the pore-forming toxin hemolysin ii. we show that hlyiir forms a tight dimer with a fold and overall architecture similar to the tetr family of repressors. a remarkable feature of the structure is a large internal cavity with a volume of 550 a(3) suggesting that the activity of hlyiir is mod ... | 2007 | 17097673 |
| bacteriocin-like inhibitor substances produced by mexican strains of bacillus thuringiensis. | bacteriocins are antimicrobial peptides synthesized and secreted by bacteria and could potentially be used as natural food preservatives. here, we report the production of bacteriocin-like inhibitor substances (bt-blis) by five mexican strains of bacillus thuringiensis. bacillus thuringiensis subsp. morrisoni (lbit 269), b. thuringiensis subsp. kurstaki (lbit 287), b. thuringiensis subsp kenyae (lbit 404), b. thuringiensis subsp. entomocidus (lbit 420) and b. thuringiensis subsp. tolworthi (lbit ... | 2007 | 17031616 |
| complete sequence analysis of novel plasmids from emetic and periodontal bacillus cereus isolates reveals a common evolutionary history among the b. cereus-group plasmids, including bacillus anthracis pxo1. | the plasmids of the members of the bacillus cereus sensu lato group of organisms are essential in defining the phenotypic traits associated with pathogenesis and ecology. for example, bacillus anthracis contains two plasmids, pxo1 and pxo2, encoding toxin production and encapsulation, respectively, that define this species pathogenic potential, whereas the presence of a bt toxin-encoding plasmid defines bacillus thuringiensis isolates. in this study the plasmids from b. cereus isolates that prod ... | 2007 | 17041058 |
| optimization of inactivation of endospores of bacillus cereus by antimicrobial lipopeptides from bacillus subtilis fmbj strains using a response surface method. | bacillus subtilis fmbj can produce a lipopeptide antimicrobial substance, the main components of which are surfactin and fengycin. in this paper, the sensitivity of bacillus cereus to antimicrobial lipopeptides from b. subtilis fmbj was observed, and the effect of the microstructure of antimicrobial lipopeptide on spores of b. cereus was investigated. at the same time, the optimization of the inactivation of antimicrobial lipopeptides to spores of b. cereus by a response surface methodology was ... | 2007 | 17043814 |
| hybridoma ped-2e9 cells cultured under modified conditions can sensitively detect listeria monocytogenes and bacillus cereus. | lymphocyte origin hybridoma ped-2e9 cell-based cytotoxicity assay can detect virulent listeria or bacillus species, and its application in a cell-based biosensor for onsite use would be very attractive. however, maintaining enough viable cells on a sensor platform for a prolonged duration is a challenging task. in this study, key factors affecting the survival and growth of ped-2e9 cells under modified conditions were investigated. when the ped-2e9 cells were grown in media containing 5% fetal b ... | 2007 | 17043821 |
| plasmid exchanges among members of the bacillus cereus group in foodstuffs. | the bacillus cereus sensu lato group is genetically very close and possesses a remarkable plasmid gene pool that encodes a variety of functions such as virulence and self-transfer capabilities. the potential for horizontal transfer among the various subspecies of this group, which includes the human opportunistic pathogens b. cereus sensu stricto and b. anthracis as well as the biopesticide b. thuringiensis, has led to growing concerns regarding food safety and public health. in this study, the ... | 2007 | 16996631 |
| effect of environmental parameters on growth kinetics of bacillus cereus (atcc 7004) after mild heat treatment. | the influence of temperature (10 to 50 degrees c), initial ph (4.0 to 6.0) and sodium chloride concentration (0.5 to 3.0%) on the growth in nutrient broth and in meat extract of bacillus cereus after mild heat treatment (90 degrees c--10 min) was determined. b. cereus spores survived after heating and they were able to germinate and grow in both media when post-treatment conditions were favourable. heated b. cereus did not grow at 10 and 50 degrees c or in a medium with ph 4.0. decreasing ph val ... | 2007 | 16978725 |
| the activity of the dinuclear cobalt-beta-lactamase from bacillus cereus in catalysing the hydrolysis of beta-lactams. | metallo-beta-lactamases are native zinc enzymes that catalyse the hydrolysis of beta-lactam antibiotics, but are also able to function with cobalt(ii) and require one or two metal-ions for catalytic activity. the hydrolysis of cefoxitin, cephaloridine and benzylpenicillin catalysed by cobcii (cobalt-substituted beta-lactamase from bacillus cereus) has been studied at different phs and metal-ion concentrations. an enzyme group of pk(a) 6.52+/-0.1 is found to be required in its deprotonated form f ... | 2007 | 16961465 |
| polyhydroxyalkanoate (pha) biosynthesis from structurally unrelated carbon sources by a newly characterized bacillus spp. | a newly acquired polyhydroxyalkanoate (pha) producing bacillus spp. was identified to be a strain of bacillus cereus using a range of microbiological and molecular techniques. this strain, named b. cereus spv, was found to be capable of using a wide range of carbon sources including glucose, fructose, sucrose, various fatty acids and gluconate for the production of phas, an advantage for the commercial production of the polymers. the media used for the polymer production was novel in the context ... | 2007 | 16956686 |
| prevalence of bacillus cereus in dried milk products used by chilean school feeding program. | the prevalence of bacillus cereus, in a total of 381 samples of dried milk products (milk with rice, milk substitute, milk powder, milk-cereal-rice, pudding milk, flan, and mousse) used by the chilean school feeding program, was investigated. the potential of 94 selected isolates of b. cereus to produce diarrhoeal enterotoxin (by the bcet-rpla test) in bhi culture, as well as the ability of enterotoxigenic-strains to grow at psychrotrophic temperatures were also verified. b. cereus was found in ... | 2007 | 16943088 |
| antifungal activity and enhancement of plant growth by bacillus cereus grown on shellfish chitin wastes. | bacillus cereus qq308 produced antifungal hydrolytic enzymes, comprising chitinase, chitosanase and protease, when grown in a medium containing shrimp and crab shell powder (scsp) produced from marine waste. the growth of the plant-pathogenic fungi fusarium oxysporum, fusarium solani, and pythium ultimum were considerably affected by the presence of the qq308 culture supernatant. the supernatant inhibited spore germination and germ tube elongation of f. oxysporum, f. solani, and p. ultimum. the ... | 2007 | 16797180 |
| production and partial characterization of dehairing protease from bacillus cereus mcm b-326. | bacillus cereus mcm b-326, isolated from buffalo hide, produced an extracellular protease. maximum protease production occurred (126.87+/-1.32 u ml(-1)) in starch soybean meal medium of ph 9.0, at 30 degrees c, under shake culture condition, with 2.8 x 10(8) cells ml(-1) as initial inoculum density, at 36 h. ammonium sulphate precipitate of the enzyme was stable over a temperature range of 25-65 degrees c and ph 6-12, with maximum activity at 55 degrees c and ph 9.0. the enzyme required ca(2+) i ... | 2007 | 16782331 |
| decontamination of gum arabic with gamma-rays or electron beams and effects of these treatments on the material. | samples of gum arabic were irradiated to 2.5, 5.0 and 10.0 kgy with gamma-rays and electrons for a comparison of the relative effectiveness of these two treatments and for investigation of the effects of these doses of radiation on the material. the initial raw samples were contaminated with various strains of bacteria, including fungi and spore-forming bacteria (such as enteroccus faecalis, bacillus cereus and closstridum perfringens). the samples were completely decontaminated by irradiation t ... | 2007 | 16904901 |
| induction of pullulanase production in bacillus cereus fdta-13. | studies were carried out on the production of pullulanase by a newly isolated strain bacillus cereus fdta-13. high titres of the enzyme were obtained in a medium containing branched polysaccharides. to further enhance the yield, induction of pullulanase using conventional inducers were studied. maltooligosaccharides (maltose to maltotetraose) when added in the medium individually, or in a 1:1 combination of maltotriose and maltotetraose resulted different levels of pullulanase compared to contro ... | 2007 | 16697182 |