Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| residue val237 is critical for the enantioselectivity of penicillium expansum lipase. | the shape of the hydrophobic tunnel leading to the active site of penicillium expansum lipase (pel) was redesigned by single-point mutations, in order to better understand enzyme enantioselectivity towards naproxen. a variant with a valine-to-glycine substitution at residue 237 exhibited almost no enantioselectivity (e = 1.1) compared with that (e = 104) of wild-type pel. the function of the residue, val237, in the hydrophobic tunnel was further analyzed by site-directed mutagenesis. for each of ... | 2014 | 24338160 |
| description of the mechanisms underlying geosmin production in penicillium expansum using proteomics. | a 2d-dige proteomics experiment was performed to describe the mechanism underlying the production of geosmin, an earthy-smelling sesquiterpene which spoils wine, produced by penicillium expansum. the strains were identified by sequencing of the its and beta-tubulin regions. this study was based on a selection of four strains showing different levels of geosmin production, assessed by gc-ms/ms. the proteomics study revealed the differential abundance of 107 spots between the different strains; th ... | 2014 | 24189443 |
| accumulation of the mycotoxin patulin in the presence of gluconic acid contributes to pathogenicity of penicillium expansum. | penicillium expansum, the causal agent of blue mold rot, causes severe postharvest fruit maceration through secretion of d-gluconic acid (gla) and secondary metabolites such as the mycotoxin patulin in colonized tissue. gla involvement in pathogenicity has been suggested but the mechanism of patulin accumulation and its contribution to p. expansum pathogenicity remain unclear. the roles of gla and patulin accumulation in p. expansum pathogenicity were studied using i) glucose oxidase gox2-rnai m ... | 2014 | 24024763 |
| growth parameters of penicillium expansum calculated from mixed inocula as an alternative to account for intraspecies variability. | the aim of this work was to compare the radial growth rate (μ) and the lag time (λ) for growth of 25 isolates of penicillium expansum at 1 and 20 ºc with those of the mixed inoculum of the 25 isolates. moreover, the evolution of probability of growth through time was also compared for the single strains and mixed inoculum. working with a mixed inoculum would require less work, time and consumables than if a range of single strains has to be used in order to represent a given species. suitable pr ... | 2014 | 25033331 |
| effects of the mycotoxin patulin at the level of nuclear receptor transcriptional activity and steroidogenesis in vitro. | patulin (pat) is a mycotoxin produced by various species of fungi, with penicillium expansum being the most commonly occurring. apples and apple products are the main sources of pat contamination. this mycotoxin has been shown to induce toxic effects in animals, a few of which include reproductive toxicity and interference with the endocrine system. here the endocrine disrupting potential of pat has been investigated in vitro to identify disruption at the level of oestrogen, androgen, progestage ... | 2014 | 24995434 |
| draft genome sequence of penicillium expansum strain r19, which causes postharvest decay of apple fruit. | among the species that cause blue mold, isolates of penicillium expansum are the most prevalent and virulent species, causing more than 50 percent of postharvest decay. we report the draft genome sequence of p. expansum r19 in order to identify fungal virulence factors and to understand the mechanism of infection. | 2014 | 24948776 |
| γ-aminobutyric acid induces resistance against penicillium expansum by priming of defence responses in pear fruit. | the results from this study showed that treatment with γ-aminobutyric acid (gaba), at 100-1000 μg/ml, induced strong resistance against blue mould rot caused by penicillium expansum in pear fruit. moreover, the activities of five defence-related enzymes (including chitinase, β-1,3-glucanase, phenylalnine ammonialyase, peroxidase and polyphenol oxidase) and the expression of these corresponding genes were markedly and/or promptly enhanced in the treatment with gaba and inoculation with p. expansu ... | 2014 | 24767023 |
| inhibitory effect of selenium against penicillium expansum and its possible mechanisms of action. | some organic and inorganic salts could inhibit the growth of many pathogens. selenium (se), as an essential micronutrient, was effective in improving the plant resistance and antioxidant capacity at a low concentration. penicillium expansum is one of the most important postharvest fungal pathogens, which can cause blue mold rot in various fruits and vegetables. in this study, the inhibitory effect of se against p. expansum was evaluated. the result showed that se strongly inhibited spore germina ... | 2014 | 24682262 |
| mechanism of penicillium expansum in response to exogenous nitric oxide based on proteomics analysis. | penicillium expansum is an important fungal pathogen, which causes blue mold rot in various fruits and produces a mycotoxin (patulin) with potential damage to public health. here, we found that nitric oxide (no) donor could significantly inhibit germinability of p. expansum spores, resulting in lower virulence to apple fruit. based on two dimension electrophoresis (2-de) and mass spectrometry (ms) analysis, we identified ten differentially expressed proteins in response to exogenous no in p. exp ... | 2014 | 24675182 |
| acidification of apple and orange hosts by penicillium digitatum and penicillium expansum. | new information about virulence mechanisms of penicillium digitatum and penicillium expansum could be an important avenue to control fungal diseases. in this study, the ability of p. digitatum and p. expansum to enhance their virulence by locally modulating the ph of oranges and apples was evaluated. for each host, ph changes with a compatible pathogen and a non-host pathogen were recorded, and the levels of different organic acids were evaluated to establish possible relationships with host ph ... | 2014 | 24667317 |
| effects of chitosan from cunninghamella elegans on virulence of post-harvest pathogenic fungi in table grapes (vitis labrusca l.). | this study aimed to obtain chitosan (chi) from cunninghamella elegans cultivated in corn step liquid (csl)-based medium under optimized conditions and to assess the efficacy of the obtained chi in inhibiting botrytis cinerea and penicillium expansum in laboratory media and when applied as a coating on table grapes (vitis labrusca l.). moreover, the influence of chi-based coatings on several physical, physicochemical and sensory characteristics of the fruits during storage was assessed. according ... | 2014 | 24321603 |
| penicillium strains isolated from slovak grape berries taxonomy assessment by secondary metabolite profile. | the secondary metabolite profiles of microfungi of the genus penicillium isolated from samples of grape berries collected in two different phases during two vegetative seasons in slovakia is described to assess the taxonomy. three slovak vine regions have been selected for this study, based on their climatic differences and national economic importance. cultures of microfungi isolated from berries were incubated on different selective media for macro and micromorphology identification. the speci ... | 2014 | 25109845 |
| antifungal activity of salicylic acid against penicillium expansum and its possible mechanisms of action. | apple is a fruit widely produced and consumed around the world. blue mold (penicillium expansum) is one of the main postharvest diseases in apples, leading to a wide use of fungicides and the search for alternative products. the aim of this study was to assess the effect of salicylic acid (sa) against p. expansum, elucidating its mechanisms of action. the antimicrobial effect was determined by exposing conidia to a 2.5 mm sa solution for 0 to 120 min, followed by incubation. the effect of ph on ... | 2015 | 26340673 |
| alkaloids with cardiovascular effects from the marine-derived fungus penicillium expansum y32. | three new alkaloids (1, 4 and 8), together with nine known analogues (2, 3, 5-7, and 9-12), were isolated from the marine-derived fungus penicillium expansum y32. their structures including the absolute configurations were elucidated by spectroscopic and mosher's and marfey's methods, along with quantum electronic circular dichroism (ecd) calculations. each of the compounds was evaluated for cardiovascular effects in a live zebrafish model. all of the compounds showed a significant mitigative ef ... | 2015 | 26506361 |
| the ability of a cold-adapted rhodotorula mucilaginosa strain from tibet to control blue mold in pear fruit. | cold-adapted yeasts were isolated from soil samples collected in tibet and evaluated as potential biocontrol agents against blue mold (penicillium expansum) of pear fruit in cold storage. yc1, an isolate identified as rhodotorula mucilaginosa, was found to exhibit the greatest biocontrol activity among the different isolates that were screened. a washed cell suspension of yc1 exhibited the best biocontrol activity among three different preparations that were used in the current study. a concentr ... | 2015 | 26454432 |
| modelling the effect of essential oil of betel leaf (piper betle l.) on germination, growth, and apparent lag time of penicillium expansum on semi-synthetic media. | the current study aimed at characterizing the chemical components of betel leaf (piper betle l. var. tamluk mitha) essential oil (bleo) and modelling its effect on growth of penicillium expansum on semi-synthetic medium. gas chromatography-mass spectrophotometry (gc-ms) analysis of bleo revealed the presence of different bioactive phenolic compounds in significant amounts. among 46 different components identified, chavibetol (22.0%), estragole (15.8%), β-cubebene (13.6%), chavicol (11.8%), and c ... | 2015 | 26439423 |
| contrasting genomic diversity in two closely related postharvest pathogens: penicillium digitatum and penicillium expansum. | penicillium digitatum and penicillium expansum are two closely related fungal plant pathogens causing green and blue mold in harvested fruit, respectively. the two species differ in their host specificity, being p. digitatum restricted to citrus fruits and p. expansum able to infect a wide range of fruits after harvest. although host-specific penicillium species have been found to have a smaller gene content, it is so far unclear whether these different host specificities impact genome variation ... | 2015 | 26672008 |
| phytic acid enhances biocontrol activity of rhodotorula mucilaginosa against penicillium expansum contamination and patulin production in apples. | the effect of rhodotorula mucilaginosa in combination with phytic acid (pa) on blue mold decay and patulin contamination of apples was investigated. results from this study show that different concentrations of pa were effective in reducing the disease incidence of apples and that pa at concentration of 4 μmol/ml, decreased the incidence of blue mold decay in apples from 86.1 to 62.5%, and showed higher control efficacy compared to untreated, control fruit during storage at 20°c. however, r. muc ... | 2015 | 26635764 |
| penicillium species present in uruguayan salami. | the surface coverage of certain dry fermented sausages such as italian salami by some species of penicillium provides their characteristic flavor and other beneficial properties. one of them is the protective effect by means of a uniform film of white mold against undesirable microorganisms. the aim of this work was to identify and to isolate the fungal species present in mature italian type of salami and to evaluate if it is possible to obtain some of them as starters. in addition, the effects ... | 2015 | 22610286 |
| characterization of the 5' flanking region of lipase gene from penicillium expansum and its application in molecular breeding. | a major challenge for further promotion of lipase productivity in penicillium expansum pe-12 is to find a suitable promoter that can function efficiently in this industrial strain. in this study, the 5' flanking region of p. expansum lipase (ppel) containing a putative novel promoter sequence was characterized by fusing to β-glucuronidase (gus) and subsequently introducing into p. expansum. as a result, all the transformants showed blue color quickly after incubation in gus detection buffer, sug ... | 2015 | 24502561 |
| in vitro antifungal activity of allyl isothiocyanate (aitc) against aspergillus parasiticus and penicillium expansum and evaluation of the aitc estimated daily intake. | isothiocyanates (itcs) are natural compounds derived from cruciferous vegetables produced by enzymatic conversion of metabolites called glucosinolates. they are potentially useful antimicrobial compounds for food applications have been shown to be promising agents against cancer in human cell culture, animal models, and in epidemiological studies. in this study, the antifungal activity of the allyl isothiocyanate (aitc) was evaluated on two mycotoxigenic fungi as aspergillus parasiticus and peni ... | 2015 | 26140951 |
| crocin prevents patulin-induced acute toxicity in cardiac tissues via the regulation of oxidative damage and apoptosis. | patulin (pat) is a mycotoxin produced by several species of the genera of penicillium, aspergillus, and byssochlamys principally by penicillium expansum. this mycotoxin is suspected to affect several organs including kidney and liver. however, its toxic effect on heart remains unknown. the present study investigated for the first time the cardiotoxic effect of pat in mice. we demonstrated that pat increased creatinin phosphokinase (cpk) level, induced lipoperoxydation and protein oxidation, and ... | 2015 | 26095701 |
| kinetics of biofilm formation by drinking water isolated penicillium expansum. | current knowledge on drinking water (dw) biofilms has been obtained mainly from studies on bacterial biofilms. very few reports on filamentous fungi (ff) biofilms are available, although they can contribute to the reduction in dw quality. this study aimed to assess the dynamics of biofilm formation by penicillium expansum using microtiter plates under static conditions, mimicking water flow behaviour in stagnant regions of drinking water distribution systems. biofilms were analysed in terms of b ... | 2015 | 26010032 |
| effects of carbon, nitrogen and ambient ph on patulin production and related gene expression in penicillium expansum. | patulin, a potent mycotoxin which can cause serious health concerns, is mainly produced in foods by penicillium expansum. environmental factors play important roles in regulating biosynthesis of mycotoxins; however, information about the effects of environmental factors on patulin production and the involved mechanisms in p. expansum is limited. here, we investigated the effects of different carbon (c) and nitrogen (n) sources, and ambient ph on patulin production in three p. expansum strains t0 ... | 2015 | 26001378 |
| development of a real-time pcr assay for penicillium expansum quantification and patulin estimation in apples. | due to the occurrence and spread of the fungal contaminants in food and the difficulties to remove their resulting mycotoxins, rapid and accurate methods are needed for early detection of these mycotoxigenic fungi. the polymerase chain reaction and the real time pcr have been widely used for this purpose. apples are suitable substrates for fungal colonization mostly caused by penicillium expansum, which produces the mycotoxin patulin during fruit infection. this study describes the development o ... | 2015 | 25998812 |
| evaluation of yeasts from tibetan fermented products as agents for biocontrol of blue mold of nashi pear fruits. | a total of 20 strains of yeast isolated from tibetan fermented products were screened for antagonism against blue mold of pear caused by penicillium expansum. six isolates that inhibited incidence of postharvest decay by 35% or more were selected for further screening. among them, the most effective was rhodotorula mucilaginosa. the results showed that washed cell suspensions of r. mucilaginosa yielded better antagonistic efficacy than unwashed cell-culture mixtures, cell-free culture filtrates, ... | 2015 | 25845361 |
| highly efficient and regioselective synthesis of dihydromyricetin esters by immobilized lipase. | dihydromyricetin is the principle component of the chinese herbal tea teng-cha and a promising ingredient for functional food and nutraceuticals, but its low solubility limits its application potentials. this study explored enzymatic acylation of dihydromyricetin to improve its solubility in lipid systems. acylation was achieved with several lipases with the synthesis of a major (>86%) product and a minor product. isolation and purification of the products by preparative hplc followed by lc-ms, ... | 2015 | 25701177 |
| effect of the yeast rhodosporidium paludigenum on postharvest decay and patulin accumulation in apples and pears. | the effect of a strain of marine yeast rhodosporidium paludigenum on postharvest blue mold and patulin accumulation in apples and pears stored at 23°c was evaluated. the occurrence and severity of apple and pear decay caused by penicillium expansum were significantly inhibited by r. paludigenum. however, the application of the yeast at a high concentration (10(8) cells per ml) enhanced patulin accumulation after 7 days of storage; the amount of patulin increased 24.2 times and 12.6 times compare ... | 2015 | 25581191 |
| development of a novel quantitative pcr assay as a measurement for the presence of geosmin-producing fungi. | to provide an efficient technique for monitoring the off-flavoured fungal compound geosmin. | 2015 | 25580564 |
| elucidation of the concise biosynthetic pathway of the communesin indole alkaloids. | the communesins are a prominent class of indole alkaloids isolated from penicillium species. owing to their daunting structural framework and potential as pharmaceuticals, communesins have inspired numerous synthetic studies. however, the genetic and biochemical basis of communesin biosynthesis has remained unexplored. herein, we report the identification and characterization of the communesin (cns) biosynthetic gene cluster from penicillium expansum. we confirmed that communesin is biosynthesiz ... | 2015 | 25571861 |
| poly(-β-hydroxybutyrate) (phb) depolymerase phaz pen from penicillium expansum: purification, characterization and kinetic studies. | very few studies have been dedicated to r-hydroxyacids (r-ha) production using extracellular polyhydroxyalkanoate depolymerases (ephazs). penicillium expansum produced maximum extracellular polyhydroxybutyrate depolymerase (~6 u/ml) by 72 h when grown in mineral salt medium containing 0.2 % w/v phb, ph 5.0, at 30 °c and 200 rpm shaking conditions. partial purification of the extracellular poly(-β-hydroxybutyrate) depolymerase phaz pen from p. expansum by two steps using ammonium sulphate (80 % s ... | 2015 | 28324398 |
| characterizing the proteome and oxi-proteome of apple in response to a host (penicillium expansum) and a non-host (penicillium digitatum) pathogen. | apples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. to obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed by reactive oxygen species (ros) in 'golden smoothee' apple in response to wounding, penicillium expansum (host) and penicillium digitatum (non-host) pathogens with select transcriptional studies ... | 2015 | 25464364 |
| mechanism of h₂o₂-induced oxidative stress regulating viability and biocontrol ability of rhodotorula glutinis. | the use of antagonistic yeasts to control postharvest pathogens is a promising alternative to fungicides. the effectiveness of the antagonists against fungal pathogens is greatly dependent on their viability, which is usually mediated by reactive oxygen species (ros). here, we investigated the effects of h₂o₂-induced oxidative stress on the viability and biocontrol efficacy of rhodotorula glutinis and, using flow cytometric analysis, observed the changes of ros accumulation and apoptosis in the ... | 2015 | 25462935 |
| genome, transcriptome, and functional analyses of penicillium expansum provide new insights into secondary metabolism and pathogenicity. | the relationship between secondary metabolism and infection in pathogenic fungi has remained largely elusive. the genus penicillium comprises a group of plant pathogens with varying host specificities and with the ability to produce a wide array of secondary metabolites. the genomes of three penicillium expansum strains, the main postharvest pathogen of pome fruit, and one pencillium italicum strain, a postharvest pathogen of citrus fruit, were sequenced and compared with 24 other fungal species ... | 2015 | 25338147 |
| the effect of chitosan and whey proteins-chitosan films on the growth of penicillium expansum in apples. | penicillium expansum causes a major post-harvest disease of apples. the aim of this study was to investigate the inhibition effect of chitosan and whey proteins-chitosan films containing different amounts of quince and cranberry juice against p. expansum on the simulation medium and on apples. the mechanical properties of films were also evaluated. | 2015 | 25074824 |
| antifungal effect of gamma irradiation and sodium dichloroisocyanurate against penicillium expansum on pears. | gamma irradiation (gi) was evaluated for its in vitro and in vivo antifungal activity against penicillium expansum on pear fruits. gi showed a complete inhibition of spore germination, germ tube elongation and mycelial of p. expansum, especially 1·8 kgy. gi affected the membrane integrity and cellular leakage of conidia in a dose-dependent manner. furthermore, the leakage of protein and sugar from mycelia increased along with the dose. gi was evaluated at lower doses in combination with a chlori ... | 2015 | 26174206 |
| antifungal activity of essential oils against selected terverticillate penicillia. | the aim of this study was to screen 15 essential oils of selected plant species, viz. lavandula angustifolia, carum carvi, pinus mungo var. pulmilio, mentha piperita, chamomilla recutita l., pinus sylvestris, satureia hortensis l., origanum vulgare l., pimpinella anisum, rosmarinus officinalis l., salvia officinalis l., abietis albia etheroleum, chamomilla recutita l. rausch, thymus vulgaris l., origanum vulgare l. for antifungal activity against five penicillium species: penicillium brevicompac ... | 2015 | 25780826 |
| changes in flavour and microbial diversity during natural fermentation of suan-cai, a traditional food made in northeast china. | we measured changes in the main physical and chemical properties, flavour compounds and microbial diversity in suan-cai during natural fermentation. the results showed that the ph and concentration of soluble protein initially decreased but were then maintained at a stable level; the concentration of nitrite increased in the initial fermentation stage and after reaching a peak it decreased significantly to a low level by the end of fermentation. suan-cai was rich in 17 free amino acids. all of t ... | 2015 | 26159472 |
| [antagonistic activity of bacillus amyloliquefaciens subsp. plantarum imv b-7404 and bim b-439d strains towards pathogenic bacteria and micromycetes]. | in this study the antagonistic activity of strains bacillus amyloliquefaciens subsp. plantarum imv b-7404 and bim b-439d against bacterial and fungal pathogens of agricultural crops has been researched. it is shown that both strains of bacilli demonstrated a high level of antagonism to the vascular bacteriosis pathogen, average level of antagonism to micromycetes--root rot pathogens. to ofiobulez pathogen strain b. amyloliquefaciens subsp. plantarum bim b-439d was more active. cultural liquid of ... | 2015 | 25639040 |
| effects of ionizing radiation on postharvest fungal pathogens. | postharvest diseases cause losses in a wide variety of crops around the world. irradiation, a useful nonchemical approach, has been used as an alternative treatment for fungicide to control plant fungal pathogens. for a preliminary study, ionizing radiations (gamma, x-ray, or e-beam irradiation) were evaluated for their antifungal activity against botrytis cinerea, penicillium expansum, and rhizopus stolonifer through mycelial growth, spore germination, and morphological analysis under various c ... | 2015 | 26060436 |
| ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit. | hot water treatment (hwt) of fruit is an effective approach for managing postharvest decay of fruits and vegetables. in the present study, the effects of hwt (45 °c for 10 min) on the growth of botrytis cinerea and penicillium expansum in vitro, and gray (b. cinerea) and blue mold (p. expansum) development in kiwifruit were investigated. hwt effectively inhibited spore germination and germ tube elongation of b. cinerea and p. expansum. reactive oxygen species accumulation and protein impairment ... | 2015 | 26002370 |
| coatings comprising chitosan and mentha piperita l. or mentha × villosa huds essential oils to prevent common postharvest mold infections and maintain the quality of cherry tomato fruit. | in this study, we evaluated the efficacy of coatings comprising shrimp chitosan (chi) and mentha piperita l. (mpeo) or mentha × villosa huds (mveo) essential oils to control mold infections caused by aspergillus niger, botrytis cinerea, penicillium expansum and rhizopus stolonifer in cherry tomato fruits (solanum lycopersicum l.) during storage at room temperature (25°c for 12 days) and low temperature (12°c for 24 days). the effects of the coatings on the physicochemical and sensory characteris ... | 2015 | 26313246 |
| mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes. | in the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ... | 2015 | 26178241 |
| antifungal activity of violacein purified from a novel strain of chromobacterium sp. niist (mtcc 5522). | a novel strain of chromobacterium sp. niist (mtcc 5522) producing high level of purple blue bioactive compound violacein was isolated from clay mine acidic sediment. during 24 h aerobic incubation in modified luria bertani medium, around 0.6 g crude violacein was produced per gram of dry weight biomass. an inexpensive method for preparing crystalline, pure violacein from crude pigment was developed (12.8 mg violacein/l) and the pure compound was characterized by different spectrometric methods. ... | 2015 | 26428920 |
| control of household mycoflora in fermented sausages using phenolic fractions from olive mill wastewaters. | biopreservation using polyphenols represents an alternative to chemical molecules for improving food safety. in this work, we evaluated the antifungal activity of polyphenols extracted from olive mill wastewater (omwwp) to reduce or eliminate the growth of undesired fungi on the surface of dry fermented sausages. antagonism against penicillium expansum dsmz 1282, penicillium verrucosum dsmz 12639, penicillium nalgiovense ms01, aspergillus ochraceus dsmz 63304, cladosporium cladosporioides ms12, ... | 2015 | 25996624 |
| control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. | the aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (ddhr) on a wide range of fungal pathogens and its potential to control plant fungal diseases. | 2015 | 25810243 |
| arginine acts as an inhibitor of the biosynthesis of several mycotoxins. | it is well known that the type and the availability of nitrogen have a great influence on the biosynthesis of certain mycotoxins. here it is shown that some amino acids have no influence, some others strongly support and a third group inhibits the biosynthesis of ochratoxin (ota) by penicillium nordicum even in a complex medium, such as pda. arginine (arg) is one of the strong ota inhibiting amino acids. it was shown that arg not only inhibits ota in penicillium but also citrinin (cit) biosynthe ... | 2016 | 27400452 |
| use of oil-in-water emulsions to control fungal deterioration of strawberry jams. | this work aimed to control the fungal deterioration of strawberry jams. the antifungal activity of the clove, cinnamon leaf, lemon and mandarin essential oils and their effectiveness in oil-in-water emulsions were evaluated. according to the results obtained, only clove and cinnamon leaf oils were selected to prepare emulsions. all the tested emulsions were stable, independently the amount of polymer and essential oil used. essential oil loss was affected by the amount of polymer employed to pre ... | 2016 | 27283611 |
| antimicrobial activity of new 2-thioxo-benzo[g]quinazolin-4(3h)-one derivatives. | the antimicrobial activity of a synthesized series of 28 2-thioxo-benzo[g]quinazolin-4(3h)-one derivatives was evaluated in vitro against five gram-positive bacteria, including bacillus subtilis, enterococcus faecalis, staphylococcus aureus, staphylococcus epidermidis and streptococcus pyogenes. the antibacterial activity was extended to include five gram-negative bacteria: pseudomonas aeruginosa, escherichia coli, proteus mirabilis, klebsiella oxytoca and enterobacter cloacae. furthermore, the ... | 2016 | 27306606 |
| antimicrobial activity of synthesized 2-methylthiobenzo[g][1,2,4]- triazolo[1,5-a]quinazoline derivatives. | the present study was carried out to evaluate the antimicrobial activity of a synthesized 2-methylthio-benzo[g][1,2,4]triazolo[1,5- a]quinazoline series. the compounds (1-21) were tested against a variety of gram-positive bacterial species including bacillus subtilis (rcmb 01001 69-3), enterococcus faecalis (rcmb 0100154-2), staphylococcus aureus (rcmb 0100183-9), staphylococcus epidermidis (rcmb 0100183-9) and streptococcus pyogenes (rcmb 0100172-5). in addition, gram-negative bacteria were als ... | 2016 | 27140181 |
| horizontal acquisition of toxic alkaloid synthesis in a clade of plant associated fungi. | clavicipitaceae is a fungal group that comprises species that closely interact with plants as pathogens, parasites or symbionts. a key factor in these interactions is the ability of these fungi to synthesize toxic alkaloid compounds that contribute to the protection of the plant host against herbivores. some of these compounds such as ergot alkaloids are toxic to humans and have caused important epidemics throughout history. the gene clusters encoding the proteins responsible for the synthesis o ... | 2016 | 26688468 |
| green synthesis of nanosilver particles by aspergillus terreus ha1n and penicillium expansum ha2n and its antifungal activity against mycotoxigenic fungi. | the aim of this study was to synthesize silver nanoparticles (agnps) by an eco-friendly and low-cost method using the fungi aspergillus terreus ha1n and penicillium expansum ha2n as an alternative to chemical procedures mostly requiring drastic experimental conditions emitting toxic chemical byproducts. also, this study has been extended to evaluate the effect of agnps on the growth of some mycotoxigenic fungi and ochratoxin a (ota) produced by aspergillus ochraceus. | 2016 | 27002915 |
| carbon regulation of environmental ph by secreted small molecules that modulate pathogenicity in phytopathogenic fungi. | fruit pathogens can contribute to the acidification or alkalinization of the host environment. this capability has been used to divide fungal pathogens into acidifying and/or alkalinizing classes. here, we show that diverse classes of fungal pathogens-colletotrichum gloeosporioides, penicillium expansum, aspergillus nidulans and fusarium oxysporum-secrete small ph-affecting molecules. these molecules modify the environmental ph, which dictates acidic or alkaline colonizing strategies, and induce ... | 2016 | 26666972 |
| in vitro study of the antifungal activity of essential oils obtained from mentha spicata, thymus vulgaris and laurus nobilis. | the aim of this study was to determine the antifungal activity of the essential oils isolated from three aromatic plants against 13 filamentous fungal strains. the major constituents of mentha spicata, thymus vulgaris, and laurus nobilis essential oils were carvone (52.2%), linalool (78.1%), and 1,8-cineole (45.6%), respectively. m. spicata essential oil exhibited the strongest activity against all tested fungi in which fusarium graminearum, f.moniliforme, and penicillium expansum were the most ... | 2016 | 27697030 |
| screening for antimicrobial activity of wood rotting higher basidiomycetes mushrooms from uruguay against phytopathogens. | in this work, the antimicrobial activity of extracts of wood rotting higher basidiomycetes mushrooms isolated from eucalyptus plantations in uruguay was studied using bacterial and fungal phytopathogens as targets. fifty-one extracts from mycelia and growth broth were prepared from higher basidiomycetes mushrooms, from which eight extracts (from ganoderma resinaceum, laetiporus sulphureus, dictyopanus pusillus, and bjerkandera adusta) showed antimicrobial activity against xanthomonas vesicatoria ... | 2016 | 27481160 |
| biochemical characterization and antimicrobial and antifungal activity of two endemic varieties of garlic (allium sativum l.) of the campania region, southern italy. | extracts of the bulbs of the two endemic varieties "rosato" and "caposele" of allium sativum of the campania region, southern italy, were analyzed. the phenolic content, ascorbic acid, allicin content, and in vitro antimicrobial and antifungal activity were determined. ultra performance liquid chromatography with diode array detector performed polyphenol profile. the polyphenolic extracts showed antioxidant activity (ec50) lower than 120 mg. the amount of ascorbic acid and allicin in the two ext ... | 2016 | 27259073 |
| antifungal screening of lavender essential oils and essential oil constituents on three post-harvest fungal pathogens. | a growing body of literature indicates that many synthetic pesticides have adverse effects on human, animal, and environmental health. as a result, plant-derived natural products are quickly gaining momentum as safer and less ecologically damaging alternatives due to their low toxicity, high biodegradability, and good specificity. essential oils of lavandula angustifolia, lavandula x intermedia cv grosso, and lavandida x intermedia cv provence as well as various mono- and sesquiterpene essential ... | 2016 | 27396210 |
| yeasts isolated from figs (ficus carica l.) as biocontrol agents of postharvest fruit diseases. | fresh fruit is highly perishable during postharvest life, mainly due to fungal growth. thus, fungal control is an important goal for the fruit industry. in this work, a selection of antagonistic yeasts isolated from fig and breba crops were screened in vitro. the isolated yeasts were challenged with three moulds isolated from decayed figs and breba crops, identified as penicillium expansum m639 and cladosporium cladosporioides m310 and m624, and pathogenic moulds botrytis cinerea cect20518 and m ... | 2016 | 27052701 |
| quantification and identification of microorganisms found on shell and kernel of fresh edible chestnuts in michigan. | chestnut is a relatively new cultivated crop for michigan, and postharvest loss due to decay has been problematic as production has increased each year. in 2007, more than 25% of chestnuts were lost to postharvest decay, equivalent to approximately 5300 kg of fresh product. to determine the organisms responsible for decay, a microbiological survey was performed in 2006 and 2007 to identify microorganisms involved in postharvest shell (external surface) mold and internal kernel (edible portion) d ... | 2016 | 26869338 |
| development of a dna microarray-based assay for the detection of sugar beet root rot pathogens. | sugar beet root rot diseases that occur during the cropping season or in storage are accompanied by high yield losses and a severe reduction of processing quality. the vast diversity of microorganism species involved in rot development requires molecular tools allowing simultaneous identification of many different targets. therefore, a new microarray technology (arraytube) was applied in this study to improve diagnosis of sugar beet root rot diseases. based on three marker genes (internal transc ... | 2016 | 26524545 |
| postharvest disease control of colletotrichum gloeosporioides and penicillium expansum on stored apples by gamma irradiation combined with fumigation. | to study the control of postharvest decay caused by colletotrichum gloeosporioides and penicillium expansum, gamma irradiation alone or in combination with fumigation was evaluated to extend the shelf life of apples in south korea. an irradiation dose of 2.0 kgy resulted in the maximum inhibition of c. gloeosporioides and p. expansum spore germination. the gamma irradiation dose required to reduce the spore germination by 90% was 0.22 and 0.35 kgy for c. gloeosporioides and p. expansum, respecti ... | 2016 | 27721696 |
| fungal and host transcriptome analysis of ph-regulated genes during colonization of apple fruits by penicillium expansum. | penicillium expansum is a destructive phytopathogen that causes decay in deciduous fruits during postharvest handling and storage. during colonization the fungus secretes d-gluconic acid (gla), which modulates environmental ph and regulates mycotoxin accumulation in colonized tissue. till now no transcriptomic analysis has addressed the specific contribution of the pathogen's ph regulation to the p. expansum colonization process. for this purpose total rna from the leading edge of p. expansum-co ... | 2016 | 27146851 |
| screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of brazil. | lactic acid bacteria (lab) can be isolated from different sources such as milk and cheese, and the lipolytic, proteolytic and glycolytic enzymes of lab are important in cheese preservation and in flavour production. moreover, lab produce several antimicrobial compounds which make these bacteria interesting for food biopreservation. these characteristics stimulate the search of new strains with technological potential. from 156 milk and cheese samples from cow, buffalo and goat, 815 isolates were ... | 2016 | 26608755 |
| factors affecting the content of the ursolic and oleanolic acid in apple peel: influence of cultivars, sun exposure, storage conditions, bruising and penicillium expansum infection. | for health promotion and as part of natural plant protection, it might be of interest to increase the content of oleanolic acid (oa) and ursolic acid (ua) by biofortification in breeding programs, although the extent of the influence of genetic and environmental factors needs to be clarified. the objective of this study was to determine the effect of cultivar, sun exposure, storage, bruising and fungal infection on the oa and ua content in apple peel. | 2016 | 26147234 |
| molecular characterization of patulin producing and non-producing penicillium species in apples from morocco. | the isolation of patulin-producing penicillia in apples collected in different markets in four localities in morocco is reported. fungi were identified by β-tubulin sequencing and further characterized using a specific pcr-based method targeting the isoepoxydon dehydrogenase (idh) gene to discriminate between patulin-producing and non-producing strains. production of patulin was also evaluated using standard cultural and biochemical methods. results showed that 79.5% of contaminant fungi belonge ... | 2016 | 26513254 |
| patulin transformation products and last intermediates in its biosynthetic pathway, e- and z-ascladiol, are not toxic to human cells. | patulin is the main mycotoxin contaminating apples. during the brewing of alcoholic beverages, this mycotoxin is degraded to ascladiol, which is also the last precursor of patulin. the present study aims (1) to characterize the last step of the patulin biosynthetic pathway and (2) to describe the toxicity of ascladiol. a pate deletion mutant was generated in penicillium expansum. in contrast to the wild strain, this mutant does not produce patulin but accumulates high levels of e-ascladiol with ... | 2016 | 27913847 |
| laea regulation of secondary metabolism modulates virulence in penicillium expansum and is mediated by sucrose. | penicillium expansum, the causal agent of blue mould rot, is a critical health concern because of the production of the mycotoxin patulin in colonized apple fruit tissue. although patulin is produced by many penicillium species, the factor(s) activating its biosynthesis are not clear. sucrose, a key sugar component of apple fruit, was found to modulate patulin accumulation in a dose-responsive pattern. an increase in sucrose culture amendment from 15 to 175 mm decreased both patulin accumulation ... | 2016 | 27528575 |
| tissue oxidative stress induced by patulin and protective effect of crocin. | patulin (pat) is a secondary toxic metabolite produced principally by penicillium expansum. this mycotoxin is known to be teratogenic, mutagenic, immunotoxic and neurotoxic, and it has been shown to cause damage in several organs in laboratory animals. this study focuses on the prevention of experimental murine pat-induced nephrotoxicity and hepatotoxicity. we investigate the ability of a natural product, crocin (cro), to counteract the toxic effects of pat. pre-treatment of mice with cro preven ... | 2016 | 26584762 |
| patulin is a cultivar-dependent aggressiveness factor favouring the colonization of apples by penicillium expansum. | the blue mould decay of apples is caused by penicillium expansum and is associated with contamination by patulin, a worldwide regulated mycotoxin. recently, a cluster of 15 genes (pata-pato) involved in patulin biosynthesis was identified in p. expansum. blast analysis revealed that patl encodes a cys6 zinc finger regulatory factor. the deletion of patl caused a drastic decrease in the expression of all pat genes, leading to an absence of patulin production. pathogenicity studies performed on 13 ... | 2016 | 26582186 |
| a study on the physicochemical parameters for penicillium expansum growth and patulin production: effect of temperature, ph, and water activity. | penicillium expansum is among the most ubiquitous fungi disseminated worldwide, that could threaten the fruit sector by secreting patulin, a toxic secondary metabolite. nevertheless, we lack sufficient data regarding the growth and the toxigenesis conditions of this species. this work enables a clear differentiation between the favorable conditions to the p. expansum growth and those promising for patulin production. a mathematical model allowing the estimation of the p. expansum growth rate acc ... | 2016 | 27386110 |
| metabolomic and transcriptomic comparison of solid-state and submerged fermentation of penicillium expansum kacc 40815. | penicillium spp. are known to harbor a wide array of secondary metabolites with cryptic bioactivities. however, the metabolomics of these species is not well-understood in terms of different fermentation models and conditions. the present study involved metabolomics profiling and transcriptomic analysis of penicillium expansum 40815 under solid-state fermentation (ssf) and submerged fermentation (smf). metabolite profiling was carried out using ultra-performance liquid chromatography quadruple t ... | 2016 | 26863302 |
| efficacy of salicylic acid to reduce penicillium expansum inoculum and preserve apple fruits. | apples are among the most commonly consumed fruits worldwide. blue mold (penicillium expansum) is one of the major diseases in apples postharvest, leading to wide use of fungicides and the search for alternative products to control the pathogen. in this context, this study aimed to evaluate the potential of salicylic acid (sa) as an alternative product to control blue mold and to preserve the physicochemical characteristics of apple fruit postharvest. the antimicrobial effect of sa was determine ... | 2016 | 26808096 |
| isoepoxydon dehydrogenase (idh) gene expression in relation to patulin production by penicillium expansum under different temperature and atmosphere. | penicillium expansum growth and patulin production occur mainly at post-harvest stage during the long-term storage of apples. low temperature in combination with reduced oxygen concentrations is commonly applied as a control strategy to extend apple shelf life and supply the market throughout the year. our in vitro study investigated the effect of temperature and atmosphere on expression of the idh gene in relation to the patulin production by p. expansum. the idh gene encodes the isoepoxydon de ... | 2016 | 26788884 |
| post-harvest proteomics of grapes infected by penicillium during withering to produce amarone wine. | the study of withered grape infection by penicillium, a potentially toxigenic fungus, is relevant to preserve grape quality during the post-harvest dehydration process. this report describes the first proteomic analysis of amarone wine grapes, infected by two strains of penicillium expansum (pe1) and penicillium crustosum (pc4). protein identification by ms analysis allowed a better understanding of physiological mechanisms underlying the pathogen attack. the pe1 strain had a major impact on vit ... | 2016 | 26776019 |
| ammonia activates pacc and patulin accumulation in an acidic environment during apple colonization by penicillium expansum. | penicillium expansum, the causal agent of blue mould rot, causes severe post-harvest fruit maceration simultaneously with the secretion of d-gluconic acid (gla) and the mycotoxin patulin in colonized tissue. the factor(s) inducing patulin biosynthesis during colonization of the host acidic environment is unclear. during the colonization of apple fruit in vivo and growth in culture, p. expansum secretes ph-modulating gla and ammonia. although patulin and its possible opportunistic precursor gla a ... | 2016 | 26420024 |
| pre-harvest application of oxalic acid increases quality and resistance to penicillium expansum in kiwifruit during postharvest storage. | kiwifruit (actinidia deliciosa cv. bruno) fruits were sprayed with 5mm oxalic acid (oa) at 130, 137, and 144 days after full blossom, and then harvested at commercial maturity [soluble solid content (ssc) around 10.0%] and stored at room temperature (20 ± 1 °c). pre-harvest application of oa led to fruit with higher ascorbic acid content at harvest, slowed the decreases in fruit firmness and ascorbic acid content and increase in ssc during storage, and also decreased the natural disease incidenc ... | 2016 | 26213007 |
| transcriptomic responses of the basidiomycete yeast sporobolomyces sp. to the mycotoxin patulin. | patulin is a mycotoxin produced by penicillium expansum, the causal agent of blue mold of stored pome fruits, and several other species of filamentous fungi. this mycotoxin has genotoxic, teratogenic and immunotoxic effects in mammals, and its presence in pome fruits and derived products represents a serious health hazard. biocontrol agents in the pucciniomycotina, such as the yeasts sporobolomyces sp. strain iam 13481 and rhodosporidium kratochvilovae strain ls11, are able to resist patulin and ... | 2016 | 26956724 |
| biocontrol activity and patulin-removal effects of bacillus subtilis, rhodobacter sphaeroides and agrobacterium tumefaciens against penicillium expansum. | this study was conducted to evaluate the biocontrol potential of bacillus subtilis cicc 10034, rhodobacter sphaeroides cgmcc 1.2182 and agrobacterium tumefaciens cgmcc 1.2554 against patulin (pat)-producer penicillium expansum and their ability to remove pat. | 2016 | 27328641 |
| penicillium expansum link strain for a biometallurgical method to recover rees from weee. | due to the wide range of applications in high-tech solutions, rare earth elements (rees) have become object of great interest. in the last years several studies regarding technologies for ree extraction from secondary resources have been carried out. in particular biotechnologies, which use tolerant and accumulator microorganisms to recover and recycle precious metals, are replacing traditional methods. this paper describes an original biometallurgical method to recover rees from waste electrica ... | 2017 | 27520390 |
| effects of the protective, curative, and eradicative applications of chitosan against penicillium expansum in apples. | apple is one of the most important temperate fruit to brazil economy, and the use of synthetic chemicals has been the main method for reducing postharvest diseases, such as the blue mold, caused by penicillium expansum. this work intends to evaluate the practical utilization of chitosan for blue mold control. for this purpose, fruits were treated in a preventive and curative way, immersing the fruits in chitosan solution (5 or 10mgml(-1)), or adding a single drop of this solution (10mgml(-1)) di ... | 2017 | 27515465 |
| comparison of the effects of three types of aminobutyric acids on the control of penicillium expansum infection in pear fruit. | aminobutyric acids were reported to have inhibitory effects on postharvest fungal diseases. the effects of α-aminobutyric acid (aaba), β-aminobutyric acid (baba) and γ-aminobutyric acid (gaba) on the control of penicillium expansum infection in pear fruit were compared in the present study. | 2017 | 27392194 |
| combined effect of antagonistic yeast and modified atmosphere to control penicillium expansum infection in sweet cherries cv. ambrunés. | fruit decay caused by pathogenic moulds is a major concern in the postharvest quality and shelf life of fruit. blue mould decay is caused by penicillium expansum (p. expansum) and is one of the most important postharvest diseases in cherries (prunus avium l.). synthetic fungicides are the main medium used to control pathogenic moulds. however, alternative approaches are available for developing safer technologies to control postharvest disease. an integrated approach that combines biological con ... | 2017 | 27821356 |
| temperature, water activity and ph during conidia production affect the physiological state and germination time of penicillium species. | conidial germination and mycelial growth are generally studied with conidia produced under optimal conditions to increase conidial yield. nonetheless, the physiological state of such conidia most likely differs from those involved in spoilage of naturally contaminated food. the present study aimed at investigating the impact of temperature, ph and water activity (aw) during production of conidia on the germination parameters and compatible solutes of conidia of penicillium roqueforti and penicil ... | 2017 | 27780083 |
| bergamotane sesquiterpenes with alpha-glucosidase inhibitory activity from the plant pathogenic fungus penicillium expansum. | two new bergamotane sesquiterpene lactones, named expansolides c and d (1 and 2), together with two known compounds expansolides a and b (3 and 4), were isolated from the plant pathogenic fungus penicillium expansum accc37275. the structures of the new compounds were established by detailed analyses of the spectroscopic data, especially 1d-, 2d-nmr, and hr-esi-ms. in an in vitro bioassay, the epimeric mixture of expansolides c and d (1 and 2) (in a ratio of 2:1 at the temprature of the bioassay) ... | 2017 | 27582055 |
| essential oil composition and antifungal activity of melissa officinalis originating from north-est morocco, against postharvest phytopathogenic fungi in apples. | to investigate biological control methods against post-harvest phytopathogenic fungi in apples, tests on the antifungal activity of essential oil of melissa officinalis were carried out. the essential oil, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (gc-ms). analysis of the essential oil was able to detect 88.7% of the components. the main components are p-mentha-1,2,3-triol (13.1%), p-menth-3-en-8-ol (8.8%), pulegone (8.8%), piperitynone oxid ... | 2017 | 28389346 |
| pcppi: a comprehensive database for the prediction of penicillium-crop protein-protein interactions. | penicillium expansum , the causal agent of blue mold, is one of the most prevalent post-harvest pathogens, infecting a wide range of crops after harvest. in response, crops have evolved various defense systems to protect themselves against this and other pathogens. penicillium -crop interaction is a multifaceted process and mediated by pathogen- and host-derived proteins. identification and characterization of the inter-species protein-protein interactions (ppis) are fundamental to elucidating t ... | 2017 | 28365721 |
| secondary metabolism in penicillium expansum: emphasis on recent advances in patulin research. | the plant pathogenic fungus penicillium expansum is a major concern of the global food industry due to its wide occurrence and ability to produce various mycotoxins, of which the most significant is patulin. relatively less highlighted in the literature, in comparison with the other food-borne mycotoxins, patulin is one of the main factors in economic losses of vegetables and fruits. otherwise, patulin is a health hazard which results in both short-term and long-term risks. this review includes ... | 2017 | 28362209 |
| evaluation of penicillium expansum for growth, patulin accumulation, nonvolatile compounds and volatile profile in kiwi juices of different cultivars. | patulin (pat) contamination and changes of nonvolatile and volatile compounds caused by penicillium expansum in fresh juices of 8 kiwi cultivars were investigated. growth and pat production of p. expansum were greater at 25°c than at 4°c. p. expansum grew in kiwi juices under ph ranging from 2.75 to 3.27 and produced 45.10-268.88μg/ml of pat at 25°c. decreases occurred in malic acid and soluble protein; while consumption and production simultaneously happened for reducing sugar, total soluble so ... | 2017 | 28317715 |
| genotyping-by-sequencing markers facilitate the identification of quantitative trait loci controlling resistance to penicillium expansum in malus sieversii. | blue mold caused by penicillium expansum is the most important postharvest disease of apple worldwide and results in significant financial losses. there are no defined sources of resistance to blue mold in domesticated apple. however, resistance has been described in wild malus sieversii accessions, including plant introduction (pi)613981. the objective of the present study was to identify the genetic loci controlling resistance to blue mold in this accession. we describe the first quantitative ... | 2017 | 28257442 |
| genome sequencing and analysis of the postharvest fungus penicillium expansum r21. | blue mold is the vernacular name of a common postharvest disease of stored apples, pears, and quince that is caused by several common species of penicillium this study reports the draft genome sequence of penicillium expansum strain r21, which was isolated from a red delicious apple in 2011 in pennsylvania. | 2017 | 28209811 |
| patulin degradation by the biocontrol yeast sporobolomyces sp. is an inducible process. | patulin is a mycotoxin produced by penicillium expansum and a common contaminant of pome fruits and their derived products worldwide. it is considered to be mutagenic, genotoxic, immunotoxic, teratogenic and cytotoxic, and the development of strategies to reduce this contamination is an active field of research. we previously reported that sporobolomyces sp. is able to degrade patulin and convert it into the breakdown products desoxypatulinic acid and ascladiol, both of which were found to be le ... | 2017 | 28208615 |
| dry formulations of the biocontrol agent candida sake cpa-1 using fluidised bed drying to control the main postharvest diseases on fruits. | the biocontrol agent candida sake cpa-1 is effective against several diseases. consequently, the optimisation of a dry formulation of c. sake to improve its shelf life and manipulability is essential for increasing its potential with respect to future commercial applications. the present study aimed to optimise the conditions for making a dry formulation of c. sake using a fluidised bed drying system and then to determine the shelf life of the optimised formulation and its efficacy against penic ... | 2017 | 28111760 |
| the response of growth and patulin production of postharvest pathogen penicillium expansum to exogenous potassium phosphite treatment. | in this study, the effects of exogenous potassium phosphite (phi) on growth and patulin production of postharvest pathogen penicillium expansum were assessed. the results indicated that p. expansum under 5mmol/l phi stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple fruit. meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that phi mainly affected the early steps of patulin synthetic route at transcription ... | 2017 | 28042969 |
| proteome changes in penicillium expansum grown in a medium derived from host plant. | penicillium expansum causes blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. however, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. in this work, a two-dimensional gel electrophoresis-based proteomic approach was applied to probe changes in p. expansum 3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. the results showed that the ph value a ... | 2017 | 27994211 |
| production of reuterin in a fermented milk product by lactobacillus reuteri: inhibition of pathogens, spoilage microorganisms, and lactic acid bacteria. | we assessed the antimicrobial activity of reuterin produced in vitro in glycerol aqueous solutions in situ by lactobacillus reuteri atcc 53608 as part of a fermented milk product against starter (lactobacillus delbrueckii ssp. bulgaricus and streptococcus thermophilus), spoilage (penicillium expansum), pathogenic (staphylococcus aureus, salmonella enterica ssp. enterica, and listeria monocytogenes), and pathogen surrogate (escherichia coli dh5α) microorganisms. we also assayed the influence of c ... | 2017 | 28342608 |
| effects and possible mechanism of tea tree oil against botrytis cinerea and penicillium expansum in vitro and in vivo test. | the purpose of this study was to investigate the antifungal activities and possible mechanisms of tea tree oil (tto) against botrytis cinerea and penicillium expansum in vitro and in vivo. the results show that tto exhibits dose-dependent antifungal activity against both pathogens, but p. expansum is less sensitive than b. cinerea to tto not only in the in vitro test but also in artificially inoculated cherry fruits. tto vapor treatment reduced the decay caused by these pathogens in inoculated c ... | 2017 | 28177805 |
| influence of high power ultrasound on selected moulds, yeasts and alicyclobacillus acidoterrestris in apple, cranberry and blueberry juice and nectar. | the purpose of this study was to investigate the effect of non-thermal technology, high power ultrasound (hpu) on inactivation of aspergillus ochraceus 318, penicillium expansum 565, rhodotorula sp. 74, saccharomyces cerevisiae 5 and alicyclobacillus acidoterrestris dsm 3922 in clear juices and nectars from apple, blueberry and cranberry juice concentrate. inoculated juice and nectars were treated by high power ultrasound (20khz) according to procedure set by central composite design (ccd). thre ... | 2017 | 28242037 |
| chemical variability, antioxidant and antifungal activities of essential oils and hydrosol extract of calendula arvensis l. from western algeria. | the chemical composition of the essential oils and hydrosol extract from aerial parts of calendula arvensis l. was investigated using gc-fid and gc/ms. intra-species variations of the chemical compositions of essential oils from 18 algerian sample locations were investigated using statistical analysis. chemical analysis allowed the identification of fifty-three compounds amounting to 92.3-98.5% with yields varied of 0.09-0.36% and the main compounds were zingiberenol 1 (8.7-29.8%), eremoligenol ... | 2017 | 28109063 |
| influence of structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. | the aim of this article is influence of the structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. this work was based on the general remark that even the smallest change in the structure of a compound can affect its biological properties. the results of the biotransformation of four bicyclic unsaturated lactones with one or two methyl groups in the cyclohexene ring was tested using fifteen fungal strains (fusarium spe ... | 2017 | 28107178 |
| enhancing melting curve analysis for the discrimination of loop-mediated isothermal amplification products from four pathogenic molds: use of inorganic pyrophosphatase and its effect in reducing the variance in melting temperature values. | loop-mediated isothermal amplification (lamp) is widely used for differentiating causative agents in infectious diseases. melting curve analysis (mca) in conjunction with the lamp method reduces both the labor required to conduct an assay and contamination of the products. however, two factors influence the melting temperature (tm) of lamp products: an inconsistent concentration of mg(2+) ion due to the precipitation of mg2p2o7, and the guanine-cytosine (gc) content of the starting dumbbell-like ... | 2017 | 27984058 |