Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| viral pathogens of acute lower respiratory tract infection in china. | to document the viral etiology of acute lower respiratory tract infection (alris) in chinese children. | 2008 | 19129564 |
| [antibiotics prescription in infants hospitalized with respiratory syncytial virus pneumonia]. | although not useful, antibiotics are commonly prescribed in infants with viral pneumonia. | 2008 | 19107304 |
| [viral respiratory tract infections in the first six months of life]. | respiratory syncytial virus and influenza virus infections are known causes of hospital admission in infants. it is less well known the pattern of virus infections in infants under 6 months of age in the outpatient setting. | 2008 | 19128739 |
| risk of respiratory syncytial virus in survivors with severe congenital diaphragmatic hernia. | during the follow-up period in surviving patients with severe congenital diaphragmatic hernia (cdh), respiratory complications, such as recurrence of cdh or chronic lung disease, have been reported to occur as a late complication. although some risk factors for deterioration of respiratory condition have been reported in cdh, the risk of respiratory syncytial virus (rsv) in postoperative cdh patients has not as yet been reported upon. | 2008 | 19143967 |
| [respiratory syncytial virus: as an etiological agent of respiratory tract infection in children and adults]. | respiratory syncytial virus is the most important cause of respiratory tract infection in infants, young children and immunocompromised adults. rsv disease spectrum includes a wide array of respiratory symptoms, from rhinitis and otits media to pneumonia and bronchiolitis. studies have implicated severe rsv infection early in life as a risk factor for subsequent development of reactive airway disease. rsv-infected patients indicated increased levels of th2 cytokines and ige in the patients sera, ... | 2008 | 19209739 |
| ability of low-molecular-weight heparin to alleviate proteinuria by inhibiting respiratory syncytial virus infection. | low-molecular-weight heparin (lmwh) is a negatively charged glycoprotein and has a very similar structure to that of cell surface heparin sulfate (hs). thus, lmwh, an analog of hs, may inhibit positively charged respiratory syncytial virus (rsv) infection through cooperative electrostatic association. | 2008 | 19161362 |
| cytomegalovirus infection and wheezing in infants. | bronchial asthma-like symptoms such as wheezing are commonly associated with respiratory tract infection including respiratory syncytial virus (rsv) infection in infants. no study on the association of wheezing with cytomegalovirus (cmv) infection in infancy has been reported, although cmv infection has been observed to play some role in prolonged and intractable wheezing in limited cases. | 2008 | 19261114 |
| epidemiology of respiratory syncytial virus in various regions within north carolina during multiple seasons. | the centers for disease control and prevention (cdc) monitors the occurrence of respiratory syncytial virus (rsv) in the united states and has historically reported on activity at the regional level. prior to the 2007-2008 rsvseason, the cdc did not report seasonal rsv data for cities within north carolina or for the state. the purpose of the present study is to characterize rsv seasonal activity within north carolina and to determine the appropriate months in which at-risk children should recei ... | 2008 | 19256181 |
| etiology of viral pneumopathies in patients in intensive care unit under mechanical ventilation. | the objective of this work was to define the etiology of viral pneumopathies at the patients from reanimation section being under mechanical ventilation, making reference to viruses with respiratory tropism, and also to chlamydia pneumoniae and mycoplasma pneumoniae. the subjects were 36 patients hospitalized into service of medical reanimation from chu caen and who needed mechanical ventilation more than 48 hours. the samples from the patients were mostly nasal aspirate, 1 bronchial aspirate an ... | 2008 | 19284161 |
| incidence and clinical characteristics of the infection by the respiratory syncytial virus in children admitted in santa casa de são paulo hospital. | the purpose of this study was to identify the rate of infections due to rsv and other viruses in children. in addition we have analyzed demographic data and clinical characteristics of the rsv-positive patients comparing with patients infected by other respiratory viruses. we also described the seasonality of the rsv occurrence in a hospital in são paulo. children below 5 years old admitted in santa casa de são paulo hospital between february 2005 and september 2006 due to acute respiratory infe ... | 2008 | 19287833 |
| effective delivery of igg-antibodies into infected cells via dendritic molecular transporter conjugate iggmt. | igg antibody-transporter conjugates enable intracellular uptake of biologically active igg antibodies that inhibit viral mediated syncytia formation in respiratory syncytial virus green fluorescent protein (rsv-gfp) infected human epithelial cells (hep-2). | 2008 | 19396384 |
| human bocavirus infection in hospitalized children in italy. | human bocavirus (hbov) was first discovered in sweden in 2005 and has now been found worldwide; however its role in clinically relevant diseases has not yet been clearly defined. | 2008 | 19453422 |
| human coronavirus nl63 infections in infants hospitalised with acute respiratory tract infections in south africa. | human coronavirus nl63 (hcov-nl63) is a novel respiratory virus which is associated with respiratory tract infections in children. | 2008 | 19453465 |
| role of influenza and other respiratory viruses in admissions of adults to canadian hospitals. | we sought to estimate age-specific hospitalization rates attributed to influenza and other virus for adults. | 2008 | 19453488 |
| [construction and identification of replication deficient recombinant adenovirus encoding f gene of subgroup a human respiratory syncytial virus]. | a strain of replication deficient recombinant adenvirus encoding fusion glycoprotein (f) of subgroup a human respiratory syncytial virus (rsv) was constructed and the expression of f was identified. | 2008 | 19544635 |
| [in vitro inhibitory mechanism of respiratory syncytial virus with solution prescription of zhidanhuayu]. | to study the effective part of solution prescription of zhidanhuayu (zdhy) against respiratory syncytial virus (rsv) in vitro. | 2008 | 19544654 |
| therapeutic monoclonal antibody treatment targeting respiratory syncytial virus (rsv) g protein mediates viral clearance and reduces the pathogenesis of rsv infection in balb/c mice. | because the g protein of respiratory syncytial virus (rsv) has a cx3c chemokine motif that has been associated with the ability of rsv g protein to modulate the virus-induced host immune response, we examined whether therapeutic treatment with an anti-rsv g monoclonal antibody (mab), 131-2g, that blocks the cx3c-associated activity of rsv g protein might decrease the pulmonary inflammation associated with infection in balb/c mice. the results show that treatment with mab 131-2g on day 3 after rs ... | 2009 | 19545210 |
| use of anionic polymer, poly(methyl vinyl ether-maleic anhydride)-coated beads for capture of respiratory syncytial virus. | respiratory syncytial virus (rsv) is the single most important cause of severe lower respiratory tract infections in infants and young children, and a major public health concern in pediatrics. however, current diagnostic methods for rsv are not sufficiently sensitive. in addition, there is no simple method for enhancing rsv detection. here, a method for capturing rsv from nasal fluid has been developed using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate ... | 2009 | 19546003 |
| rs virus-induced inflammation and the intracellular glutathione redox state in cultured human airway epithelial cells. | there is ample evidence that asthma is mediated by oxidative stress and that viral infection, which is associated with asthma onset and exacerbation in infants, acts as one type of oxidative stress. the goal of this study was to determine whether respiratory syncytial virus (rsv) induces oxidative stress in cultured a549 human airway epithelial cells and normal human bronchial epithelial cells (nhbe), and whether such rsv-induced oxidative stress can induce airway inflammation. to evaluate the d ... | 2009 | 19548075 |
| distribution and seasonality of rhinovirus and other respiratory viruses in a cross-section of asthmatic children in trinidad, west indies. | childhood asthma in the caribbean is advancing in prevalence and morbidity. though viral respiratory tract infections are reported triggers for exacerbations, information on these infections with asthma is sparse in caribbean territories. we examined the distribution of respiratory viruses and their association with seasons in acute and stable asthmatic children in trinidad. | 2009 | 19555507 |
| respiratory viral infections detected by multiplex pcr among pediatric patients with lower respiratory tract infections seen at an urban hospital in delhi from 2005 to 2007. | acute lower respiratory tract infections (alri) are the major cause of morbidity and mortality in young children worldwide. information on viral etiology in alri from india is limited. the aim of the present study was to develop a simple, sensitive, specific and cost effective multiplex pcr (mpcr) assay without post pcr hybridization or nested pcr steps for the detection of respiratory syncytial virus (rsv), influenza viruses, parainfluenza viruses (piv1-3) and human metapneumovirus (hmpv). naso ... | 2009 | 19558656 |
| respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells. | although respiratory syncytial virus (rsv) causes severe lower respiratory tract infection in infants and adults at risk, no rsv vaccine is currently available. in this report, efforts toward the generation of an rsv subunit vaccine using recombinant rsv fusion protein (rrsv-f) are described. the recombinant protein was produced by transient gene expression (tge) in suspension-adapted human embryonic kidney cells (hek-293e) in 4 l orbitally shaken bioreactors. it was then purified and formulated ... | 2009 | 19559121 |
| respiratory pathogens in respiratory tract illnesses during the first year of life: a birth cohort study. | respiratory virus infections are the most important trigger of respiratory illnesses in childhood. data on the occurrence and the clinical impact of respiratory pathogens in the general population of infants are scarce. therefore, we described the occurrence and clinical impact of respiratory pathogens in infants with respiratory tract infections during the first year of life. | 2009 | 19504730 |
| rna interference-mediated silencing of the respiratory syncytial virus nucleocapsid defines a potent antiviral strategy. | we describe the design and characterization of a potent human respiratory syncytial virus (rsv) nucleocapsid gene-specific small interfering rna (sirna), aln-rsv01. in in vitro rsv plaque assays, aln-rsv01 showed a 50% inhibitory concentration of 0.7 nm. sequence analysis of primary isolates of rsv showed that the sirna target site was absolutely conserved in 89/95 isolates, and aln-rsv01 demonstrated activity against all isolates, including those with single-mismatch mutations. in vivo, intrana ... | 2009 | 19506055 |
| secondary rna structure and its role in rna interference to silence the respiratory syncytial virus fusion protein gene. | rna interference (rnai) is a post-transcriptional, gene silencing mechanism which uses small interfering rna molecules (sirna) for gene silencing. respiratory syncytial virus (rsv) is an important respiratory pathogen of medical significance that causes high mortality in infants. the fusion (f) protein of rsv is a good target for therapeutic purposes as it is primarily responsible for penetration of the virus into host cells and subsequent syncytium formation during infection. in the present stu ... | 2009 | 19507066 |
| mesenchymal stem cells over-expressing sdf-1 promote angiogenesis and improve heart function in experimental myocardial infarction in rats. | in addition to its multipotent capability, the mesenchymal stem cell (msc) can secrete and supply a large amount of vascular endothelial growth factor (vegf). the stromal-derived factor-1 alpha (sdf-1alpha) plays an important role in the homing of stem cells to the injured tissues of the heart. therefore, the mscs over-expressing sdf-1alpha could augment the angiogenesis pathway. | 2009 | 19524448 |
| [usefulness of different risk factor associations in predicting admissions due to respiratory syncytial virus in premature newborns of 32 to 35 weeks gestation in spain]. | the aim of the study was to evaluate the risk factor associations for respiratory syncytial virus (rsv) hospitalization in preterm infants from 32 to 35 weeks gestation, treated during two consecutive rsv seasons in spain. | 2009 | 19524492 |
| respiratory syncytial virus infection alters surfactant protein a expression in human pulmonary epithelial cells by reducing translation efficiency. | infection of neonatal lung by respiratory syncytial virus (rsv) is a common cause of respiratory dysfunction. lung alveolar type ii and bronchiolar epithelial (clara) cells secrete surfactant protein a (sp-a), a collectin that is an important component of the pulmonary innate immune system. sp-a binds to the virus, targeting the infectious agent for clearance by host defense mechanisms. we have previously shown that while the steady-state level of sp-a mrna increases approximately threefold afte ... | 2009 | 19525387 |
| application of rt-pcr for diagnosis of respiratory syncytial virus and human metapneumovirus infections in bulgaria, 2006-7 and 2007-8. | we describe here the results of respiratory syncytial virus (rsv) detection by reverse transcription polymerase chain reaction (rt-pcr) during two consecutive seasons, from december 2006 to february 2007 and from october 2007 to march 2008, performed in the national laboratory of influenza and acute respiratory diseases, bulgaria. a total number of 278 nasopharyngeal samples obtained from hospitalised children up to the age of five years were investigated for these two seasons. during the first ... | 2009 | 19531340 |
| antigenic presentation of heterologous epitopes engineered into the outer surface-exposed helix 4 loop region of human papillomavirus l1 capsomeres. | human papillomavirus (hpv) l1 capsid proteins can self-assemble into pentamers (capsomeres) that are immunogenic and can elicit neutralizing antibodies. structural modelling of l1 inter-pentameric interactions predicts that helix 4 (h4) of each of the five l1 monomers project laterally and outwards from the pentamer. we sought to utilize hpv l1 capsomeres as a vaccine platform by engineering heterologous epitopes within l1 derivatives deleted for h4 domain. | 2009 | 19538743 |
| risk factors for respiratory syncytial virus hospitalisation in children with heart disease. | to assess the risk and risk factors for respiratory syncytial virus (rsv) hospitalisation and determinants of the severity of rsv disease in children with heart disease. | 2009 | 19541682 |
| comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients. | sensitive detection of respiratory viruses is important for early diagnosis of infection in patients following hematopoietic cell transplantation (hct). to evaluate the relative sensitivity of respiratory virus detection in specimens from hct recipients, we compared the results of conventional and quantitative molecular methods. | 2009 | 19453994 |
| prevalence of human respiratory viruses in adults with acute respiratory tract infections in beijing, 2005-2007. | to determine the aetiological role and epidemiological profile of common respiratory viruses in adults with acute respiratory tract infections (artis), a 2-year study was conducted in beijing, china, from may 2005 to july 2007. nose and throat swab samples from 5808 arti patients were analysed by pcr methods for common respiratory viruses, including influenza viruses (ifvs) a, b, and c, parainfluenza viruses (pivs) 1-4, enteroviruses (evs), human rhinoviruses (hrvs), respiratory syncytial virus ... | 2009 | 19456830 |
| identification of gene biomarkers for respiratory syncytial virus infection in a bronchial epithelial cell line. | respiratory syncytial virus (rsv) infection involves complex virus-host interplay. in this study, we analyzed gene expression in rsv-infected beas-2b cells to discover novel signaling pathways and biomarkers. we hybridized rnas from rsv- or vehicle-treated beas-2b to affymetrix hu133 plus 2.0 microarrays (n = 4). at 4 and 24 h post-infection, 277 and 900 genes (rsv/control ratio >/=2.0 or </=0.5), and 1 and 12 pathways respectively were significantly altered. twenty-three and 92 genes at 4 and 2 ... | 2009 | 19459069 |
| comparison of the cost of hospitalization for respiratory syncytial virus disease versus palivizumab prophylaxis in canadian inuit infants. | the objectives were to compare actual respiratory syncytial virus (rsv) hospitalization rates and costs in a cohort of inuit infants to hypothetical palivizumab prophylaxis strategies for infants of all gestational ages in the eastern canadian arctic. | 2009 | 19461555 |
| risk-scoring tool for respiratory syncytial virus prophylaxis in premature infants born at 33-35 completed weeks' gestational age in canada. | to study the impact of the risk-scoring tool (rst) as a strategy for targeting prophylaxis effectively in 33-35-week gestational age (ga) canadian infants who range from low to high risk by evaluating the subsequent incidence of respiratory syncytial virus (rsv) infections resulting in emergency room (er) visits and hospitalization. | 2009 | 19469698 |
| [relationship of levels of cd4(+)cd25(+) regulatory t cells and expression of foxp3 mrna in peripheral blood with serum immunoglobulin e level in children with bronchiolitis]. | to study the roles of cd4(+)cd25(+) regulatory t cells and foxp3 mrna in peripheral blood as well as serum total immunoglobulin e (ige) in the pathogenesis of bronchiolitis caused by respiratory syncytial virus (rsv). | 2009 | 19470254 |
| cytokine responses in cord blood predict the severity of later respiratory syncytial virus infection. | it has been claimed that an early respiratory syncytial virus (rsv) infection can induce asthma and recurrent wheezing. | 2009 | 19482350 |
| [respiratory syncytial virus prophylaxis among preterm infants--four seasons' experience]. | respiratory syncitial virus (rsv) is the main reason of hospitalizations due to respiratory tract infection in children within the first year of life. the course of infection is more severe in children from a risk group, which includes children who were born preterm, these with bronchopulmonary dysplasia (bpd), children with heart defects significantly influencing their hemodynamics, and immunocompromised children. palivizumab is a humanized monoclonal antibody class igg-1 used to prevent rsv in ... | 2009 | 19485253 |
| cost-utility analysis of palivizumab in italy: results from a simulation model in the prophylaxis of respiratory syncytial virus infection (rsv) among high-risk preterm infants. | abstract: | 2009 | 19490659 |
| epidemiological assessment of respiratory syncytial virus infection in hospitalized infants, during the season 2005-2006 in palermo, italy. | abstract: | 2009 | 19490666 |
| surveillance for healthcare-acquired febrile respiratory infection in pediatric hospitals participating in the canadian nosocomial infection surveillance program. | to determine the rates of healthcare-acquired febrile respiratory infection (ha-fri) in canadian pediatric hospitals and to determine the vaccination status of patients with healthcare-acquired respiratory syncytial virus (rsv) infection, influenza, or pneumococcal infection who were also eligible for immunoprophylaxis. | 2009 | 19496653 |
| neutrophil tlr4 expression is reduced in the airways of infants with severe bronchiolitis. | in respiratory syncytial virus (rsv) bronchiolitis, neutrophils account for >80% of cells recovered from the airways in bronchoalveolar lavage (bal) fluid. this study investigated neutrophil activation and toll-like receptor (tlr) expression in the blood and lungs of infants with severe rsv bronchiolitis. | 2009 | 19497921 |
| identification of glycosylated sites in rapana hemocyanin by mass spectrometry and gene sequence, and their antiviral effect. | molluscan hemocyanins (hcs) have recently received particular interest due to their significant immunostimulatory properties. this is mainly related to their high carbohydrate content and specific monosaccharide composition. we have now analyzed the oligosaccharides and the carbohydrate linkage sites of the rapana venosa hemocyanin (rvh) using different approaches. we analyzed a number of glycopeptides by lc/esi-ms/ms and identified the sugar chains and peptide sequences of 12 glycopeptides. add ... | 2009 | 19499947 |
| respiratory syncytial virus-induced dysregulation of expression of a mucosal beta-defensin augments colonization of the upper airway by non-typeable haemophilus influenzae. | otitis media (om) is a polymicrobial disease wherein upper respiratory tract viruses compromise host airway defences, which allows bacterial flora of the nasopharynx (np) access to the middle ear. we have shown, in vitro, that respiratory syncytial virus (rsv), a viral co-pathogen of om, reduces transcript abundance of the antimicrobial peptide (ap), chinchilla beta-defensin-1 (cbd-1). here, we demonstrated that chinchillas inoculated with rsv expressed approximately 40% less cbd-1 mrna and prot ... | 2009 | 19500108 |
| rapid detection of respiratory picornaviruses in nasopharyngeal aspirates by immunofluorescence assay. | respiratory picornaviruses (enteroviruses and rhinoviruses) are commonly cited as causes of self-limited upper respiratory tract infection. however, it has recently been suggested that they may cause more severe respiratory disease. immunofluorescence (if) assays are rapid and inexpensive and are often used for the detection of respiratory viruses. | 2009 | 19502108 |
| effect of dexamethasone on respiratory syncytial virus-induced lung inflammation in children: results of a randomized, placebo controlled clinical trial. | inflammatory mediators play a major role in the pathogenesis of respiratory syncytial virus (rsv) infection. the objective of this study was to evaluate the effect of i.v. dexamethasone on cytokine concentrations in tracheal aspirates (ta) of children with severe rsv disease and to correlate them with disease severity. twenty-five cytokines were measured in ta obtained from children <2 yr old intubated for severe rsv disease, and enrolled in a double-blind study of i.v. dexamethasone (0.5 mg/kg; ... | 2009 | 19397752 |
| [respiratory syncytial virus infection promotes the production of thymic stromal lymphopoietin and accelerates th2 inflammation in mouse airway]. | to investigate the effect of respiratory syncytial virus (rsv) infection on the production of thymic stromal lymphopoietin (tslp) and th1/th2 balance in asthmatic mice. | 2009 | 19403406 |
| eleven consecutive years of respiratory syncytial virus outbreaks in croatia. | respiratory syncytial virus (rsv) is the most common cause of severe lower respiratory tract infections (lrti) in infants. the aim of the present study was to analyze the epidemiologic characteristics of rsv outbreaks in croatian children. | 2009 | 19405923 |
| cold oxygen plasma technology efficiency against different airborne respiratory viruses. | respiratory infections caused by viruses are major causes of upper and lower respiratory tract infections. they account for an important mortality and morbidity worldwide. amongst these viruses, influenza viruses and paramyxoviruses are major pathogens. their transmission is mainly airborne, by direct transmission through droplets from infected cases. | 2009 | 19406687 |
| prospective population-based study of rsv-related intermediate care and intensive care unit admissions in switzerland over a 4-year period (2001-2005). | respiratory syncytial virus (rsv) infections are a leading cause of hospital admissions in small children. a substantial proportion of these patients require medical and nursing care, which can only be provided in intermediate (imc) or intensive care units (icu). this article reports on all children aged < 3 years who required admission to imc and/or icu between october 1, 2001 and september 30, 2005 in switzerland. | 2009 | 19412586 |
| infection-related mortality in children with malignancy in england and wales, 2003-2005. | infections are an important component of treatment-related mortality in children with malignancy. infection-related mortality is potentially preventable and it is important to identify areas where prevention and treatment can be improved such as through vaccination, early diagnosis and aggressive management. the aim of this study is to describe the infections contributing to deaths in children under 15 years with malignancy in england and wales in the period 2003-2005. | 2009 | 19415733 |
| recurrent wheezing after respiratory syncytial virus or non-respiratory syncytial virus bronchiolitis in infancy: a 3-year follow-up. | recent studies have suggested that rhinovirus-associated early wheezing is a greater risk factor for development of recurrent wheezing in children than is early wheezing associated with respiratory syncytial virus (rsv). we determined the development of recurrent wheezing in young children within 3 years after hospitalization for rsv or non-rsv bronchiolitis. | 2009 | 19416146 |
| breastfeeding reduces the severity of respiratory syncytial virus infection among young infants: a multi-center prospective study. | the objective of this study was to evaluate the effects of breastfeeding on the severity of respiratory syncytial virus infection in early infancy. | 2009 | 19419530 |
| respiratory syncytial virus-induced activation and migration of respiratory dendritic cells and subsequent antigen presentation in the lung-draining lymph node. | in the respiratory tract, different dendritic cell (dc) populations guard a tight balance between tolerance and immunity to infectious or harmless materials to which the airways are continuously exposed. for infectious and noninfectious antigens administered via different routes, different subsets of dc might contribute during the induction of t-cell tolerance and immunity. we studied the impact of primary respiratory syncytial virus (rsv) infection on respiratory dc composition in c57bl/6 mice. ... | 2009 | 19420085 |
| respiratory syncytial virus proteins modulate suppressors of cytokine signaling 1 and 3 and the type i interferon response to infection by a toll-like receptor pathway. | respiratory syncytial virus (rsv) is a common cause of repeat infections throughout life and potentially severe lower respiratory tract illness in infants, young children, and the elderly. rsv proteins have been shown to contribute to immune evasion by several means, including modification of cytokine and chemokine responses whose expression is negatively regulated by suppressor of cytokine signaling (socs) proteins. in this study, we examine the role of socs1 and socs3 regulation of the type i ... | 2009 | 19435411 |
| anticancer oncolytic activity of respiratory syncytial virus. | oncolytic virotherapy is an emerging biotherapeutic platform for cancer treatment, which is based on selective infection/killing of cancer cells by viruses. herein we identify the human respiratory syncytial virus (rsv) as an oncolytic virus. using prostate cancer models, we show dramatic enhancement of rsv infectivity in vitro in the androgen-independent, highly metastatic pc-3 human prostate cancer cells compared to the non-tumorigenic rwpe-1 human prostate cells. the oncolytic efficiency of r ... | 2009 | 19444304 |
| upregulation of tlr7 and tlr3 gene expression in the lung of respiratory syncytial virus infected mice. | tlr7 and tlr3 (toll-like receptor, tlr) are important pattern recognition receptors (prrs) that recognize the single-strand rna and double-strand rna of virus-origin. respiratory syncytial virus (rsv) could be recognized by both tlr7 and tlr3. we studied the kinetics and profile of lung tlr7 and tlr3 expression during the early phase of the rsv infection, and explored their expression correlation with pulmonary inflammatory response. | 2009 | 19445181 |
| proton-linked dimerization of a retroviral capsid protein initiates capsid assembly. | in mature retroviral particles, the capsid protein (ca) forms a shell encasing the viral replication complex. human immunodeficiency virus (hiv) ca dimerizes in solution, through its c-terminal domain (ctd), and this interaction is important for capsid assembly. in contrast, other retroviral capsid proteins, including that of rous sarcoma virus (rsv), do not dimerize with measurable affinity. here we show, using x-ray crystallography and other biophysical methods, that acidification causes rsv c ... | 2009 | 19446529 |
| heliox reduces respiratory system resistance in respiratory syncytial virus induced respiratory failure. | respiratory syncytial virus (rsv) lower respiratory tract disease is characterised by narrowing of the airways resulting in increased airway resistance, air-trapping and respiratory acidosis. these problems might be overcome using helium-oxygen gas mixture. however, the effect of mechanical ventilation with heliox in these patients is unclear. the objective of this prospective cross-over study was to determine the effects of mechanical ventilation with heliox 60/40 versus conventional gas on res ... | 2009 | 19450268 |
| [impact of a respiratory disease prevention program in high-risk preterm infants: a prospective, multicentric study]. | respiratory tract infections (rti), especially those caused by respiratory syncytial virus (rsv), are of central concern in high-risk preterm infant care. prevention programs including new and costly interventions should be evaluated in terms of effectiveness and impact. | 2009 | 19452082 |
| emerging drugs for respiratory syncytial virus infection. | although respiratory syncytial virus (rsv) was discovered > 40 years ago, treatment remains largely supportive. there are no safe and effective vaccines or specific treatments other than prophylaxis with passive antibody therapy (palivizumab). however, there are good reasons to think that the scene may soon change. as the pace of development of anti-viral drugs accelerates and optimism over vaccines increases, novel therapies are set to make a major impact in the management of this very common i ... | 2009 | 19453286 |
| [viral pathogens of acute lower respiratory tract infection in hospitalized children from east guangdong of china]. | to investigate the viral pathogens of acute lower respiratory tract infection (alrti) in hospitalized children from east guangdong province of china and the relationship of the pathogens with age and seasons. | 2009 | 19292960 |
| the respiratory syncytial virus matrix protein possesses a crm1-mediated nuclear export mechanism. | the respiratory syncytial virus (rsv) matrix (m) protein is localized in the nucleus of infected cells early in infection but is mostly cytoplasmic late in infection. we have previously shown that m localizes in the nucleus through the action of the importin beta1 nuclear import receptor. here, we establish for the first time that m's ability to shuttle to the cytoplasm is due to the action of the nuclear export receptor crm1, as shown in infected cells, and in cells transfected to express green ... | 2009 | 19297465 |
| treating respiratory viral diseases with chemically modified, second generation intranasal sirnas. | chemically synthesized short interfering rna (sirna) of pre-determined sequence has ushered a new era in the application of rna interference (rnai) against viral genes. we have paid particular attention to respiratory viruses that wreak heavy morbidity and mortality worldwide. the clinically significant ones include respiratory syncytial virus (rsv), parainfluenza virus (piv) and influenza virus. as the infection by these viruses is clinically restricted to the respiratory tissues, mainly the lu ... | 2009 | 19301655 |
| nasopharyngeal aspirate cytokine levels 1 yr after severe respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is an important cause of recurrent wheezing in infants. nevertheless, the link between rsv infection and wheezing has yet to be elucidated at the molecular level. here, we present a preliminary study on the evolution of the immune response in the respiratory tract at long-term after rsv infection. twenty-seven immune mediators were profiled in nasopharyngeal aspirates (npas) obtained from 20 children hospitalized due to a severe infection by rsv at dis ... | 2009 | 19302175 |
| 4-methoxycinnamaldehyde inhibited human respiratory syncytial virus in a human larynx carcinoma cell line. | 4-methoxycinnamaldehyde, an active constituent of agastache rugosa, was examined for its cytoprotective activity against rsv by xtt method in human larynx carcinoma cell line. 4-methoxycinnamaldehyde could effectively inhibit cytopathic effect of rsv (p<0.0001) with an estimated ic(50) of 0.055microg/ml and a selectivity index (si) of 898.2. 4-methoxycinnamaldehyde (0.03microg/ml) could inhibit viral entrance by interfering viral attachment (ic(50) of 0.06microg/ml; p<0.0001) and internalization ... | 2009 | 19303275 |
| surfactant protein c-deficient mice are susceptible to respiratory syncytial virus infection. | patients with mutations in the pulmonary surfactant protein c (sp-c) gene develop interstitial lung disease and pulmonary exacerbations associated with viral infections including respiratory syncytial virus (rsv). pulmonary infection with rsv caused more severe interstitial thickening, air space consolidation, and goblet cell hyperplasia in sp-c-deficient (sftpc(-/-)) mice compared with sp-c replete mice. the rsv-induced pathology resolved more slowly in sftpc(-/-) mice with lung inflammation pe ... | 2009 | 19304906 |
| an outbreak of human metapneumovirus infection in hospitalized psychiatric adult patients in taiwan. | human metapneumovirus (hmpv) is a paramyxovirus that is associated with respiratory tract infection (rti) mostly in children, but these outbreaks have rarely been reported in adults. we encountered an outbreak of this disease involving 10 adults in a psychiatric ward in eastern taiwan. the nasopharyngeal swab specimens from 13 patients with symptoms of rti were obtained and analyzed. the rt-pcr tests were negative to influenza virus a/b, adenovirus, rsv, parainfluenza virus, coronavirus, nipah v ... | 2009 | 19308801 |
| bronchiolitis: from empiricism to scientific evidence. | bronchiolitis is the most common viral infection of the lower respiratory tract in infants in their first year of life, with an incidence peak between 3 and 9 months of age. the clinical profile of bronchiolitis results from the inflammatory obstruction of the small airways. the etiological agent involved is respiratory syncytial virus (rsv) in more than 50% of cases. the first international guidelines on the management of children with bronchiolitis have recently been published. the first was p ... | 2009 | 19322125 |
| the host response and molecular pathogenesis associated with respiratory syncytial virus infection. | since the isolation of respiratory syncytial virus (rsv) in 1956, its significance as an important human pathogen in infants, the elderly and the immunocompromised has been established. many important mechanisms contributing to rsv infection, replication and disease pathogenesis have been uncovered; however, there is still insufficient knowledge in these and related areas, which must be addressed to facilitate the development of safe and effective vaccines and therapeutic treatments. a better un ... | 2009 | 19327115 |
| motavizumab, a second-generation humanized mab for the prevention of respiratory syncytial virus infection in high-risk populations. | evolved from palivizumab, motavizumab is a second-generation humanized mab that is in development by medimmune for the prevention of respiratory syncytial virus (rsv) infection in high-risk populations; the drug is also under investigation for the same indication by abbott laboratories. motavizumab targets a highly conserved epitope in the a antigenic site of the rsv fusion (f) protein, which is important in the invasion of rsv from cell to cell. motavizumab, which differs from palivizumab by ju ... | 2009 | 19330726 |
| [use of multiplex pcr method with real-time detection for differential diagnosis of respiratory viral infections]. | multiplex real-time polymerase chain reaction test-system with fluorescent detection (rt-pcr) for simultaneous identification of main agents of acute respiratory viral infections: influenza a (iav) and b viruses (ibv), parainfluenza viruses types 1, 2, 3, 4 (piv 1 - 4), adenoviruses (adv), respiratory syncitial virus (rsv), rhinoviruses (rv) and enteroviruses (ev), in presence internal positive control (ipc) represented by vaccine strain of rubella virus ra 27/3. using multiplex rt-pcr method, r ... | 2009 | 19338239 |
| enhanced delivery and expression of a nanoencapsulated dna vaccine vector for respiratory syncytial virus. | this study evaluated the efficiency of chitosan-encapsulated dna-based respiratory syncytial virus (rsv) vaccine. antigenic regions of rsv f, m2, and g genes were cloned into the human cytomegalovirus promoter-based constitutive expression vector, resulting in a dna vaccine vector named dr-fm2g. this vector was used to formulate dna-chitosan nanoparticles (dcnps) using a complex coacervation process that yielded an encapsulation efficiency of 94.7%. the dcnp sizes ranged from 80 to 150 nm with u ... | 2009 | 19341819 |
| systemic cytokine response profiles associated with respiratory virus-induced acute otitis media. | acute otitis media (aom) results from a complex interplay between the infectious agents and host immune responses. cytokines play a major role in the pathogenesis of aom, but there are few studies on the systemic cytokine response during aom. | 2009 | 19352211 |
| human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment. | human metapneumovirus (hmpv) has been identified as a worldwide agent of serious upper and lower respiratory tract infections in infants and young children. hmpv is second only to respiratory syncytial virus (rsv) as a leading cause of bronchiolitis, and, like rsv, consists of two major genotypes that cocirculate and vary among communities year to year. children who have experienced acute hmpv infection may develop sequelae of wheezing and asthma; however, the features contributing to this patho ... | 2009 | 19357175 |
| [effects of san'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma]. | to evaluate the effects of san'ao decoction (sad) and its analogous prescriptions (aps), compounds of traditional chinese herbal medicine for asthma, on airway inflammation in mice with respiratory syncytial virus (rsv)- and ovalbumin (ova)-induced asthma. | 2009 | 19361366 |
| retroviral capsid assembly: a role for the ca dimer in initiation. | in maturing retroviral virions, ca protein assembles to form a capsid shell that is essential for infectivity. the structure of the two folded domains [n-terminal domain (ntd) and c-terminal domain (ctd)] of ca is highly conserved among various retroviruses, and the capsid assembly pathway, although poorly understood, is thought to be conserved as well. in vitro assembly reactions with purified ca proteins of the rous sarcoma virus (rsv) were used to define factors that influence the kinetics of ... | 2009 | 19361521 |
| humoral immunity to respiratory syncytial virus in young and elderly adults. | respiratory syncytial virus (rsv) has been demonstrated to cause substantial disease in elderly and immunocompromised subjects. the relationship of serum antibody to rsv infection and the risk of infection in elderly subjects is controversial, thus we evaluated the presence of neutralizing antibodies to rsv in healthy people of different age groups and the correlation with viral protection. baseline blood samples from 197 subjects aged 20-80 years were analysed for the presence of anti-rsv antib ... | 2009 | 19366492 |
| adenovirus-mediated thymidine kinase gene therapy induces apoptosis in human epithelial ovarian cancer cells and damages parp-1. | adenoviral (adv) gene therapy with the thymidine kinase gene (tk) under control of the rous sarcoma virus (rsv) promotor followed by the administration of acyclovir leads to replication errors in transcription and to cell death. this concept of adv-rsv-tk has been established for the treatment of ovarian cancer cells. the purpose of this investigation was to clarify whether cell death after adv-rsv-tk gene therapy and acyclovir administration is indeed due to apoptosis induction, whether the syn ... | 2009 | 19368128 |
| genetic evidence for a connection between rous sarcoma virus gag nuclear trafficking and genomic rna packaging. | the packaging of retroviral genomic rna (grna) requires cis-acting elements within the rna and trans-acting elements within the gag polyprotein. the packaging signal psi, at the 5' end of the viral grna, binds to gag through interactions with basic residues and cys-his box rna-binding motifs in the nucleocapsid. although specific interactions between gag and grna have been demonstrated previously, where and when they occur is not well understood. we discovered that the rous sarcoma virus (rsv) g ... | 2009 | 19369339 |
| fosfomycin suppresses chemokine induction in airway epithelial cells infected with respiratory syncytial virus. | respiratory syncytial virus (rsv) infects airway epithelial cells, causing bronchiolitis and pneumonia. inflammation is mediated by various cytokines secreted from rsv-infected airway epithelial cells, and it promotes the pathogenesis of rsv-related diseases. fosfomycin (fof) is approved as a treatment for various bacterial infectious diseases, including respiratory infectious diseases, in japan. fof is suggested to exhibit immunomodulatory effects on lipopolysaccharide-stimulated monocytes and ... | 2009 | 19369476 |
| respiratory viruses and eosinophils: exploring the connections. | in this review, we consider the role played by eosinophilic leukocytes in the pathogenesis and pathophysiology of respiratory virus infection. the vast majority of the available information on this topic focuses on respiratory syncytial virus (rsv; family paramyxoviridae, genus pneumovirus), an important pediatric pathogen that infects infants worldwide. there is no vaccine currently available for rsv. a formalin-inactivated rsv vaccine used in a trial in the 1960s elicited immunopathology in re ... | 2009 | 19375458 |
| seasonality of respiratory viral identification varies with age and aboriginality in metropolitan western australia. | viral respiratory infections are a major cause of pediatric illness. it is not known whether seasonality of viruses differs between aboriginal and non-aboriginal children of varying ages. | 2009 | 19384260 |
| the respiratory syncytial virus m2-1 protein forms tetramers and interacts with rna and p in a competitive manner. | the respiratory syncytial virus (rsv) m2-1 protein is an essential cofactor of the viral rna polymerase complex and functions as a transcriptional processivity and antitermination factor. m2-1, which exists in a phosphorylated or unphosphorylated form in infected cells, is an rna-binding protein that also interacts with some of the other components of the viral polymerase complex. it contains a ccch motif, a putative zinc-binding domain that is essential for m2-1 function, at the n terminus. to ... | 2009 | 19386701 |
| formulation of bovine respiratory syncytial virus fusion protein with cpg oligodeoxynucleotide, cationic host defence peptide and polyphosphazene enhances humoral and cellular responses and induces a protective type 1 immune response in mice. | respiratory syncytial virus (rsv) is the leading cause of serious respiratory tract disease in children and calves; however, rsv vaccine development has been slow due to early observations that formalin-inactivated vaccines induced th2-type immune responses and led to disease enhancement upon subsequent exposure. hence, there is a need for novel adjuvants that will promote a protective th1-type or balanced immune response against rsv. cpg oligodeoxynucleotides (odns), indolicidin, and polyphosph ... | 2009 | 19386785 |
| gene expression of nucleic acid-sensing pattern recognition receptors in children hospitalized for respiratory syncytial virus-associated acute bronchiolitis. | given the critical role of pattern recognition receptors (prrs) in acid nucleic recognition in the initiation of innate immunity and the orchestration of adaptive immunity, the aim of this study was to determine whether any heterogeneity of prr expression in the airway tracts of infants with respiratory syncytial virus (rsv) infection might explain the broad clinical spectrum of rsv-associated bronchiolitis in infants. for this purpose, the levels of melanoma differentiation-associated protein-5 ... | 2009 | 19386802 |
| genetic variability of group a human respiratory syncytial virus strains circulating in germany from 1998 to 2007. | the variability between respiratory syncytial virus (rsv) strains is one of the features of rsv infections that might contribute to the ability of the virus to infect people repeatedly and cause yearly outbreaks. to study the molecular epidemiology of rsv, more than 1,400 rsv isolates from human nasopharyngeal aspirates or nasal or throat swabs from patients with respiratory illness were identified and differentiated by taqman reverse transcription-pcr into groups a and b. rsv group a was domina ... | 2009 | 19386848 |
| bugs and asthma: a different disease? | the prevalence of asthma has dramatically increased in recent decades. exacerbations of asthma are a large contributor to asthma-related costs, and are primarily caused by viral and atypical bacterial infections. rhinoviruses (rvs) are the most common viruses detected after an asthma exacerbation. rvs, respiratory syncytial virus (rsv), and human metapneumovirus (hmpv) viral infections early in life can induce wheezing and are associated with the development of asthma later in life. atypical bac ... | 2009 | 19387028 |
| the effects of disodium cromoglycate on enhanced adherence of haemophilus influenzae to a549 cells infected with respiratory syncytial virus. | nontypeable haemophilus influenzae (nthi) secondary infection often complicates respiratory syncytial virus (rsv) infections. previous studies have revealed that rsv infections enhance nthi adherence to airway epithelial cells. in this study, we investigated the effects of disodium cromoglycate (dscg) and corticosteroids, which are frequently used for the treatment of wheezing often related to rsv infections, on the adherence of nthi to rsv-infected a549 cells. dscg inhibited enhanced adherence ... | 2009 | 19390482 |
| the cotton rat model of respiratory viral infections. | development of successful vaccines against human infectious diseases depends on using appropriate animal models for testing vaccine efficacy and safety. for some viral infections the task is further complicated by the frequently changing genetic make-up of the virus, as in the case of influenza, or by the existence of the little-understood phenomenon of vaccine-enhanced disease, as in the case of respiratory syncytial virus (rsv). the cotton rat sigmodon hispidus has been used for years as an ex ... | 2009 | 19394861 |
| robust and ubiquitous gfp expression in a single generation of chicken embryos using the avian retroviral vector, rcasbp. | functional genomics in avian models has lagged behind that of mammals, and the production of transgenic birds has proven to be challenging and time-consuming. all current methods rely upon breeding chimeric birds through at least one generation. here, we report a rapid method for the ubiquitous expression of gfp in chicken embryos in a single generation (g-0), using the avian retroviral vector, replication-competent avian sarcoma-leukosis virus, with a splice acceptor, bryan rsv pol (rcasbp). hi ... | 2009 | 19395148 |
| exploring the association between severe respiratory syncytial virus infection and asthma: a registry-based twin study. | severe respiratory syncytial virus (rsv) infection is associated with asthma but the nature of this association is imperfectly understood. | 2009 | 19286626 |
| safety, tolerability, pharmacokinetics, and immunogenicity of motavizumab, a humanized, enhanced-potency monoclonal antibody for the prevention of respiratory syncytial virus infection in at-risk children. | : respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young children. motavizumab is an investigational humanized monoclonal antibody for rsv prophylaxis. | 2009 | 19258920 |
| treatment with respiratory syncytial virus g glycoprotein monoclonal antibody or f(ab')2 components mediates reduced pulmonary inflammation in mice. | therapeutic treatment with a non-neutralizing monoclonal antibody (mab) (131-2g) specific to respiratory syncytial virus (rsv) g glycoprotein mediates virus clearance and decreases leukocyte trafficking and interferon gamma (ifn-gamma) production in the lungs of rsv-infected mice. its f(ab')(2) component only mediates decreased leukocyte trafficking and ifn-gamma production without reducing virus replication. thus, this mab has two independent actions that could facilitate treatment and/or preve ... | 2009 | 19264600 |
| genetic diversity and population structure of rice stripe virus in china. | rice stripe virus (rsv) is one of the most economically important pathogens of rice and is repeatedly epidemic in china, japan and korea. the most recent outbreak of rsv in eastern china in 2000 caused significant losses and raised serious concerns. in this paper, we provide a genotyping profile of rsv field isolates and describe the population structure of rsv in china, based on the nucleotide sequences of isolates collected from different geographical regions during 1997-2004. rsv isolates cou ... | 2009 | 19264655 |
| characterization of respiratory syncytial virus m- and m2-specific cd4 t cells in a murine model. | cd4 t cells have been shown to play an important role in the immunity and immunopathogenesis of respiratory syncytial virus (rsv) infection. we identified two novel cd4 t-cell epitopes in the rsv m and m2 proteins with core sequences m(213-223) (fkyikpqsqfi) and m2(27-37) (yfewpphallv). peptides containing the epitopes stimulated rsv-specific cd4 t cells to produce gamma interferon (ifn-gamma), interleukin 2 (il-2), and other th1- and th2-type cytokines in an i-a(b)-restricted pattern. construct ... | 2009 | 19264776 |
| immune dysregulation in severe influenza. | among previously healthy children with severe influenza, the mechanisms leading to increased pathology are not understood. we hypothesized that children with severe influenza would have high levels of circulating cytokines. to examine this, we recruited patients with severe influenza and examined plasma cytokine levels as well as the ability of peripheral blood cells to respond to stimuli. ten patients with severe influenza were enrolled during the 2005-2007 influenza seasons. we evaluated plasm ... | 2009 | 19276177 |
| innate immune signals modulate antiviral and polyreactive antibody responses during severe respiratory syncytial virus infection. | antiviral antibody production during respiratory syncytial virus (rsv) infection in infants is poorly understood. to characterize local b lymphocyte responses, lung tissue and secretions from infants with rsv bronchiolitis were analyzed for innate b cell-stimulating factors and antiviral antibodies. in lung tissues of infants with fatal rsv bronchiolitis, cd20(+) lymphocytes and igm-positive, igg-positive, and iga-positive plasma cells were prominent but cd4(+) t lymphocytes were not. type i int ... | 2009 | 19278337 |
| integrin alphavbeta1 promotes infection by human metapneumovirus. | human metapneumovirus (hmpv) is a recently described paramyxovirus that causes lower respiratory infections in children and adults worldwide. the hmpv fusion (f) protein is a membrane-anchored glycoprotein and major protective antigen. all hmpv f protein sequences determined to date contain an arg-gly-asp (rgd) sequence, suggesting that f engages rgd-binding integrins to mediate cell entry. the divalent cation chelator edta, which disrupts heterodimeric integrin interactions, inhibits infectivit ... | 2009 | 19164533 |