Publications

use of hydrophobic cloths as antibody adsorbents for enzyme immunoassay: detection of brucella antigens.	a cloth-elisa (c-elisa) antigen capture assay for the detection of brucella melitensis and b. abortus was developed. segments (6-mm squares) of hydrophobic polyester cloth coated with diluted serum from a b. abortus-infected cow were incubated with saline suspensions of heat-killed brucella cells, or with cultures of bovine or sheep blood or bovine tissue homogenates that had been inoculated with b. abortus or b. melitensis added to trypticase soy broth (tsb) and incubated for 2-3 days. the capt ...	1989	2505434
humoral immunity in mice mediated by monoclonal antibodies against the a and m antigens of brucella.	all smooth strains of brucella bear two lipopolysaccharide (lps) antigens in a ratio that defines the classification of strains in serovars, a (a greater than m), m (m greater than a) and a.m (a = m). anti-lps-a monoclonal antibodies (mab-a) were previously shown to convey protection to mice against b. abortus (a) strain 544, as shown by lower spleen counts than in controls at days 7 and 21 after challenge. anti-lps-m monoclonal antibodies (mab-m) were obtained and tested for m-specificity with ...	1989	2506351
comparison of protein components of different species and strains of brucella.	the proteins constituents of brucella abortus, brucella melitensis and brucella ovis were analyzed sds-page. from the comparison appears that the three species of brucella studied shows a different electrophoretic pattern specially at the level of small peptides. on the contrary when two strains of b. abortus are analyzed no differences can be noticed.	1989	2506718
evaluation of humoral immunity to brucella sp in cattle by use of an agar-gel immunodiffusion test containing a polysaccharide antigen.	results of a double agar gel immunodiffusion (ouchterlony) test that contained a polysaccharide (poly-b) antigen of brucella melitensis strain b115 were compared with those of 5 other serotests. to determine the sensitivity and specificity of the immunodiffusion, standard tube, 2-mercaptoethanol, rivanol, card, and complement fixation tests, sera obtained from 1,328 vaccinated, infected, and seronegative cattle, 56 of which had been examined bacteriologically, were used to evaluate the humoral r ...	1989	2515779
pulmonary brucellosis.	pulmonary abnormalities in brucellosis are rare. we report on nine cases (five adults and four children) with pulmonary brucellosis. all presented with fever, cough and mucopurulent sputum, and most had abnormal signs in the chest. radiography of the chest showed pneumonic patches or consolidation in five patients, pleural effusion in three, granuloma of the lung in one and a picture of interstitial pneumonitis in one. all the patients had a brucella agglutination titre of 1:320 or more, and an ...	1989	2594962
antibacterial activity of norfloxacin against 1700 relatively resistant clinical isolates.	a total of 1724 clinical isolates from patients with different infections were tested in vitro to determine their susceptibility to norfloxacin. antibacterial activity of norfloxacin was compared with other drugs that were commonly used in the hospital. of the 919 strains of enterobacteriaceae tested, all except four isolates of enterobacter, two of serratia and one of proteus were susceptible to norfloxacin. ninety-five percent of the 199 strains of pseudomonas aeruginosa were also inhibited by ...	1989	2598776
enhancement of the antigen-binding capacity of incomplete igg antibodies to brucella melitensis through fc region interactions with staphylococcal protein a.	incomplete igg anti-brucella antibodies in human sera were detected using both a conventional elisa and a modified method. in the elisa procedure serum igg was allowed to bind solid-phase b. melitensis antigen and, after washing, biotinylated staphylococcal protein a (biospa) was used as an fc-specific tracer followed by streptavidin-hrp conjugate. in the modified method, serum igg was co-incubated with a defined quantity of biospa in the presence of solid-phase antigen. biospa bound fc regions ...	1989	2600428
horizontal transmission in sheep and delayed clearance in guinea pigs and mice of a brucella melitensis rev. i mutant.	virulence assays in guinea-pigs, mice and sheep with a rev. i mutant strain (fsa) demonstrated that the latter had increased virulence in comparison with a reference strain. the mutant showed slower clearance in guinea-pigs and mice when compared with standard rev. i strains and horizontal transmission was achieved in 2 sheep.	1989	2607538
susceptibility of brucella melitensis to fluoroquinolones.	in vitro activity of four fluoroquinolones and four other antibacterial agents was tested against clinical isolates of brucella melitensis. initially all the 146 isolates studied were inhibited by 0.06-0.5 mg/l of ciprofloxacin and fleroxacin and 0.12-0.5 mg/l of pefloxacin or norfloxacin. one of these isolates developed resistance during therapy with ciprofloxacin, with a rise in mic from 0.06 mg/l to more than 5.0 mg/l. this strain also showed cross-resistance to all other quinolones. all the ...	1989	2632216
apyrexic brucella melitensis aortic valve endocarditis.	the case of a young shepherd with brucella melitensis aortic valve endocarditis is presented. his illness ran an afebrile course and was also complicated by disseminated intravascular coagulation (dic), nephritis, hepatitis and peritonitis, all of which responded well to supportive measures and a combination of tetracycline, trimethoprim-sulphamethoxazole and amikacin sulphate. the fact that even the most severe case of brucellosis can present without fever is stressed.	1989	2635455
comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of brucella ovis and brucella melitensis.	sheep infected with brucella ovis produce antibody responses to the rough lipopolysaccharide and to proteins present in hot saline (hs) extracts of b. ovis (j. i. riezu-boj, i. moriyón, j. m. blasco, c. m. marín, and r. díaz, j. clin. microbiol. 23:938-942, 1986). the distribution and antigenic relatedness of proteins in hs extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting for 41 strains of b. ovis and 26 strai ...	1989	2651307
cellular responses to "brucelline inra" during the experimental brucellosis of the mouse.	"brucelline inra" is an aqueous extract of a rough strain of brucella melitensis that is used to test cutaneous delayed hypersensitivity in man and animals. the cellular response of mice (either normal, or sensitized by a previous brucella infection) was investigated with local implanted "cell-traps" and by immunological testing in vitro. brucelline markedly reduced the number of polymorphonuclear cells in the local infiltrate of sensitized animals during the early, nonspecific phase of the resp ...	1989	2659500
laboratory survey of fluoroquinolone activity.	fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ...	1989	2672262
brucella endocarditis. report of one case and review of the literature.	we report a case of brucella endocarditis on a native aortic valve causing severe aortic regurgitation, uncontrolled heart failure and disseminated intravascular coagulopathy. the diagnosis was proven by positive serology, isolation of brucella melitensis from preoperative blood cultures and excised valve. the patient was successfully treated with valve replacement and specific antibiotic therapy.	1989	2681218
acute pancreatitis associated with brucellosis.	to the best of our knowledge, no case of acute pancreatitis associated with brucellosis has been reported. this report details the occurrence of acute pancreatitis in which brucella melitensis was cultured from blood. we discuss various diagnostic tests and the recommended therapy for brucellosis.	1989	2688402
neurobrucellosis in children.	reports on nervous system involvement in brucellosis are rare in children. we report nine children with neurobrucellosis. the clinical presentation included meningitis in six patients, one with encephalitis, one with meningoencephalitis and one with meningomyeloencephalitis. the blood from all patients showed elevation in brucella microagglutination test titers (greater than or equal to 1:640) and in brucella-specific enzyme-linked immunosorbent assay for igm (greater than or equal to 1:800), ig ...	1989	2704607
brucellosis: an unusual cause of a child's fever of unknown origin.	a 3-year-old wisconsin native with the symptoms of chronic fever, hepatosplenomegaly, and inability to walk, was found to have an elevated anti-brucella titer. blood and bone marrow cultures grew brucella melitensis, biotype 3. this infection was likely acquired during a trip to mexico several months earlier during which the child had ingested raw milk. the patient's father was also discovered to have brucellosis. both were successfully treated with antibiotic therapy. although brucellosis has b ...	1989	2728470
epidemiology and diagnosis of human brucellosis in jordan.	in recent reports, brucellosis has emerged as an important public health problem in the east mediterranean region and jordan is no exception. in 1986 and 1987, the minimal incidence rates per 100,000 persons in jordan were 33.2 and 46.2, respectively, with no sex difference. low incidence rates were confined to those below 4 and over 55 years of age. a seasonal incidence seen in 1986 was repeated in 1987 with maximal rates in the spring and summer months, exhibiting a strong connection with the ...	1989	2738993
brucellosis in a child complicated with multiple brain abscesses.	a 4-year-old boy developed symptoms consistent with brucellosis and was treated with combined streptomycin and tetracycline. he had a high brucella agglutinin titer. however, he continued to have headache and papilledema. a brain ct revealed 6 large abscesses. brucella melitensis was isolated from abscess material. the boy recovered completely after drainage therapy.	1989	2756344
monocyte locomotion in anergic chronic brucellosis patients: the in vivo effect of ascorbic acid.	in 14 patients suffering from relapsing chronic brucellosis who were anergic to brucella antigens, we have studied peripheral blood monocyte random migration and chemotaxis against non-specific and specific leukoattractants, as well as plasma and monocyte ascorbic acid levels. we found that all parameters studied, were significantly beneath normal, when compared to normal subjects. after the oral administration of ascorbic acid at a daily dose of 1gr for 15 consequetive days, random and directed ...	1989	2760414
determination by enzyme-linked immunosorbent assay of immunoglobulin g (igg), igm, and iga to brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis.	an enzyme-linked immunosorbent assay was used to compare brucella melitensis major outer membrane proteins (momp) and whole-cell heat-killed antigens (hk) in measuring antibrucella immunoglobulin g (igg), igm, and iga in sera of brucellosis patients and controls. antibodies to momp were generally similar to those against hk, and the correlation coefficients between the two antigens and igg, igm, and iga in patients varied between 0.73 and 0.94. both antigens are comparably suitable in detecting ...	1989	2768476
in vitro determination of phagocytosis and intracellular killing of brucella melitensis by goat's polymorphonuclear phagocytes.	one hundred and forty samples of goats serum taken from animals suspected of having brucellosis, have been studied by trying to assess the phagocytosis in vitro of brucella melitensis rev-1 by polymorphonuclear neutrophil phagocytes opsonized with antibrucella serum. the serum agglutination test and the coombs antiglobulin test were carried out with the serum samples. the opsonization ingestion, intracellular killing and germ killing power of the serum were also assessed. the results demonstrate ...	1989	2776406
the changing epidemiology of human brucellosis in texas, 1977-1986.	from 1977-1986, a total of 331 laboratory confirmed cases of human brucellosis were reported in texas. the annual number of cases ranged from 13 in 1977 to 84 in 1983. males accounted for 66% of the cases. cases ranged in age from one to 92 years, with 57% between the ages of 20 and 49 years. brucella melitensis infections accounted for 66% of the bacteriologically confirmed cases. infection with brucella canis was identified in four patients. annual incidence rates were higher in hispanics in e ...	1989	2787107
brucella melitensis cerebellar abscess.		1989	2794569
selection of a brucella vaccine strain of low residual virulence by chemical mutagenesis.	following incubation of the oral vaccine brucella suis strain 2 with diethyl sulphate (des), a mutant designated strain s105 was selected by screening surviving bacteria for reduced virulence for mice. strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. nevertheless, it was as effective as the parent strain in stimulating protective immunity to brucella melitensis when ...	1989	2795638
experimental treatment of brucella melitensis infection in sheep with oxytetracycline alone or combined with streptomycin.	the efficacy of oxytetracycline (otc) alone or combined with streptomycin in the treatment of 118 najdi ewes believed to have been naturally infected with brucella melitensis, was evaluated by culture of selected tissues and organs at slaughter. groups of sheep were given 250, 500 or 1,000 mg of otc intraperitoneally (i/p) daily for six weeks and in the respective groups at necropsy 52, 69 and 100% of sheep were found to be brucella-free. treatment with 250 mg otc (daily for six weeks i/p) combi ...	1989	2799947
discrimination between sheep antibodies to brucella melitensis and to brucella ovis.	when preparations containing smooth brucella abortus lipopolysaccharide (lps) were used as antigen in an elisa, strong positive reactions were obtained with sera from sheep infected with brucella melitensis or with brucella ovis. oxidation of the lps with sodium metaperiodate greatly reduced the extent of the cross-reactions with antisera to b. ovis, with little effect on the reactions with antisera to smooth b. melitensis. periodate oxidation of hot saline extract (hsx) antigen of b. ovis marke ...	1989	2800307
treatment of brucella melitensis infection in mice by use of liposome-encapsulated gentamicin.	liposomes with entrapped gentamicin were used to treat mice with infection attributable to brucella melitensis. liposomes bearing positive charge and formed by egg yolk lecithin, cholesterol, and stearylamine were effective in the elimination of b melitensis residing in liver and spleen. negatively charged liposomes, formed by egg yolk lecithin, cholesterol, and dicetyl phosphate were also effective in suppression of the infection in liver, but were less so in suppression of the infection in the ...	1989	2802318
comparative in vitro activities of ofloxacin, difloxacin, ciprofloxacin, and other selected antimicrobial agents against brucella melitensis.	the in vitro activities of three quinolones (ofloxacin, difloxacin, and ciprofloxacin) were compared with those of trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and rifampin against 47 brucella melitensis strains. ofloxacin was the most active of the test antimicrobial agents. it inhibited 90% of b. melitensis strains at a concentration of 0.02 micrograms/ml.	1989	2802568
[sacroiliitis caused by brucella melitensis].	technetium scintigraphy established sacroiliitis in a 21-year-old man who had complained of hip pain for two weeks. brucella melitensis was cultured from hip-joint fluid. he was treated with 160 mg trimethoprim and 800 mg sulphamethoxazole, three times daily for six weeks. he also had prophylactically 600 mg rifampicin daily by mouth for the last three weeks. he was symptom-free four weeks after onset of treatment. at follow-up one year later there was no sign of recurrence. brucella titres and ...	1989	2917488
acute calcular cholecystitis associated with brucella melitensis.		1989	2919595
characteristic cutaneous lesions in patients with brucellosis.	among 436 cases of brucellosis included in a 12-year prospective protocol, we identified 27 patients (6%) with cutaneous lesions (13 men and 14 women; mean age, 35.1 +/- 16.5 years). twenty-one patients had positive blood cultures for brucella melitensis. a disseminated violet-erythematous, papulonodular eruption (20 cases) and erythema nodosum-like lesions (three cases) were the most frequent eruptions observed, appearing during the initial episode of the disease or in relapse. histologic findi ...	1989	2923445
[persistence of serologic and allergic reactions in ewes after vaccination with the h.38 or rev.1 vaccines in a brucellosis (brucella melitensis) enzootic area].	in a flock of 2,000 ewes located in an endemic area of brucella melitensis infection, subjected to systematic vaccination of lambs either with killed and adjuvanted b. melitensis h.38 vaccine from 1973 to 1978, or with living b. melitensis rev.1 vaccine since 1979, reactions to allergic intrapalpebral test, complement fixation test and rose bengal plate test were recorded in small groups of animals vaccinated four, three, two or one years ago. four years after h.38 vaccination, 39 ewes out of 40 ...	1989	2930135
[bovine brucellosis caused by brucella melitensis in france].	each brucella species is known to have a definite host preference. in this respect, brucellosis in cattle is primarily due to b. abortus. as part of an investigation of the species and biovars responsible for bovine brucellosis in france, 312 strains have been examined since 1978 by the methods recommended by the subcommittee on the taxonomy of the genus brucella. of these, 264 (85%) were indeed classified as b. abortus members. however the 48 others (15%) had all the characteristics that define ...	1989	2930137
influence of doxycycline, streptomycin, cotrimoxazole and rifamycin on the functions of normal human neutrophils against brucella melitensis.		1989	16312472
brucellosis in patients with heart disease: when should endocarditis be diagnosed?	three patients, 2 with congenital valvular heart disease and 1 with a prosthetic aortic valve developed brucellosis. brucella melitensis was isolated from blood of all 3 patients. the clinical and microbiological features suggested brucella endocarditis and following successful antibiotic therapy, no surgery was required. the salient diagnostic features are discussed with emphasis on the management and prognosis of patients with brucella endocarditis.	1990	2073648
[brucellosis in a 3 month-old infant].	during the course of a bronchiolitis in a 3 month-old child, one blood culture yielded brucella melitensis. the diagnosis of brucellosis is difficult in infancy because the symptoms are nonspecific and the disease may be mild and self-limited. the child received only breast milk and dietetic preparations. so, the detection in the maternal serum of a significant titer of brucella agglutinin enabled us to consider the very rare human-to-human transmission from mother to child. because the efficacy ...	1990	2078109
the isolation of brucella melitensis biovar 3 from a testicular hygroma of a ram in a nomadic flock of sheep and goats in western sudan.	brucella melitensis biovar 3 was isolated from a testicular hygroma in a ram from a nomadic flock of sheep and goats serologically positive for brucellosis and with a history of occasional abortions. the affected testis was enlarged, extensively damaged and contained fluid. the isolation of this biovar identifies the need for further investigations into the prevalence of brucellosis and the particular biovars involved in nomadic animals.	1990	2079561
failure of prolonged treatment with ciprofloxacin in acute infections due to brucella melitensis.	a randomized prospective, pilot study was performed to compare the efficacy of oral ciprofloxacin (750 mg or 1000 mg bd) with standard oral antimicrobial therapy (rifampicin plus doxycycline) in the treatment of acute infection with brucella melitensis. all antimicrobial drugs were administered for 42 days. although all patients responded rapidly, five of the six patients receiving ciprofloxacin relapsed following cessation of therapy. there were no relapses among the patients who received doxyc ...	1990	2081722
failure of ciprofloxacin to eradicate brucellosis in experimental animals.	ciprofloxacin is a quinolone that penetrates well intracellularly. it was shown to be active against brucella melitensis in vitro. in this study, ciprofloxacin and tetracycline were used to treat b. melitensis and b. abortus infections in mice. the drugs were given for 7, 14, 21 and 28 days. tetracycline reduced spleen and liver weight and splenic bacterial counts and eliminated the infection in 70% of mice treated for 3 or 4 weeks. ciprofloxacin, on the other hand, did not affect liver or splee ...	1990	2093110
[gluteal abscesses caused by brucella melitensis. probable iatrogenic pathogenesis].		1990	2099862
experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination.	salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ...	1990	2101486
thyroiditis due to brucella melitensis--report of two cases.	two cases of thyroiditis due to brucella melitensis are reported. both occurred in foreigners working in switzerland and presented with characteristic symptoms of thyroiditis which masked those of brucellosis. cultures were diagnostic. one patient had concomitant thyroid malignancy. both showed normal thyroid function and were cured following antibiotic treatment.	1990	2114370
brucella suis s2, brucella melitensis rev. 1 and brucella abortus s19 living vaccines: residual virulence and immunity induced against three brucella species challenge strains in mice.	live attenuated brucella suis s2 vaccine was compared to living vaccines b. abortus s19 and b. melitensis rev. 1 in mice. residual virulence was estimated by ability to multiply and persist in spleen and lymph nodes. immunogenicity was estimated by spleen counts of control and vaccinated mice challenged either with the reference b. abortus 544 strain or with virulent b. melitensis h38 and b. suis 1330 strains. s2 vaccine had lower residual virulence; expressed as 50% recovery time, persistence w ...	1990	2123586
[simultaneous isolation of brucella melitensis and mycobacterium tuberculosis in pleural empyema].		1990	2129196
[a case of human brucellosis caused by accidental injection of animal vaccine].	the authors describe the case of an acute brucellosis in a young veterinarian due to accidental injection of animal vaccine. the past history of the patient and the serological follow-up of the animals contacted by the professional did not evidence other risk contacts. the case evidence the real possibility that attenuated brucella melitensis strains used in animal's vaccination can represent a source of occupational risk for this infection. in addition, it is important to notify similar cases t ...	1990	2136335
isolation, identification, and characterization in israel of brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant.	during characterization by conventional biotyping tests of numerous brucella melitensis isolates obtained in israel in the last 2 years, we identified several strains of the biovar 1 serotype that are atypically susceptible to dyes and penicillin. their coisolation from two brothers in a family that rears sheep and from the milk of one of their sheep and the prevalence of such strains in two distinct geographical zones in israel provide epidemiological support for the notion that a new variant h ...	1990	2191005
multicenter prospective study of treatment of brucella melitensis brucellosis with doxycycline for 6 weeks plus streptomycin for 2 weeks.	the effectiveness of treating human brucellosis caused by brucella melitensis with a 6-week course of doxcycline plus streptomycin for 2 of those weeks was analyzed by a multicenter prospective study of 139 patients. subjects with central nervous system involvement, endocarditis, or spondylitis were excluded from the study. all but 5 of the 139 patients completed the full treatment schedule and became afebrile in the first week of therapy. four patients suffered relapses during the follow-up per ...	1990	2193624
the interaction of brucella melitensis 16-m and caprine polymorphonuclear leukocytes.	in the present work, a study about the phagocytosis and intracellular killing of the polymorphonuclear leukocytes (pnmls) of goat in animals clinically healthy, vaccinated and inoculated experimentally with brucella melitensis has been done. during 6 weeks postvaccination and postinfection, the evolution in these animals has been studied. although animals were inoculated or vaccinated, there was influence in the phagocytosis and intracellular killing phases, even though a very low indices in thi ...	1990	2208970
the isolation and serology of brucella melitensis in a flock of goats in central rsa.	brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (boer goat) foetus in the district of cullinan near pretoria. the 18 does and 1 ram in the flock of boer goates were examined serologically by means of the complement fixation (cf) test, using brucella abortus antigen. six weeks later they were examined again, using b. abortus as well as b. melitensis biotype 1 antigens. no significant diffe ...	1990	2216349
analysis of immune response: comparison of immunoblots after isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis using cytoplasmic protein extract from brucella.	analysis of the immune response towards the facultative intracellular bacterium, brucella melitensis, was studied by immunoblotting after either isoelectric focusing (ief) or sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). a cytoplasmic extract (cpe) of brucella melitensis was used as antigen to analyse the response in 17 sera from naturally infected goats. cpe analysed by ief exhibited 25 proteins within the ph range of 4.35 to 6. immunoblotting revealed most of the staine ...	1990	2227361
concomitant neurocysticercosis and brucellosis.	a young mexican woman had headache and left arm weakness develop shortly after immigrating to the united states. a solitary cerebral cysticercus was found at surgery, but, instead of the expected finding of clear fluid, the cyst contained pus from which brucella melitensis was cultured. although the patient had no signs or symptoms suggestive of brucellosis, agglutination studies revealed igm and igg antibodies consistent with active brucellosis. clinicians should be alert to the possibility of ...	1990	2244597
osteoarticular brucellosis in children.	the findings in 40 children (24 female, 16 male) with osteoarticular complications of brucellosis are presented. raw milk was the main source of infection. most patients had acute onset of fever, arthralgia, and myalgia. arthritis was the presenting symptom in all patients, of whom two also had osteomyelitis. arthritis was monoarticular in 28 (70%) of 40 cases with predilection for large weight-bearing joints. spine and small joints were spared. brucella melitensis was detected in 23/31 (74%) ca ...	1990	2256735
the effect of the ph of various dairy products on the survival and growth of brucella melitensis.	in this study we report the effect of the ph of various dairy products on the survival and growth of brucella melitensis. the growth patterns of b. melitensis in broth media at different phs (ranging between 3 and 9) were studied for up to four weeks, to standardize the growth of the organism at each ph. these growth patterns were compared with those of the same organism growing in different dairy products [milk, soft cheese, yoghurt, and buttermilk (shaneena)] under the same growth conditions. ...	1990	2256775
[childhood brucellosis in the negev].	33 children (22 girls) with brucellosis seen between 1972-1988 were studied retrospectively. all but 1 were bedouins. the mean age at diagnosis was 9.8 years (range: 17 months-17 years). duration of illness prior to diagnosis was less than 1 week in 13 (39%), 1-4 weeks in 8 (24%) and 1-3 months in 10 (30%). in 2 cases the symptoms lasted 6 and 8 months, respectively, before diagnosis. presenting symptoms included fever (85%), articular involvement (65%), hepatomegaly (45%) and splenomegaly (33%) ...	1990	2283118
brucella meningitis.	a 36-year-old hispanic man came into the emergency department with nonspecific symptoms (headache, myalgias, low-grade temperature, and low white blood cell count) and was diagnosed with brucella meningitis. the patient said he had consumed unpasteurized goat's milk and cheese in mexico, and had been treated 3 months previously for a febrile illness diagnosed as malta fever (brucellosis). cultures of both the blood and cerebrospinal fluid yielded brucella melitensis. blood agglutinin results for ...	1990	2293833
diagnosis and treatment of 106 cases of human brucellosis.	during the year 1987, 106 cases of human brucellosis were studied prospectively at the jordan university hospital. the disease was more often diagnosed among adults (73.6%) than children (26.4%). serious clinical complications were observed in 5.7% patients. an initial brucella antibody titre greater than or equal to 160 proved to be reliable in confirming suspected cases of acute and subacute brucellosis. culture of blood was found to be more sensitive (44.4%) and significant (p less than 0.02) ...	1990	2299182
isolation of brucella melitensis biovar 3 from a chamois (rupicapra rupicapra) in the southern french alps.	systemic brucellosis caused by brucella melitensis biovar 3 was identified in a chamois (rupicapra rupicapra) near the parc national des ecrins in the southern french alps (france). clinical signs included orchiepididymitis, polyarthritis, blindness and various neurological signs; necropsy findings included numerous calcified foci in testis, epididymis, kidney, subcutaneous connective tissue and brain. brucella sp. were identified in brain by indirect immunofluorescence and b. melitensis biovar ...	1990	2304191
childhood brucellosis: a study of 102 cases.	one hundred two children, 45 days to 14 years of age, with proven brucellosis were studied to illustrate the epidemiologic, clinical and laboratory findings and to assess the outcome of antimicrobial therapy. the main source of infection was the consumption of raw milk in 80% of the patients. the predominant presenting symptoms and signs were fever, arthralgia, malaise, weight loss, arthritis, hepatosplenomegaly and lymphadenopathy. brucella melitensis was isolated from 75% of 87 patients. diver ...	1990	2314956
development of ciprofloxacin resistance in brucella melitensis.		1990	2329110
ocular brucellosis.	though brucellosis has been eradicated from many countries, it remains a health problem in many developing countries. we report hereon a 34-year-old woman who presented with recurrent attacks of uveitis unresponsive to treatment with steroids. she was found to have systemic brucellosis, and brucella melitensis was isolated from a paravertebral abscess. the condition responded to systemic antibiotics, and she completely recovered from her uveitis.	1990	2337555
isolation of brucella melitensis from human sperm.		1990	2351148
chronic neurobrucellosis due to brucella melitensis.	a 20-year-old male turkish immigrant to norway suffering from severe chronic neurobrucellosis with spastic paraplegia and deafness is presented. the diagnosis was established by isolation of brucella melitensis from cerebrospinal fluid (csf) culture. brucella antibody agglutination titers were high in serum and csf. in spite of intensive, prolonged treatment with a combination of trimethoprim-sulfamethoxazole (tpm-smz), rifampicin and doxycycline, the course of the illness was characterized by r ...	1990	2356445
serology, clinical manifestations and treatment of brucellosis in different age groups.	we prospectively studied 339 patients diagnosed of brucellosis over a six year period in order to evaluate the clinical and serological characteristics of brucellosis in the elderly. 319 patients (94.1%) were under 65 years of age (group a), and 20 patients (5.9%) were older than 65 (group b). no patient in group b developed splenomegaly as opposed to 69 (21.6%) in group a (p less than 0.05). the percentage of positive blood cultures (brucella melitensis isolated in all cases) was 72.1% in group ...	1990	2365466
antibacterial activity of lomefloxacin against brucella melitensis.	the in vitro activity of lomefloxacin was tested against 114 clinical isolates of brucella melitensis. comparison was made with ciprofloxacin, tetracycline, gentamicin, rifampin, and trimethoprim-sulfamethoxazole. lomefloxacin inhibited 113 (99.1%) of the 114 strains tested at less than or equal to 0.5 microgram/ml. it was comparable to ciprofloxacin, tetracycline and gentamicin in antimicrobial potency. one strain that was previously susceptible to ciprofloxacin and had become resistant after t ...	1990	2383977
recurrent enterocolitis-like symptoms as the possible presenting manifestations of neonatal brucella melitensis infection.	a premature infant had three episodes of enterocolitis-like symptoms during the first three months of life. brucella melitensis was isolated in a prolonged blood culture on day 85. the clinical manifestations disappeared under treatment and did not recur. a maternofetal transmission is suggested in this case. the importance of prolonged blood cultures when brucellosis is epidemiologically suspected is emphasized.	1990	2386068
successful treatment of brucella melitensis endocarditis with pefloxacin.		1990	2387299
brucella melitensis endogenous endophthalmitis.	a 17-year-old female patient was examined and found to have brucella-sp.-induced endophthalmitis in her left eye. the specific organism was brucella melitensis, biotype 3, a known virulent organism. serum agglutination titres from two independent laboratories were positive, yet of different levels: 1:320 and 1:1,280. although brucellosis is thought to be eradicated in many countries, it prevails in agrarian environments, where it can be transmitted to man. this report is the first evidence to co ...	1990	2392274
antigenic relationships of the lipopolysaccharides of escherichia hermannii strains with those of escherichia coli o157:h7, brucella melitensis, and brucella abortus.	clinical isolates of escherichia hermannii which showed serological cross-reaction with polyclonal antisera to the o-polysaccharide portion of the lipopolysaccharide of e. coli o157 strains and with antisera to the o antigens of brucella abortus and b. melitensis were found by chemical and nuclear magnetic resonance analyses to have lipopolysaccharide o chains composed of linear polymers containing 1,2- and 1,3-linked 4-acetamido-4,6-dideoxy-alpha-d-mannopyranosyl (alpha-d-rhap4nac) residues. tw ...	1990	1691146
[cloning of brucella genes in escherichia coli k12 cells and analysis of products of the cloned genes].	the libraries of brucella melitensis 565 and brucella abortus 99 in escherichia coli cells have been constructed. some clones of escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. two strains producing antigens have been characterized, one being from brucella melitensis 565 and another from brucella abortus 99 clone libraries . both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting aft ...	1990	1701518
probable breast-milk borne brucellosis in a young infant.	a young infant with acute brucellosis is reported. he presented with a septicaemia-like picture. diagnosis was based on a fourfold rise of brucella agglutination titres and a positive blood culture. he had been exclusively breast-fed when his mother developed brucellosis 4 weeks after delivery. it is strongly suspected that the transmission of brucella melitensis to this infant was through the maternal breast-milk.	1990	1703749
[the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9].	antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ...	1990	1704479
inhibition of the degranulation and myeloperoxidase activity of human polymorphonuclear neutrophils by brucella melitensis.	the degranulation and myeloperoxidase-h2o2-halide activities of human polymorphonuclear leukocytes from healthy donors were tested after co-incubation with either brucella melitensis 16m, staphylococcus aureus or staphylococcus aureus in presence of lipopolysaccharide, protein fraction, native hapten and soluble fractions released at 65 degrees c from smooth strain of brucella melitensis 16m. the degranulation and myeloperoxidase activities of polymorphonuclear leukocytes were significantly high ...	1991	1662050
microbiological test strip (api20ne) identifies brucella melitensis as moraxella phenylpyruvica.		1991	1669878
brucellosis surveillance.		1991	1669892
person-to-person transmission of brucella melitensis.	human brucellosis is primarily an occupational hazard in the usa; in the middle east and africa ingestion of contaminated dairy products is an important route of infection. whether human beings can become infected via person-to-person spread is uncertain. during an investigation of a commonsource, laboratory-associated outbreak due to brucella melitensis, biotype 3, the wife of a microbiologist with serologically proven brucellosis became infected. her blood isolate was indistinguishable from th ...	1991	1670649
transmission of brucella melitensis.		1991	1672931
[brucellar pericarditis: 2 different forms of presentation for an unusual etiology].	two cases of brucella melitensis pericarditis are reported. pericardial involvement was the first and almost only manifestation of brucellosis in the first patient while in the second, a significant pericardial effusion was discovered on a routine echocardiogram performed in a patient with clinically florid brucellosis. some etiopathogenic aspects of this uncommon etiology are discussed.	1991	1759029
brucellosis: an unusual diagnosis for a seronegative patient with abscesses, osteomyelitis, and ulcerative colitis.	a 16-year-old girl developed multiple subcutaneous abscesses, osteomyelitis, and severe colitis. on the patient's second admission, a single blood culture--and, subsequently, a specimen of pus--yielded brucella melitensis biovar 1. a second set of serologic tests, including the rose bengal test, the standard tube agglutination test, the cf test, and coombs' test, were all negative for brucella on the patient's second admission and 1 month later. however, a lymphocyte proliferation assay with ext ...	1991	1775834
brucella melitensis rev. 1 living attenuated vaccine: stability of markers, residual virulence and immunogenicity in mice.	five commercial brucella melitensis rev. 1 vaccines from different sources were compared to the original elberg rev. 1 strain, in vitro for classic markers and in vivo in mice, for residual virulence and immunogenicity. because colonies of several morphology types (smooth, non-smooth) were isolated from the vaccines, representative substrains were purified to study their in vitro and in vivo activities either at once (16 strains), or after storage by subculture (12 strains) and by lyophilization ...	1991	1797046
characterization of smooth brucella lipopolysaccharides and polysaccharides by monoclonal antibodies.	two mouse monoclonal antibodies (mab) generated to the m antigen of brucella melitensis 16m were analysed. binding profiles of both monoclonals were established by a competitive enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides, o polysaccharides and native hapten polysaccharides from b. melitensis 16m, b. abortus 544 and yersinia enterocolitica o:9. using this assay, significant differences in the reactivity of both antibodies were found with a and m antigens from b ...	1991	1805311
[effect of the extraction of lps (r) on the demonstration of the serological response in acute brucellosis by immunoblotting].	in order to establish the possible influence of brucella melitensis rough strains protein extracts contamination using lps (r) in the immunoblotting evaluation of serologic response, we have studied sera samples from 41 patients with acute brucellosis before treatment was started and also from 62 healthy individuals (control group). a whole-cell b. melitensis 115 strain extract with an without lps (r) was used. extraction was carried using ether-chloroform of oil-phenol. overall, more than 50% o ...	1991	1805947
induction of antibody and protective responses in mice by brucella o-polysaccharide-bsa conjugate.	a brucella conjugate vaccine was synthesized by covalently coupling the o-polysaccharide (ps) obtained from brucella melitensis 16m, to bovine serum albumin (bsa). the conjugate (ps-bsa) was non-toxic for mice. when injected at two doses (10 or 1 micrograms) in mice, it induced the production of antibodies and the boost was shown to be efficient if administered after 10 weeks. immunization of mice with 10 micrograms of conjugate conferred significant protection against virulent challenge with b. ...	1991	1811374
brucellosis as a cause of backache.	prospective screening of 173 patients with complaints of backache and a history of ingestion of raw milk yielded 21 patients suffering from brucella spondylitis. the overall incidence of brucellosis was 28 in 10,647 patients; 14 out of 21 (67%) presented with acute signs and symptoms of brucella spondylitis and six patients required hospitalization. the majority of the patients had a positive titre of brucella melitensis and abortus. in 42% of the patients radiographs of the thoraco-lumbar spine ...	1991	1836289
[phenol-insoluble vaccine against human brucellosis: evaluation of the immunogenic power in guinea pigs].	a phenol insoluble extract vaccine proposed to immunize men against brucellosis was tested for its ability in protecting guinea pigs against challenge with virulent brucella abortus strain 2308. living attenuated brucella abortus strain 19 and b. melitensis rev. 1 were included in the experiment for comparison. ninety three animals were vaccinated in each group and subdivided in subgroups of 31 for challenge with 10(4), 10(3) and 10(2) colony forming units of virulent b. abortus 2308. a global a ...	1991	1843392
[serological survey of small ruminants in 4 districts of niger].	in 1990, a serosurvey on 1,474 small ruminants was conducted in four districts of niger (maradi, zinder, diffa and dosso). the epidemiology and seroprevalence of eight diseases were studied: brucella melitensis and b. ovis brucellosis, chlamydiosis, coxiellosis, contagious caprine pleuropneumonia, type a pasteurellosis, peste des petits ruminants, rift valley fever, crimée-congo haemorragic fever. the main health problem for the development of small ruminants farming, seems to be both pasteurell ...	1991	1843821
[fever, swelling of testis and epididymis, poly-lymphadenopathy, splenomegaly].	an 18-year-old turkish farmer was admitted to the hospital because of fever, painful scrotal swelling, generalized lymph-node enlargement and splenomegaly. after an incubation period of six days brucella melitensis was isolated in blood culture. the diagnosis of acute brucellosis with lymphadenopathy, splenomegaly and epididymitis was made. he was treated with doxycycline and rifampicin for six weeks and recovered completely. three months after therapy he felt well. a relapse did not occur.	1991	1871475
in vitro activity of lomefloxacin, a difluorinated quinolone, compared with other antimicrobials.	a total of 3,144 clinical isolates from 3,011 consecutive patients were tested against lomefloxacin by the agar dilution method. they consisted of 1,380 isolates of enterobacteriaceae, 527 pseudomonads, 47 haemophilus influenzae, 53 acinetobacter, 42 brucella melitensis, 903 staphylococci and 192 strains of enterococci. in vitro activity of lomefloxacin was compared with ciprofloxacin, norfloxacin, beta-lactams and aminoglycosides. over 98% of enterobacteriaceae were susceptible to lomefloxacin ...	1991	1889304
infections in british clinical laboratories, 1988-1989.	during 1988-89 this continuing survey showed 18 infections in the staff of laboratories reporting from 166 centres, representing 21,756 person-years of exposure. shigella and other bowel infections (one caused by s typhi) predominated, affecting 11 microbiology medical laboratory scientific officers. three shigella infections originated from quality control samples. pulmonary tuberculosis affected four workers, including two mortuary technicians, but without detected occupational exposure to myc ...	1991	1890201
[sensitivity of brucella melitensis to various antibiotics, drug preparations and their combinations].	in vitro activity of 13 antibiotics and drug preparations as well as some of their combinations was studied with respect to brucella melitensis. it was shown that the strains of b. melitensis were sensitive to sisomicin, amikacin, gentamicin, cefotaxime, rifaprim +, nitroxoline and trimethoprim. variations in the doxycycline sensitivity depending on the strain were observed. the strains of b. melitensis were resistant to cephalexin, phosphomycin, chinoxydine, dioxydine and oxolinic acid. combina ...	1991	1892428
[a case from practice (227). bang disease (brucella melitensis) with-- spondylitis c7/th1].		1991	1947557
in vitro susceptibility of brucella melitensis to antibiotics.	the in vitro susceptibilities of 86 recent clinical isolates of brucella melitensis to minocycline, streptomycin, co-trimoxazole, rifampin, and six fluoroquinolones were determined. minocycline exhibited the lowest mic and was followed by rifampin and streptomycin. among the quinolones, win 57273 and ciprofloxacin were the most active agents. no antibiotic combination of these agents exhibited synergy against 15 selected isolates. in killing rate experiments, streptomycin exhibited the most rapi ...	1991	1952869
a rare cause of colitis--brucella melitensis. report of a case.	documentation of gastrointestinal lesions in brucella infections is sparse. a case of brucella melitensis type 3 infection accompanied by erosive lesions of the colon, observed by endoscopy and histopathologic examination, is reported. such gastrointestinal lesions have not been described since 1934. before 1934 only postmortem observations are recorded.	1991	1993419
physical map of the brucella melitensis 16 m chromosome.	we present the first restriction map of the brucella melitensis 16 m chromosome obtained by southern blot hybridization of spei, xhoi, and xbai fragments separated by pulsed-field gel electrophoresis. all restriction fragments (a total of 113) were mapped into an open circle. the main difficulty in mapping involved the exceedingly high number of restriction fragments, as was expected considering the 59% g + c content of the brucella genome. several cloned genes were placed on this map, especiall ...	1991	2007548
outbreak of brucella melitensis among microbiology laboratory workers in a community hospital.	from may to september 1988, eight employees of a microbiology laboratory developed acute brucellosis (attack rate, 31%). seven of the eight affected employees had clinical illness ranging from a nonspecific, flulike illness to severe hepatitis. blood cultures obtained from five of the affected employees (63%) were positive for brucella melitensis, biotype 3. comparison of cases and controls showed that there were no risk factors besides employment in the laboratory. based on work locations, assi ...	1991	2007637
antibacterial activity of fce 22101 against relatively resistant blood culture isolates.	in vitro antibacterial activity of the new penem fce 22101 was tested against blood culture isolates from 1,374 consecutive patients in a major tertiary care referral center in saudi arabia. many of the isolates were significantly more resistant to commonly used beta lactams. of the 809 members of enterobacteriaceae tested all but 14 isolates of enterobacter were susceptible to fce 22101 with an mic range of 0.12-8.0 micrograms/ml. it inhibited all the 183 isolates of staphylococcus aureus, both ...	1991	2013238
bilateral mammary abscess and uveitis caused by brucella melitensis--report of a case.	soft tissue locations of brucella are a rare finding. we report the isolation of brucella melitensis from a suspected breast tumour of a woman who also showed signs of uveitis. three weeks after surgical drainage and despite antimicrobial therapy a new abscess developed on the other breast whose culture also yielded brucella. as observed, unusual localization of brucellosis without previous penetrating injury may be the only manifestation of chronic infection.	1991	2013508
[brucella endocarditis on starr aortic valve prosthesis].	the authors report the case of a 22 year old patient with brucella endocarditis on a starr-edwards aortic valve prosthesis implanted 5 years previously. six blood cultures were positive for brucella melitensis biovar i. antibiotic therapy associating rifampicin and tetracycline and then rifampicin and ofloxacine did not prevent abscess formation and valve dehiscence. the poor haemodynamic status and persistent infection led to replacement of the valve prosthesis. culture of the infected prosthes ...	1991	2021291
comparison of serological tests for brucella melitensis infection in sheep.	a comparative study of the standard tube agglutination test (sat), rose bengal plate agglutination test and counter immuno-electrophoresis (ciep) was made on 647 sera from naturally aborting ewes, orchitic, in-contact and apparently healthy sheep with no history of vaccination against brucellosis. no individual test could detect all the 13 known positive reactors (the foetuses of which yielded brucella melitensis) but by combination of two tests all 13 were positive. the sat detected more reacto ...	1991	2038764
identification of dye-sensitive strains of brucella melitensis.	strains of brucella melitensis biovars 1, 2, and 3 isolated in india, italy, kuwait, saudi arabia, the federal republic of germany, and zimbabwe were inhibited by thionin (20 micrograms/ml) but not by basic fuchsin (20 micrograms/ml) or safranin o (200 micrograms/ml). in other respects the strains were typical of their species and biovars. the existence of dye-sensitive strains of b. melitensis suggests that the present system of classification requires modification.	1991	2056043