Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the immune response differentially regulates hsp70 and glucocorticoid receptor expression in vitro and in vivo in common carp (cyprinus carpio l.). | heat shock or stress proteins and glucocorticoids (cortisol) regulate a sequential pro-inflammatory and anti-inflammatory cytokine expression profile to effectively kill pathogens, whilst minimizing damage to the host. cortisol elicits its effects through the glucocorticoid receptor (gr) for which hsp70 and hsp90 are required as chaperones. in common carp, (cyprinus carpio) duplicated glucocorticoid receptor genes and splice variants with different cortisol sensitivities exist. we investigated t ... | 2009 | 19061961 |
| identification of methyl triclosan and halogenated analogues in male common carp (cyprinus carpio) from las vegas bay and semipermeable membrane devices from las vegas wash, nevada. | methyl triclosan and four halogenated analogues have been identified in extracts of individual whole-body male carp (cyprinus carpio) tissue that were collected from las vegas bay, nevada, and semipermeable membrane devices (spmd) that were deployed in las vegas wash, nevada. methyl triclosan is believed to be the microbially methylated product of the antibacterial agent triclosan (2, 4, 4'-trichloro-4-hydroxydiphenyl ether, chemical abstract service registry number 3380-34-5, irgasan dp300). th ... | 2009 | 19054547 |
| pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps. | concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. group 1 was maintained for 7 days at 15 degrees c (cold water (cw) group), whereas the second group was subjected to a temperature of 20 degrees c (warm water (ww) group) before sperm sampling. reactivation were achieved after incubation of firstly activated sperm in media with osmotic pressure adjusted up to 300 mo ... | 2009 | 19048380 |
| bioaccumulation of micropollutants and biomarker responses in caged carp (cyprinus carpio). | at four different aquatic sites in flanders (belgium) with different types and degrees of contamination, juvenile carp (cyprinus carpio) were exposed in cages for 4 weeks. after exposure, metals, polychlorinated biphenyls (pcbs), and selected organochlorine pesticides (ocps) were analyzed in the tissues of the carp. besides pollutant accumulation, several effects were measured as well. condition measures such as changes in weight, condition factor (cf), and hepatosomatic index (hsi) were assesse ... | 2009 | 19038452 |
| effect of cadmium chloride on metallothionein levels in carp. | due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. if aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. it is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. in this work the effects of cadmium chloride (2.5, 5, 7.5, 10 and 12.5 mg/l) on common carp (cyprinus carpio) was investigated. we determined cadmium content in tissue of muscle, liver and ... | 2009 | 22408554 |
| glutathione and malondialdehyde levels in common carp after exposure to simazine. | several water contamination incidents with simazine have occurred in the province of badajoz (spain). simazine residues were also detected in drinking water, increasing public health concern. since little information was found concerning the effects of sublethal concentrations of simazine on aquatic organisms, we investigated some oxidative stress biomarkers in tissues of carps from contaminated waters. results confirmed an increase in tissue reduced glutathione and malondialdehyde levels in car ... | 2009 | 21791394 |
| profile of micronucleus frequencies and dna damage in different species of fish in a eutrophic tropical lake. | lake paranoá is a tropical reservoir for the city of brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. at present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. we evaluated both genotoxic and cytotoxic parameters in several fish species (geophagus brasiliensis, cichla temensis, hoplias malabaricus, astyanax bimaculatus lacustres, oreochromis ... | 2009 | 21637659 |
| reduced swimming abilities in fast-growing transgenic common carp cyprinus carpio associated with their morphological variations. | critical swimming speeds (u(crit)) and morphological characters were compared between the f(4) generation of gh-transgenic common carp cyprinus carpio and the non-transgenic controls. transgenic fish displayed a mean absolute u(crit) value 22.3% lower than the controls. principal component analysis identified variations in body shape, with transgenic fish having significantly deeper head, longer caudal length of the dorsal region, longer standard length (l(s)) and shallower body and caudal regio ... | 2009 | 20735532 |
| in vivo inhibition of acetylcholinesterase activity in functionally different tissues of the freshwater fish, cyprinus carpio, under chlorpyrifos exposure. | the inhibitory effect of chlorpyrifos on acetylcholinesterase (ache) activity in different concentrations and exposure periods was investigated in the fish, cyprinus carpio. sublethal concentrations 14% (0.0224 mg/l) and 7% (0.0112 mg/l) of the lethal concentration (0.160 mg/l) of chlorpyrifos were used in the present study. carp were exposed to both toxicant concentrations for 1, 7, and 14 days and were allowed to recover in toxicant-free medium for 7 days after 14 days of exposure. ache activi ... | 2009 | 20408496 |
| regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (cyprinus carpio). | the mechanical properties of the axial muscles vary along the length of a fish's body. this variation in performance correlates with the expression of certain muscle proteins. parvalbumin (parv) is an important calcium binding protein that helps modulate intracellular calcium levels which set the size and shape of the muscle calcium transient. it therefore has a central role in determining the functional properties of the muscle. transcript data revealed eight specific isoforms of parv in common ... | 2009 | 19112137 |
| hepatic and branchial glutathione s-transferases of two fish species: substrate specificity and biotransformation of microcystin-lr. | liver and gills of roach (rutilus rutilus) and silver carp (hypophthalmichthys molitrix) were examined for glutathione s-transferases (gsts) contents and their substrate specificity and capacity to biotransform microcystin-lr (mc-lr). gsts and other glutathione (gsh) affine proteins were purified using a gsh-agarose matrix and separated by anionic chromatography (aec). substrate specificities were determined photometrical for 1-chloro-2,4-dinitrobenzene (cdnb), 1,2-dichloro-4-nitrobenzene (dcnb) ... | 2009 | 19101655 |
| duplication of fgfr1 permits fgf signaling to serve as a target for selection during domestication. | the genetic basis of morphological variation both within and between species has been a lasting question in evolutionary biology and one of considerable recent debate. it is thought that changes in postembryonic development leading to variations in adult form often serve as a basis for selection . thus, we investigated the genetic basis of the development of adult structures in the zebrafish via a forward genetic approach and asked whether the genes and mechanisms found could be predictive of ch ... | 2009 | 19733072 |
| effects of cetyltrimethylammonium chloride on uptake of pyrene by fish gills. | laboratory experiments were carried out to study the effects of cationic surfactant cetyltrimethylammonium chloride (ctac) on the uptake of pyrene by fish gills. the uptake (including adsorption) of pyrene by gills of the color carp (c. carpio var. color) exposed to 20-100 microg/l pyrene in the presence or absence of 0.2-1.0 mg/l ctac were determined. the sorption of pyrene by mucus was preliminary studied using a four-step sequential extraction procedure. fish gills were extracted with h2o, 0. ... | 2009 | 19731836 |
| atlantic cod (gadus morhua) hemoglobin genes: multiplicity and polymorphism. | hemoglobin (hb) polymorphism, assessed by protein gel electrophoresis, has been used almost exclusively to characterize the genetic structure of atlantic cod (gadus morhua) populations and to establish correlations with phenotypic traits such as hb oxygen binding capacity, temperature tolerance and growth characteristics. the genetic system used to explain the results of gel electrophoresis entails the presence of one polymorphic locus with two major alleles (hbi-1; hbi-2). however, vertebrates ... | 2009 | 19728884 |
| expression of connexin 35/36 in retinal horizontal and bipolar cells of carp. | connexin 35/36 (cx35/36) gap junction protein is expressed in various regions of the brain, including the retina. in this work, the expression of cx35/36 in the outer retina of carp was studied by immunocytochemistry. by light microscopy, strong punctate cx35/36-immunoreactivity was observed in the outer plexiform layer. double labeling experiments on vertical retinal sections showed that cx35/36 puncta were localized beneath cone pedicles, stained by recoverin, but not on them. in addition, few ... | 2009 | 19778581 |
| cdna cloning and isolation of somatolactin in mozambique tilapia and effects of seawater acclimation, confinement stress, and fasting on its pituitary expression. | somatolactin (sl) is a member of the growth hormone (gh)/prolactin (prl) family of pituitary hormones, and is found in a variety of teleost species. somatolactin is thought to be involved in a wide range of physiological actions, including reproduction, stress response, the regulation of ca(2+) and acid-base balance, growth, metabolism, and immune response. we report here on the cdna structure of sl from the pituitary of mozambique tilapia, oreochromis mossambicus, and its gene expression in res ... | 2009 | 19133264 |
| effect of a subchronic exposure to simazine on energetic metabolism of common carp (cyprinus carpio). | the energetic parameters, such as glycogen, glucose, proteins, lactate and adenosine triphosphate (atp) content and lactate deshydrogenase (ldh) activity in tissues and blood of carps from simazine (2-chloro-4,6-bis(ethylamino)-s-triazine) contaminated waters were investigated. in order to confirm the field results, a laboratory experiment was designed in which carps were exposed to simazine at the concentration level of 45 micro g. l(- 1) (10-fold of the amount found in natural waters) for 90 d ... | 2009 | 19130373 |
| effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (cyprinus carpio l.). | the aim of this study was to assess the effect of bifenthrin on common carp (cyprinus carpio l.). the 96-h lc50 value of talstar ec 10 (active substance 100 g l(-1) bifenthrin) was found to be 57.5 microg l(-1). examination of haematological and biochemical profiles and histological tissue examination was performed on common carp after 96 h of exposure to talstar ec 10 (57.5 microg l(-1)). the experimental group showed significantly higher (p < 0.01) values of plasma glucose, ammonia, aspartate ... | 2009 | 18766454 |
| the zebrafish stress axis: molecular fallout from the teleost-specific genome duplication event. | the teleost-specific whole genome duplication event 350 million years ago resulted in a variety of duplicated genes that exist in fish today. in this review, we examine whether molecular components involved in the functioning of the hypothalamus-pituitary-interrenal (hpi) axis are present as single or duplicate genes. specifically, we looked at corticotropin releasing hormone (crh), adrenocorticotropic hormone (acth) and the glucocorticoid receptor (gr). the focus is on zebrafish but a variety o ... | 2009 | 18930731 |
| morphine affects the inflammatory response in carp by impairment of leukocyte migration. | opioid peptides are evolutionary conserved and in teleost fish their specific receptor types have been identified not only on neuroendocrine cells but also on immunocytes. in the present work we have studied the effects of morphine, ligand for the mu3 opioid receptor, on innate immune responses of common carp. both in vitro and in vivo, during zymosan-induced peritonitis, morphine reduced gene expression of pro-inflammatory cytokines/chemokines and chemokine receptors. furthermore, in vitro morp ... | 2009 | 18760300 |
| influence of food ration, copper exposure and exercise on the energy metabolism of common carp (cyprinus carpio). | the present study was conducted to extend the understanding of the combined physiological effects of different food rations in combination with sublethal levels of copper in common carp (cyprinus carpio). fish acclimated to low (0.5% body weight) and high (5% body weight) food rations were exposed to 1 microm copper for a period of 28 days and kept for a further 14 days in copper free water to examine their recovery. measurements of oxygen consumption, ammonia excretion and ammonia accumulation ... | 2009 | 18725322 |
| a kinetic model for the relative contribution of waterborne and dietary cadmium and zinc in the common carp (cyprinus carpio). | in this work, the uptake and whole-body accumulation of cd and zn by the common carp from water, diet, and a combination of both was studied using the radioactive tracers 09cd and 65zn. a three-compartment pharmacokinetic model was constructed and revealed metal- and exposure route-dependent accumulation profiles. under the tested exposure conditions in water (0.1 microm cd and 1 microm zn), only 0.1 and 0.07%, respectively, of the total cd and zn load that passed the branchial surfaces was accu ... | 2009 | 18712945 |
| responses of biochemical markers in carp cyprinus carpio from two field sites in western ukraine. | the aim of this study was to compare environmental quality in two sites of the river in western ukraine, rural (r) and industrial (i) during three seasons via a set of biochemical markers in carp cyprinus carpio l. upon comparing the values of the i-site with those of the r-site, we found that mn- and cu, zn-superoxide dismutase (sod) activities decreased and o(.) production increased; metallothionein (mt) and glutathione levels increased in most cases, and acetylcholinesterase (ache) activity d ... | 2009 | 18514900 |
| the complete mitochondrial genome of rock carp procypris rabaudi (cypriniformes: cyprinidae) and phylogenetic implications. | rock carp, procypris rabaudi (tchang), is an endemic fish species in china. we sequenced the complete mitochondrial genome of it by high-fidelity polymerase chain reaction with conserved primers and primer walking sequencing method. the complete mitochondrial genome of rock carp is 16595 bp in length and contains 13 protein-coding genes, two ribosomal rna (rrna) genes, 22 transfer rna (trna) genes and one control region, with an identical order to that of most other vertebrates. the origin of l- ... | 2009 | 18496768 |
| [ecological bases of the combination of natural foci of trematoda infections in the floodplain-river ecosystem of the konda river. communication 2. host population-combined foci of trematoda infections]. | in the context of the present-day teaching of parasitocenoses and the proposition that the pathogen's population is the only compulsory and specific component of a natural focus, the author discloses the ecological bases of the combination of natural foci of opisthorchiasis and methorchiasis (m. bilis), methorchiasis (m. bilis) and methorchiasis (m. xanthosomus). these foci are host population-combined. while analyzing the combination of foci, it is expedient to consider them in pairs since this ... | 2009 | 17436720 |
| [invasion by opisthorchis metacercaria of carp-type fish of the ob in the north of tomsk district]. | 2009 | 4459691 | |
| sphaerospora epinepheli n. sp. (myxosporea: sphaerosporidae) observed in grouper (epinephelus malabaricus). | sphaerospora epinepheli n. sp. is described from grouper, epinephelus malabaricus, in cage-cultured and wild fish collected from both coastal lines of southern thailand. subspherical to spherical spores and mono- or disporous pseudoplasmodia were observed in the lumen of kidney tubules. pseudoplasmodia were round to elongate, size range 15.6-22.9 microns (length) x 8.4-21.6 microns (width). spores were 7.8-10.0 microns (length) x 12.3-14.5 microns (thickness), and 7.0-9.5 microns (width) with tw ... | 2009 | 1920143 |
| [the helminthiasis situation in areas of the influence of water storage basins on the territory of cherkassy province]. | the situation with helminthiases has been studied on the right tributaries of the dnieper, the upper reaches of kremenchug storage pond and in the storage ponds of the south bug basin. the existence of genuine opisthorchiasis foci in kremenchug storage pond is at present impossible due to the absence of codiella mollusks. fish of the carp family is infected in the right tributaries of the dnieper. no diphyllobothriasis foci have been identified in the upper reaches of kremenchug storage pond. op ... | 2009 | 2067500 |
| [development of the trypanosomes (trypanosoma) and cryptobians (cryptobia) of carp and tench in the leech piscicola geometra]. | the paper presents the results of studies of the development of trypanosomes and cryptobiae of carp and tench in piscicola geometra. the paper describes an unknown way of reproduction by means of fusion of individuals and formation of cystlike bodies, the subsequent development of which results in a special form schizogony with a formation of rosettes. | 2009 | 3975069 |
| [hereditary effects of artificial selection of sperm for the endurance trait in the carp cyprinus carpio and the frog rana temporaria]. | studies have been made of the effect of artificial selection of parental sperm by maximum motility time (maximum tolerance) on the body length in carp larva and heat resistance level of muscle tissue in tadpoles. sperm selection increases mean values of these parameters due to the increase in the progeny of the relative number of long-sized individuals and individuals with higher heat resistance of muscle fibers. | 2009 | 3494361 |
| [assisted living: possibilities, limits and architectural requirements--considerations from the viewpoint of an architect with ecopsychological orientation]. | in germany, existing experiences with "sheltered housing" are not sufficient in order to precisely define the role of this new type of housing the aged within the overall network of care for the elderly. first, this article provides an overview of how different the needs for help in old age can be, and which types of supportive environments and services fit to different situations. in this context the potential and the limits of the "sheltered home" concept are described. second, based on some p ... | 2009 | 7871868 |
| [cross reactivity between fish and shellfish]. | in spain, fish allergy represents 18 % of all cases of food allergy in children while reactions caused by crustacea and mollusks account for 3.8 % and 1.6 % respectively. cross-reactivity is defined as the recognition of distinct antigens by the same ige antibody, demonstrable by in vivo and in vitro tests, which clinically manifests as reactions caused by antigens homologous to different species. subclinical sensitization can also occur, giving rise to patients sensitized to particular fish or ... | 2009 | 12783765 |
| koi herpesvirus: distribution and prospects for control in england and wales. | koi herpesvirus (khv) causes a highly virulent disease affecting carp, cyprinus carpio l., and poses a serious socio-economic threat to the uk carp industry. this study aimed to determine the geographic distribution and prevalence of khv exposed fish in england and wales through elisa antibody testing. only three of the 82 farms sampled produced positive results, suggesting fish farms provide a relatively safe source of fish. of the 71 'high-risk' fisheries tested, 26 were positive. all eight ge ... | 2010 | 19878413 |
| sex steroid levels, oocyte maturation and spawning performance in waigieu seaperch (psammoperca waigiensis) exposed to thyroxin, human chorionic gonadotropin, luteinizing hormone releasing hormone and carp pituitary extract. | in the present study, we have investigated the sex steroid hormone levels, oocyte maturation and spawning performance in waigieu seaperch (psammoperca waigiensis) exposed to different doses (0, (control), 0.05, 0.25 and 0.5 mg/kg fish) of thyroxin (t(4)) both through diet (continuously) and injection (single injection). in addition, we also studied plasma steroid hormone levels and spawning performances in female fish injected with a single dose of d-ala(6), pro(9)-net-mgnrh (lhrha: 50 microg/kg ... | 2010 | 19896549 |
| regulation of ovarian steroidogenesis in vitro by igf-i and insulin in common carp, cyprinus carpio: stimulation of aromatase activity and p450arom gene expression. | regulation of ovarian steroidogenesis in vitro by recombinant human insulin-like growth factor-i (igf-i) and bovine insulin (b-insulin) was investigated in intact follicles and isolated follicular cells of carp, cyprinus carpio at vitellogenic stage of oocyte maturation. in intact follicles, igf-i and b-insulin stimulated testosterone and 17beta-estradiol production in vitro. in isolated theca cells, igf-i and b-insulin stimulated testosterone production, whereas in granulosa cells, they stimula ... | 2010 | 19897011 |
| antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (cyprinus carpio koi) and common carp (cyprinus carpio). | the aim of this study was to evaluate antimicrobial susceptibility of aeromonas spp. isolates from common carp and koi carp coming from randomly chosen farms. the isolates were tested for susceptibility to 8 antimicrobial agents using the standard agar dilution susceptibility test. in all isolates, pcr was used to detect the presence of tet(a-e) genes, integrase genes, and gene cassettes. from the total 72 isolates of motile aeromonads sampled from koi carp, 36 isolates (50%) were resistant to o ... | 2010 | 19900770 |
| evaluation of continuous 4-day exposure to peracetic acid as a treatment for ichthyophthirius multifiliis. | the parasitic ciliate ichthyophthirius multifiliis infests all species of freshwater fish and can cause severe economic losses in fish breeding. the most effective treatment, malachite green, has been banned in europe and north america for use in food fish production. peracetic acid (paa) was found to be toxic to i. multifiliis theronts at low concentrations. i. multifiliis-infested carp were exposed to 1 mg/l paa in a dynamic exposure by means of peristaltic pumps. five days after infestation, ... | 2010 | 19902253 |
| mycobacteria isolated from chesapeake bay fish. | mycobacteriosis in fish can result in ulcers, emaciation, and in some cases death. mycobacteria have been previously isolated from a variety of chesapeake bay fish species, and the current study was designed to identify potential host specificity and location fidelity of mycobacterial isolates. mycobacteria were isolated from wild fish of the chesapeake bay collected from the upper bay, the choptank river, herring bay, the chicamacomico river, the pocomoke river and the potomac river in 2003-200 ... | 2010 | 19909394 |
| quantification of cyprinid herpesvirus 3 in environmental water by using an external standard virus. | cyprinid herpesvirus 3 (cyhv-3), a lethal dna virus that spreads in natural lakes and rivers, infects common carp and koi. we established a quantification method for cyhv-3 that includes a viral concentration method and quantitative pcr combined with an external standard virus. viral concentration methods were compared using the cation-coated filter and ultrafiltration methods. the recovery of virus-like particles was similar for the two methods (cation-coated filter method, 44%+/-19%, n=3; ultr ... | 2010 | 19915032 |
| gene cloning, characterization, and heterologous expression of levansucrase from bacillus amyloliquefaciens. | although levan produced by bacillus amyloliquefaciens is known to have efficient immunostimulant property which gives 100% survival of common carp when infected with aeromonas hydrophila, no detailed reports are available describing kinetic studies of d: -glucose production and levan formation. in this study, we cloned and characterized the enzymatic kinetics using levansucrase expressed in escherichia coli. optimum ph for d: -glucose production and levan formation was 6.0 and 8.0, respectively, ... | 2010 | 19916084 |
| effect of oral immunization with aeromonas hydrophila ghosts on protection against experimental fish infection. | to investigate whether oral immunization with aeromonas hydrophila ghosts (ahg) vaccine can elicit mucosal and systemic immune responses of carp (carassius auratus gibelio) compared to conventional formalin-killed bacteria (fkc). | 2010 | 19843213 |
| the genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions. | koi herpesvirus, also known as cyprinid herpesvirus 3 (cyhv-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. cyhv-3 virions present the characteristic morphology of other members of the order herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. this study identified cyhv-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. in additi ... | 2010 | 19846671 |
| experimental transmission of malacosporean parasites from bryozoans to common carp ( cyprinus carpio) and minnow ( phoxinus phoxinus). | to address whether a fish host is involved in the life cycles of malacosporeans of the genus buddenbrockia, cohabitation experiments with different bryozoan and fish species were conducted. samples were analysed by malacosporean-specific pcr, partial sequencing of the 18s rdna, and light and electron microscopy. co-habitation challenges with bryozoans resulted in malacosporean infections detected mainly in the kidney of common carp (cyprinus carpio) and minnow (phoxinus phoxinus). sequences of t ... | 2010 | 19849888 |
| concentration of four heavy metals (cadmium, lead, mercury, and arsenic) in organs of two cyprinid fish (cyprinus carpio and capoeta sp.) from the kor river (iran). | concentration of heavy metals in aquatic animals mainly occurs due to industrial contamination. in this study, the concentrations of four heavy metals (cadmium, lead, mercury, and arsenic) in organs of two cyprinid fish and in water collected from three sections of the kor river, iran were determined using the inductively coupled plasma method. pathological and hormonal changes due to metal contamination were also measured. the concentrations of heavy metals in tissue of fish from the middle sam ... | 2010 | 19711190 |
| intra-individual internal transcribed spacer 1 (its1) and its2 ribosomal sequence variation linked with multiple rdna loci: a case of triploid atractolytocestus huronensis, the monozoic cestode of common carp. | complete sequences of the ribosomal internal transcribed spacers (its1 and its2) and karyological characters of the monozoic (unsegmented) tapeworm atractolytocestus huronensis anthony, 1958 (cestoda: caryophyllidea) from slovakia were analysed, revealing considerable intra-genomic variability and triploidy in all analysed specimens. analysis of 20 sequences of each its1 and its2 spacer yielded eight and 10 different sequence types, respectively. in individual tapeworms, two to four its1 and thr ... | 2010 | 19651130 |
| interstitial deletion of 18q: comparative genomic hybridization array analysis of 46, xx,del(18)(q21.2.q21.33). | interstitial deletion of chromosome 18q is rare, making it difficult to assign phenotypes to particular cytogenetic deletions. | 2010 | 19813260 |
| investigation on the diagnostic sensitivity of molecular tools used for detection of koi herpesvirus. | previous and new pcrs for khv detection were compared by estimation of their sensitivity in recognizing khv dna in plasmids, cell culture extracted khv dna and total dna obtained from field tissue samples. a modified real-time pcr (gilad et al., 2004), combined with an internal control system (ic2, hoffmann et al., 2006) in a duplex assay, was used as a "gold standard". the lowest reliably determined virus concentration between, 5 and 10 khv dna genomic equivalents, was found by real-time pcr (g ... | 2010 | 19819263 |
| enhancement of menadione stress tolerance in yeast by accumulation of hypotaurine and taurine: co-expression of cdna clones, from cyprinus carpio, for cysteine dioxygenase and cysteine sulfinate decarboxylase in saccharomyces cerevisiae. | taurine is known to function as a protectant against various stresses in animal cells. in order to utilize taurine as a compatible solute for stress tolerance of yeast, isolation of cdna clones for genes encoding enzymes involved in biosynthesis of taurine was attempted. two types of cdna clones corresponding to genes encoding cysteine dioxygenase (cdo1 and cdo2) and a cdna clone for cysteine sulfinate decarboxylase (csd) were isolated from cyprinus carpio. deduced amino acid sequences of the tw ... | 2010 | 19633968 |
| methylmercury exposure in a subsistence fishing community in lake chapala, mexico: an ecological approach. | elevated concentrations of mercury have been documented in fish in lake chapala in central mexico, an area that is home to a large subsistence fishing community. however, neither the extent of human mercury exposure nor its sources and routes have been elucidated. | 2010 | 20064246 |
| effects of crude oil and oil fractions on the liver p450-dependent monooxygenase activities and antioxidant defence system of different freshwater fish species. | the effects of crude oil (szeged-algyo, hungary) and oil fractions (f1: rich in aromatics; f2 fraction: free from aromatics) were investigated on liver cyp1a isoenzymes and antioxidant defence system following their i.p. injection into different freshwater fish species: carp (cyprinus carpio l.), silver carp (hyphothalmichtys molitrix v.), and european eel (anquilla anquilla). a dose of 2 ml kg -1 crude oil enhanced erod activity 8-fold in carp and only 5-fold in eel after 3 days. oil fraction f ... | 2010 | 20724273 |
| development and characterization of three new diploid cell lines from labeo rohita (ham.). | development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. three new diploid fish cell lines (rf, rh, and rsb) were developed from fin, heart, and swim bladder of an indian major carp, labeo rohita, commonly called rohu. all the cell lines were optimally maintained at 28 degrees c in leibovitz-15 medium supplemented with 10% fbs. the propagation of rh ... | 2010 | 20730759 |
| inclusion of atlantic salmon in the chinese diet reduces cardiovascular disease risk markers in dyslipidemic adult men. | although the beneficial effects of n-3 fatty acids on several physiologic functions have been widely reported, information about the effects of oily fish in the asian diet on cardiovascular disease (cvd) risk is diminutive. we hypothesize that daily inclusion of oily fish for 8 weeks in the chinese diet will elevate serum eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) levels and reduce cvd risk markers in dyslipidemic adult men, comparable with the effects generally observed by inclu ... | 2010 | 20797476 |
| integrated biological and chemical analysis of organochlorine compound pollution and of its biological effects in a riverine system downstream the discharge point. | pollution in riverine systems, along with its biological effects, may propagate downstream even at considerable distances. we analyzed the organochlorine compound (oc) pollution in a section of the low ebro river (northeast spain) downstream a long-operating chlor-alkali plant. maximal levels of ocs and of their associated dioxin-like biological activity occurred in residue samples from the plant, and persisted in river sediments some 40km downstream (xerta site). biological analysis at multiple ... | 2010 | 20800876 |
| fetuin-a and cystatin c are endogenous inhibitors of human meprin metalloproteases. | meprin α and β, zinc metalloproteinases, play significant roles in inflammation, including inflammatory bowel disease (ibd), possibly by activating cytokines, like interleukin 1β, interleukin 18, or tumor growth factor α. although a number of potential activators for meprins are known, no endogenous inhibitors have been identified. in this work, we analyzed the inhibitory potential of human plasma and identified bovine fetuin-a as an endogenous meprin inhibitor with a k(i) (inhibition constant) ... | 2010 | 20806899 |
| cell cycle and apoptosis regulatory protein (carp)-1 is a novel, adriamycin-inducible, diffuse large b-cell lymphoma (dlbl) growth suppressor. | diffuse large b-cell lymphoma (dlcl) accounts for 30-40% of adult non-hodgkin's lymphoma (nhl). current anti-nhl therapies often target cellular growth suppression pathways and include r-chop (cyclophosphamide, adriamycin, vincristine, and prednisone plus monoclonal anti-cd20 antibody rituximab). however, since many patients relapse, resistant cells to these therapies remain a significant problem and necessitate development of new intervention strategies. cell cycle and apoptosis regulatory prot ... | 2010 | 20809119 |
| important variations in parvalbumin content in common fish species: a factor possibly contributing to variable allergenicity. | although 95% of fish-allergic patients are sensitized to the major fish allergen parvalbumin, clinical reactions to different fish species vary considerably in symptoms, intensity and frequency in allergic subjects. this study aimed at the quantification of parvalbumin levels in salmon, trout, cod, carp, mackerel, herring, redfish and tuna. | 2010 | 20559001 |
| assessing the impact of climate change on disease emergence in freshwater fish in the united kingdom. | a risk framework has been developed to examine the influence of climate change on disease emergence in the united kingdom. the fish immune response and the replication of pathogens are often correlated with water temperature, which manifest as temperature ranges for infection and clinical diseases. these data are reviewed for the major endemic and exotic disease threats to freshwater fish. increasing water temperatures will shift the balance in favour of either the host or pathogen, changing the ... | 2010 | 20561287 |
| uncoupling of bait-protein expression from the prey protein environment adds versatility for cell and tissue interaction proteomics and reveals a complex of carp-1 and the pka cbeta1 subunit. | an expression-uncoupled tandem affinity purification assay is introduced which differs from the standard tap assay by uncoupling the expression of the tap-bait protein from the target cells. here, the tap-tagged bait protein is expressed in escherichia coli and purified. the two concatenated purification steps of the classical tap are performed after addition of the purified bait to brain tissue homogenates, cell and nuclear extracts. without prior genetic manipulation of the target, upscaling, ... | 2010 | 20564261 |
| analysis of gene expression changes associated with long-lasting synaptic enhancement in hippocampal slice cultures after repetitive exposures to glutamate. | we have previously shown that repetitive exposures to glutamate (100 mum, 3 min, three times at 24-hr intervals) induced a long-lasting synaptic enhancement accompanied by synaptogenesis in rat hippocampal slice cultures, a phenomenon termed rise (for repetitive ltp-induced synaptic enhancement). to investigate the molecular mechanisms underlying rise, we first analyzed the time course of gene expression changes between 4 hr and 12 days after repetitive stimulation using an original oligonucleot ... | 2010 | 20568283 |
| a unique feature of toll/il-1 receptor domain-containing adaptor protein is partially responsible for lipopolysaccharide insensitivity in zebrafish with a highly conserved function of myd88. | myd88 and toll/il-1r domain-containing adaptor protein (tirap) are required for the tlr4 response to lps stimulation in mammals, but the functions of the two adaptors and their involvement in zebrafish insensitivity to lps remains unknown. we present a functional analysis of zebrafish myd88 and tirap and suggest that myd88 is more important than tirap for the activation of tlr-mediated nf-kappab, which may be a novel mechanism of myd88-dependent tlr signaling in teleosts. zebrafish tirap lacks t ... | 2010 | 20702732 |
| cloning, characterization, and expression analysis of toll-like receptor-7 cdna from common carp, cyprinus carpio l. | the toll-like receptor 7 (tlr7) is activated by single strand rna and rna-like compounds (imidazoquinoline), and it induces interferon production. we identified and described carp tlr7 cdna and its mrna expression. the full-length cdna of carp tlr7 gene is 3427 bp, encoding 1049 amino acids (ab553573). the similarities of carp tlr7 with zebrafish, rainbow trout, fugu, and human tlr7 were 89.6, 83.4, 80.6 and 74.6%, respectively, at the amino acid sequence level. furthermore, the expression of tl ... | 2010 | 20709611 |
| development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative rt-pcr. | the aim of the present work was to develop a quantitative reverse-transcription polymerase chain reaction (qrt-pcr) assay using a taqman probe to detect and quantify hirame rhabdovirus (hrv). the results demonstrated that the assay had a detection limit of 100 copies of rna per reaction and a log-linear range up to 10(8) copies of hrv rna. regression analysis demonstrated a significant correlation with an r(2) value of 0.9963 and a slope of -3.18 between the mean c(t) values and hrv crna. this a ... | 2010 | 20723563 |
| a new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-κb pathway in skeletal muscle. | a multiprotein complex encompassing a transcription regulator, cardiac ankyrin repeat protein (carp), and the calpain 3 protease was identified in the n2a elastic region of the giant sarcomeric protein titin. the present study aimed to investigate the function(s) of this complex in the skeletal muscle. we demonstrate that carp subcellular localization is controlled by the activity of calpain 3: the higher the calpain 3, the more important the sarcomeric retention of carp. this regulation would o ... | 2010 | 20860623 |
| cxcl8 chemokines in teleost fish: two lineages with distinct expression profiles during early phases of inflammation. | during the inflammatory process, chemokine cxcl8 plays a pivotal role in recruitment of human neutrophilic granulocytes. a diversity of sequences similar to cxcl8 was reported in fish, but their evolutionary relationships and functional homology with their human homolog remain unclear. | 2010 | 20865040 |
| a novel (18)o inverse labeling-based workflow for accurate bottom-up mass spectrometry quantification of proteins separated by gel electrophoresis. | in the present work we report on a novel and fast protocol for accurate bottom-up protein quantification that overcomes the drawbacks of in-gel digestion and maldi analysis, while maintaining their benefits. it relies on the following steps: (i) gel electrophoresis separation of proteins, (ii) fast in-gel protein digestion with trypsin, (iii) (18)o-labeling through the decoupled method, (iv) quantification through selected peptides previously chosen using the (18)o inverse labeling approach and ... | 2010 | 20882554 |
| trypanosomiasis-induced th17-like immune responses in carp. | in mammalian vertebrates, the cytokine interleukin (il)-12 consists of a heterodimer between p35 and p40 subunits whereas interleukin-23 is formed by a heterodimer between p19 and p40 subunits. during an immune response, the balance between il-12 and il-23 can depend on the nature of the pathogen associated molecular pattern (pamp) recognized by, for example tlr2, leading to a preferential production of il-23. il-23 production promotes a th17-mediated immune response characterized by the product ... | 2010 | 20885956 |
| mass mortality associated with koi herpesvirus in wild common carp in canada. | koi herpesvirus (khv) was identified as being associated with more than one mortality event affecting common carp in canada. the first was an extensive mortality event that occurred in 2007 in the kawartha lakes region, ontario, affecting lakes scugog and pigeon. fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. several fish also had branchial columnaris. subsequent mortality events occurred in 2008 in additional bodies of water in south-central ontario ... | 2010 | 20966274 |
| the tissue factor pathway inhibitor 1 of sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection. | tissue factor pathway inhibitor 1 (tfpi-1) is a kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. in teleosts, tfpi-1-like sequences have been found to exist in two species (danio rerio and cyprinus carpio); however, the potential function of fish tfpi-1 has not been investigated. in this study, we identified and analyzed a tfpi-1 homologue, sotfpi-1, from red drum (sciaenops ocellatus). the deduced amino acid sequence of sotfpi-1 is 284 re ... | 2010 | 20970444 |
| prevalence and sequence comparison of phyllodistomum folium from zebra mussel and from freshwater fish in the ebro river. | we utilised dna analysis to detect the presence of the digenean phyllodistomum folium in three cyprinid species, scardinius erythrophthalmus, cyprinus carpio and rutilus rutilus. dna sequencing of the region containing the genes its1-5.8s-its2 revealed 100% sequence identity between dna from the sporocysts found in zebra mussels and dna from adults located in the urinary system of 29 cyprinid fish. a second genetically different (variation=1.6%) sequence was observed in two samples from r. rutil ... | 2010 | 20971214 |
| derivation and characterization of embryonic stem-like cells of indian major carp catla catla. | embryonic stem (es)-like cells were derived from mid-blastula stage embryos of a freshwater fish, catla catla catla, under feeder-free condition and designated as cces cells. the conditioned media was optimized with 10% foetal bovine serum (fbs), fish embryo extract (fee) having 100 µg ml(-1) protein concentration, 15 ng ml(-1) basic fibroblast growth factor (bfgf) and basic media containing leibovitz-15, dmem with 4·5 g l(-1) glucose and ham's f12 (ldf) in 2:1:1 ratio using a primary culture of ... | 2010 | 21039493 |
| identification of calcium-binding proteins in fish seminal plasma. | calcium ions play an important role in the activation of fish sperm movement. the mechanism of their binding in semen is still unknown. the goal of this study was the development of a method for identifying calcium-binding proteins in fish seminal plasma. two methods of calcium-binding proteins detection were tested with the use of quin2 and stains-all dyes. the first method was useful for the identification of calcium-binding proteins of fish seminal plasma. it consisted of proteins separation ... | 2010 | 21042848 |
| expression of immune-related genes in rohu labeo rohita (hamilton) by experimental freshwater lice argulus siamensis (wilson) infection. | the crustacean ectoparasite, argulus poses one of the major threats to carp culture due to absence of any suitable control measure. the study was undertaken to determine the expression of immune-related genes in three major immunocompetent organs viz., kidney, skin and liver of rohu (labeo rohita) during experimental freshwater lice argulus siamensis infection. results showed that the expression of tlr 22-like, lysozyme g and β2-microglobulin genes in kidney was significantly (p ≤ 0.05) down-reg ... | 2010 | 21051150 |
| biologic responses of bacteria communities living at the mucus secretion of common carp (cyprinus carpio) after exposure to the carbon nanomaterial fullerene (c(60)). | bacteria communities living in mucus secretions of common carp cyprinus carpio (cyprinidae) were exposed to the organic nanomaterial fullerene (c(60)) to evaluate its potential bactericidal effects. end points analyzed were viability, growth, reactive oxygen species (ros) concentration, and total antioxidant competence against peroxyl radicals. viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of c(60) assayed (0.1, ... | 2010 | 21072630 |
| toxicological responses of cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. | cyprinus carpio was exposed to imazethapyr and imazapic at laboratory and at field conditions. the laboratory experiment was carried out for 7 days and at rice field for 7, 30 and 90 days. oxidative stress parameters and antioxidant profile were studied as well as metabolic parameters. after 7 days, brain ache activity increases in laboratory and field, but in muscle, reduction was observed only in laboratory. at the same period, brain and muscle tbars and liver cat increase in the laboratory. m ... | 2010 | 21095003 |
| the rapid generation of chimerical genes expanding protein diversity in zebrafish. | variation of gene number among species indicates that there is a general process of new gene origination. one of the major mechanism providing raw materials for the origin of new genes is gene duplication. retroposition, as a special type of gene duplication- the rna-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome. | 2010 | 21106061 |
| differential responses to natural and recombinant allergens in a murine model of fish allergy. | aerosolized fish proteins are an important cause of allergic airway reactions in both the domestic and the occupational environment. the aim of this study was to investigate inhalant fish-induced allergy in a mouse model and compare immune responses generated by raw and heat-treated fish extracts as well as natural and recombinant forms of the major fish allergen parvalbumin. mice were sensitized with raw or cooked pilchard extract and challenged intranasally with cooked pilchard extract, purifi ... | 2010 | 21112638 |
| cyprinid herpesvirus 3. | the recently designated cyprinid herpesvirus 3 (cyhv-3) is an emerging agent that causes fatal disease in common and koi carp. since its emergence in the late 1990s, this highly contagious pathogen has caused severe financial losses in common and koi carp culture industries worldwide. in addition to its economic role, recent studies suggest that cyhv-3 may have a role in fundamental research. cyhv-3 has the largest genome among viruses in the order herpesvirales and serves as a model for mutagen ... | 2010 | 21122210 |
| hippocampal carp over-expression solidifies consolidation of contextual fear memories. | the doublecortin-like kinase (dclk) gene is involved in neuronal migration during development. through alternative splicing the dclk gene also produces a transcript called ca(2+)/calmodulin dependent protein kinase (camk)-related peptide (carp) that is expressed exclusively during adulthood in response to neuronal activity. the function of carp, however, is poorly understood. to study carp function, we have generated transgenic mice with over-expression of the carp transcript in, amongst other b ... | 2010 | 21130104 |
| activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268. | carp muscle-specific creatine kinase m1 isoenzyme (m1-ck) seems to have evolved to adapt to synchronized changes in body temperature and intracellular ph. when gly(268) in rabbit muscle-specific creatine kinase was substituted with asn(268) as found in carp m1-ck, the rabbit muscle-specific ck g286n mutant specific activity at ph 8.0 and 10°c was more than 2-fold higher than that in the wild-type rabbit enzyme. kinetic studies showed that k(m) values of the rabbit ck g268n mutant were similar to ... | 2010 | 21130895 |
| the ontogeny of mauthner cells in the brain of labeo rohita as revealed by nadph-d and nnos immunohistochemistry. | nitric oxide (no) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. we are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) and neuronal nitric oxide synthase (nnos) in the mauthner cells (mc) of the carp, labeo rohita brain during post-embryonic development. the nadph-d and nnos-positive mc were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. in adult, these ce ... | 2010 | 21132510 |
| the application of hcg, cph and ovopel in successful artificial reproduction of goldfish (carassius auratus auratus) under controlled conditions. | artificial reproduction of fish is one of the main goals of aquaculture production. the aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (cph), ovopel and human chorionic gonadotropin (hcg), administered as a one-off dose and inducing two spawns in the same fish within a short time period. goldfish spawners were stimulated with hcg, cph and ovopel, and the results were compared to the fish ... | 2010 | 21134006 |
| generation of monoclonal antibodies specific for orf68 of koi herpesvirus. | outbreaks of koi herpesvirus (khv) infection in carp are still a serious problem worldwide. khv is closely related to other two cyprinid herpesviruses, pox herpesvirus (chv) and haematopoietic necrosis herpesvirus (cyhv-2) in goldfish. in this study, two major khv antigenic proteins (orf62 and orf68) were identified by immunoscreening using a khv-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. after screening hybridoma cells, one mab agai ... | 2010 | 21134694 |
| [study on genotoxicity of aldicarb and methomyl]. | genotoxicity of aldicarb and methomyl was explored. the aldicarb and methomyl were diluted by the deionized water respectively, and then five concentrations of aldicarb were generated as 0.002, 0.02, 0.2, 2, 20 microg/l, methomyl as 0.02, 0.2, 2, 20, 200 microg/l. the micronucleus of carp erythrocyte was counted by micronucleus test. the mutation of bacteria was assessed by ames test. the dna damage of human lymphocytes was tested by comet assay. the genotoxicity of aldicarb and methomyl was est ... | 2010 | 21360888 |
| comparative susceptibility of carp fingerlings to lernaea cyprinacea infection. | study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (labeo fimbriatus, l. rohita, l. calbasu, catla catla, ctenopharyngodon idella, cyprinus carpio and hypophthamichthys molitrix) grown under both mono and polyculture to lernaea cyprinacea infection. under monoculture, c. carpio, l. rohita and l. calbasu, did not acquire lernaea infection and were thus considered resistant, whereas c. idella, h. molitrix, c. catla and l. fimbriatus were suscept ... | 2010 | 21255932 |
| the individual and combined effects of deoxynivalenol and aflatoxin b(1) on primary hepatocytes of cyprinus carpio. | aflatoxin b(1) (afb(1)) and deoxynivalenol (don) are important food-borne mycotoxins that have been implicated in animal and human health. in this study, individual and combinative effects of afb(1) and don were tested in primary hepatocytes of cyprinus carpio. the results indicated that the combinative effects of afb(1) and don (0.01 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.5 μg/ml don) were higher than that of individual mycotoxin (p < 0.05 ... | 2010 | 21152299 |
| isolation and characterization of koi herpesvirus (khv) from indonesia: identification of a new genetic lineage. | koi herpesvirus (khv) is the aetiological agent of an emerging disease (khvd) associated with mass mortalities in koi and common carp and reported from at least 30 countries. we report the first isolation of khv from koi and common carp in indonesia and initial characterization of the isolates. clinical signs, histopathology and virion morphology are similar to those of isolates from other countries. phylogenetic analyses using the thymidine kinase gene amplified from each isolate and from carp ... | 2010 | 21158870 |
| haematological indices are modulated in juvenile carp, cyprinus carpio l., exposed to microcystins produced by cyanobacterial water bloom. | this study evaluated the influence of toxic cyanobacterial water blooms on the blood indices of the common carp, cyprinus carpio l. experimental fish were exposed to a natural population of cyanobacterial water blooms (mainly microcystis aeruginosa and m. ichthyoblabe), which contained microcystins [total concentration 133-284 μg g⁻¹ (dw), concentration in water 2.8-7.4 μg l⁻¹]. haematological indices showed marked changes in fish exposed to the cyanobacterial population in comparison with the c ... | 2010 | 21158871 |
| potential use of probiotic- and triherbal extract-enriched diets to control aeromonas hydrophila infection in carp. | this study reports the effect of probiotic- and triherbal extract-enriched diet on cyprinus carpio (52 +/- 2 g, n = 250) inoculated intramuscularly (50 microl) with aeromonas hydrophila (1.8 x 106 cfu ml(-1)). on day 6 post-infection, the fish were divided into 4 groups and fed with the basal diet (iu) or with diets supplemented with triherbal extract (h), sporolac (s) or lactobacillus (l). a fifth group of non-infected fish fed with the basal diet was used as a control. haematology and immunolo ... | 2010 | 21166313 |
| [correlation and location of est markers with cold tolerance trait of common carp (cyprinus carpio l)]. | expressed sequence tags (ests) are parts of complementary dnas (cdnas) with certain gene function.it provides us more information than other neutral markers. the association of est markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. in this study, 12 candidate est markers isolated from the cold-induced brain cdna of common carp (cyprinus carpio l.) were conducted the correlation analysis of marker and cold tol ... | 2010 | 21174346 |
| nitrosative stress during infection-induced inflammation in fish: lessons from a host-parasite infection model. | the inflammatory response should be considered a protective immune reaction of the host aimed at the removal of pathogens, sometimes irrespective of negative side-effects. in this review we discuss the differential contribution of macrophages and neutrophilic granulocytes to nitrosative stress in vivo and discuss how the timing and concentration of nitric oxide (no·) are important factors determining the degree of nitrosative stress during parasite-induced inflammation. infections of common carp ... | 2010 | 21184662 |
| genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional orf that could be expressed in fish cell. | genomic sequence of scophthalmus maximus rhabdovirus (smrv) isolated from diseased turbot has been characterized. the complete genome of smrv comprises 11,492 nucleotides and encodes five typical rhabdovirus genes n, p, m, g and l. in addition, two open reading frames (orf) are predicted overlapping with p gene, one upstream of p and smaller than p (temporarily called ps), and another in p gene which may encodes a protein similar to the vesicular stomatitis virus c protein. the c orf is containe ... | 2010 | 21185339 |
| oxidative stress biomarkers in cyprinus carpio exposed to commercial herbicide bispyribac-sodium. | cyprinus carpio were exposed under field conditions to 20.87 microg l(-1) of commercial herbicide bispyribac-sodium (nominee, sc), during 7, 21 and 72 days. enzymatic parameters such as catalase (cat), glutathione s-transferase (gst) and acetylcholinesterase (ache) activities, as well as thiobarbituric acid-reactive substances (tbars) and protein carbonyl contents were studied in different tissues. after 7 days of exposure, gst activity decreased. at the same period, brain ache activity increase ... | 2010 | 20809548 |
| toxicity of organic pollutants to seven aquatic organisms: effect of polarity and ionization. | the toxicity of organic chemicals to vibrio fischeri, river bacteria, algae, daphnia magna and fishes were analysed. the results showed that the toxicity of chemicals to narcotics was dependent on hydrophobicity. a single model for both polar and non-polar narcotics was developed by inclusion of a polarity descriptor as well as the hydrophobic parameter. the highly hydrophobic polar narcotics could be treated as non-polar narcotics because their polar functional group(s) make(s) a relatively sma ... | 2010 | 20818578 |
| immunomodulatory effect of aegle marmelos leaf extract on freshwater fish cyprinus carpio infected by bacterial pathogen aeromonas hydrophila. | aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. the use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity. | 2010 | 20818950 |
| carbonic anhydrase related protein viii and its role in neurodegeneration and cancer. | mammalian carbonic anhydrase (α-ca) gene family comprises sixteen isoforms, thirteen of which are active isozymes and three isoforms lack classical ca activity of reversible hydration of co(2) due to absence of one or more histidine residues required for ca catalytic activity. the inactive isoforms are known as carbonic anhydrase related proteins (carps) viii, x and xi. among these three, carp viii was reported first in 1990 from a mouse brain cdna library and is well studied structurally as wel ... | 2010 | 20819067 |
| hemolysin etha in edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system esra-esrb and nucleoid protein hhaet. | edwardsiella tarda is a gram-negative pathogen for hemorrhagic septicemia in fish. recently, two-component system (tcs) esra-esrb in e. tarda has been found to play key roles in regulating type iii secretion system (ttss) and type vi secretion system (t6ss). in this study, a markedly attenuated δesrb mutant was investigated to exhibit enhanced cell-invasion capability, as well as the increased cytotoxicity of its extracellular products (ecps). compared with the parental strain, the δesrb mutant ... | 2010 | 20832475 |
| variability of responses in the crucian carp carassius carassius from two ukrainian ponds determined by multi-marker approach. | the aim of this study was to evaluate the health status of the comparatively tolerant fish carassius carassius over three seasons in an area characterized by spontaneous human activities. sites near the springs of a river (site z) and downstream of a river (site b) in western ukraine were selected. according to the centroid grouping analysis, the biochemical and morphological indices allowed the fish to be distinguished according to season more than to site. the level of nuclear abnormalities wa ... | 2010 | 20832861 |
| novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species. | various polyclonal and monoclonal antibodies have been developed for vitellogenin (vtg) bioassays in different aquatic species. preparation of these reagents is time-consuming and expensive. in the present study, a phage-displayed, recombinant, single-chain variable fragment (scfv) format antibody library was constructed using splenic mrna from non-immunized mice. after 3 rounds of panning, 3 scfv antibodies with specificity for the highly conserved n-terminal region of cyprinid fish vtg were is ... | 2010 | 21290899 |
| changes in skin mucus of common carp, cyprinus carpio l., after exposure to water with a high bacterial load. | water in aquaculture systems may contain a high load of microorganisms. reduction in overall bacterial tank water load improves fish health and growth parameters. in this study, the effect of an increase of overall bacterial load in tank water on carp skin mucus was assessed. intracellular and released high molecular weight glycoproteins (hmgs) of carp skin mucus were analysed for changes using histological, histochemical and biochemical techniques. increase of bacterial load did not induce obvi ... | 2010 | 20298445 |
| behavioral and morphological endpoints: as an early response to sublethal malathion intoxication in the freshwater fish, labeo rohita. | a short-term definitive test by the static renewal bioassay method was conducted to determine the acute toxicity (lc(50)) of commercial-grade organophosphate insecticide, malathion (50% ec) on the freshwater fish, labeo rohita. carp fingerlings were exposed to different concentrations of malathion for 96 hours. the acute toxicity (lc(50)) of malathion was found to be 4.5 microg/l. one tenth (1/10, 0.45 microg/l) of the acute toxicity value was selected as the sublethal concentration for subacute ... | 2010 | 20307142 |