Publications

[postoperative eviscerations].		2014	4264193
the occurrence of nuclear masses in the pulmonary capillary bed in infants.		2014	4790380
ergonomic aspects of crew seats in transport aircraft.		2014	4272712
ergonomic aspects of crew seats in transport aircraft.		2014	4272712
[circadian rhythms of induced ethanolemia parameters in six healthy young adult males].		2014	4210897
routine operative cholangiography.		2014	4193335
activation of membrane-bound adenylate cyclase by glucagon in neurospora crassa.	membrane-bound adenylate cyclase in neurospora crassa is activated by glucagon. half-maximal effect is observed at hormone concentrations of about 10 nm. after solubilization of the enzyme with lubrol-px, the glucagon effect is lost. incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (ec 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (ec 2.4.1.1) and in the rate of glycogenolysis.	2014	4263508
effects of some drugs applied topically to the nasal mucosa before nasal provocation tests with allergen.		2014	4154695
[health interests and preferred health education media as evaluated by adults].		2014	4141801
letter: net reproduction-rates.		2014	4128745
demonstration of synaptic interactions in central neurons on a simple electrical circuit.		2014	4127502
[the effect of heparin on the incidence of arrhythmias after acute myocardial infarction (author's transl)].		2014	4136215
primary polydipsia in thyrotoxicosis.		2014	4111512
[transplantation of bone marrow in pediatrics].		2014	4897429
[the problem of suppurative grafts in recurrent eventrations. its therapy using stainless steel mesh].		2014	4243457
[mass communication media and their social impact].		2014	4186927
[isolation from a natural population of strains of drosophila with increased radiosensitivity].		2014	4219924
[prognosis of myocardial infarction. introduction].		2014	4015010
leads from the mmwr. update: acquired immunodeficiency syndrome--united states.		2014	3999320
[movement course and change of wall thickness in dyskinetic and akinetic myocardial regions].		2014	4665452
british medical association attacks cigarette smoking.		2014	3978493
competencies for mainstreaming secondary level learning disabled students.		2014	3950520
[results of the treatment of lymphatic neoplasms with vepeside].		2014	3911173
[history of the names for syphilis].		2014	3911633
surface expression of protein a on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes.	magnetosomes are membrane-enclosed magnetite nanocrystals synthesized by magnetotactic bacteria (mtb). they display chemical purity, narrow size ranges, and species-specific crystal morphologies. specific transmembrane proteins are sorted to the magnetosome membrane (mm). mamc is the most abundant mm protein of magnetospirillum gryphiswaldense strain msr-1. mamf is the second most abundant mm protein of msr-1 and forms stable oligomers. we expressed staphylococcal protein a (spa), an immunoglobu ...	2014	24765089
pellino protein from pacific white shrimp litopenaeus vannamei positively regulates nf-κb activation.	pellino, named after its property that binds pelle (the drosophila melanogaster homolog of irak1), is a highly conserved e3 class ubiquitin ligase in both vertebrates and invertebrates. pellino interacts with phosphorylated irak1, causing polyubiquitination of irak1, and plays a critical upstream role in the toll-like receptor (tlr) pathway. in this study, we firstly cloned and identified a crustacean pellino from pacific white shrimp litopenaeus vannamei (lvpellino). lvpellino contains a putati ...	2014	24463313
molecular characterization and functional analysis of cactin gene from litopenaeus vannamei.	cactin (cactus interactor) is a conserved protein which was initially discovered as a novel interactor of drosophila ikb protein cactus. cactin was always characterized as a negative regulator of many different developmental processes, but only found to play an immune role in humans. to better know the immune function of cactin gene, litopenaeus vannamei cactin (lvcactin) was identified and characterized in this study. the full-length cdna of lvcactin is 3, 150 bp long, with an open reading fram ...	2014	25462455
iron shortage and bile salts play a major role in the expression of ompk gene in vibrio anguillarum.	the outer membrane protein k, ompk first identified in vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage kvp40 infecting members of the vibrionaceae. in the study, the effect of culture conditions on the expression of ompk in v. anguillarum was studied using real-time pcr. the expression increased significantly in the presence of bile salts and iron chelating agent 2, 2' bipyridine, suggesting a role for this protein in bile resistance and also in iron ac ...	2014	25033659
occurrence of virulence genes among vibrio cholerae and vibrio parahaemolyticus strains from treated wastewaters.	pathogenic vibrio species are an important cause of foodborne illnesses. the aim of this study was to describe the occurrence of potentially pathogenic vibrio species in the final effluents of a wastewater treatment plant and the risk that they may pose to public health. during the 1-year monitoring, a total of 43 vibrio strains were isolated: 23 vibrio alginolyticus, 1 vibrio cholerae, 4 vibrio vulnificus, and 15 vibrio parahaemolyticus. the pcr investigation of v. parahaemolyticus and v. chole ...	2014	25023745
multiplex pcr assays for the detection of vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae with an internal amplification control.	a multiplex polymerase chain reaction (pcr) assay that can simultaneously detect 4 major vibrio spp., vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae, in the presence of an internal amplification control (iac) was developed. species-specific pcr primers were designed based on the gyrb gene for v. alginolyticus, the collagenase gene for v. parahaemolyticus, the vvha gene for v. vulnificus, and the ompw gene for v. cholerae. additionally, an iac primer pair wa ...	2014	24731836
vibrio parahaemolyticus and its specific bacteriophages as an indicator in cockles (anadara granosa) for the risk of v. parahaemolyticus infection in southern thailand.	correlation between the numbers of vibrio parahaemolyticus and its specific bacteriophages in cockles was investigated from june 2009 to may 2010 in hat yai, songkhla, thailand. cockles obtained monthly from a local market were sampled to determine the numbers of v. parahaemolyticus and bacteriophages that could form plaques on ten strains of pandemic and nonpandemic v. parahaemolyticus. in addition, v. parahaemolyticus isolates from clinical samples from hat yai hospital over the same period we ...	2014	24682339
depuration times of donax trunculus and tapes decussatus.	the present study was performed to determine the depuration time and ability of donax trunculus (wedge clam) and tapes decussatus (carpet shell) contaminated with escherichia coli, salmonella enterica subsp. enterica serovar typhimurium and vibrio parahaemolyticus. clams were contaminated with each bacterium at the level of 7.0 - 8.0 log10 cfu/g. after contamination, clams were analyzed every 3 h in the first 24 h time period and every 6 h until the 72nd hour. during the depuration process of bo ...	2014	25477938
efficacy of neutral electrolyzed water for reducing pathogenic bacteria contaminating shrimp.	pathogenic contamination is a food safety concern. this study was conducted to investigate the efficacy of neutral electrolyzed water (new) in killing pathogens, namely, vibrio parahaemolyticus, vibrio vulnificus, salmonella enteritidis, and escherichia coli in shrimp. pure cultures of each pathogen were submerged separately in new containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. for each concentration, three submersion times were tested: 1, 3, and 5 min. the popul ...	2014	25474069
[purification and dna-binding of toxr truncated protein of vibrio parahaemolyticus].	the dna-binding domain of toxr protein of vibrio parahaemolyticus was expressed using the escherichia coli bl21lambdade3 protein expression system, and its dna-binding activity was characterized.	2014	25345029
detection and identification of six foodborne bacteria by two-tube multiplex real time pcr and melting curve analysis.	this study is aimed to develop a two-tube melting curve-based multiplex real time pcr assay (mcmrt-pcr) for the simultaneous detection of six common foodborne pathogenic bacteria (diarrhoeagenic escherichia coli, salmonella, and shigella in tube 1, staphylococcus aureus, vibrio parahaemolyticus, and listeria monocytogenes in tube 2).	2014	25341812
a novel, broad-range, ctxφ-derived stable integrative expression vector for functional studies.	ctxφ, a filamentous vibriophage encoding cholera toxin, uses a unique strategy for its lysogeny. the single-stranded phage genome forms intramolecular base-pairing interactions between two inversely oriented xerc and xerd binding sites (xbs) and generates a functional phage attachment site, attp(+), for integration. the attp(+) structure is recognized by the host-encoded tyrosine recombinases xerc and xerd (xercd), which enables irreversible integration of ctxφ into the chromosome dimer resoluti ...	2014	25225263
occurrence of vibrio and salmonella species in mussels (mytilus galloprovincialis) collected along the moroccan atlantic coast.	this study reports the occurrence of different vibrio and salmonella species in 52 samples of mytilus galloprovincialis collected from four sites along the atlantic coast between agadir and essaouira (anza, cap ghir, imssouane and essaouira). the level of escherichia coli (e. coli) was also determined to evaluate the degree of microbial pollution in the investigated areas. in this study three methods were used : afnor nf en iso 6579 v08-013 for salmonella spp., the provisional method routinely u ...	2014	24936389
alternative sigma factor rpoe is important for vibrio parahaemolyticus cell envelope stress response and intestinal colonization.	vibrio parahaemolyticus is a halophile that inhabits brackish waters and a wide range of hosts, including crustaceans, fish, mollusks, and humans. in humans, it is the leading cause of bacterial seafood-borne gastroenteritis. the focus of this work was to determine the role of alternative sigma factors in the stress response of v. parahaemolyticus rimd2210633, an o3:k6 pandemic isolate. bioinformatics identified five putative extracytoplasmic function (ecf) family of alternative sigma factors: v ...	2014	24935982
detection and quantification of vibrio parahaemolyticus in shellfish from italian production areas.	vibrio parahaemolyticus is a marine microorganism, recognized as an important cause of foodborne illness particularly in asia, south america and united states. outbreaks are rarely reported in europe, but they can occur unexpectedly in relation, among other reasons, to the spread of highly virulent strains. it is known that the risk is proportional to exposure levels to pathogenic v. parahaemolyticus (i.e. carrying the tdh and/or the trh genes) but currently there is a lack of occurrence data fo ...	2014	24810197
plasmid-related quinolone resistance determinants in epidemic vibrio parahaemolyticus, uropathogenic escherichia coli, and marine bacteria from an aquaculture area in chile.	marine bacteria from aquaculture areas with industrial use of quinolones have the potential to pass quinolone resistance genes to animal and human pathogens. the vpa0095 gene, related to the quinolone resistance determinant qnra, from clinical isolates of epidemic vibrio parahaemolyticus conferred reduced susceptibility to quinolone after cloning into escherichia coli k-12 either when acting alone or synergistically with dna gyrase mutations. in addition, a plasmid-mediated quinolone resistance ...	2014	24760167
characterization of putative toxin/antitoxin systems in vibrio parahaemolyticus.	to obtain more information about the toxin/antitoxin (ta) systems in the vibrio genus and also to examine their involvement in the induction of a viable but nonculturable (vbnc) state, we searched homologues of the escherichia coli ta systems in the vibrio parahaemolyticus genome.	2014	24698443
reassessing provider reporting in the age of electronic surveillance.	in 2000, the centers for disease control and prevention began funding health departments to implement integrated electronic systems for disease surveillance.	2014	24458313
vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen.	vibrio parahaemolyticus, an emerging food and waterborne pathogen, is a leading cause of seafood poisoning worldwide. surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interactions with host factors. screening and identification of protective antigens is important for developing therapies against v. parahaemolyticus infections. here, we systematically characterized a novel immunogenic enolase of v. parahaemolyticus. the enolase gene of v. ...	2014	24430205
effect of sypq gene on poly-n-acetylglucosamine biosynthesis in vibrio parahaemolyticus and its role in infection process.	the syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. however, the individual functions of the respective genes remain unclear. amino acid alignment indicates that sypq is presumably involved in biosynthesizing poly-n-acetylglucosamine (pnag), which is proposed to be a critical virulence factor ...	2014	24418930
pvv3, a new shuttle vector for gene expression in vibrio vulnificus.	an efficient electroporation procedure for vibrio vulnificus was designed using the new cloning vector pvv3 (3,107 bp). transformation efficiencies up to 2 × 10(6) transformants per μg dna were achieved. the vector stably replicated in both v. vulnificus and escherichia coli and was also successfully introduced into vibrio parahaemolyticus and vibrio cholerae. to demonstrate the suitability of the vector for molecular cloning, the green fluorescent protein (gfp) gene and the vvhba hemolysin oper ...	2014	24362421
cloning and identification of a novel nhad-type na+/h+ antiporter from metagenomic dna of the halophilic bacteria in soil samples around daban salt lake.	in this study, metagenomic dna was screened for the na(+)/h(+) antiporter gene from the halophilic bacteria in daban salt lake by selection in escherichia coli knabc lacking three major na(+)/h(+) antiporters. one gene designated as hb_nhad encoding a novel nhad-type na(+)/h(+) antiporter was finally cloned. the presence of hb_nhad conferred tolerance of e. coli knabc to up to 0.5 m nacl and 0.2 m licl, and an alkaline ph. hb_nhad has the highest identity (70.6%) with a putative nhad-type na(+)/ ...	2014	24297704
probiotic supplementation influences the diversity of the intestinal microbiota during early stages of farmed senegalese sole (solea senegalensis, kaup 1858).	ingestion of bacteria at early stages results in establishment of a primary intestinal microbiota which likely undergoes several stages along fish life. the role of this intestinal microbiota regulating body functions is crucial for larval development. probiotics have been proved to modulate this microbiota and exert antagonistic effects against fish pathogens. in the present study, we aimed to determine bacterial diversity along different developmental stages of farmed senegalese sole (solea se ...	2014	25103323
antimicrobial effect of bayberry leaf extract for the preservation of large yellow croaker (pseudosciaena crocea).	chemical preservatives have been widely used to keep large yellow croaker fresh. however, the potential harm to human health cannot be ignored. this study was undertaken to investigate the antimicrobial effect of bayberry leaf extract and to evaluate the efficacy of this natural product on the preservation of large yellow croaker.	2014	23929386
genes similar to the vibrio parahaemolyticus virulence-related genes tdh, tlh, and vscc2 occur in other vibrionaceae species isolated from a pristine estuary.	detection of the human pathogen vibrio parahaemolyticus often relies on molecular biological analysis of species-specific virulence factor genes. these genes have been employed in determinations of v. parahaemolyticus population numbers and the prevalence of pathogenic v. parahaemolyticus strains. strains of the vibrionaceae species photobacterium damselae, vibrio diabolicus, vibrio harveyi, and vibrio natriegens, as well as strains similar to vibrio tubiashii, were isolated from a pristine salt ...	2014	24212573
development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals.	rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ...	2014	24954661
a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application.	foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ...	2014	24328501
antibacterial effect of silver nanoparticles against four foodborne pathogens.	there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect.	2014	25147658
vibrio type iii effector vpa1380 is related to the cysteine protease domain of large bacterial toxins.	vibrio parahaemolyticus is a gram-negative halophilic bacterium and one of the leading causes of food-borne gastroenteritis. its genome harbors two type iii secretion systems (t3ss1 and t3ss2), but only t3ss2 is required for enterotoxicity seen in animal models. effector proteins secreted from t3ss2 have been previously shown to promote colonization of the intestinal epithelium, invasion of host cells, and destruction of the epithelial monolayer. in this study, we identify vpa1380, a t3ss2 effec ...	2014	25099122
copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host ampylation on self-assembled human protein microarrays.	ampylation (adenylylation) is a recently discovered mechanism employed by infectious bacteria to regulate host cell signaling. however, despite significant effort, only a few host targets have been identified, limiting our understanding of how these pathogens exploit this mechanism to control host cells. accordingly, we developed a novel nonradioactive ampylation screening platform using high-density cell-free protein microarrays displaying human proteins produced by human translational machiner ...	2014	25073739
an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis.	c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ...	2014	24140432
novel myeloid differentiation factor 88, esmyd88, exhibits estube-binding activity in chinese mitten crab eriocheir sinensis.	myeloid differentiation factor 88 (myd88) is a universal and essential adapter protein that participates in the activation of the toll-like receptor/interleukin-1 receptor-mediated signaling pathway. in the present study, a new myd88 gene (named esmyd88) was identified in the chinese mitten crab eriocheir sinensis. the cdna of esmyd88 was 2210 bp long with a 1416 bp open reading frame that encoded a protein with 472 amino acids. predicted esmyd88 protein had a death domain at the n-terminal and ...	2014	25150191
cloning and characterization of two different l-type lectin genes from the chinese mitten crab eriocheir sinensis.	l-type lectins contain a leguminous lectin domain and bind to high-mannose type oligosaccharides. in the secretory pathway, l-type lectins play crucial functions in the trafficking, sorting, and targeting of maturing glycoproteins. this study identified two novel l-type lectins, designated as esergic-53 and esvip36, from the chinese mitten crab eriocheir sinensis. the complete nucleotide sequence of ergic-53 cdna was 1955 bp, containing a 1506 bp open reading frame (orf) encoding a putative prot ...	2014	24796868
liposome-encapsulated cinnamaldehyde enhances zebrafish (danio rerio) immunity and survival when challenged with vibrio vulnificus and streptococcus agalactiae.	cinnamaldehyde, which is extracted from cinnamon, is a natural compound with activity against bacteria and a modulatory immune function. however, the antibacterial activity and immunostimulation of cinnamaldehyde in fish has not been well investigated due to the compound's poor water solubility. thus, liposome-encapsulated cinnamaldehyde (lec) was used to evaluate the effects of cinnamaldehyde on in vitro antibacterial activity against aquatic pathogens and in vivo immunity and protection parame ...	2014	24632045
molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense.	the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ...	2014	24584659
function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis.	c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ...	2014	24561128
antimicrobial function of the gapdh-related antimicrobial peptide in the skin of skipjack tuna, katsuwonus pelamis.	a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high sequence homology with the yfgap (yellowfin tuna glyceraldehyde-3-phosphate dehydrogenase-related antimicrobial peptide); thus, this peptide was identified as the skip ...	2014	24412436
microbiological quality of seasoned roasted laver and potential hazard control in a real processing line.	microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ...	2014	25474052
enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain).	in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ...	2014	25193866
a multiplex pcr assay for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in korean ready-to-eat food.	a multiplex polymerase chain reaction (pcr) assay was developed for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in various korean ready-to-eat foods. the six specific primer pairs for multiplex pcr were selected based on the o157 antigen (rfbe) gene of e. coli o157:h7, the dna gyrase subunit b (gyrb) gene of b. cereus, the toxin regulatory protein (toxr) gene of v. parahaemolyticu ...	2014	24796416
modelling and mutational analysis of aspergillus nidulans urea, a member of the subfamily of urea/h⁺ transporters in fungi and plants.	we present the first account of the structure-function relationships of a protein of the subfamily of urea/h(+) membrane transporters of fungi and plants, using aspergillus nidulans urea as a study model. based on the crystal structures of the vibrio parahaemolyticus sodium/galactose symporter (vsglt) and of the nucleobase-cation-symport-1 benzylhydantoin transporter from microbacterium liquefaciens (mhp1), we constructed a three-dimensional model of urea which, combined with site-directed and c ...	2014	24966243
antimicrobial function of shβap, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, katsuwonus pelamis.	a 2.3 kda of antimicrobial peptide was purified from an acidified liver extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the amino acid sequence of the purified peptide with those of other known polypeptides revealed high homology with the c-terminus of hemoglobin β-chain; thus, this peptide was designated as the skipjack hemoglobin β chain-related antimicrobial peptide (shβap). shβap showed pot ...	2014	24495783
ptlgbp, a pattern recognition receptor in portunus trituberculatus involved in the immune response against different challenges.	lipopolysaccharide and b-1,3-glucan binding protein (lgbp) is a pattern recognition receptor that can recognize and bind lps and b-1,3-glucan. lgbp has crucial roles in innate immune defense against gram-negative bacteria and fungi. in this study, lgbp functions in portunus trituberculatus innate immunity were analyzed. first, the mrna expression of ptlgbp in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (pamps) stimulations were examined using ...	2014	25194733
identification of a c-type lectin with antiviral and antibacterial activity from pacific white shrimp litopenaeus vannamei.	c-type lectins (ctls) play crucial roles in innate immune responses in invertebrates by recognizing and eliminating microinvaders. in this study, a ctl from pacific white shrimp litopenaeus vannamei (lvctl3) was identified. lvctl3 contains a single c-type lectin-like domain (ctld), which shows similarities to those of other shrimp ctls and has a mutated 'epd' motif in ca(2+)-binding site 2. lvctl3 mrna can be detected in all tested tissues and expression of lvctl3 in gills was up-regulated after ...	2014	24792214
quorum-quenching activity of the ahl-lactonase from bacillus licheniformis dahb1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality.	vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. vibrio virulence is associated with biofilm formation and is regulated by n-acylated homoserine lactone (ahl)-mediated quorum sensing. in an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from indian white shrimps (fenneropenaeus indicus) for an isolate which s ...	2014	25060960
immunological function of familial mediterranean fever disease protein pyrin.	pyrin, encoded by mefv gene, is conserved in humans and mice. mutations in the mefv gene are associated with the human autoinflammatory disease familial mediterranean fever (fmf). pyrin can interact with the inflammasome adaptor asc and induce inflammatory caspase-1 activation in monocytic cells, but the physiological function of pyrin has been unknown for many years. here we summarize previous studies of pyrin function under the context of fmf and immunity, and discuss a recent study demonstrat ...	2014	25307949
innate immune sensing of bacterial modifications of rho gtpases by the pyrin inflammasome.	cytosolic inflammasome complexes mediated by a pattern recognition receptor (prr) defend against pathogen infection by activating caspase 1. pyrin, a candidate prr, can bind to the inflammasome adaptor asc to form a caspase 1-activating complex. mutations in the pyrin-encoding gene, mefv, cause a human autoinflammatory disease known as familial mediterranean fever. despite important roles in immunity and disease, the physiological function of pyrin remains unknown. here we show that pyrin mediat ...	2014	24919149
disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states.	measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ...	2014	24588853
detection of vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae with respect to seasonal fluctuations in temperature and plankton abundance.	over a 1-year period, bi-monthly estuarine surface water and plankton samples (63-200 and > 200 μm fractions) were assayed by polymerase chain reaction for the prevalence of total vibrio parahaemolyticus, v. vulnificus and v. cholerae and select genes associated with clinical strains found in each species. neither temperature nor plankton abundance was a significant correlate of total v. parahaemolyticus; however, the prevalence of genes commonly associated with clinical strains (trh, tdh, orf8) ...	2014	24024909
abundance of vibrio cholerae, v. vulnificus, and v. parahaemolyticus in oysters (crassostrea virginica) and clams (mercenaria mercenaria) from long island sound.	vibriosis is a leading cause of seafood-associated morbidity and mortality in the united states. typically associated with consumption of raw or undercooked oysters, vibriosis associated with clam consumption is increasingly being reported. however, little is known about the prevalence of vibrio spp. in clams. the objective of this study was to compare the levels of vibrio cholerae, vibrio vulnificus, and vibrio parahaemolyticus in oysters and clams harvested concurrently from long island sound ...	2014	25281373
identification of capsule, biofilm, lateral flagellum, and type iv pili in vibrio mimicus strains.	vibrio mimicus is a bacterium that causes gastroenteritis; it is closely related to vibrio cholerae, and can cause acute diarrhea like cholera- or dysentery-type diarrhea. it is distributed worldwide. factors associated with virulence (such as hemolysins, enterotoxins, proteases, phospholipases, aerobactin, and hemagglutinin) have been identified; however, its pathogenicity mechanism is still unknown. in pathogenic vibrio species such as v. cholerae, vibrio. parahaemolyticus and vibrio vulnificu ...	2014	25246027
pathogenic vibrios in environmental, seafood and clinical sources in germany.	bacteria of the family vibrionaceae naturally occur in marine and estuarine environments. only few species of vibrionaceae are associated with human cases of gastroenteritis, ear and wound infections, caused by ingestion of seafood or contact with vibrio containing water. increasing consumption of seafood (fish, fishery products and shellfish) poses a possible source of vibrio infections in germany. additionally, there is a growing concern that abundances of pathogenic vibrios may increase in ge ...	2014	25129553
occurrence of the three major vibrio species pathogenic for human in seafood products consumed in france using real-time pcr.	vibrio spp. have emerged as a serious threat to human health worldwide. vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus are of particular concern as they have been linked to gastrointestinal infections and septicemia associated with the consumption of raw or undercooked seafood. we developed hydrolysis probe-based real-time pcr systems with an internal amplification control for the detection of these species. we applied these systems to a total of 167 fresh or frozen crustacean, f ...	2014	25128747
γ-hydroxynitrile glucosides from the seeds of prinsepia utilis.	γ-hydroxynitrile glucosides (prinsepicyanosides a-e) were isolated alongside 11 known compounds from seeds of prinsepia utilis royle. their structures were determined by detailed analysis of nmr and ms spectroscopic data. the relative configuration of prinsepicyanoside c was established by cu-kα x-ray crystallography. prinsepicyanoside a, osmaronin, and 4-(hydroxylmethyl)-5h-furan-2-one exhibited borderline antibacterial activity against salmonella gallinarum, vibrio parahaemolyticus, and vibrio ...	2014	24952469
high prevalence of toxin producing enteropathogenic vibrios among estuarine crab in ganges delta of west bengal, india.		2014	24927645
sequence and expression divergence of an ancient duplication of the chaperonin groesel operon in vibrio species.	heat-shock proteins are molecular chaperones essential for protein folding, degradation and trafficking. the human pathogen vibrio vulnificus encodes a copy of the groesel operon in both chromosomes and these genes share <80 % similarity with each other. comparative genomic analysis was used to determine whether this duplication is prevalent among vibrionaceae specifically or gammaproteobacteria in general. among the vibrionaceae complete genome sequences in the database (31 species), seven vibr ...	2014	24913685
high-salt preadaptation of vibrio parahaemolyticus enhances survival in response to lethal environmental stresses.	adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. this pathogen is a moderate halophile, with optimal growth at 3% nacl. among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. v. parahaemolyticus has a lysine decarboxylase sys ...	2014	24490918
purification, characterization and production optimization of a vibriocin produced by mangrove associated vibrio parahaemolyticus.	to identify a potential bacterium which produces antimicrobial peptide (vibriocin), and its purification, characterization and production optimization. the bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.	2014	25182547
[vibrio spp. infections of clinical significance and implication for public health].	vibrio spp. infections still are a public health concern. vibrio spp. can be found in marine, estuarine, and freshwater environments, and can be able to cause diseases in fish, shellfish, mammals, as well as in humans. since '80 to date, the number of species within the genus increased from 21 to more than 100. the most important is vibrio cholerae, the etiological agent of the cholera, responsible of seven pandemics; serotypes o1 and o139 can produce cholera toxin, while serotypes non-o1/non-o1 ...	2014	22670341
vibrio parahaemolyticus: a rare cause of chronic diarrhea in a heart transplant patient.	vibrio parahaemolyticus usually causes a self-limiting acute diarrheal illness, and is rarely tested for in cases of chronic diarrhea. we present a rare case of chronic diarrhea caused by v. parahaemolyticus in a heart transplant patient requiring antibiotic treatment.	2014	26157876
incidence of multidrug resistant vibrio parahaemolyticus isolated from ponnani, south india.	the prevalence of vibrio parahaemolyticus has been reported from ponnani earlier, however incidence of multidrug resistant strains have been encountered recently in clinical laboratories. the source for such strains and their presence in this major fish landing centre has been investigated.	2014	25705353
[etiologic characteristics of vibrio parahaemolyticus strains causing outbreaks and sporadic cases in guangdong, 2013].	to study the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of vibrio parahaemolyticus isolated from outbreaks and sporadic cases in guangdong, 2013.	2014	25623459
vibrio parahaemolyticus strengthens their virulence through modulation of cellular reactive oxygen species in vitro.	vibrio parahaemolyticus (vp) is one of the emergent food-borne pathogens that are commensally associated with various shellfish species throughout the world. it is strictly environmental and many strains are pathogenic to humans. the virulent strains cause distinct diseases, including wound infections, septicemia, and most commonly, acute gastroenteritis, which is acquired through the consumption of raw or undercooked seafood, especially shellfish. vp has two type three secretion systems (t3sss) ...	2014	25566508
h-ns is a repressor of major virulence gene loci in vibrio parahaemolyticus.	vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci t3ss1, vp-pai (t3ss1+tdh2) and t6ss2. as showing in this study, the nucleoid-associated dna-binding regulator h-ns binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover h-ns inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of v. parahaemolyticus. h-ns appears to ac ...	2014	25566201
metadynamics simulations reveal a na+ independent exiting path of galactose for the inward-facing conformation of vsglt.	sodium-galactose transporter (sglt) is a secondary active symporter which accumulates sugars into cells by using the electrochemical gradient of na+ across the membrane. previous computational studies provided insights into the release process of the two ligands (galactose and sodium ion) into the cytoplasm from the inward-facing conformation of vibrio parahaemolyticus sodium/galactose transporter (vsglt). several aspects of the transport mechanism of this symporter remain to be clarified: (i) a ...	2014	25522004
comparative genomic analysis of clinical and environmental strains provides insight into the pathogenicity and evolution of vibrio parahaemolyticus.	vibrio parahaemolyticus is a gram-negative halophilic bacterium. infections with the bacterium could become systemic and can be life-threatening to immunocompromised individuals. genome sequences of a few clinical isolates of v. parahaemolyticus are currently available, but the genome dynamics across the species and virulence potential of environmental strains on a genome-scale have not been described before.	2014	25518728
roles of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) in vibrio parahaemolyticus.	vibrio parahaemolyticus is the leading cause of seafood borne bacterial gastroenteritis in the world, often associated with the consumption of raw or undercooked seafood. however, not all strains of v. parahaemolyticus are pathogenic. the thermostable direct hemolysin (tdh) or tdh-related hemolysin (trh) encoded by tdh and trh genes, respectively, are considered major virulence factors in v. parahaemolyticus. however, about 10% of clinical strains do not contain tdh and/or trh. environmental iso ...	2014	25657643
molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab scylla paramamosain.	the thioredoxin (trx) system consists of thioredoxin reductase (trxr), trx, and nicotinamide adenine dinucleotide phosphate (nadph). trxr is an nadph-dependent oxidoreductase. trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. in this study, a cdna sequence (sptrx1) showing high identity to the first trx gene was isolated from a hepatopancreas cdna library of the mud crab scylla paramamosain. the full-le ...	2014	25501236
insights into vibrio parahaemolyticus chn25 response to artificial gastric fluid stress by transcriptomic analysis.	vibrio parahaemolyticus is the causative agent of food-borne gastroenteritis disease. once consumed, human acid gastric fluid is perhaps one of the most important environmental stresses imposed on the bacterium. herein, for the first time, we investigated vibrio parahaemolyticus chn25 response to artificial gastric fluid (agf) stress by transcriptomic analysis. the bacterium at logarithmic growth phase (lgp) displayed lower survival rates than that at stationary growth phase (sgp) under a sub-le ...	2014	25490137
transcriptome and expression profiling analysis of the hemocytes reveals a large number of immune-related genes in mud crab scylla paramamosain during vibrio parahaemolyticus infection.	mud crab scylla paramamosain is an economically important marine species in china. however, frequent outbreaks of infectious diseases caused by marine bacteria, such as vibrio parahaemolyticus, result in great economic losses.	2014	25486443
most-probable-number loop-mediated isothermal amplification-based procedure enhanced with k antigen-specific immunomagnetic separation for quantifying tdh(+) vibrio parahaemolyticus in molluscan shellfish.	although thermostable direct hemolysin-producing (tdh(+)) vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh(+) v. parahaemolyticus remains challenging due to its low densities in the environment. in this study, we developed a most-probable-number (mpn)-based procedure designated a-is(1)-lamp, in which an immunomagnetic separation (ims) technique targeting as many as 69 established k antigens and a loop-mediated isothermal amplification (lamp) a ...	2014	24988012
seasonal abundance of total and pathogenic vibrio parahaemolyticus isolated from american oysters harvested in the mandinga lagoon system, veracruz, mexico: implications for food safety.	the abundance of total and pathogenic vibrio parahaemolyticus (vp) strains in american oysters (crassostrea virginica) harvested in two different harvest sites from the mandinga lagoon system was evaluated monthly for 1 year (january through december 2012). frequencies of species-specific genes and pathogenic genes exhibited a seasonal distribution. the annual occurrence of vp with the species-specific tlh gene (tlh(+)) was significantly higher during the winter windy season (32.50%) and spring ...	2014	24988011
h-ns regulates the vibrio parahaemolyticus type vi secretion system 1.	the marine bacterium vibrio parahaemolyticus, a major cause of food-borne gastroenteritis, employs a type vi secretion system 1 (t6ss1), a recently discovered protein secretion system, to combat competing bacteria. environmental signals such as temperature, salinity, cell density and surface sensing, as well as the quorum-sensing master regulator opar, were previously reported to regulate t6ss1 activity and expression. in this work, we set out to identify additional transcription regulators that ...	2014	24987102
development of a colony hybridization method for the enumeration of total and potentially enteropathogenic vibrio parahaemolyticus in shellfish.	vibrio parahaemolyticus is a marine microorganism, recognized as cause of gastroenteritis outbreaks associated with seafood consumption. in this study the development and the in-house validation of a colony hybridization method for the enumeration of total and potentially pathogenic v. parahaemolyticus is reported. the method included a set of three controls (process, hybridization and detection control) for the full monitoring of the analytical procedure. four digoxigenin-labeled probes were de ...	2014	24984219
occurrence of vibrio parahaemolyticus and vibrio vulnificus in retail raw oysters from the eastern coast of thailand.	occurrence, population density and virulence of vibrio parahaemolyticus and v. vulnificus in 240 retail raw oysters collected monthly between march 2010 and february 2011 from ang sila coast, chon buri province, thailand were determined using most probable number (mpn) multiplex pcr. multiplex pcr detected v. parahaemolyticus in 219 raw oyster samples, of which 29 samples contained the virulence tdh. mpn values for v. parahaemolyticus and pathogenic strains in most samples ranged from 10 to 10(2 ...	2014	24974651
draft genome sequence of trh+ vibrio parahaemolyticus vp-49, isolated from seafood harvested along the mangalore coast, india.	vibrio parahaemolyticus is a seafood-borne pathogen autochthonous to the marine and estuarine ecosystem, which is responsible for gastroenteritis due to the consumption of contaminated raw seafood. here, we report the draft genome sequence of v. parahaemolyticus vp-49, isolated from seafood, to identify the different virulence attributes and to study the mechanisms that enhance its environmental fitness.	2014	24970827