Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cell culture of the rice brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae). | the rice brown planthopper (bph), nilaparvata lugens (stål), is one of the most destructive insect pests of rice in asia. although resistant rice varieties can be effective in managing planthopper populations, bph has repeatedly been able to overcome resistant rice varieties. it is possible that bph adaptation to resistant rice varieties may be related to its endosymbionts. we investigated methods for culturing bph cells in order to study in-depth interactions between rice, bph, and its endosymb ... | 2014 | 24399256 |
| bioefficacy of piper ribesioides (piperaceae) extracts against nilaparvata lugens stal. (homoptera: delphacidae). | the extracts of stem and seeds of piper ribesioides (piperaceae) were prepared in hexane, dichloromethane, ethyl acetate and ethanol and evaluated for their efficacy against 3-day-old adult brown plant hoppers, nilaparvata lugens stal under laboratory conditions. the treatment was given topically and results suggest the dose dependent response related to exposure time. the ethyl acetate extract was the best with an lc50 = 738 ppm-, 24 h post-treatment. there was substantial increase in activity ... | 2014 | 26084101 |
| actin-mediated transovarial transmission of a yeastlike symbiont in the brown planthopper. | the brown planthopper nilaparvata lugens (hemiptera, delphacidae) harbors an obligate endosymbiont called the yeastlike symbiont (yls) in their abdominal fat body. yls, a filamentous ascomycete belonging to the family clavicipitaceae, does not spend any part of its life cycle outside the planthopper's body. the yls is transferred to the next generation via transovarial transmission; it enters the epithelial plug at the posterior end of the host female's ovariole and is transferred to her offspri ... | 2014 | 24315960 |
| decrease of insecticide resistance over generations without exposure to insecticides in nilaparvata lugens (hemipteran: delphacidae). | the brown planthopper, nilaparvata lugens (stål), is one of the most important insect pests on paddy rice in tropical and temperate asia. overuse and misuse of insecticides have resulted in the development of high resistance to many different insecticides in this pest. studies were conducted to evaluate the change of resistance level to four insecticides over 15 generations without any exposure to insecticides in brown planthopper. after 15 generations' rearing without exposure to insecticide, b ... | 2014 | 25195455 |
| comparisons of topical and spray applications of two pesticides, triazophos and jinggangmycin, on the protein content in the ovaries and fat bodies of the brown planthopper nilaparvata lugens stål (hemiptera:delphacidae). | the pesticide-induced stimulation of reproduction in pests is one of the most important mechanisms of pest resurgence. there have been numerous reports on the insecticide-induced stimulation of reproduction. however, the relationship between pesticide application method and pest resurgence (stimulation of reproduction) has received little attention. here, we studied the effect of two treatment methods, triazophos (tzp) and jinggangmycin (jgm), on the protein content in the ovaries and fat bodies ... | 2014 | 25175656 |
| isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (sogatella furcifera). | the whitebacked planthopper sogatella furcifera (horváth) (hemiptera: delphacidae) is one of the most harmful pests of rice. in this study, 18 polymorphic microsatellite markers were developed from s. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing repeat protocols. microsatellite polymorphism was investigated using 32 individuals from one natural population. these 18 simple sequence repeat markers showed a number of alleles ... | 2014 | 25158251 |
| identification of promoter polymorphisms in the cytochrome p450 cyp6ay1 linked with insecticide resistance in the brown planthopper, nilaparvata lugens. | imidacloprid resistance in the brown planthopper, nilaparvata lugens, is primarily the result of the over-expression of cytochrome p450 monooxygenases. here, a field-collected strain of n. lugens was shown to be highly resistant to both imidacloprid and buprofezin. insecticide exposure and quantitative real-time pcr revealed that its resistance was mainly associated with a cytochrome p450 gene, cyp6ay1. cyp6ay1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. in the 5 ... | 2014 | 25124988 |
| physiological efects of paichongding applied to rice on the nilaparvata lugens (stå l), the brown planthopper. | nilaparvata lugens (stål) is a major rice pest in asia. paichongding is a novel neonicotinoid insecticide developed in 2008. the effects of this insecticide on the activity of detoxification enzymes of n. lugens and on rice resistance to the pest were examined in the laboratory. the results showed that paichongding could significantly decrease the acetylcholinesterase and gshs transferase activities of n. lugens. the variation tendency of mixed function oxidase (mfo) activity was similar with th ... | 2014 | 25042427 |
| chitin deacetylase family genes in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | chitin deacetylases (cdas) are enzymes required for one of the pathways of chitin degradation, in which chitosan is produced by the deacetylation of chitin. bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding cdas in nilaparvata lugens (nlcdas). phylogenetic analysis showed that insect cdas were clustered into five major groups. group i, iii and iv cdas are found in all insect species, whereas the pupa-specific group ii and gut-specific group v c ... | 2014 | 24989071 |
| genetic mapping of the rice resistance-breaking gene of the brown planthopper nilaparvata lugens. | host plant resistance has been widely used for controlling the major rice pest brown planthopper (bph, nilaparvata lugens). however, adaptation of the wild bph population to resistance limits the effective use of resistant rice varieties. quantitative trait locus (qtl) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene bph1. qtl analysis in iso-female bph lines with single-nucleotide polymorphism (snp) markers dete ... | 2014 | 24870048 |
| heat-dependent fecundity enhancement observed in nilaparvata lugens (hemiptera: delphacidae) after treatment with triazophos. | the brown planthopper nilaparvata lugens (stål) (hemiptera: delphacidae) is a serious pest of rice crops in the temperate and tropical regions of asia and australia, and it is also a classic example of an insecticide-induced resurgent pest. brown planthopper outbreaks have been reported to be closely associated with pesticide application. previous studies have shown that the insecticide triazophos enhances thermal tolerance and fecundity in brown planthopper. however, the effects of triazophos a ... | 2014 | 24763100 |
| the content of amino acids in artificial diet influences the development and reproduction of brown planthopper, nilaparvata lugens (stål). | nitrogen availability from dietary protein has profound effects on the physiology and ecology of insect herbivores. the amount of amino acids consumed by nilaparvata lugens impacts its phenotypic characteristics and reproduction. in this work, we hypothesized that amino acids deficiency leads to physiological trade-offs between survival and reproduction. we investigated the effect of larval nutrition on larval period, wing dimorphism, egg production, ovarian development, lifespan, and stored nut ... | 2014 | 24753101 |
| heavy metal bioaccumulation and mobility from rice plants to nilaparvata lugens (homoptera: delphacidae) in china. | samples of soils, rice plants, and the adult, long-winged, brown planthoppers, nilaparvata lugens (stål) (homoptera: delphacidae), were collected from 18 sites of 9 regions in southern china. the concentrations of seven elements (cu, zn, as, mo, ag, cd, and pb) were measured using inductively coupled plasma mass spectrometry. heavy metal mobility and bioaccumulation were analyzed in the rice plant-n. lugens system. the concentrations of zn, as, cd, and pb in rice plants were positively correlate ... | 2014 | 24735989 |
| will climate change affect outbreak patterns of planthoppers in bangladesh? | recently, planthoppers outbreaks have intensified across asia resulting in heavy rice yield losses. the problem has been widely reported as being induced by insecticides while other factors such as global warming that could be potential drivers have been neglected. here, we speculate that global warming may increase outbreak risk of brown planthopper (nilaparvata lugens stål.). we present data that demonstrate the relationship between climate variables (air temperature and precipitation) and the ... | 2014 | 24618677 |
| outbreaks of the brown planthopper nilaparvata lugens (stål) in the yangtze river delta: immigration or local reproduction? | an effective control strategy for migratory pests is difficult to implement because the cause of infestation (i.e., immigration or local reproduction) is often not established. in particular, the outbreak mechanisms of the brown planthopper, nilaparvata lugens (stål), an insect causing massive losses in rice fields in the yangtze river delta in china, are frequently unclear. field surveys of n. lugens were performed in jiangsu and zhejiang provinces in 2008 to 2010 and related historical data fr ... | 2014 | 24558459 |
| [ecological responses of brown planthopper, nilaparvata lugens, to triazophos and deltamethrin on transgenic bt rice lines and their non-bt parental rice]. | in this study, the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae), cultured on bt rice lines (t1c-19 with cry1c gene and t2a-1 with cry2a gene) and their parental rice mh63, were treated with a series of concentrations of triazophos and deltamethrin when they developed up to the third instar for the evaluation of the effects of bt rice on bph resurgence induced by pesticides. the results indicated that bph nymphal duration decreased with the increasing concentration of delt ... | 2014 | 25796912 |
| different expression profiles suggest functional differentiation among chemosensory proteins in nilaparvata lugens (hemiptera: delphacidae). | chemosensory proteins (csps) play various roles in insect physiology including olfaction and development. the brown planthopper, nilaparvata lugens stål, is one of the most notorious rice pests worldwide. the wing-from variation and annually long distance migration imply that olfaction would play a key role in n. lugens behavior. in this study, full-length cdnas of nine csps were cloned by the rapid amplification of cdna ends procedure, and their expression profiles were determined by the quanti ... | 2014 | 25527582 |
| the insect ecdysone receptor is a good potential target for rnai-based pest control. | rna interference (rnai) has great potential for use in insect pest control. however, some significant challenges must be overcome before rnai-based pest control can become a reality. one challenge is the proper selection of a good target gene for rnai. here, we report that the insect ecdysone receptor (ecr) is a good potential target for rnai-based pest control in the brown planthopper nilaparvata lugens, a serious insect pest of rice plants. we demonstrated that the use of a 360 bp fragment (nl ... | 2014 | 25516715 |
| effects of exogenous plant growth regulator abscisic acid-induced resistance in rice on the expression of vitellogenin mrna in nilaparvata lugens (hemiptera: delphacidae) adult females. | recent study showed that exogenous abscisic acid (aba) acts as a regulator of plant resistance. this study investigated average injury scale and callose contents of rice, and vitellogenin (nlvg) mrna expression in nilaparvata lugens (stål) (hemiptera: delphacidae) adult females after third instar nymphs fed on exogenous aba-treated susceptible [taichung native one (tn1)] and moderately resistant (ir42) rice cultivars. the results showed that exogenous aba significantly decreased average injury s ... | 2014 | 25502025 |
| estimation of the age and amount of brown rice plant hoppers based on bionic electronic nose use. | the brown rice plant hopper (brph), nilaparvata lugens (stal), is one of the most important insect pests affecting rice and causes serious damage to the yield and quality of rice plants in asia. this study used bionic electronic nose technology to sample brph volatiles, which vary in age and amount. principal component analysis (pca), linear discrimination analysis (lda), probabilistic neural network (pnn), bp neural network (bpnn) and loading analysis (loadings) techniques were used to analyze ... | 2014 | 25268913 |
| wingless gene cloning and its role in manipulating the wing dimorphism in the white-backed planthopper, sogatella furcifera. | wingless gene (wg) plays a fundamental role in regulating the segment polarity and wing imaginal discs of insects. the rice planthoppers have an obvious wing dimorphism, and the long- and short-winged forms exist normally in natural populations. however, the molecular characteristics and functions of wg in rice planthoppers are poorly understood, and the relationship between expression level of wg and wing dimorphism has not been clarified. | 2014 | 25266639 |
| radar observations of the seasonal migration of brown planthopper (nilaparvata lugens stål) in southern china. | the summer and autumn migrations of the brown planthopper (nilaparvata lugens) were observed in southern china with a millimetric scanning entomological radar and a searchlight trap supplemented with capture in field cages, field surveys, and dissections of females. nilaparvata lugens took off at dusk and dawn in summer, but in autumn there was sometimes only a dusk take-off. the variation of the area density of the radar targets indicated that flight durations were about 9-10 h. in summer, plan ... | 2014 | 25229712 |
| insecticide susceptibilities in populations of two rice planthoppers, nilaparvata lugens and sogatella furcifera, immigrating into japan in the period 2005-2012. | the brown planthopper nilaparvata lugens and the whitebacked planthopper sogatella furcifera are both important pests on rice throughout asia. the major cause of recent outbreaks is thought to be the development of insecticide resistance. thus, the authors monitored insecticide susceptibilities in populations of these two insects immigrating into japan in the period 2005-2012. ten insecticides were tested, including members of the organophosphate, carbamate, pyrethroid, neonicotinoid and phenylp ... | 2014 | 23754555 |
| molecular cloning and characterization of a ryanodine receptor gene in brown planthopper (bph), nilaparvata lugens (stål). | ryanodine receptors (ryrs) are a distinct class of intracellular calcium (ca(2+)) release channel. the recent discovery of diamide insecticides has prompted studies on insect ryrs. however, information about the structure and function of insect ryrs is still limited. in this study, we isolated and characterized a full-length ryr cdna (named nlryr) from the brown planthopper, nilaparvata lugens (stål) (homoptera: delphacidae), a serious rice pest throughout asia. | 2014 | 23893901 |
| transcriptome analysis of neuropeptides and g-protein coupled receptors (gpcrs) for neuropeptides in the brown planthopper nilaparvata lugens. | the genes encoding neuropeptides, neurohormones and their putative g-protein coupled receptors were identified in the brown planthopper (bph), nilaparvata lugens (stål) by transcriptome analysis (rna-seq). forty-eight candidate genes were found to encode neuropeptides or peptide hormones. these include all known insect neuropeptides and neurohormones, with the exception of neuropeptide-like precursor 2 (nplp2) and trissin. the gene coding for prothoracicotropic hormone (ptth) was first identifie ... | 2014 | 23932938 |
| biological and biochemical characterization of a red-eye mutant in nilaparvata lugens (hemiptera: delphacidae). | a red-eye colony was established in our laboratory in brown planthopper (bph), nilaparvata lugens (stål), a major rice pest in asia. except for the red-eye phenotype, no other differences were observed between the wild-type (brown eye) and the mutant-type (red eye) in external characters. genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. biological studies found that egg production and egg viability in the red-eye mutant colony were not s ... | 2014 | 23955841 |
| multi-generational effects of rice harboring bph15 on brown planthopper, nilaparvata lugens. | the brown planthopper (bph), nilaparvata lugens, is one of the most devastating rice pests in asia. resistant cultivars are an effective way of managing bph. bph15 is a bph resistance gene and has been introgressed into rice variety minghui 63 (mh63). the multi-generational effects of rice line mh63::15 (harboring bph15) on bph were investigated and compared with its parental line mh63. | 2014 | 23589438 |
| cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect. | the brown planthopper (bph, nilaparvata lugens) is a destructive, monophagous, piercing-sucking insect pest of rice. previous studies indicated that jasmonic acid (ja) positively regulates rice defense against chewing insect pests but negatively regulates it against the piercing-sucking insect of bph. we here demonstrated that overexpression of allene oxide cyclase (aoc) but not opr3 (cis-12-oxo-phytodienoic acid (opda) reductase 3, an enzyme adjacent to aoc in the ja synthetic pathway) signific ... | 2014 | 25239066 |
| functional screen for micrornas of nilaparvata lugens reveals that targeting of glutamine synthase by mir-4868b regulates fecundity. | insect fecundity is regulated by the interaction of genotypes and the environment. micrornas (mirnas) also act in insect development and reproduction by regulating genes involved in these physiological processes. although hundreds of insect mirnas have been identified, the biological roles of most remain poorly understood. here, we used a multi-algorithm approach for mirna target prediction in 3'utrs of fecundity-related genes in the brown planthopper (bph) nilaparvata lugens and identified 38 p ... | 2015 | 26546713 |
| a salivary sheath protein essential for the interaction of the brown planthopper with rice plants. | salivary secretions, including gel saliva and watery saliva, play crucial roles in the interaction between the insect and plant during feeding. in this study, we identified a salivary gland-specific gene encoding a salivary sheath protein (nlshp) in nilaparvata lugens. nlshp has two alternative splicing variants; both are expressed at high levels during the nymph and adult stages. immunohistochemical staining showed that the nlshp were synthesized in the principal gland cells of the salivary gla ... | 2015 | 26483289 |
| intraspecific and interspecific variations in the mitochondrial genomes of nilaparvata (hemiptera: delphacidae). | planthoppers in the genus nilaparvata distant are serious pests of rice and many other crops in tropical and temperate asia, and northern australia. in this study, the mitochondrial genomes of four nilaparvata planthoppers were sequenced, three in nilaparvata lugens stål and one in nilaparvata muiri china. mitochondrial genome of nilaparvata contain the standard set of 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and a control region. the nucleotide composition of nilap ... | 2015 | 26470349 |
| analysis of the time-concentration-mortality response of nilaparvata lugens (hemiptera: delphacidae) to paichongding. | paichongding is an adicyclicnitromethylene neonicotinoid insecticide with a cis-configuration. bioassay of paichongding was conducted against nilaparvata lugens stål under a laboratory condition. mortality of n. lugens was analyzed by time-concentration-mortality (tcm) regressions based on the complementary log-log (cll) model. the conditional mortalities of test individuals increased with the exposure time after treatments with different concentrations, showing that the speed of insecticidal ac ... | 2015 | 26470347 |
| a comparative study on the population fitness of three strains of nilaparvata lugens (hemiptera: delphacidae) differ in eye color-related genes. | the brown planthopper, nilaparvata lugens (stål), is a destructive insect pest on rice throughout asia. as a visible genetic marker, red eye mutant colony of brown planthopper is a valuable material. here, we established the near-isogenic lines, nil-bb and nil-rr, through mating red eye females to brown eye brothers for eight successive generations. biological experiments showed that nil-bb had big fitness cost; however, nil-rr had comparable survival and fertility parameters with bb, a normal l ... | 2015 | 26470308 |
| changes in endosymbiotic bacteria of brown planthoppers during the process of adaptation to different resistant rice varieties. | the specific primers of five species of endosymbiotic bacteria were designed to determine their numbers in three virulent populations of brown planthopper, nilapavata lugens stål, and to assess changes during adaptation to different resistant varieties using fluorescent quantitative pcr. the results showed that chryseobacterium was the dominant bacteria in all three populations of brown planthopper, followed by acinetobacter in tn1 population, arsenophonus and serratia in mudgo population, and a ... | 2015 | 26313963 |
| the β-n-acetylhexosaminidase gene family in the brown planthopper, nilaparvata lugens. | β-n-acetylhexosaminidases (hexs) are enzymes that can degrade the chitin oligosaccharides that are produced by the activity of chitinases on chitin in insects. using bioinformatic methods based on genome and transcriptome databases, 11 β-n-acetylhexosaminidase genes (nlhexs) in nilaparvata lugens were identified and characterized. phylogenetic analysis revealed a six-grouped tree topology. the o-linked n-acetylglucosaminidase (o-glcnacase) group includes nlhex11, which harbours a catalytic domai ... | 2015 | 26304035 |
| reduction in mrna and protein expression of a nicotinic acetylcholine receptor α8 subunit is associated with resistance to imidacloprid in the brown planthopper, nilaparvata lugens. | target-site resistance is commonly caused by qualitative changes in insecticide target-receptors and few studies have implicated quantitative changes in insecticide targets in resistance. here we show that resistance to imidacloprid in a selected strain of nilaparvata lugens is associated with a reduction in expression levels of the nicotinic acetylcholine receptor (nachr) subunit nlα8. synergism bioassays of the selected strain suggested resistance was conferred, in part, by a target-site mecha ... | 2015 | 26259922 |
| genomic and transcriptomic insights into the cytochrome p450 monooxygenase gene repertoire in the rice pest brown planthopper, nilaparvata lugens. | the cytochrome p450 monooxygenase (p450) gene family is one of the most abundant eukaryotic gene families that encode detoxification enzymes. in this study, we identified an abundance of p450 gene repertoire through genome- and transcriptome-wide analysis in the brown planthopper (nilaparvata lugens), the most destructive rice pest in asia. detailed gene information including the exon-intron organization, size, transcription orientation and distribution in the genome revealed that many p450 loci ... | 2015 | 26234643 |
| silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens, sugar transporter gene 6 (nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. the influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of ph ... | 2015 | 26185058 |
| the multicopper oxidase gene family in the brown planthopper, nilaparvata lugens. | the multicopper oxidase (mco) family of enzymes includes laccases, ascorbate oxidases, bilirubin oxidases and a subgroup of metal oxidases. on the basis of a bioinformatics investigation, we identified 7 genes encoding putative multicopper oxidase proteins in the genome of the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae). mco1 and mco2 are conserved, while others diverse in insects. analysis of developmental and tissue-specific expression patterns revealed the following: ... | 2015 | 26107750 |
| dna methyltransferases have an essential role in female fecundity in brown planthopper, nilaparvata lugens. | dna methylation is an ancient epigenetic modification present in all three domains of life. however, the understanding of dna methylation in insects is limited. here, we amplified the full-length transcripts of the dna methyltransferases nlu-dnmt1 and nlu-dnmt3, indicating that a complete dna methylation toolkit exists in the brown planthopper, nilaparvata lugens, a destructive pest in rice production. nlu-dnmt1 and nlu-dnmt3 had the conserved motifs and domains of the dna methyltransferase fami ... | 2015 | 26043687 |
| activation of the tor signalling pathway by glutamine regulates insect fecundity. | the target of rapamycin (tor) positively controls cell growth in response to nutrients such as amino acids. however, research on the specific nutrients sensed by tor is limited. glutamine (gln), a particularly important amino acid involved in metabolism in organisms, is synthesised and catalysed exclusively by glutamine synthetase (gs), and our previous studies have shown that gln may regulate fecundity in vivo levels of the brown planthopper (bph) nilaparvata lugens. until now, it has remained ... | 2015 | 26024507 |
| two insulin receptors determine alternative wing morphs in planthoppers. | wing polyphenism is an evolutionarily successful feature found in a wide range of insects. long-winged morphs can fly, which allows them to escape adverse habitats and track changing resources, whereas short-winged morphs are flightless, but usually possess higher fecundity than the winged morphs. studies on aphids, crickets and planthoppers have revealed that alternative wing morphs develop in response to various environmental cues, and that the response to these cues may be mediated by develop ... | 2015 | 25799997 |
| differentially regulated genes in the salivary glands of brown planthopper after feeding in resistant versus susceptible rice varieties. | brown planthopper (bph) is a damaging insect pest of rice. we used suppression subtractive hybridization (ssh) and mirror orientation selection to identify differentially regulated genes in salivary glands of bph after feeding on resistant and susceptible varieties. the forward ssh library included 768 clones with insertions ranging from 250 to 1000 bp. after differential screening, a total of 112 transcripts were identified, which included 27 upregulated genes and seven downregulated genes. sev ... | 2015 | 25611813 |
| the genetic basis of population fecundity prediction across multiple field populations of nilaparvata lugens. | identifying the molecular markers for complex quantitative traits in natural populations promises to provide novel insight into genetic mechanisms of adaptation and to aid in forecasting population dynamics. in this study, we investigated single nucleotide polymorphisms (snps) using candidate gene approach from high- and low-fecundity populations of the brown planthopper (bph) nilaparvata lugens stål (hemiptera: delphacidae) divergently selected for fecundity. we also tested whether the populati ... | 2015 | 25581109 |
| construction of an immobilised acetylcholinesterase column and its application in screening insecticidal constituents from magnolia officinalis. | application of a matrix-immobilised target enzyme for screening inhibitors is widely used in drug development, but there are few studies in insecticide discovery. in this paper, an economical and effective immobilised acetylcholinesterase (ache) column was prepared using the sol-gel embedment method, which was further combined with high-performance liquid chromatography for screening the ache inhibitors and insecticidal compounds from complex natural products. | 2015 | 25228142 |
| chitinase-like gene family in the brown planthopper, nilaparvata lugens. | chitinases are important enzymes required for chitin degradation and reconstruction in insects. based on a bioinformatics investigation, we identified 12 genes encoding putative chitinase-like proteins, including 10 chitinases (cht), one imaginal disc growth factor (idgf) and one endo-β-n-acetylglucosaminidase (engase) in the genome of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). these 12 genes were clustered into nine different groups, with 11 in glycoside hydrolase famil ... | 2015 | 25224926 |
| structural characterization of the catalytic site of a nilaparvata lugens delta-class glutathione transferase. | glutathione transferases (gsts) are a major class of detoxification enzymes that play a central role in the defense against environmental toxicants and oxidative stress. here, we studied the crystal structure of a delta-class glutathione transferase from nilaparvata lugens, nlgstd, to gain insights into its catalytic mechanism. the structure of nlgstd in complex with glutathione, determined at a resolution of 1.7å, revealed that it exists as a dimer and its secondary and tertiary structures are ... | 2015 | 25497345 |
| herbivory-induced glucose transporter gene expression in the brown planthopper, nilaparvata lugens. | nilaparvata lugens, the brown planthopper (bph) feeds on rice phloem sap, containing high amounts of sucrose as a carbon source. nutrients such as sugars in the digestive tract are incorporated into the body cavity via transporters with substrate selectivity. eighteen sugar transporter genes of bph (nlst) were reported and three transporters have been functionally characterized. however, individual characteristics of nlst members associated with sugar transport remain poorly understood. comparat ... | 2015 | 26226652 |
| the effects of triazophos applied to transgenic bt rice on the nutritional indexes, nlvg expression, and population growth of nilaparvata lugens stål under elevated co₂. | the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae), is a typical pest in which population resurgence can be induced by insecticides. warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. however, the effects of elevated co2 (eco2) concentrations on the resurgence of n. lugens that have been treated with pesticides used for transgenic bt rice cultivation are not fully understood. the ... | 2015 | 25752430 |
| a new finely mapped oryza australiensis-derived qtl in rice confers resistance to brown planthopper. | brown planthopper (bph) is the most destructive pest of rice in asia. the bph resistance in the introgression line ir65482-17-511-5-7 (ir65482-17) is derived from the wild rice species oryza australiensis. an f2:3 population from a cross between zhenshan 97 (zs97) and ir65482-17 was used to map three quantitative trait loci (qtls) for seedling resistance and feeding rate to bph. the loci were distributed on chromosomes 2, 4 and 12. the qtl qbph4.2 on chromosome 4 had the largest effect, and cont ... | 2015 | 25682936 |
| molecular characterization and rna interference analysis of vitellogenin receptor from nilaparvata lugens (stål). | vitellogenin receptors (vgrs), members of the low-density lipoprotein receptor (ldlr) superfamily, are responsible for taking vitellogenin (vg) into developing oocytes. here the first full-length vgr cdna from a hemipteran insect, the brown planthopper (nilaparvata lugens), was cloned and sequenced. the complete mrna sequence was 6174 bp in length with an open reading frame (orf) of 5796 bp encoding 1931 amino acid residues. n. lugens vgr (nlvgr) contained two ligand-binding domains with five ld ... | 2015 | 25617689 |
| omics-based comparative transcriptional profiling of two contrasting rice genotypes during early infestation by small brown planthopper. | the small brown planthopper (sbph) is one of the destructive pests of rice. although different biochemical pathways that are involved in rice responding to planthopper infestation have been documented, it is unclear which individual metabolic pathways are responsive to planthopper infestation. in this study, an omics-based comparative transcriptional profiling of two contrasting rice genotypes, an sbph-resistant and an sbph-susceptible rice line, was assessed for rice individual metabolic pathwa ... | 2015 | 26633389 |
| differential interactions of 5-(4-piperidyl)-3-isoxazolol analogues with insect γ-aminobutyric acid receptors leading to functional selectivity. | γ-aminobutyric acid (gaba) receptors (gabars) mediate fast inhibitory synaptic transmission and are also targets for drugs and insecticides. to better understand the molecular interactions of ligands with the orthosteric sites of gabars, we examined 4-aryl/arylalkyl-5-(4-piperidyl)-3-isoxazolol, 4-aryl-5-(4-piperidyl)-3-isothiazolol, and 5-aryl-4-(4-piperidyl)-1-hydroxypyrazole for their antagonism with regard to three insect gabars. the 3-isoxazolol was preferable to the 3-isothiazolol and 1-hy ... | 2015 | 26453818 |
| identification of polymorphisms in cyrtorhinus lividipennis rdl subunit contributing to fipronil sensitivity. | as one of the most important predatory enemies, the miridbug, cyrtorhinus lividipennis, plays an important role in rice planthoppers control, such as nilaparvata lugens (brown planthopper). in order to compare insecticide selectivity between c. lividipennis and n. lugens, the contact acute toxicities of six insecticides (diazoxon, paraoxon, carbaryl, fenobucarb, fipronil and ethofenprox) were monitored. the results showed that all tested insecticides were more toxic to c. lividipennis than to n. ... | 2015 | 25619913 |
| identification of key amino acid differences between cyrtorhinus lividipennis and nilaparvata lugens nachr α8 subunits contributing to neonicotinoid sensitivity. | high sensitivity to neonicotinoid insecticides have been reported in the miridbug cyrtorhinus lividipennis, an important predatory enemy of rice planthoppers, such as nilaparvata lugens (brown planthopper). in the present study, the sensitivity of neonicotinoid insecticides between c. lividipennis and n. lugens were detected and compared. the results showed that neonicotinoid insecticides were much more toxic to the miridbug than to the brown planthopper. a nicotinic acetylcholine receptor subun ... | 2015 | 25613467 |
| phloem-exudate proteome analysis of response to insect brown plant-hopper in rice. | brown plant-hopper (nilaparvata lugens stål, bph), one of the most devastating agricultural insect pests of rice throughout asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. planting resistant variety is an efficient and economical way to control this pest. understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. here, we used an itraq-based quantitative proteomics approach to perform analysis of protein e ... | 2015 | 26072143 |
| genetic basis of multiple resistance to the brown planthopper (nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar 'asd7' (oryza sativa l. ssp. indica). | the rice cultivar asd7 (oryza sativa l. ssp. indica) is resistant to the brown planthopper (bph; nilaparvata lugens stål) and the green leafhopper (nephotettix virescens distant). here, we analyzed multiple genetic resistance to bph and the green rice leafhopper (grh; nephotettix cincticeps uhler). using two independent f2 populations derived from a cross between asd7 and taichung 65 (oryza sativa ssp. japonica), we detected two qtls (qbph6 and qbph12) for resistance to bph and one qtl (qgrh5) f ... | 2015 | 26719745 |
| application of brown planthopper salivary gland extract to rice plants induces systemic host mrna patterns associated with nutrient remobilization. | insect saliva plays an important role in modulation of plant-insect interactions. although this area of research has generated much attention in recent years, mechanisms of how saliva affects plant responses remain poorly understood. to address this void, the present study investigated the impact of the brown planthopper (nilaparvata lugens, stål; hereafter bph) salivary gland extract (sge) on rice (oryza sativa) systemic responses at the mrna level. differentially expressed rice mrnas were gene ... | 2015 | 26641488 |
| molecular cloning and characterization of the putative halloween gene phantom from the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in insect postembryonic development and reproduction. several cytochrome p450 mono-oxygenases (cyps), encoded by the halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in diptera, lepidoptera and orthoptera. here the putative halloween gene phantom (phm, cyp306a1) from a hemipteran insect species, the small brown planthopper laodelphax striatellus, was cloned. lsphm shows five insect ... | 2015 | 24954278 |
| a halloween gene shadow is a potential target for rna-interference-based pest management in the small brown planthopper laodelphax striatellus. | laodelphax striatellus is an economically important rice pest in china. ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. the cytochrome p450 monooxygenase shadow (sad) plays a critical role in ecdysteroidogenesis. here, tests were conducted to establish whether lssad was a potential target gene for rna-interference-based management of l. striatellus. | 2015 | 24648012 |
| knockdown of the ionotropic γ-aminobutyric acid receptor (gabar) rdl gene decreases fipronil susceptibility of the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | insect γ-aminobutyric acid receptors (gabars) are important molecular targets of cyclodiene and phenylpyrazole insecticides. previously gabars encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. in this study, we cloned the open reading frame cdna sequence of rdl gene from fipronil-susceptible and fipronil-resistant strains of laodelphax striatellus (lsrdl). sequence analysis confirmed the pre ... | 2015 | 25808850 |
| take-off time of the first generation of the overwintering small brown planthopper, laodelphax striatellus in the temperate zone in east asia. | overseas migration of the small brown planthopper, laodelphax striatellus (fallén), occurs during the winter wheat harvest season in east asia. knowing the take-off time of emigrating l. striatellus is crucial for predicting such migrations with a simulation technique because winds, carriers of migratory insects, change continuously. several methods were used in china and japan from late may to early june 2012 and again in 2013 to identify the precise timing of take-off. these methods included: ... | 2015 | 25780936 |
| evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, laodelphax striatellus. | understanding dispersal ability in pest species is critical for both theoretical aspects of evolutionary and population biology and from a practical standpoint, such as implementing effective forecasting systems. the small brown planthopper (sbph), laodelphax striatellus (fallén), is an economically important pest, but few data exist on its dispersal ability. here, we used mitochondrial and nuclear markers to elucidate the population genetic structure of sbph and of the parasitic bacterium wolba ... | 2015 | 25622966 |
| probing stimulants from the rice plant towards the smaller brown planthopper, laodelphax striatellus (fallen) (homoptera: delphacidae). | when adult males of the smaller brown planthopper, laodelphax striatellus were fed on 2% crude rice (leaf and stem) extract containing 15% sucrose there were characteristic stylet sheaths deposited on parafilm membrane. further bioassays with the butanol-soluble fr. of the extract revealed that it is highly effective for the insects. when the butanol fr. was charged on an ods open column and eluted in sequence with 20, 40 and 100% methanol in water, the ods-40% methanol fr. was shown as the most ... | 2015 | 11204184 |
| roles of nlaktip in the growth and eclosion of the rice brown planthopper, nilaparvata lugens stål, as revealed by rna interference. | akt-interacting protein (aktip) interacts with serine/threonine protein kinase b (pkb)/akt. aktip modulates akt's activity by enhancing the phosphorylation of the regulatory site and plays a crucial role in multiple biological processes. in this study, the full length cdna of nlaktip, a novel aktip gene in the brown planthopper (bph) nilaparvata lugens, was cloned. the reverse transcription quantitive pcr (rt-qpcr) results showed that the nlaktip gene was strongly expressed in gravid female adul ... | 2015 | 26402675 |
| small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety. | pyramiding multiple genes into a desirable genetic background can take years to accomplish. in this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. pink3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (sub) and susceptible to bacterial leaf blight (bb), leaf-neck blast (bl) and the brown planthopper (bph)-was used as a genetic basis for significant improvements through gene pyramiding. | 2015 | 25844112 |
| comparing gene expression profiles between bt and non-bt rice in response to brown planthopper infestation. | bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. we previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (bph) fed on bt rice line kmd2, although bt insecticidal protein cry1ab could rarely concentrate in this non-target rice pest. in the present study, we performed microarray analysis in an effort to detect bt-independent variation, which might render bt rice more defensive and/or less n ... | 2015 | 26734057 |
| identification and function analysis of enolase gene nleno1 from nilaparvata lugens (stål) (hemiptera:delphacidae). | the enolase [ec 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-pge) to phosphoenolpyruvate (pep). in this study, a full-length cdna encoding α-enolase was cloned from rice brown planthopper (nilaparvata lugens) and is provisionally designated as nleno1. the cdna sequence of nleno1 was 1,851 bp with an open reading frame (orf) of 1,305 bp and encoding 434 amino acids. the deduced protein shares high identity of 80-87% with eno1-like p ... | 2015 | 26056319 |
| pathways of amino acid degradation in nilaparvata lugens (stål) with special reference to lysine-ketoglutarate reductase/saccharopine dehydrogenase (lkr/sdh). | nilaparvata lugens harbors yeast-like symbionts (ylss). in present paper, a genome-wide analysis found 115 genes from ni. lugens and 90 genes from ylss that were involved in the metabolic degradation of 20 proteinogenic amino acids. these 205 genes encoded for 77 enzymes. accordingly, the degradation pathways for the 20 amino acids were manually constructed. it is postulated that ni. lugens can independently degrade fourteen amino acids (threonine, alanine, glycine, serine, aspartate, asparagine ... | 2015 | 26000452 |
| insectbase: a resource for insect genomes and transcriptomes. | the genomes and transcriptomes of hundreds of insects have been sequenced. however, insect community lacks an integrated, up-to-date collection of insect gene data. here, we introduce the first release of insectbase, available online at http://www.insect-genome.com. the database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 mirnas of 69 insects, 96,925 pirnas of drosophila melanogaster and chilo suppressalis, 2439 lncrna of n ... | 2015 | 26578584 |
| ran involved in the development and reproduction is a potential target for rna-interference-based pest management in nilaparvata lugens. | ran (rangtpase) in insects participates in the 20-hydroxyecdysone signal transduction pathway in which downstream genes, ftz-f1, krüppel-homolog 1 (kr-h1) and vitellogenin, are involved. a putative ran gene (nlran) was cloned from nilaparvata lugens, a destructive phloem-feeding pest of rice. nlran has the typical ran primary structure features that are conserved in insects. nlran showed higher mrna abundance immediately after molting and peaked in newly emerged female adults. among the examined ... | 2015 | 26554926 |
| rnai knockdown of acetyl-coa carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, nilaparvata lugens. | a major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. this study focused on the effects of the antibiotic, jinggangmycin (jgm), a fungicide widely used in asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (bph) nilaparvata lugens and the white-backed planthopper (wbph) sogatella furcifera, both serious resurgence rice pests ... | 2015 | 26482193 |
| rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant. | most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ... | 2015 | 26073458 |
| transgenic cry1c or cry2a rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | transgenic rice producing the insecticidal protein from bacillus thuringiensis berliner (bt) is protected from damage by lepidopteran insect pests. however, one of the main concerns about bt rice is the potential impact on non-target herbivores. in the present study, the ecological impacts of two bt rice lines, t1c-19 expressing cry1c protein and t2a-1 expressing cry2a protein, on the non-target herbivore brown planthopper (bph), nilaparvata lugens (stål), were evaluated under laboratory and fie ... | 2015 | 25067834 |
| genome-wide identification of long noncoding rna genes and their potential association with fecundity and virulence in rice brown planthopper, nilaparvata lugens. | the functional repertoire of long noncoding rna (lncrna) has been characterized in several model organisms, demonstrating that lncrna plays important roles in fundamental biological processes. however, they remain largely unidentified in most species. understanding the characteristics and functions of lncrna in insects would be useful for insect resources utilization and sustainable pest control. | 2015 | 26437919 |
| apterous a modulates wing size, bristle formation and patterning in nilaparvata lugens. | apterous a (apa), a member of the lim-homeobox gene family, plays a critical role in the development of wing. the achaete-scute complex (as-c) encodes basic helix-loop-helix (bhlh) transcription factors and functions in bristle development. in the present study, we cloned apa (nlapa) and an achaete-scute homologue (nlash) from n. lugens. levels of nlapa and nlash were higher in nymphs than adults, with particularly high expression in the thorax of nymphs. nlapa expressed more highly in nymphs of ... | 2015 | 25995006 |
| rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3. | the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ... | 2015 | 25653432 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| molecular cloning and expression profile of an atp-binding cassette (abc) transporter gene from the hemipteran insect nilaparvata lugens. | the atp-binding cassette (abc) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. in insects, abc transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. in this study, the nilaparvata lugens stal (hemiptera: delphacidae) abcg (nlabcg) gene was identified and characterized. the complete mrna sequence of nlabcg was 2608-bp long, with a ... | 2015 | 25867414 |
| rna interference of nadph-cytochrome p450 reductase of the rice brown planthopper, nilaparvata lugens, increases susceptibility to insecticides. | nadph-cytochrome p450 reductase (cpr) is essential for numerous biological reactions catalysed by microsomal cytochrome p450 monooxygenases (p450s). knockdown of cpr in several insects leads to developmental defects and increased susceptibility to insecticides. however, information about the role of cpr in the brown planthopper, nilaparvata lugens, is still unavailable. | 2015 | 24515640 |
| tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-ones as novel neonicotinoid insecticides: reaction selectivity and substituent effects on the activity level. | tetraheterocyclic tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-one derivatives as novel neonicotinoid candidates were designed and prepared by selective etherification, chlorination and esterification of ninhydrin-heterocyclic ketene aminals adducts. some of the designed compounds showed excellent insecticidal activity against cowpea aphids (aphis craccivora), brown planthopper (nilaparvata lugens), and armyworm (mythimna separata). in particular, the activity against armyworm (mythim ... | 2015 | 25611859 |
| molecular analysis of oryza latifolia desv. (ccdd genome)-derived introgression lines and identification of value-added traits for rice (o. sativa l.) improvement. | oryza latifolia is a tetraploid wild oryza species with a ccdd genome that has been reported to harbor resistance to bacterial blight (bb), brown planthopper, and whitebacked planthopper. aside from these traits, o. latifolia is also being tapped as a new source of resistance to lodging and high biomass production. to explore the genetic potential of o. latifolia as a novel genetic resource for the improvement of existing o. sativa cultivars, 27 disomic derivatives of o. latifolia monosomic alie ... | 2015 | 24939891 |
| genomic analysis of an ascomycete fungus from the rice planthopper reveals how it adapts to an endosymbiotic lifestyle. | a number of sap-sucking insects harbor endosymbionts, which are thought to play an important role in the development of their hosts. one of the most important rice pests, the brown planthopper (bph), nilaparvata lugens (stål), harbors an obligatory yeast-like symbiont (yls) that cannot be cultured in vitro. genomic information on this yls would be useful to better understand its evolution. in this study, we performed genome sequencing of the yls using both 454 and illumina approaches, generating ... | 2015 | 26338189 |
| rna interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae). | the brown planthopper nilaparvata lugens is a serious phloem-feeding pest of rice in china. the current study focuses on a saccharopine dehydrogenase (sdh) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (lys), which plays a role in insect growth and carnitine production (as a substrate). the protein, provisionally designated as nlylssdh [a sdh derived from yeast-like symbiont (yls) in n. lugens], had a higher transcript level in abdomens, compared with heads, wi ... | 2015 | 25908053 |
| pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (laodelphax striatellus). | yeast-like symbionts (yls) are endosymbionts that promote the growth of delphacid planthoppers (hemiptera: delphacidae), some of which are pests on cultivated rice. identification and characterization of yls growth can be helpful for pest control, because it has been demonstrated that there is a variety of yls in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. so, elucidation of the species of yls in planthopper is crucial for exploiting a new way to con ... | 2015 | 25499750 |
| map-based cloning and characterization of bph29, a b3 domain-containing recessive gene conferring brown planthopper resistance in rice. | rice (oryza sativa l.) production, essential for global food security, is threatened by the brown planthopper (bph). the breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. an indica rice introgression line rbph54, derived from wild rice oryza rufipogon, has been identified with sustainable resistance to bph, which is governed by recessive alleles at two loci. in this study, a map-b ... | 2015 | 26136269 |
| rna interference-mediated silencing of a halloween gene spookier affects nymph performance in the small brown planthopper laodelphax striatellus. | post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. halloween gene spookier (spok, cyp307a2) has been documented to be involved in ecdysteroidogenesis in drosophila melanogaster and bombyx mori. we describe here the cloning and characterization of halloween gene spookier (lsspok, lscyp307a2) in the small brown planthopper laodelphax striatellus, a hemipteran insect species. lsspok has three insect-conserved p450 motifs, that is, helix-k, perf moti ... | 2015 | 24282064 |
| effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study. | bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ... | 2015 | 26470122 |
| prioritizing plant defence over growth through wrky regulation facilitates infestation by non-target herbivores. | plants generally respond to herbivore attack by increasing resistance and decreasing growth. this prioritization is achieved through the regulation of phytohormonal signaling networks. however, it remains unknown how this prioritization affects resistance against non-target herbivores. in this study, we identify wrky70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with w-box motives and prioritizes defence over gro ... | 2015 | 26083713 |
| bicaudal-c plays a vital role in oogenesis in nilaparvata lugens (hemiptera: delphacidae). | bicaudal-c (bic-c) was originally identified in a drosophila melanogaster mutagenesis screen and plays vital roles in embryogenesis. in this study, we characterized the bic-c gene in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae), an insect pest that undergoes incomplete metamorphosis. our result showed that n. lugens bic-c (nlbic-c) is a female-specific gene in this species. it is specifically expressed in developing oocytes and is not expressed in laid eggs. ribonucleic aci ... | 2015 | 26025198 |
| differential mechanisms of action of the novel γ-aminobutyric acid receptor antagonist ectoparasiticides fluralaner (a1443) and fipronil. | fluralaner (a1443) is an isoxazoline ectoparasiticide that is a novel antagonist of γ-aminobutyric acid (gaba) receptors (gabars), with a potency comparable to that of fipronil, a phenylpyrazole ectoparasiticide. to clarify the biological effectiveness of fluralaner against fipronil-resistant pests, differences in the actions of fluralaner and fipronil on gabars that possess resistance to dieldrin (rdl)-type mutations were evaluated. | 2015 | 24591229 |
| novel hydrazone derivatives containing pyridine amide moiety: design, synthesis, and insecticidal activity. | a series of novel hydrazone derivatives containing pyridine amide moiety were designed, synthesized, and evaluated for their insecticidal activity. bioassays indicated that some of the target compounds exhibited good insecticidal activities against nilaparvata lugens (n. lugens), plutella xylostella (p. xylostella), mythimna separata (m. separata), helicoverpa armigera (h. armigera), pyrausta nubilalis (p. nubilalis), and culex pipiens pallens (c. pipiens pallens). in particular, compound 5j rev ... | 2016 | 26832221 |