Publications

active site structure of rieske-type proteins: electron nuclear double resonance studies of isotopically labeled phthalate dioxygenase from pseudomonas cepacia and rieske protein from rhodobacter capsulatus and molecular modeling studies of a rieske center.	continuous wave electron nuclear double resonance (cw endor) spectra of [delta-15n,epsilon(-14)n]histidine-labeled phthalate dioxygenase (pdo) from pseudomonas cepacia were recorded and found to be virtually identical to those previously recorded from [delta,epsilon-15n2]histidine-labeled protein [gurbiel, r. j., batie, c. j., sivaraja, m., true, a. e., fee, j. a., hoffman, b. m., & ballou, d. p. (1989) biochemistry 28, 4861-4871]. thus, the two histidine residues, previously shown to ligate one ...	1996	8672484
melioidosis in india.		1996	8684144
in-vitro activity of carbapenem antibiotics against beta-lactam susceptible and resistant strains of burkholderia pseudomallei.	four carbapenem antibiotics were tested for their in-vitro activities (mics, mbcs and time-kill studies) against burkholderia pseudomallei. the carbapenems were all more active than either ceftazidime or co-amoxiclav against strains of b. pseudomallei with a normal susceptibility pattern. biapenem was the most active antibiotic tested. all four carbapenems retained bactericidal activity against 24 strains of b. pseudomallei with reduced susceptibility to ceftazidime and/or co-amoxiclav.	1996	9182118
affinity and response of burkholderia pseudomallei and burkholderia cepacia to insulin.	the cells of burkholderia pseudomallei, b. cepacia and pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. the former two species were stained positively indicating insulin binding but p. aeruginosa was not. insulin exposure reduced phospholipase c and acid phosphatase activities of b. pseudomallei but did not affect those enzymatic activities of b. cepacia in the employed experimental conditions. it is suggested that b. pseudomallei have insulin receptor ...	1996	9185275
evolution of cell-surface acid phosphatase of burkholderia pseudomallei.	acid phosphatase active fractions were obtained from cell-free extract, outermembrane fraction and culture filtrate of burkholderia pseudomallei by column chromatography with sepharose 6b and deae cellulose. the comparison of the elution patterns of protein, sugar and enzymatic activity among these three components suggested that the enzyme is a glycoprotein evolving from premature proteins through glycosylation and that the enzyme is translocated during glycosylation from the cytoplasm to the o ...	1996	9185276
indirect hemagglutination antibodies against burkholderia pseudomallei in normal blood donors and suspected cases of melioidosis in malaysia.	interpretation of the indirect hemagglutination test (iha) for melioidosis in endemic areas is difficult because of the presence of antibodies in apparently healthy individuals. fifty-three out of 200 healthy blood donors in malaysia showed positive antibody titers (> or = 1 : 40) against burkholderia pseudomallei. seven percent had an iha titer of 1 : 40, 11% had an iha titer of 1 : 80 while 8.5% had a titer > or = 1 : 160. out of 258 sera sent for melioidosis serology, 7% of the patients had a ...	1996	9279987
meliodosis in india.		1996	9282627
burkholderia cepacia: medical, taxonomic and ecological issues.	the increasing challenge posed by multiresistant saprophytes in medical microbiology is strikingly demonstrated by the emergence of burkholderia (formerly pseudomonas) cepacia as an opportunist pathogen in immunocompromised patients, particularly individuals with chronic granulomatous disease and cystic fibrosis (cf). best known previously as a phytopathogen and the cause of soft rot of onions, b. cepacia presents three major problems for the cf community: innate multiresistance to antimicrobial ...	1996	8958242
biochemical characteristics of clinical and environmental isolates of burkholderia pseudomallei.	the biochemical characteristics of 213 isolates of burkholderia pseudomallei from patients with melioidosis and 140 isolates from the soil in central and northeastern thailand were compared. whereas the biochemical profiles of all the clinical isolates were similar, all soil isolates from the central area and 25% of isolates from northeastern thailand comprised a different phenotype. this was characterised by the ability to assimilate l-arabinose (100%), adonitol (100%), 5-keto-gluconate (90%) a ...	1996	8958243
rp4::mu3a-mediated in vivo cloning and transfer of a chlorobiphenyl catabolic pathway.	chromosomal dna fragments encoding the ability to utilize biphenyl as sole carbon source (bph+) were mobilized by means of plasmid rp4::mu3a from strain jb1 (tentatively identified as burkholderia sp.) to alcaligenes eutrophus ch34 at a frequency of 10(-3) per transferred plasmid. the mobilized dna integrated into the recipient chromosome or was recovered as catabolic prime plasmids. three bph+ prime plasmids were transferred from a. eutrophus to escherichia coli and back to a. eutrophus without ...	1996	8969525
comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria.	the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ...	1996	8975612
disseminated melioidosis.		1996	8979590
production of specific monoclonal antibodies to burkholderia pseudomallei and their diagnostic application.	hybridomas secreting monoclonal antibodies (mabs) specific to burkholderia pseudomallei were produced by immunizing balb/cj mice with crude culture filtrate of b. pseudomallei. two monoclonal antibodies were found to be highly specific to b.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from b. pseudomallei, b. cepacia, pseudomonas aeruginosa, p.putida, and escherichia coli. one of the specific mabs, clone sp-m ...	1996	8980799
bacterial colonization as a potential source of nosocomial respiratory infections in two types of spirometer.	the potential risk of spirometers in the transmission of respiratory infections has not been yet established. we performed a prospective cross-sectional study to determine the rate of colonization of a water-sealed spirometer and a pneumotachograph, and the potential risk of cross-transmission of microorganisms to patients using each of these devices. fifty four patients (aged 51 +/- 18 (mean +/- sd) yrs) were included in the study. all of them had undergone forced spirometry with bronchodilator ...	1996	8980977
is burkholderia (pseudomonas) cepacia disseminated from cystic fibrosis patients during physiotherapy?	burkholderia cepacia is well recognized as a potential respiratory pathogen in cystic fibrosis (cf) patients and there is increasing concern about nosocomial acquisition. environmental contamination with b. cepacia before, during and after physiotherapy was studied in eight adult cf patients. air was sampled using a surface air sampler, and horizontal surfaces and pillows were sampled with moistened swabs and contact plates, respectively. thirty-nine (40%) of 97 air samples were positive and cou ...	1996	8904368
bone graft contamination from a water de-ionizer during processing in a bone bank.	an organism resembling burkholderia (formerly pseudomonas) cepacia was isolated from cultures taken during processing of six bone grafts. the source of the contamination was traced to a water de-ionizer; the problem had begun when procedures had been changed, so that water from the de-ionizer had been used without sterilization. cultures of bone for grafting not only protect recipients from infection, but act also as useful controls for processes involved in bone banking.	1996	8904374
home-use nebulizers: a potential primary source of burkholderia cepacia and other colistin-resistant, gram-negative bacteria in patients with cystic fibrosis.	inhalation of aerosols contaminated with gram-negative bacteria generated from home-use nebulizers used by cystic fibrosis (cf) patients may be a primary route for bacterial colonization of the lung. burkholderia cepacia was isolated from 3 of [corrected] 35 home-use nebulizers, and stenotrophomonas maltophilia was isolated from 4 of 35 home-use nebulizers. sputum cultures for two patients whose nebulizers were contaminated with b. cepacia did not yield the organism. however, dna macrorestrictio ...	1996	8904419
detection of burkholderia pseudomallei dna in patients with septicemic melioidosis.	septicemic melioidosis is the most severe form of melioidosis, which is caused by burkholderia pseudomallei. it is endemic in southeast asia and is the leading cause of death from community-acquired septicemia in northeast thailand. a major factor that contributes to the high mortality is the delay in isolation and identification of the causative organism. more than half of the patients die within the first 2 days after hospital admission, before bacterial cultures become positive. the present s ...	1996	8904424
bronchiolitis obliterans organizing pneumonia (boop) in lung transplant recipients.	we reviewed all tissue specimens from 163 transplant patients (108 double lung transplant [dlt], 55 single lung transplant [slt]) between november 1983 and january 1994 for abnormalities indicating bronchiolitis obliterans organizing pneumonia (boop) and found 17 cases (14 dlt and 3 slt). of the three slts, boop was diagnosed by open lung biopsy (olb) in two and one was found at autopsy. of the 14 dlts, boop was diagnosed by transbronchial biopsy (tbb) specimens (9), olb specimens (2), autopsy ( ...	1996	8915212
identification and nucleotide sequence of rhizobium meliloti insertion sequence isrm6, a small transposable element that belongs to the is3 family.	the insertion sequence isrm6 is a small transposable element identified in rhizobium meliloti strain gr4 by sequence analysis. two copies of this is element were found in strain gr4, one of them is linked to the nfe genes located on plasmid prmegr4b. isrm6 seems to be widespread in r. meliloti. data suggest that isrm6 is active in transposition at an estimated frequency of 2 x 10(-5) per generation per cell in strain gr4. this 1269-bp element carries 27/26-bp terminal imperfect inverted repeats ...	1996	8917074
burkholderia cepacia respiratory tract acquisition: epidemiology and molecular characterization of a large nosocomial outbreak.	in 1994 we investigated a large outbreak of burkholderia (formerly pseudomonas) cepacia respiratory tract acquisition. a case patient was defined as any patient with at least one sputum culture from which b. cepacia was isolated from 1 january to 31 december 1994. seventy cases were identified. most (40 [61%]) occurred between 1 february and 31 march 1994; of these, 35 (86%) were mechanically ventilated patients, 30 of whom were in an intensive-care unit (icu) when b. cepacia was first isolated. ...	1996	8666075
purification and characterization of chlorophenol 4-monooxygenase from burkholderia cepacia ac1100.	burkholderia (formerly pseudomonas) cepacia ac1100 mineralizes the herbicide 2,4,5-trichlorophenoxyacetate (2,4,5-t), and the first intermediate of 2,4,5-t degradation is 2,4,5-trichlorophenol. chlorophenol 4-monooxygenase activity responsible for 2,4,5-trichlorophenol degradation was detected in the cell extract. the enzyme consisted of two components separated during purification, and both were purified to more than 95% homogeneity. the reconstituted enzyme catalyzed the hydroxylation of sever ...	1996	8626333
trichloroethylene degradation and mineralization by pseudomonads and methylosinus trichosporium ob3b.	to examine the trichloroethylene (c2hcl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of c2hcl3 mineralization were evaluated for pseudomonas cepacia g4, pseudomonas cepacia g4 pr1, pseudomonas mendocina kr1, pseudomonas putida f1, and methylosinus trichosporium ob3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for c2hcl3 degradation. by varying the c2hcl3 concentration from 5 microm to 75 microm, vmax ...	1996	8920197
[simultaneous isolation of mrsa and pseudomonas aeruginosa using a novel selective and differential pmac agar].	pmac agar, a novel, selective and differential medium has been developed and was subjected for evaluation of its selective and differential capability of methicillin resistant staphylococcus aureus (mrsa) and pseudomonas aeruginosa from other bacteria such as bacillus, micrococcus, gram-negative bacteria and drug resistant ones. growth of mrsa and p. aeruginosa on pmac agar was facilitated and their colonies were easily differentiated. colonies of mrsa after 24 approximately 48 h incubation at 3 ...	1996	8921677
lack of evidence of nosocomial cross-infection by burkholderia cepacia among danish cystic fibrosis patients.	burkholderia cepacia isolates from nine of the ten danish cystic fibrosis (cf) patients known between 1975 and the present day to carry this organism were investigated. eight distinct genotypes were found with polymerase chain reaction ribotyping and pulsed-field gel electrophoresis. the results indicate that there is little patient-to-patient cross-infection with burkholderia cepacia within the danish cf population, even though the majority of patients attend the same cf clinic on a regular bas ...	1996	8922580
transformation of burkholderia pseudomallei by electroporation.	conditions were defined for the successful transformation of the human pathogen burkholderia pseudomallei 4845 by electroporation, using the incq plasmid pkt230. we have obtained frequencies of up to 8 x 10(3) transformants per microgram plasmid dna with freshly prepared electrocompetent b. pseudomallei. the method also allows for easy and reproducible production of frozen cell suspensions which can produce efficiencies up to 2.5 x 10(2) transformants per microgram of plasmid dna. kanamycin had ...	1996	8923967
emerging & re-emerging bacterial pathogens in india.	in spite of major successes against infectious diseases in the 20th century, new infectious diseases have emerged and old ones re-emerged in recent decades in different parts of the world. a brief survey of emerging and re-emerging bacterial diseases of public health importance in india is presented in this paper. plague re-appeared in two outbreaks in maharashtra and gujarat in 1994, indicating a breakdown of the public health measures that had prevented its occurrence for several decades. lept ...	1996	8926026
melioidosis in india: the tip of the iceberg?	diagnosis of melioidosis by the isolation of burkholderia pseudomallei from one or more body fluid/tissue specimens of 6 indian subjects, 5 of whom had not travelled outside india, is reported. the places of residence of these 6 and one patient previously reported, namely tripura (2), kerala (2), orissa (1), tamil nadu (1) and maharashtra (1) are therefore potentially endemic for melioidosis. b.pseudomallei closely resembles common contaminant pseudomonas sp. and are easily mis-identified in mic ...	1996	8926030
invasion of respiratory epithelial cells by burkholderia (pseudomonas) cepacia.	pulmonary infections caused by burkholderia (pseudomonas) cepacia are an important cause of morbidity and mortality in cystic fibrosis (cf) patients. several features suggestive of cellular invasion and intracellular sequestration of b. cepacia in cf are persistence of infection in the face of antibiotic therapy to which the organism demonstrates in vitro susceptibility and a propensity to cause bacteremic infections in patients with cf. epithelial cell invasion was demonstrated in vitro in a549 ...	1996	8926068
the dsbb gene product is required for protease production by burkholderia cepacia.	burkholderia cepacia kf1, isolated from a pneumonia patient, produces a 37-kda extracellular metalloprotease. a protease-deficient and lipase-proficient mutant, kft1007, was complemented by a clone having an open reading frame coding for a 170-amino-acid polypeptide which showed significant homology to escherichia coli dsbb. kft1007, a presumed dsbb mutant, also failed to show motility, and both protease secretion and motility were restored by the introduction of the cloned dsbb gene of b. cepac ...	1996	8926116
exploration of the relationship between tetrachlorohydroquinone dehalogenase and the glutathione s-transferase superfamily.	tetrachlorohydroquinone dehalogenase is found in sphingomonas chlorophenolica, a soil bacterium that degrades pentachlorophenol, a widely used wood preservative. this enzyme converts tetrachlorohydroquinone (tchq) to trichlorohydroquinone (trichq) and trichq to dichlorohydroquinone (dchq) (xun et al. (1992) j. bacteriol. 174, 8003-8007). the reducing equivalents for each step are provided by two molecules of glutathione (xun et al. (1992) biochem. biophys. res. commun. 182, 361-366). in addition ...	1996	8931562
nosocomial burkholderia cepacia outbreaks and pseudo-outbreaks.		1996	8934237
three-year outbreak of pseudobacteremia with burkholderia cepacia traced to a contaminated blood gas analyzer.	between november 1990 and june 1993, burkholderia cepacia was isolated from the blood cultures of 13 neonates born at the ottawa general hospital. eight of the 13 neonates appeared symptomatic, and only 4 were treated with appropriate antimicrobial therapy, but all improved clinically. in august 1993, the blood gas analyzer in the neonatal intensive-care unit was found to be contaminated heavily with b cepacia. eight available patient isolates were identical to the isolates recovered from the bl ...	1996	8934241
an outbreak of burkholderia cepacia lower respiratory tract infection associated with contaminated albuterol nebulization solution.	an outbreak of burkholderia cepacia lower respiratory tract colonization and infection occurred in the adult intensive-care units in various geographic locations throughout our hospital. forty-four patients became colonized or infected over an 11-month period, whereas b cepacia had been isolated from only 13 patients in the preceding 48 months. environmental cultures revealed the source to be extrinsically contaminated albuterol nebulization solution. polymerase chain reaction-ribotyping confirm ...	1996	8934242
biological activities of lipopolysaccharide of burkholderia (pseudomonas) pseudomallei.	endotoxic activities of lipopolysaccharide (lps) isolated from burkholderia (pseudomonas) pseudomallei, a causative agent of melioidosis, were investigated. compared to an enterobacterial lps (sae-lps), b. pseudomallei lps (bp-lps) exhibited weaker pyrogenic activity in rabbits, lethal toxicity in galactosamine-sensitized mice and murine macrophage activation, i.e. production of tumor necrosis factor, interleukin-6 and nitric oxide. bp-lps, on the other hand, exhibited stronger mitogenic activit ...	1996	8935661
epidemiology of burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic dna fingerprinting.	we fingerprinted a collection of 627 burkholderia cepacia isolates from 255 patients with cystic fibrosis (cf) and 43 patients without cf and from the environment, by a pcr-based randomly amplified polymorphic dna (rapd) method with primers selected for their ability to produce discriminatory polymorphisms. the rapd typing method was found to be reproducible and discriminatory, more sensitive than pcr ribotyping, and able to group epidemiologically related b. cepacia strains previously typed by ...	1996	8940422
burkholderia pseudomallei activates complement and is ingested but not killed by polymorphonuclear leukocytes.	the mechanism by which burkholderia pseudomallei is resistant to lysis by human serum is unknown but may include interference with complement activation, effective opsonization, or complement-mediated lysis. we investigated the interaction of b. pseudomallei with complement in the presence and absence of specific antibody to determine potential mechanisms of serum resistance. we demonstrated rapid activation and consumption of complement by b. pseudomallei which, in the absence of specific antib ...	1996	8945532
epidemiology of pulmonary colonization with burkholderia cepacia in cystic fibrosis patients. the french observatoire burkholderia cepacia study group.		1996	8950569
measurement of three-bond coupling constants in the osmoregulated periplasmic glucan of burkholderia solanacearum.	the cyclic osmoregulated periplasmic glucan produced by burkholderia solanacearum contains 13 glucose units, all beta-(1-2) linked except for one alpha-(1-6) linkage. we report here the measurement of the 3j(c1-h2') and 3j(h1-c2') coupling constants, characterizing the glycosidic linkages, through the use of a 13c/12c double half-filtered noesy experiments. the values obtained give information about the (phi, psi) angles of the different linkages. the results presented from an important step tow ...	1996	8953219
biodegradation of 2,4,5-trichlorophenoxyacetic acid by burkholderia cepacia strain ac1100: evolutionary insight.	many microorganisms in nature have evolved new genes which encode catabolic enzymes specific for chlorinated aromatic substrates, allowing them to utilize these compounds as sole sources of carbon and energy. an understanding of the evolutionary mechanisms involved in the acquisition of such genes may facilitate the development of microorganisms with enhanced capabilities of degrading highly chlorinated recalcitrant compounds. a number of studies have been based on microorganisms isolated from t ...	1996	8955624
molecular epidemiology of burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans in a cystic fibrosis center.	burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans have been isolated with increasing frequency from the sputum of patients with cystic fibrosis in a pediatric hospital. in 1994-95, 27 of 120 patients were persistently colonized, 17 with burkholderia cepacia, eight with alcaligenes xylosoxidans, and five with stenotrophomonas maltophilia. genotyping of 220 clinical isolates revealed that most of the burkholderia cepacia strains were clonally related, suggesting eith ...	1996	8997562
structure of the o-specific polysaccharide from burkholderia vietnamiensis strain lmg 6998.	the putative o-specific polymer containing d-mannose and l-rhamnose was isolated from the lipopolysaccharide obtained from cells walls of burkholderia vietnamiensis strain lmg 6998. nmr and degradative studies showed that the polymer has a linear trisaccharide repeating-unit of the structure shown. the same polymer carrying an o-acetyl group at position 3 of the 4-substituted mannose residue has previously been found as the o antigen in the related species burkholderia cepacia serogroup j. [form ...	1996	9002192
structural studies of the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia, isolated from cystic fibrosis.	the acidic exopolysaccharide produced by a mucoid strain of burkholderia cepacia isolated from a cystic fibrosis patient, was purified by cetyltrimethylammonium bromide precipitation and/or anion-exchange chromatography. based on the sugar composition and permethylation analyses, supported by glc-ms and nmr spectroscopy analyses, the repeating-unit of the polysaccharide was established as -->3)-beta-d-glcp-(1-->3)-[4,6-o-(1-carboxyethylidene)]-alpha-d-gal p-(1-->.	1996	9011377
polymerase chain reaction for the detection of pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia in sputum of patients with cystic fibrosis.	occurrence of pseudomonas aeruginosa, stenotrophomonas (xanthomonas) maltophilia and burkholderia (pseudomonas) cepacia in sputum of cystic fibrosis (cf) patients was demonstrated with a simple and rapid polymerase chain reaction (pcr) technique. the pcr was performed with a set of three primer pairs based on 16s rrna sequences after sputum preparation with dithiothreitol and naoh lysis. all three pathogens could be individually detected by the use of this technique. to prevent carry-over contam ...	1996	9025076
[the immunogenicity and heterogeneity of pseudomonas pseudomallei surface antigen 8].	surface antigen 8, one of pathogenicity factors of p.pseudomallei and having an antiphagocytic action, contains glycoprotein with a mol. wt. of 200 kd and proteins with mol wt. of 25, 30 and 34 kd. according to its chemical structure, the carbohydrate part of antigen 8 is the homogeneous polymer of 6-d-d-mannoheptose, and its protein component is a collection of monomer proteins with mol. wt. of 12-120 kd. the consecutive fractionation of antigen 8 by gel and ion exchange chromatography has made ...	1996	9027183
multifactorial pathogenic mechanisms of burkholderia pseudomallei as suggested from comparison with burkholderia cepacia.	with the purpose to elucidate the pathogenesis of disease due to burkholderia pseudomallei some biological and biochemical properties of this species were studied in comparison with b. cepacia, since the difference in the level of virulence between the two species is remarkable despite of their toxonomic closeness. b. pseudomallei was distinct from b. cepacia in the capability to grow under anaerobic conditions, with positive nitrate respiration, excretion of high-molecular polysaccharides into ...	1996	9031412
[microbiological parameters of clinical interest in pulmonary infection in cystic fibrosis].	a 5-year study in patients with cystic fibrosis was carried out in order to gain a better understanding of the microbiological factors influencing clinical status and evolution.	1996	9044640
the laboratory diagnosis of melioidosis.	the recognition of unusual, but important, pathogens such as burkholderia pseudomallei is essential for the rapid implementation of appropriate antimicrobial therapy--delays can be fatal. melioidosis should be considered as a potential diagnosis for any patient with exposure to areas of endemicity, and thus laboratories should be aware of the differential features of the disease and the causative organism. isolation of b. pseudomallei is readily achieved using standard culture media such as bloo ...	1996	9069100
susceptibilities of non-pseudomonas aeruginosa gram-negative nonfermentative rods to ciprofloxacin, ofloxacin, levofloxacin, d-ofloxacin, sparfloxacin, ceftazidime, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, and imipenem.	agar dilution mics of 10 agents against 410 non-pseudomonas aeruginosa gram-negative nonfermentative rods were determined. mics at which 50 and 90% of the isolates were inhibited, respectively, were as follows (in micrograms per milliliter): sparfloxacin, 0.5 and 8.0; levofloxacin, 1.0 and 8.0; ciprofloxacin, 2.0 and 32.0; ofloxacin, 2.0 and 32.0; d-ofloxacin, 32.0 and > 64.0; ceftazidime, 8.0 and 64.0; piperacillin with or without tazobactam, 16.0 and > 64.0; trimethoprim-sulfamethoxazole, 0.5 ...	1996	8851609
molecular analysis of burkholderia (pseudomonas) cepacia: differentiation between relapse and reinfection in a case of recurrent bacteremia.		1996	8852993
burkholderia (pseudomonas) cepacia and cystic fibrosis: the epidemiology in belgium.	burkholderia cepacia has become an increasingly recognized pathogen among cystic fibrosis (cf) patients and its potential role in declining pulmonary function or unexpected fatal outcome has caused widespread concern. direct person-to-person transmission has been documented and a segregation policy of cf patients colonized with b.cepacia from non-colonized cf patients is widely adopted. since this policy has a dramatic impact on social behaviour of cf patients it is imperative that clinical labo ...	1996	8858887
immunobiological diagnosis of tropical ocular diseases: toxocara, pythium insidiosum, pseudomonas (burkholderia) pseudomallei, mycobacterium chelonei and toxoplasma gondii.	of the sera obtained from 18 patients with ocular diseases comprising ocular larva migrans (olm), toxoplasma gondii, mycobacterium chelonei, pythium insidiosum bacteria and tumour, 3 sera were positive for toxocara antibody at the titre over 1/1600 elisa. all 3 of these sera came from males with unilateral grayish fundi. we demonstrated the value of the direct immunofluorescent assay (difa) on vitreous specimens from 7 cases of toxoplasma retinitis. one vitreous specimen under electronmicroscopi ...	1996	8880376
antimicrobial activity of superoxidized water.	we tested the antimicrobial activity of superoxidized water against methicillin-sensitive staphylococcus aureus, methicillin-resistant staphylococcus aureus, staphylococcus epidermidis, serratia marcescens, escherichia coli, pseudomonas aeruginosa and burkholderia cepacia. the number of bacteria was reduced below detection limit following incubation in superoxidized water for 10 s. the bactericidal activity of superoxidized water was similar to that of 80% ethanol, but superior to that of 0.1% c ...	1996	8880549
bacterial contamination of commercially available ethacridine lactate (acrinol) products.	bacterial contamination of commercially available ethacridine lactate (acrinol) solutions and cotton gauze soaked in ethacridine lactate solution was investigated. of 56 samples from ethacridine lactate solutions (eight products, seven manufacturers), seven samples (12.5%) of two products (two manufacturers) were contaminated with 10(1)-10(4) colony forming units (cfu)/ml of burkholderia pickettii. of 67 samples obtained from gauze soaked in ethacridine lactate solution (seven products, seven ma ...	1996	8880550
an outbreak of pseudobacteraemia caused by burkholderia pickettii: the critical role of an epidemiological link.	a protracted outbreak of pseudobacteraemia caused by burkholderia pickettii is reported. initial investigation did not reveal the source of the outbreak. subsequently, careful examination of patient records provided an insight to the probable source, which was further confirmed by a case control study. it was found that 48% of cases had either hepatobiliary diseases or haematological malignancies, whereas only 15% of the controls had similar problems. furthermore, a coagulation study was perform ...	1996	8880551
distribution and transmission of pseudomonas aeruginosa and burkholderia cepacia in a hospital ward.	genotyping and antibiotic susceptibility testing were used to analyze pseudomonas aeruginosa and burkholderia cepacia strains from sink drain from 14 pediatric patients with cystic fibrosis (cf) and from hospital personnel as part of a 4 week prospective study of strain transmission in a pediatric ward. a total of 87.5% of all washbasin drains were contaminated with p. aeruginosa [10(2) to 10(5) colony forming units (cfu)/ml sink fluid], whereas b. cepacia was found only once in a sink drain. fr ...	1996	8882212
assay for bacteria in porous media by diffusion-weighted nmr.	in this work, an nmr technique capable of detecting bacterial cells and measuring the cell density in suspension and in porous media has been developed. it is based on the pulsed-field-gradient technique and relies on the fact that extracellular water diffuses freely while intracellular water is completely restricted by the relatively impermeable cell wall of the bacterium. at high wave vectors, the signal from extracellular water is completely suppressed while the signal from intracellular wate ...	1996	8888588
a genetic analysis system of burkholderia cepacia: construction of mobilizable transposons and a cloning vector.	a genetic analysis system of burkholderia cepacia (bc) was developed which included transposon mutagenesis and complementation of mutation with the cloned genes of interest. to deliver the transposon in this multidrug-resistant microorganism, two plasmids, pkn30 and pkn31, were constructed which contained tn5 derivatives, tn5-30tp and tn5-31tp, respectively, carrying kmr and tpr genes. the plasmids have the origin of cole1 replication and the mobilization gene of rp4. tn5-31tp was mobilized to b ...	1996	8890733
cascade regulation of the toluene-3-monooxygenase operon (tbua1ubva2c) of burkholderia pickettii pko1: role of the tbua1 promoter (ptbua1) in the expression of its cognate activator, tbut.	burkholderia pickettii pko1 metabolizes toluene and benzene via a chromosomally encoded toluene-3-monooxygenase pathway. expression of the toluene-3-monooxygenase operon (tbua1ubva2c) is activated by the regulator, tbut, in the presence of toluene. we have identified the tbut coding region downstream of the toluene-3-monooxygenase structural genes by nucleotide sequence analysis and have shown that although tbut is similar to xylr and dmpr, two members of the ntrc family of transcriptional activ ...	1996	8892837
purification of hydroxyquinol 1,2-dioxygenase and maleylacetate reductase: the lower pathway of 2,4,5-trichlorophenoxyacetic acid metabolism by burkholderia cepacia ac1100.	the enzyme hydroxyquinol 1,2-dioxygenase, which catalyzes ortho cleavage of hydroxyquinol (1,2,4-trihydroxybenzene) to produce maleylacetate, was purified from escherichia coli cells containing the tfth gene from burkholderia cepacia ac1100. reduction of the double bond in maleylacetate is catalyzed by the enzyme maleylacetate reductase, which was also purified from e. coli cells, these cells containing the tfte gene from b. cepacia ac1100. the two enzymes together catalyzed the conversion of hy ...	1996	8900023
genomic complexity and plasticity of burkholderia cepacia.	burkholderia cepacia has attracted attention because of its extraordinary degradative abilities and its potential as a pathogen for plants and for humans. this bacterium was formerly considered to belong to the genus pseudomonas in the gamma-subclass of the proteobacteria, but recently has been assigned to the beta-subclass is based on rrn gene sequence analyses and other key phenotypic characteristics. the b. cepacia genome is comprised of multiple chromosomes and is rich in insertion sequences ...	1996	8900054
identification and sequencing of a novel insertion sequence, is1471, in burkholderia cepacia strain 2a.	a novel insertion sequence (is), is1471, has been identified which is inserted into is element is1071 possessed by plasmid pijb1 in burkholderia cepacia strain 2a. nucleotide sequencing has revealed that is1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp duplication of the target sequence is1471 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39406 da. searches of dna and protein sequence da ...	1996	8900064
structure of the o-specific polysaccharide from burkholderia pickettii strain nctc 11149.	a polymeric fraction (the putative o antigen) has been isolated from the lipopolysaccharide of the type strain of burkholderia pickettii. the components of the polymer and their molar proportions were: l-rhamnose (3), 2-acetamido-2-deoxy-d-glucose (1), and o-acetyl (1). by means of nmr studies and chemical degradations, the basic repeating-unit of the polymer was identified as a linear tetrasaccharide of the structure shown. the o-acetyl group is probably located at position 2 of the 3-substitut ...	1996	8901090
microbial contamination of antiseptics and disinfectants.	there have been a number of reports on microbial contamination of antiseptics and disinfectants. at present, however, the necessity of measures to prevent contamination do not seem to be fully appreciated. we investigated microbial contamination of antiseptics and disinfectants that are used in our hospital.	1996	8902114
burkholderia (pseudomonas) cepacia epidemiology in a cystic fibrosis population: a genome finger-printing study.	in patients with cystic fibrosis, infection with pseudomonas cepacia is associated with poor outcomes. however, the epidemiology of burkholderia cepacia is still unclear. the aim of this study was to investigate the epidemiology of burkholderia (pseudomonas) cepacia colonization among cystic fibrosis patients attending the verona cf centre, a large specialized unit to which patients from different parts of italy are admitted. we used a genome finger-printing system to analyse the nucleotidic str ...	1996	8827098
4-methylphthalate catabolism in burkholderia (pseudomonas) cepacia pc701: a gene encoding a phthalate-specific permease forms part of a novel gene cluster.	we have determined the entire nucleotide sequence of a 4.4 kbp fragment of pmop, a plasmid involved in 4-methylphthalate catabolism in burkholderia cepacia (formerly pseudomonas cepacia) pc701. two complete orfs were identified and termed mopa and mopb. mopb encodes a 4-methylphthalate permease which is a member of a superfamily of symport proteins found in both prokaryotes and eukaryotes. functionality was assigned to mopb by detailed analysis of the predicted amino acid sequence, resulting in ...	1996	8828207
purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from burkholderia sp. strain dnt.	4-methyl-5-nitrocatechol (mnc) is an intermediate in the degradation of 2,4-dinitrotoluene by burkholderia sp. strain dnt. in the presence of nadph and oxygen, mnc monooxygenase catalyzes the removal of the nitro group from mnc to form 2-hydroxy-5-methylquinone. the gene (dntb) encoding mnc monooxygenase has been previously cloned and characterized. in order to examine the properties of mnc monooxygenase and to compare it with other enzymes, we sequenced the gene encoding the mnc monooxygenase a ...	1996	8830701
nucleotide sequence analysis of a gene from burkholderia (pseudomonas) cepacia encoding an outer membrane lipoprotein involved in multiple antibiotic resistance.	antibiotic-resistant burkholderia (pseudomonas) cepacia is an important etiologic agent of nosocomial and cystic fibrosis infections. the primary resistance mechanism which has been reported is decreased outer membrane permeability. we previously reported the cloning and characterization of a chloramphenicol resistance determinant from an isolate of b. cepacia from a patient with cystic fibrosis that resulted in decreased drug accumulation. in the present studies we subcloned and sequenced the r ...	1996	8834871
laboratory investigation of ecological factors influencing the environmental presence of burkholderia pseudomallei.	factors like temperature, ph value, water content in soil, and ultraviolet rays that might have influence on the survival of environmental burkholderia pseudomallei strains were evaluated. data showed that the optimal temperature and ph value for b. pseudomallei were 24 c to 32 c and 5 to 8, respectively. water content in soil of less than 10% brought about the death of the bacteria within 70 days, while water content of more than 40% maintained bacteria life for 726 days. the bacteria were easi ...	1996	8839431
environmental sampling to detect burkholderia cepacia in a cystic fibrosis outpatient clinic.		1996	8839652
melioidosis: two indigenous cases in taiwan.	we report the first two indigenously acquired cases of melioidosis in taiwan, diagnosed by positive culture and biochemically identified using the id 32 gn system (biomerieux vitek inc, hazelwood, mo, usa). the first patient was a 75-year-old chinese woman who had not travelled abroad since her arrival from mainland china (san-tung province) 47 years ago. she presented with spontaneous bacterial peritonitis and hepatitis c-related liver cirrhosis with septic shock. burkholderia pseudomallei (for ...	1996	8840761
microbial pathogenesis in cystic fibrosis: mucoid pseudomonas aeruginosa and burkholderia cepacia.	respiratory infections with pseudomonas aeruginosa and burkholderia cepacia play a major role in the pathogenesis of cystic fibrosis (cf). this review summarizes the latest advances in understanding host-pathogen interactions in cf with an emphasis on the role and control of conversion to mucoidy in p. aeruginosa, a phenomenon epitomizing the adaptation of this opportunistic pathogen to the chronic chourse of infection in cf, and on the innate resistance to antibiotics of b. cepacia, person-to-p ...	1996	8840786
chronic granulomatous disease presenting as severe sepsis due to burkholderia gladioli.		1996	8842296
structural and immunological characterization of burkholderia pseudomallei o-polysaccharide-flagellin protein conjugates.	the o-polysaccharide moiety of burkholderia pseudomallei 319a lipopolysaccharide was covalently linked to flagellin protein isolated from the same strain. a glycoconjugate incorporating adipic acid dihydrazide as a spacer molecule elicited high-titer immunoglobulin g responses to both the protein and carbohydrate components of the construct. this immunoglobulin g was capable of protecting diabetic rats from challenge with a heterologous b. pseudomallei strain.	1996	8698517
chloroform mineralization by toluene-oxidizing bacteria.	seven toluene-oxidizing bacterial strains (pseudomonas mendocina kr1, burkholderia cepacia g4, pseudomonas putida f1, pseudomonas pickettii pko1, and pseudomonas sp. strains envpc5, envbf1, and env113) were tested for their ability to degrade chloroform (cf). the greatest rate of cf oxidation was achieved with strain envbf1 (1.9 nmol/min/mg of cell protein). cf also was oxidized by p. mendocina kr1 (0.48 nmol/min/mg of cell protein), strain envpc5 (0.49 nmol/min/mg of cell protein), and escheric ...	1996	8702263
an obligately endosymbiotic mycorrhizal fungus itself harbors obligately intracellular bacteria.	arbuscular-mycorrhizal fungi are obligate endosymbionts that colonize the roots of almost 80% of land plants. this paper describes the employment of a combined morphological and molecular approach to demonstrate that the cytoplasm of the arbuscular-mycorrhizal fungus gigaspora margarita harbors a further bacterial endosymbiont. intracytoplasmic bacterium-like organisms (blos) were detected ultrastructurally in its spores and germinating and symbiotic mycelia. morphological observations with a fl ...	1996	8702293
plasmid-encoded degradation of p-nitrophenol by pseudomonas cepacia.	a pseudomonas cepacia strain rkj 200 capable of utilising p-nitrophenol (pnp+) as the sole source of carbon, nitrogen, and energy was isolated by selective enrichment. the degradation of pnp by this strain proceeds through an oxidative route as indicated by the accumulation of nitrite molecules in the culture medium. initial studies indicate that the degradation of pnp occurs via hydroquinone as shown by thin layer chromatography and gas chromatography studies; hydroquinone is further degraded v ...	1996	8702398
burkholder meningitis.	we report two cases of burkholder meningitis. case 1 presented with subacute lymphocytic meningitis, low sugar profile in csf and positive culture for burkholder. case 2 presented with purulent meningitis that emerged during treatment of cryptococcal meningitis which, to our knowledge, has not been reported. thus, burkholder should be considered as one of the etiologic agents in this clinical setting.	1996	8708515
in vitro activities of quinolones, beta-lactams, tobramycin, and trimethoprim-sulfamethoxazole against nonfermentative gram-negative bacilli.	from 1991 to 1995, 8,975 nonfermentative gram-negative bacilli were isolated from patients at the ohio state university medical center: 71% pseudomonas aeruginosa, 14% stenotrophomonas maltophilia, 7.6% acinetobacter baumannii, and < 2% each of 25 other species. the mics of trovafloxacin (cp-99,219), ciprofloxacin, ofloxacin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, ceftazidime, cefoperazone, ceftriaxone, imipenem, tobramycin, and trimethoprim-sulfamethoxazole (tmp-smz) were ...	1996	8726011
melioidosis: a rare but not forgotten cause of fever of unknown origin.	melioidosis is widely prevalent in southeast asia and northern australia. although it is believed to pose no current threat to the populations of developed countries, the increased mobility of people around the world and of southeast asian refugees to western countries may change this. its long incubation period may put a relatively large number of currently asymptomatic people at risk. it should be considered in the differential diagnosis of any febrile illness in a person who has visited an en ...	1996	8731654
outcome for patients colonised with burkholderia cepacia in a birmingham adult cystic fibrosis clinic and the end of an epidemic.	there has been increasing concern since 1979 about the emergence of pseudomonas cepacia (burkholderia cepacia) in patients with cystic fibrosis in the uk and elsewhere. colonisation of the sputum has been shown to be associated with increased morbidity and mortality. evidence suggests person to person transmission and some centres have segregated those colonised with b cepacia from other patients with cystic fibrosis. the outcome of patients colonised by b cepacia has been studied, together with ...	1996	8733488
an immunohistochemical method for the diagnosis of melioidosis.	in order to assess the usefulness of immunohistochemistry in the diagnosis of melioidosis, an infection by burkholderia pseudomallei, polyclonal antibodies were applied to tissues from known cases of melioidosis and to other infected tissues. formalin-fixed, paraffin-embedded tissues were stained by a modified immunoperoxidase technique. in autopsy tissues with inflammatory lesions of melioidosis, the cytoplasm of phagocytes and intact bacilli, both intra- and extracellular, were stained very st ...	1996	8743829
the environmental risk factors associated with medical and dental equipment in the transmission of burkholderia (pseudomonas) cepacia in cystic fibrosis patients.	transmission of burkholderia (pseudomonas) cepacia by close contact with colonized patients is well documented, and minimizing social contact between cystic fibrosis (cf) patients by segregation and cohorting of b. cepacia colonized patients has achieved some success in controlling the nosocomial and community spread of this organism. however, direct and indirect environmental transmission still occurs. there is evidence for transmission of b. cepacia to cf patients via pulmonary test equipment, ...	1996	8744509
survival of burkholderia cepacia on environmental surfaces.	burkholderia (pseudomonas) cepacia is an important pathogen amongst persons with cystic fibrosis (cf), and evidence suggests that transmission of strains within cf clinics contributes to pulmonary colonization of some patients. in order to optimize preventive strategies, the survival of b. cepacia on various environmental surfaces, including cotton cloth, stainless steel, latex and polyvinylchloride (pvc) tubing, was investigated. for surface inoculation, bacteria were suspended in phosphate buf ...	1996	8744511
[structure of the o-specific polysaccharide chain from burkholderia (pseudomonas) solanacerum icmp 750, 8093, 8115, 7864, and 7945 lipopolysaccharides].	lipopolysaccharides (lps) have been isolated from the cells of phytopathogenic bacteria burkholderia (pseudomonas) solanacearum strains (representatives of i, ii and iv biovars) using mild acid hydrolysis followed by gel permeation chromatography of carbohydrate residues on sephadex g-50 to yield five o-specific polysaccharides (o-ps). the o-ps structures were established by 13c-nmr spectroscopy. it has been found that o-ps have a linear or branched structure. the former represents a tetrasaccha ...	1996	8754268
[antigenic activity of burkholderia solanacearum lipopolysaccharides].	the interaction of the antiserum against burkholderia solanacearum icmp 8110 cells with lipopolysaccharides from 19 strains of b. solanacearum differing in o-specific polysaccharide structure has been studied using elisa. no correlation was found between the o-specific polysaccharide structure and serological activity of the lipopolysaccharide. most of the lipopolysaccharides isolated from b. solanacearum strains (except for 4157, 767 and 7942) displayed cross-reactivity with the tested antiseru ...	1996	8754270
pseudomonas (burkholderia) cepacia in children with cystic fibrosis: epidemiological investigation by analysis or restriction fragment length polymorphism.	since 1987, pseudomonas cepacia has been isolated with an increasing frequency in the expectorants of children with cystic fibrosis followed at the hôpital d'enfants armand trousseau (paris, france). colonization by p. cepacia may be responsible for serious secondary infections and rapid deterioration in respiratory function in these patients. among the 130 children attending our centre, 14 (8 girls and 6 boys) aged 3 to 18, exhibited chronic colonization. 132 isolates, originating from sputum o ...	1996	8758491
2,4-dinitrotoluene dioxygenase from burkholderia sp. strain dnt: similarity to naphthalene dioxygenase.	2,4-dinitrotoluene (dnt) dioxygenase from burkholderia sp. strain dnt catalyzes the initial oxidation of dnt to form 4-methyl-5-nitrocatechol (mnc) and nitrite. the displacement of the aromatic nitro group by dioxygenases has only recently been described, and nothing is known about the evolutionary origin of the enzyme systems that catalyze these reactions. we have shown previously that the gene encoding dnt dioxygenase is localized on a degradative plasmid within a 6.8-kb nsii dna fragment (w.- ...	1996	8759857
molecular cloning and overexpression of a glutathione transferase gene from proteus mirabilis.	the structural gene of the proteus mirabilis glutathione transferase gstb1-1 (gstb) has been isolated from genomic dna. a nucleotide sequence determination of gstb predicted a translational product of 203 amino acid residues, perfectly matching the sequence of the previously purified protein [mignogna, allocati, aceto, piccolomini, di ilio, barra and martini (1993) eur. j. biochem. 211, 421-425]. the p. mirabilis gst sequence revealed 56% identity with the escherichia coli gst at dna level and 5 ...	1996	8761466
douglas-fir root-associated microorganisms with inhibitory activity towards fungal plant pathogens and human bacterial pathogens.	a microbial culture collection composed of 1820 bacterial strains, including 298 actinomycete strains, was established from the roots of douglas-fir (pseudotsuga menziesii (mirb.) franco) seedlings harvested from conifer nurseries and forest sites. two hundred and thirty-four strains inhibited the growth of fusarium, cylindrocarpon, and (or) pythium spp. in in vitro assays. a significantly greater proportion of bacterial strains from actinomycete genera exhibited antifungal properties compared w ...	1996	8764683
a phosphonate monoester hydrolase from burkholderia caryophilli pg2982 is useful as a conditional lethal gene in plants.	a bacterial phosphonate monoester hydrolase was evaluated in plants as a conditional lethal gene useful for cell ablation and negative selection. glyphosate is a potent herbicide whereas its phosphonate monoester derivative, glyceryl glyphosate, is approximately 50-fold less active. a phosphonate monoesterase gene (peha) encoding an enzyme that hydrolyzes phosphonate esters including glyceryl glyphosate to glyphosate and glycerol was cloned from the glyphosate metabolizing bacterium, burkholderi ...	1996	8771792
characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from burkholderia sp. strain rasc.	the findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-d) are typically encoded on broad-host-range plasmids. however, characterization of plasmid-cured strains of burkholderia sp. strain rasc, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-d catabolic genes were located on the chromosome of this strain. mutants of burkholderia strain rasc unable to degrade 2,4-d (2,4-d- strains) were ob ...	1996	8779585
capture of a catabolic plasmid that encodes only 2,4-dichlorophenoxyacetic acid:alpha-ketoglutaric acid dioxygenase (tfda) by genetic complementation.	the modular pathway for the metabolism of 2,4-dichlorophenoxyacetic acid (2,4-d) encoded on plasmid pjp4 of alcaligenes eutrophus jmp134 appears to be an example in which two genes, tfda and tfdb, have been recruited during the evolution of a catabolic pathway. the products of these genes act to convert 2,4-d to a chloro-substituted catechol that can be further metabolized by enzymes of a modified ortho-cleavage pathway encoded by tfdcdef. given that modified ortho-cleavage pathways are comparat ...	1996	8779586
no cross-reactivity with filamentous hemagglutinin of bordetella pertussis in sera from patients with nontypable haemophilus influenzae pneumonia.		1996	8783367
nosocomial outbreak of burkholderia pickettii infection due to a manufactured intravenous product used in three hospitals.	forty-six cases of nosocomial infection caused by burkholderia pickettii were reported between june and november 1993 in three metropolitan hospitals in madrid. a case-control study of the outbreak was conducted to identify its cause. seventy-four percent of the patients were males; the mean age +/- sd of the patients was 54 +/- 20 years. sixty-five percent of the patients presented with some gastrointestinal disorder, and 80% had a peripheral catheter; 98% were treated with intravenous fluids, ...	1996	8783718
identification of is1356, a new insertion sequence, and its association with is402 in epidemic strains of burkholderia cepacia infecting cystic fibrosis patients.	burkholderia cepacia is now recognized as an important opportunistic pathogen in cystic fibrosis (cf) and other compromised patients. epidemicity among cf patients has been attributed to at least one particularly infectious strain (strain et12), and both genetic evidence and anecdotal evidence suggest that this strain, currently endemic in ontario, and those causing an epidemic in the united kingdom, are indeed the same. our study was conducted to determine whether there was any association betw ...	1996	8784555
differentiation of thermolysins and serralysins by monoclonal antibodies.	two monoclonal antibodies (mabs) to a 36-kda extracellular metalloprotease (pscp) from burkholderia (pseudomonas) cepacia were found to react with thermolysin, pseudomonas aeruginosa elastase, alkaline protease (apr) and lasa, serratia marcescens protease (smp), aeromonas hydrophila protease (ahp), and both the lethal factor (lf) and protective antigen (pa) of bacillus anthracis on immunoblots. the mabs were capable of neutralising the proteolytic activity of thermolysin, p. aeruginosa elastase ...	1996	8810950
accuracy of four commercial systems for identification of burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis.	burkholderia cepacia has recently been recognized as an important pathogen in chronic lung disease in patients with cystic fibrosis (cf). because of the social, psychological, and medical implications of the isolation of b. cepacia from cf patients, accurate identification of this organism is essential. we compared the accuracies of four commercial systems developed for the identification of nonfermenting, gram-negative bacilli with that of conventional biochemical testing for 150 nonfermenters ...	1996	8815102
rapid antigen detection assay for identification of burkholderia (pseudomonas) pseudomallei infection.	a simple antigen detection test was developed for rapid diagnosis of melioidosis caused by burkholderia (pseudomonas) pseudomallei infection. the method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for b. pseudomallei. the results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.	1996	8815121
treatment of cystic fibrosis in adults.	more patients with cystic fibrosis are surviving into adulthood. primary care physicians need a basic understanding of adult cystic fibrosis and the evaluation of patients with acute decompensation. respiratory decompensation is usually the result of infective agents, including pseudomonas aeruginosa, stentrophomonas maltophilia and burkholderia cepacia, and requires treatment with intravenous antibiotics. if symptoms worsen, the possibility of another complication, such as pneumothorax, hemopty ...	1996	8816573