Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| survival of stenotrophomonas maltophilia following exposure to concentrations of tobramycin used in aerosolized therapy for cystic fibrosis patients. | the use of aerosolized tobramycin is an important component in the management of patients with cystic fibrosis, particularly those with chronic pseudomonas aeruginosa infection. clinicians have been concerned that long-term therapy with aerosolized tobramycin may increase the risk of colonization with multi-resistant bacteria, including stenotrophomonas maltophilia. the ability of five strains (three clinical, two environmental) of s. maltophilia to survive exposure to 16000 microg/ml tobramycin ... | 2001 | 11137651 |
| analysis of bacterial community structure in sulfurous-oil-containing soils and detection of species carrying dibenzothiophene desulfurization (dsz) genes. | the selective effects of sulfur-containing hydrocarbons, with respect to changes in bacterial community structure and selection of desulfurizing organisms and genes, were studied in soil. samples taken from a polluted field soil (a) along a concentration gradient of sulfurous oil and from soil microcosms treated with dibenzothiophene (dbt)-containing petroleum (fsl soil) were analyzed. analyses included plate counts of total bacteria and of dbt utilizers, molecular community profiling via soil d ... | 2001 | 11229891 |
| [digestive tract microbiota in female lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae) from colonies feeding on blood meal and sucrose plus blood meal]. | there are very few reports on the microbiota of the digestive tract of sand flies, an important omission considering that blood is not the only meal ingested. male and female sand flies obtain sugar meals from several sources, thereby increasing their chance of infection with microorganisms. chances of contamination are higher when insects are bred in the laboratory, and this may affect the development of leishmania spp. from the digestive tract of 300 sand fly females separated in two groups we ... | 2001 | 11241946 |
| comparative activities of isepamicin, amikacin, cefepime, and ciprofloxacin alone or in combination with other antibiotics against stenotrophomonas maltophilia. | 2001 | 11245333 | |
| the problem of a solvent exposable disulfide when preparing co(ii)-substituted metallo-beta-lactamase l1 from stenotrophomonas maltophilia. | in an effort to prepare co(ii)-substituted metallo-beta-lactamase l1 from stenotrophomonas maltophilia for future spectroscopic and mechanistic studies, two methods for the preparation of co(ii)-l1 were tested. method a involved adding cocl2 directly to apo-l1 under anaerobic conditions. the resulting enzyme contained 1.9 moles of cobalt and exhibited very little activity, and uv-vis, 1h nmr, and epr studies indicated that most of the cobalt in this sample was co(iii). method b involved reducing ... | 2001 | 11191226 |
| comparison of serum bactericidal activity of ceftazidime, ciprofloxacin and meropenem against stenotrophomonas maltophilia. | 2001 | 11152445 | |
| plasmid location and molecular heterogeneity of the l1 and l2 beta-lactamase genes of stenotrophomonas maltophilia. | an approximately 200-kb plasmid has been purified from clinical isolates of stenotrophomonas maltophilia. this plasmid was found in all of the 10 isolates examined and contains both the l1 and the l2 beta-lactamase genes. the location of l1 and l2 on a plasmid makes it more likely that they could spread to other gram-negative bacteria, potentially causing clinical problems. sequence analysis of the 10 l1 genes revealed three novel genes, l1c, l1d, and l1e, with 8, 12, and 20% divergence from the ... | 2001 | 11158734 |
| the in vitro activity of sulbactam combined with third generation cephalosporins against third generation cephalosporin-resistant bacteria. | the in vitro activity of the beta-lactamase inhibitor sulbactam combined with cefuroxime, cefotaxime or ceftazidime in the ratio of 1:1 was studied against ceftazidime- or cefuroxime-resistant gram-negative rods and staphylococcus aureus. sulbactam enhanced the antibacterial activities of cefuroxime, cefotaxime and ceftazidime against gram-negative rods. the mic(90) of ceftazidime against escherichia coli, enterobacter cloacae, citrobacter freundii, acinetobacter spp. and pseudomonas aeruginosa ... | 2001 | 11165119 |
| resistance patterns among nosocomial pathogens: trends over the past few years. | multiple surveillance studies have demonstrated that resistance among prevalent pathogens is increasing at an alarming rate, leading to greater patient morbidity and mortality from nosocomial infections. among gram-positive organisms, the most important resistant pathogens are methicillin- (oxacillin-)resistant staphylococcus aureus, beta-lactam-resistant and multidrug-resistant pneumococci, and vancomycin-resistant enterococci. important causes of gram-negative resistance include extended-spect ... | 2001 | 11171776 |
| metallo-beta-lactamase producers in environmental microbiota: new molecular class b enzyme in janthinobacterium lividum. | eleven environmental samples from different sources were screened for the presence of metallo-beta-lactamase-producing bacteria by using a selective enrichment medium containing a carbapenem antibiotic and subsequently testing each isolate for production of edta-inhibitable carbapenemase activity. a total of 15 metallo-beta-lactamase-producing isolates, including 10 stenotrophomonas maltophilia isolates, 3 chryseobacterium spp., one aeromonas hydrophila isolate, and one janthinobacterium lividum ... | 2001 | 11181369 |
| structures of the o4 and o18 antigens of stenotrophomonas maltophilia: a case of enantiomeric repeating units. | the o-specific side-chain polymers of lipopolysaccharides from the reference strains for stenotrophomonas maltophilia serogroups 04 and o18 are both xylosylated rhamnans. in the 04 polymer, both sugar components are the d isomers, whereas the o18 polymer contains only the l isomers. by means of nmr spectroscopy, methylation analysis and smith degradation, the repeating unit of the 04 polymer was identified as a doubly-branched pentasaccharide of the structure shown below. the o18 polymer is base ... | 2001 | 11217974 |
| structure of the o16 antigen of stenotrophomonas maltophilia. | a polysaccharide containing d-ribose, n-acetyl-d-glucosamine, and n-acetyl-d-mannosamine was isolated from the phenol-soluble lipopolysaccharide extracted from defatted cell walls of the reference strain (560) for serogroup o16 of stenotrophomonas maltophilia. the results of methylation analysis, chemical degradations, and nmr spectroscopy showed that the polysaccharide is based on a branched trisaccharide repeating-unit of the structure shown below. although ribose was absent from about half of ... | 2001 | 11217982 |
| acral necrosis by stenotrophomonas maltophilia. | stenotrophomonas maltophilia (sm) has been considered a nosocomial pathogen. nevertheless, community acquired infection may occur more frequently than usually recognized. | 2001 | 11730046 |
| electronic ventilator temperature sensors as a potential source of respiratory tract colonization with stenotrophomonas maltophilia. | stenotrophomonas maltophilia (s. maltophilia) is an important cause of nosocomial infection among ventilated and immunocompromised patients, and among patients receiving broad-spectrum antimicrobials. we report a cluster of patients in a surgical intensive care unit who were colonized or infected with s. maltophilia. an epidemiological investigation was initiated after surveillance data revealed that eight patients were culture-positive from sputum for s. maltophilia in the preceding month. revi ... | 2001 | 11740879 |
| a novel metallo-beta-lactamase, mbl1b, produced by the environmental bacterium caulobacter crescentus. | caulobacter crescentus 101123 possesses a gene (mbl1b) encoding a metallo-beta-lactamase with 32% amino acid identity to the l1 metallo-beta-lactamase from stenotrophomonas maltophilia. the gene was cloned into an expression vector and the enzyme, mbl1b, was expressed in escherichia coli. mbl1b was purified. catalytic properties for several antibiotics were determined. the enzyme exhibits michaelis-menten kinetics for imipenem, meropenem and nitrocefin but substrate inhibition kinetics with cefo ... | 2001 | 11749954 |
| burkholderia/stenotrophomonas. | 2001 | 11764736 | |
| weeds as a source of plant growth promoting rhizobacteria in agricultural soils. | the influence of plant growth promoting (pgp) activity of bacterial communities recovered from each of six weed species (barnyard grass (echinochloa crusfalli (l.) beauv.), corn spurrey (spergula arvensis l.), goldenrod (sonchus sp.), italian ryegrass (lolium multiflorum l.), lamb's-quarters (chenopodium album l.), and quack grass (agropyron repens (l.) beauv.)) was examined in relation to the effect it had on the growth of the potato cultivar russet burbank. bacterial species composition and co ... | 2001 | 11766050 |
| distribution and prevalence of antimicrobial resistance among gram-negative isolates in intensive care units (icu) in belgian hospitals between 1996 and 1999. | to assess the distribution and prevalence of resistance rates among gram-negative isolates in belgian intensive care units (icus) between 1996 and 1999. | 2001 | 11770225 |
| [utility of prolonged incubation and terminal subcultures of blood cultures from immunocompromised patients]. | the value of blind terminal subcultures (7 and 30 days) and prolonged incubation (30 days) of blood cultures from immunosuppressed patients was analyzed in the fundación favaloro, the fundación para la lucha contra las enfermedades neurológicas de la infancia and the hospital de niños ricardo gutiérrez. a total of 2707 blood cultures and 369 patients were included (transplantation of solid organs 154, oncohematologic disorders 106 and solid tumors 109). bact-alert bottles were incubated at 35 de ... | 2001 | 11594009 |
| synergistic activities of gatifloxacin in combination with other antimicrobial agents against pseudomonas aeruginosa and related species. | drug combinations have been used to treat serious infections caused by pseudomonas, burkholderia, stenotrophomonas, and acinetobacter. in this study, the combined drug effects of gatifloxacin (gat) and nonquinolones were determined by time-kill analysis at clinically achievable drug concentrations. synergy (>or=2 log(10)-enhanced killing at 24 h) was observed with gat plus amikacin or a beta-lactam against 50 to 75% of strains, including strains nonsusceptible to one or both drugs. | 2001 | 11600384 |
| in vitro activity of moxifloxacin against common clinical bacterial isolates in taiwan. | the in vitro antimicrobial activities of moxifloxacin were compared with 7 other antimicrobial agents. a total of 707 isolates of 11 common pathogenic bacteria were collected from the national taiwan university hospital; antimicrobial activities against these isolates were evaluated by minimum inhibitory concentration using an agar-dilution method. most common pathogenic bacteria were susceptible to moxifloxacin, including methicillin-susceptible and -resistant staphylococcus aureus, methicillin ... | 2001 | 11605808 |
| [new aspects of antibiotic resistance and possibilities of its prevention]. | new phenomena of the antibiotic resistance in bacteria have recently appeared. the may hold present explosive development of resistance and prevent its transferability from multiple drug resistant bacteria to still sensitive ones. they may prevent the production of so-called extended-spectrum beta-lactamases (esbls) among enterobacteriaceae producing resistance virtually to all penicillins and cephalosporins with exception of those antibiotics potentiated by clavulanic acid or sulbactam, the res ... | 2001 | 11678026 |
| cell fingerprinting: an approach to classifying cells according to mass profiles of digests of protein extracts. | we present a statistical framework for classifying cells according to the set of peptide masses obtained by mass spectrometric analysis of digestions of whole cell protein extracts. the digest is separated by high performance liquid chromatography (hplc) coupled directly to a mass spectrometer either by an electrospray interface or by collection to a matrix-assisted laser desorption/ionization target plate. here, the mass to charge ratio, intensity, and hplc retention time of the peptides are me ... | 2001 | 11678037 |
| identification and antimicrobial susceptibility of alcaligenes xylosoxidans isolated from patients with cystic fibrosis. | in the past decade, potential pathogens, including alcaligenes species, have been increasingly recovered from cystic fibrosis (cf) patients. accurate identification of multiply antibiotic-resistant gram-negative bacilli is critical to understanding the epidemiology and clinical implications of emerging pathogens in cf. we examined the frequency of correct identification of alcaligenes spp. by microbiology laboratories affiliated with american cf patient care centers. selective media, an exotoxin ... | 2001 | 11682511 |
| in vitro susceptibility of stenotrophomonas maltophilia to various antimicrobial combinations. | stenotrophomonas maltophilia has emerged as a significant pathogen in compromised patients, causing infections which are difficult to treat. clinical isolates from patients in the tucson area were tested against single and combination antibiotics using three testing methods. ticarcillin/clavulanate, trimethoprim/sulfamethoxazole and trovafloxacin provided comparable inhibitory activity, in vitro. ciprofloxacin, imipenem and ticarcillin were active less often. agreements between disk diffusion an ... | 2001 | 11687317 |
| infection and mucosal injury in cancer treatment. | the oral and gastrointestinal mucosa acts as an important mechanical barrier that prevents local or systemic invasion by microorganisms. cytotoxic chemotherapy-induced mucosal injury (mi) of oral cavity and intestinal epithelium occurs in many patients treated for malignancy. compromise of the mucosal barrier can contribute to local invasion by colonizing microorganisms and, subsequently, to systemic infection. historically, gram-negative bacteremia has been the most problematic bacterial infect ... | 2001 | 11694563 |
| [an update on the susceptibility of stenotrophomonas maltophilia]. | 2001 | 11704768 | |
| smedef multidrug efflux pump contributes to intrinsic multidrug resistance in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an emerging nosocomial pathogen that displays high-level intrinsic resistance to a variety of structurally unrelated antimicrobial agents. efflux mechanisms are known to contribute to acquired multidrug resistance in this organism, and indeed, one such multidrug efflux system, smedef, was recently identified. still, the importance of smedef to intrinsic antibiotic resistance in s. maltophilia had not yet been determined. reverse transcription-pcr confirmed express ... | 2001 | 11709330 |
| biclonal bacteremia due to stenotrophomonas maltophilia in a cancer patient. | 2001 | 11714054 | |
| management of an older patient with stenotrophomonas maltophilia: is it an emerging pathogen in cystic fibrosis? | 2001 | 11720067 | |
| identification of pandoraea species by 16s ribosomal dna-based pcr assays. | the recently described genus pandoraea contains five named species (pandoraea apista, pandoraea pulmonicola, pandoraea pnomenusa, pandoraea sputorum, and pandoraea norimbergensis) and four unnamed genomospecies. pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (cf) patients. accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from burkholderia cepacia complex organisms may be especiall ... | 2001 | 11724860 |
| microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry. | the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ... | 2001 | 11873883 |
| comparative in vitro potency of gemifloxacin and fluoroquinolones against recent european clinical isolates from a global surveillance study. | gemifloxacin, a new fluoroquinolone with enhanced activity against gram-positive aerobes, was compared to ciprofloxacin, levofloxacin and ofloxacin against 21,464 recent isolates from 16 european countries. gemifloxacin was the most potent fluoroquinolone against streptococci including penicillin-, macrolide- and ciprofloxacin-resistant streptococcus pneumoniae, staphylococcus aureus, coagulase-negative staphylococci, acinetobacter spp., haemophilus spp. and moraxella catarrhalis. this drug was ... | 2001 | 11783700 |
| assessment of chitin decomposer diversity within an upland grassland. | the breakdown of chitin within an acidic upland grassland was studied. the aim was to provide a molecular characterisation of microorganisms involved in chitin degradation in the soil using soil microcosms and buried litter bags containing chitin. the investigation involved an examination of the effects of liming on the microbial communities within the soil and their chitinolytic activity. microcosm experiments were designed to study the influence of lime and chitin enrichment on the grassland s ... | 2001 | 11816968 |
| microflora investigation experiment. | many microorganisms were isolated from condensed water, wiping and scratching of cabin wall and air sampler in russian space station mir by russian astronauts lazutkin et al. in february 1997 as part of nasda "first mir utilization space experiment (jmir)". for example, there were about 2 x 10(6) cells/ml in condensed water sample no. 1 isolated from the transfer-docking compartment of crystal module. we tried the colony isolation, pure culturing and identification from these sampled microorgani ... | 2001 | 11799257 |
| post-traumatic endophthalmitis caused by xanthomonas maltophilia. | 2001 | 11827012 | |
| [stenotrophomonas maltophilia as a cause of hospital infections--typing of clinical isolates using pulsed field gel electrophoresis] ]. | stenotrophomonas maltophilia (s. maltophilia) has been recognised now as an important cause of hospital infections. as s. maltophilia is resistant to many antibiotics, attempts have been made to identify the sources of s. maltophilia infection and route of its transmission. from july till october 1998, 22 isolates of s. maltophilia were obtained from 20 patients hospitalised at eight different wards. strain typing was performed by macrorestriction analysis of chromosomal dna by use of pfge (xbai ... | 2001 | 11845578 |
| analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (dgge) of 16s rdna based pcr fragments. | the diversity of endophytic bacterial populations of potato (solanum tuberosum cv desirée) was assessed using a combination of dilution plating of plant macerates followed by isolation and characterization of isolates, and direct pcr-dgge on the basis of dna extracted from plants. the culturable endophytic bacterial communities detected in potato stem bases as well as in roots were in most cases on the order 103 to 105 cfu g?1 of fresh plant tissue. dilution plating revealed that a range of bact ... | 2001 | 12032611 |
| [study on molecular epidemiology of stenotrophomonas maltophilia in intensive care units]. | to investigate the epidemiological conditions of stenotrophomonas maltophilia (s. maltophilia) and their origins in intensive care units (icu). | 2001 | 16136901 |
| liver abscess caused by stenotrophomonas (xanthomonas) maltophilia in a patient with aids. | 2001 | 11774837 | |
| influence of temperature and inoculum density of fusarium oxysporum f. sp. ciceris on suppression of fusarium wilt of chickpea by rhizosphere bacteria. | the effects of temperature and inoculum density of fusarium oxysporum f. sp. ciceris race 5 on suppression of fusarium wilt in chickpea (cicer arietinum) cv. pv 61 by seed and soil treatments with rhizobacteria isolated from the chickpea rhizosphere were studied in a model system. disease development over a range of temperatures (20, 25, and 30 degrees c) and inoculum densities (25 to 1,000 chlamydospores per gram of soil) was described by the gompertz model. the gompertz relative rate of diseas ... | 2001 | 18944039 |
| chitinases from the plant disease biocontrol agent, stenotrophomonas maltophilia c3. | abstract stenotrophomonas maltophilia strain c3, a biocontrol agent of bipolaris sorokiniana in turfgrass, produced chitinases in broth media containing chitin. chitinases were partially purified from culture fluid by ammonium sulfate precipitation and chitin affinity chromatography. the chromatography fraction with the highest specific chitinase activity was inhibitory to conidial germination and germ-tube elongation of b. sorokiniana, but it was less inhibitory than the protein fraction or the ... | 2001 | 18944395 |
| characterization of a new keratin-degrading bacterium isolated from deer fur. | a keratin-degrading bacterium was isolated from soil containing deer fur. an axenic culture of the keratin-degrading bacterium was obtained in liquid culture using a keratin enrichment technique. the isolated bacterium was gram negative and catalase- and oxidase-positive. transmission electron microscopic observations showed that the bacterium was rod-shaped, 1.0-1.3 microm long and 0.7 microm in diameter. phylogenetic analysis of 16s rdna revealed that the new isolate has only 90.6% homology wi ... | 2002 | 16233255 |
| [second multicenter study of the in vitro susceptibility to piperacillin-tazobactam]. | a study was conducted in 1999 on the activity of piperacillin-tazobactam on 1349 strains collected in a spanish multicenter study involving 10 hospitals. a total of 897 gram-negative bacteria of the following genuses were collected: escherichia coli (169), klebsiella spp. (112), pseudomonas spp. (122), enterobacter spp. (92), serratia spp. (44), citrobacter spp. (37), proteus spp. (114), morganella spp. (49), acinetobacter spp. (47), stenotrophomonas spp. (47), haemophilus spp. (45), moraxella s ... | 2002 | 12582446 |
| nosocomial stenotrophomonas maltophilia strain with a broad host range ability to transfer antibiotic resistance. | 2002 | 12583560 | |
| meningitis caused by stenotrophomonas maltophilia: case report and review of the literature. | 2002 | 17159399 | |
| attributable mortality of stenotrophomonas maltophilia bacteremia. | a systematic evaluation of the attributable mortality of stenotrophomonas maltophilia bacteremia was undertaken in a matched, retrospective, case-control study. we determined the attributable mortality rate (26.7%) and mortality risk ratio (an 8-fold increase) of s. maltophilia bacteremia. the attributable mortality rate for s. maltophilia bacteremia is similar to the attributable mortality rate for other nosocomial bloodstream infections. | 2002 | 12032905 |
| heterogeneity of dehydrogenases of stenotrophomonas maltophilia showing dye-linked activity with polypropylene glycols. | distinct enzyme activities were found in extracts from stenotrophomonas maltophilia showing dye-linked oxidation of polypropylene glycols. the activities were induced when polypropylene glycols served as sole carbon and energy sources for the bacterium. in the logarithmic phase, most of the enzyme activities (88%) were found in the cytoplasm. in the stationary phase, more than half of the activities (54%) were found on the membrane, but significant activities were also distributed in the peripla ... | 2002 | 12036044 |
| prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
| characterization of cholylglycine hydrolase from a bile-adapted strain of xanthomonas maltophilia and its application for quantitative hydrolysis of conjugated bile salts. | purified bile salt hydrolase from bile-adapted xanthomonas maltophilia displays michaelis-menten kinetics on cholylglycine and cholyltaurine and hydrolyzes bile salts also in crude bovine bile. the protein is a dimer and is resistant to proteinases and to heating at 55 to 60 degrees c for up to 60 min, in agreement with calorimetric data. | 2002 | 12039776 |
| microbiota of periapical lesions refractory to endodontic therapy. | the periapical microbiota of 36 teeth with refractory apical periodontitis was investigated. none of the teeth had responded to conventional endodontic or long-term (> 6 months), calcium-hydroxide treatment. eight patients had received antibiotics systemically. after anaerobic culture, a total of 148 microbial strains were detected among 67 microbial species. one of the 36 lesions was culture-negative. approximately half (51.0%) of the bacterial strains were anaerobic. gram-positive species cons ... | 2002 | 12043869 |
| factors affecting the incidence of stenotrophomonas maltophilia isolation in cystic fibrosis. | to identify factors predisposing cystic fibrosis (cf) patients to stenotrophomonas maltophilia infection and to determine whether coinfection with s maltophilia affects the clinical response to therapy with tobramycin solution for inhalation (tsi), 300 mg bid. | 2002 | 12065335 |
| stenotrophomonas maltophilia meningitis, bacteremia and respiratory infection. | stenotrophomonas maltophilia is an increasingly recognized cause of nosocomial infection of special interest because of its resistance to multiple antimicrobial agents. we report a case of generalized infection by s. maltophilia, including meningitis, bacteremia and respiratory tract infection, in a patient who had undergone multiple neurosurgical procedures and who was treated with trimethoprim-sulphamethoxazole. | 2002 | 12069028 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ... | 2002 | 12071094 |
| metabolite repression inhibits degradation of benzo[a]pyrene and dibenz[a,h]anthracene by stenotrophomonas maltophilia vun 10,003. | large inocula of stenotrophomonas maltophilia vun 10,003 were used to investigate bacterial degradation of benzo[a]pyrene and dibenz[a,h]anthracene. although strain vun 10,003 was capable of degrading 10-15 mg (-1) of the five-ring compounds in the presence of pyrene after 63 days, further addition of pyrene after degradation of the five-ring polycyclic aromatic hydrocarbons (pahs) ceased did not stimulate significant decreases in the concentration of benzo[a]pyrene or dibenz[a,h]anthracene. how ... | 2002 | 12074057 |
| keratin degradation: a cooperative action of two enzymes from stenotrophomonas sp. | a novel keratin-degrading bacterium stenotrophomonas sp. strain d-1, isolated from deer fur, produced two types of extracellular proteins: proteolytic and disulfide bond-reducing. the results on the biochemical properties suggest that this protease belongs to the serine protease, and the disulfide bond-reducing protein could be the disulfide reductase type. none of these enzymes showed keratinolytic activity independently. however, after mixing of the two enzymes, the keratinolytic activity was ... | 2002 | 12074595 |
| comparative antimicrobial spectrum and activity of the desfluoroquinolone bms284756 (t-3811) tested against non-fermentative gram-negative bacilli. | to compare the in vitro activity of bms284756, a desfluoroquinolone, with four fluoroquinolones against 129 pseudomonas aeruginosa strains, 97 stenotrophomonas/burkholderia group strains and 43 acinetobacter spp. strains by three in vitro test methods. | 2002 | 12084101 |
| [interpretative reading of the non-fermenting gram-negative bacilli antibiogram]. | among non-fermenting gram-negative rods, the most clinically important species are pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, which are frequently multiresistant. p. aeruginosa resistance to beta-lactams depends on the production of chromosomal and plasmid-mediated beta-lactamases, altered permeability (loss of oprd porin is related to carbapenem-resistance) and active efflux pumps, particularly mexab-oprm. in aminoglycoside-resistant strains the main mech ... | 2002 | 12084355 |
| highly antibacterial active aminoacyl penicillin conjugates with acylated bis-catecholate siderophores based on secondary diamino acids and related compounds. | new acylated bis-catecholates and 1,3-benzoxazine-2,4-dione derivatives based on secondary diamino acids (n-(aminoalkyl)glycines, n-aminopropyl-alanine, and n-aminopropyl-4-aminovaleric acid), on n-(aminoalkyl)aminomethyl benzoic acids, on n-(aminoalkyl)aminomethyl phenoxyacetic acids, or on 3,5-diaminobenzoic acid were synthesized as artificial siderophores. the corresponding diamino acids were obtained from the diamines and oxocarboxylic acids by catalytic hydrogenation. the acylated bis-catec ... | 2002 | 12086488 |
| antimicrobial multiresistance in bacteria isolated from freshwater chilean salmon farms. | the intensive use of antimicrobial agents, mainly oxytetracycline, to prevent and control bacterial pathologies in chilean salmon culture is a frequent practice. a total of 103 gram-negative oxytetracycline-resistant bacteria recovered from various sources of 4 chilean freshwater salmon farms were identified and investigated for their susceptibility patterns to various antibacterial agents, by using an agar disk diffusion method. antibacterial resistance patterns of isolates were not correlated ... | 2002 | 12109474 |
| risk factors for late-onset nosocomial pneumonia caused by stenotrophomonas maltophilia in critically ill trauma patients. | patients with nosocomial pneumonia caused by stenotrophomonas maltophilia often receive inadequate empiric antibiotic therapy, potentially increasing mortality. knowledge of the risk factors associated with s. maltophilia pneumonia may better guide the selection of empiric antibiotic therapy. potential risk factors for s. maltophilia pneumonia were retrospectively analyzed for critically ill trauma patients with late-onset gram-negative pneumonia. the effects of s. maltophilia infection and inad ... | 2002 | 12115086 |
| synergistic activity of the novel des-fluoro(6) quinolone, garenoxacin (bms-284756), in combination with other antimicrobial agents against pseudomonas aeruginosa and related species. | non-fermentative gram-negative bacteria (pseudomonas aeruginosa, burkholderia cepacia, stenotrophomonas maltophilia and acinetobacter spp.) are intrinsically less susceptible to many antimicrobial agents. two-drug combinations have been used to treat infections caused by less susceptible pathogens. in this study, the antibacterial activity of garenoxacin (garx) with non-quinolones was examined. the non-quinolones evaluated were cefepime (cepi), imipenem (imip), aztreonam (aztr), piperacillin-taz ... | 2002 | 12127712 |
| co-infection of the human cornea with stenotrophomonas maltophilia and aspergillus fumigatus. | to report a case of corneal co-infection of stenotrophomonas maltophilia and aspergillus fumigatus. | 2002 | 12131049 |
| synthesis, antiprotozoal and antibacterial activity of nitro- and halogeno-substituted benzimidazole derivatives. | two series of benzimidazole derivatives were sythesised. the first one was based on 5,6-dinitrobenzimidazole, the second one comprises 2-thioalkyl- and thioaryl-substituted modified benzimidazoles. antibacterial and antiprotozoal activity of the newly obtained compounds was studied. some thioalkyl derivatives showed remarkable activity against nosocomial strains of stenotrophomonas malthophilia, and an activity comparable to that of metronidazole against gram-positive and gram-negative bacteria. ... | 2002 | 12136939 |
| comparative in vitro activity of levofloxacin and ciprofloxacin against bacterial isolates from neutropenic patients. | novel fluoroquinolones have been recently introduced in the management of neutropenic patients because of their increased activity against gram-positive and gram-negative micro-organisms. | 2002 | 12138329 |
| isolation of s-n-butylcysteine sulfoxide and six n-butyl-containing thiosulfinates from allium siculum. | isolation and identification of (s(s)r(c))-s-n-butylcysteine sulfoxide (1) from the bulbs of allium siculum is reported. this compound was found in all parts of the plant (bulbs, stem, leaves, and flowers) along with known compounds (s(s)r(c))-s-methyl- and (r(s)r(c))-(e)-s-(1-propenyl)cysteine sulfoxides (2 and 3, respectively). in addition, six n-butyl-containing thiosulfinates (4-9) have been found in a ch2cl2 extract of the bulbs. structures were determined by a combination of spectral metho ... | 2002 | 12141853 |
| evaluation of a new etest for detecting metallo-beta-lactamases in routine clinical testing. | several etest (ab biodisk, solna, sweden) gradient formats were developed for detection of metallo-beta-lactamases based on the reduction of imipenem (ip) or ceftazidime (tz) mics in the presence of edta or 2-mercaptopropionic acid (mpa). the etest metallo-beta-lactamase (etest mbl) strips consisted of a double-sided seven-dilution range of ip or tz (4 to 256 microg/ml) and ip or tz (1 to 64 microg/ml) overlaid with a constant concentration of edta or mpa. the prototype strips were evaluated on ... | 2002 | 12149325 |
| detecting stenotrophomonas maltophilia does not reduce survival of patients with cystic fibrosis. | stenotrophomonas maltophilia is a gram-negative bacterium that has been cultured with increasing prevalence from the sputum of patients with cystic fibrosis (cf). we conducted a cohort study, using the cystic fibrosis foundation registry, to assess the effect of s. maltophilia on survival. we studied all patients in this registry from 1991 to 1997 who were older than 6 years of age, were s. maltophilia negative in the first year of enrollment, and had their cf diagnosed before the age of 45 year ... | 2002 | 12153970 |
| quantitative culture of endotracheal aspirates in the diagnosis of ventilator-associated pneumonia in patients with treatment failure. | to study the correlation of bacteriology between quantitative cultures of protected specimen brush (psb), bal, and quantitative endotracheal aspirate (qea) in ventilator-associated pneumonia (vap) patients with treatment failure. | 2002 | 12171848 |
| identification and antimicrobial susceptibility testing of clinical isolates of nonfermenting gram-negative bacteria by the phoenix automated microbiology system. | the phoenix automated microbiology system (becton dickinson, sparks, md) was evaluated for its ability to identify nonfermenting gram-negative pathogens and measure their drug susceptibility. isolates producing rare extended-spectrum beta-lactamases (per-1, imp-2, vim-1, and vim-2) were included in the study. species identification was compared to that given by the atb system (bio-mérieux, marcy l'etoile, france), whereas susceptibility results were compared to those produced by a reference brot ... | 2002 | 12173774 |
| [occurrence of beta-lactamase types esbl and ampc among gram-negative rods isolated from food]. | the aim of the study was to isolate extended spectrum beta-laktamases (esbl) and chromosomal beta-laktamases ampc producing strains from food products. a total of 739 gram-negative bacteria were tested with double disc diffusion method using cefotaxim, ceftasidim and amoxycillin with clavulanic acid. one strain producing esbl and belonging to stenotrophomonas maltophilia was detected from the ice cream (0.14% of all strains). from different food products a total of 14 microorganisms (1.9%) havin ... | 2002 | 12185694 |
| characterization of flagella produced by clinical strains of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an emerging nosocomial pathogen associated with opportunistic infections in patients with cystic fibrosis, cancer, and hiv. adherence of this organism to abiotic surfaces such as medical implants and catheters represents a major risk for hospitalized patients. the adhesive surface factors involved in adherence of these bacteria are largely unknown, and their flagella have not yet been characterized biochemically and antigenically. we purified and characterized the ... | 2002 | 12194767 |
| bacteraemia due to stenotrophomonas maltophilia: an analysis of 45 episodes. | stenotrophomonas maltophilia is an important nosocomial pathogen and a therapeutic challenge. a ten-year review of episodes of bacteraemia due to s. maltophilia was undertaken in light of reports of an increasing frequency of infection. | 2002 | 12217732 |
| [plantar pyoderma due to stenotrophomonas maltophilia]. | stenotrophomonas maltophilia is a gram negative, opportunistic bacteria. we describe a case of community-acquired superficial pyoderma due to this bacteria in a immunocompetent host. | 2002 | 12218917 |
| xanthomonas maltophilia endophthalmitis following penetrating corneal injury. | 2002 | 12322863 | |
| in vitro activities of antimicrobial combinations against clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia, a major pathogen causing nosocomial infection, is inherently resistant to multiple antimicrobial agents. evaluation of the effectiveness of recommended therapeutic options for s. maltophilia infections is crucial, particularly in areas with high antimicrobial resistance in this nosocomial pathogen. | 2002 | 12353342 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| clonal diversity and metallo-beta-lactamase production in clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is a nosocomial pathogen with an intrinsic broad-spectrum resistance to beta-lactam compounds and other antibacterial agents. it produces two chromosomal beta-lactamases: a clavulanic acid-sensitive class a (l2) and a tetrameric carbapenemase (l1 or blas). we screened 40 s. maltophilia multidrug-resistant clinical isolates recovered between 1995 and 1998 in the varese hospital (italy) for the presence of the metallo-beta-lactamase. the isolates were investigated by p ... | 2002 | 12363008 |
| an alternative mechanism for amidase signature enzymes. | the peptide amidase from stenotrophomonas maltophilia catalyses predominantly the hydrolysis of the c-terminal amide bond in peptide amides. peptide bonds or amide functions in amino acid side-chains are not hydrolysed. this specificity makes peptide amidase (pam) interesting for different biotechnological applications. pam belongs to the amidase signature (as) family. it is the first protein within this family whose tertiary structure has been solved. the structure of the native pam has been de ... | 2002 | 12367528 |
| cloning and characterization of smet, a repressor of the stenotrophomonas maltophilia multidrug efflux pump smedef. | we report on the cloning of the gene smet, which encodes the transcriptional regulator of the stenotrophomonas maltophilia efflux pump smedef. smet belongs to the tetr and acrr family of transcriptional regulators. the smet gene is located upstream from the structural operon of the pump genes smedef and is divergently transcribed from those genes. experiments with s. maltophilia and the heterologous host escherichia coli have demonstrated that smet is a transcriptional repressor. s1 nuclease map ... | 2002 | 12384340 |
| molecular epidemiology of stenotrophomonas maltophilia in a university hospital. | the aim of this investigation was to study the molecular epidemiology of stenotrophomonas maltophilia in a university hospital in italy. sixty-one clinical isolates were collected from 43 patients during a two-year period. the majority of specimens were from the respiratory tract (41 of 43) of patients in the adult intensive care unit (icu) (19 of 43) or cystic fibrosis (cf) patients (13 of 43). genotypic analysis by pulsed-field gel electrophoresis (pfge) of clinical isolates identified 31 diff ... | 2002 | 12392899 |
| comparative in vitro activities of tigecycline (gar-936) and other antimicrobial agents against stenotrophomonas maltophilia. | 2002 | 12407139 | |
| diversity of transconjugants that acquired plasmid pjp4 or pemt1 after inoculation of a donor strain in the a- and b-horizon of an agricultural soil and description of burkholderia hospita sp. nov. and burkholderia terricola sp. nov. | we examined the diversity of transconjugants that acquired the catabolic plasmids pjp4 or pemt1, which encode degradation of 2,4-dichlorophenoxyacetic acid (2,4-d), in microcosms with agricultural soil inoculated with a donor strain (dejonghe, w., goris, j., el fantroussi, s., höfte, m., de vos, p., verstraete, w., and top, e. m. appl. environ. microbiol. 2000, p. 3297-3304). using repetitive element pcr fingerprinting, eight different rep-clusters and six separate isolates could be discriminate ... | 2002 | 12421072 |
| [endocarditis due to stenotrophomonas maltophilia]. | 2002 | 12425885 | |
| in vitro killing effect of moxifloxacin on clinical isolates of stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole. | the time-kill effect of moxifloxacin on 20 genetically distinct isolates of stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole was studied. the majority (80%) were killed by a concentration equivalent to four times the mic; the mic induced a transient decrease in bacterial counts at 4 h, followed by regrowth. no effect was detected in four isolates. these results merit further clinical consideration. | 2002 | 12435710 |
| antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients. | the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ... | 2002 | 12458127 |
| development of broad-range polymerase chain reaction (pcr) bacterial identification in diagnosis of infective endocarditis. | the study aim was to identify the value of broad-range bacterial pcr in infective endocarditis (ie) of bacterial etiology, and to determine its specificity and sensitivity. | 2002 | 12479291 |
| synergy of colistin with rifampin and trimethoprim/sulfamethoxazole on multidrug-resistant stenotrophomonas maltophilia. | stenotrophomonas maltophilia is characterized by intrinsic resistance to a variety of antimicrobials. therapeutic options are often limited particularly after the emergence of isolates resistant to trimethoprim/sulfamethoxazole. the application of colistin for infections caused by multidrug-resistant gram-negative pathogens is limited due to its toxicity. in order to evaluate the activity of the interaction of colistin with rifampin or trimethoprim/sulfamethoxazole on s. maltophilia, 24 differen ... | 2002 | 12493173 |
| functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements. | at present there are three protein families that share a common structural domain, the alphabeta/betaalpha fold of class b beta-lactamases: zinc beta-lactamases, glyoxalases ii, and a-type flavoproteins. a detailed inspection of their superimposed structures was undertaken and showed that although these proteins contain binuclear metal sites in spatially equivalent positions, there are some subtle differences within the first ligand sphere that determine a distinct composition of metals. althoug ... | 2002 | 11847294 |
| topoisomerase ii and iv quinolone resistance-determining regions in stenotrophomonas maltophilia clinical isolates with different levels of quinolone susceptibility. | the quinolone resistance-determining regions (qrdrs) of topoisomerase ii and iv genes from stenotrophomonas maltophilia atcc 13637 were sequenced and compared with the corresponding regions of 32 unrelated s. maltophilia clinical strains for which ciprofloxacin mics ranged from 0.1 to 64 microg/ml. gyra (leu-55 to gln-155, escherichia coli numbering), gyrb (met-391 to phe-513), parc (ile-34 to arg-124), and pare (leu-396 to leu-567) fragments from strain atcc 13637 showed high degrees of identit ... | 2002 | 11850246 |
| in vitro activities of tigecycline (gar-936) against recently isolated clinical bacteria in spain. | the antimicrobial activities of tigecycline (gar-936) were compared with those of other agents against 1,087 strains recently isolated in 12 spanish medical centers. tigecycline showed activity against a wide spectrum of aerobic and anaerobic bacteria, including strains such as methicillin-resistant staphylococcus aureus, coagulase-negative staphylococci, penicillin-resistant streptococcus pneumoniae, enterococcus faecium, acinetobacter baumannii, and stenotrophomonas maltophilia. | 2002 | 11850282 |
| in vitro activity of clinafloxacin in comparison with other quinolones against stenotrophomonas maltophilia clinical isolates in the presence and absence of reserpine. | a total of 33 stenotrophomonas maltophilia clinical isolates were tested for their susceptibility to clinafloxacin in comparison with ciprofloxacin, levofloxacin, moxifloxacin, nalidixic acid, norfloxacin, sparfloxacin and trovafloxacin. the mic(50) and mic(90) were as follows: ciprofloxacin 4 and 64 microg/ml; clinafoxacin 0.5 and 4 microg/ml; levofloxacin 2 and 32 microg/ml; moxifloxacin 1 and 8 microg/ml; nalidixic acid 8 and 128 microg/ml; norfloxacin 64 and 256 microg/ml; sparfloxacin 1 and ... | 2002 | 11858908 |
| stenotrophomonas maltophilia endocarditis: a systematic review. | the disease characteristics, management, and outcome of stenotrophomonas maltophilia endocarditis were evaluated by examining the reports on the subject identified through a comprehensive literature search. twenty-three (17 male) cases of s.. maltophilia endocarditis were identified. mean age was 41 +/- 15 years. all patients presented with fever. prosthetic valves were involved in 12 (52%) cases. among native valves, the aortic valve was most frequently involved (50%), followed by the tricuspid ... | 2002 | 11863309 |
| characterization of a chitinase gene from stenotrophomonas maltophilia strain 34s1 and its involvement in biological control. | a chitinase gene was cloned on a 2.8-kb dna fragment from stenotrophomonas maltophilia strain 34s1 by heterologous expression in burkholderia cepacia. sequence analysis of this fragment identified an open reading frame encoding a deduced protein of 700 amino acids. removal of the signal peptide sequence resulted in a predicted protein that was 68 kda in size. analysis of the sequence indicated that the chitinase contained a catalytic domain belonging to family 18 of glycosyl hydrolases. three pu ... | 2002 | 11872449 |
| microscale biosensor for measurement of volatile fatty acids in anoxic environments. | a microscale biosensor for acetate, propionate, isobutyrate, and lactate is described. the sensor is based on the bacterial respiration of low-molecular-weight, negatively charged species with a concomitant reduction of no(-)(3) to n(2)o. a culture of denitrifying bacteria deficient in n(2)o reductase was immobilized in front of the tip of an electrochemical n(2)o microsensor. the bacteria were separated from the outside environment by an ion-permeable membrane and supplied with nutrients (excep ... | 2002 | 11872469 |
| stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. | a polyphasic taxonomic study was performed on 16 stenotrophomonas strains from environmental and clinical sources. a group of three plant-associated isolates were shown to be phenotypically different from the other strains. this group formed a separate physiological cluster (b1) with 42% heterogeneity to the other isolates. the defining characteristics of the new species were as follows: growth at 4 degrees c and the absence of growth at 40 degrees c; the utilization of xylose as a carbon source ... | 2002 | 12508851 |
| [microbiology and cystic fibrosis]. | the respiratory infections are the rule in sick persons of cystic fibrosis. after the colonization in one's early childhood by staphylococcus aureus and haemophilus influenzae, it is established the colonization produced by the mucous strains of pseudomonas aeruginosa, the principal etiogenic of this illness, that can become to isolate in more than 95% of sick persons at three years old. that's why the etiogenic factors of this bacterium are studied, as they are toxins, pigments, enzymes, and pr ... | 2002 | 12518659 |
| mutations in gyra and parc qrdrs are not relevant for quinolone resistance in epidemiological unrelated stenotrophomonas maltophilia clinical isolates. | clinical strains of stenotrophomonas maltophilia are often highly resistant to multiple antibiotics and this resistance is steadily rising. quinolones are included in the group of antimicrobial agents to which this microorganism is developing resistance. therefore, the aim of this study was to analyze the epidemiological relationship among 22 clinical isolates of s. maltophilia as well as the molecular mechanisms responsible for the acquisition of quinolone-resistance in these strains. the resul ... | 2002 | 12523620 |
| microbial degradation of polyethers. | this paper summarizes studies on microbial degradation of polyethers. polyethers are aerobically metabolized through common mechanisms (oxidation of terminal alcohol groups followed by terminal ether cleavage), well-characterized examples being found with polyethylene glycol (peg). first the polymer is oxidized to carboxylated peg by alcohol and aldehyde dehydrogenases and then the terminal ether bond is cleaved to yield the depolymerized peg by one glycol unit. most probably peg is anaerobicall ... | 2002 | 11831473 |
| crystallization and preliminary x-ray data of the recombinant peptide amidase from stenotrophomonas maltophilia. | the peptide amidase from stenotrophomonas maltophilia selectively hydrolyses the c-terminal amide bond in peptide amides. crystals have been obtained by sitting-drop vapour diffusion from solution containing polyethylene glycol (peg) 6000, hepes ph 7.5, glycerine and sodium azide (nan(3)). the crystals belong to the monoclinic space group p2(1), with unit-cell parameters a = 74.18, b = 62.60, c = 101.91 a, beta = 90 degrees. x-ray data from these crystals diffracted at the european synchrotron r ... | 2002 | 11807268 |