Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antigens of fusarium solani: types and criteria for immunochemical characterization. | there are no established methods for the preparation and standardization of fusarium solani antigens. this lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests. | 2000 | 11270086 |
| antifungal activity of bacillus sp. isolated from compost. | four strains of bacillus isolated from lupine compost exhibited an antifungal activity against six plant fungal pathogens (rhizoctonia solani, bipolaris sorokiniana, sclerotinia sclerotiorum, trichothecium roseum, fusarium solani, fusarium oxysporum). it was significantly influenced by the composition of the cultivation media. | 2000 | 11501422 |
| effect of salts and surfactants on the partitioning of fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch. | an aqueous two-phase system composed by a thermoseparating random copolymer of ethylene oxide/propylene oxide 50/50 (%w/w), breox, and hydroxypropyl starch-reppal pes 100 was evaluated for the partitioning of fusarium solani pisi recombinant cutinase. the effect of several additives on the partitioning of pure cutinase was evaluated. micelles of sodium dodecanoate provided a ten-fold increase of the partitioning coefficient (k=9) and recovery yields of 60-75%. the phase diagrams of the systems c ... | 2000 | 11321518 |
| scale-up of recombinant cutinase recovery by whole broth extraction with peg-phosphate aqueous two-phase. | a whole broth extraction using an aqueous two-phase system (atps) composed by 5% (w/w) peg 3350 and 15% (w/w) phosphate was used for the scale-up extraction and isolation of a recombinant fusarium solani pisi cutinase, an extracellular mutant enzyme expressed in saccharomyces cerevisiae, containing a fusion peptide (wp)4. the experiments were carried out at three different scales (10 ml, 1 l and 30 l). mixing time and stirrer speed were evaluated at lab scale (1 l) with two different system comp ... | 2000 | 11321521 |
| fusarium infections of the skin. | fusarium species are ubiquitous and may be found in the soil, air and on plants. fusarium species can cause mycotoxicosis in humans following ingestion of food that has been colonized by the fungal organism. in humans, fusarium species can also cause disease that is localized, focally invasive or disseminated. the pathogen generally affects immunocompromised individuals with infection of immunocompetent persons being rarely reported. localized infection includes septic arthritis, endophthalmitis ... | 2000 | 11964778 |
| [two cases of scalp wound colonization and respiratory tract by mycelial fungi]. | one case of colonization of a post-traumatic scalp wound by aspergillus nidulans and fusarium solani in a healthy adult patient is discussed. a second case of respiratory colonization by acrophialophora fusispora in a child with cystic fibrosis is presented. both cases are reported to illustrate the need of combined histologic and mycologic studies in evaluating patients in order to distinguish between colonization and invasion. | 2000 | 15762812 |
| molecular differentiation of fusarium solani f. sp. glycines from other f. solani based on mitochondrial small subunit rdna sequences. | fusarium solani is a soilborne plant pathogen that infects many different hosts. within the species, there is some specialization, and a number of forma specialis have been described based on host affiliation. one of these, f. solani f. sp. glycines, infects soybean and causes sudden death syndrome. to differentiate between f. solani f. sp. glycines and other f. solani isolates, a partial sequence of the mitochondrial small subunit (mtssu) rrna gene was amplified by polymerase chain reaction and ... | 2000 | 18944555 |
| effects of herbicides on fusarium solani f. sp. glycines and development of sudden death syndrome in glyphosate-tolerant soybean. | abstract sudden death syndrome of soybean, caused by fusarium solani f. sp. glycines, is a disease of increasing economic importance in the united states. although the ecology of sudden death syndrome has been extensively studied in relation to crop management practices such as tillage, irrigation, and cultivar selection, there is no information on the effects of herbicides on this disease. three herbicides (lactofen, glyphosate, and imazethapyr) commonly used in soybean were evaluated for their ... | 2000 | 18944572 |
| viability of heterodera glycines exposed to fungal filtrates. | filtrates from nematode-parasitic fungi have been reported to be toxic to plant-parasitic nematodes. our objective was to determine the effects of fungal filtrates on second-stage juveniles and eggs of heterodera glycines. eleven fungal species that were isolated from cysts extracted from a soybean field in florida were tested on j2, and five species were tested on eggs in vitro. each fungal species was grown in czapek-dox broth and malt extract broth. no toxic activity was observed for fungi gr ... | 2000 | 19270965 |
| breeding for highly fertile isolates of nectria haematococca mpvi that are highly virulent on pea and in planta selection for virulent recombinants. | abstract the heterothallic ascomycete nectria haematococca mating population vi (anamorph fusarium solani) is a broad host range pathogen. field isolates of this fungus that are pathogenic on pea tend to be female sterile, of low fertility, and the same mating type (mat-1), whereas female fertile isolates of either mating type that are highly fertile tend to be nonpathogenic on this plant. to facilitate genetic analysis of traits that may be important in the ability of n. haematococca to parasit ... | 2001 | 18944283 |
| characterization of a 20 kda dnase elicitor from fusarium solani f. sp. phaseoli and its expression at the onset of induced resistance in pisum sativum. | summary dnase released from fusarium solani f. sp. phaseoli (fsph dnase) has previously been reported to induce pathogenesis-related (pr) genes, phytoalexin accumulation and disease resistance against subsequent challenge with the true pea pathogen, fusarium solani f. sp. pisi (fspi). this report is a further analysis of dnase production with probes specific for both the gene and protein. n-terminal analysis of the approximately 20 kda fsph dnase protein facilitated both the development of anti- ... | 2001 | 20573002 |
| fusarium solani fungemia in a patient with acute lymphoblastic leukemia. | a patient with all on anticancer chemotherapy developed fever which was later attributed to be due to fusarium fungemia. the details of the case & a review of literature follows. | 2001 | 14758881 |
| bioremediation of ddt in soil by genetically improved strains of soil fungus fusarium solani. | bioremediation of ddt in soil by genetically improved recombinants of the soil fungus fusarium solani was studied. the parent strains were isolated from soil enriched with ddd or dde (immediate anaerobic and aerobic degradation products of ddt), as further degradation of these products are slow processes compared to the parent compound. these naturally occurring strains isolated from soil, however, are poor degraders of ddt and differed in their capability to degrade its metabolites such as ddd, ... | 2001 | 11826906 |
| disinfection efficacy of contact lens care solutions against ocular pathogens. | three commercially available products labeled as multi-purpose contact lens solutions, one multi-purpose disinfecting solution, and a hydrogen peroxide system were evaluated for antimicrobial activity according to the current international organization for standardization (iso) and the u.s. food and drug administration (fda) stand-alone procedure for disinfecting products. one multi-purpose solution was selected to assess its antimicrobial activity against two human corneal isolates of pseudomon ... | 2001 | 11215601 |
| the thermal stability of the fusarium solani pisi cutinase as a function of ph. | we have investigated the thermal stability of the fusarium solani pisi cutinase as a function of ph, in the range from ph 2-12. its highest enzymatic activity coincides with the ph-range at which it displays its highest thermal stability. the unfolding of the enzyme as a function of ph was investigated by microcalorimetry. the ratio between the calorimetric enthalpy (deltah(cal)) and the van't hoff enthalpy (deltah(v)) obtained, is far from unity, indicating that cutinase does not exhibit a simp ... | 2001 | 12488611 |
| damping-off in conifer seedling nurseries in noshahr and kelardasht. | to study the damping-off of conifer seedlings, we have collected samples from the roots of conifer seedlings (pinus nigra, picea excelsa, abieces spp, cupressus arizonica, cupressus sempervirens) from nurseries in the south of iran (noshahr and kelardasht). after disinfecting the samples, we have used standard media like pda, ma and cla. the following fungi were identified: fusarium solani, fusarium oxysporum, fusarium sambucinum, clamydosporium, rhizoctonia solani, cylindrocarpon spp., alternar ... | 2001 | 12425024 |
| engineering the ph-optimum of a triglyceride lipase: from predictions based on electrostatic computations to experimental results. | the optimisation of enzymes for particular purposes or conditions remains an important target in virtually all protein engineering endeavours. here, we present a successful strategy for altering the ph-optimum of the triglyceride lipase cutinase from fusarium solani pisi. the computed electrostatic ph-dependent potentials in the active site environment are correlated with the experimentally observed enzymatic activities. at ph-optimum a distinct negative potential is present in all the lipases a ... | 2001 | 11334666 |
| [ocular parasitoses and mycoses: cases diagnosed in the central university hospital of sfax between 1996 and 1999]. | parasitical and fungal ophthalmic infections are polymorphic and variably severe. they are rarely reported in publications. the aim of our study has been to specify the parasitic and fungal agents isolated from the ocular samples in our district (sfax-tunisia). we surveyed retrospectively the different ocular parasitosis and mycosis diagnosed in our laboratory (sfax hospital) over a 4 year-period (1996-1999). fungal ophthalmic infections were dominated by corneal localisations: 20 cases of kerat ... | 2001 | 11346972 |
| cloning and expression analysis of nhl1, a gene encoding an extracellular lipase from the fungal pea pathogen nectria haematococca mp vi (fusarium solani f. sp. pisi) that is expressed in planta. | the filamentous fungus nectria haematococca (anamorph fusarium solani f. sp. pisi) resides in soil, and attacks pea seedlings in the area of the underground epicotyl and upper tap root, causing foot rot disease. we detected lipase activity during in vitro growth of n. haematococca. subsequently, a lipase gene was cloned and functionally characterised by heterologous expression in saccharomyces cerevisiae. the full-length cdna of 1152 bp was cloned using a 3' race-pcr approach coupled with cdna l ... | 2001 | 11361331 |
| fusarium solani major allergen peptide iv-1 binds ige but does not release histamine. | fusarium solani (fs) is an important allergen source afflicting 4% of the nasobronchial allergy patients. fus s i3596*, a 65 kda major glycoprotein allergen of fs reacts with 95% fungus sensitive patients. | 2001 | 11422158 |
| identification of a putative solanum tuberosum transcriptional coactivator up-regulated in potato tubers by fusarium solani f. sp. eumartii infection and wounding. | coadaptors or coactivators are a new class of transcription factors capable of interconnecting a regulator dna-binding protein with a component of the basal transcription machinery allowing transcriptional activation to proceed. we report the identification of a novel solanum tuberosum ssp. tuberosum putative transcription coactivator, named stmbf1 (solanum tuberosum multiprotein bridging factor 1). the stmbf1 cdna was isolated from a fusarium solani f. sp. eumartii-infected potato tuber cdna li ... | 2001 | 11454227 |
| detection of microbial volatile organic compounds (mvocs) produced by moulds on various materials. | twelve fungal species were screened for microbial volatile organic compounds (mvocs): aspergillus fumigatus, a. versicolor, a. niger, a. ochraceus, trichoderma harzianum, t. pseudokoningii, penicillium brevicompactum, p. chrysogenum, p. claviforme, p. expansum, fusarium solani and mucor sp. more than 150 volatile substances derived from fungal cultures have been analysed by head-space solid-phase microextraction (hs-spme). each species had a defined mvoc profile which may be subjected to conside ... | 2001 | 11759153 |
| direct product sequestration of a recombinant cutinase from batch fermentations of saccharomyces cerevisiae. | the recovery of cutinase of fusarium solani pisi produced by the yeast saccharomyces cerevisiae was studied in a fluidised bed adsorption system directly integrated with a productive fermenter (so-called direct product sequestration; dps). the relative efficiency of this system was compared with the one of a conventional purification process by discrete sequences of fermentation, broth clarification, ultrafiltration and fixed bed anion exchange chromatography. by direct product sequestration of ... | 2001 | 11787802 |
| in vitro antifungal activities of the new triazole ur-9825 against clinically important filamentous fungi. | we used a modified reference microdilution method (the m-38p method) to evaluate the in vitro activities of the new triazole ur-9825 in comparison with those of amphotericin b against 77 strains of opportunistic filamentous fungi. ur-9825 was clearly more active than amphotericin b against all fungi except fusarium solani and scytalidium spp. notably, ur-9825 had low mics for aspergillus fumigatus and paecilomyces lilacinus (mics at which 90% of isolates are inhibited, 0.125 microg/ml for both s ... | 2001 | 11502542 |
| dsc studies of fusarium solani pisi cutinase: consequences for stability in the presence of surfactants. | the application of cutinase from fusarium solani pisi as a fat-stain removing ingredient in laundry washing is hampered by its lack of stability in the presence of anionic surfactants. we postulate that the stability of cutinase towards anionics can be improved by mutations increasing its temperature stability. thermal unfolding as measured with dsc, appears to be irreversible, though the thermograms are more symmetric than predicted by a simple irreversible model. in the presence of taurodeoxyc ... | 2001 | 11527580 |
| use of topical clotrimazole in human keratomycosis. | twelve out of 15 patients (9 males and 6 females) with laboratory-proven fungal keratitis were treated with topical clotrimazole, a synthetic imidazole derivative. their ages ranged from 8 to 70 years. the posttreatment visual acuities were significantly better than the pretreatment visual acuities. no significant biomicroscopic signs of ocular surface toxicity were noted. the ocular condition in 3 patients (all infected with fusarium solani) deteriorated to blindness despite the antifungal ther ... | 2001 | 11528261 |
| fusariosis associated with pathogenic fusarium species colonization of a hospital water system: a new paradigm for the epidemiology of opportunistic mold infections. | we sought the reservoir of fusarium species in a hospital with cases of known fusarial infections. cultures of samples from patients and the environment were performed and evaluated for relatedness by use of molecular methods. fusarium species was recovered from 162 (57%) of 283 water system samples. of 92 sink drains tested, 72 (88%) yielded fusarium solani; 12 (16%) of 71 sink faucet aerators and 2 (8%) of 26 shower heads yielded fusarium oxysporum. fusarium solani was isolated from the hospit ... | 2001 | 11692299 |
| synergy, pharmacodynamics, and time-sequenced ultrastructural changes of the interaction between nikkomycin z and the echinocandin fk463 against aspergillus fumigatus. | we investigated the potential synergy between two cell wall-active agents, the echinocandin fk463 (fk) and the chitin synthase inhibitor nikkomycin z (nz), against 16 isolates of filamentous fungi. susceptibility testing was performed with a broth macrodilution procedure by nccls methods. the median minimal effective concentration (mec) of fk against all aspergillus species was 0.25 microg/ml (range, 0.05 to 0.5 microg/ml). for fusarium solani and rhizopus oryzae, mecs of fk were >512 microg/ml. ... | 2001 | 11709302 |
| [activity of itraconazole against clinical isolates of aspergillus spp. and fusarium spp. determined by the m38-p nccls method]. | the antifungal activity of itraconazole was studied in 101 clinical isolates of aspergillus fumigatus, a. flavus, a. niger, a. terreus, a. nidulans, a. candidus, a. glaucus, a. clavatus, fusarium solani, f. oxysporum and f. semitectum. the minimum inhibitory concentrations (mic) were determined according to the protocol of the m38-p national committee for laboratory standards (nccls) document using a microdilution method in 1640 rpmi liquid medium (visual reading at 48 and 72 h incubation). in g ... | 2001 | 11753450 |
| enzymatic, clinical and histologic evaluation of corneal tissues in experimental fungal keratitis in rabbits. | mycotic keratitis, being frequently refractive to most of the currently available antifungal therapy, continues to pose a therapeutic challenge to the clinician. in keratitis of infectious etiology stromal dissolution may be brought about by a combination of agent and host factors. an understanding of the source and nature of corneal tissue damage is essential for evolving more effective therapeutic modalities in the treatment of fungal keratitis. in the present study, we have characterized the ... | 2001 | 11273671 |
| comparison of the e-test with the nccls m38-p method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. | the national committee for clinical laboratory standards (nccls) m38-p method describes standard parameters for testing the fungistatic antifungal activities (mics) of established agents against filamentous fungi (molds). the present study evaluated the in vitro fungistatic activities of itraconazole and amphotericin b by the e-test and the nccls m38-p microdilution method against 186 common and emerging pathogenic molds (123 isolates of aspergillus spp. [five species], 16 isolates of fusarium s ... | 2001 | 11283057 |
| phase ii study of 4-ipomeanol, a naturally occurring alkylating furan, in patients with advanced hepatocellular carcinoma. | 4-ipomeanol (ipo; nsc 394438), a naturally occurring furan isolated from common sweet potatoes (ipomoea batatas) infected with the fungus fusarium solani was the first agent to be developed by the national cancer institute based on a biochemical-biological rationale as an anticancer agent targeted specifically against lung cancer. prior to clinical development, ipo was shown to induce pulmonary toxicity in the lungs of several mammalian species because the agent is metabolized to a highly reacti ... | 2001 | 11291834 |
| polymicrobial keratitis after laser in situ keratomileusis. | to report a case of polymicrobial infectious keratitis in one eye of a patient who had undergone bilateral simultaneous laser in situ keratomileusis (lasik). | 2001 | 11310765 |
| effect of culture conditions on lipase production by fusarium solani in batch fermentation. | lipase (glycerol ester hydrolase ec 3.1.1.3.) from a brazilian strain of fusarium solani fsi has been investigated. the effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. lipase specific activity was highest (0.45 u mg(-1)) for sesame oil. when this medium was supplemented with trace elements using olive oil, corn oil and sesame oil the lipase specific activity increased to 0.86, 1.89 and 1.64 u mg(-1), respective ... | 2001 | 11315806 |
| multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 a. | acetylxylan esterase (axeii; 207 amino acids) from penicillium purpurogenum has substrate specificities toward acetate esters of d-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. the crystal structure of axeii has been determined by single isomorphous replacement and anomalous scattering, and refined at 0.90- and 1.10-a resolutions with data collected at 85 k and 295 k, respectively. the tertiary structure consists of a doubly wound alpha/beta sandwich, having ... | 2001 | 11134051 |
| a comparison of the effects of dna-damaging agents and biotic elicitors on the induction of plant defense genes, nuclear distortion, and cell death. | pea (pisum sativum l. cv alcan) endocarp tissue challenged with an incompatible fungal pathogen, fusarium solani f. sp. phaseoli or fungal elicitors results in the induction of pathogenesis-related (pr) genes and the accumulation of pisatin, a phytoalexin. essentially the same response occurs in pea tissue exposed to dna-specific agents that crosslink or intercalate dna. in this study, the effects of dna-damaging agents were assessed relative to the inducible expression of several pea pr genes: ... | 2001 | 11161032 |
| induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively). | cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ... | 2001 | 11164297 |
| a continuous membrane bioreactor for ester synthesis in organic media: i. operational characterization and stability. | the feasibility of continuous ester synthesis in a membrane bioreactor (mbr) by a recombinant cutinase from fusarium solani pisi was investigated, using the optimal conditions previously attained by medium engineering. the objective was to analyze the mbr behavior as a differential or an integral reactor. the main component of the reactor was an anisotropic ceramic membrane with 15,000 nmwco. the operating variables included the influence of substrates ratio and flow rate on the conversion degre ... | 2001 | 11114650 |
| a continuous membrane bioreactor for ester synthesis in organic media: ii. modeling of mbr continuous operation. | a model was developed to describe the conversion degree in a membrane bioreactor (mbr) for the synthesis of short-chain esters as a function of the flow rate. the transesterification reaction was catalyzed by a recombinant cutinase of fusarium solani pisi microencapsulated in reversed micelles of aot/isooctane. the differences of product concentration in permeate and retentate together with the deactivation profiles led to an enzyme distribution evaluation that describes the experimental values ... | 2001 | 11114651 |
| comparative antimicrobial efficacy of multi-purpose hydrogel lens care solutions. | ten single-bottle multipurpose hydrogel lens care solutions commercially available in the european market were evaluated for antimicrobial efficacy using the international organization for standardization's (iso) stand-alone procedure. the results of this study indicate that renu multi plus renu multi purpose, complete and dua meet the iso stand-alone primary acceptance criteria for stand-alone disinfectants against all challenge organisms: staphylococcus aureus, serratia marcescens, pseudomonas ... | 2001 | 16303461 |
| regulation of constitutively expressed and induced cutinase genes by different zinc finger transcription factors in fusarium solani f. sp. pisi (nectria haematococca). | cutin monomers, generated by the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate into the host through the cuticle whose major structural polymer is cutin. we cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from fusarium solani f. pisi (nectria haematococca). amino acid sequence deduced from the nucleotide sequence of cut1 and cut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, r ... | 2002 | 11756444 |
| mitosis and motor proteins in the filamentous ascomycete, nectria haematococca, and some related fungi. | among filamentous fungi, mitosis has been studied in-depth in just a few species. the mitotic apparatuses in the ascomycetous fusarium spp. are the most clearly and readily visualized in vivo within this group; fluorescent labeling is unnecessary. this superior cytological tractability has enabled detailed studies and revealing experiments that have led the way toward a more complete understanding of fungal mitosis. some of the most important discoveries include the role of half-spindles in deve ... | 2002 | 11804038 |
| evaluation of lectin staining in the diagnosis of fungal keratitis in an experimental rabbit model. | to assess the sensitivity, specificity, and reliability of peroxidase labeled lectin staining in the diagnosis of fungal keratitis in an experimental rabbit model. | 2002 | 11815751 |
| resistance locus pyramids alter transcript abundance in soybean roots inoculated with fusarium solani f.sp. glycines. | soybean sudden death syndrome (sds) is caused by fusarium solani f.sp. glycines (fsg). six quantitative trait loci (qtls), each conferring partial resistance to sds, have been discovered in an essex x forrest recombinant inbred line (ril) population, but their mode of action is not clear. this study aimed to identify genes (ests) whose mrna transcripts were altered in abundance in soybean roots following inoculation of fsg. roots of the soybean variety forrest (four resistance alleles) were inoc ... | 2002 | 12436262 |
| partitioning of peptide-tagged proteins in aqueous two-phase systems using hydrophobically modified micelle-forming thermoseparating polymer. | genetic engineering has been used to construct hydrophobically modified fusion proteins of cutinase from fusarium solani pisi and tryptophan-containing peptides. the aim was to enhance the partitioning of the tagged protein in a novel aqueous two-phase system formed by only one water-soluble polymer. the system was based on a hydrophobically modified random copolymer of ethylene oxide (eo) and propylene oxide (po) units, hm-eopo, with myristyl groups (c(14)h(29)) at both ends. the hm-eopo polyme ... | 2002 | 12445475 |
| complete sequence of the genome of two dsrna viruses from discula destructiva. | complete nucleotide sequences were determined for the four dsrna segments present in isolate 247 of discula destructiva from south carolina. the largest dsrna (dsrna 1) was 1787 bp in length with a single open reading frame (orf) that coded for a putative rna-dependent rna polymerase (rdrp). the dsrna 2 was 1585 bp in length with a single orf that coded for a putative viral coat protein. both the dsrna 3 (1178 bp in length) and dsrna 4 (308 bp) contained single orfs. however, neither the nucleot ... | 2002 | 12457976 |
| common loci underlie field resistance to soybean sudden death syndrome in forrest, pyramid, essex, and douglas. | soybean [ glycine max (l.) merr.] sudden death syndrome (sds) caused by fusarium solani f. sp. glycines results in severe yield losses. resistant cultivars offer the most-effective protection against yield losses but resistant cultivars such as 'forrest' and 'pyramid' vary in the nature of their response to sds. loci underlying sds resistance in 'essex' x forrest are well defined. our objectives were to identify and characterize loci and alleles that underlie field resistance to sd ... | 2002 | 12582700 |
| a pyramid of loci for partial resistance to fusarium solani f. sp. glycines maintains myo-inositol-1-phosphate synthase expression in soybean roots. | myo-inositol 1-phosphate synthase (mips; ec 5.5.1.4) converts glucose 6-phosphate to myo-inositol 1-phosphate in the presence of nad(+). it catalyzes the first step in the synthesis of myo-inositol and pinitol, and is a rate limiting step in the de novo biosynthesis of inositol in eukaryotes. therefore, mips is involved in biotic and abiotic stress via ca(2+) signalling. seedlings of four soybean genotypes were inoculated with fusarium solani f. sp. glycines, the causative agent of sudden death ... | 2002 | 12582889 |
| pyrene degradation by yeasts and filamentous fungi. | the saprotrophic soil fungi fusarium solani (mart.) sacc., cylindrocarpon didymum (hartig) wollenw, penicillium variabile sopp. and the yeasts rhodotorula glutinis (fresenius) harrison and rhodotorula minuta (saito) harrison were cultured in mineral medium with pyrene. the remaining pyrene concentrations were periodically determined during 20 incubation days, using hplc. to assess the metabolism of pyrene degradation we added 0.1 microci of [4,5,9,10] 14c-pyrene to each fungi culture and measure ... | 2002 | 11843531 |
| high probability of disrupting a disulphide bridge mediated by an endogenous excited tryptophan residue. | it is well known that ultraviolet (uv) radiation may reduce or even abolish the biological activity of proteins and enzymes. uv light, as a component of sunlight, is illuminating all light-exposed parts of living organisms, partly composed of proteins and enzymes. although a considerable amount of empirical evidence for uv damage has been compiled, no deeper understanding of this important phenomenon has yet emerged. the present paper presents a detailed analysis of a classical example of uv-ind ... | 2002 | 11847281 |
| [an autopsy case of ph1--positive acute lymphoblastic leukemia with disseminated infection of fusarium solani]. | a 56-year-old woman with ph1--positive acute lymphoblastic leukemia was admitted to our hospital for induction chemotherapy in june 1999. the patient was presented with a central scotoma of left eye during treatment course and was given diagnosis of endophthalmitis. thereafter she also developed skin induration and suffered from serious pneumonia. amphotericin b administration was started because of high titer of beta-d-glucan, but soon discontinued due to its adverse effect. blood cultures yiel ... | 2002 | 11852477 |
| cutinase-peptide fusions in thermoseparating aqueous two-phase systems. prediction of partitioning and enhanced tag efficiency by detergent addition. | it is of increasing importance to develop efficient purification methods for recombinant proteins where the number of steps can be minimised. the aim has been to establish a method for predicting the partitioning of the wild-type target protein in an aqueous two-phase system, and with this as basis, develop fusion tags and optimise the phase system for enhanced partitioning of the target protein. the surface of the lipolytic enzyme cutinase from fusarium solani pisi was investigated with a compu ... | 2002 | 11873963 |
| mycetoma caused by fusarium solani with osteolytic lesions on the hand: case report. | eumycetoma is a mycotic disease caused by saprophytic soil fungi that are usually inoculated through minor injuries. a case of mycetoma in a brazilian farmer aged 71 years is reported. this patient presented erythema and edema on the dorsal surface of the left hand with multiple crusted and cicatricial lesions. no macroscopic grains were observed. the histopathological findings showed grains consisted of numerous hyphae which stained well with gomori-grocott method. this material obtained by cut ... | 2002 | 11913759 |
| fusarium solani keratitis following lasik for myopia. | 2002 | 12234908 | |
| bioactivation of the fungal phytotoxin 2,5-anhydro-d-glucitol by glycolytic enzymes is an essential component of its mechanism of action. | an isolate of fusarium solani, nrrl 18883, produces the natural phytotoxin 2,5-anhydro-d-glucitol (ahg). this fungal metabolite inhibited the growth of roots (150 of 1.6 mm), but it did not have any in vitro inhibitory activity. the mechanism of action of ahg requires enzymatic phosphorylation by plant glycolytic kinases to yield ahg-1,6-bisphosphate (ahg-1,6-bisp), an inhibitor of fru-1,6-bisp aldolase. ahg-1,6-bisp had an i50 value of 570 microm on aldolase activity, and it competed with fru-1 ... | 2002 | 12240991 |
| optimal testing conditions for determining mics and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: nccls collaborative study. | this collaborative three-center study evaluated nccls m38-a document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of aspergillus nidulans, a. terreus, bipolaris hawaiiensis, b. spicifera, cladophialophora bantiana, dactylaria constricta, fusarium solani, paecilomyces lilacinus, scedosporium prolificans, trichoderma longibrachiatum, and wangiella dermatitidis for itraconazole, three new triazoles (voriconazole, posaconazole, and ravucona ... | 2002 | 12354880 |
| substrate-regulated cyanide hydratase (chy) gene expression in fusarium solani: the potential of a transcription-based assay for monitoring the biotransformation of cyanide complexes. | the fungus fusarium solani detoxifies cyanide through induction of the cyanide hydratase gene activity (chy) in the presence of either kcn or the metal-complexed cyanides, k2ni(cn)4 or k4fe(cn)6, at ph 7.0 and 4.0 respectively. sequence analysis of the chy gene identified primers for reverse transcriptase-polymerase chain reaction (rt-pcr)-directed analysis of mrna transcripts, which demonstrated that activity correlated to the substrate-specific induction of gene expression. chy transcription w ... | 2002 | 12000318 |
| diversity in soil fungi from undisturbed and disturbed celtis tala and scutia buxifolia forests in the eastern buenos aires province (argentina). | the rhizospheric soil microfungi from a native forest (undisturbed and disturbed) were studied using soil dilution plate and soil washing methods. fungi were isolated using slightly acid and alkaline culture media. 54 taxa were isolated: 49 from undisturbed forest soil and 37 from disturbed forest soil. acremonium sp., aspergillus ustus, coemansia pectinata, doratomyces stemonitis, fusarium solani, f. oxysporum, gliocladium roseum, humicola fusco-atra, mortierella sp., penicillium lilacinum, tri ... | 2002 | 12002400 |
| in vitro activity of fk463, a novel lipopeptide antifungal agent, against a variety of clinically important molds. | the in vitro antifungal activity of fk463 against a variety of clinically important opportunistic molds was compared with amphotericin b, itraconazole and fluconazole by using the broth microdilution method m27-a specified by the national committee for clinical laboratory standards. fk463 exhibited potent activity against aspergillus species, which was superior to those of all other compounds tested. fk463 was also active against the dematiaceous fungi cladosporium trichoides, exophiala spinifer ... | 2002 | 12011539 |
| alternate conformations observed in catalytic serine of bacillus subtilis lipase determined at 1.3 a resolution. | bacillus subtilis extracellular lipase (bsl) has an exceptionally low molecular weight (19.4 kda) for a member of the lipase family. a crystallographic study was performed on bsl in order to design and produce mutant bsl that will be more suitable for industrial uses based on analysis of the three-dimensional structure. recently, the crystal structure of bsl has been determined at 1.5 a resolution [van pouderoyen et al. (2001). j. mol. biol. 309, 215-226]. in the present study, a new crystal for ... | 2002 | 12077437 |
| host recognition by pathogenic fungi through plant flavonoids. | a common characteristic among fungal pathogens of plants is that each specializes on a narrow range of specific plants as hosts. one adaptation to a specific host plant is the recognition of the host's chemicals which can be used to trigger genes or developmental pathways needed for pathogenesis. the production of characteristic flavonoids by plants, particularly those exuded from roots by legumes, appear to be used as signals for various microbes, including symbionts as well as pathogens. nectr ... | 2002 | 12083470 |
| ocular and systemic posaconazole(sch-56592) treatment of invasive fusarium solani keratitis and endophthalmitis. | 2002 | 12084760 | |
| allergenic cross-reactivity of curvularia lunata with other airborne fungal species. | curvularia lunata is an important fungus for respiratory allergic disorders. previous studies indicated cross-reactivity of curvularia with other fungi. however, the cross-reactive allergenic component (s) were not identified. the present work was carried out to study the shared allergenic components of c. lunata and others. | 2002 | 12100306 |
| canker and wilt of black locust (robinia pseudoacacia l.) caused by fusarium species. | from the pathological material of black locust trees showing symptoms of wilting of the foliage or canker of the bark the following fusarium species were isolated: fusarium avenaceum (fr.) sacc., fusarium lateritium nees., fusarium semitectum berk. & rav., fusarium solani (mart.) sacc., fusarium sulphureum schlecht. (syn.: fusarium sambucinum fuckel f. 6 wollenw.) the results of the provocation infections of one-year-old black locust seedlings showed that all of the species--except fusarium sola ... | 2002 | 12109155 |
| endogenous endophthalmitis following disseminated fungemia due to fusarium solani in a patient with acute myeloid leukemia. | we report the case of a young man with a resistant acute myeloid leukemia (aml) who developed a disseminated fungemia due to fusarium solani involving the skin and lungs, during the neutropenic phase following a chemotherapy course. despite continuous therapy with liposomal amphotericin b, he developed a bilateral endophthalmitis that rapidly evolved to complete blindness. the patient underwent two procedures of vitrectomy, with detection of f. solani in the vitreous fluid, and continued antifun ... | 2002 | 12144539 |
| analysis of phylogenetic relationship of cylindrocarpon lichenicola and acremonium falciforme to the fusarium solani species complex and a review of similarities in the spectrum of opportunistic infections caused by these fungi. | an emerging pattern of similarity in medical case reports led to a project to compare the phylogenetic affinities of two well-known tropical fungal opportunistic pathogens, cylindrocarpon lichenicola and acremonium falciforme, to members of the fusarium solani species complex. c. lichenicola and a. falciforme, despite their deviating conidial morphologies, were shown via sequencing of the ribosomal large subunit to be well instituted within a clade mainly consisting of typical f. solani strains ... | 2002 | 12149344 |
| electrophoretic karyotyping and gene mapping of seven formae speciales in fusarium solani. | chromosomal dnas of 22 strains in 7 formae speciales (f. spp.) of fusarium solani were compared by pulsed field gel electrophoresis (pfge) and gene mapping on the chromosomes. using pfge, complete separation of the full components of the genome was not attained, due to the limited resolution of large chromosomes, but 5-12 chromosomes with sizes of 0.6-5.7 mbp were resolvable for every strain. although each strain had a unique banding profile, similarity in the banding profile was noticed among s ... | 2002 | 12172966 |
| a case of melanonychia caused by fusarium solani. | 2002 | 12207614 | |
| isolation of fusarium solani from a dog: identification by molecular analysis. | a strain of fusarium solani was isolated from a dog showing many cutaneous and submucosal nodules and pyogranulomatous kidney lesions. clinical isolates from this systemic infection were identified using microscopic examination and confirmed by molecular analysis. | 2002 | 12230226 |
| atomic force microscopy study of the interaction of fusarium solani pisi cutinase with lipid surfaces. | we present an atomic force microscopy (afm) study of a supported triacylglyceride multilayer phase and its interaction with lipolytic enzyme cutinase from fusarium solani pisi. the multilayer triacylglyceride phase of coconut oil showed a rippled surface structure in the afm images. upon enzymatic degradation of the triacylglyceride phase, the ripple structure vanished rapidly. the apparent catalytic rate constants could be estimated based on the afm image information. interestingly, in one samp ... | 2002 | 12908312 |
| interaction between root lesion nematode pratylenchus vulnus and two species of fusarium on growth and development of maple seedlings. | producing healthy seedlings and distributing them to far areas, is one of the most important factor for developing forests. because of easy planting, rapid growth and good wood quality, acer velutinum is the most useful species, among the softwood and hardwood trees. growth and development of the nurseries were effected by different pests and diseases, the nematodes are one of these agents. they are not actively surveyed in the forest nurseries in iran. on a survey of maple seedling in the fores ... | 2002 | 12696440 |
| eggs of tylenchulus semipenetrans inhibit growth of phytophthora nicotianae and fusarium solani in vitro. | in previous greenhouse and laboratory studies, citrus seedlings infested with the citrus nematode tylenchulus semipenetrans and later inoculated with the fungus phylophthora nicotianae grew larger and contained less fungal protein in root tissues than plants infected by only the fungus, demonstrating antagonism of the nematode to the fungus. in this study, we determined whether eggs of the citrus nematode t. semipenetrans and root-knot nematode meloidogyne arenaria affected mycelial growth of p. ... | 2002 | 19265943 |
| tylenchulus semipenetrans alters the microbial community in the citrus rhizosphere. | infection of citrus seedlings by tylenchulus semipenetrans was shown to reduce subsequent infection of roots by phytophthora nicotianae and to increase plant growth compared to plants infected by only the fungus. hypothetical mechanisms by which the nematode suppresses fungal development include nutrient competition, direct antibiosis, or alteration of the microbial community in the rhizosphere to favor microorganisms antagonistic to p. nicotianae. a test of the last hypothesis was conducted via ... | 2003 | 19265991 |
| fungal parasitism of heterodera glycines eggs as influenced by egg age and pre-colonization of cysts by other fungi. | the objective of this study was to determine the effect of egg age and pre-colonization of cysts by a saprophytic or parasitic fungus on parasitism of heterodera glycines eggs by other parasitic fungi. in agar and in soil tests, fungi generally parasitized more eggs in early developmental stages than eggs containing a juvenile. the effect of pre-colonization of cysts by a fungus on parasitism of eggs by other fungi depended on the fungi involved. in most cases, pre-colonization of cysts by an un ... | 2003 | 19262761 |
| isotope fractionations in the biosynthesis of cell components by different fungi: a basis for environmental carbon flux studies. | abstract the isotope fractionation of carbon from substrates possessing different isotope ratios into fatty acids of polar lipids and amino acids was determined for four different fungi (rhizopus arrhizus, mortierella isabellina, fusarium solani, aspergillus niger). carbon isotope ratios of fungi closely followed that of the substrates. palmitic acid (c16:0), derived from phospholipids, did not display a large carbon isotope fractionation against the substrate. stearic acid (c18:0), however, was ... | 2003 | 19719589 |
| quantification of fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. | abstract the capacity of the arbuscular mycorrhizal fungus glomus intraradices in reducing the presence of fusarium solani f. sp. phaseoli in bean plants and the surrounding mycorrhizosphere soil was evaluated in a compartmentalized experimental system. quantification of the pathogen and the symbiont in plant tissues, the soil regions of the mycorrhizosphere (rhizosphere and mycosphere), and the bulk soil was accomplished using specific polymerase chain reaction (pcr) primers in real-time pcr as ... | 2003 | 18943138 |
| analysis of pea hmg-i/y expression suggests a role in defence gene regulation. | summary hmg-i/y proteins are characterized by the presence of at-hook motifs, dna binding domains that recognize at-rich tracts of dna. by facilitating protein:protein and protein:dna interactions in the vicinity of these at-rich binding sites, hmg-i/y positively or negatively regulates gene expression. several pea defence gene promoters have at-rich tracts of dna that are potential targets for modulation via hmg-i/y. in this study, a comparison of the expression of a pea defence gene (drr206) m ... | 2003 | 20569385 |
| sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the fusarium solani species complex--f. virguliforme in north america and f. tucumaniae in south america. | soybean sudden-death syndrome has become a serious constraint to commercial production of this crop in north and south america during the past decade. to assess whether the primary etiological agent is panmictic in both hemispheres, morphological and molecular phylogenetic analyses were conducted on strains selected to represent the known pathogenic and genetic diversity of this pathogen. maximum-parsimony analysis of dna sequences from the nuclear ribosomal intergenic spacer region and the sing ... | 2003 | 21148975 |
| antifungal effects of different organic extracts from melia azedarach l. on phytopathogenic fungi and their isolated active components. | extracts from different parts of melia azedarach l. were studied as potential antifungal agents for selected phytopathogenic fungi. in a serial agar dilution method, hexanic and ethanolic extracts from fruit, seed kernels, and senescent leaves exhibited fungistatic activity against aspergillus flavus,diaporthe phaseolorum var. meridionales, fusarium oxysporum, fusarium solani, fusarium verticillioides, and sclerotinia sclerotiorum. both hexanic extract from senescent leaves and ethanolic extract ... | 2003 | 12696928 |
| activity of the amidoamine myristamidopropyl dimethylamine against keratitis pathogens. | microbial keratitis accounts for up to 30% of blindness in some less developed societies. the development of a single broad-spectrum topical antimicrobial effective against bacteria, fungi and acanthamoeba would have a major impact on reducing the morbidity and simplifying the treatment of microbial keratitis. to this end, the activity of the amidoamine myristamidopropyl dimethylamine (mapd) was investigated against common causes of microbial keratitis. | 2003 | 12716783 |
| activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. | the adsorption of a recombinant cutinase from fusarium solani pisi onto the surface of 100 nm diameter poly(methyl methacrylate) (pmma) latex particles was evaluated. adsorption of cutinase is a fast process since more than 70% of protein molecules are adsorbed onto pmma at time zero of experiment, irrespective of the tested conditions. a langmuir-type model fitted both protein and enzyme activity isotherms at 25 degrees c. gamma(max) increased from 1.1 to 1.7 mg m(-2) and u(max) increased from ... | 2003 | 12730007 |
| volatile metabolites from salvia fruticosa as antifungal agents in soilborne pathogens. | the volatile metabolites of salvia fruticosa plants, growing wild in 15 localities scattered across greece, were analyzed by means of gc and gc-ms. the essential oil content ranged from 0.69 to 4.68%, and the results of the analyses showed a noticeable variation in the amounts of the five main components [1,8-cineole, alpha-thujone, beta-thujone, camphor, and (e)-caryophyllene]. the antifungal activities of the essential oils from two localities, belonging in two different groups of cluster and ... | 2003 | 12744657 |
| improving the mouse model for studying the efficacy of voriconazole. | outbred icr mice were rendered neutropenic, infected intravenously with fusarium solani and treated orally with voriconazole. when given alone, voriconazole was not protective up to 40 mg/kg/day. when grapefruit juice was administered before infection, mice were protected by voriconazole. the mechanism may be inhibition of gut mucosal cytochrome enzymes that rapidly degrade voriconazole in the mouse. these murine studies support expansion of voriconazole therapy in other highly resistant systemi ... | 2003 | 12746374 |
| advanced fusarium keratitis progressing to endophthalmitis. | to review the clinical course, treatment, and visual outcomes of keratitis with endophthalmitis caused by the filamentary fungus fusarium. | 2003 | 12792470 |
| a binuclear zinc transcription factor binds the host isoflavonoid-responsive element in a fungal cytochrome p450 gene responsible for detoxification. | the pda1 gene of the filamentous fungus nectria haematococca mpvi (anamorph: fusarium solani) encodes pisatin demethylase, a cytochrome p450. pisatin is a fungistatic isoflavonoid produced by garden pea (pisum sativum), a host for this fungus. pisatin demethylase detoxifies pisatin and functions as a virulence factor for this fungus. pisatin induces pda1 expression both in cultured mycelia as well as during pathogenesis on pea. the regulatory element within pda1 that provides pisatin-responsive ... | 2003 | 12823815 |
| [post traumatic subcutaneous mycosis due to fusarium solani]. | we report the case of a subcutaneous hyalohyphomycosis of a 24-year-old man, a rural worker with an ulcerative lesion in the right leg of approximately one year duration. it was caused by traumatic implantation of a yerba mate branch. the diagnosis was made by direct microscopic examination with 20% potassium hydroxide (koh) and revealed several septate hyaline hyphae. it was confirmed by culture of several samples where fusarium solani was isolated. the patient received local and systemic antif ... | 2003 | 12825979 |
| synthesis and antimicrobial activity of n-(substituted)-n'-(2,3-dihydro-2-oxido-5-benzoyl-1h-1,3,2-benzodiazaphosphol-2-yl) ureas. | n-(substituted)-n'-(2,3-dihydro-5-benzoyl-2-oxido-1h-1,3,2-benzodiazaphosphol-2-yl) ureas were synthesized by reacting 3,4-diaminobenzophenone (4) with different chlorides of carbamidophosphoric acids (3) in the presence of triethylamine at 40-45 degrees c. their 1h-, 13c- and 31p-nmr spectral data are discussed. the title compounds were screened for antifungal and antibacterial activity against the fungi aspergillus niger and fusarium solani and bacteria escherichia coli and staphylococcus aure ... | 2003 | 12843597 |
| non-pathogenic fusarium solani represses the biosynthesis of nematicidal compounds in vitro and reduces the biocontrol of meloidogyne javanica by pseudomonas fluorescens in tomato. | the aim of the present investigation was to determine the influence of various fusarium solani strains on the production of nematicidal agent(s) in vitro and biocontrol of meloidogyne javanica in tomato by pseudomonas fluorescens strains cha0 and cha0/pme3424. | 2003 | 12859651 |
| pentamidine is active in vitro against fusarium species. | fusariosis is an emerging opportunistic mycosis against which currently used antifungals have limited activity. here, we investigated the in vitro activities of pentamidine (pnt) against 10 clinical isolates of fusarium species (five fusarium solani isolates and five non-f. solani isolates) by using the national committee for clinical laboratory standards microdilution method in three different media (rpmi, rpmi-2, and a yeast nitrogen base medium), disk diffusion testing, and viability dye stai ... | 2003 | 14506038 |
| improvement of phenylethanoid glycosides production by a fungal elicitor in cell suspension culture of cistanche deserticola. | when, on the 15th day of growth, an elicitor from fusarium solani was added at 40 mg l(-1) to cistanche deserticola cell suspension cultures, the contents of echinacoside, acteoside and total phenylethanoid glycosides (pegs) in cultured cells all increased over the next 27 d by over 100% to 15 mg g(-1) dry wt, 9 mg g(-1) dry wt and 57 mg g(-1) dry wt, respectively. the final biomass (1.3 mg dry wt ml(-1)) was not affected. | 2003 | 14514046 |
| [phytotoxic activity of chernozem saprophytic micromycetes: specificity, sorption and stability of phytotoxins in soil]. | micromycetes of the complex of typical chernozem saprotrophic fungi released phytotoxic metabolites into medium. the metabolites displayed their phytotoxic activities directly in soil. evaluation of the toxicities, range of biological effects activities, and stabilities of phytotoxins in soil and the rates of their biodegradation allowed the species that can serve as indicators of chernozem microbial toxicosis to be selected, namely, aspergillus clavatus, fusarium solani, talaromyces flavus, pen ... | 2003 | 14520964 |
| successful voriconazole therapy of disseminated fusarium solani in the brain of a neutropenic cancer patient. | 2003 | 14581897 | |
| two unrelated double-stranded rna molecule patterns in gremmeniella abietina type a code for putative viruses of the families totiviridae and partitiviridae. | two double stranded (ds) rna molecule patterns, probably of viral origin, were sequenced from gremmeniella abietina var. abietina type a. the genome of gremmeniella abietina rna virus l1 (garv-l1) from isolate hr2 was 5133 bp and contained two open reading frames (orfs). the 5'-proximal orf coded for a putative coat protein (cp) and the 3'-proximal orf encoded putative rna-dependent rna polymerase (rdrp). garv-l1 had sequence similarities with a previously described totivirus (helminthosporium v ... | 2003 | 14648287 |
| fusarium solani infection complicated by tuberculous omarthritis. | 2003 | 14673299 | |
| isolation and characterization of a lectin from annona muricata seeds. | a lectin with a high affinity for glucose/mannose was isolated from annona muricata seeds (annonaceae) by gel filtration chromatography on sephacryl s-200, ion exchange chromatography on a deae sp-5 pw column, and molecular exclusion on a protein pak glass 300 sw column. sodium dodecyl sulfate polyacrylamide gel electrophoresis (page) yielded two protein bands of approximately 14 kda and 22 kda. however, only one band was seen in native page. the mr of the lectin estimated by fast-performance li ... | 2003 | 14714732 |
| fusarium osteomyelitis of the foot in a patient with diabetes mellitus. | diabetic foot osteomyelitis is among the most common and serious complications in patients with diabetes mellitus. it is often a polymicrobial infection. we report the first case of foot osteomyelitis in a diabetic patient caused by fusarium solani. | 2003 | 14723375 |
| interactions of human phagocytes with moulds fusarium spp. and verticillium nigrescens possessing different pathogenicity. | fusarium spp. are emerging as important causes of invasive fungal infections. they tend to have decreased susceptibility to antifungal agents, making host defences very important. the ability of human phagocytes to cause damage to hyphae of fusarium solani, f. oxysporum and verticillium nigrescens, a mould with very low pathogenicity, was assessed using the 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]2h-tetrazolium-5-carboxanilide (xtt) metabolic assay. the oxidative burst, evidenced as superoxide ... | 2003 | 14725324 |
| effect of soil moisture on seed germination, plant growth and root rot severity of navy bean in fusarium solani infested soil. | the development of root rot and its severity were affected by numerous factors which through their interaction could distort results. in this study, a single factor, soil moisture was considered. using fusarium solani infested root rot soil we investigated the effect of three available soil moisture (asm) levels (i.e. 100%, 70% and 25%) on seed rot, germination, plant growth and root rot severity of navy bean in the greenhouse. percent germination differed strikingly averaging of 58.5, 89.5 and ... | 2003 | 15151296 |
| [disseminated fusarium solani infection in patients undergoing hematopoietic stem cell transplantation]. | a patient with a hematological malignancy is one example of a type of immunocompromised host, and critical opportunistic infections such as mycosis are not rare during medical treatment for such malignancy. candidiasis and aspergillosis are typical mycoses and their importance has been recognized widely and great progress attained in their prevention and medical treatment. however, allogenic hematopoietic stem cell transplantation (allo-hsct) to treat hematological malignancy has spread, and the ... | 2003 | 12913807 |
| fluorescence of the single tryptophan of cutinase: temperature and ph effect on protein conformation and dynamics. | the cutinase from fusarium solani pisi is an enzyme with a single l-tryptophan (trp) involved in a hydrogen bond with an alanine (ala) residue and located close to a cystine formed by a disulfide bridge between two cysteine (cys) residues. the cys strongly quenches the fluorescence of trp by both static and dynamic quenching mechanisms. the trp fluorescence intensity increases by about fourfold on protein melting because of the disruption of the ala-trp hydrogen bond that releases the trp from t ... | 2003 | 12929743 |