Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| crystal structure of the bczbp, a zinc-binding protein from bacillus cereus. | bacillus cereus is an opportunistic pathogenic bacterium closely related to bacillus anthracis, the causative agent of anthrax in mammals. a significant portion of the b. cereus chromosomal genes are common to b. anthracis, including genes which in b. anthracis code for putative virulence and surface proteins. b. cereus thus provides a convenient model organism for studying proteins potentially associated with the pathogenicity of the highly infectious b. anthracis. the zinc-binding protein of b ... | 2007 | 17501983 |
| simultaneous removal of chlorothalonil and nitrate by bacillus cereus strain ns1. | elevated no3- and chlorothalonil (ctn) have been found in production nursery recycling ponds. bacillus cereus strain ns1 isolated from nursery recycling pond sediment was assessed for its ability to reduce no3- and degrade ctn in a mineral medium. the results showed that the efficiency of no3- reduction and ctn degradation by b. cereus strain ns1 were related to the nature of organic carbon sources added to the medium. in the medium amended with 100 mg/l yeast extract, 86% of no3- (100 mg/l) and ... | 2007 | 17509667 |
| food poisoning due to jimson weed mimicking bacillus cereus food intoxication in austria, 2006. | 2007 | 17512772 | |
| toxin production in a rare and genetically remote cluster of strains of the bacillus cereus group. | three enterotoxins are implicated in diarrhoeal food poisoning due to bacillus cereus: haemolysin bl (hbl), non-haemolytic enterotoxin (nhe), and cytotoxin k (cytk). toxin gene profiling and assays for detection of toxin-producing stains have been used in attempts to evaluate the enterotoxic potential of b. cereus group strains. b. cereus strain nvh 391/98, isolated from a case of fatal enteritis, was genetically remote from other b. cereus group strains. this strain lacked the genes encoding hb ... | 2007 | 17517121 |
| bacillus cereus spores during housing of dairy cows: factors affecting contamination of raw milk. | the contamination of raw milk with bacillus cereus spores was studied during the indoor confinement of dairy cattle. the occurrence of spores in fresh and used bedding material, air samples, feed, feces, and the rinse water from milking equipment was compared with the spore level in bulk tank milk on 2 farms, one of which had 2 different housing systems. a less extensive study was carried out on an additional 5 farms. high spore concentrations of >100 spores/l in the raw milk were found on 4 of ... | 2007 | 17517714 |
| two distinct manganese-containing superoxide dismutase genes in bacillus cereus: their physiological characterizations and roles in surviving in wheat rhizosphere. | rhizosphere inhabitants interact intricately with plant host. bacillus cereus 905 isolated from wheat rhizosphere colonized wheat rhizosphere with large population size. in this work, the role of superoxide dismutases (sods) of b. cereus 905 in surviving in wheat rhizosphere was analyzed. two genes, soda-1 and soda-2 encoding two distinct manganese sods (mnsods), were isolated from the bacterium. the amino acid sequence similarity between the two peptides is 58.43%. through homologous recombinat ... | 2007 | 17521361 |
| [bacillus cereus enterotoxins]. | data about bacillus cereus different enterotoxins including hemolysin (hbl), nonhemolytic enterotoxin (nhe), enterotoxin (t), and emesis-inducing thermostable enterotoxin (ete) are summarized in the article. information about synthesis of different diarrhea-inducing and emesis-inducing enterotoxins, methods of their purification, structure, functions, and mechanisms of action are presented. commercial kits for identification of b. cereus enterotoxins causing food-associated diarrhea are listed. | 2007 | 17523492 |
| phenotypic and functional characterization of bacillus anthracis biofilms. | biofilms, communities of micro-organisms attached to a surface, are responsible for many chronic diseases and are often associated with environmental reservoirs or lifestyles. bacillus anthracis is a gram-positive, endospore-forming bacterium and is the aetiological agent of pulmonary, gastrointestinal and cutaneous anthrax. anthrax infections are part of the natural lifecycle of many ruminants in north america, including cattle and bison, and b. anthracis is thought to be a central part of this ... | 2007 | 17526827 |
| large-scale production and efficient recovery of phb with desirable material properties, from the newly characterised bacillus cereus spv. | a newly characterised bacillus strain, bacillus cereus spv was found to produce phb at a concentration of 38% of its dry cell weight in shaken flask cultures, using glucose as the main carbon source. polymer production was then scaled up to 20 l batch fermentations where 29% dry cell weight of phb was obtained within 48 h. following this, a simple glucose feeding strategy was developed and the cells accumulated 38% dry cell weight of phb, an increase in the overall volumetric yield by 31% compar ... | 2007 | 17532079 |
| differentiation and characterization by molecular techniques of bacillus cereus group isolates from poto poto and dégué, two traditional cereal-based fermented foods of burkina faso and republic of congo. | poto poto (a maize sourdough) and dégué (a pearl millet-based food) are two traditional african fermented foods. the molecular biology of toxigenic and pathogenic bacteria found in those foods is largely unknown. the purpose of this study was to study the phylogenetic relatedness and toxigenic potential of 26 bacillus cereus group isolates from these traditional fermented foods. the relatedness of the isolates was evaluated with repetitive element sequence-based pcr (rep-pcr) and 16s rdna sequen ... | 2007 | 17536675 |
| a novel antibiotic bone assay by liquid chromatography/tandem mass spectrometry for quantitation of tigecycline in rat bone. | tigecycline (tygacil) is a first-in-class, broad spectrum antibiotic with activity against antibiotic-resistant organisms. in rats and humans, tigecycline readily distributes to bone tissue but its accuracy of quantitation via liquid chromatography/mass spectrometry (lc/ms/ms) is hindered by a low extraction recovery when using a conventional plasma extraction method. to overcome this issue, we have identified an effective extraction solvent for quantitation of tigecycline in rat bone. the curre ... | 2007 | 17537611 |
| genomic diversity and relationship of bacillus thuringiensis and bacillus cereus by multi-rep-pcr fingerprinting. | the genomic diversity and relationship among 56 bacillus thuringiensis and bacillus cereus type strains were investigated by multi-rep-pcr fingerprinting consisting of three pcr reactions targeting the enterobacterial eric1 and eric2 and the streptococcal boxa1r consensus sequences. a total of 113 polymorphic bands were generated in the rep-pcr profiles that allowed tracing of a single dendrogram with three major groups. bacillus cereus strains clustered together in the a and b groups. most of t ... | 2007 | 17538643 |
| mycorrhizal fungi and ectomycorrhiza associated bacteria isolated from an industrial desert soil protect pine seedlings against cd(ii) impact. | effects of mycorrhization with amanita rubescens or hebeloma sinapizans and dual inoculation with the fungi and ectomycorrhiza associated bacteria (emab) pseudomonas putida or bacillus cereus on seedling growth and accumulation of cd(ii) in pinus sylvestris were studied. both fungal and bacterial species were isolated from roots of pines growing in an industrial area polluted with high concentrations of heavy metals. during mycorrhization, a. rubescens colonized higher number of pine seedlings t ... | 2007 | 17541824 |
| antibacterial activity of extract, fractions and four compounds extracted from piper solmsianum c. dc. var. solmsianum (piperaceae). | piper solmsianum c. dc. var. solmsianum (piperaceae) is a shrub commonly found in areas with wet tropical soils. other piper species have been used in folk medicine as antitumoral and antiseptic agents. we studied the crude methanolic extract, some organic fractions and compounds isolated from this plant for possible antimicrobial activity against gram-positive and gram-negative bacteria. the bioautographic assays disclosed three inhibition zones. the minimal inhibitory concentration (mic) and m ... | 2007 | 17542481 |
| synthesis, characterization and antimicrobial activity of new aliphatic sulfonamide. | a series of novel aliphatic sulfonamide derivatives (1-7) were synthesized and characterized by elemental analyses, ft-ir, (1)h nmr, (13)c nmr and lc-ms techniques. all the synthesized compounds were evaluated in vitro as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, bacillus cereus atcc 6633 and listeria monocytogenes atcc li6 (isolate), gram-negative bacteria (escherichia coli atcc 11230) and antifungal agent against candida albicans (c ... | 2007 | 17544281 |
| the contribution of gc1qr/p33 in infection and inflammation. | human gc1qr/p33 is a multi-compartmental and multi-functional cellular protein expressed on a wide range of tissues and cell types including lymphocytes, endothelial cells, dendritic cells, and platelets. although originally isolated as a receptor for c1q by virtue of its affinity (k(d)=15-50 nm), and specificity for the globular heads of this molecule, a large body of evidence has now been accumulated which shows that in addition to c1q, gc1qr can serve as a receptor for diverse proinflammatory ... | 2007 | 17544818 |
| quantitative analysis of population heterogeneity of the adaptive salt stress response and growth capacity of bacillus cereus atcc 14579. | bacterial populations can display heterogeneity with respect to both the adaptive stress response and growth capacity of individual cells. the growth dynamics of bacillus cereus atcc 14579 during mild and severe salt stress exposure were investigated for the population as a whole in liquid culture. to quantitatively assess the population heterogeneity of the stress response and growth capacity at a single-cell level, a direct imaging method was applied to monitor cells from the initial inoculum ... | 2007 | 17545319 |
| germination of bacillus cereus spores is induced by germinants from differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine. | spores of 11 enterotoxigenic strains of bacillus cereus isolated from foods and humans adhered with similar efficiencies to caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. notably, hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth. | 2007 | 17545321 |
| crystal structure of homoserine o-succinyltransferase from bacillus cereus at 2.4 a resolution. | 2007 | 17546672 | |
| influence of biofilm composition on the resistance to detachment. | bacillus cereus and pseudomonas fluorescens were used to develop monoculture biofilms in a bioreactor rotating system using a stainless steel cylinder for biofilm formation. the biofilms were allowed to grow for 7 days, exposed continuously to a reynolds number of agitation (rea) of 2,400. afterwards, the biofilms were characterised in terms of respiratory activity, amount of biomass, cellular density, cellular size and total and extracellular proteins and polysaccharides. the biofilm mechanical ... | 2007 | 17547019 |
| characterization of a small plcr-regulated gene co-expressed with cereolysin o. | in the human pathogen bacillus cereus, the expression of most extracellular virulence factors is controlled by the transcriptional activator plcr. among these virulence factors, cereolysin o (clo) is an haemolysin belonging to the cholesterol-dependant cytolysins, a protein family extensively studied in gram-positive bacteria. | 2007 | 17555563 |
| neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidant-dependent apoptosis in xenopus laevis oocytes. | ceramide regulates many cellular processes, including cell growth, differentiation, and apoptosis. although the effects of exogenous bacterial neutral sphingomyelinase (smase) in xenopus laevis oocytes have been investigated, its microinjection into oocytes has not been reported previously. thus, we compared the incubation versus microinjection of the neutral bacillus cereus sphingomyelinase (bsmase) to examine whether the topology of ceramide generation determines its effects on the fate of ooc ... | 2007 | 17556754 |
| synthesis and antibacterial activity of tert-butyl [1-benzyl-2[(4-aryl-2-thiazolyl)hydrazono]ethyl]carbamate derivatives. | the increasing clinical importance of drug-resistant fungal and bacterial pathogens has lent additional urgency to microbiological research and new antibacterial compound development. for this purpose, new tert-butyl[1-benzyl-2[(4-aryl-2-thiazolyl)hydrazono]ethyl]carbamate derivatives were synthesized and evaluated for antibacterial activity. the reaction of boc-l-phenylalaninal with thiosemicarbazide gave the thiosemicarbazone which furnished the title compounds by reaction with phenacyl bromid ... | 2007 | 17562564 |
| antibacterial properties and major bioactive components of cinnamon stick (cinnamomum burmannii): activity against foodborne pathogenic bacteria. | cinnamomum burmannii blume (cinnamon stick) from indonesia is a little-investigated spice. in this study, the antibacterial activity, minimum inhibitory concentration (mic), and minimum bactericidal concentration (mbc) of cinnamon stick extract were evaluated against five common foodborne pathogenic bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli, and salmonella anatum). cinnamon stick extract exhibited significant antibacterial properties. major compou ... | 2007 | 17567030 |
| microbiological detection of 10 quinolone antibiotic residues and its application to artificially contaminated poultry samples. | to assess if microbiological inhibition tests for detection of antibiotic residues are suitable for routine screening for quinolone residues, the limit of detection (lod) of 10 different quinolones and fluoroquinolones was determined. two media were tested, one at ph 6 and the other at ph 8, each seeded with one of the following test strains: bacillus subtilis, escherichia coli or bacillus cereus. lods of the 10 substances were highest on plates seeded with b. cereus, intended for selective dete ... | 2007 | 17364926 |
| antimicrobial activity and pollen composition of honey samples collected from different provinces in turkey. | the antibacterial activity of honey samples from different sources were collected and investigated against bacillus cereus, staphylococcus aureus atcc 25923, pseudomonas aeruginosa atcc 27853, klebsiella pneumoniae atcc 27736, morganella morganii, micrococcus luteus nrrl b-4375, escherichia coli atcc 35218, and candida albicans. pathogens exhibited different sensitivities towards the honey samples. the results showed that majority of the honey samples (75%) generally inhibitied the bacteria test ... | 2007 | 17365707 |
| bacillus cereus strain 10-l-2 produces two arylamine n-acetyltransferases that transform 4-phenylenediamine into 4-aminoacetanilide. | a bacterium, strain 10-l-2, that was isolated from soil and identified as bacillus cereus grew well on medium containing 4-phenylenediamine and polypepton. strain 10-l-2 converted a wide variety of anilines, including 4-phenylenediamine, to their corresponding acetanilides. growing cells acetylated a single amino group of 4-phenylenediamine to form 4-aminoacetanilide with a 97% molar yield, as shown by mass spectrometry and hplc. cell extracts exhibited arylamine n-acetyltransferase (nat) activi ... | 2007 | 17368397 |
| inactivation of foodborne pathogens using a one atmosphere uniform glow discharge plasma. | this study was conducted to determine the efficacy of a one atmosphere uniform glow discharge plasma (oaugdp) for inactivation of foodborne pathogens and to evaluate the influence of growth temperature, ph, and culture age on their inactivation. escherichia coli o157:h7, listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, vibrio parahaemolyticus, yersinia enterocolitica, and shigella flexneri were evaluated. three-strain mixtures of each bacterium were inocula ... | 2007 | 17378708 |
| a streamlined metabolic pathway for the biosynthesis of moenomycin a. | moenomycin a (mma) is a member of the phosphoglycolipid family of antibiotics, which are the only natural products known to directly target the extracellular peptidoglycan glycosyltransferases involved in bacterial cell wall biosynthesis. the structural and biological uniqueness of mma make it an attractive starting point for the development of new antibacterial drugs. in order both to elucidate the biosynthesis of this unusual compound and to develop tools to manipulate its structure, we have i ... | 2007 | 17379141 |
| culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data. | the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ... | 2007 | 17380356 |
| antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production. | to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti. | 2007 | 17381739 |
| characterization of a bacteriocin, thermophilin 1277, produced by streptococcus thermophilus sbt1277. | to assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, thermophilin 1277, production by streptococcus thermophilus sbt1277. | 2007 | 17381740 |
| effect of bovicin hc5 on growth and spore germination of bacillus cereus and bacillus thuringiensis isolated from spoiled mango pulp. | to use bovicin hc5 to inhibit predominant bacteria isolated from spoiled mango pulp. | 2007 | 17381743 |
| proteomic analysis of bacillus cereus growing in liquid soil organic matter. | bacillus cereus is believed to be a soil bacterium, but studied solely in laboratory culture media. the aim of this study was to assess the physiology of b. cereus growing on soil organic matter by a proteomic approach. cells were cultured to mid-exponential phase in soil extracted solubilized organic matter (sesom), which mimics the nutrient composition of soil, and in luria-bertani broth as control. silver staining of the two-dimensional gels revealed 234 proteins spots up-regulated when cells ... | 2007 | 17391366 |
| structural insight into repair of alkylated dna by a new superfamily of dna glycosylases comprising heat-like repeats. | 3-methyladenine dna glycosylases initiate repair of cytotoxic and promutagenic alkylated bases in dna. we demonstrate by comparative modelling that bacillus cereus alkd belongs to a new, fifth, structural superfamily of dna glycosylases with an alpha-alpha superhelix fold comprising six heat-like repeats. the structure reveals a wide, positively charged groove, including a putative base recognition pocket. this groove appears to be suitable for the accommodation of double-stranded dna with a fli ... | 2007 | 17395642 |
| insecticidal bacteria isolated from predatory larvae of the antlion species myrmeleon bore (neuroptera: myrmeleontidae). | various bacterial species were isolated from the crop (digestive organ) of the antlion species myrmeleon bore and tested for their insecticidal activity against caterpillars by injection. sixty-eight isolates from the antlion crop were grouped into twenty-four species based on homologies of 16s rrna gene sequences and biochemical properties. isolated bacillus cereus, bacillus sphaericus, morganella morganii, serratia marcescens and a klebsiella species killed 80% or more cutworms when injected a ... | 2007 | 17399737 |
| differentiation of bacillus anthracis, b. cereus, and b. thuringiensis by using pulsed-field gel electrophoresis. | a pulsed-field gel electrophoresis (pfge) method was developed for discriminating bacillus anthracis from b. cereus and b. thuringiensis. a worldwide collection of 25 b. anthracis isolates showed high-profile homology, and these isolates were unambiguously distinguished from b. cereus and b. thuringiensis isolates by cluster analysis of the whole-genome macrorestriction enzyme digestion patterns generated by noti. | 2007 | 17400781 |
| properties of bacterial corrosion of stainless steel and its inhibition by protamine coating. | we investigated characteristics of the corrosion of stainless steel specimens by bacteria and the effects of using antimicrobial coating on the surface for inhibiting corrosion. bacillus sp. 2-a and staphylococcus sp. 2-1 cells adhered tightly to a stainless steel sus304 specimen, formed a microcolony or biofilm, and had highly corrosive activities. microbially influenced corrosion (mc) was observed under or around adhering cells. however, dead cells were markedly less active than viable cells n ... | 2007 | 17408005 |
| germination of bacillus cereus spores adhered to stainless steel. | adhered spores of bacillus cereus represent a significant part of the surface-derived contamination in processing equipment used in the dairy industry. as germinated spores lose their resistance capacities instantaneously, efficient germination prior to a cleaning in place treatment could aid to the disinfecting effect of such a treatment. therefore, spores of b. cereus atcc 14579 and that of the environmental isolate b. cereus cmcc 3328 were assessed for their germination behaviour when adhered ... | 2007 | 17408793 |
| identification of the sigmab regulon of bacillus cereus and conservation of sigmab-regulated genes in low-gc-content gram-positive bacteria. | the alternative sigma factor sigma(b) has an important role in the acquisition of stress resistance in many gram-positive bacteria, including the food-borne pathogen bacillus cereus. here, we describe the identification of the set of sigma(b)-regulated genes in b. cereus by dna microarray analysis of the transcriptome upon a mild heat shock. twenty-four genes could be identified as being sigma(b) dependent as witnessed by (i) significantly lower expression levels of these genes in mutants with a ... | 2007 | 17416654 |
| bacillus cereus strains fall into two clusters (one closely and one more distantly related) to bacillus anthracis according to amino acid substitutions in small acid-soluble proteins as determined by tandem mass spectrometry. | small acid-soluble proteins (sasps) are located in the core region of bacillus spores and have been previously demonstrated as reliable biomarkers for differentiating bacillus anthracis and bacillus cereus. using ms and ms-ms analysis of sasps further phylogenetic correlations among b. anthracis and b. cereus strains are described here. esi was demonstrated to be a more comprehensive method, allowing for the analysis of intact proteins in both ms and ms-ms mode, thus providing molecular weight ( ... | 2007 | 17197155 |
| the use of flow cytometry to study the germination of bacillus cereus endospores. | at present the study of endospore germination is conducted using microbiological methods which are slow and yield data based on the means of large heterogeneous populations. flow cytometry (fcm) offers the potential to rapidly quantify and identify germination and outgrowth events for large numbers of individual endospores. | 2007 | 17200957 |
| ceramidase enhances phospholipase c-induced hemolysis by pseudomonas aeruginosa. | we previously reported the purification, molecular cloning, and characterization of a neutral ceramidase from pseudomonas aeruginosa strain an17 (okino, n., tani, m., imayama, s., and ito, m. (1998) j. biol. chem. 273, 14368-14373; okino, n., ichinose, s., omori, a., imayama, s., nakamura, t., and ito, m. (1999) j. biol. chem. 274, 36616-36622). interestingly, the gene encoding the enzyme is adjacent to that encoding hemolytic phospholipase c (plch) in the genome of pseudomonas aeruginosa, which ... | 2007 | 17202150 |
| fatal pneumonia among metalworkers due to inhalation exposure to bacillus cereus containing bacillus anthracis toxin genes. | bacillus cereus pneumonia is unusual in nonimmunocompromised hosts. we describe fatal cases in 2 metalworkers and the associated investigation. anthrax toxin genes were identified in b. cereus isolates from both patients using polymerase chain reaction. finding anthrax toxin genes in non-bacillus anthracis isolates has, to our knowledge, only been reported once previously. | 2007 | 17205450 |
| air-liquid interface biofilms of bacillus cereus: formation, sporulation, and dispersion. | biofilm formation by bacillus cereus was assessed using 56 strains of b. cereus, including the two sequenced strains, atcc 14579 and atcc 10987. biofilm production in microtiter plates was found to be strongly dependent on incubation time, temperature, and medium, as well as the strain used, with some strains showing biofilm formation within 24 h and subsequent dispersion within the next 24 h. a selection of strains was used for quantitative analysis of biofilm formation on stainless steel coupo ... | 2007 | 17209076 |
| antimicrobial and cytotoxic knotwood extracts and related pure compounds and their effects on food-associated microorganisms. | knotwood or bark extracts prepared from 30 species of hard and soft wood trees as well as selected pure compounds (lignans, stilbenes and flavonoids) were assayed for their antimicrobial activity against a battery of both gram positive and negative bacteria, yeasts, and filamentous fungi (bacillus cereus, staphylococcus aureus, listeria monocytogenes, lactobacillus plantarum, escherichia coli, salmonella infantis, pseudomonas fluorescens, candida albicans, saccharomyces cerevisiae, aspergillus f ... | 2007 | 17188387 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| virulence of bacillus cereus: a multivariate analysis. | biological activity and presence of dna sequences related to virulence genes were studied in 21 strains of the bacillus cereus group. the activity of spent culture supernatants and the effect of infection by vegetative bacterial cells were assessed on cultured human enterocytes (caco-2 cells). the effect of extracellular factors on the detachment, necrosis and mitochondrial dehydrogenase activity of cultured human enterocytes was studied. hemolytic activity on rabbit red blood cells was also eva ... | 2007 | 17303280 |
| antibacterial effect of some essential oils administered alone or in combination with norfloxacin. | the objective of the present study was that of verifying a possible synergistic antibacterial effect between pelargonium graveolens [lis-balchin, m., deans, s.g., hart, s., 1996. bioactive geranium oils from different commercial sources. j. essential oil res. 8, 281-290.] essential oil (and its main components) and norfloxacin antibiotic. as a first step growth inhibition by some types of essential oils was assessed in five microbial species. the antimicrobial effects of p. graveolens oil, as we ... | 2007 | 17303397 |
| role of zinc content on the catalytic efficiency of b1 metallo beta-lactamases. | metallo beta-lactamases (mbetal) are enzymes naturally evolved by bacterial strains under the evolutionary pressure of beta-lactam antibiotic clinical use. they have a broad substrate spectrum and are resistant to all the clinically useful inhibitors, representing a potential risk of infection if massively disseminated. the mbetal scaffold is designed to accommodate one or two zinc ions able to activate a nucleophilic hydroxide for the hydrolysis of the beta-lactam ring. the role of zinc content ... | 2007 | 17305336 |
| characteristics of bacteriocin-like inhibitory substances from dochi-isolated enterococcus faecium d081821 and d081833. | to characterize bacteriocin-like inhibitory substances (blis) from two dochi-isolated enterococcus faecium. | 2007 | 17309511 |
| characterization of mesophilic bacilli in faeces of feedlot cattle. | to determine the identity and composition of mesophilic bacillus spp. in faeces sampled from feedlot cattle. | 2007 | 17309638 |
| chitin-supplemented foliar application of chitinolytic bacillus cereus reduces severity of botrytis gray mold disease in chickpea under controlled conditions. | to identify and evaluate chitinolytic bacteria for control of botrytis gray mold (bgm), a devastating disease in chickpea. methods and results: two antifungal bacterial isolates, chitinolytic bacillus cereus crs 7 and nonchitinolytic pseudomonas fluorescens crs 31, from the rhizosphere of chickpea, were applied as a prophylactic foliar spray and evaluated for control of bgm. in a controlled environment, the two isolates reduced the severity of bgm on the susceptible cv. jg 62 to 6.0 and 5.6, res ... | 2007 | 17209822 |
| dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase c. | the crystal structure of the w47a/w242a mutant of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus thuringiensis has been solved to 1.8a resolution. the w47a/w242a mutant is an interfacially challenged enzyme, and it has been proposed that one or both tryptophan side chains serve as membrane interfacial anchors (feng, j., wehbi, h., and roberts, m. f. (2002) j. biol. chem. 277, 19867-19875). the crystal structure supports this hypothesis. relative to the crystal structure of ... | 2007 | 17213187 |
| kinetics of plasmid transfer among bacillus cereus group strains within lepidopteran larvae. | the cry toxin encoding plasmid pht73 was transferred from bacillus thuringiensis subspecies kurstaki kt0 to six b. cereus group strains in three lepidopteran (spodoptera exigua, plutella xyllostella and helicoverpa armigera) larvae by conjugation. the conjugation kinetics of the plasmid was precisely studied during the larval infection using a new protocol. the infections were performed with both vegetative and sporulated strains. however, larval death only occurred when infections were made wit ... | 2007 | 17216168 |
| microbial composition in bioaerosols of a high-throughput chicken-slaughtering facility. | the microbial composition of the air in various areas of a high-throughput chicken-slaughtering facility was investigated. over a 4-mo period, 6 processing areas were sampled, and the influence of environmental factors was monitored. the highest counts of microorganisms were recorded in the initial stages of processing, comprising the receiving-killing and defeathering areas, whereas counts decreased toward the evisceration, air-chilling, packaging, and dispatch areas. maximum microbial counts w ... | 2007 | 17179429 |
| predictive modeling of bacillus cereus spores in farm tank milk during grazing and housing periods. | the shelf life of pasteurized dairy products depends partly on the concentration of bacillus cereus spores in raw milk. based on a translation of contamination pathways into chains of unit-operations, 2 simulation models were developed to quantitatively identify factors that have the greatest effect on the spore concentration in milk. in addition, the models can be used to determine the reduction in concentration that could be achieved via measures at the farm level. one model predicts the conce ... | 2007 | 17183096 |
| virulent spores of bacillus anthracis and other bacillus species deposited on solid surfaces have similar sensitivity to chemical decontaminants. | to compare the relative sensitivity of bacillus anthracis and spores of other bacillus spp. deposited on different solid surfaces to inactivation by liquid chemical disinfecting agents. | 2007 | 17184315 |
| the microbiological quality of hot water-washed broccoli florets and cut green beans. | to determine the effect of hot water washing on the microbiological quality of cut broccoli florets and trimmed green beans. | 2007 | 17184318 |
| high level expression of a recombinant phospholipase c from bacillus cereus in bacillus subtilis. | twenty-two bacillus cereus strains were screened for phospholipase c (plc, ec 3.1.4.3) activity using p-nitrophenyl phosphorylcholine as a substrate. two strains (b. cereus sbug 318 and sbug 516) showed high activity at elevated temperatures (>70 degrees c) at acidic ph (ph 3.5-6) and were selected for cloning and functional expression using bacillus subtilis. the genes were amplified from b. cereus dna using primers based on a known plc sequence and cloned into the expression vector pmse3 follo ... | 2007 | 17115205 |
| dna microarray for detection of antibiotic resistance determinants in bacillus anthracis and closely related bacillus cereus. | we developed a multiplex pcr for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in bacillus anthracis and closely related bacillus cereus. enzymatic labelling of pcr products followed by hybridization to oligonucleotide probes on a dna microarray enabled simultaneous detection of resistance genes tetk, tetl, tetm, teto, vana, and vanb and resistance-mediating point mutations in genes gyra, gyrb, parc, and rpob. the presented assay allows ... | 2007 | 17118627 |
| synthesis and antimicrobial activity of 1-(4-aryl-2-thiazolyl)-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives. | several 1-(4-aryl-2-thiazolyl)-3-(2-thienyl)-5-aryl-2-pyrazoline derivatives were synthesized by reacting substituted 3-(2-thienyl)-5-aryl-1-thiocarbamoyl-2-pyrazolines with phenacyl bromides in ethanol. the structures of the synthesized compounds were confirmed by (1)h nmr, (13)c nmr, and eims spectral data. their antimicrobial activities against escherichia coli (nrrl b-3704), staphylococcus aureus (nrll b-767), salmonella typhimurium (nrrl b-4420), bacillus cereus (nrrl b-3711), streptococcus ... | 2007 | 17125888 |
| undetected bacillus pseudo-outbreak after renovation work in a teaching hospital. | a 602-bed capacity hospital underwent complete renovation from 1999 to 2004. in april 2005, the infection control team was informed of the occurrence of three consecutive cases of bacillus cereus bacteremia in a ward for patients with hematologic malignancies. a retrospective analysis of patients with bacillus isolates was initiated. we found more bacillus cereus isolates from blood samples in 2004 compare to the preceding years. swab samples were collected in the particular ward from the surfac ... | 2007 | 17145080 |
| toxicological profile of cereulide, the bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells. | some strains of the endospore-forming bacterium bacillus cereus produce a heat-stable ionophoric peptide, cereulide, of high human toxicity. we assessed cell toxicity of cereulide by measuring the toxicities of crude extracts of cereulide producing and non-producing strains of b. cereus, and of pure cereulide, using cells of human, animal and bacterial origins. hepatic cell lines and boar sperm, with cytotoxicity and sperm motility, respectively, as the end points, were inhibited by 1 nm of cere ... | 2007 | 17156808 |
| a gene encoding alanine racemase is involved in spore germination in bacillus thuringiensis. | alanine racemase is a major component of the exosporium of bacillus cereus spores. a gene homologous to that of alanine racemase (alra) was cloned from bacillus thuringiensis subsp. kurstaki, and rt-pcr showed that alra was transcribed only in the sporulating cells. disruption of alra did not affect the growth and sporulation of b. thuringiensis, but promoted l-alanine-induced spore germination. when the spore germination rate was measured by monitoring dpa release, complementation of the alra d ... | 2007 | 17165028 |
| intractable bacillus cereus bacteremia in a preterm neonate. | although often regarded as a contaminant, bacillus spp. have been implicated in serious systemic infections. the incidence of such infections is low with only a few cases reported in the literature. we describe the clinical course of early-onset bacillus cereus bacteremia in a preterm neonate who was successfully treated with vancomycin. | 2007 | 17166929 |
| ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd) is a convenient stable precursor of bacterial quorum sensing autoinducer ai-2. | ac2-dpd, the bis-(o)-acetylated derivative of 4,5-dihydroxy-2,3-pentanedione (dpd), was prepared both as a racemic mixture and in the optically active form found in naturally occurring dpd. it was shown to exhibit the same ability as dpd to induce bioluminescence in vibrio harveyi and beta-galactosidase activity in salmonella enterica typhimurium, both gram-negative bacteria. likewise, it was also shown to inhibit biofilm formation in gram-positive bacillus cereus. the most likely hypothesis is ... | 2007 | 17169556 |
| the properties of bacillus cereus hemolysin ii pores depend on environmental conditions. | hemolysin ii (hlyii), one of several cytolytic proteins encoded by the opportunistic human pathogen bacillus cereus, is a member of the family of oligomeric beta-barrel pore-forming toxins. this work has studied the pore-forming properties of hlyii using a number of biochemical and biophysical approaches. according to electron microscopy, hlyii protein interacts with liposomes to form ordered heptamer-like macromolecular assemblies with an inner pore diameter of 1.5-2 nm and an outer diameter of ... | 2007 | 17173854 |
| computer aided boar semen motility analysis for cereulide detection in different food matrices. | computer aided semen analysis (casa) study of the boar semen motility has been demonstrated to be an appropriate assay for detection of cereulide (bacillus cereus emetic toxin). application of the boar semen bio-assay to detect cereulide directly in foods requires investigation of potential interference of food components, preservatives and other microbial and chemical food contaminants with the bio-assay. current study provides evidence that none of included staphylococcus aureus enterotoxins a ... | 2007 | 17174428 |
| detection of vibrio cholerae by real-time nucleic acid sequence-based amplification. | a multitarget molecular beacon-based real-time nucleic acid sequence-based amplification (nasba) assay for the specific detection of vibrio cholerae has been developed. the genes encoding the cholera toxin (ctxa), the toxin-coregulated pilus (tcpa; colonization factor), the ctxa toxin regulator (toxr), hemolysin (hlya), and the 60-kda chaperonin product (groel) were selected as target sequences for detection. the beacons for the five different genetic targets were evaluated by serial dilution of ... | 2007 | 17220262 |
| identification of three zwittermicin a biosynthesis-related genes from bacillus thuringiensis subsp. kurstaki strain ybt-1520. | zwittermicin a (zwa) is a novel, broad-spectrum linear aminopolyol antibiotic produced by some bacillus cereus and bacillus thuringiensis. however, only part of its biosynthesis cluster has been identified and characterized from b. cereus uw85. to better understand the biosynthesis cluster of zwa, a bacterial artificial chromosome (bac) library of b. thuringiensis subsp. kurstaki strain ybt-1520, a zwa-producing strain, was constructed. two bac clones, 1f8 and 5e2, were obtained by pcr, which ov ... | 2007 | 17225146 |
| low molecular weight chitosans--preparation with the aid of pronase, characterization and their bactericidal activity towards bacillus cereus and escherichia coli. | the homogeneous low molecular weight chitosans (lmwc) of molecular weight 9.5-8.5 kda, obtained by pronase catalyzed non-specific depolymerization (at ph 3.5, 37 degrees c) of chitosan showed lyses of bacillus cereus and escherichia coli more efficiently (100%) than native chitosan (<50%). ir and (1)h-nmr data showed decrease in the degree of acetylation (14-19%) in lmwc compared to native chitosan ( approximately 26%). minimum inhibitory concentration of lmwc towards 10(6) cfu ml(-1) of b. cere ... | 2007 | 17240531 |
| difference between the spore sizes of bacillus anthracis and other bacillus species. | to determine the size distribution of the spores of bacillus anthracis, and compare its size with other bacillus species grown and sporulated under similar conditions. | 2007 | 17241334 |
| genotypic and phenotypic diversity of lactococcus lactis isolates from batzos, a greek pdo raw goat milk cheese. | the genotypic and phenotypic variability of 40 lactococcus lactis isolates obtained from three cheese-making trials of batzos cheese made one in each, winter, spring and summer was investigated. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. results showed a high degree of heterogeneity among strains. according to pfge data, all strains except one were clustered together (at a similarity level of approximately 50%) with t ... | 2007 | 17241681 |
| hqno-sensitive nadh:quinone oxidoreductase of bacillus cereus kctc 3674. | the enzymatic properties of nadh:quinone oxidoreductase were examined in triton x-100 extracts of bacillus cereus membranes by using the artificial electron acceptors ubiquinone-1 and menadione. membranes were prepared from b. cereus kctc 3674 grown aerobically on a complex medium and oxidized with nadh exclusively, whereas deamino-nadh was determined to be poorly oxidized. the nadh oxidase activity was lost completely by solubilization of the membranes with triton x-100. however, by using the a ... | 2007 | 17244482 |
| asymmetric synthesis of diastereomeric diaminoheptanetetraols. a proposal for the configuration of (+)-zwittermicin a. | [structure: see text] a proposed absolute configuration for the 7 stereocenters in (+)-zwittermicin a is described based on asymmetric synthesis of six diastereomeric 2,6-diamino-1,3,5,7-heptanetetraols corresponding to the c9-c15 segment, pairwise 13c nmr chemical shift difference analysis of the models with the natural product, interpretation of enantiospecificity of the serine loading domain of the zwittermicin a biosynthetic gene cluster, and degradation of the natural product. | 2007 | 17249781 |
| low molecular weight chitosan--preparation with the aid of pepsin, characterization, and its bactericidal activity. | pepsin (ec 3.4.4.1) from porcine stomach mucosa caused depolymerization of a chitosan sample (a copolymer of glucosamine and n-acetylglucosamine linked by beta-1-4-glycosidic bonds). n-terminal sequence and zymogram analyses confirmed dual (proteolytic and chitosanolytic) activities of pepsin. optimum depolymerization occurred at ph 5.0 and 45 degrees c with an activity of 4.98 u. low molecular weight chitosan (lmwc), the major depolymerization product, was obtained in a yield of 75-82%, the deg ... | 2007 | 17256986 |
| the redox regulator fnr is required for fermentative growth and enterotoxin synthesis in bacillus cereus f4430/73. | glucose-grown cells of bacillus cereus respond to anaerobiosis and low extracellular oxidoreduction potentials (orp), notably by enhancing enterotoxin production. this response involves the resde two-component system. we searched the b. cereus genome for other redox response regulators potentially involved in this adaptive process, and we identified one gene encoding a protein predicted to have an amino acid sequence 58% identical (80% similar) to that of the bacillus subtilis fnr redox regulato ... | 2007 | 17259311 |
| diagnostic real-time pcr assays for the detection of emetic bacillus cereus strains in foods and recent food-borne outbreaks. | cereulide-producing bacillus cereus can cause an emetic type of food-borne disease that mimics the symptoms provoked by staphylococcus aureus. based on the recently discovered genetic background for cereulide formation, a novel 5' nuclease (taqman) real-time pcr assay was developed to provide a rapid and sensitive method for the specific detection of emetic b. cereus in food. the taqman assay includes an internal amplification control and primers and a probe designed to target a highly specific ... | 2007 | 17259359 |
| of spore opsonization and passive protection against anthrax. | 2007 | 17259600 | |
| method of measuring bacillus anthracis spores in the presence of copious amounts of bacillus thuringiensis and bacillus cereus. | a sensitive and reliable method for the detection of bacillus anthracis (ba; sterne strain 7702) spores in presence of large amounts of bacillus thuringiensis (bt) and bacillus cereus (bc) is presented based on a novel pzt-anchored piezoelectric excited millimeter-sized cantilever (papemc) sensor with a sensing area of 1.5 mm2. antibody (anti-ba) specific to ba spores was immobilized on the sensing area and exposed to various samples of ba, bt, and bc containing the same concentration of ba at 3 ... | 2007 | 17263347 |
| role of bacterial and host factors in infectious endophthalmitis. | endophthalmitis is a frequent blinding complication of globe-penetrating injury and ocular surgery. the outcome of this intraocular infection depends both on the organism involved and management of the ensuing inflammation. the role of various toxins and bacterial factors in the pathogenesis of this infection is beginning to be delineated, but appears to be organism specific. because of the immune-privileged environment of the eye, principles important in the resolution of infection at extraocul ... | 2007 | 17264502 |
| titanium dioxide/uv photocatalytic disinfection in fresh carrots. | increased occurrences of fresh produce-related outbreaks of foodborne illness have focused attention on effective washing processes for fruits and vegetables. a titanium dioxide (tio2) photocatalytic reaction under uv radiation provides a high rate of disinfection. the photo-killing effects of tio2 on bacteria in liquid cultures under experimental conditions have been widely studied. however, the disinfection effects of the tio2 photocatalytic reaction on fresh vegetables during a washing proces ... | 2007 | 17265866 |
| use of high-affinity cell wall-binding domains of bacteriophage endolysins for immobilization and separation of bacterial cells. | immobilization and magnetic separation for specific enrichment of microbial cells, such as the pathogen listeria monocytogenes, depends on the availability of suitable affinity molecules. we report here a novel concept for the immobilization and separation of bacterial cells by replacing antibodies with cell wall-binding domains (cbds) of bacteriophage-encoded peptidoglycan hydrolases (endolysins). these polypeptide modules very specifically recognize and bind to ligands on the gram-positive cel ... | 2007 | 17277212 |
| structural studies of thymidine kinases from bacillus anthracis and bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding. | thymidine kinase (tk) is the key enzyme in salvaging thymidine to produce thymidine monophosphate. owing to its ability to phosphorylate nucleoside analogue prodrugs, tk has gained attention as a rate-limiting drug activator. we describe the structures of two bacterial tks, one from the pathogen bacillus anthracis in complex with the substrate dt, and the second from the food-poison-associated bacillus cereus in complex with the feedback inhibitor dttp. interestingly, in contrast with previous s ... | 2007 | 17288553 |
| food poisoning potential of bacillus cereus strains from norwegian dairies. | characteristics concerning diarrhoeal potential were investigated in b. cereus dairy strains. the thirty-nine strains, isolated from whipping cream, were tested for cytotoxicity after culturing at human body temperature as well as 25 degrees c and 32 degrees c. at 37 degrees c, none of the strains were highly cytotoxic. this observation suggests that those strains should be considered to pose a minor risk with regard to diarrhoeal food poisoning. however, some strains were moderately or highly c ... | 2007 | 17292990 |
| degradation of n-acyl-l-homoserine lactones by bacillus cereus in culture media and pork extract. | degradation of the quorum-sensing signal molecule n-acyl-l-homoserine lactone (ahl) in cocultures was verified with bacillus cereus and yersinia enterocolitica in culture medium and in pork extract. results showed evidence of microbial interaction when the ahl-degrading bacterium and ahl-producing bacterium were cocultured in a food-simulating condition. | 2007 | 17293532 |
| changes in numbers and kinds of bacteria during a chickpea submerged fermentation used as a leavening agent for bread production. | the microflora developed during a submerged fermentation of coarsely ground chickpea (cicer arietinum l.) in water (primary starter) and during raising a dough from wheat flour (adapted starter) was studied. in the fermenting liquid, only populations of bacillus and clostridium developed. bacilli increased their loads significantly (p<0.05) during fermentation for 8-12 h and then remained constant. clostridia developed (p<0.05) subsequently to levels of 10(7) cfu/ml at 18 h, when the ph of the f ... | 2007 | 17300848 |
| group ii intron in bacillus cereus has an unusual 3' extension and splices 56 nucleotides downstream of the predicted site. | all group ii introns known to date fold into six functional domains. however, we recently identified an intron in bacillus cereus atcc 10987, b.c.i4, that splices 56 nt downstream of the expected 3' splice site in vivo (tourasse et al. 2005, j. bacteriol., 187, 5437-5451). in this study, we confirmed by ribonuclease protection assay that the 56-bp segment is part of the intron rna molecule, and computational prediction suggests that it might form a stable stem-loop structure downstream of domain ... | 2007 | 17301069 |
| antibacterial effect of five zingiberaceae essential oils. | essential oil obtained by hydrodistillation and two different solvent extractions (petroleum ether and ethanol) from five zingiberaceae species: ginger (zingiber officinale roscoe.), galanga (alpinia galanga sw.), turmeric (curcuma longa l.), kaempferia (boesenbergia pandurata holtt.) and bastard cardamom (amomum xanthioides wall.) was characterized. volatile components of all extracts were analyzed by gas chromatography-mass spectrometry (gc-ms). the major components of ginger, turmeric, galang ... | 2007 | 17960105 |
| [effects of rhamnolipid on the biodegradation of n-hexadecane by microorganism and the cell surface hydrophobicity]. | the experiments were made in laboratory to analyze the effects of rhamnolipid on the biodegradation of n-hexadecane and the cell surface hydrophobicity using the microorganism ( bacillus cereus dq01 and bacillus sp. dq02). the results show that the optimal concentrations range of rhamnolipid were 0.4 mmol/l and 0.2 mmol/l, respectively, which can obviously enhance bath(bacterial adherence to hydrocarbon). the degradation of n-hexadecane by bacillus cereus dq01 and bacillus sp. dq02 were increase ... | 2007 | 17990568 |
| metabolic capacity of bacillus cereus strains atcc 14579 and atcc 10987 interlinked with comparative genomics. | bacillus cereus is an important food-borne pathogen and spoilage organism. in this study, numerous phenotypes and the genomes of b. cereus strains atcc 14579 and atcc 10987 were analysed to compare their metabolic capacity and stress resistance potential. the growth performance of the two strains was assessed for nearly 2000 phenotypes, including use of nutrient sources, performance in acid and basic environments, osmo-tolerance and antibiotic resistance. several food-relevant phenotypic differe ... | 2007 | 17991024 |
| effects of low electric current (lec) treatment on pure bacterial cultures. | this research focused on the effects of low electric current (lec) on the cell viability and metabolic activity of escherichia coli and bacillus cereus. | 2007 | 17953548 |
| isolation and selection of bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. | to isolate, select and evaluate bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. | 2007 | 17953558 |
| effects of porcine bile on survival of bacillus cereus vegetative cells and haemolysin bl enterotoxin production in reconstituted human small intestine media. | to determine the effects of porcine bile (pb) on bacillus cereus vegetative cells and haemolysin bl (hbl) enterotoxin production in reconstituted small intestine media (im). | 2007 | 17953568 |
| characterization, n-terminal sequencing and classification of cerein mrx1, a novel bacteriocin purified from a newly isolated bacterium: bacillus cereus mrx1. | to purify and characterize the bacteriocin produced by strain mrx1. | 2007 | 17953573 |
| microwave-accelerated metal-enhanced fluorescence: application to detection of genomic and exosporium anthrax dna in <30 seconds. | we describe the ultra-fast and sensitive detection of the gene encoding the protective antigen of bacillus anthracis the causative agent of anthrax. our approach employs a highly novel platform technology, microwave-accelerated metal-enhanced fluorescence (mamef), which combines the use of metal-enhanced fluorescence to enhance assay sensitivity and focused microwave heating to spatially and kinetically accelerate dna hybridization. genomic and exosporium target dna of bacillus anthracis spores ... | 2007 | 17955147 |
| pseudochromhidrosis: blue discolouration of the head and neck. | a 57-year-old man is presented with blue pseudochromhidrosis affecting the face and neck following combination treatment with lansoprazole, a proton pump inhibitor, and ranitidine, a type two histamine receptor antagonist. the diagnosis was made on the basis of clinico-histological features and growth of malassezia furfur, and bacillus species, not bacillus cereus, in the absence of lipofuscin. the pseudochromhidrosis resolved on stopping both medications and did not recur on restarting only the ... | 2007 | 17956484 |
| culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection with aerobic culture. | bacterial contamination of platelet (plt) products occurs at low concentrations requiring a period of incubation for growth to minimize sampling error. culture-based detection methods also need sufficient incubation time; together these periods may limit the useful life of plts. this study characterizes the impact of sampling and detection times with two commercially available bacteria detection products. | 2007 | 17958533 |
| detection, isolation, and identification of cadmium-resistant bacteria based on pcr-dgge. | this study focused on the screening of cadmium-resistant bacterial strains from pb-zn tailing. we investigated the diversity of microbial community inhabiting dong-san-cha pb-zn tailing in beijing, china, by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) of 16s rrna gene of bacterial strain, and found two dominant strains in the dgge profile. using special culture media, we isolated two strong cadmium-resistant bacterial strains. on the basis of morphological, physiolog ... | 2007 | 17966518 |