Publications

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[association between bacteroides forsythus in the infected root canals and clinical symptoms of chronic apical periodontitis].to investigate the distribution of bacteroides forsythus in root canals with chronic apical periodontitis and to determine its associations with clinical symptoms.200515702778
impact of sample storage on detection of periodontal bacteria.information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration.200515720575
serum antibodies to oral anaerobic bacteria in patients with rheumatoid arthritis.this study was conducted to determine the component that causes the disease in rheumatoid arthritis (ra), which shows great resemblance to periodontitis in a pathologic context.200516369381
[colonization relationship between porphyromonas gingivalis and bacteroides forsythus in the infected root canals with chronic apical periodontitis].to detect the distribution of p. gingivalis and b. forsythus in the infected root canals from chinese chronic apical periodontitis, and investigate the colonization relationship between them in the root canals.200516288338
the effect of haart on salivary microbiota in the women's interagency hiv study (wihs).study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study.200516301151
the distribution of tannerella forsythia in an adolescent and adult population.the fact that tannerella forsythia, an important periopathogen, is difficult to cultivate from mixed infections has impeded precise estimates of its distribution within a given population. in order to discern t. forsythia alone from the mixed infection of plaque, the use of sensitive 16s ribosomal rna based polymerase chain reaction (pcr) detection is necessary.200516302927
streptococci dominate the diverse flora within buccal cells.previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ...200516304448
cytomegalovirus-associated periodontitis and guillain-barré syndrome.guillain-barré syndrome (gbs), an autoimmune disorder of the peripheral nervous system, is characterized by rapidly ascending neural paralysis, hyporeflexia, and areflexia. the polyneuropathy of the gbs affects one to four humans per 100,000 of the population annually throughout the world (adults and children). the pathogenesis of gbs remains unclear. however, there are increasing indications that the disease is triggered by a preceding well-established febrile infection by cytomegalovirus (cmv) ...200516332244
involvement of porphyromonas gingivalis fima genotype in treatment outcome following non-surgical periodontal therapy.porphyromonas gingivalis is considered a critical pathogen in periodontal diseases. it is classified into six genotypes based on diversity of the fima gene encoding fimbrillin. the present study evaluated the involvement of the fima genotype in treatment outcome following non-surgical periodontal therapy.200516253087
distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers.we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ...200616269128
isolation and identification of a cytopathic activity in tannerella forsythia.interactions between pathogens and host induce human disorders including periodontitis, disintegration of the tooth supporting tissues. tannerella forsythia has been linked to the periodontitis and several cytopathic reagents have been found in the bacterium; however, its contribution to the disease remains unclear. biochemical approach to explore the cytopathic effect revealed two distinct activities in t. forsythia (atcc 43037) extract; one detaches adherent cells from substratum and another a ...200617054916
carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification.the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola.200617064390
immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control.to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions.200616367855
subgingival microbiota of periodontally untreated mexican subjects with generalized aggressive periodontitis.specific microbial profiles that may distinguish between generalized aggressive-periodontitis (gagp) and generalized chronic-periodontitis (gcp) have, to date, not been described. the purpose of the present study was to describe the subgingival microbial composition of mexican subjects with gagp and compare it with that of individuals with gcp and periodontal health (ph).200617092239
bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis.aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ...200617098542
oral bacteria in plaque and invading buccal cells of young orthodontic patients.in this longitudinal study, patients with fixed orthodontic appliances served as models to determine whether actinobacillus actinomycetemcomitans, tannerella forsythia, and total streptococci increased after treatment, and whether treatment affected bacterial invasion into the adjacent buccal epithelial cells (bec).200617169727
prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations.evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ...200617174667
relationship of periodontal bacteria and porphyromonas gingivalis fima variations with phenytoin-induced gingival overgrowth.we investigated the relationship between phenytoin-induced gingival overgrowth (go) and the harboring of periodontal bacteria.200616390469
[microbiological study on the prognosis of immediate implant and periodontal disease].the aim of the present study was to investigate the prevalence of periodontal marker organisms and specific interleukin-1 (il-1) gene polymorphisms (which show a close association with periodontitis) and their effect on the success of immediate implant placement postextraction in the patient with periodontal disease.200616395549
the transmission of anaerobic periodontopathic organisms.the oral microbial flora is unique, and available evidence indicates that it is passed vertically from parents to children. in this investigation, we used a chairside assay for the n-benzoyl-dl-arginine-2-naphthylamide (bana)-sensitive enzyme found in porphyromonas gingivalis, treponema denticola, and tannerella forsythensis, to determine the prevalence of these bana-positive species in young children and their caregivers. we predicted that if the bana enzyme was found in plaque samples of child ...200616434739
supportive periodontal therapy using mechanical instrumentation or 2% minocycline gel: a 12 month randomized, controlled, single masked pilot study.to compare the short-term performance of subgingival local delivery of 2% minocycline gel and conventional subgingival debridement in supportive periodontal therapy (spt) patients.200616441740
comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods.studies have shown similarities in the microflora between titanium implants or tooth sites when samples are taken by gingival crevicular fluid (gcf) sampling methods. the purpose of the present study was to study the microflora from curette and gcf samples using the checkerboard dna-dna hybridization method to assess the microflora of patients who had at least one oral osseo-integrated implant and who were otherwise dentate. plaque samples were taken from tooth/implant surfaces and from sulcular ...200616441779
early colonization of non-submerged dental implants in patients with a history of advanced aggressive periodontitis.the aim of the study was to evaluate the early colonization of non-submerged implants over a 6-month period in partially edentulous patients treated for advanced aggressive periodontal disease. in 22 patients treated for advanced aggressive periodontitis and in a supportive maintenance program for a period between 12 and 240 months at implant surgery, a total of 68 non-submerged dental implants were installed. patients had a plaque score below 20%, and less than 20% of the pockets around the tee ...200616441780
periodontitis is associated with preeclampsia in pregnant women.recent investigations have demonstrated a positive association between periodontitis and pregnancy complications. the purpose of this study was to determine the effect of periodontitis and the subgingival microbial composition on preeclampsia.200616460242
characterization of patients presenting for treatment to a university refractory periodontal diseases unit: three case reports.of the many forms of periodontal disease, refractory periodontal diseases are the least characterized. they are defined as the continued degeneration of the periodontium despite adequate management. this has led to the suggestion that there may be a systemic component that is a contributing factor to the development of this condition. the objectives of this report were to follow the progression of clinical changes associated with periodontal disease over a number of years in this unique populati ...200616460260
inhibition of periodontopathogen-derived proteolytic enzymes by a high-molecular-weight fraction isolated from cranberry.porphyromonas gingivalis, tannerella forsythia and treponema denticola are three major aetiological agents of chronic periodontitis. the strong proteolytic activities of these bacteria are critical to their survival since their energy source is obtained from peptides and amino acids derived from proteins. in addition, proteases are important factors contributing to periodontal tissue destruction by a variety of mechanisms, including direct tissue degradation and modulation of host inflammatory r ...200616473919
identification and characterization of the genes encoding a unique surface (s-) layer of tannerella forsythia.a newly emerged periodontopathic pathogen tannerella forsythia (formerly bacteroides forsythus), a gram-negative, filament-shaped, strict anaerobic, non-pigmented oral bacterium, possesses a surface (s-) layer. in our previous studies, the s-layer has been isolated, and shown to mediate hemagglutination, adhesion/invasion of epithelial cell, and murine subcutaneous abscess formation. in the present study, biochemical and molecular genetic characterization of the s-layer are reported. amino acid ...200616488557
is pre-term labour associated with periodontitis in a danish maternity ward?to reveal differences in periodontal status and presence of subgingival bacteria in a scandinavian population of women with pre-term birth compared with women who delivered at term.200616489943
the efficacy of povidone-iodine products against periodontopathic bacteria.a total of 8 strains of 6 bacterial species, porphyromonas gingivalis atcc33277 and tdc286, actinobacillus actinomycetemcomitans atcc29523 and jp2, fusobacterium nucleatum no. 2, tannerella forsythensis atcc43937, prevotella intermedia atcc25611 and streptococcus anginosus atcc33397, were treated with povidone-iodine (pvp-i) gargle (pvp-i: 0.47 and 0.23% w/v) or chlorhexidine gluconate (chg) gargle (chg: 0.002% w/v) for 15, 30 or 60 s, after which they were inoculated into various media, culture ...200616490986
anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens.periodontitis is a chronic inflammatory disease affecting oral tissues. the continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. macrophages play a critical role in this host inflammatory response to periodontopathogens. the aim of this study was to investigate the effect of non-dialyzable material prepared from cranberry juice concentrate on the pro-inflammatory cytokine response of ...200616498070
description of the subgingival microbiota of periodontally untreated mexican subjects: chronic periodontitis and periodontal health.recent studies have suggested that changes in the prevalence and/or proportion of distinct microorganisms characterize the subgingival microbial profiles of populations around the world. at present, no information is available on the subgingival microbiota of mexican subjects. the purpose of the present study was to determine the microbial composition of subgingival plaque in mexican subjects with untreated chronic periodontitis.200616512761
detection of bacterial dna in atheromatous plaques by quantitative pcr.this is the first study to analyze atheromatous plaques for the presence of bacterial dna from ten species, including periodontal species and chlamydia pneumoniae. we examined 129 samples of dna extracted from atheromas from 29 individuals for the presence of bacterial 16s rdna sequences from ten different species: porphyromonas gingivalis, actinobacillus actinomycetemcomitans (a.a.), tannerella forsythensis, eikenella corrodens, prevotella intermedia, staphylococcus aureus, staphylococcus epide ...200616513386
periodontal infections and coronary heart disease: role of periodontal bacteria and importance of total pathogen burden in the coronary event and periodontal disease (corodont) study.chronic inflammation from any source is associated with increased cardiovascular risk. periodontitis is a possible trigger of chronic inflammation. we investigated the possible association between periodontitis and coronary heart disease (chd), focusing on microbiological aspects.200616534043
clinical and microbiological effects of different antimicrobials on generalized aggressive periodontitis.to evaluate and compare the effects of adjunctive metronidazole plus amoxicillin, doxycycline and metronidazole on clinical and microbiological parameters in patients with generalized aggressive periodontitis.200616553634
subgingival and tongue microbiota during early periodontitis.periodontal infections have a microbial etiology. association of species with early disease would be useful in determining which microbes initiate periodontitis. we hypothesized that the microbiota of subgingival and tongue samples would differ between early periodontitis and health. a cross-sectional evaluation of 141 healthy and early periodontitis adults was performed with the use of oligonucleotide probes and pcr. most species differed in associations with sample sites; most subgingival spec ...200616567551
relationship of serum advanced glycation end products with deterioration of periodontitis in type 2 diabetes patients.a close relationship between diabetes and chronic periodontitis has been demonstrated. we previously found that porphyromonas gingivalis with the type ii fima gene is an infectious factor closely associated with the deterioration seen in diabetic periodontitis patients. in the present study, we examined whether other biomarkers are related to the development and deterioration of periodontitis often seen in type 2 diabetic individuals.200616579698
local oxygen therapy for treating acute necrotizing periodontal disease in smokers.the main aim of treatment for acute necrotizing periodontal disease is fast and effective reduction of anaerobic destructive microorganisms to avoid periodontal damage. the effect of adjunctive local oxygen therapy in the treatment of necrotizing periodontal disease was examined in this study.200616579700
subgingival distribution of campylobacter rectus and tannerella forsythensis in healthy children with primary dentition.it is important to know how many subgingival plaque samples should be assayed from a child to ascertain infection with a periodontal pathogen. plaque samples from several sites may fail to detect some important bacteria if only a limited number of gingival sites are sampled. the purpose of this study was to evaluate the detection of periodontal pathogens in a large number of subgingival sites in the same children in order to determine the number of samples necessary.200615990083
prth in tannerella forsythensis is not associated with periodontitis.it has been suggested that prth in tannerella forsythensis encodes for a cystein proteinase that is associated with its pathogenic potential and can discriminate between periodontal health and disease. the aim of this investigation was to further establish this potentially important observation.200616584338
langerhans' cell histiocytosis in a 5-year-old girl: evidence of periodontal pathogens.langerhans' cell histiocytosis (lch) is a rare disorder characterized by langerhans' cell proliferation in various organs or tissues. when periodontal tissue is involved, clinical manifestations can vary from gingival recession and pocket formation to severe alveolar bone loss. this case report describes periodontal pathogens found in the pockets of involved primary teeth.200616584357
[quantitative analysis of periodontal pathogens in aggressive periodontitis patients in a japanese population].the aim of the present study was to clarify the relationship between the relative/absolute numbers of periodontal bacteria and different types of periodontitis. fifteen patients with localized aggressive periodontitis (lagp), 25 patients with generalized aggressive periodontitis (gagp) and 28 patients with chronic periodontitis (cp) were included in this study. saliva and subgingival plaque samples were collected from all subjects for microbiological analysis. the prevalence and proportions of a ...200616629473
supragingival plaque may modify the effects of subgingival bacteria on attachment loss.bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss.200616671872
a model of spontaneous periodontitis in the miniature goat.the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis.200616671878
prevalence of periodontopathic bacteria on the tongue dorsum of elderly people.the aim of this study was to analyse the prevalence of oral bacteria on the dorsum of the tongue. in addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed.200616677187
the additional value of real-time pcr in the quantitative detection of periodontal pathogens.for the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. the technique of real-time polymerase chain reaction(rt-pcr) provides a new tool to detect oral pathogens both in oral and non-oral human infections. the aim of this study was to compare the rt-pcr and anaerobic culture for detection and quanti ...200616677332
inflammatory responses of a macrophage/epithelial cell co-culture model to mono and mixed infections with porphyromonas gingivalis, treponema denticola, and tannerella forsythia.accumulated evidence points to porphyromonas gingivalis, treponema denticola, and tannerella forsythia as three major etiologic agents of chronic periodontitis. epithelial cells and macrophages play a major role in the host response to periodontopathogens, and the secretion of inflammatory mediators and matrix metalloproteinases (mmps) by these host cells is believed to contribute to periodontal tissue destruction. the aim of this study was to investigate the inflammatory response of a macrophag ...200616153871
in vitro antimicrobial and resistance-modifying activities of aqueous crude khat extracts against oral microorganisms.to assess antimicrobial activities of aqueous crude khat (catha edulis) extracts against a panel of oral microorganisms and to test their ability to modify bacterial resistance to tetracycline and penicillin in vitro.200616248981
influence of combinations of bacteria on the levels of prostaglandin e2, interleukin-1beta, and granulocyte elastase in gingival crevicular fluid and on the severity of periodontal disease.the aim of this study was to investigate the simultaneous presence of periodontal microbiota on inflammatory markers in gingival crevicular fluid from individuals with periodontal diseases.200616734578
bacterial profile and burden of periodontal infection in subjects with a diagnosis of acute coronary syndrome.periodontitis has been identified as a potential risk factor in cardiovascular diseases. it is possible that the stimulation of host responses to oral infections may result in vascular damage and the inducement of blood clotting. the aim of this study was to assess the role of periodontal infection and bacterial burden as an explanatory variable to the activation of the inflammatory process leading to acute coronary syndrome (acs).200616805672
reclassification of bacteroides distasonis, bacteroides goldsteinii and bacteroides merdae as parabacteroides distasonis gen. nov., comb. nov., parabacteroides goldsteinii comb. nov. and parabacteroides merdae comb. nov.the characteristics of three bacteroides species, bacteroides distasonis, bacteroides goldsteinii and bacteroides merdae, were examined. 16s rrna gene sequence analysis showed that b. distasonis, b. goldsteinii and b. merdae should not be classified as species within the genus bacteroides. although b. distasonis, b. goldsteinii and b. merdae were phylogenetically related to tannerella forsythensis, the ratios of anteiso-c(15 : 0) to iso-c(15 : 0) in whole-cell methanolysates of the three species ...200616825636
[prevalence of putative periodontal microorganisms in chinese patients with aggressive periodontitis].to evaluate the prevalence of putative periodontal microorganism in chinese patients with aggressive periodontitis (agp).200616836903
effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes.patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ...200616841550
inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study.inflammation is a significant component of atherosclerosis lesions. bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial groel with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis.200616842503
treponema denticola in disseminating endodontic infections.treponema denticola is a consensus periodontal pathogen that has recently been associated with endodontic pathology. in this study, the effect of mono-infection of the dental pulp with t. denticola and with polymicrobial "red-complex" organisms (rc) (porphyromonas gingivalis, tannerella forsythia, and t. denticola) in inducing disseminating infections in wild-type (wt) and severe-combined-immunodeficiency (scid) mice was analyzed. after 21 days, a high incidence (5/10) of orofacial abscesses was ...200616861296
sampling strategy for intraoral detection of periodontal pathogens before and following periodontal therapy.the aim of this study was to identify a sampling strategy with high probability for detecting oral colonization by actinobacillus actinomycetemcomitans, porphyromonas gingivalis, eikenella corrodens, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, and treponema denticola before and following mechanical periodontal therapy.200616881801
antimicrobial therapy using a local drug delivery system (arestin) in the treatment of peri-implantitis. i: microbiological outcomes.to assess the microbiological outcome of local administration of minocycline hydrochloride microspheres 1 mg (arestin) in cases with peri-implantitis and with a follow-up period of 12 months.200616907769
association of eubacterium nodatum and treponema denticola with human periodontitis lesions.the purpose of the present investigation was to compare the levels, proportions and percentage of sites colonized by 40 bacterial species in subgingival plaque samples from periodontally healthy subjects and patients with chronic periodontitis to seek possible pathogens other than the consensus pathogens porphyromonas gingivalis and tannerella forsythia.200616922925
porphyromonas gingivalis vesicles enhance attachment, and the leucine-rich repeat bspa protein is required for invasion of epithelial cells by "tannerella forsythia".the human oral cavity harbors more than 500 species of bacteria. periodontitis, a bacterially induced inflammatory disease that leads to tooth loss, is believed to result from infection by a select group of gram-negative periodontopathogens that includes porphyromonas gingivalis, treponema denticola, and "tannerella forsythia" (opinion on name change from tannerella forsythensis pending; formerly bacteroides forsythus). epithelial cell invasion by periodontopathogens is considered to be an impor ...200616926393
tannerella forsythia, a periodontal pathogen entering the genomic era. 200616930308
microbiota of the dorsum of the tongue after plaque accumulation: an experimental study in humans.the purpose of the present investigation was to determine the effect of the absence of tongue hygiene on the microbiota of the dorsum of the tongue.200616945032
levels of matrix metalloproteinases-8 and -9 with simultaneous presence of periodontal pathogens in gingival crevicular fluid as well as matrix metalloproteinase-9 and cholesterol in blood.to investigate the levels of matrix metalloproteinase (mmp) -8 and -9 with the simultaneous presence of periodontal pathogens in gingival crevicular fluid (gcf) as well as mmp-9 and cholesterol in blood. although bacterial pathogens are required to initiate the periodontal disease process, in some individuals the reaction to bacteria may lead to an excessive host response, resulting in a general inflammatory response.200616953818
periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases.report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis.200616970621
changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement.dental plaque is organized in a biofilm complex that provides protection and nutrients for periodontopathic bacteria. several factors can affect microbial colonization, including restorations and orthodontic brackets. the aim of this study was to investigate changes in subgingival microbiota and clinical parameters before and after bracket placement.200616979483
molecular analysis of filifactor alocis, tannerella forsythia, and treponema denticola associated with primary endodontic infections and failed endodontic treatment.the aim of this study was to investigate the presence of strict anaerobes such as filifactor alocis, tannerella forsythia, and treponema denticola in primary and secondary root-infected canals with periapical lesions by molecular analysis and the association of these species with specific endodontic signs and symptoms. microbial samples were taken from 100 root canals, 50 with necrotic pulp tissues (npt, primary infection), and 50 with failed endodontic treatment (fet, secondary infection). dna ...200616982268
denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology.bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. the aim of the present study was to show the potential of denaturing gradient gel electrophoresis (dgge) as a tool for the detection of clinically relevant species and to compare the results of detection by dgge with those by pcr and culturing. hybridization of the bands from the dgge profiles with species-specific probes was developed ...200617021091
synovial inflammation in active rheumatoid arthritis and psoriatic arthritis facilitates trapping of a variety of oral bacterial dnas.to investigate the presence of oral bacterial dnas in serum and synovial fluid (sf) of patients with active rheumatoid arthritis (ra) and psoriatic arthritis (psa).200617207381
rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease.one of the predominant polymicrobial infections of humans is expressed clinically as periodontal disease. porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly implicated as members of a pathogenic consortium in the etiology of adult periodontitis. in this study we hypothesized that p. gingivalis, t. denticola, and t. forsythia are synergistic in terms of virulence potential and induce chronic periodontal inflammation that leads to alveolar bone resorption in ...200717210663
inflammation associated with implants with different surface types.the aim of this study was to determine the nature of the inflammatory infiltrate associated with different transmucosal implant surfaces in dogs.200717224032
tannerella forsythensis prth genotype and association with periodontal status.the prth gene of tannerella forsythensis encodes for a cysteine protease possessing virulent properties. subgingival colonization by t. forsythensis with this genotype has been suggested to be a discriminator between periodontal health and disease. this study examined the prevalence of t. forsythensis prth genotype in subgingival plaque and its association with periodontal disease progression and current disease status.200717274725
phase-variable expression of a family of glycoproteins imparts a dynamic surface to a symbiont in its human intestinal ecosystem.the recent report of the synthesis of glycoproteins by the abundant intestinal symbionts bacteroides showed that these organisms use a novel bacterial enzyme to decorate their surfaces with a sugar residue derived from their environment. as a first step in understanding the importance of these glycoproteins to the bacteria and to the bacterial-host symbiosis, we identified and characterized the abundant glycoproteins of bacteroides distasonis (proposed reclassification as parabacteroides distaso ...200717284602
periodontal and oral microbiological status of an adult population undergoing haemodialysis: a cross-sectional study.the aim of this cross-sectional study was to evaluate the periodontal status and oral microbiological patterns of a population with end-stage renal disease (esrd), undergoing haemodialysis (hd).200717305623
microbiological outcomes of quadrant versus full-mouth root planing as monitored by real-time pcr.to study the short-term microbiological changes following full-mouth compared with quadrant wise scaling and root planing (fmrp and qrp) as well as long-term effects.200717309590
subgingival plaque microbiota in hiv positive patients.to describe and compare the predominant bacterial and fungal species associated with gingivitis, periodontitis, and linear gingival erythema (lge), in hiv positive subjects with different immune status.200717309593
a comparison of implant-supported, bar- or ball-retained mandibular overdentures: a retrospective clinical, microbiologic, and immunologic study of 10 edentulous patients attending a recall visit.clinical, microbiologic, and immunologic comparisons of the peri-implant health in edentulous volunteers wearing long-standing implant-supported ball- or dolder bar-retained mandibular overdentures were performed.200717319360
loss of adherence ability to human gingival epithelial cells in s-layer protein-deficient mutants of tannerella forsythensis.tannerella forsythensis, one of the important pathogens in periodontal disease, has a typical surface layer (s-layer) consisting of regularly arrayed subunits outside the outer membrane. the s-layer in t. forsythensis is suggested to be associated with haemagglutinating activity, adhesion and invasion of host cells; however, its precise functions have been unknown. orfs encoding the major s-layer proteins (230 and 270 kda) of t. forsythensis atcc 43037, tfsa and tfsb, respectively, following the ...200717322207
subgingival chlorhexidine varnish administration as an adjunct to same-day full-mouth root planing. ii. microbiological observations.recently, the clinical additive value of a highly concentrated chlorhexidine (chx) varnish, when applied subgingivally as an adjunct to scaling and root planing, was described. the objective of the present study was to investigate the microbiological impact of a treatment strategy for chronic periodontitis based on a combination of same-day full-mouth root planing and subgingival chx varnish administration.200717335366
the detection of eight putative periodontal pathogens in adult and rapidly progressive periodontitis patients: an institutional study.periodontal disease is a commonly prevalent problem faced alike by both the developed and third world countries but showing wide variations in prevalence and severity across different geographical areas. the purpose was to identify actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), ekinella corrodens (ec), campylobacter rectus (cr), bacteroides forsythus (bf), treponema denticola (td) and fusobacterium nucleatum (fn) in indian adult periodontiti ...200717347537
role of a tannerella forsythia exopolysaccharide synthesis operon in biofilm development.tannerella forsythia is a gram-negative oral anaerobe implicated in the development of periodontitis, a chronic inflammatory disease induced by bacterial infections which leads to tooth loss if untreated. since biofilms formed by periodontal bacteria are considered important in disease progression and pose difficulties in treatment, we sought to investigate the underlying mechanisms of t. forsythia biofilm formation. this was carried out by screening random insertion mutants of t. forsythia for ...200717363213
[prevalence of tannerella forsythensis in subgingival microflora of chinese chronic periodontitis patients].the aim of this study was to investigate the prevalence of t. forsythensis in chinese chronic periodontitis patients, and correlate it with clinical parameters.200717375576
demographic, clinical, and microbial aspects of chronic and aggressive periodontitis in colombia: a multicenter study.the microbial profile of periodontal disease varies among different human populations. this study evaluated the demographic, clinical, and microbiologic aspects of periodontitis in a multigeographic sample in colombia.200717397309
evidence of periopathogenic microorganisms in placentas of women with preeclampsia.periodontal disease is a chronic inflammatory infectious disease that may act as a focus of infection. preeclampsia is a pregnancy-specific hypertensive disorder that often leads to maternal morbidity and mortality. acute atherosis, the placental lesion of preeclampsia, shares many histopathological features with atherosclerosis. recently, chronic infection was linked to the initiation of atherosclerosis. oral pathogens have been detected in atherosclerotic plaques, where they may play a role in ...200717397314
correlation between atherosclerosis and periodontal putative pathogenic bacterial infections in coronary and internal mammary arteries.chronic infections, such as periodontitis, have been associated with an increased risk for atherosclerosis and coronary artery disease. the aim of this study was to investigate biopsy samples of coronary and internal mammary arteries for the presence of putative pathogenic bacteria (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, and tannerella forsythensis), chlamydia pneumoniae, and human cytomegalovirus (cmv).200717397315
occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a colombian population.differences in the composition of subgingival microbiota in periodontitis have been observed in different geographic locations. some of these microbial differences could have clinical significance. this study describes clinical parameters and the composition of the subgingival microbiota in chronic periodontitis (cp) and aggressive periodontitis (agp) patients in a colombian population.200717397318
aggregatibacter actinomycetemcomitans as indicator for aggressive periodontitis by two analysing strategies.to compare the subgingival microbiota of aggressive and chronic periodontitis (chp) using single-site and pooled plaque samples.200717433043
identification of intracellular oral species within human crevicular epithelial cells from subjects with chronic periodontitis by fluorescence in situ hybridization.interactions between oral bacteria and gingival epithelial cells play an important role in the pathogenesis of periodontal diseases. this study used in situ hybridization with 16 rrna probes and confocal microscopy to detect the periodontal pathogens porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythia, and treponema denticola within epithelial cells from periodontal pockets, gingival crevice, and buccal mucosa collected from subjects with chronic periodontitis (n ...200717451543
gene polymorphisms and the prevalence of key periodontal pathogens.growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. the aim of this project was to study whether gene polymorphisms of inflammatory markers are associated with the presence of viable periodontopathogenic bacteria. we extracted genomic dna from 45 young adults diagnosed with generalized aggressive periodontitis to study fc receptors, formyl peptide receptor, interleukin-6, tumor necrosis factor-alpha, and vitamin d receptor polymorphis ...200717452560
periodontal pathogens in periodontal pockets and in carotid atheromatous plaques.in the last years the relationship between periodontitis and cardiovascular diseases has been a subject of increasing research. the identification of periodontopathic bacteria in atheromas can contribute to our knowledge of such an association. the aim of our study was to assess the concomitant presence of 5 periodontal pathogens (actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, treponema denticola, and tannerella forsythia) in periodontal pockets and in car ...200717452955
persistently high levels of periodontal pathogens associated with preterm pregnancy outcome.few studies examining the association between periodontal diseases and preterm birth have explored the underlying microbial and antibody responses associated with oral infection.200717470016
periopathogenic bacteria in persistent periapical lesions: an in vivo prospective study.the aim of this in vivo prospective study was to detect the presence of periopathogenic bacteria in persistent periapical lesions and to compare the reliability of two different methods: anaerobic culture and the dna hybridization technique.200717470025
comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction.the outcome of microbiological diagnostics may depend on the sampling technique. it was the aim of the present study to compare two widely used sampling techniques for subgingival bacteria using quantitative real-time polymerase chain reaction.200717470026
activation of toll-like receptors 2 and 4 by gram-negative periodontal bacteria.periodontitis is a chronic infectious disease associated with a gram-negative subgingival microflora. bacterial components stimulate, among other receptors, toll-like receptor (tlr) 2 and/or tlr4. accumulating evidence indicates that both qualitatively and quantitatively distinct immune responses result from the triggering of tlr2 as compared to tlr4 triggering. the aim was to study the interaction of porphyromonas gingivalis, actinobacillus actinomycetemcomitans, tannerella forsythensis, prevot ...200717488439
identification of periodontopathic bacteria in gingival tissue of japanese periodontitis patients.the identification of invading periodontopathic bacteria in tissues is important to determine their role in the pathogenesis of periodontal disease. the objective of this study was to identify periodontopathic bacteria in diseased gingival tissue of periodontitis patients.200717488447
bacterial colonization immediately after installation on oral titanium implants.information on bacterial colonization immediately after dental implant insertion is limited.200717501978
bacterial species in subgingival plaque and oral bone loss in postmenopausal women.oral bacteria are widely recognized in the etiology of periodontal disease. we investigated the prevalence of subgingival bacterial infection with eight species, tested associations between infection and oral bone loss, and assessed potential confounding factors and effect modifiers of those associations in a large community-based cohort of postmenopausal women.200717539719
[subgingival microbiota in chronic and aggressive periodontitis in bogotá, colombia: an epidemiological approach].periodontitis-associated microbiotas differ in regions around the world and vary with a variety of factors. this suggests that each country must establish its own dental microbiobiotic profile in order to formulate adequate prevention measures and more specific treatments.200717546221
microbiological findings after periodontal therapy using curettes, er:yag laser, sonic, and ultrasonic scalers.to evaluate and compare the microbiological effects of hand instruments, er:yag-laser, sonic, and ultrasonic scalers in patients with chronic periodontitis. patient perception of each treatment was documented.200717555412
[pcr detection of periodontal bacteria and streptococcus mutans in the biological samples from the oral cavity].the paper describes an optimized technique for dna isolation from the streptococcus mutans cultures and from the clinical samples including proteinase k treatment and detergent lysis, followed by sorbent-based enrichment. this technique was employed for isolating dna from the periodontal bacteria porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and bacteroides forsythus. a multiplex pcr technique was adapted for large-scale studies of clinical samples. an original pcr method was d ...200717561665
population-based study of salivary carriage of periodontal pathogens in adults.large, general population-based data on carriage rates of periodontal pathogens hardly exist in the current literature. the objectives of the present study were to examine the salivary detection of aggregatibacter (formerly actinobacillus) actinomycetemcomitans, campylobacter rectus, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, and treponema denticola in a representative sample of the adult population living in southern finland and to clarify which determinants are a ...200717567788
periodontal pathogens in erupting third molars of periodontally healthy subjects.the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia and tannerella forsythensis in bacteriologic samples of 5-7-mm deep mandibular third-molar pericoronal pockets was analysed by polymerase chain reaction, to test the hypothesis that these sites would harbour the bacteria. the patients were periodontally healthy 20-year-old finnish male conscripts. sixteen had acute pericoronitis, 28 chronic pericoronitis, and 15 were symptom-free controls. a. ac ...200717629461
targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr.an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ...200717653931
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