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susceptibility comparisons of normal preoperative conjunctival bacteria to fluoroquinolones.to compare the susceptibility and efficacy of 3 fluoroquinolones, (levofloxacin, gatifloxacin, and moxifloxacin) in treating conjunctival bacteria in patients having ocular surgery.200919251140
infective endocarditis due to propionibacterium species.propionibacterium species rarely cause infective endocarditis. when identified in blood cultures, they may be inappropriately disregarded as skin flora contaminants. the purpose of this study was to characterize the clinical presentation and management of endocarditis due to propionibacterium species. all cases of endocarditis due to propionibacterium species that were treated at the mayo clinic, rochester, usa were retrospectively reviewed, and the english language medical literature was search ...200919260876
microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication.we recently described a sonication technique for the diagnosis of prosthetic knee and hip infections. we compared periprosthetic tissue culture to implant sonication followed by sonicate fluid culture for the diagnosis of prosthetic shoulder infection. one hundred thirty-six patients undergoing arthroplasty revision or resection were studied; 33 had definite prosthetic shoulder infections and 2 had probable prosthetic shoulder infections. sonicate fluid culture was more sensitive than periprosth ...200919261785
pilot study of association of bacteria on breast implants with capsular contracture.capsular contracture is the most common and frustrating complication in women who have undergone breast implantation. its cause and, accordingly, treatment and prevention remain to be elucidated fully. the aim of this prospective observational pilot study was to test the hypothesis that the presence of bacteria on breast implants is associated with capsular contracture. we prospectively studied consecutive patients who underwent breast implant removal for reasons other than overt infection at th ...200919261794
chemical composition and biological activities of jeju thymus quinquecostatus essential oils against propionibacterium species inducing acne. 200919282635
involvement of propionibacterium acnes in the augmentation of lipogenesis in hamster sebaceous glands in vivo and in vitro.propionibacterium acnes is considered to be involved in the aggravation of acne vulgaris, but it remains unclear whether p. acnes directly influences lipogenesis in sebaceous glands. in this study, we showed that a culture medium of p. acnes (acnes-cm) and formalin-killed p. acnes (f-acnes) prepared from p. acnes strains, jcm6473 and jcm6425, intracellularly augmented lipid droplet formation and triacylglycerol (tg) synthesis in undifferentiated and insulin-differentiated hamster sebocytes. acne ...200919282842
isolation of propionibacterium acnes from a case of placentitis and abortion in a cow.on the basis of the scarcity of reports in the veterinary literature, it appears that propionibacterium spp. are rarely associated with disease or isolated from cattle tissues. recently, propionibacterium spp. has been associated with multifocal abscessation in cattle. this report describes a case of necrosuppurative placentitis and abortion in an adult holstein cow. numerous colonies of small, pleomorphic, gram-positive, rod-shaped bacteria were observed within the fibrin lattice associated wit ...200919286514
4-1bb functions as a survival factor in dendritic cells.4-1bb (cd137) is expressed on dendritic cells (dcs) and its biological function has remained largely unresolved. by comparing 4-1bb-intact (4-1bb(+/+)) and 4-1bb-deficient (4-1bb(-/-)) dcs, we found that 4-1bb was strongly induced on dcs during the maturation and that dc maturation was normal in the absence of 4-1bb. however, dc survival rate was low in the absence of 4-1bb, which was due to the decreased bcl-2 and bcl-x(l) in 4-1bb(-/-) dcs compared with 4-1bb(+/+) dcs after dc maturation. cons ...200919299708
acute-onset nosocomial endophthalmitis after cataract surgery: incidence, clinical features, causative organisms, and visual outcomes.to estimate the incidence of acute-onset endophthalmitis after cataract surgery and to report its clinical features, microbiology, and final visual outcomes.200919304084
sonication of removed breast implants for improved detection of subclinical infection.capsular fibrosis is a severe complication after breast implantation with an uncertain etiology. microbial colonization of the prosthesis is hypothesized as a possible reason for the low-grade infection and subsequent capsular fibrosis. current diagnostic tests consist of intraoperative swabs and tissue biopsies. sonication of removed implants may improve the diagnosis of implant infection by detachment of biofilms from the implant surface.200919322605
the novel anti-propionibacterium acnes compound, sargafuran, found in the marine brown alga sargassum macrocarpum.we screened extracts of 342 species of marine algae collected from japanese coastlines for antibacterial activity against propionibacterium acnes, and found a novel antibacterial compound, which we named sargafuran, from the meoh extract of the marine brown alga, sargassum macrocarpum. sargafuran has low cytotoxicity, and the mic against p. acnes was 15 microg ml(-1), showing a broad antibacterial activity against gram-positive bacteria. a time-kill study showed that sargafuran was bactericidal ...200919329987
lid and conjunctival micro biota during contact lens wear in children.to determine the types of microorganisms and their frequency of isolation on the external ocular surface in children wearing soft contact lenses on a daily wear schedule.200919348019
effects of propionibacterium acnes on various mrna expression levels in normal human epidermal keratinocytes in vitro.propionibacterium acnes is one of the most significant pathogenic factors of acne vulgaris. this bacteria relates to acne by various pathways. it has also been reported that p. acnes influences pro-inflammatory cytokine production in keratinocytes in vitro. however, the influence on the differentiation of keratinocytes by p. acnes has not been studied extensively. we analyzed the expression of keratinocyte differentiation-specific markers, keratins, and pro-inflammatory cytokines in normal human ...200919348660
lowering lesional surface ph in acne: a new treatment modality for herpifix.the acid skin surface ph has antimicrobial activities. increased growth of propionibacterium acnes contributes to the pathogenesis of acne. therefore, the ph of inflammatory acne lesions was determined prior to and after lesional acidification employing herpifix (courage + khazaka, cologne, germany), a microphoretic system. the ph was correlated with the number of acne lesions. a total of 30 volunteers with acne vulgaris participated in this crossover study applying either herpifix or a dummy to ...200918618363
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
purulent pericarditis: report of 2 cases and review of the literature.purulent pericarditis, a localized infection within the pericardial space, has become a rare entity in the modern antibiotic era. although historically a disease of children and young adults, this is no longer the case: the median age at the time of diagnosis has increased by nearly 30 years over the past 6 decades. despite advances in diagnostic and treatment modalities, purulent pericarditis remains a life-threatening illness. unfortunately, the diagnosis is made postmortem in more than half t ...200919352300
propionibacterium acnes colonization of the human shoulder.propionibacterium acnes (p. acnes) is frequently cultured in patients with wound infections after shoulder surgery. the purpose of this study was to characterize the colonization of various anatomic locations with p. acnes in order to explain this clinical observation.200919362854
cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms.in the course of this biodiversity study, the cultivable microbial community of european spacecraft-associated clean rooms and the herschel space observatory located therein were analyzed during routine assembly operations. here, we focused on microorganisms capable of growing without oxygen. anaerobes play a significant role in planetary protection considerations since extraterrestrial environments like mars probably do not provide enough oxygen for fully aerobic microbial growth. a broad assor ...200919363082
changing the face of acne therapy.many innovations in acne therapy have evolved since the discovery in 1949 that vitamin a derivatives affected epidermal proliferation. approval of topical tretinoin solution in 1971 was followed by modifications in the formulation to improve tolerability and provide flexibility in dosing. identification of retinoid receptors led to research that resulted in 2 receptor-selective synthetic retinoids: adapalene and tazarotene. today, topical retinoids are one of the cornerstones of acne therapy and ...200919363873
a previously uncultured, paper mill propionibacterium is able to degrade o-aryl alkyl ethers and various aromatic hydrocarbons.a previously uncultured propionibacterium was isolated from a highly diluted sample (10(-6)ml) of activated sludge of paper mill effluent. the isolate mob600 was able to grow on anisole, phenetole, benzene, toluene, phenol, styrene and biphenyl, although it used only limited carbon sources in the minimal media. the partial dna sequence of 16s ribosomal rna gene was 93% identical to luteococcus peritoni ccug38120 as the closest neighborhood in the family propionibacteriaceae. strain mob600 produc ...200919375147
increased interferon-gamma, interleukin-12p40 and il-8 production in propionibacterium acnes-treated peripheral blood mononuclear cells from patient with acne vulgaris: host response but not bacterial species is the determinant factor of the disease.acne vulgaris is a multifactorial inflammatory disease of the sebaceous follicles of the face and torso that frequently occurs in adolescence. initially, acne starts as a non-inflammatory comedo. subsequently, inflammatory reactions evolve to pustules, granulomas and cystic lesions. many pathogenic mechanisms have been proposed including sebum excretion, obstruction of hair follicles, impaired keratinization of hair epithelium, bacterial overgrowth and immunological mechanisms; the role of propi ...200919375895
characterization of beta-1,3-galactosyl-n-acetylhexosamine phosphorylase from propionibacterium acnes.homologs of the beta-1,3-galactosyl-n-acetylhexosamine phosphorylase (galhexnacp) gene (gnpa) were cloned from the genomic dna of propionibacterium acnes jcm6425 and p. acnes jcm6473, showing 99.9% and 97.9% nucleotide sequence identity, respectively, with the ppa0083 gene from the genome-sequenced p. acnes kpa171202. no gnpa gene was detected in the genomic dna of type strain p. acnes atcc25746. the recombinant enzyme from p. acnes jcm6425 (gnpa) showed approximately 70 times higher specific ac ...200919132369
probiotics prevent ige-associated allergy until age 5 years in cesarean-delivered children but not in the total cohort.less microbial exposure in early childhood is associated with more allergic disease later. allergic children have a different fecal microflora, with less lactobacilli and bifidobacteria. beneficial effects regarding the development of allergy have been suggested to come through probiotic supplementation.200919135235
in vitro anti-adhesive activity of green tea extract against pathogen adhesion.camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. the present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. green tea extract-4 (csi-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. it showed strong inhibitory activities against hemagglutination ...200919107860
mechanism of conjugated linoleic acid and vaccenic acid formation in human faecal suspensions and pure cultures of intestinal bacteria.faecal bacteria from four human donors and six species of human intestinal bacteria known to metabolize linoleic acid (la) were incubated with la in deuterium oxide-enriched medium to investigate the mechanisms of conjugated linoleic acid (cla) and vaccenic acid (va) formation. the main cla products in faecal suspensions, rumenic acid (cis-9,trans-11-cla; ra) and trans-9,trans-11-cla, were labelled at c-13, as were other 9,11 geometric isomers. traces of trans-10,cis-12-cla formed were labelled ...200919118369
in vitro activity of nadifloxacin against several gram-positive bacteria and analysis of the possible evolution of resistance after 2 years of use in germany.nadifloxacin has good activity against propionibacterium acnes as well as against both meticillin-susceptible and -resistant staphylococcus aureus (mssa and mrsa, respectively) and staphylococcus epidermidis. the aim of this study was to evaluate the activity of this fluoroquinolone against the abovementioned microorganisms, comparing isolates collected in 2007 in germany, a country where nadifloxacin has been used for the last 2 years, with isolates collected from 2006-2007 in spain where nadif ...200919095414
secondary infections of thoracic and abdominal aortic endografts.to review several cases of stent-graft infection with respective outcomes to identify clinical presentations and responses to treatment options.200919097807
dairy bacteria remove in vitro dietary lectins with toxic effects on colonic cells.to assess in vitro the ability of some dairy bacteria to bind concanavalin a (con a), peanut agglutinin (pna) and jacalin (ail), preventing their toxicity on mouse intestinal epithelial cells (iec).200919191953
expression, purification, crystallization and preliminary x-ray diffraction analysis of the soluble domain of ppa0092, a putative nitrite reductase from propionibacterium acnes.the soluble domain (residues 483-913) of ppa0092, a putative copper-containing nitrite reductase from propionibacterium acnes kpa171202, has been overexpressed in escherichia coli. the purified recombinant protein was crystallized using the hanging-drop vapour-diffusion method. x-ray diffraction data were collected and processed to a maximum resolution of 2.4 a. the crystal belonged to space group p2(1)3, with unit-cell parameters a = b = c = 108.63 a. preliminary diffraction data show that one ...200919194001
microbial quality of equine frozen semen.bacteriological surveillance is little applied in management of equine frozen semen but it is quite important to verify the microbial contamination in order to find out the chance of transmission of pathology to the mare in ai. authors describe a qualitative and quantitative analysis for bacterial contamination on long time (3-17 years) equine frozen semen stored in liquid nitrogen. the semen checked, produced in italy and in another europe country, was cryopreserved in liquid nitrogen inside se ...200919144477
the antibacterial activity of clove essential oil against propionibacterium acnes and its mechanism of action. 200919153353
expression of toll-like receptor 2 on peripheral blood monocytes of patients with inflammatory and noninflammatory acne vulgaris.the pathogenesis of acne vulgaris is multifactorial and entails the interplay of hormonal, microbial and immunological events. the bacterium propionibacterium acnes is involved in the induction of comedogenesis and maintenance of the inflammatory phase of acne. toll-like receptor 2 (tlr2) expressed on mononuclear inflammatory cells and possibly on keratinocytes and sebocytes is thought to be of vital importance in mediating p. acnes-induced inflammatory response in acne vulgaris. this work aimed ...200920726329
is there evidence for a role of propionibacterium acnes in prostatic disease? 200918407335
clinical considerations in the treatment of acne vulgaris and other inflammatory skin disorders: a status report.this article reviews the anti-inflammatory and nonantimicrobial effects of antibiotics in acne and other diseases and examines issues relating to the emergence of decreased bacterial sensitivity to antibiotics and how these issues relate to clinical practice. it includes an overview of the inflammatory activities of some antibiotic agents and their potential for use in various dermatologic and nondermatologic diseases. it demonstrates that p. acnes-resistant organisms may be associated with ther ...200918984363
optimizing use of oral antibiotics in acne vulgaris.oral antibiotics are commonly used to treat acne vulgaris, primarily in patients presenting with moderate to severe facial or truncal disease severity. these agents are most appropriately used in combination with a topical regimen containing benzoyl peroxide and a topical retinoid. the most common oral antibiotics for treating acne vulgaris are the tetracycline derivatives, although macrolide agents such as erythromycin have also been used extensively. over the past 4 decades, as the sensitivity ...200918984366
rapid detection and quantification of propionibacteriaceae.propionibacteriaceae (propioni) are anaerobic bacteria associated with human and animal infections. present-day methods of diagnosis for propioni are unsatisfactory due to a lack of sensitivity of culture, time required for culture results (3 to 14 days) and difficulties in interpreting sybr green real-time pcr results. the goal of this work was to validate a new rapid and sensitive test for the diagnosis of propioni infections (endophthalmitis, corneal ulcers and others). material and methods: ...200918977791
effectiveness of the antibiotic lock therapy for the treatment of port-related enterococci, gram-negative, or gram-positive bacilli bloodstream infections.the purpose of this study is to evaluate the efficacy of antibiotic lock therapy to treat port-related enterococci, gram-negative, or gram-positive bacilli bloodstream infections.200919026506
characterization of propionibacterium acnes isolates from sarcoid and non-sarcoid tissues with special reference to cell invasiveness, serotype, and trigger factor gene polymorphism.sarcoidosis is a systemic granulomatous disease of unknown etiology. propionibacterium acnes is the only microorganism so far isolated from sarcoid lesions. to examine whether p. acnes isolates from sarcoid tissues differ from those obtained from non-sarcoid tissues, we studied cell invasiveness, serotype, and polymorphisms of the p. acnes trigger factor protein and the two invasion-associated proteins (named pamce and pap60) in 35 p. acnes isolates from sarcoid lymph nodes and 127 isolates from ...200919049821
the prevalence and pathogenicity of propionibacterium acnes keratitis.to study the prevalence, pathogenicity, and virulence of propionibacterium acnes keratitis.200919092402
"sapho syndrome and infections".the syndrome of synovitis, acne, pustulosis, hyperostosis, osteitis (sapho) encompasses a broad spectrum of cutaneous manifestations associated with osteitic and hyperostotic lesions, which typically may involve the anterior chest wall (acw). the aetiopathogenetic mechanisms as well as the nosographic framing of the disease are still not fully defined although an important role has been suggested for propionibacterium acnes (p. acnes). this germ might be able to stimulate both the innate and the ...200918721907
what is the significance of a positive propionibacterium acnes culture around a joint replacement?the purpose of this study was to show the significance of a positive propionibacterium acnes sample around a joint replacement. records from the microbiology laboratory data over a 3-year period were reviewed to identify patients with prosthetic joints from whom propionibacterium acnes was isolated at least once. the medical records of all those patients were retrieved and the demographic, clinical, microbiological and haematological data were collected and examined. the preoperative values of e ...200918327582
kinetic analysis and ph-shift control strategy for propionic acid production with propionibacterium freudenreichii cctcc m207015.the production of propionic acid by propionibacterium freudenreichii cctcc m207015 was investigated in a 7.5-l stirred-tank fermentor. batch fermentations by p. freudenreichii cctcc m207015 at various ph values ranging from 5.5 to 7.0 were studied. based on the analysis of the time course of specific cell growth rate (mu (x)) and specific propionic acid formation rate (mu (p)), a two-stage ph-shift control strategy was proposed. at first 48 h, ph was controlled at 6.5 to obtain the maximal mu (x ...201018626579
activities of ten essential oils towards propionibacterium acnes and pc-3, a-549 and mcf-7 cancer cells.ten essential oils, namely, mint (mentha spicata l., lamiaceae), ginger (zingiber officinale rosc., zingiberaceae), lemon (citrus limon burm.f., rutaceae), grapefruit (citrus paradisi macf., rutaceae), jasmine (jasminum grandiflora l., oleaceae), lavender (mill., lamiaceae), chamomile (matricaria chamomilla l., compositae), thyme (thymus vulgaris l., lamiaceae), rose (rosa damascena mill., rosaceae) and cinnamon (cinnamomum zeylanicum n. lauraceae) were tested for their antibacterial activities ...201020657472
the complete genome of propionibacterium freudenreichii cirm-bia1, a hardy actinobacterium with food and probiotic applications.propionibacterium freudenreichii is essential as a ripening culture in swiss-type cheeses and is also considered for its probiotic use. this species exhibits slow growth, low nutritional requirements, and hardiness in many habitats. it belongs to the taxonomic group of dairy propionibacteria, in contrast to the cutaneous species p. acnes. the genome of the type strain, p. freudenreichii subsp. shermanii cirm-bia1 (cip 103027(t)), was sequenced with an 11-fold coverage.201020668525
innate, antigen-independent role for t cells in the activation of the immune system by propionibacterium acnes.propionibacterium acnes is a human commensal but also an opportunistic pathogen. in mice, p. acnes exerts strong immunomodulatory activities, including formation of intrahepatic granulomas and induction of lps hypersensitivity. these activities are dependent on p. acnes recognition via tlr9 and subsequent il-12-mediated ifn-gamma production. we show that p. acnes elicits il-12p40 and p35 mrna expression in macrophages, and ifn-gamma mrna in liver cd4(+) t cells and nk cells. after priming with p ...201020690177
propionibacterium acnes and staphylococcus epidermidis isolated from refractory endodontic lesions are opportunistic pathogens.the predominant cultivable microbiota from 20 refractory endodontic lesions (9 with abscesses and 11 without abscesses) were determined, and propionibacterium acnes and staphylococcus epidermidis were among the most predominant organisms. the number of species identified from lesions with abscesses (14.1 ± 2.6) was significantly greater (p < 0.001) than the number from lesions without abscesses (7.4 ± 5.9). comparison of perioral isolates using repetitive extragenic palindromic pcr of the same s ...201020739494
taurine bromamine (taubr)--its role in immunity and new perspectives for clinical use.this review is an attempt to summarize our knowledge about taurine bromamine (taubr) properties, its role in innate immunity and its therapeutic potential.taubr and taurine chloramine (taucl) are major haloamines generated by eosinophils and neutrophils at a site of inflammation. both haloamines share anti-inflammatory and anti-oxidant properties. taubr, similarly to taucl, decreases the production of proinflammatory mediators. their anti-inflammatory and anti-oxidant activities are enhanced by ...201020804605
propionibacterium acnes activates the igf-1/igf-1r system in the epidermis and induces keratinocyte proliferation.propionibacterium acnes has a major role in the development of acne lesions. igf-1 stimulates the proliferation of keratinocytes via an activation of the igf-1 receptor (igf-1r). zinc has been proven to work efficiently against inflammatory acne and to modulate the igf-1 system. our objectives were to study the modulation of igf-1 and igf-1r expression by p. acnes extracts and to determine their modulation by zinc gluconate. in vivo, we analyzed biopsies of acne lesions and healthy skin, and in ...201020927124
proteomic identification of secreted proteins of propionibacterium acnes.the anaerobic gram-positive bacterium propionibacterium acnes is a human skin commensal that resides preferentially within sebaceous follicles; however, it also exhibits many traits of an opportunistic pathogen, playing roles in a variety of inflammatory diseases such as acne vulgaris. to date, the underlying disease-causing mechanisms remain ill-defined and knowledge of p. acnes virulence factors remains scarce. here, we identified proteins secreted during anaerobic cultivation of a range of sk ...201020799957
evaluation of the scanrdi(r) as a rapid alternative to the pharmacopoeial sterility test method: comparison of the limits of detection.two sterility test methods, the scanrdi® rapid sterility test and the united states pharmacopeia/european pharmacopoeia/japanese pharmacopoeia (usp/ep/jp) compendial sterility test, were compared with respect to the limits of detection for the presence of viable microorganisms in aqueous solutions at low inoculation levels. the scanrdi® system employs a combination of direct fluorescent labeling techniques and solid-phase laser scanning cytometry to rapidly enumerate viable microorganisms from a ...201021502036
benzoyl peroxide: enhancing antibiotic efficacy in acne management.benzoyl peroxide is one of the most widely used topical agents for acne. it has potent antibacterial and mild anti-inflammatory and comedolytic effects. to treat mild to moderate acne, it can be used alone or in combination with topical antibiotics and topical retinoids. the combination of benzoyl peroxide with either erythromycin or clindamycin is synergistic and well-tolerated. in more severe acne, when oral antibiotics are required, benzoyl peroxide can contribute to suppressing the emergence ...201021076800
effect of probiotic propionibacterium jensenii 702 supplementation on layer chicken performance.the effects of the probiotic, propionibacterium jensenii 702 (pj 702), supplementation on egg productivity, egg shell thickness, fatty acid profile of eggs, and body weight in early layer hens were investigated. twenty eight twenty-week-old starter pullets were evenly divided into a treatment and a control group for an eight week experiment. each bird in the treatment group received 107 cfu pj 702 daily in a total volume of 1 ml by oral administration. no adverse effect was observed due to admin ...201021831750
normal human epidermal keratinocytes react differently than hacat keratinocyte cell line on exposure to propionibacterium acnes. 201021352277
[how i explore...skin by photodiagnosis using skin fluorescence and its functional imaging].under specific light illumination, skin produces peculiar fluorescent aspects. such property can be used to provide diagnostic arguments or to disclose some peculiar functions of the skin. some superficial infections (erythrasma, tinea versicolor, dermatophytic tinea,..) reveal fluorescence that may be intense. the pilosebaceous follicles enriched in propionibacterium spp show fluorescence. such property is lost during some anti-acne treatments. a quenching effect is observed after application o ...201021086585
tlr2 expression is increased in rosacea and stimulates enhanced serine protease production by keratinocytes.a diverse environment challenges skin to maintain temperature, hydration, and electrolyte balance while also maintaining normal immunological function. rosacea is a common skin disease that manifests unique inflammatory responses to normal environmental stimuli. we hypothesized that abnormal function of innate immune pattern recognition could explain the enhanced sensitivity of patients with rosacea, and observed that the epidermis of patients with rosacea expressed higher amounts of toll-like r ...201021107351
[evaluating post-cataract surgery changes in bacterial culture tests with and without face-washing].to evaluate the changes in conjunctiva and eyelid culture test results before and after cataract surgery and their relation to face-washing.201020865920
time course and specificity of lipolysis in swiss cheese.controlling lipolysis in cheese is necessary to ensure the formation of desirable flavor. to get a better understanding of the mechanism of lipolysis in swiss cheese, cheeses were manufactured with and without (control) the addition of propionibacterium freudenreichii. products of lipolysis were quantified throughout ripening. half of the free fatty acids (ffa) released in milk (3.66 mg/g fat), in particular the short-chain ffa, were lost in the whey during curd drainage, whereas diglycerides an ...201020961108
safety of high doses of propionibacterium freudenreichii et-3 culture in healthy adult subjects.propionibacterium freudenreichii et-3 (7025) culture, a cell-free product of whey fermentation by p. freudenreichii et-3, has been shown to promote the growth of bifidobacteria through the action of 1,4-dihydroxy-2-naphthoic acid (dhna). here we report the results of two clinical studies designed to evaluate the safety of high doses of p. freudenreichii et-3 culture medium. study 1 had a randomized, double-blind, crossover design. ten healthy male and four healthy female subjects received 45 tab ...201021172397
staphylococcus aureus hijacks a skin commensal to intensify its virulence: immunization targeting β-hemolysin and camp factor.the need for a new anti-staphylococcus aureus therapy that can effectively cripple bacterial infection, neutralize secretory virulence factors, and lower the risk of creating bacterial resistance is undisputed. here, we propose what is, to our knowledge, a previously unreported infectious mechanism by which s. aureus may commandeer propionibacterium acnes, a key member of the human skin microbiome, to spread its invasion and highlight two secretory virulence factors (s. aureus β-hemolysin and p. ...201021085191
antibiotic susceptibility of propionibacterium acnes isolated from acne vulgaris in korea.propionibacterium acnes plays an important role in the development of acne, and inflammatory lesions are improved by antibiotics. long-term use of antibiotics may result in development of resistant strains and treatment failure. the aim of the present study was to investigate the isolation rate of p. acnes and to evaluate its antibiotic susceptibility to widely used antibiotics in acne in korea. among 46 patients, 31 p. acnes strains were cultured. isolated p. acnes was measured for minimum inhi ...201021352337
efficacy of oral antibiotics on acne vulgaris and their effects on quality of life: a multicenter randomized controlled trial using minocycline, roxithromycin and faropenem.there are few clinical studies which compare the efficacy and patient satisfaction for oral antibiotics to treat inflammatory acne. to clarify the difference between oral antibiotics, acne patients with moderate to severe inflammatory eruptions were randomized into three groups, and each patient was given minocycline (mino), roxithromycin (rxm) or faropenem (frpm) for 4 weeks, followed by 4 weeks of observation without any oral antibiotics. we estimated the reduction rate of inflammatory lesion ...201021269305
propionibacterium acnes endogenous endophthalmitis presenting with bilateral scleritis and uveitis. 201019648894
identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach.this study identified the bacterial taxa enduring endodontic treatment procedures by using a combined 16s ribosomal rna-based reverse-transcriptase polymerase chain reaction (rt-pcr) and reverse-capture checkerboard hybridization approach.201020003934
tibial chronic osteomyelitis due to propionibacterium acnes in a patient with sickle cell anemia. 201019913448
in vitro antimicrobial activity of silver-processed catheters for neurosurgery.to investigate the in vitro antibacterial activity of silver-processed catheters for use in neurosurgery using clinically predictive tests.201019942617
an in vitro study on bacterial growth interactions and intestinal epithelial cell adhesion characteristics of probiotic combinations.the aims of this study were to examine long-term growth interactions of five probiotic strains (lactobacillus casei 01, lactobacillus plantarum ha8, lactobacillus rhamnosus gg, lactobacillus reuteri atcc 55730 and bifidobacterium lactis bb12) either alone or in combination with propionibacterium jensenii 702 in a co-culture system and to determine their adhesion ability to human colon adenocarcinoma cell line caco-2. growth patterns of probiotic lactobacillus strains were not considerably affect ...201019949794
antimicrobial activity and stability of rhinacanthins-rich rhinacanthus nasutus extract.rhinacanthins-rich rhinacanthus nasutus (rrn) extract was prepared and standardized to contain total rhinacantins not less than 70% w/w. in this study, antimicrobial activities of the rrn extract as well as rhinacanthin-c against streptococcus mutans, propionibacterium acnes, helicobacter pylori, staphylococcus aureus, s. epidermidis and candida albicans were evaluated by microdilution assay. it was found that the rrn extract exhibited potent bactericidal activity against s. mutans with mic and ...201019879741
18beta-glycyrrhetinic acid ameliorates acute propionibacterium acnes-induced liver injury through inhibition of macrophage inflammatory protein-1alpha.18beta-glycyrrhetinic acid (ga), the major bioactive component of licorice root extract, has a protective effect on hepatic injury and exhibits antiinflammatory activity. here, we investigate the effect of ga in propionibacterium acnes-induced acute inflammatory liver injury. c57bl/6 mice were primed with p. acnes followed by lipopolysaccharide challenge to induce fulminant hepatitis. ga (75 mg/kg) or vehicle control was administered intraperitoneally daily 1 day after p. acnes priming, and ga s ...201019897483
propionibacterium acnes infection after shoulder arthroplasty: a diagnostic challenge.this study reviewed a series of patients diagnosed with propionibacterium acnes infection after shoulder arthroplasty in order to describe its clinical presentation, the means of diagnosis, and provide options for treatment.201019884021
suppressive effects of 1,4-dihydroxy-2-naphthoic acid administration on bone resorption.the main component of the metabolic by-products of fermentation by propionibacterium freudenreichii et-3 is 1,4-dihydroxy-2-naphthoic acid (dhna), which has a naphthoquinone skeleton, as in vitamin k2. this study showed that dhna improved bone mass reduction with osteoporosis model mice caused by fk506.201019813044
rapid identification of propionibacterium acnes from blood cultures by fluorescence in situ hybridization.a newly designed probe for rapid identification of propionibacterium acnes by fluorescence in situ hybridization was evaluated using 111 isolates from subculture and showed 100% sensitivity and specificity. a sensitivity of 95% and a specificity of 100% were achieved with direct application on 55 blood cultures containing gram-positive rods.201019815363
effect of konjac glucomannan hydrolysates and probiotics on the growth of the skin bacterium propionibacterium acnes in vitro.the synbiotic ability of probiotic bacteria and konjac glucomannan hydrolysates (gmh) to inhibit acne-inducing bacterium, propionibacterium acnes growth was studied in vitro. all probiotic bacteria strains tested were able to inhibit the growth of this species of skin bacterium where the inhibition was significantly (p < 0.01) enhanced by the presence of the gmh prebiotic. as the current treatment of acne is based on topical or systemic drugs, it is worth examining further the biotherapeutic act ...201019818083
microbiological examination of donated human cardiac tissue in heart valve banking.microbiological examination of donated human cardiac tissue is a necessary procedure for heart valve banks to determine the biological safety of preserved allografts. test protocols must be validated to prevent false-negative outcomes that pose a risk of infection to recipients of the tissue. the heart valve bank in rotterdam evaluated a validated, alternative entry test for donated tissues to compare the performance of its standard microbiological examinations.201019709896
spondylodiscitis due to propionibacterium acnes: report of twenty-nine cases and a review of the literature.propionibacterium acnes is the most frequent anaerobic pathogen found in spondylodiscitis. a documented case required microbiological proof of p. acnes with clinical and radiological confirmation of inflammation in a localized region of the spine. microbiological samplings were obtained by surgery or aspiration under radiological control. twelve males and 17 females (median age, 42 years) with spondylodiscitis due to p. acnes were diagnosed within the last 15 years. three patients were immunosup ...201019519850
photodynamic therapy of acne vulgaris using 5-aminolevulinic acid 0.5% liposomal spray and intense pulsed light in combination with topical keratolytic agents.increasing antibiotic resistance of propionibacterium acnes and growing awareness on the side effects of topical and systemic drugs in the treatment of acne vulgaris by physicians and patients have paved the way for a search into new efficacious and safe treatment modalities such as photodynamic therapy (pdt). although the efficacy of pdt using 20% 5-aminolevulinic acid (ala) cream has been established, phototoxic side effects limit its use. the 5-ala concentration can be lowered by a factor of ...201019796088
interleukin-10 secretion from cd14+ peripheral blood mononuclear cells is downregulated in patients with acne vulgaris.acne is a common chronic inflammatory dermatosis of the pilosebaceous unit. it is characterized by seborrhoea, comedone formation and an inflammatory response consistent with defective cellular immunity to propionibacterium acnes.201019796181
propionic acid fermentation by propionibacterium freudenreichii cctcc m207015 in a multi-point fibrous-bed bioreactor.propionic acid was produced in a multi-point fibrous-bed (mfb) bioreactor by propionibacterium freudenreichii cctcc m207015. the mfb bioreactor, comprising spiral cotton fiber packed in a modified 7.5-l bioreactor, was effective for cell-immobilized propionic acid production compared with conventional free cell fermentation. batch fermentations at various glucose concentrations were investigated in the mfb bioreactor. based on analysis of the time course of production, a fed-batch strategy was a ...201020589397
treatment of progressive macular hypomelanosis with narrow-band ultraviolet b phototherapy.treatment for progressive macular hypomelanosis (pmh) has been disappointing. recently, propionibacterium acnes had been postulated as the causative agent and narrow-band ultraviolet b (nbuvb) had been shown to stimulate melanogenesis and has antibacterial properties. the aim of this study was to evaluate the effectiveness of nbuvb in the treatment of pmh. a retrospective analysis of pmh cases diagnosed and treated with nbuvb treatment from 1 january 2007 to 30 april 2009 at the national skin ce ...201020584255
randomized tolerability analysis of clindamycin phosphate 1.2%-tretinoin 0.025% gel used with benzoyl peroxide wash 4% for acne vulgaris.the multiple etiologic factors involved in acne vulgaris make the use of several medications necessary to treat the condition. use of a fixed combination of clindamycin phosphate 1.2% and tretinoin 0.025% in conjunction with a benzoyl peroxide (bpo) wash 4% targets several pathologic factors simultaneously and mitigates the potential for clindamycin-induced propionibacterium acnes-resistant strains. new formulations may allow such regimens to be effectively used without overly reduced tolerabili ...201021284283
multilocus sequence typing of propionibacterium freudenreichii.propionibacterium freudenreichii is used as a ripening culture in swiss cheese manufacture. this study investigates the molecular diversity and the population structure of this bacterium via multilocus sequence typing (mlst). internal fragments of seven genes sequenced for 113 strains of different subspecies and origins allowed the resolution of 46 sequence types (sts) with occurrence frequencies ranging from 1 to 11. the core genome of the species harbours a low level of nucleotide polymorphism ...201021176990
recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice.we have previously demonstrated that oral administration of a metabolically active bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (cla), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 cla and omega-3 (n-3) fatty acids in liver and adipose tissue. in this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production ...201021178166
synthesis and antimicrobial activity of long-chain 3,4-epoxy-2-alkanones.3,4-epoxy-2-dodecanone, a major component in the preorbital gland of the african grey duiker (sylvicapra grimmia), showed antimicrobial activity in preliminary tests. the c(11) to c(17) homologues of this compound were prepared and their activity against several pathogenic dermal bacteria and fungi was tested. 3,4-epoxy-2-dodecanone and 3,4-epoxy-2-tridecanone inhibited the growth of trichophyton mentagrophytes at 25 îg/ml. moderate inhibition of the growth of the bacteria propionibacterium acne ...201021179314
production of 5-aminolevulinic acid by propionibacterium acidipropionici tistr442.propionibacterium acidipropionici tistr442 produced the highest amount of 5-aminolevulinic acid (ala) when cultivated in medium supplemented with glycine at 18g/l. ala production correlated with ala synthase activity, whereas ala dehydratase activity was maintained at a low level. ala yield reached 405mg/l after prolonged cultivation for 1 month.201021185777
treating acne in women. 201021214119
acne epidemiology and pathophysiology.the demographic profile of facial acne vulgaris has changed during the past several decades; 12 years of age is no longer the low end of the "normal" range for onset of acne. the available epidemiologic evidence raises more questions than it answers regarding the etiology of this downward shift. more study is needed to clarify whether the trend toward an earlier onset of puberty in the united states has influenced the clinical picture of acne. additional research will help advance understanding ...201020610306
in vivo antimicrobial effects of endodontic treatment procedures as assessed by molecular microbiologic techniques.this culture-independent molecular microbiology study evaluated the antimicrobial effects of chemomechanical preparation supplemented by intracanal medication during treatment of teeth with apical periodontitis.201021329812
glycerol enhances the antifungal activity of dairy propionibacteria.dairy propionibacteria are widely used in starter cultures for swiss type cheese. these bacteria can ferment glucose, lactic acid, and glycerol into propionic acid, acetic acid, and carbon dioxide. this research examined the antifungal effect of dairy propionibacteria when glycerol was used as carbon source for bacterial growth. five type strains of propionibacteria were tested against the yeast rhodotorula mucilaginosa and the molds penicillium commune and penicillium roqueforti. the conversion ...201021331381
optimization and scale-up of propionic acid production by propionic acid-tolerant propionibacterium acidipropionici with glycerol as the carbon source.propionic acid production by propionibacterium acidipropionici with glycerol as sole carbon source was studied in 7-l batch and 10m(3) bioreactor cultures. in batch cultures, propionic acid production increased and specific cell growth rate decreased with increasing glycerol concentrations. maximum propionic acid production and productivity reached 44.62+/-1.12g/l and 0.20+/-0.0075gl(-1)h(-1)at 220h, respectively, when glycerol was fed at a constant rate of 0.01l/h from 72 to 120h with an initia ...201020620054
comparative study of the bactericidal effects of 5-aminolevulinic acid with blue and red light on propionibacterium acnes.propionibacterium acnes naturally produces endogenous porphyrins that are composed of coproporphyrin iii (cpiii) and protoporphyrin ix (ppix). red light alone and photodynamic therapy (pdt) improve acne vulgaris clinically, but there remains a paucity of quantitative data that directly examine the bactericidal effects that result from pdt on p. acnes itself in vitro. the purpose of this study was to measure the difference of bactericidal effects of 5-aminolevulinic acid (ala)-pdt with red and bl ...201021352326
the tlr4/trif-mediated activation of nlrp3 inflammasome underlies endotoxin-induced liver injury in mice.administration of heat-killed propionibacterium acnes renders mice highly susceptible to lps. after lps challenge p. acnes-primed mice promptly show hypothermia, hypercoagulation (disseminated intravascular coagulation), elevation of serum proinflammatory cytokine levels, and high mortality. the surviving mice develop liver injury. as previously reported, il-18 plays a pivotal role in the development of this liver injury. many cell types including macrophages constitutively store il-18 as biolog ...201020634907
antibacterial activity of thai herbal extracts on acne involved microorganism.ethyl acetate and methanol extracts of 18 thai medicinal plants were investigated for their antibacterial activity against propionibacterium acnes, stapylococcus aureus, and s. epidermidis. thirteen plant extracts were capable of inhibiting the growth of p. acnes and s. epidermidis, while 14 plant extracts exhibited an inhibitory effect on s. aureus. based on the broth dilution method, the ethyl acetate extract of alpinia galanga (l.) wild. (zingiberaceae) rhizome showed the strongest antibacter ...201020645714
in vitro antimicrobial susceptibility of propionibacterium acnes isolated from acne patients in northern mexico.antimicrobials are essential in acne therapy. in the last decades, propionibacterium acnes has become resistant to different antibiotics.201020931669
prevalence of propionibacterium acnes in diseased prostates and its inflammatory and transforming activity on prostate epithelial cells.prostate cancer (pca) is the second leading cause of male cancer deaths in the western world. mounting evidence has revealed that chronic inflammation can be an important initiating factor of pca. recent work has detected the anaerobic gram-positive bacterium propionibacterium acnes in cancerous prostates, but with wide-ranging detection rates. here, using in situ immunofluorescence (isif), p. acnes was found in 58 out of 71 (81.7%) tested cancerous prostate tissue samples, but was absent from h ...201020943438
fermentation metabolites from lactobacillus gasseri and propionibacterium freudenreichii exert bacteriocidal effects in mice.lactobacillus gasseri oll 2716 promotes the elimination of helicobacter pylori and is utilized in yogurts that are specifically labeled as health foods. on the other hand, milk whey fermented by propionibacterium freudenreichii et-3, which increases the numbers of bifidobacterium, is effective for intestinal disorders. we previously demonstrated that oral administration of l. gasseri and p. freudenreichii fermentation metabolites (lp-fm) improved calf intestinal microflora and reduced the incide ...201020954806
infected giant ophthalmic artery aneurysm remnant following craniotomy for surgical clip ligation. case report.postoperative intracranial infections, although found in only a minority of surgical cases, remain a recognized potential complication following elective craniotomy. in the treatment of intracranial aneurysms, specifically, reports of significant postoperative infections are rare. significant postoperative infections are usually observed in association with foreign bodies, such as aneurysm clips, endovascular coils, or materials used for aneurysm wrapping. the authors present a case in which a p ...201020345224
propioniciclava tarda gen. nov., sp. nov., isolated from a methanogenic reactor treating waste from cattle farms.facultatively anaerobic bacterial strains (wr061(t)and wr054) were isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms in japan. the two strains had almost the same phylogenetic and phenotypic properties. cells were gram-staining-positive, non-motile, non-spore-forming, irregular rods. the strains required cobalamin (vitamin b(12)). the strains utilized various carbohydrates including hexoses and disaccharides. the strains produced acetate and propionate f ...201020971831
promising immunomodulatory effects of selected strains of dairy propionibacteria as evidenced in vitro and in vivo.immunomodulatory properties of 10 dairy propionibacteria, analyzed on human peripheral blood mononuclear cells (pbmcs), revealed a highly strain-dependent induction of anti-inflammatory cytokine interleukin 10 (il-10). two selected strains of propionibacterium freudenreichii showed a protective effect against two models of colitis in mice, suggesting a probiotic potential predicted by immune-based selection criteria for these cheese starter bacteria.201020971874
bacterial contamination of needles used for intravitreal injections: a prospective, multicenter study.to determine the incidence of bacterial contamination of needles used for intravitreal injections.201021034310
mis416, a non-toxic microparticle adjuvant derived from propionibacterium acnes comprising immunostimulatory muramyl dipeptide and bacterial dna promotes cross-priming and th1 immunity.propionibacterium acnes was modified using biochemical extraction methods generating a suspension of microparticles (mis416) comprising a minimal cell wall skeleton rich in immunostimulatory crosslinked muramyl dipeptide repeats and native bacterial dna fragments, each which have known adjuvant activity. in vitro studies demonstrated that mis416 was readily internalized by human myeloid and plasmacytoid dc inducing cytokine secretion and cell activation/maturation. vaccination studies in mice us ...201021034827
the unconventional antimicrobial peptides of the classical propionibacteria.the classical propionibacteria produce genetically unique antimicrobial peptides, whose biological activities are without equivalents, and to which there are no homologous sequences in public databases. in this review, we summarize the genetics, biochemistry, biosynthesis, and biological activities of three extensively studied antimicrobial peptides from propionibacteria. the propionicin t1 peptide constitutes a bona fide example of an unmodified general secretory pathway (sec)-dependent bacteri ...201021038096
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