Publications

population research and training in u.s. schools of public health.		2015	4719535
clotting factor concentrates.		2015	5008488
[meningomyeloceles and sacral agenesis. urologic problesm in infants].		2015	4619894
[synoptic demonstration of antiepidemic measures].		2015	4641580
[comparative study of the preventive effect of different dentifrices by toothbrushing].		2015	4511685
doctor thomas hodgkin and sir moses montefiore.		2015	4900117
[the influence of hydrologic conditions of the volgograd water reservoir on the breeding of blood-sucking mosquitoes in the floodlands of the volga].		2015	4388225
evidence for biosynthetical equivalence of the epimeric isoleucine residues in angolide.		2015	4337566
transfer of metabolites across the nuclear membrane. a microfluorometric study.		2015	4327736
[acute adaline poisoning].		2015	4384384
elevated cpxr~p levels repress the ysc-yop type iii secretion system of yersinia pseudotuberculosis.	one way that gram-negative bacteria respond to extracytoplasmic stress is through the cpxa-cpxr system. an activated cpxa sensor kinase phosphorylates the cpxr response regulator to instigate positive auto-amplification of cpx pathway activation, as well as synthesis of various bacterial survival factors. in the absence of cpxa, human enteropathogenic yersinia pseudotuberculosis accumulates high cpxr~p levels aided by the action of low molecular weight phosphodonors such as acetyl~p. critically, ...	2015	22842077
intestinal alkaline phosphatase deficiency leads to lipopolysaccharide desensitization and faster weight gain.	animals develop in the presence of complex microbial communities, and early host responses to these microbes can influence key aspects of development, such as maturation of the immune system, in ways that impact adult physiology. we previously showed that the zebrafish intestinal alkaline phosphatase (alpi) gene alpi.1 was induced by gram-negative bacterium-derived lipopolysaccharide (lps), a process dependent on myeloid differentiation primary response gene 88 (myd88), and functioned to detoxif ...	2015	25348635
[study of yersinia pseudotuberculosis surface antigen epitopes using monoclonal antibodies].	a study of the influence of exogenous factors on the immunochemical activity of the bacterium yersinia pseudotuberculosis and lipopolysaccharide preparations isolated from bacteria was performed using monoclonal antibodies. it was shown that the hybridomas that were obtained in this work produce antibodies against different and, most likely, species-specific epitopes associated with lipopolysaccharide o side chains. the antibody concentrations produced increased with a decrease in the temperatur ...	2015	25272740
structure and genetic basis of yersinia similis serotype o:9 o-specific polysaccharide.	the o-polysaccharide (ops, o-ag) cap of lps is a major virulence factor of yersinia species and also serves as a receptor for the binding of lytic bacteriophage φr1-37. currently, the ops-based serotyping scheme for the yersinia pseudotuberculosis complex includes 21 known o-serotypes that follow three distinct lineages: y. pseudotuberculosis sensu stricto, y. similis and the korean group of strains. elucidation of the y. pseudotuberculosis complex ops structures and characterization of the ops ...	2015	24361587
aberrant forms of yersinia pseudotuberculosis as spheroplasts and filaments in yersiniosis in squirrel monkeys.	this report describes atypical cases of yersiniosis in squirrel monkeys in which aberrant forms of yersinia pseudotuberculosis were seen. there were 2 outbreaks due to yersiniosis in squirrel monkeys in japan. the monkeys had systemic necrotizing and hemorrhagic lesions with gram-negative rod-shaped bacilli and microthromboembolism in the kidneys. some lesions contained filaments, globular bodies, and other pleomorphic forms of bacteria. all forms were usually seen in the same lesions, and those ...	2015	24793827
characterisation of yersinia pseudotuberculosis isolated from animals with yersiniosis during 1996-2013 indicates the presence of pathogenic and far eastern strains in italy.	yersinia pseudotuberculosis is a pathogen that infects both animals and humans worldwide. the epidemiology of infection caused by y. pseudotuberculosis is poorly understood; however, its outbreaks have been traced back to a probable source in wildlife. this study aimed to characterise y. pseudotuberculosis isolates collected from animals with yersiniosis. this study included 90 isolates of y. pseudotuberculosis collected from different animals with yersiniosis between 1996 and 2013 in italy. the ...	2015	26344040
yscu/flhb of yersinia pseudotuberculosis harbors a c-terminal type iii secretion signal.	all type iii secretion systems (t3ss) harbor a member of the yscu/flhb family of proteins that is characterized by an auto-proteolytic process that occurs at a conserved cytoplasmic npth motif. we have previously demonstrated that yscucc, the c-terminal peptide generated by auto-proteolysis of yersinia pseudotuberculosis yscu, is secreted by the t3ss when bacteria are grown in ca(2+)-depleted medium at 37 °c. here, we investigated the secretion of this early t3s-substrate and showed that yscucc ...	2015	26338709
pathogenic yersinia promotes its survival by creating an acidic fluid-accessible compartment on the macrophage surface.	microbial pathogens and host immune cells each initiate events following their interaction in an attempt to drive the outcome to their respective advantage. here we show that the bacterial pathogen yersinia pseudotuberculosis sustains itself on the surface of a macrophage by forming acidic fluid-accessible compartments that are partially bounded by the host cell plasma membrane. these yersinia-containing acidic compartments (yacs) are bereft of the early endosomal marker eea1 and the lysosomal a ...	2015	26275291
[characteristics of formation, inhibition and destruction of yersinia pseudotuberculosis biofilms forming on abiotic surfaces].	detection of conditions of yersinia pseudotuberculosis biofilm formation, their quantitative testing.	2015	26259274
the dual transcriptional regulator rovm regulates the expression of ar3- and t6ss4-dependent acid survival systems in response to nutritional status in yersinia pseudotuberculosis.	coordinated regulation of various acid survival systems in response to environmental stimuli is crucial for the adaptation of enteropathogenic bacteria to acidic environments such as the stomach. in this study, we demonstrated that the rovm protein, a central regulator of the csrabc-rovm-rova cascade, conversely regulates the expression of two acid survival systems in yersinia pseudotuberculosis by acting as a dual transcriptional regulator. rovm activated the expression of t6ss4, which is essen ...	2015	26234561
meat processing plant microbiome and contamination patterns of cold-tolerant bacteria causing food safety and spoilage risks in the manufacture of vacuum-packaged cooked sausages.	refrigerated food processing facilities are specific man-made niches likely to harbor cold-tolerant bacteria. to characterize this type of microbiota and study the link between processing plant and product microbiomes, we followed and compared microbiota associated with the raw materials and processing stages of a vacuum-packaged, cooked sausage product affected by a prolonged quality fluctuation with occasional spoilage manifestations during shelf life. a total of 195 samples were subjected to ...	2015	26231646
the crystal structure of heme acquisition system a from yersinia pseudotuberculosis (hasaypt): roles of the axial ligand tyr75 and two distal arginines in heme binding.	some gram-negative pathogens utilize an extracellular heme-binding protein called hemophore to satisfy their needs for iron, a metal element essential for most living things. we report here crystal structures of heme acquisition system a from yersinia pseudotuberculosis (hasaypt) and its y75a mutant. the wild-type hasaypt structure revealed that the heme iron is coordinated with tyr75 and a water molecule. the heme-bound water molecule makes extensive hydrogen bond network that includes arg40 an ...	2015	26210321
[immunochemical activity of yersinia pseudotuberculosis b-antigen].	a hybridoma panel producing monoclonal antibodies to immunochemically non-identical antigenic epitopes of the protein nature located in outer membrane of yersinia pseudotuberculosis was obtained. it was revealed that the previously identified b-antigen protecting laboratory animals from experimental plague was detected using both monoclonal antibodies against mentioned protein determinants and the determinants of lipopolysaccharide o-side chains. the b-antigen is a component of the y. pseudotube ...	2015	26182665
yersiniosis due to infection by yersinia pseudotuberculosis 4b in captive meerkats (suricata suricatta) in japan.	two meerkats (suricata suricatta) housed in the same zoological garden in japan died due to yersinia pseudotuberculosis serotype 4b infection. gross and microscopic lesions included necrotizing enteritis and enlargement of the spleen and liver with multifocal necrosis. inflammatory cells, primarily neutrophils, and nuclear debris were associated with clusters of gram-negative bacilli. additionally, there were aberrant organism forms that were larger than bacilli and appeared as basophilic globul ...	2015	26179097
immunochromatographic system for diagnostics of pseudotuberculosis.	we developed a model immunochromatographic test-system for serological express-diagnostics of pseudotuberculosis. nitrocellulose membrane sensitized with species-specific antigen, yersinia pseudotuberculosis thermostable toxin, was used as the immunosorbent. detection of antibodies to the pathogen was performed using functionalized carbon nanoparticles. system performance was verified in testing of the reference pseudotuberculosis serum. tests with salmonella, escherichia, and enteral yersinia d ...	2015	25894787
method for fluorescent marker swapping and its application in steinernema nematode colonization studies.	an allelic exchange vector was constructed to replace gfp by mcherry in bacteria previously tagged with mini-tn5 derivatives. the method was successfully applied to a gfp-labeled yersinia pseudotuberculosis strain and the re-engineered bacterium was used to study the colonization of steinernema nematodes hosting their xenorhabdus symbiont using dual-color confocal microscopy.	2015	25835465
spontaneous ascitic fluid infection and bacteremia due to yersinia pseudotuberculosis in a liver transplant patient.	we report herein a case of bacteremic ascitic fluid infection in a liver transplant patient caused by a strain of yersinia pseudotuberculosis serogroup i that lost the yersiniabactin core. the patient's outcome was favorable after a combined therapy with a third-generation cephalosporin and gentamicin.	2015	25835101
disseminated yersinia pseudotuberculosis infection in a paca (cuniculus paca).	a 2-yr-old paca (cuniculus paca) was presented for necropsy with a history of sudden death. gross examination revealed multifocal, transmural, well-demarcated, white, soft nodules scattered along the length of the small intestine. the liver also had similar nodules associated with the capsular and cut surface. histologic evaluation of several organs, including the intestine, liver, lung, kidney, adrenal gland, and lymph nodes, was consistent with disseminated yersiniosis. in addition, aerobic ba ...	2015	25831586
transcriptomic profiling of yersinia pseudotuberculosis reveals reprogramming of the crp regulon by temperature and uncovers crp as a master regulator of small rnas.	one hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. in order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative rna-seq-based transcriptomic profiling of the enteric pathogen y. pseudotuberculosis under environmental and infection-relevant conditions. we identified 1151 individual transcription start sites, multiple riboswitch-like rna elements, and a global set of ant ...	2015	25816203
superantigenic yersinia pseudotuberculosis induces the expression of granzymes and perforin by cd4+ t cells.	bacterial superantigens (sags) are immunostimulatory toxins that induce acute diseases mainly through the massive release of inflammatory cytokines. yersinia pseudotuberculosis is the only gram-negative bacterium known to produce a sag (y. pseudotuberculosis-derived mitogen [ypm]). this sag binds major histocompatibility complex class ii molecules on antigen-presenting cells and t cell receptors (tcr) bearing the variable region vβ3, vβ9, vβ13.1, or vβ13.2 (in humans) and vβ7 or vβ8 (in mice). w ...	2015	25754199
proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident cd8⁺ t cells responding to infection.	we report that oral infection with yersinia pseudotuberculosis results in the development of two distinct populations of pathogen-specific cd8(+) tissue-resident memory t cells (trm cells) in the lamina propria. cd103(-) t cells did not require transforming growth factor-β (tgf-β) signaling but were true resident memory cells. unlike cd103(+)cd8(+) t cells, which were tgf-β dependent and were scattered in the tissue, cd103(-)cd8(+) t cells clustered with cd4(+) t cells and cx3cr1(+) macrophages ...	2015	25706747
inflammasome activation in response to the yersinia type iii secretion system requires hyperinjection of translocon proteins yopb and yopd.	type iii secretion systems (t3ss) translocate effector proteins into target cells in order to disrupt or modulate host cell signaling pathways and establish replicative niches. however, recognition of t3ss activity by cytosolic pattern recognition receptors (prrs) of the nucleotide-binding domain leucine rich repeat (nlr) family, either through detection of translocated products or membrane disruption, induces assembly of multiprotein complexes known as inflammasomes. macrophages infected with y ...	2015	25691590
identification of mammalian proteins that collaborate with type iii secretion system function: involvement of a chemokine receptor in supporting translocon activity.	the type iii secretion system (t3ss) is a highly conserved protein delivery system found in multiple gram-negative pathogens, including yersinia pseudotuberculosis. most studies of yersinia species type iii intoxication of host cells have focused on the bacterial determinants that promote assembly and function of the secretion system. in this study, we performed a pooled rna interference (rnai) screen to identify mammalian host proteins required for the cytotoxic effects associated with the yers ...	2015	25691588
yersinia pseudotuberculosis yopd mutants that genetically separate effector protein translocation from host membrane disruption.	the yersinia type iii secretion system (t3ss) translocates yop effector proteins into host cells to manipulate immune defenses such as phagocytosis and reactive oxygen species (ros) production. the t3ss translocator proteins yopb and yopd form pores in host membranes, facilitating yop translocation. while the yopd amino and carboxy termini participate in pore formation, the role of the yopd central region between amino acids 150-227 remains unknown. we assessed the contribution of this region by ...	2015	25684661
long-term persistence of yersinia pseudotuberculosis in entomopathogenic nematodes.	entomopathogenic nematodes (epns) are small worms whose ecological behaviour consists to invade, kill insects and feed on their cadavers thanks to a species-specific symbiotic bacterium belonging to any of the genera xenorhabdus or photorhabdus hosted in the gastro-intestinal tract of epns. the symbiont provides a number of biological functions that are essential for its epn host including the production of entomotoxins, of enzymes able to degrade the insect constitutive macromolecules and of an ...	2015	25635766
strains of yersinia wautersii should continue to be classified as the 'korean group' of the yersinia pseudotuberculosis complex and not as a separate species.		2015	25505347
community behavior and spatial regulation within a bacterial microcolony in deep tissue sites serves to protect against host attack.	bacterial pathogens express virulence-specific transcriptional programs that allow tissue colonization. although phenotypic variation has been noted in the context of antibiotic exposure, no direct evidence exists for heterogeneity in virulence-specific transcriptional programs within tissues. in a mouse model of yersinia pseudotuberculosis infection, we show that at least three subpopulations of bacteria develop within a single tissue site in response to distinct host signals. bacteria growing ...	2015	25500192
a single bacterial immune evasion strategy dismantles both myd88 and trif signaling pathways downstream of tlr4.	during bacterial infections, toll-like receptor 4 (tlr4) signals through the myd88- and trif-dependent pathways to promote pro-inflammatory and interferon (ifn) responses, respectively. bacteria can inhibit the myd88 pathway, but if the trif pathway is also targeted is unclear. we demonstrate that, in addition to myd88, yersinia pseudotuberculosis inhibits trif signaling through the type iii secretion system effector yopj. suppression of trif signaling occurs during dendritic cell (dc) and macro ...	2015	26651944
yersinia pseudotuberculosis aortitis in a patient with diverticulosis and polycystic kidney disease.	an 81-year-old gentleman with chronic kidney disease presented with pyrexia and a new systolic cardiac murmur. investigations revealed infective aortitis of a pre-existing aortic aneurysm graft repair. peripheral blood cultures were positive for yersinia pseudotuberculosis and the patient was successfully treated with an extended course of antibiotics. abdominal imaging also revealed progressive bilateral polycystic kidney disease with associated diverticular disease, which was postulated as the ...	2015	26634143
yersinia pseudotuberculosis infection in kawasaki disease and its clinical characteristics.	the etiology of kawasaki disease (kd) is unknown. reportedly, there is an association between kd and yersinia pseudotuberculosis (ypt). steroid therapy for kd patients with high risk of cardiac sequelae (cs) has been reported; however, the number of reports is limited.	2015	26561332
outbreak of yersinia pseudotuberculosis o:1 infection associated with raw milk consumption, finland, spring 2014.	in march 2014, a yersinia pseudotuberculosis (yp) outbreak was detected by a municipal authority in southern finland. we conducted epidemiological, microbiological and traceback investigations to identify the source. we defined a case as a person with yp infection notified to the national infectious disease registry between february and april 2014, or their household member, with abdominal pain and fever≥38 °c or erythema nodosum. healthy household members were used as household-matched controls ...	2015	26537540
bacteriomimetic invasin-functionalized nanocarriers for intracellular delivery.	intracellular bacteria invade mammalian cells to establish an infectious niche. the current work models adhesion and subsequent internalization strategy of pathogenic bacteria into mammalian cells to design a bacteriomimetic bioinvasive delivery system. we report on the surface functionalization of liposomes with a c-terminal fragment of invasin (inva497), an invasion factor in the outer membrane of yersinia pseudotuberculosis. inva497-functionalized liposomes adhere to mammalian epithelial hep- ...	2015	26522071
oral administration of a recombinant attenuated yersinia pseudotuberculosis strain elicits protective immunity against plague.	a yersinia pseudotuberculosis pb1+ (yptb pb1+) mutant strain combined with chromosome insertion of the caf1r-caf1a-caf1m-caf1 operon and deletions of yopj and yopk, χ10068 [pyv-ω2 (δyopj315 δyopk108) δlacz044::caf1r-caf1m-caf1a-caf1] was constructed. results indicated that gene insertion and deletion did not affect the growth rate of χ10068 compared to wild-type yptb cultured at 26 °c. in addition, the f1 antigen in χ10068 was synthesized and secreted on the surface of bacteria at 37 °c (mammali ...	2015	26514425
ccr2+ inflammatory dendritic cells and translocation of antigen by type iii secretion are required for the exceptionally large cd8+ t cell response to the protective yope69-77 epitope during yersinia infection.	during yersinia pseudotuberculosis infection of c57bl/6 mice, an exceptionally large cd8+ t cell response to a protective epitope in the type iii secretion system effector yope is produced. at the peak of the response, up to 50% of splenic cd8+ t cells recognize the epitope yope69-77. the features of the interaction between pathogen and host that result in this large cd8+ t cell response are unknown. here, we used y. pseudotuberculosis strains defective for production, secretion and/or transloca ...	2015	26468944
the infectious cause of the chronic effect.	while acute infections cause short-term tissue damage, their long-term impact remains unknown. in a recent publication in cell, morais da fonseca et al. (2015) demonstrate disruption of mesenteric lymph nodes and associated lymphatics after yersinia pseudotuberculosis infection and clearance. this leads to chronic inflammation and an inability to initiate subsequent intestinal immune responses.	2015	26468738
[yersinia pseudotuberculosis septicemia. case report].		2015	26437760
yersinia pseudotuberculosis, serogroup o:1a, infection in two amazon parrots (amazona aestiva and amazona oratrix) with hepatic hemosiderosis.	necropsies were conducted on a female blue-fronted amazon (amazona aestiva) and a female yellow-headed amazon (amazona oratrix) that died after depression, ruffled feathers, diarrhea, and biliverdin in the urine. gross and microscopic examinations revealed multifocal necrosis in the liver, spleen, lungs, kidneys, intestines, and heart caused by acute bacteremia. yersinia pseudotuberculosis, serogroup o:1a, was isolated by culturing from the visceral lesions in the liver, intestines, and spleen. ...	2015	26352966
opposite effects of lysophosphatidylethanolamines on conformation of ompf-like porin from yersinia pseudotuberculosis.	lysophosphatidyletnolamine (lpe) is one of enigmatic lipids of bacteria. it is generated from major membrane lipid - phosphatidylethanolamine at severe changes of the bacterial growth conditions. accumulation of this phospholipid in cells of gram-negative enterobacterium yersinia pseudotuberculosis results in the enhanced thermostability of ompf-like porin (yompf) from the same bacteria. the respective integral conformational rearrangements may disturb the channel permeability of protein under s ...	2015	26349609
pseudomonas fluorescens alters the intestinal barrier function by modulating il-1β expression through hematopoietic nod2 signaling.	ileal crohn's disease is related to nod2 mutations and to a gut barrier dysfunction. pseudomonas fluorescens has also been associated with ileal crohn's disease. the aim of this study was to determine the impact of p. fluorescens on the paracellular permeability in ileum and peyer's patches.	2015	25659087
[sanitary significance of soil sea coasts].	there was investigated the dynamics of growth of listeria monocytogenes and yersinia pseudotuberculosis in soils of sea coast (mid-flight and maritime soils). these bacteria were shown to reproduced well in all researched soils, preferring nevertheless maritime soils. the content of the humus was determined to be the one of the limiting factors restricting the multiplication of pathogenic bacteria in studied soils. abiotic characteristic of soils of sea coast were established to render the direc ...	2015	26625609
[the metabolism of the innate immunity cells in bacterial infections].	metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ...	2015	25762604
[effect of volatile metabolites from germinating seeds on the reproduction of the bacteria listeria monocytogenes and yersinia pseudotuberculosis].	the biological activity of volatile metabolites of germinating seeds of cabbage (brassica oleacia), carrot (daukus carota), salad (lactuca sativa), and corn (zea mays l.) against listeria monocytogenes and yersinia pseudotuberculosis was studied. it was shown that volatile metabolites are transfer factors and can be the sole carbon and energy source for these bacteria. methanol is the main substance affecting their growth and reproduction.	2015	22834302
comparative genomic hybridization analysis of yersinia enterocolitica and yersinia pseudotuberculosis identifies genetic traits to elucidate their different ecologies.	enteropathogenic yersinia enterocolitica and yersinia pseudotuberculosis are both etiological agents for intestinal infection known as yersiniosis, but their epidemiology and ecology bear many differences. swine are the only known reservoir for y. enterocolitica 4/o:3 strains, which are the most common cause of human disease, while y. pseudotuberculosis has been isolated from a variety of sources, including vegetables and wild animals. infections caused by y. enterocolitica mainly originate from ...	2015	26605338
homology analysis and cross-immunogenicity of ompa from pathogenic yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis.	the outer membrane protein a (ompa) is one of the intra-species conserved proteins with immunogenicity widely found in the family of enterobacteriaceae. here we first confirmed ompa is conserved in the three pathogenic yersinia: yersinia pestis, yersinia pseudotuberculosis and pathogenic yersinia enterocolitica, with high homology at the nucleotide level and at the amino acid sequence level. the identity of ompa sequences for 262 y. pestis strains, 134 y. pseudotuberculosis strains and 219 patho ...	2015	26435220
molecular docking based screening of neem-derived compounds with the ns1 protein of influenza virus.	different strains of influenza virus are affecting a large number of people worldwide to combat with influenza virus destruction, numerous synthetic antiviral medicines are available for influenza virus in the market. but still there was a need for the development of drug which will target all the strains of influenza virus. for this purpose conserved residues within the influenza virus ns1 protein have been found by aligning all the available sequences of existing strains from the national cent ...	2015	26339153
genotypic and phenotypic virulence characteristics and antimicrobial resistance of yersinia spp. isolated from meat and milk products.	a total of 300 food samples including 180 milk and 120 meat products have been examined for the presence of yersinia spp. using the iso 10273 and the cold enrichment method. the overall prevalence of yersinia spp. was 84 (28%). yersinia enterocolitica was isolated from 18 (6%) of the 300 samples. the other yersinia species were detected in the samples yersinia rohdei 15 (5%), yersinia intermedia 14 (4.7%), yersinia pseudotuberculosis 12 (4%), yersinia ruckeri 12 (4%), yersinia mollaretii 5 (1.7% ...	2015	25969137
yersinia adhesin a (yada)--beauty & beast.	the trimeric autotransporter adhesin yersinia adhesin a is the prototype of the type vc secretion systems. it is expressed by enteropathogenic yersinia enterocolitica and yersinia pseudotuberculosis strains, but not by yersinia pestis. a characteristic trait of yada is its modular composition and trimeric nature. yada consists of an n-terminal passenger domain which is exposed on the bacterial cell surface. the translocation of this passenger onto the surface is facilitated by a c-terminal β-bar ...	2015	25604505
yersiniabase: a genomic resource and analysis platform for comparative analysis of yersinia.	yersinia is a gram-negative bacteria that includes serious pathogens such as the yersinia pestis, which causes plague, yersinia pseudotuberculosis, yersinia enterocolitica. the remaining species are generally considered non-pathogenic to humans, although there is evidence that at least some of these species can cause occasional infections using distinct mechanisms from the more pathogenic species. with the advances in sequencing technologies, many genomes of yersinia have been sequenced. however ...	2015	25591325
isolation and characterization of yersinia-specific bacteriophages from pig stools in finland.	bacteriophages infect bacteria, and they are present everywhere in the world including the intestinal tracts of animals. yersiniosis is a common foodborne infection caused by yersinia enterocolitica and yersinia pseudotuberculosis. as these bacteria are frequently isolated from pigs, we wanted to know whether yersinia-specific bacteriophages are also present in the pig stools and, if so, whether there is a positive or negative association between the prevalence of the yersinia phages and the pat ...	2015	25495090
complete protection against pneumonic and bubonic plague after a single oral vaccination.	no efficient vaccine against plague is currently available. we previously showed that a genetically attenuated yersinia pseudotuberculosis producing the yersinia pestis f1 antigen was an efficient live oral vaccine against pneumonic plague. this candidate vaccine however failed to confer full protection against bubonic plague and did not produce f1 stably.	2015	26473734
yersinia pestis and yersinia pseudotuberculosis infection: a regulatory rna perspective.	yersinia pestis, responsible for causing fulminant plague, has evolved clonally from the enteric pathogen, y. pseudotuberculosis, which in contrast, causes a relatively benign enteric illness. an ~97% nucleotide identity over 75% of their shared protein coding genes is maintained between these two pathogens, leaving much conjecture regarding the molecular determinants responsible for producing these vastly different disease etiologies, host preferences and transmission routes. one idea is that c ...	2015	26441890
redefining the differences in gene content between yersinia pestis and yersinia pseudotuberculosis using large-scale comparative genomics.	yersinia pestis, the causative agent of plague, is best known for historical pandemics, but still actively causes disease in many parts of the world. y. pestis is a recently derived clone of the pathogenic species yersinia pseudotuberculosis, but is more associated with human infection. numerous studies have documented genomic changes since the two species differentiated, although all of these studies used a relatively small sample set for defining these differences. in this study, we compared t ...	2015	28348813
[establishment and evaluation of identification method for yersinia pestis and yersinia pseudotuberculosis].	to establish a gene identification method of yersinia pestis and yersinia pseudotuberculosis for plague surveillance.	2015	26080641
generation of a crispr database for yersinia pseudotuberculosis complex and role of crispr-based immunity in conjugation.	the clustered regularly interspaced short palindromic repeat - crispr-associated genes (crispr-cas) system is used by bacteria and archaea against invading conjugative plasmids or bacteriophages. central to this immunity system are genomic crispr loci that contain fragments of invading dna. these are maintained as spacers in the crispr loci between direct repeats and the spacer composition in any bacterium reflects its evolutionary history. we analysed the crispr locus sequences of 335 yersinia ...	2015	25712141
differential regulation of the hmscde operon in yersinia pestis and yersinia pseudotuberculosis by the rcs phosphorelay system.	yersinia pestis, the agent of plague, forms a biofilm in its flea vector to enhance transmission. y. pestis biofilm development is positively regulated by hmst and hmsd, encoding diguanylate cyclases (dgcs) involved in synthesis of the bacterial second messenger c-di-gmp. rcsa, encoding an auxiliary protein in rcs phosphorelay, is nonfunctional in y. pestis, while in yersinia pseudotuberculosis, rcsa is functional and represses biofilms. previously we showed that rcs phosphorelay negatively regu ...	2015	25672461
variants of glucose-6-phosphate dehydrogenase (g-6-pd) associated with g-6-pd deficiency in puerto ricans.		2015	4728291
variants of glucose-6-phosphate dehydrogenase (g-6-pd) associated with g-6-pd deficiency in puerto ricans.		2015	4728291
personality and psychiatric treatment expectancies.		2015	4683029
[some peculiarities of the clinico-radiographic symptomatology of malignant tumors of the maxilla].		2015	4607876
carcinoma of the gallbladder.		2015	4325325
[dermatological study of a new topical compound of fluocinonide and neomycin sulfate].		2015	4600041
evaluation of the role of the opggh operon in yersinia pseudotuberculosis and its deletion during the emergence of yersinia pestis.	the opggh operon encodes glucosyltransferases that synthesize osmoregulated periplasmic glucans (opgs) from udp-glucose, using acyl carrier protein (acp) as a cofactor. opgs are required for motility, biofilm formation, and virulence in various bacteria. opgh also sequesters ftsz in order to regulate cell size according to nutrient availability. yersinia pestis (the agent of flea-borne plague) lost the opggh operon during its emergence from the enteropathogen yersinia pseudotuberculosis. when ex ...	2015	26150539
type vi secretion system transports zn2+ to combat multiple stresses and host immunity.	type vi secretion systems (t6sss) are widespread multi-component machineries that translocate effectors into either eukaryotic or prokaryotic cells, for virulence or for interbacterial competition. herein, we report that the t6ss-4 from yersinia pseudotuberculosis displays an unexpected function in the transportation of zn2+ to combat diverse stresses and host immunity. environmental insults such as oxidative stress induce the expression of t6ss-4 via oxyr, the transcriptional factor that also r ...	2015	26134274
yersinia pseudotuberculosis ip32953 survives and replicates in trophozoites and persists in cysts of acanthamoeba castellanii.	yersinia pseudotuberculosis is a foodborne enteric pathogen that causes a mild self-limiting diarrhea in humans. yersinia pseudotuberculosis is able to persist in soil and water and in association with fresh produce, but the mechanism by which it persists is unknown. it has been shown that y. pseudotuberculosis co-occurs with protozoans in these environments; therefore, this study investigates if bacterivorous free-living amoeba (fla) are able to support persistence of y. pseudotuberculosis. coc ...	2015	26025069
reprogramming of yersinia from virulent to persistent mode revealed by complex in vivo rna-seq analysis.	we recently found that yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. we performed in vivo rna-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. comprehensive analysis of mixed rna populations from infected tissues revealed that y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of t3ss virulence genes during persistence whe ...	2015	25590628
enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of perforin-2.	perforin-2 (mpeg1) is an effector of the innate immune system that limits the proliferation and spread of medically relevant gram-negative, -positive, and acid fast bacteria. we show here that a cullin-ring e3 ubiquitin ligase (crl) complex containing cullin-1 and βtrcp monoubiquitylates perforin-2 in response to pathogen associated molecular patterns such as lps. ubiquitylation triggers a rapid redistribution of perforin-2 and is essential for its bactericidal activity. enteric pathogens such a ...	2015	26418746
[bacterial recombinant l-asparaginases: properties, structure and anti-proliferative activity].	for more than 40 years l-asparaginases are used in combined therapy of acute lymphoblastic leukemia in children and the range of tumors sensitive to these enzymes constantly extends. this review summarizes results of studies aimed at creation of new systems for heterological expression of bacterial l-asparaginases as erwinia carotovora (ewa), helicobacter pylori (hpa), yersinia pseudotuberculosis (ypa) and rhodospirillum rubrum (rra); special attention is paid to isolation of purified enzymes an ...	2015	26215408
the supramolecular structure of lps-chitosan complexes of varied composition in relation to their biological activity.	the complexes of chitosan (ch) with lipopolysaccharides (lpss) from escherichia coli o55:b5 (e-lps) and yersinia pseudotuberculosis 1b 598 (y-lps) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. the alteration of ζ-potential of e-lps-ch complexes from negative to positive values depending on ch content was detected. the y-lps-ch complexes had similar positive ζ-potentials regardless of ch content. ...	2015	25843841
[cross-immunogenicity of various bacterial l-asparaginases].	evaluate immune response in mice against various l-asparaginases and determine their cross-immunogenicity.	2015	25816523
adhesive properties of yapv and paralogous autotransporter proteins of yersinia pestis.	yersinia pestis is the causative agent of plague. this bacterium evolved from an ancestral enteroinvasive yersinia pseudotuberculosis strain by gene loss and acquisition of new genes, allowing it to use fleas as transmission vectors. infection frequently leads to a rapidly lethal outcome in humans, a variety of rodents, and cats. this study focuses on the y. pestis kim yapv gene and its product, recognized as an autotransporter protein by its typical sequence, outer membrane localization, and am ...	2015	25690102
the adhesive protein invasin of yersinia pseudotuberculosis induces neutrophil extracellular traps via β1 integrins.	yersinia pseudotuberculosis adhesive protein invasin is crucial for the bacteria to cross the intestine epithelium by binding to β1 integrins on m-cells and gaining access to the underlying tissues. after the crossing invasin can bind to β1 integrins on other cell surfaces, however effector proteins delivered by the type iii secretion system y. pseudotuberculosis efficiently inhibit potential immune responses induced by this interaction. here, we use mutant y. pseudotuberculosis strains lacking ...	2015	25576025
roles of rpos in yersinia pseudotuberculosis stress survival, motility, biofilm formation and type vi secretion system expression.	rpos (σ(s)), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. however, the role of rpos appears to differ in different bacteria. while rpos is an important regulator of flagellum biosynthesis, it is associated with biofilm development in edwardsiella tarda. biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. the type vi secretion syst ...	2015	26310305
design, synthesis and evaluation of novel polypharmacological antichlamydial agents.	discovery of new polypharmacological antibacterial agents with multiple modes of actions can be an alternative to combination therapy and also a possibility to slow development of antibiotic resistance. in support to this hypothesis, we synthesized 16 compounds by combining the pharmacophores of chlamydia trachomatis inhibitors and inhibitors of type iii secretion (t3s) in gram-negative bacteria. in this study we have developed salicylidene acylhydrazide sulfonamides (11c &11d) as new antichlamy ...	2015	26204507
conventional and molecular methods in the diagnosis of community-acquired diarrhoea in children under 5 years of age from the north-eastern region of poland.	the purpose of this study was to determine the main causative agents of community-acquired acute diarrhoea in children using conventional methods and pcr.	2015	26159845
[identification of zoonotic bacterial pathogens by the maldi tof ms method].	to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work.	2015	26312374
a lysr-type transcriptional regulator, rovm, senses nutritional cues suggesting that it is involved in metabolic adaptation of yersinia pestis to the flea gut.	yersinia pestis has evolved as a clonal variant of yersinia pseudotuberculosis to cause flea-borne biofilm-mediated transmission of the bubonic plague. the lysr-type transcriptional regulator, rovm, is highly induced only during y. pestis infection of the flea host. rovm homologs in other pathogens regulate biofilm formation, nutrient sensing, and virulence; including in y. pseudotuberculosis, where rovm represses the major virulence factor, rova. here the role that rovm plays during flea infect ...	2015	26348850
diversification of β-augmentation interactions between cdi toxin/immunity proteins.	contact-dependent growth inhibition (cdi) is a widespread mechanism of inter-bacterial competition mediated by the cdib/cdia family of two-partner secretion proteins. cdia effectors carry diverse c-terminal toxin domains (cdia-ct), which are delivered into neighboring target cells to inhibit growth. cdi(+) bacteria also produce cdii immunity proteins that bind specifically to cognate cdia-ct toxins and protect the cell from auto-inhibition. here, we compare the structures of homologous cdia-ct/c ...	2015	26449640
natural killer cells mediate protection against yersinia pseudotuberculosis in the mesenteric lymph nodes.	natural killer cells play a crucial role in the initial defense against bacterial pathogens. the crosstalk between host cells infected with intracellular pathogens and nk cells has been studied intensively, but not much attention has been given to characterize the role of nk cells in the response to extracellular bacterial pathogens such as yersiniae. in this study we used antibody-mediated nk cell depletion to address the importance of this immune cell type in controlling a y. pseudotuberculosi ...	2015	26296209
a new anti-erythrocyte antibody in the duffy system: anti-fy4.		2015	4691232
[independence of identification of the orientation of an object and its location in the visual field].		2015	4663320
[mycobacterial experimental infection and reinfection. conclusion].		2015	4681044
surgical management of acquired tricuspid valve disease.		2015	4587627
a simplified technique for resecting aortic arch aneurysms distal to the innominate artery. a case report.		2015	4572520
[fundamental studies on a method of evaluating the acid resistance of the surface enamel in vivo; influence of cleaning conditions of the tooth surface and the method using non-woven paper plate containing lactate buffer solution (author's transl)].		2015	4534638
urinary tract infection in children.		2015	4489752
development of auxiliary personnel in dentistry for latin america.		2015	4510190
[surgical correction of postoperative diseases].		2015	4661780
the leichardt women's community health centre.		2015	4496699
[bleeding of duodenal varices in portal hypertension].		2015	4542690
standardized multisegmental measurements of blood pressure for quantitative evaluation of the circulation in the limbs.		2015	4358004