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[study of the antigenic structure of salmonella by means of immunoelectrophoresis].the method of immunoelectrophoretic analysis was applied to the study of the antigenic structure of 180 freshly-isolated and laboratory cultures of salmonellae belonging to 29 serological types. a complicated set of specific and common antigens of bacteria was revealed in direct and cross experiments with the use of homologous and heterogenous antisera; immunophoregrams were drawn for each of the serological type under study. general regularities in immunophoretic, diffuse and serological charac ...197555020
growth of staphylococcus and salmonella on frankfurters with and without sodium nitrite.conventional and nitrite-free frankfurters in loosely wrapped packages were compared as to their ability to support growth of salmonella, staphylococcus, and their naturally occurring spoilage flora at 7 c (simulating refrigerated storage) and 20 c (simulating possible temperature abuse). at 7 c salmonella did not grow in either type of frankfurter; staphylococcus and the natural spoilage flora sometimes grew more rapidly in the absence of nitrite, but the difference was not significant. at 20 ...1975952
effect of tilorone treatment on intracellular microbial infections in specific-pathogen-free mice.specific-pathogen-free cd-1 mice were treated orally with the drug tilorone (2,7-bis[2-diethylaminoethoxy]fluoren-9-one hydrochloride) at dosages of 10 or 100 mg per kg of body weight. drug was given 24 h before challenge and then every other day for up to 15 days. growth of sublethal doses of listeria monocytogenes, mycobacterium bovis (bcg montreal), m. tuberculosis h37rv, and salmonella enteritidis in the livers and spleens of intravenously challenged mice was significantly increased compared ...1975125066
effect of oral niridazole treatment on some bacterial infections in mice.treatment of specific-pathogen-free cd-1 mice with oral doses of 10 or 100 mg of niridazole per kg of body weight given 24 h before challenge and then every other day for up to 15 days altered the growth curves for listeria monocytogenes, mycobacterium bovis (bcg montreal), m. tuberculosis h37rv, and salmonella enteritidis seen in the livers and spleens of the treated animals. niridazole in an oral dosage of 10 mg/kg reduced (but did not eliminate) tuberculin hypersensitivity in the mycobacteria ...1975125067
interferon production in mice by the capsular polysaccharide of klebsiella pneumoniae.the capsular polysaccharide of klebsiella pneumoniae (cps-k) type 1, kasuya strain, induces interferon production in the blood of mice when injected intravenously. cps-k resembles bacterial endotoxin (lipopolysaccharide) in the time pattern of interferon production, with peak levels 2h after injection. cps-k on a weight basis exhibits a more potent interferon-inducing effect than lipopolysaccharide. the active substance responsible for the interferon-inducing activity of cps-k is the neutral cps ...1975166921
effect of klebsiella pneumoniae enterotoxin on intestinal transport in the rat.the effects on intestinal transport of either a semipurified preparation of enterotoxin elaborated by klebsiella pneumoniae or similaryly prepared control material were tested by marker perfusion studies in the small intestine of rats. at a concentration of 2 mg/ml, the enterotoxin produced net secretion of water, na, and cl in both jejunal and ileal segments; hco3 transport was not affected. net secretion was evident within 30 min after intorduction of the toxin and was maximal after 90 min. th ...1975169297
peyer's patch responsiveness to salmonella in mice. 1975234533
assay, characterization, and localization of an enterotoxin produced by salmonella.an enterotoxic factor isolated from cultures of salmonella yielded reproducible results in the suckling mouse model in contrast to other animal models. the enterotoxin appears to possess properties similar to both the heat-stable and heat-labile enterotoxins of escherichia coli. preliminary results indicate that the toxin is a protein, is located in the cell wall or outer-membrane fraction, and is difficult to separate from other cell wall constituents.1975234910
liver glutathione and glutathione reductase response of endotoxin-treated mice.the decrease in the level of liver glutathione (gsh) in endotoxin-treated mice was in part due to formation of glutathione disulfide (gssg). an electron-generating system (egs) had no effect when incubated with soluble liver extracts from normal controls but resulted in recovery of gsh amounting to 25% in endotoxin-treated animals. incubation in the absence of the egs caused a decline of 16% in the gsh in extracts from normal animals compared with a 50% decrease in endotoxin-treated animals. exc ...1975234913
biochemically aberrant salmonella enteritidis ser. newington from human sources in connecticut. 1975239492
[effects in the rat of a weak dose of acrolein inhaled continuously].the results of an experiment on 173 s. p. f. rats inhaling 0.55 ppm of acrolein, compared to 173 control rats, are reported. in what concerns the respiratory apparatus, this dose of acrolein affects its defense mechanisms, leading to a greater susceptibility to the airborne salmonella enteritidis infection, compared to the control group. these phenomena disappear spontaneously when intoxication is prolonged more than three weeks. on the contrary, the general toxic action, manifesting itself thro ...1975765136
[chemical and serological characterization of salmonella lipopolysaccharides from different phases of growth (author's transl)].chemical and serological investigations were carried out on lipopolysaccharides of 4 salmonella s-forms and of 1 sr-mutant, extracted from bacteria at different ages of culture (early exponential to stationary growth phase). the results show that the fatty acid composition of lipid a (lauric-, myristic-, palmitic-, and beta-hydroxy-myristic acids) does not undergo any significant change during the growth of the cultures. however, there are differences in the molar ratios of the fatty acids from ...1975766501
[some epidemiological and microbiological aspects of food poisoning due to salmonellae]. 1975775228
motility-indole-lysine medium for presumptive identification of enteric pathogens of enterobacteriaceae.detection of lysine decarboxylase activity is a useful supplement to reactions on triple sugar-iron (tsi) and urea agars in the initial examination of suspected pathogenic isolates from fecal cultures. owing to the added value of motility and indole production in the differentiation of enteric pathogens, we prepared and evaluated a motility-indole-lysine (mil) medium. the following 890 organisms were tested: 264 shigella, 2 edwardsiella, 182 salmonella enteritidis, 235 s. typhi, 3 arizona, 32 ye ...19751176632
[possibilities of differentiation of the specific positive reactions for pullorum disease].studied were sera obtained from three groups of birds experimentally infected with salmonella gallinarum-pullorum. salmonella enteritidis, and salmonella heidelberg, resp., using the whole serum agglutination reaction, the slow test-tube reaction (at 37 degrees and 50 degrees c), and the agar gel precipitation reaction as modified by aoki as well as the spot-test of williams. it was found that with the use of a typhus antigen it is not possible to differentiate the positive coagglutination react ...19751210001
the immunological basis of acquired cellular resistance in some infectious diseases caused by intracellular parasites. ii. antigen modulation produced on cells of animals infected with several species of intracellular parasites other than salmonella enteritidis. 19751234923
salmonella enteritidis infection in rats: antigens involved in cell-mediated immunity.when rats are sub-lethally infected with s. entertidis by the intravenous route, there is a period of at least 14 days in which peritoneal exudate cells are unable to migrate from capillary tubes on to glass surfaces; thereafter they migrate as do macrophages from normal animals. the migration of peritoneal cells from rats infected 18-21 days previously is inhibited by lipopolysaccharide (lps) prepared from homologous and heterologous strains of salmonella. the apparent non-specificity of lps ac ...1975779743
the immunological basis of acquired cellular resistance in some infectious diseases caused by intracellular parasites. i. antigenic modulation produced on some cells of mice infected with intracellular bacteria. 1975796545
[immunological investigation on refratory nature of salmonella infections]. 1975806640
nature of "memory" in t-cell-mediated antibacterial immunity: anamnestic production of mediator t cells.mice that survived an immunizing infection with listeria monocytogenes remained specifically resistant to lethal secondary infection for several months. this acquired, long-lived state of resistance was not dependent on activated macrophages that remained after the primary response. it depended, instead, on an acquired long-lived capacity on the part of immunized mice for generating mediator t cells faster and in larger numbers than normal mice. the number of mediator t cells generated in respon ...1975811558
pathogenesis of salmonella-associated arithritis in the rat.the distribution of joint lesions in rats with salmonella-associated arthritis (ssa), as determined in a detailed survey, resembles to a great extent the pattern of small joint involvement in human rheumatoid arthritis. such lesions, though regularly induced in the rat by the intravenous injection of live s.enteritidis, could not be evoked by the heat-killed organisms injected by various routes with and without extrinsic adjuvants. efforts to transfer saa from sensitized donors to either normal ...19751089597
blast transformation of rabbit b-derived lymphocytes by a mitogen extracted from nocardia.nocardia water soluble mitogen (nwsm) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine b-derived lymphocytes. in this paper, evidence is presented that nwsm is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti rtla serum) and with complement did not affect the strong incr ...19751090651
effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. i. depression of the afferent arc.bacterial lipopolysaccharide (lps), being a b cell mitogen, enhances antibody production. when given prior to an injection of sheep red blood cells (srbc) it caused a premature but not significantly increased production of antibody forming cells in regionally stimulated lymph nodes. there was a corresponding depression of t cell activity, as measured by the relative levels of delayed-type hypersensitivity (dth) in normal and lps-treated mice. although lps also induced hyperplasia of the reticulo ...19751090661
[salmonella infections in infants and small children hospitalized in the years 1968-1972]. 19751090982
the role of granulocytes in the activation of intravascular coagulation and the precipitation of soluble fibrin by endotoxin.this study examines the role of neutrophils (pmn) in the pathogenesis of endotoxin-induced microclot formation. it is intended to clarify whether granulocytes are involved in endotoxin-induced activation of intravascular coagulation (generation of soluble fibrin) and/or in endotoxin-induced precipitation of soluble fibrin. precipitation of soluble fibrin was achieved by injection of endotoxin into ancrod-infused rabbits with circulating soluble fibrin (first model). activation of intravascular c ...19751091310
plasma substitute induced impairment of the reticuloendothelial system function.currently used plasma substitutes are large colloids and are partly engulfed by reticuloendothelial (re) cells. this study was designed to investigate the magnitude and duration of the possible res dysfunction induced by plasma substitutes. the following substances were studied in both normal and burned (ld-10) cba mice: allogen albumin 3%, and commercial preparations of dextran 40, dextran 70, hydroxyethyl starch, gelatin and pvp. infusion volume was 20 ml/kg and i.v. infusion time 1 hour. anim ...19751092112
involvement of the upper respiratory tract in orally induced salmonellosis in mice.the possible involvement of the tissues of the head and neck during the early pathogenesis of orally or intragastrically induced murine salmonellosis was examined. viable counts of salmonella in the local lymphoid tissues and draining nodes indicated that the ileal peyer's patches were primarily involved in the development of a subsequent enteric fever, irrespective of the method of oral challenge. contamination of drinking water with a high concentration of salmonellae produced infection of the ...19751092770
an erythrocyte-sensitising antigen from vibrio cholerae.erythrocyte-sensitising antigens (ae) were prepared from vibrio cholerae serotypes, from el-tor vibrio, escherichia coli and salmonella enteritidis by digesting the organisms with naoh followed by precipitation with alcohol. when ae was used in indirect haemagglutination (iha) tests, the results in a number of cases were somewhat more sensitive and more specific than those obtained in classical agglutination tests. no cross reactions occurred between v. cholerae serotypes and e. coli and s. ente ...19751092860
[infection with "enteritis salmonella" at non-intestinal sites (author's transl)].so-called "enteritis salmonella" was isolated in five cases from extra-intestinal specimens (about 3% of all primary salmonella isolations in one year). the organisms were: s. typhimurium, s. enteritidis, s. java, s. panama, and s. infantis. in four of the five cases the clinical picture was not that of a salmonella infection. focal manifestations were a knee-joint empyema after long-term treatment with glucocorticoids, pleuritis in hodgkin's disease, osteomyelitis of the head of the tibia, and ...19751097228
endotoxin-sensitive membrane component of human platelets. 19751097934
[relation of the immune response in rats to the state of the lysosomes and intensity of protein synthesis].in stimulation of an immune response to salmonellae in rats by dibasol and methyluracil and its depression by chlorophos and an amine salt of 2,4-dichlorphenoacetic acid it was found that the intensity of the immune response depended more on the level of protein biosynthesis than on the state of lysosomes at the period preceding the immunization.19751098336
septic arthritis in sickle-cell thalassemia. pathophysiology of impaired response to infection.the knee of a boy with sickle-cell thalassemia became infected with salmonella enteritidis, sensitive to ampicillin. doses of ampicillin that achieved anti-bacterial titers of 32 in synovial fluid failed to eradicate the organism. the synovial fluid exhibited severe hypoxia, hypercapnia, and lactic acidosis. methods were developed to estimate the oxygen saturation of blood in regional venous capillaries, the degree of periarticular sickling, changes in the viscosity of local blood, and the artic ...19751098670
[resistance to antibiotics such as furazolidone and sulfathiazole of s. typhimurium and s. enteritidis strains isolated from humans and animals in 1960-1973]. 19751099366
specific antibodies and heterologous antigensin monofactor sera.a concentrated monofactor serum 9 (wellcome lot k 8028) precipitated against proteins from 2 different strains of salmonella enteritidis and from a strain of s. dublin, as well as against the proteinic moiety of the somatic antigen from s. typhi 0 901; no polysaccharide from the strains, sharing factor 9, precipitated against this serum. a concentrated monofactor serum 20 (wellcome lot k 6733) precipitated against the proteins from s. emek (8, 20), but not against its polysaccharide. a monofacto ...19751099401
postnatal development of pyrogenic sensitivity in guinea pigs.despite evidence of thermoregulatory ability from birth, neonates generally are unable to develop fever when challenged with endotoxin. this could be due to their small capacity for heat storage. to test this possibility, the pyrogenicity of s. enteritidis endotoxin (2 mug/kg, iv) was measured at both room (ta = 25 degrees c) and neutral (tn = 29-33 degrees c, depending on age) temperatures in 0- to 32-day-old unanesthetized guinea pigs, reared from birth at about 24 degrees c. control guinea pi ...19751100593
unusual enterobacteriaceae: a salmonella cubana that is urease positive.this is the first report of a naturally occurring salmonella that is urea positive. the strain was identified as salmonella cubana and it was typical in all biochemical, serological, and bacteriophage reactions, except that is produced urease strongly.19751100646
complement-mediated bactericidal system: evidence for a new pathway of complement action.the early components of human complement (c1, c4, and c2) plus certain serum euglobulins will kill pathogenic strains of shigella sonnei. serum from patients with hereditary c3 deficiencies and specific antiserums to c3, c5, and c6 were utilized to demonstrate the absence of requirements for late-acting complement components in this unusual bactericidal system.19751101380
[phage typing of strains of s. enteritidis isolated in bulgaria].studied were a total of 221 strains of salmonella enteritidis isolated from birds, pigs, cattle, dogs, rodents, forage, environment, food products of animal origin, and human beings, in terms of their phage typing, in order to study the epizootiology of the foci of infection. twelve phages were established: 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, 15, 19, 20 and 21. most widely distributed proved phage type 6a, followed by phage type 2a. the remaining phages occured more rarely, and some of them--15, 19, ...19751101519
profuse fimbriae conferring o-inagglutinability to several strains of s. typhi-murium and s. enteritidis isolated from pasta products. cultural, morphological, and serological experiments.during the last 2 years several o-inagglutinable salmonella strains were isolated from pasta products the typing of which presented great difficulties. serological and morphological tests (by electron microscope) revealed a fimbrial antigen which was thermolabile to a certain degree. this antigen represents (in corroboration of j.p. dugiud) a special type of fimbriae of salmonella and is morphologically different from type 1 and typ3-2 fimbriae previously described. it is probably an abnormal mu ...19751101574
[sensitivity to bacteriophage es18 of strains of "s. dublin", "s. enteritidis" and "s. blegdam" and related serotypes (author's transl)].sensitivity to bacteriophage es18 may be used as an additional test for identification of strains of some serotypes of salmonella. in the d group, the great majority (100/103) of the strains of s. enteritidis (h = g,m:-) and 7/7 strains of s. blegdam (h = g,m,q:-) are resistant. on the contrary, all the studied strains (111) of s. dublin (h = g,p:-) either vi+ or vi- are sensitive, as those of s. gallinarum-pullorum (61). two strains of s. kiel (possibly derivated from s. dublin) are sensitive a ...19751103676
reiter's disease in three boys.three cases of reiter's disease occurring in boys under the age of 16 are reported. one of these presented with a salmonella enteritidis diarrhoea. this conforms to the 'dysenteric' form of reiter's disease usually seen in europe and rarely reported in england. another presented with a monarticular arthritis of the knee, and the third has developed a chronic relapsing erosive arthritis as a result of sexually acquired reiter's disease--an occurrence not previously reported in this age group. we ...19751103755
antimicrobial resistance and r-factor transfer among isolates of salmonella in the northeastern united states: a comparison of human and animal isolates.the antimicrobial susceptibility of 718 isolates of salmonella from humans and of 688 isolates from animals was examined. of the 46 different serotypes among the isolates from humans, salmonella typhimurium accounted for 34%. thirty percent of isolates were resistant to one or more antibiotic(s). resistance to streptomycin was most common; resistance to tetracycline was next most common. over 50% of isolates of s. typhimurium and salmonella newport were resistant to four antibiotics. resistance ...19751104721
[case of plating out the causative agent of intestinal infections from the oral cavity]. 19751106082
[some problems of nomenclature and taxonomy of the enterobacteriaceae]. 19751106093
blastogenesis of human lymphocytes by endotoxin.lipopolysaccharides of s. typhimurium, s. enteritidis and e. coli at wide range of concentrations were used to induce blastogenesis in human and mouse (nude) lymphocytes. human lymphocytes from peripheral blood showed positive response to at least one source of lps (stimulation index of 2-9). the optimum concentration resulting in maximum stimulation varied with different individual, sources and concentrations of lps used. lymphocytes from cord blood failed to respond to lps, but had positive re ...19751107213
[role of the mammary gland in the development of immunity with the intracisternal administration of antigen]. 19751108387
[study of salmonella cultures. iv. immunogenicity of salmonella resistant to antibiotics].three avirulent and 5 slightly virulent for birds mutants of salmonella enteritidis and salmonella braenderup, resistant to streptomycin (s), chlorteracyclin (ct), and chloramphenicol (ch) were used in the production of live vaccines. the latter were tested for immunogenicity on albino mice and birds. on the eighth and 15th day following vaccination the protein fractions in the blood serum of the birds were followed up through electrophoresis on agar gel, while the agglutinin titer was determine ...19751108410
canine salmonellosis: a review and report of dog to child transmission of salmonella enteritidis.dogs have been shown to harbor 53 salmonellae serotypes. multiple simultaneous infections with 2 to 4 serotypes have been observed. the prevalence of canine salmonellosis may be a high as 27 per cent. salmonella typhimurium and s. anatum are the most common etiologic agents. dogs commonly experience a sub-clinical course of salmonellosis. some investigators state that the dog may serve as a source of human infections. a few reports in the literature have documented this fact. the transmissions ...19761108681
[enteritis-salmonellae as a cause of unusual infection courses]. 1976819821
survival of bacteria in seawater using a diffusion chamber apparatus in situ.a microbiological survival chamber for in situ environmental studies involving microorganisms of public health significance was developed. the autoclavable chamber was provided with a supportive base for proper flow orientation and a battery-powered stirring mechanism for continuous internal agitation. the performance of the chamber and its ancillary units was evaluated in estuarine waters by diffusion studies and survival tests with eight species of bacteria isolated from environmental sources. ...1976820259
susceptibility of clinically important gram-negative bacilli to 15 antibiotics. comparison of patterns in poland and federal republic of germany.three hundred and sixty strains of gram-negative bacilli (escherichia coli, salmonella enteritidis, enterobacter cloacae, providencia, indole-positive and indole-negative proteus, klebsiella pneumoniae, pseudomonas aeruginosa and serratia marcescens), were isolated by random in poland and germany, and tested by agar dilution method for susceptibility to 15 antimicrobial agents, including six cephalosporin derivatives, colistin, gentamycin and tobramycin, three newer tetracyclines, ampicillin and ...1976824896
[immunological efficacy of live streptomycin-dependent salmonellae vaccines used orally in experiment on monkeys].experiments were conducted on 99 monkeys; a study was made of the reactogenic properties and the immunological efficacy of the streptomycin-dependent s. enteritidis and s. typhimurium mutants in oral application. the results of clinical, bacteriological, pathomorphological and fluorescent-serological studies demonstrated that the vaccines in the doses under study were areactogenic for monkeys in 5-fold oral administration and promoted development of marked immunity. the greatest immunizing effec ...1976827161
ribonucleic acid synthesis in normal and immune macrophages after antigenic stimulus.macrophage ribonucleic acid (rna) synthesis is an important metabolic process intimately related to the function of these cells. mouse peritoneal macrophage rna was extracted with phenol in the presence of bentonite and electrophoresed on composite agarose-polyacrylamide gels. the pulse-chase technique was used to follow the precursor relationships in macrophage ribosomal rna (rrna) maturation. the rrna species at 18s and 28s appeared at 15 and 45 min, respectively, after rna synthesis was halte ...1976971940
[study of an explosive outbrake of gastroenteritis by salmonella enteritidis, infantis serotype]. 19761029851
[experience in controlling the salmonella carrier state on a pedigree poultry breeding farm].systematic microbiologic control was carried out in the 1972-1975 period on an elite poultry farm whereas from the 23,724 samples studied, taken from objects of the epizootic chain forage-birds-hatchery, 78 cultures of salmonella organisms of 14 species or 0.32 per cent of the total number of samples were isolated. a trend was observed toward the year-to-year drop in the number of positive findings of salmonellae, and by the end of 1975 the results were negative. this fact was backed by the inve ...19761030870
[salmonella bacilli in children hospitalized in the years 1956-1974]. 1976796892
[experimental study of the stability of avirulent properties and the immunogenicity of suppressor revertants of streptomycin-dependent salmonella mutants].the authors aimed at the experimental confirmation of avirulent properties of suppressor revertants of streptomycin-dependent salmonella mutants and also at the study of their immunogenicity. stability of the loss of virulence by the suppressor revertants was established by studying the virulence of the subcultures isolated from them, differing by the genotype. repeated passage of suppressor revertants of s. typhimurium rev8, s. enteritidis rev5, s. pullorum rev through the biological objects hi ...1976797191
resistance of mice to krebs ascites tumour, sarcoma s180 and pc6 plasmacytoma after immunisation with salmonella enteritidis 11rx.resistance to krebs ascites tumour, sarcoma s180 (ascitic form) and pc6 plasmacytoma can be induced with prior immunisation with live salmonella enteritidis 11rx. one thousand to ten thousand more tumour cells are needed to give an ld50 after immunisation. resistance to pc6 could be recalled 137 days after intravenous immunisation with 11rx protein antigen intraperitoneally as measured by prolonged survival or monitoring the death of 5[131i]-iodo-2'-deoxyuridine labelled tumour cells in vivo. th ...1976797376
local and systemic effects in the non-specific tumour resistance induced by attenuated salmonella enteritidis 11rx in mice.suppression of growth of a number of different murine tumours, injected subcutaneously or into the foot pad, was induced by prior intraperitoneal or intravenous immunisation with salmonella enteritidis 11rx. the effect of immunisation on tumour growth was relatively minor, but could be enhanced by addition of 11rx antigen preparations to the suspensions of tumour cells used for challenge. the significance of these findings is discussed.1976797377
a role for antibody in the expression of cellular immunity to salmonella typhimurium c5.immunisation of mice with alcohol-killed salmonella typhimurium c5 vaccine before infection with salmonella enteritidis 11rx enhanced the ability of these mice to control and eliminate a challenge dose of salmonella typhimurium c5, compared to mice which only received live salmonella enteritidis 11rx organisms. when alcohol-killed salmonella enteritidis se795 vaccine was used instead of the salmonella typhimurium c5 vaccine, this effect was not observed. these observations imply that both humora ...1976797378
the role of thymus-derived cells in immunity to salmonella infection.the ability of thymus-derived (t) cell depleted mice to eliminate a dose of the normally avirulent 11rx strain of salmonella enteritidis was compared to that of normal mice by following the fate of the challenge organisms in the liver and spleen of both types of mice. although the mice did not require normal numbers of t cells to survive infection with salmonella enteritidis 11rx, t cell depletion reduced the ability of mice effectively to eliminate the organism from their tissues. in addition, ...1976797379
leukocyte inhibiting factor (lif) production from human lymphocytes stimulated by bacterial lipopolysaccharides.leukocyte inhibiting factor (lif) release from lymphocytes cultures stimulated by bacterial lipopolysaccharides (lps) has been investigated. human lymphocytes cultured in the presence of e, coli or s. enteritidis lps were able to release in the supernatants a substance with the following properties: a) same elution pattern of bovine serum albumin (mw 69000) when filtered on sephadex g-100; b) it migrates on disc electrophoresis a an albumin; c) not dialyzable; d) not cytotoxic; e) inhibition of ...1976798596
scanning electron microscopic studies on the microstructure of s and r type colonies of salmonella and shigella groups. 1976802350
adhesive properties of vibrio cholerae: nature of the interaction with intact mucosal surfaces.two companion papers in this series have characterized the interaction between vibrio cholerae and the surfaces of eukaryotic cells. the present paper reports studies of the association between vibrios or salmonella enteritidis and intact slices of intestinal tissue. a significant number of differences were noted in the characteristics of bacterial adhesion in these systems. the results are interpreted to indicate the presence of at least two receptors for vibrio adhesion on the mucosal surface ...1976780274
[resistance of the salmonella used for deratization to some environmental factors]. 1976783016
[influence of mutation in the gene-suppressor su-str on the virulence of salmonella enteritidis].virulent and avirulent cells resistant to streptomycin were revealed in the population of avirulent streptomycin-resistant mutant (obtained from the supressor revertant str-d s. enteritidis mutant) in seeding on a medium with streptomycin. transduction analysis of the isolated avirulent and virulent strains demonstrated that mutation in the gene-suppressor su-str+ led to the loss of virulence. in connection with the presence in the genom of suppressor revertants of the streptomycin-dependent mut ...1976785903
bioassay of endotoxin clearance in vivo and by perfused rat liver.endotoxin clearances in vivo and by the isolated perfused rat liver were evaluated via bioassay in lead-sensitized rats. a linear relationship between the probit of shock lethality and the endotoxin dose in the probit range of 4-6 was validated. endotoxin clearance in normal, fed rats displayed a linear relationship between the logarithm of the blood endotoxin concentration and time throughout the period of 15-240 min at doses of 500 and 1,000 mug/ rat; the half-time values were 58-63 min. decre ...1976786043
hypoglycemic depression of res function.the intravascular removal rates of colloidal carbon and of biologically active endotoxin by the reticuloendothelial system (res) were evaluated as a function of blood-glucose levels. there was a significant negative correlation of carbon clearance half time on blood glucose in both saline-treated and insulin-treated rats. insulin hypoglycemia depressed res carbon clearance with the maximal effect occurring at blood glucose values below 30 mg/dl. insulin hypoglycemia also severely impaired the in ...1976786044
salmonella food poisoning from turkeys: a combined medical veterinary investigation. 1976786953
adjuvant action of capsular polysaccharide of klebsiella pneumoniae on antibody response. v. further biological properties of the active substance.the neutral fraction (neutral cps-k) of klebsiella pneumoniae capsular polysaccharide (cps-k) from type 1, kasuya strain, has already been reported as the active substance responsible for the strong adjuvant effect of cps-k. the present results demonstrate that neutral cps-k exhibits further common biological activities with lipopolysaccharide (lps) isolated from salmonella enteritidis. the intensity of the lethality in mice of neutral cps-k by the intraperitoneal route is very similar to that o ...1976787603
phage typing system for salmonella enteritidis.a system is described for the phage typing of salmonella enteritidis. the system was developed using a number of bacteriophages that were isolated from sewage.1976788636
[induced filament formation in s. enteritidis by means of different sodium and chloride salts and different carbohydrates].we have previously observed filament formation in s. enteritidis which grew on media containing different salts and carbohydrates. in this study, sodium citrate, sod. carbonate, sod. molybdate, sod. acetate, sod. sulfate, potassium chloride, calcium chloride, saccharose and glucose were used as inducing agents. different concentrations of most of the above substances were effective in filament formation in s. enteritidis. temperature (37 degrees c and room temperature), ph of media and state of ...1976790103
[comparison of various aspects of normal and filament forms of s. enteritidis iv].induced filament formation of s. enteritidis by means of sodium chloride was previously reported. in this study various aspects of normal and filament forms of s. enteritidis are compared. filament forms had lost some of their biochemical activities when compared with the normal forms of s. enteritidis. there was no serological difference between the two forms. we were unable to show any difference in antibiotic sensitivity, because concentrated sodium chloride inhibited cell growth. no differen ...1976790106
[post-salmonellosis rheumatism].reports of forty-six cases of aseptic polyarthritis following intestinal salmonlla infections have been published. the organism involved is always a "minor" salmonella either typhimurium or enteritidis. the interval separating the diarrhoea from the joint manifestations is usually one or two weeks. post-salmonellosis rheumatism is most frequently polyarthritis involving the large joints accompanied by fever that is sometimes high. the sedimentation rate is increased and hyperleucytosis and discr ...1976790532
effect of capsular polysaccharide of klebsiella pneumoniae on host resistance to bacterial infections. ii. effects on peritoneal leukocytes of normal mice and mice infected with virulent salmonella enteritidis.in normal mice, the total count of peritoneal leukocytes was markedly decreased after intraperitoneal (i.p.) injection of the capsular polysaccharide of klebsiella pneumoniae (cps-k) depending on the dosage injected. this decrease was mainly due to the depletion of macrophages, and a decrease in the number of lymphocytes occurred to a lesser extent. cps-k in relatively smaller doses mobilized polymorphonuclear neutrophilic leukocytes (pmn) into the peritoneal fluid but it decreased them transien ...1976792533
ineffectiveness of lipopolysaccharide for preventing the tolerance induction in bone marrow-derived lymphoid cells with dinitrophenyl-poly-l-(glutamic acid, lysine).the effect of endotoxin or lipopolysaccharide (lps) on tolerance induction in bone marrow-derived lymphoid cells (b cells) was investigated. dinitrophenylated amino acid copolymer-l-(glutamic acid, lysine) (dnp-gl) acts as a potent tolerogen on normal and dnp-primed b cells. lps significantly enhanced the anti-sheep red blood cell plaque-forming cell (anti-srbc pfc) response that occurred after the immunization with a low dose of srbc. lps did not induce the primary anti-dnp pfc response after t ...1976792534
agglutination with o antisera of salmonella exposed to antibiotics.five strains of salmonella were grown on agar containing subinhibitory concentrations of ampicillin, gentamicin, tetracycline, or chloramphenicol, and on drug-free agar (control). antigens were prepared by heating bacteria suspensions in saline solution at 100 c or by suspension in alcohol. antigens were tested for agglutinability with somatic o antisera and fluorescent antibody staining. the alcohol-treated antigens prepared from organisms grown in the presence of antibiotics other than ampicil ...1976793377
microbial formation of nitrosopyrrolidine from nitrosoproline.the fate of orally administered npro was determined in rats. unchanged npro could be recovered in the urine, while npy was detected in the faeces of two of three rats tested. after successive administration of npro to the same rats, their intestinal contents were inoculated into media containing npro to test for npy formation. decarboxylation of npro occurred in the faeces cultures from those rats whose faeces had contained npy. when the faeces from 10 mice were inoculated into the npro media, a ...1976793983
[survival of salmonellas used for harmful rodent control in the environment]. 1976794648
induction of generalized shwartzman reaction(gsr) in irradiated rabbits by a single injection of endotoxin. 1976775679
salmonella in horses: a source of contamination of horsemeat in a packing plant under federal inspection.cecal samples from 270 slaughter horses revealed that 41 samples (15.1%) contained salmonella. of 233 horsemeat samples tested, salmonella was isolated from 62 samples, or 26.6%. only 2 of 158 human stool specimens from the plant workers revealed salmonella. predominant serotypes isolated from the horsemeat were salmonella enteritidis good and anatum, whereas the serotypes agona and derby predominated the horse cecal isolates. preliminary data indicate that the high percentage of meat contaminat ...1976776084
role of host factors in the pathogenesis of salmonella-associated arthritis in rats.to evaluate the roles of the infectious agent and the host in the pathogensis of salmonella-associated arthritis (saa), 1o(3) to 10(6) heat-killed salmonella enteritidis were injected directly into involved carpal joints in normal and actively immunized rats and in hosts adoptively immunized by the intravenous transfer of spleen cells from syngeneic donors with ongoing saa. as many as 10(6) living salmonellae invariably failed to generate more than a transient inflammatory response in normal rat ...1976776830
[induced filament formation in salmonella enteritidis by means of sodium chloride].induced morfological variations of bacteria by means of different agents has been known for many years. in this study the influence of sodium chloride on filament formation of s. enteritidis is studied, and the concentrations between 5-7% has been found effective in filament formation.1976778550
transformation of escherichia coli by r404 factor dna and properties of the transformants.three r-determinants (cb cm cf) out of eight present in r404 factor were transferred in transformation of escherichia coli by the plasmid dna isolated from minicells. the r-determinants acquired in this way formed the replicon tr404-1 and were untransferable by conjugation unless another self-transmissible plasmid was present in recipient. the evidences are presented in support of hypothesis that the transfer events consist in infection of transformant cells with sex factor, recombination betwee ...1976779405
febrile illnesses resulting in hospital admission: a bacteriological and serological study in jakarta, indonesia.a study of 741 indonesian patients with fever was carried out in order to determine what serious febrile illnesses are prevalent in jakarta. all patients were hospitalized primarily because of fever and were studied by bacteriological and serological methods. bacteremia due to salmonella typhi (150 cases), s. enteritidis (36 cases), or both (2 cases) was common in both children and adults. one s. enteritidis isolate was chloramphenicol resistant. serological evidence of salmonella infection was ...1976769571
failure of endotoxin to increase nonspecific resistance to infection of lipopolysaccharide low-responder mice.in vitro and in vivo responses to lipopolysaccharide (lps) and various other bacterial immunostimulants were compared in c3h/he low-responder mice. the principal findings were as follows. (i) their splenic lymphocytes were stimulated by various gram-negative mitogens such as an escherichia coli peptidoglycan, a detoxified derivative of lps, and even endotoxins extracted by trichloroacetic acid that are known to contain protein; spleen cells of these mice were also transformed by two other b-cell ...1976773822
immunosuppressive effect of bacterial lipopolysaccharide on antibody response.injection of endotoxins (bacterial lipopolysaccharide: lps) several days prior to immunization causes the suppression of antibody response. the supressive effects of several kinds of lps preparations on the plaque-forming cell (pfc) antibody response in the spleen of mice were examined after immunization with sheep red blood cells (srbc). glycolipids obtained from heptoseless mutants(reform) of salmonella or its lipid a preparation coupled artificially with bovine serum albumin (bsa) are capable ...1976775151
fever: exchange of shivering by nonshivering pyrogenesis in cold-acclimated guinea pigs.the pyrogenic response of adult, unanesthetized guinea pigs to 2 mug/kg iv of salmonella enteritidis endotoxin was measured at 27 and 7 degrees c ambient temperatures, both before and after an 8-wk exposure to 7 degrees c. there were no significant differences between the onset, maximum height, and total duration of the fevers produced before and after cold acclimation in both thermal environments. however, in 27 degrees c, before cold acclimation, fever production was associated with vigorous s ...1976765315
effect of propranolol on endotoxin-induced pyrogenesis in newborn and adult guinea pigs.previous studied have shown that nonshivering thermogenesis (nst) is the prevailing mechanism of fever production in both newborn (during the first 2-3 wk of life) and adult cold-acclimated guinea pigs. this study was undertaken to determine whether this process may be mediated by noradrenergic sympathetics. the temperatures in the interscapular brown fat pad (tbat) and the colon (tre), the rate of oxygen consumption (vo2), and shivering activity were measured continuously for 5 h at 27 degrees ...1976765316
endotoxin toxicity in rats is enhanced by tilorone.the relatively nontoxic drug, tilorone, greatly enhanced the susceptibility of rats to lethal effects of endotoxin. the magnitude of the synergy was similar to that produced by adrenalectomy, but the effect of tilorone was not mediated by the adrenal glands. the histopathologic effects of endotoxin plus tilorone resembled those produced by much larger doses of endotoxin alone, including instances of the generalized shwartzman reaction.1976766017
adjuvant disease induced by mycobacteria, determinants of arthritogenicity.genetic, endocrine and immunological factors are probably involved in adjuvant polyarthritis. the nature of the vehicle and of the mycobacterial components administered also has a major influence. it was originally assumed that arthritogenicity and adjuvanticity of mycobacterial fractions such as wax d were intimately related. our previous findings showed that the water soluble adjuvant (wsa) of m.smegmatis which could substitute for mycobacterial cells in freund's complete adjuvant and induce d ...1976181972
non-specific immunity after local immunization.the most important advantage of local immunization is the non-specific effect. the active protection test in mice shows an immunity against oral infection with virulent salmonella typhimurium bacteria after oral immunization with heterologous inactivated enterobacteria. we observed the same non-specific protection in a viral model. after oral immunization with inactivated s. typhimurium bacteria, the mice are protected against oral infection with a virulent parapoliomyelitis virus. in the phagoc ...1976182583
starvation survival of salmonella enteritidis.washed cells of salmonella enteritidis harvested from a defined medium during logarithmic growth were subjected to starvation in ph 7 phosphate buffer at 37 c. viability was measured by slide cultures and plate counts. the survival of cell suspensions equivalent to 1 to 10 mg (dry wt)/ml was influenced by cryptic growth. the rate of cryptic growth, assessed by plate counts, increased with cell density and could not be alleviated by starvation with dialysis. dialysis of the starving culture did r ...19761369
effect of capsular polysaccharide of klebsiella pneumoniae on host resistance to bacterial infections. i. induction of increased susceptibility to infections in mice.when klebsiella pneumoniae capsular polysaccharide (cps-k) from type 1, kasuya strain, was injected intraperitoneally (i.p.) immediately before i.p. bacterial challenge, the survival time of mice infected with salmonella enteritidis nub 1 (virulent strain) was shortened and the mortality rate for mice infected with s. enteritidis nub 31 (avirulent strain) was enhanced. the promotion of infection with s. enteritidis nub 1 by cps-k depended upon its dose, the effect of cps-k being demonstrable up ...19769528
effect of sera from immunologically altered mice on phytohemagglutinin-induced mouse lymphocyte transformation.the dna-synthetic response of mouse spleen cells to phytohemagglutinin in vitro is depressed by the addition of normal mouse serum. the effects of sera from mice that had been modified immunologically were examined. increased depressive activity was found in sera from nude mice and from mice recently treated with anti-thymocyte globulin, but no change was found in sera from thymectomized, irradiated, bone marrow-reconstituted mice, nor from lethally or sublethally irradiated or cyclophosphamide- ...197613697
letter: colonic involvement in salmonellosis. 197659131
cell wall lipopolysaccharide response to the colib plasmid mutants.mutants of colib plasmid affected the synthesis of o-side chains of lipopolysaccharides (lps) in salmonella. the plasmid srd 25 (defective in colicin synthesis) caused a significant decline of rhamnose and mannose content and lack of abequose in lps of s. typhimurium. the number of repeating units in o-side chains was decreased after the indroduction of srd 25. cultures of s. typhimurium and s. enteritidis harboring drd2 (derepressed in colicin production) polymerised dideoxyhexose-defective o-s ...197659522
salmonella gallinarum - salmonella enteritidis relationship in rabbits.the polysaccharide of s. gallinarum, sharing factors 9.12 with the salmonellae of group d. cross-reacts with one of two serological determinants, present in the polysaccharide of s. enteritidis, and is different from the determinant responsible for the cross-reaction of s. enteritidis and s. typhy polysaccharides. the absorption of anti s. gallinarum sera with proteins from s. enteritidis removed all the antibodies induced for the homologous antigens; in contrast, the absorption of the anti s. e ...197766825
[cellular immunity in experimental salmonellosis and transfer agent of immunity (author's transl)]. 197767614
outbreaks of salmonella food-poisoning over a period of eight years from a common source.in an outbreak of turkey-borne food-poisoning lasting 8 years there were 185 cases and 1 death. 10 incidents were traced to one breeding establishment. the investigation of the outbreak revealed the advantage of close medicoveterinary collaboration.197768344
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