Publications

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response of aedes aegypti (diptera: culicidae) larvae to three xenobiotic exposures: larval tolerance and detoxifying enzyme activities.the ability of mosquito larvae to tolerate toxic compounds (temephos, bacillus thuringiensis var. israelensis, toxic vegetable leaf litter) was examined on a laboratory larval strain of aedes aegypti l. bioassays and detoxifying enzyme activity measurements were performed to compare tolerance/resistance capacities. the possibility of a functional plasticity of detoxifying equipment was investigated through experimental determination of the inductive effect of each xenobiotic within a given gener ...200616519308
a new clothing impregnation method for personal protection against ticks and biting insects.the efficacy and residual activity of a factory-based, permethrin-impregnated military battle dress uniform (bdu) using a new polymer-coating technique has been evaluated by laboratory and field testing during deployment to afghanistan and compared with two commercially available, widely used dipping methods. residual permethrin concentrations and remaining contact toxicities on treated fabrics before laundering, after up to 100 launderings as well as after being worn-out during deployment were ...200616524779
repellency of essential oils extracted from plants in thailand against four mosquito vectors (diptera: culicidae) and oviposition deterrent effects against aedes aegypti (diptera: culicidae).in this study we evaluated and reported repellent effects of essential oils from thai plants against 4 mosquito vectors: aedes aegypti, ae. albopictus, anopheles. dirus and culex quinquefasciatus under laboratory conditions using human volunteers. the essential oils were extracted from 18 plant species, belonging to 11 families, and the oils were then prepared as 10% solution in absolute ethanol with additives. two chemical repellents, deet and ir3535, were also prepared in the same formulation ...200617333734
climatic factors associated with epidemic dengue in palembang, indonesia: implications of short-term meteorological events on virus transmission.an extensive outbreak of dengue fever and dengue hemorhagic fever occurred in the city of palembang, south sumatra, indonesia from late 1997 through march/april 1998. all surveyed administrative areas (kelurahan) in palembang were found to be 'permissive' for dengue virus transmission; and all areas that had aedes (subgenus stegomyia) larval mosquitoes in abundance experienced increased cases of dhf during the epidemic. the aedes house index (hi) for combined aedes aegypti and aedes albopictus w ...200617333762
upscale or downscale: applications of fine scale remotely sensed data to chagas disease in argentina and schistosomiasis in kenya.depending on the research question or the public health application, the appropriate resolution of the data varies temporally, spatially, and, for satellite data, spectrally and radiometrically. regardless of the scale used to address a research or public health question, the temptation is always there to extrapolate from fine-resolution data or to interpolate from coarse resolution studies. in both cases, the relevance of data and analyses conducted on one spatial level to other levels cannot b ...200617476311
vector competence of aedes aegypti (l.) and culex quinquefasciatus (say) for dirofilaria immitis (leidy).this study was performed to examine the vector competence of aedes aegypti and culex quinquefasciatus for dirofilaria immitis. eleven individual experiments were conducted in this study. nonthaburi and udon thani strains of ae. aegypti were allowed to feed on infected dogs that had 5,750 and 4,600 microfilariae (mw) per ml of blood, respectively. three groups of bangkok-strain cx. quinquefasciatus were allowed to feed on dogs that had 4,800, 5,200, and 5,850 mf per ml of blood. six groups of liv ...200617547063
models of dengue virus infection.the need for models of dengue disease has reached a pinnacle as the transmission of this mosquito-borne virus has increased dramatically. little is known about the mechanisms that lead to dengue fever and its more severe form, dengue hemorrhagic fever; this is owing to the fact that only humans show signs of disease. in the past 5 years, research has better identified the initial target cells of infection, and this has led to the development of models of infection in primary human cell cultures. ...200618087566
[population dynamics of the immature stages of aedes aegypti (diptera: culicidae), vector of dengue: a longitudinal study (1996-2000)].a four year study was conducted on a natural population of immature stages of aedes aegypti after the re-invasion of argentina by this vector in 1987. thirty six plastic containers with 700 ml of dechlorinated water were placed in the la plata zoological garden, la plata, argentina. a strip of filter paper around each container was added to facilitate egg counting. eggs, larvae and pupae were counted weekly in each container from september, 1996 to august, 2000. after egg counting, papers were s ...200618491640
differential induction of proteins in anopheles gambiae sensu stricto (diptera: cullicidae) larvae in response to heavy metal selection.investigations were conducted to establish the magnitude and pattern of differential expression of proteins due to generational selection of third instar an. gambiae s.s. larvae by cadmium, copper and lead heavy metals, three possible common urban pollutants.a susceptible strain of an. gambiae s.s. third instar larvae was separately placed under selection pressure with cadmium, copper and lead at lc(30) and controls through five generations. first, third and fifth generation selection survivors ...200620651951
functional role of aspartic proteinase cathepsin d in insect metamorphosis.metamorphosis is a complex, highly conserved and strictly regulated development process that involves the programmed cell death of obsolete larval organs. here we show a novel functional role for the aspartic proteinase cathepsin d during insect metamorphosis.200617062167
asap: a resource for annotating, curating, comparing, and disseminating genomic data.asap is a comprehensive web-based system for community genome annotation and analysis. asap is being used for a large-scale effort to augment and curate annotations for genomes of enterobacterial pathogens and for additional genome sequences. new tools, such as the genome alignment program mauve, have been incorporated into asap in order to improve display and analysis of related genomes. recent improvements to the database and challenges for future development of the system are discussed. asap ...200616381899
adult longevity of certain mosquito species after larval and pupal exposure to sublethal concentration of an insect growth regulator, hexaflumuron.longevity of the adults of three vector species, culex quinquefasciatus, anopheles stephensi, and aedes aegypti was drastically reduced when they were exposed at larval and pupal stages to sublethal concentrations of an insect growth regulator hexaflumuron. when the three species were exposed to 0.05 mg/l at the pupal stage, males and females of cx. quinquefasciatus suffered a more shortened life span than other species. among the females whose feeding activity was adversely affected by igr trea ...20061523463
the tryptophan oxidation pathway in mosquitoes with emphasis on xanthurenic acid biosynthesis.oxidation of tryptophan to kynurenine and 3-hydroxykynurenine (3-hk) is the major catabolic pathway in mosquitoes. however, 3-hk is oxidized easily under physiological conditions, resulting in the production of reactive radical species. to overcome this problem, mosquitoes have developed an efficient mechanism to prevent 3-hk from accumulating by converting this chemically reactive compound to the chemically stable xanthurenic acid. interestingly, 3-hk is a precursor for the production of compou ...200617070835
characteristics of the nuclear (18s, 5.8s, 28s and 5s) and mitochondrial (12s and 16s) rrna genes of apis mellifera (insecta: hymenoptera): structure, organization, and retrotransposable elements.as an accompanying manuscript to the release of the honey bee genome, we report the entire sequence of the nuclear (18s, 5.8s, 28s and 5s) and mitochondrial (12s and 16s) ribosomal rna (rrna)-encoding gene sequences (rdna) and related internally and externally transcribed spacer regions of apis mellifera (insecta: hymenoptera: apocrita). additionally, we predict secondary structures for the mature rrna molecules based on comparative sequence analyses with other arthropod taxa and reference to re ...200617069639
highlights of the xxi annual meeting of the brazilian society of protozoology, the xxxii annual meeting on basic research in chagas' disease & an international symposium on vesicle trafficking in parasitic protozoa--7 to 9 november 2005, caxambu, minas gerais, brazil.this report focuses on the 2005 annual meeting held in caxambu, minas gerais, brazil that was convened and organized by the brazilian society of protozoology http://www.sbpz.org.br/. this is an annual event and details of these meetings can be found on the society's website. within the space available it has been impossible to cover all the important and fascinating contributions and what is presented are our personal views of the meetings scientific highlights and new developments. the contents ...200616916462
[are certain morphological characteristics sufficient to differenciate the varieties of aedes (stegomyia) aegypti linnaeus 1762?importance of the morphological characteristics]. 200614220591
[eradication of aedes aegypti in the island of saint vincent (cape verde archipelago)]. 200613769081
adulticidal activity against stegomyia aegypti (diptera: culicidae) of three piper spp.three piper species, piper longum, p. ribesoides and p. sarmentosum, were selected for investigation of adulticidal potential against stegomyia aegypti, a main vector of dengue and dengue haemorrhagic fever. successive extraction by maceration with 95% ethanol showed percentage yields of ethanolic extracts, which derived from p. longum, p. ribesoides and p. sarmentosum, of 8.89, 3.21 and 5.30% (w/w), respectively. all piper extracts illustrated an impressive adulticidal activity when tested agai ...200616547577
plant-insect interactions. i. biological and phytochemical evaluation of selected plants.extracts from more than 325 different plants have been tested for their effects on larvae of aedes aegypti, and from these initial tests, 16 plants were selected for further study. extracts from plant parts of these 16 plants were screened using tlc and the results are tabulated along with the observed biological activity.20061202312
[poxvirus of aedes aegypti l. mosquitoes]. 20064444602
[study of the aedes aegypti l. densonucleosis virus effect on preimaginal stages of different species of blood-sucking mosquitoes]. 200628461
evaluation of extracts and oils of mosquito (diptera: culicidae) repellent plants from sweden and guinea-bissau.in laboratory tests, ethyl acetate extracts of hyptis suaveolens poit. from guinea-bissau and rhododendon tomentosum (stokes) h. harmaja (formerly ledum palustre l.) and myrica gale l. significantly reduced probing activity of aedes aegypti (l.). in the field in southern sweden, extracts of leaves of r. tomentosum, m. gale, and achillea millefolium l. significantly reduced biting by aedes mosquitoes. volatile compounds from m. gale, r. tomentosum, a. millefolium, and h. suaveolens were collected ...200616506457
[larvicidal activity of tannins isolated of magonia pubescens st. hil. (sapindaceae) against aedes aegypti (diptera, culicidae)].phytochemical study of the larvicidal fractions which were carried out for the first time, isolated of the magonia pubescens, monitored by the study of efficacy against the 3rd larval instar of aedes aegypti, in the search of alternatives for the control of that mosquito and to obtain structures susceptible to chemical improving of the activity for the synthetic via of other derived. the fractions with biological activity were monitored chemically through chromatography of thin layer, using as r ...200615361956
[effect of larvae density on adult size of aedes aegypti reared under laboratory condictions].to evaluate the influence of larval density in development of aedes aegypti mosquitoes we evaluated three different densities. it was observed that conditions of high larval density and shortage of food produced a reduction in the size of adults and increased mortality rate of aedes aegypti in laboratory conditions.200615717099
novel 5beta-hydroxyspirostan-6-ones ecdysteroid antagonists: synthesis and biological testing.eight new 5beta-hydroxy-spirostan-6-ones bearing hydroxy and amino functions in the a ring, i.e., 3beta-oh, 3alpha-oh, 2beta,3beta-oh, 2alpha,3beta-oh, 3beta-nh2, 2alpha-nh2-3beta-oh, 2beta-nh2-3beta-oh, and 2beta-oh-3beta-nh2, were efficiently synthesized, and their antiecdysteroid activities were evaluated on the metamorphosis bioassay of mosquito aedes aegypti. to our knowledge, these new steroids represent the first 5beta-hydroxy-spirostanes which have been tested for antiecdysteroid activit ...200616191434
laboratory and scaled up evaluation of cis-permethrin applied as a new ultra low volume formulation against aedes aegypti (diptera: culicidae).ultra low volume (ulv) aerial spraying is a common methodology for spatial treatments for aedes aegypti (l.) control. previous studies from our laboratory indicated that the cis isomer of permethrin has an excellent efficacy for vector control, in particular for triatoma infestans. it was of interest to determine the efficacy of this pure isomer in a ulv water-based formulation for adult a. aegypti control, since both vectors usually are found together in south america. a method is presented to ...200616174514
the isolated anterior stomach of larval mosquitoes (aedes aegypti): voltage-clamp measurements with a tubular epithelium.the anterior stomach of larval aedes aegypti was isolated and perfused via two pipettes. for transepithelial voltage (v(te)) measurement, the inflow pipette and the bath were connected via agar bridges to calomel electrodes. for voltage-clamping, the lumen of the tissue contained an ag/agcl wire held by the outflow pipette, and the preparation was placed in a bath within a spiral of ag/agcl wire. after equilibrating the tissue in mosquito saline on both sides, a v(te) of -8+/-1 mv was measured ( ...200616310390
[effect of temperature on longevity and infection with the nematode strelkovimermis spiculatus (nemata: mermithidae), a parasite of mosquitoes].strelkovimermis spiculatus is a common parasite of culicid species in argentina. effect of temperature on longevity and infectivity of juvenile preparasites of s. spiculatus was determined at 4, 10, 20 and 27 degrees c. three containers with 100 ml of dechlorinate water and 300 preparasites (12 hour-old), were placed for each day and temperature, during 40 days (total = 480 containers). survived preparasites were counted on 12 containers per day (three for each temperature). when number of survi ...200615162782
[a simulation model for the control of the aedes aegypti, the mosquito vector of dengue and yellow fever, by the crustacean mesocyclops spp].a simulation model is presented to show the predation dynamics of mesocyclops spp. over aedes aegypti.200615106875
comparison of the mosquito-repelling efficacy of methyl neodecanamide (mnda) to that of deet.the objective of this small pilot study was to assess the mosquito-repelling efficacy of methyl neodecanamide (mnda) relative to n,n-diethyl-3-methylbenzamide (deet) against anopheles stephensi, culex quinquefasciatus, and aedes aegypti under controlled laboratory conditions. in this study, subjects inserted both forearms (sham- and repellent-treated) into a test chamber containing 50 female mosquitoes. the number of mosquitoes landing on or probing each forearm during the 5-minute "forced-choic ...200612858227
identification of the aedes aegypti peritrophic matrix protein aeimuci as a heme-binding protein.the gene aedes aegypti intestinal mucin 1 (aeimuc1) encodes a putative peritrophic matrix (pm) protein that is expressed in the midgut of mosquito larvae and adults and is upregulated in response to exposure to heavy metals. the aeimuc1 protein has a predicted secretory signal peptide and three putative chitin-binding domains (cbds) with an intervening mucin-like domain. immunofluorescence and immunoelectron microscopy experiments established that aeimuc1 is a bona fide pm protein, and binding o ...200616878988
analysis of whole body ammonia metabolism in aedes aegypti using [15n]-labeled compounds and mass spectrometry.we have established a protocol to study the kinetics of incorporation of 15n into glutamine (gln), glutamic acid (glu), alanine (ala) and proline (pro) in aedes aegypti females. mosquitoes were fed 3% sucrose solutions containing either 80 mm 15nh4cl or 80 mm glutamine labeled with 15n in either the amide nitrogen or in both amide and amine nitrogens. in some experiments, specific inhibitors of glutamine synthetase or glutamate synthase were added to the feeding solutions. at different times pos ...200616876704
levocetirizine for treatment of immediate and delayed mosquito bite reactions.people frequently experience whealing and delayed papules from mosquito bites. various antihistamines have previously been tried for the treatment of this condition. we performed a double-blind, placebo-controlled, cross-over study with levocetirizine 5 mg and matched placebo in 30 adults who were sensitive to mosquito bites. on the third treatment day the subjects received two aedes aegypti bites on the forearm. the size of the bite lesions and the intensity of pruritus (visual analogue scale) ...200616874418
field evaluation of effectiveness of the bg-sentinel, a new trap for capturing adult aedes aegypti (diptera: culicidae).in recent years, the development of new tools to gather field information about vector ecological parameters has increased. this report evaluated the bg-sentinel trap (bgs-trap), a promising new attempt to improve collection of the dengue vector, aedes aegypti. the efficacy of the bgs-trap was compared with the cdc backpack aspirator, one of the commonest used methods for capturing adult mosquitoes. bgs-traps captured significantly more ae. aegypti males (chi2 = 21.774, df = 1, p < 0.05) and fem ...200616862330
larvicidal effect of pepper plants on aedes aegypti (l.) (diptera: culicidae).ethanolic extracts derived from three species of the piperaceae (pepper) family, piper longum l., p. ribesoides wall., and p. sarmentosum roxb. ex hunt., were evaluated for efficacy against early 4th instar larvae of aedes aegypti mosquitoes using larvicidal bioassays. the highest larvicidal efficacy was established from p. longum, followed by p. sarmentosum and p. ribesoides, with lc50 values of 2.23, 4.06, and 8.13 ppm, respectively. observations of morphological alterations on treated 4th ins ...200616859102
influence of nutritional and physiological status on behavioral responses of aedes aegypti (diptera: culicidae) to deltamethrin and cypermethrin.excito-repellency responses of aedes aegypti (l.) exposed to deltamethrin and cypermethrin were assessed using an excito-repellency test system. contact irritancy and non-contact repellency assays compared non-bloodfed (unfed) parous (post-gravid), nulliparous, early blood-fed, late blood-fed, sugar-fed, and unmated female mosquitoes for behavioral responses based on nutritional and physiological conditions at the time of testing. rates of escape during contact exposure with either compound were ...200616859095
mechanisms of midgut remodeling: juvenile hormone analog methoprene blocks midgut metamorphosis by modulating ecdysone action.in holometabolous insects such as mosquito, aedes aegypti, midgut undergoes remodeling during metamorphosis. insect metamorphosis is regulated by several hormones including juvenile hormone (jh) and 20-hydroxyecdysone (20e). the cellular and molecular events that occur during midgut remodeling were investigated by studying nuclear stained whole mounts and cross-sections of midguts and by monitoring the mrna levels of genes involved in 20e action in methoprene-treated and untreated ae. aegypti. w ...200616829058
composition and larvicidal activity of leaves and stem essential oils of chloroxylon swietenia dc against aedes aegypti and anopheles stephensi.the laboratory bioassay of the essential oil and the isolated compounds from chloroxylon swietenia against aedes aegypti and anopheles stephensi was carried out to evaluate the larvicidal activity. lc50 value estimated for a. aegypti and an. stephensi were 16.5 and 14.9 microg/ml and 20.2 and 19 microg/ml for leaf and stem oils, respectively. the three sesquiterpenes pregeijerene, geijerene and germacrene d were isolated and their larvicidal activity was evaluated. pregeijerene and geijerene wer ...200616815011
larvicidal activity of saponin containing extracts and fractions of fruit mesocarp of balanites aegyptiaca.chloroform, ethyl acetate, butanol and methanol extracts of fruit mesocarp of balanites aegyptiaca and five fractions from the methanol extract were tested against the aedes aegypti mosquito larvae. all extracts showed larvicidal effects however the highest larval mortality was found in methanol extract. one fraction obtained from the silica gel column chromatography of the latter was found most effective for larval mortality. this fraction also interfered with adult emergence. a concentration o ...200616814957
larvicidal diterpenes from pterodon polygalaeflorus.ethanolic extract from seeds of pterodon polygalaeflorus (benth) has been shown to possess significant larvicidal activity against the mosquito aedes aegypti. bioassay-guided fractionation of the extract led to the isolation and characterization of the know diterpenoid furans 6alpha-hydroxyvouacapan-7beta,17beta-lactone (1), 6alpha,7beta-dihydroxyvouacapan- 17beta-oic acid (2) and methyl 6alpha,7beta-dihydroxyvouacapan-17beta-oate (3). the structures were established from infrared (ir), ultravio ...200616796519
caffeine effect on mortality and oviposition in successive generations of aedes aegypti.previous experiments showed that caffeine blocks the development of aedes aegypti (diptera, culicidae) in the larval stage, consequently inhibiting the production of adults. this study aimed at obtaining data suggestive of caffeine resistance by these mosquitoes.200617173171
xanthones from garcinia mangostana (guttiferae).studies on the stem of garcinia mangostana have led to the isolation of one new xanthone mangosharin (1) (2,6-dihydroxy-8-methoxy-5-(3-methylbut-2-enyl)-xanthone) and six other prenylated xanthones, alpha-mangostin (2), beta-mangostin (3), garcinone d (4), 1,6-dihydroxy-3,7-dimethoxy-2-(3-methylbut-2-enyl)-xanthone (5), mangostanol (6) and 5,9-dihydroxy-8- methoxy-2,2-dimethyl-7-(3-methylbut-2-enyl)-2h,6h-pyrano-[3,2-b]-xanthene-6-one (7). the structures of these compounds were determined by spe ...200617127660
effect of stalk and leaf extracts from euphorbiaceae species on aedes aegypti (diptera, culicidae) larvae.the objective of this study was to evaluate the larvicidal activity of essential oil aqueous solutions (hydrolates) obtained by steam distillation of stalks and leaves of croton argyrophylloides, croton nepetaefolius, croton sonderianus and croton zehntneri against aedes aegypti larvae. twenty-five larvae of third instar were placed in plastic beckers, containing the hydrolates (50 ml), in a four repetitions scheme. water was used as control and the number of dead larvae was counted after 24 hou ...200617119677
p-type na+/k+-atpase and v-type h+-atpase expression patterns in the osmoregulatory organs of larval and adult mosquito aedes aegypti.this study describes the expression patterns of p-type na(+)/k(+)-atpase and v-type h(+)-atpase in the larval and adult forms of the mosquito aedes aegypti and provides insight into their relative importance in ion transport function of key osmoregulatory organs. rt-pcr assays indicate that, at the level of the gene, both atpases are expressed in all of the osmoregulatory tissues of larvae (midgut, malpighian tubules, rectum and anal papillae) and adults (stomach, malpighian tubules, anterior hi ...200617114398
ecdysteroid titers and developmental expression of ecdysteroid-regulated genes during metamorphosis of the yellow fever mosquito, aedes aegypti (diptera: culicidae).ecdysteroid titers and expression profiles of ecdysone-regulated genes were determined during the last instar larval and during the pupal stages of aedes aegypti (diptera: culicidae). three peaks of ecdysteroids occurring at approximately 24, 30-33 and 45-48h after ecdysis to the fourth instar larval stage were detected. in the pupa, a large peak of ecdysteroids occurred between 6 and 12h after ecdysis to the pupal stage. a small rise in ecdysteroids was also detected at the end of the pupal sta ...200616580015
ultra low volume aerosol application of deltacide (deltamethrin 0.5% w/v, s-bioallethrin 0.71% w/v & piperonyl butoxide 8.9% w/v) against mosquitoes.insecticide in the form of space spray as an ultra low volume (ulv) aerosol are used during epidemics of vector borne diseases. deltacide, a formulation comprising of three chemicals viz., deltamethrin 0.5 per cent w/v, s-bio-allethrin 0.71 per cent w/v and piperonyl butoxide 8.9 per cent w/v is suitable for ulv application. as this combination is found to be effective in preventing resistance development tackling the population, which had already developed resistance and cause immediate mortali ...200616567869
reevaluation of the role of early trypsin activity in the transcriptional activation of the late trypsin gene in the mosquito aedes aegypti.in the female mosquito aedes aegypti, trypsin expression is largely biphasic. early trypsin synthesis, which is regulated at the translational level relative to feeding, peaks in the first few hours post-blood meal. late trypsin expression is regulated at the transcriptional level, and peaks 18-24h post-blood meal. it was proposed that early trypsin activity released unknown factors during digestion of a meal that caused activation of transcription of the late trypsin gene. this connection betwe ...200616551547
regulatory region of the vitellogenin receptor gene sufficient for high-level, germ line cell-specific ovarian expression in transgenic aedes aegypti mosquitoes.vitellogenin receptor (vgr) is responsible for the receptor-mediated endocytosis of vitellogenin (vg) in the egg formation of an oviparous animal, including insects. little is known about regulation of vgr gene expression. we analyzed the upstream region of the vgr gene from aedes aegypti (aavgr) to identify regulatory elements responsible for its expression in germ cell-specific ovarian expression. experiments with genetic transformation using the transgene containing the 1.5-kb upstream portio ...200616551541
regulation of the ribonucleotide reductase small subunit (r2) in the yellow fever mosquito, aedes aegypti.ribonucleotide reductase (rnr) catalyzes the formation of deoxyribonucleotides, a rate limiting step in dna synthesis. class i rnr is a tetramer that consists of two subunits, r1 and r2; enzymatic activity requires association of r1 with r2. the r2 subunit is of special interest because it dictates the interaction with r1 that is required for enzymatic activity expression, and it is expressed only during the s phase of the cell cycle. we previously sequenced an r2 cdna clone from the yellow feve ...200616530987
tetraethylammonium and nicotine transport by the malpighian tubules of insects.we examined transepithelial transport of the prototypical type i organic cation (oc) tetraethylammonium (tea) and the plant alkaloid nicotine by the isolated malpighian tubules (mts) of nine insect species from six orders. isolated tubules were exposed to radiolabelled forms of either tea or nicotine in the bathing (basal) fluid. luminal (apical) secreted fluid was collected and tea or nicotine concentration was determined. active net transport of nicotine from bath to lumen was observed by the ...200616527303
investigation of cuticular hydrocarbons for determining the age and survivorship of australasian mosquitoes.the cuticular hydrocarbon (ch) technique of age grading mosquitoes was evaluated for use on laboratory-reared aedes aegypti (l.), anopheles farauti (laveran), and ochlerotatus vigilax (skuse). gas chromatography/mass spectroscopy was used to determine the relative abundance of five alkanes in hexane extracts from the legs of individual mosquitoes. age-related changes to relative hydrocarbon abundances were observed from an. farauti and ae. aegypti, which are vectors of malaria and dengue, respec ...200616525108
a new recombinant mosquito salivary allergen, raed a 2: allergenicity, clinical relevance, and cross-reactivity.mosquito salivary proteins cause allergic reactions in humans. the allergenicity, clinical relevance, and species cross-reactivity of a new 37-kda recombinant mosquito (aedes aegypti) salivary allergen, raed a 2, were evaluated.200616512811
crystal structures of aedes aegypti alanine glyoxylate aminotransferase.mosquitoes are unique in having evolved two alanine glyoxylate aminotransferases (agts). one is 3-hydroxykynurenine transaminase (hkt), which is primarily responsible for catalyzing the transamination of 3-hydroxykynurenine (3-hk) to xanthurenic acid (xa). interestingly, xa is used by malaria parasites as a chemical trigger for their development within the mosquito. this 3-hk to xa conversion is considered the major mechanism mosquitoes use to detoxify the chemically reactive and potentially tox ...200616990263
sterculia guttata seeds extractives--an effective mosquito larvicide.the larvicidal activity of ethanol, chloroform and hexane soxhlet extracts obtained from s. guttata seeds was investigated against the ivth instar larvae of dengue fever vector, aedes aegypti and filarial vector, culex quinquefasciatus. all extracts including fractions of ethanol extract exhibited 100% larval kill within 24 hr exposure period at 500 ppm concentration. fraction a1 of ethanol was found to be most promising; its lc50 was 21.552 and 35.520 ppm against c. quinquefasciatus and a. aegy ...200616924837
abridged pupa identification key to the common container-breeding mosquitoes in urban southeast asia.pupal surveys have been advocated as an alternative or surrogate surveillance method for estimating densities of adult aedes aegypti and aedes albopictus. usually, this survey strategy has required that collected pupae eclose to adults before attempting species identification. using the pupal survey method in yogyakarta, indonesia, this rearing step was obviated with the pupal morphological key described herein for identifying preserved or live pupae. examination of pupae for the identification ...200617067066
olfactometer and large cage evaluation of a solid phase technology for the controlled production of co2.new technology by ica for chemical generation of carbon dioxide (co2) was evaluated in olfactometer and large outdoor cage tests against laboratory-reared aedes aegypti for potential use in mosquito surveillance programs. the proprietary co2 generation system consists of a poly-tyvek sachet containing 2 solid ingredients. activated sachets immediately react to generate and release predictable levels of co2 over time. in noncompetitive olfactometer studies, a freshly activated sachet attracted an ...200617067034
cloning and daily expression of the timeless gene in aedes aegypti (diptera:culicidae).molecular approaches for studying biological rhythms in insects have been well investigated in the model drosophila melanogaster, in which a number of genes have been characterized in terms of sequence, expression, protein interactions and involvement in the control of locomotor activity and eclosion rhythms. however, only scattered information is available for insect vectors of diseases. in this paper, we report the cloning and expression analysis of the clock gene timeless in the mosquito aede ...200617046601
lethal and behavioural effects of three synthetic repellents (deet, ir3535 and kbr 3023) on aedes aegypti mosquitoes in laboratory assays.the knock-down, mortality and 'irritancy' effects of three synthetic repellents (deet, ir3535 and kbr 3023) on aedes aegypti (l) (diptera: culicidae) were evaluated in the laboratory in the absence of animal bait. filter paper tests were carried out to assess the knock-down effect (kdt(50) and kdt(95)) and mortality (lc(50) and lc(95)) induced by each repellent. 'irritancy' tests were carried out to compare the flight response (time to first take-off, or ft) to increasing concentrations of repel ...200617044879
detection of insecticides resistance status in culex quinquefasciatus and aedes aegypti to four major groups of insecticides.the resistance to various insecticides from 4 major groups (organochlorine, organophosphate, carbamate and pyrethroid) was investigated in a field strain of culex quinquefasciatus from baan suan community, nonthaburi province, thailand by using a standard world health organization susceptibility test. the baan suan strain was completely resistant to ddt and highly resistant to deltamethrin, permethrin, fenitrothion and propoxur but this strain was still found to be highly susceptible to malathio ...200617041557
structural organization of the mitochondrial dna control region in aedes aegypti.the complete a+t - rich region of aedes aegypti mitochondrial dna has been cloned and sequenced. in argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. nucleotide sequence comparison of the shortest and longest a+t - rich amplified fragments detected revealed the presence of 2 types of tandemly repeated blocks. the size variation observed in natural populations is mainly due to the presence of a variable number of a 181 bp tandem repeat un ...200617036068
role of egg buster in hatching of aedes aegypti: scanning electron microscopy study.egg hatching has been studied in aedes aegypti (l.) through scanning electron microscopy. the first sign of egg hatching is a small protrusion on the eggshell in the anterior pole. the larval movement provokes a crack in the eggshell with the egg buster located in the dorsal head. the egg buster provokes a small transverse fissure in the eggshell that gradually increases in the chorion. then, the rupture is completed around the eggshell. the separation of the anterior pole occurs, showing the do ...200616506449
secreted ferritin: mosquito defense against iron overload?the yellow fever mosquito, aedes aegypti, must blood feed in order to complete her life cycle. the blood meal provides a high level of iron that is required for egg development. we are interested in developing control strategies that interfere with this process. we show that a. aegypti larval cells synthesize and secrete ferritin in response to iron exposure. cytoplasmic ferritin is maximal at low levels of iron, consists of both the light chain (lch) and heavy chain (hch) subunits and reflects ...200616503479
involvement of cytochrome p450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics.the response of mosquito larvae to plant toxins found in their breeding sites was investigated by using aedes aegypti larvae and toxic arborescent leaf litter as experimental models. the relation between larval tolerance to toxic leaf litter and cytochrome p450 monooxygenases (p450s) was examined at the toxicological, biochemical and molecular levels. larvae pre-exposed to toxic leaf litter show a higher tolerance to those xenobiotics together with a strong increase in p450 activity levels. this ...200616651188
comparative efficacy and persistency of permethrin in olyset net and conventionally treated net against aedes aegypti and anopheles stephensi.the efficacy of the olyset net was compared to a net treated conventionally with permethrin 10% emulsifiable concentrate at the world health organization recommended dose. the nets were assessed under laboratory conditions against the mosquito vectors aedes aegypti and anopheles stephensi. knockdown efficacy for the olyset net was assessed after the first 10 washings and then after the 15th and 20th washings. data were not collected for the conventionally treated netting after 5 washings because ...200616646331
in vivo and in vitro activity of venom from the endoparasitic wasp pimpla turionellae (l.) (hymenoptera: ichneumonidae).the biological activity of venom from pimpla turionellae l. (hymenoptera: ichneumonidae) was examined in vivo toward larvae and pupae of galleriae mellonella l. (lepidoptera: pyralidae), and in vitro toward bacterial and fungal cultures, as well as cultured insect cells. pupae of g. mellonella were far more susceptible to the venom than larvae. at low doses of venom [0.1 venom reservoir equivalents (vre)], pupal abdominal mobility was inhibited within 30 min, and by 24 h, all pupae injected with ...200616416450
regulatory mechanisms of chitin biosynthesis and roles of chitin in peritrophic matrix formation in the midgut of adult aedes aegypti.in mosquitoes, the peritrophic matrix is formed in response to blood feeding and can be a physical barrier when pathogens ingested with blood meal attempt to reach and transverse the midgut epithelium. the main components of the peritrophic matrix are chitin-biding-domain containing proteins, glycosylated proteins, and chitin fibrils. chitin is synthesized from fructose-6-phosphate by a series of five enzymatic reactions. we previously found that blood feeding induces transcriptional up-regulati ...200616360944
effects of larval nutrition on the endocrinology of mosquito egg development.reproduction by female mosquitoes is dependent on energy resources but modulated by hormones. our study focused on blood-meal-dependent, anautogenous aedes aegypti and autogenous ochlerotatus atropalpus that rely on larval-derived nutrient stores to develop eggs. to determine how larval nutrition affects the endocrinology of egg development in these females, we manipulated the quantity of larval food and measured in vitro production of juvenile hormone (jh) by corpora allata (ca) and ecdysteroid ...200616449559
qsar study of mosquito repellents using codessa pro.protection times provided by 31 synthetic repellents against aedes aegypti mosquitoes were correlated with the chemical structures of these repellents using codessa pro software. two statistically significant quantitative models with r2 values of ca. 0.80 are presented and discussed.200616488605
laboratory bioefficacy of creek 1.0g (temephos) against aedes (stegomyia) aegypti (linnaeus) larvae.the bioefficacy of a commercial formulation of temephos, creek against aedes aegypti larvae was studied in the laboratory. earthen jars were filled with 10 l tap water each. one g of temephos (creek) sand granule formulation was added into each earthen jar as recommended by the manufacturer. the final test concentration of creek was 1 mg a.i./l. one earthen jar was filled with 10 l tap water and served as a test control (untreated). thirty late 3(rd) or early 4(th) instar of lab-bred ae. aegypti ...200617322825
efficacy of botanical extracts from callitris glaucophylla, against aedes aegypti and culex annulirostris mosquitoes.using standard who methodology, this study investigated the susceptibility of 4(th) instar aedes aegypti (l) and culex annulirostris (skuse) larvae to three extracts from callitris glaucophylla (j. thompson & l. johnson) (1: steam distillation extract, 2: liquefied refrigerant gas extract, and 3: methanol reflux extract), lambda-cyhalothrin (a synthetic pyrethroid insecticide) and fenitrothion (an organophosphorous insecticide). cx. annulirostris was significantly more susceptible than ae. aegyp ...200617322820
larvicidal effect of andiroba oil, carapa guianensis (meliaceae), against aedes aegypti.the aim of this work was to evaluate the larvicidal effect of andiroba oil, carapa guyanensis, against 2 strains of aedes aegypti. after 8 h after exposure to oil, the lethal concentration (lc)90 and lc95 values for the gcz (temephos-resistant) strain larvae were 80 and 86 ppm (1st instars), 98 and 106 (2nd instars), 166 and 182 (3rd instars), and 192 and 202 ppm (4th instars), respectively. thelc90 and lc95 values for the rockefeller strain larvae were 164 and 182 ppm (1st instars), 212 and 224 ...200617304939
optimizing ovitrap use for aedes aegypti in cairns, queensland, australia: effects of some abiotic factors on field efficacy.insecticide-treated lethal ovitraps are used for control of the dengue vector aedes aegypti in north queensland, australia. in an effort to optimize their use, the influence of deployment height, premise shading, and protection from wind on trap efficacy was assessed in field experiments. sticky ovitraps were used as a proxy for lethal ovitraps because they provide a direct measure of adult visitation rates. sticky ovitraps deployed at ground level for 1 wk captured significantly more female ae. ...200617304930
isolation and characterization of the rangap gene in the mosquito aedes aegypti.a duplicated 3'-truncated version of rangap was previously identified as segregation distorter (sd), the meiotic drive gene in drosophila melanogaster. here we report the cloning and characterization of the complete gene sequence for the rangap homolog from the mosquito aedes aegypti. the 1995 bp cdna sequence consists of a 113 bp 5' utr and 130 bp 3' utr, and encodes a 583 amino acid protein with high sequence identity with rangap homologues of several species. a 20,125 bp genomic dna sequence ...200617286051
parasitoid-induced behavioral alterations of aedes aegypti mosquito larvae infected with mermithid nematodes (nematoda: mermithidae).a wide range of parasites are known to cause behavioral changes in their hosts and parasitized insects are especially amenable to the study of such changes. the majority of studies addressing parasite-induced behavioral alterations have focused on parasites with complex life cycles and the adaptive nature of such changes. behavioral changes caused by parasitoids, single-host parasites that kill their host upon emergence, have been studied less and the adaptive nature of these changes is likely t ...200617249352
larvicidal activity of bis(2-ethylhexyl) benzene-1,2-dicarboxylate from sterculia guttata seeds against two mosquito species.the larvicidal activities of various fractions of the hexane extract of the seeds of sterculia guttata against larvae of aedes aegypti and culex quinquefasciatus were determined. bis(2-ethylhexyl) benzene-1,2-dicarboxylate (1) was identified as one of the active principles, displaying chronic toxicity against both types of larvae, with ld50 values of 79 and 64 ppm, respectively.200617193215
scanning electron microscopy of the four larval instars of the dengue fever vector aedes aegypti (diptera: culicidae).aedes aegypti is the main insect vector of dengue fever and dengue hemorrhagic fever/dengue shock syndrome and represents the only vulnerable element in the control of this disease. therefore, the identification and quantification of this mosquito is an important task; however, the majority of taxonomic keys are based on the 4th larval instar. for that reason, this study describes the four larval instars ofa. aegypti using scanning electron microscopy. morphological changes during larval develop ...200618491625
scanning electron microscopy of damage caused by mesocyclops thermocyclopoides (copepoda: cyclopoidea) on larvae of the dengue fever vector aedes aegypti (diptera: culicidae).dengue fever is a mosquito-borne viral disease, whose main biological vector is aedes aegypti. this mosquito colonizes tropical areas where the disease is endemic. the most obvious action against dengue is attacking its vector. biological control appears to be an alternative approach, using natural enemies of the mosquitoes, such as predatory copepods. thus, the morphological study of the damage caused by copepods is important to understand its predatory capacity. twenty-five a. aegypti larvae w ...200618491624
[the effect of the period of egg quiescence on the life cycle of aedes aegypti (linnaeus, 1762) (diptera, culicidae) under laboratory conditions].the influence of the period of egg quiescence on the life cycle of aedes aegypti (linnaeus, 1762) (diptera, culicidae) was studied under laboratory conditions in order to improve the management of vector control. the eggs are known to be the most resistant stages during development, allowing a long survival of the mosquitoes under unfavorable climatic conditions. the experiments were performed in a biological chamber kept at 28 +/- 1 degrees c temperature, with 80 +/- 5% relative humidity and 12 ...200610495662
[utilization of the waste of sisal industry in the control of mosquito larvae].the aim of this research was to utilize the waste residues of sisal fiber separation from agave sisalana leaves to develop a larvicide for the combat of mosquito transmitting tropical diseases. larvae of aedes aegypti and culex quinquefasciatus were exposed to different concentrations of the agave extract for 24 hours to determine lethal concentrations. the lc50 for a. aegypti was 322 ppm and the lc50 for c. quinquefasciatus was 183 ppm. to detect the active substances, saponins were investigate ...20069927821
[use of surviving infectious larvae of dipetalonema dessetae in study and research on filaricidal substances].dipetalonema dessetae in proechimys oris, the natural final host is a rodent filariasis model used as an in vivo antifilarial screening test. the laboratory vector is aedes aegypti. infective larvae l3 isolated from the intermediate host develop and remain healthy for up to 30 days in a biphasic culture medium composed of a cell feeder layer (l 929) and rpmi 1640 supplemented with foetal calf serum. this culture technique has enabled us to screen antifilarial compounds on a new in vitro test. th ...20063795974
[dipetalonema dessetae--aedes aegypti: study of the infected adipose tissue (author's transl)].histological and ultrastructural study of the adipose tissue of aedes aegypti has shown that infection with the filaria dipetalonema dessetae locally induces a highly active synthesis of proteins (formation of ribosomes, endoplasmic reticulum and golgi complexes, multiplication of mitochondria). this phenomenon appears similar to the effect of beta-ecdysone after the up-take of blood. mosquitoes let to starve during the development of the filaria show a quick disappearance of storage substances ...2006573582
[effect of dexonucleosis virus on the imago of aedes aegypti]. 20061004291
argentophilic structures of spermatogenesis in the yellow fever mosquito.application of the silver-staining technique to air-dried chromosome preparations of the yellow fever mosquito, aedes aegypti, revealed the following: 1) intensely stained pericentromeric regions in all chromosomes including the y chromosome in spermatogonia, and during the primary and secondary spermatocytes; 2) the presence of prepachytene that were not reported earlier; 3) a nucleolus organizing region that persisted up to the late pachytene stage; and 4) rod or ring-like centrioles in pachyt ...20064031466
spatial and temporal structure of typhoid outbreaks in washington, d.c., 1906-1909: evaluating local clustering with the gi* statistic.to better understand the distribution of typhoid outbreaks in washington, d.c., the u.s. public health service (phs) conducted four investigations of typhoid fever. these studies included maps of cases reported between 1 may - 31 october 1906 - 1909. these data were entered into a gis database and analyzed using ripley's k-function followed by the gi* statistic in yearly intervals to evaluate spatial clustering, the scale of clustering, and the temporal stability of these clusters.200616566830
minor proteins and enzymes of the drosophila eggshell matrix.the drosophila eggshell provides an in vivo model system for extracellular matrix assembly, in which programmed gene expression, cell migrations, extracellular protein trafficking, proteolytic processing, and cross-linking are all required to generate a multi-layered and regionally complex architecture. while abundant structural components of the eggshell are known and are being characterized, less is known about non-abundant structural, regulatory, and enzymatic components that are likely to pl ...200616515779
the evolution of the histone methyltransferase gene su(var)3-9 in metazoans includes a fusion with and a re-fission from a functionally unrelated gene.in eukaryotes, histone h3 lysine 9 (h3k9) methylation is a common mechanism involved in gene silencing and the establishment of heterochromatin. the loci of the major heterochromatic h3k9 methyltransferase su(var)3-9 and the functionally unrelated gamma subunit of the translation initiation factor eif2 are fused in drosophila melanogaster. here we examined the phylogenetic distribution of this unusual gene fusion and the molecular evolution of the h3k9 hmtase su(var)3-9.200616512904
new findings on the developmental process of ascogregarina taiwanensis and ascogregarina culicis in aedes albopictus and aedes aegypti.infection in different stages of larvae of aedes aegypti and ae. albopictus with ascogregarina taiwanensis and a. culicis, respectively, revealed that the oocysts of ascogregarina spp. are able to infect any instar and can complete their life cycle within 9.5 +/- 1 days. when early instars ingested oocysts, parasite development was synchronized to larval-pupal ecdysis and oocyst dissemination occurred at the time of adult emergence, oviposition, or both. the parasites also developed normally whe ...200616646318
assessment of toxicity of some penta- and hexacoordinated organotin(iv) and tetracoordinated tin(ii) complexes of heterocyclic beta-diketones.a number of penta- and hexacoordinated organotin(iv) complexes and tetracoordinated tin(ii) complexes of compositions me2sncl[rcoc:con(c6h5)n:cch3] (where r = - ch(3), -p-clc(6)h(4), and -c(6)h(5)), me2sn[rcoc:con(c6h5)n:cch3]2 (where r = -ch(3), and -c(6)h(5)), and sn(ii) [rcoc:con(c6h5)n:cch3]2 (where r = -p-clc(6)h(4) and -c(6)h(5)) were screened for their toxicity against musca domestica (house fly). in general, organotin(iv) complexes contribute more to the activity than tin(ii) complexes.200617497010
the mushroom body defect gene product is an essential component of the meiosis ii spindle apparatus in drosophila oocytes.in addition to their well-known effects on the development of the mushroom body, mud mutants are also female sterile. here we show that, although the early steps of ovary development are grossly normal, a defect becomes apparent in meiosis ii when the two component spindles fail to cohere and align properly. the products of meiosis are consequently mispositioned within the egg and, with or without fertilization, soon undergo asynchronous and spatially disorganized replication. in wild-type eggs, ...200616510791
construction of a single nucleotide polymorphism linkage map for the silkworm, bombyx mori, based on bacterial artificial chromosome end sequences.we have developed a linkage map for the silkworm bombyx mori based on single nucleotide polymorphisms (snps) between strains p50t and c108t initially found on regions corresponding to the end sequences of bacterial artificial chromosome (bac) clones. using 190 segregants from a backcross of a p50t female x an f1 (p50t x c108t) male, we analyzed segregation patterns of 534 snps between p50t and c108t, detected among 3840 pcr amplicons, each associated with a p50t bac end sequence. this enabled us ...200616547112
genetically engineered underdominance for manipulation of pest populations: a deterministic model.we theoretically investigate the potential for introgressing a desired engineered gene into a pest population by linking the desired gene to dna constructs that exhibit underdominance properties. our deterministic model includes two independently segregating engineered constructs that both carry a lethal gene, but suppress each other. only genotypes containing both or neither construct are viable. both constructs also carry the desired gene with an independent regulatory mechanism. we examine th ...200616415364
role of juvenile hormone and allatotropin on nutrient allocation, ovarian development and survivorship in mosquitoes.teneral reserves are utilized to initiate previtellogenic ovarian development in mosquitoes. females having emerged with low teneral reserves have reduced juvenile hormone (jh) synthesis and previtellogenic development. we investigated what role jh, allatotropin (at) and other head-factors play in the regulation of previtellogenic ovarian development and adult survivorship. factors from the head are essential for corpora allata (ca) activation and reproductive maturation. we have shown that deca ...200617070832
evolution of glyoxylate cycle enzymes in metazoa: evidence of multiple horizontal transfer events and pseudogene formation.the glyoxylate cycle is thought to be present in bacteria, protists, plants, fungi, and nematodes, but not in other metazoa. however, activity of the glyoxylate cycle enzymes, malate synthase (ms) and isocitrate lyase (icl), in animal tissues has been reported. in order to clarify the status of the ms and icl genes in animals and get an insight into their evolution, we undertook a comparative-genomic study.200617059607
the competence factor beta ftz-f1 potentiates ecdysone receptor activity via recruiting a p160/src coactivator.hormones provide generalized signals that are interpreted in a specific spatial and temporal manner by a developing or reproducing multicellular organism. the ability to respond to hormones is determined by the competence of a cell or a tissue. the betaftz-f1 orphan nuclear receptor acts as a competence factor for the steroid hormone 20-hydroxyecdysone (20e) in drosophila melanogaster metamorphosis and mosquito reproduction. the molecular nature of the betaftz-f1 action remains unclear. we repor ...200617015464
direct and indirect effects of animal detritus on growth, survival, and mass of invasive container mosquito aedes albopictus (diptera: culicidae).compared with plant detritus, animal detritus yields higher growth rates, survival, adult mass, and population growth of container-dwelling mosquitoes. it is unclear whether the benefit from animal detritus to larvae results from greater microorganism growth, direct ingestion of animal detritus by larvae, or some other mechanism. we tested alternative mechanisms by which animal detritus may benefit the invasive container-dwelling mosquito aedes albopictus (skuse) (diptera: culicidae). in the lab ...200717695011
interspecific and intraspecific differences in foraging preferences of container-dwelling mosquitoes.feeding preferences of larval container-dwelling mosquitoes are not well understood. primary production is often absent in container systems and external inputs of animal and plant detritus supply the energy base of container food webs by supporting microorganism prey for mosquitoes. we quantified the feeding preferences of aedes albopictus (skuse), a non-native invasive mosquito, and ochlerotatus triseriatus (say), a native mosquito, when given a choice of animal and plant detritus. we tested f ...200717427689
temperature-dependent development and survival rates of culex quinquefasciatus and aedes aegypti (diptera: culicidae).development, growth, and survival of culex quinquefasciatus say and aedes aegypti (l.) were determined at six constant temperatures (15, 20, 25, 27, 30, 34 degrees c). the sharpe & demichele four-parameter model with high-temperature inhibition described the temperature-dependent median developmental rates of both mosquito species. in both species, body size generally decreased as temperature increased. head capsule widths in all instars in both species were significantly greater at 15 than at 3 ...20072231624
an insight into the sialome of the blood-sucking bug triatoma infestans, a vector of chagas' disease.triatoma infestans is a hemiptera, vector of chagas' disease that feeds exclusively on vertebrate blood in all life stages. hematophagous insects' salivary glands (sg) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. to obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cdna library from its sg was randomly sequenced. also, salivary proteins were submitt ...200718207082
surveillance of arthropod vector-borne infectious diseases using remote sensing techniques: a review.epidemiologists are adopting new remote sensing techniques to study a variety of vector-borne diseases. associations between satellite-derived environmental variables such as temperature, humidity, and land cover type and vector density are used to identify and characterize vector habitats. the convergence of factors such as the availability of multi-temporal satellite data and georeferenced epidemiological data, collaboration between remote sensing scientists and biologists, and the availabilit ...200717967056
genetic variation in multilocus microsatellite genotypes in two species of woodrats (neotoma macrotis and n. fuscipes) from california.five microsatellite loci were used to develop multilocus genotypes for neotoma macrotis (n = 128) and n. fuscipes (n = 29). several statistical analyses were used to estimate genetic structure, levels of genetic variability, and degree of relatedness within groups of these 2 species. samples of n. macrotis represented 2 groups and 4 population clusters throughout southern california. samples of n. fuscipes represented 2 regions in northern and southern california. genetic structure was detected ...200719920871
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