Publications

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transformation of litchi pericarp-derived condensed tannin with aspergillus awamori.condensed tannin is a ubiquitous polyphenol in plants that possesses substantial antioxidant capacity. in this study, we have investigated the polyphenol extraction recovery and 2,2-diphenyl-1-picrylhydrazyl (dpph) scavenging activity of the extracted polyphenol after litchi pericarp is treated with aspergillus awamori, aspergillus sojae or aspergillus oryzae. we have further explored the activity of a. awamori in the formation of condensed tannin. the treatment of a. awamori appeared to produce ...201627420043
biohydrogen production in the suspended and attached microbial growth systems from waste pastry hydrolysate.waste pastry was hydrolyzed by glucoamylase and protease which were obtained from solid state fermentation of aspergillus awamori and aspergillus oryzae to produce waste pastry hydrolysate. then, the effects of hydraulic retention times (hrts) (4-12h) on hydrogen production rate (hpr) in the suspended microbial growth system (continuous stirred tank reactor, cstr) and attached microbial growth system (continuous mixed immobilized sludge reactor, cmisr) from waste pastry hydrolysate were investig ...201627416509
species distribution and in vitro azole susceptibility of aspergillus section nigri isolates from clinical and environmental settings.aspergillus section nigri includes species of interest for animal and human health, although studies on species distribution are limited to human cases. data on the antifungal susceptibilities and the molecular mechanism of triazole resistance in strains belonging to this section are scant. forty-two black aspergillus strains from human patients (16 isolates), animals (14 isolates), and the environment (12 isolates) were molecularly characterized and their in vitro triazole susceptibilities inve ...201627413191
valorization of bakery waste for biocolorant and enzyme production by monascus purpureus.a concept of utilizing bakery waste as a nutrient source for the fermentative production of bio-colorant by monasucus purpureus has been developed. the proposed ideas provide an innovative approach to establish a system or method to reduce the bakery waste problem encountered by hong kong or other countries. bakery waste collected from bakery store was used in submerged and solid-state fermentation of monascus purpureus to produce bio-colorant, which could potentially be applied in food and text ...201627208753
continuous biohydrogen production from waste bread by anaerobic sludge.in this study, continuous biohydrogen production from waste bread by anaerobic sludge was performed. the waste bread was first hydrolyzed by the crude enzymes which were generated by aspergillus awamori and aspergillus oryzae via solid-state fermentation. it was observed that 49.78g/l glucose and 284.12mg/l free amino nitrogen could be produced with waste bread mass ratio of 15% (w/v). the waste bread hydrolysate was then used for biohydrogen production by anaerobic sludge in a continuous stirre ...201627065225
aspergillus tubingensis and aspergillus niger as the dominant black aspergillus, use of simple pcr-rflp for preliminary differentiation.this work aimed to identify the species distribution of common clinical and environmental isolates of black aspergilli based on simple restriction fragment length polymorphism (rflp) analysis of the β-tubulin gene. a total of 149 clinical and environmental strains of black aspergilli were collected and subjected to preliminary morphological examination. total genomic dnas were extracted, and pcr was performed to amplify part of the β-tubulin gene. at first, 52 randomly selected samples were spec ...201626852194
prospecting for the incidence of genes involved in ochratoxin and fumonisin biosynthesis in brazilian strains of aspergillus niger and aspergillus welwitschiae.aspergillus niger "aggregate" is an informal taxonomic rank that represents a group of species from the section nigri. among a. niger "aggregate" species aspergillus niger sensu stricto and its cryptic species aspergillus welwitschiae (=aspergillus awamori sensu perrone) are proven as ochratoxin a and fumonisin b2 producing species. a. niger has been frequently found in tropical and subtropical foods. a. welwitschiae is a new species, which was recently dismembered from the a. niger taxon. these ...201626803270
optimization of d-lactic acid production using unutilized biomass as substrates by multiple parallel fermentation.this study investigated the optimization of d-lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (mpf) with leuconostoc mesenteroides and aspergillus awamori. the factors involved in mpf that were assessed in this study comprised banana peel and corncob, kh2po4, tween 80, mgso4·7h2o, nacl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for d-lactic acid production. optimization of these comp ...201628330258
immobilization of halophilic aspergillus awamori em66 exochitinase on grafted k-carrageenan-alginate beads.a novel extreme halophilic exochitinase enzyme was produced by honey isolate aspergillus awamori em66. the enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (ca) with 93 % immobilization yield. the immobilization process significantly improved the enzyme specific activity 2.6-fold compared to the free form. the significant factors influencing the immobilization process such as enzyme protein concentration and loading time were studied. distinguishable characteristics of o ...201628330097
[influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori].the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ...201625427372
[influence of uv-radiation on physical-chemical and functional properties of free immobilized glucoamylase from aspergillus awamori].the effect of uv light (240-390 nm) at the doses of 151-1510 j/m2 on the catalytic activity of free and im- mobilized glucoamylase from aspergillus awamori was investigated. it was established that the loss of catalytic activity of the enzyme was associated with photochemical transformations of trp-120, which is part of the active site of glucoamylase. it was shown that the use of collagen as a carrier for immobilization of glucoamylase reduces the constant photoinactivation of the enzyme. it wa ...201625507622
bioactive profile, free-radical scavenging potential, dna damage protection activity, and mycochemicals in aspergillus awamori (mtcc 548) extracts: a novel report on filamentous fungi.biomass of aspergillus awamori was investigated for mycochemicals, total phenolic compounds (tpc), condensed tannin content (ctc), free-radical scavenging potential (frsp), and dna damage protection activity. frsp was determined using dpph, abts, frap (ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. water (aq), aqueous ethanol 50% (aqe), and methanol were used as extraction phase at 44.5 °c for 23.8 min. aqe shows the presence of ma ...201728660460
production, immobilization and thermodynamic studies of free and immobilized aspergillus awamori amylase.enzyme cost, stability and its thermodynamic characteristics are the main criteria for industrial use. in this study, aspergillus awamori amylase was constitutively produced using various agro-industrial wastes. olive oil cake gave the highest activity (230u/g). the amylase was partially purified to 2.81-fold purification. immobilization was achieved using different carriers by covalent binding. the novel carrier ca(+2) alginate (alg) starch (st)/polyethyleneimine (pei)/glutaraldehyde (ga), show ...201728438682
enhanced biodiesel production through phyco-myco co-cultivation of chlorella minutissima and aspergillus awamori: an integrated approach.algae-fungus co-culture was investigated as an alternative biodiesel feedstock. an oleaginous filamentous fungus aspergillus awamori was co-cultured with chlorella minutissima mcc 27 and chlorella minutissima utex 2219, respectively in n11 medium furnished with different carbon and nitrogen sources. the biomass and lipid production potential of the two c. minutissima-a. awamori co-cultures was compared against the monocultures. a substantial enhancement in biomass and lipid accumulation was obse ...201728475992
biochemical characterization of the xylan hydrolysis profile of the extracellular endo-xylanase from geobacillus thermodenitrificans t12.endo-xylanases are essential in degrading hemicellulose of various lignocellulosic substrates. hemicellulose degradation by geobacillus spp. is facilitated by the hemicellulose utilization (hus) locus that is present in most strains belonging to this genus. as part of the hus locus, the xyna gene encoding an extracellular endo-xylanase is one of the few secreted enzymes and considered to be the key enzyme to initiate hemicellulose degradation. several geobacillus endo-xylanases have been charact ...201728521816
the role of soybean extracts and isoflavones in hormone-dependent breast cancer: aromatase activity and biological effects.estrogen receptor-positive (er(+)) breast cancer is the most common cause of cancer death in women worldwide. nowadays, the relationship between soya diet and breast cancer is controversial due to the unknown role of its isoflavones, genistein (g) and daidzein (d). in this work, we investigated not only the anti-tumor properties of a soybean extract (nbse) but also whether the biotransformation of extract (bse) by the fungus aspergillus awamori increased its effectiveness. the bse showed a stron ...201728644496
aspergillus awamori-fermented mung bean seed coats enhance the antioxidant and immune responses of weaned pigs.the potential benefits of aspergillus-fermented mung bean seed coats (fmsc) for weaned pigs remain unexplored. both in vitro and in vivo experiments were employed to evaluate the potential of fmsc supplement on the growth, antioxidant and immune responses of weaned pigs. the total polyphenols and dpph scavenging capability of ethanol extract of fmsc exhibited a greater (p < 0.01) increase than those of pre-fermentation. with the addition of the polyphenol of fmsc extract, an increase in phagocyt ...201728063238
Displaying items 401 - 417 of 417