Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine s + l-cells. | 1976 | 57666 | |
| spontaneous release of endogenous ecotropic type c virus from rat embryo cultures. | type c viruses were isolated from embryo cultures of two different rat strains, sprague-dawley and fischer. both viruses (termed rat leukemia virus, ralv) were released spontaneously from rat embryo cells, have a density of 1.14 to 1.15 g/cm(3) based on equilibrium sedimentation in sucrose gradients, contain 60-70s rna, rna-directed dna polymerase, and rat type c virus-specific 30,000 molecular-weight-protein determinants. molecular hybridization studies using the sprague-dawley ralv 60-70s rna ... | 1976 | 58077 |
| sequence homology between moloney murine sarcoma virus and moloney leukemia virus rna. | the moloney murine sarcoma-leukemia virus [m-msv (mulv)], propagated at high multiplicity of infection (moi), was demonstrated previously to contain a native genome mass of 4 x 10(6) daltons as contrasted to a mass of 7 x 10(6) daltons for moloney murine leukemia virus (m-mulv). the 4 x 10(6)-dalton classof rna from m-msv (mulv) was examined for base sequence homology with dna complementary to the 7 x 10(6)-dalton m-mulv rna genome. approximately 86% of the m-msv (mulv) was protected from rnase ... | 1976 | 59817 |
| immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. | the immunological properties of two murine thymus-dependent (t) lymphocyte surface glycoproteins, t200 and t25, were investigated. t200 is a lymphocyte-specific antigen with a high degree of species specificity. it shares antigenic determinants with molecules present on thymus-independent (b) lymphocytes. t25 has antigenic determinants which cross-react with antigens on mouse brain, rat thymocytes and rat brain. an antiserum against a purified rat brain glycoprotein which carries thy-1.1 reacts ... | 1976 | 61878 |
| revertants of human cells transformed by murine sarcoma virus. | revertants of nonproducer human osteosarcoma (np/khos) cells induced by kirsten murine sarcoma virus were isolated after incubating at high temperature (40.5 degrees c) overnight and subcloning at 36 degrees c. the morphologic variants, from which murine sarcoma virus could no longer be rescued, had growth properties similar to those of the nontransformed, parent human osteosarcoma cells and did not release rna-dependent dna polymerase activity. these revertants were nontumorigenic in nude mice. ... | 1976 | 62397 |
| 'glioblastoma'. induction of a reproducible autochonous tumor in rats with murine sarcoma virus. | inoculation of 0.02 ml of high-titer kirsten strain murine sarcoma virus into the brains of 10-day-old wistar/furth rats yields, with 100 percent incidence, a uniform glioblastoma-like tumor within 16 days. light and electronmicroscopy confirmed the neuroectodermal origin of the parenchymal cells. the remarkable vascular component was studied with extracellular tracers. the permeability of the abnormal endothelium to constituents of the blood vascular compartment was confirmed. accessory vascula ... | 1976 | 174026 |
| a comparison of membrane glycoconjugates from mouse cells transformed by murine and primate rna sarcoma viruses. | kirsten murine sarcoma virus (ki-msv) transformed balb/et3 mouse cells (k-balb) were found to have altered membrane glycoconjugates compared to normal balb/3t3 cells. there were reduced amounts of mono- and disialogangliosides, gm1 and gd1a, and activity of the specific galactosyltransferase required for synthesis of these gangliosides was reduced to between 0 and 18.5% of normal in the several k-balb clones examined. when fucose-labeled glycopeptides derived from the surfaces of balb/3t3 and k- ... | 1976 | 174712 |
| altered microfilament structure in cells transformed with a temperature-sensitive transformation mutant of murine sarcoma virus. | the structure and distribution of microfilaments were examined by electron microscopy in uninfected normal rat kidney (nrk) cells, murine sarcoma virus (msv)-transformed nrk cells, and nrk cells infected with a cold-sensitive transformation mutant of msv, i.e., nrk (msv-1b) cells, grown at both permissive (39 degrees) and nonpermissive (33 degrees) temperature. the uninfected cells contained numerous microfilaments which were especially prominent at sites of intercellular adherens junctions. in ... | 1976 | 174810 |
| recent studies on the identification of proliferative abnormalities and of oncogenic potential of cutaneous cells in individuals at increased risk of colon cancer. | this recent study describes the growth characteristics of acr skin fibroblasts in culture and their differential susceptibility to transformation by kirsten murine sarcoma virus (ki-msv). the sf were derived from normal appearing subepidermoid biopsies of acr individuals, their progeny and ocntrols. normal sf were contact-inhibited and grew only in 15% fcs. sf of acr subjects, and some asymptomatic acr progeny were not contact inhibited, grew in both 1% and 15% fcs and were considerably more sus ... | 1976 | 11561 |
| modification of the phenotype of murine sarcoma virus-transformed cells by sodium butyrate. effects on morphology and cytoskeletal elements. | 1976 | 185074 | |
| autogenous humoral immunity to baboon xenotropic endogenous type c virus. | a radioimmune precipitation assay was used to evaluate the humoral immune response of baboons (papio cynocephalus) to their endogenous, xenotropic, type c (m7) virus. using 125i or [3h]leucine-labeled intact virus preparation, we demonstrated that baboons possess naturally occurring virus-binding antibodies to this virus. antibody appeared directed against envelope and not internal viral proteins. immunoglobulins responsible for virus-binding were predominant in the igg and iga serum fractions, ... | 1976 | 185400 |
| cellular immunity to murine sarcoma virus-induced tumors as measured by macrophage migration inhibition assays. | c57bl/6n mice immunized with regressor murine sarcoma virus (musv) were studied at different times after inoculation for their cellular immune responses as measured by migration inhibition assays. we used both the direct capillary and indirect agarose dropiet methods and, as the source of antigens, viable tumor cells and 3 m kcl-solubilized extracts. migration inhibitory factor (mif) production coulctivity becoming undetectable after 21 days. however, the level of activity and the kinetics of pr ... | 1977 | 200758 |
| localization of meso-tetra(p-sulfophenyl)porphine in murine sarcoma virus-induced tumor-bearing mice. | meso-tetra(p-sulfophenyl)porphine (tpps4) has been found to accumulate in the tumors of mice bearing a murine sarcoma virus-induced rhabdomyosarcoma to a greater degree than in all other tissues except for the kidney. tumor to tissue ratios of from 3:1 (tumor to liver) to 9:1 (tumor to muscle) were observed. the uptake of tpps4 was found to be dose dependent, and the highest tumor to tissue ratios were found 96 hours after injection. the water-soluble tpps4 appears to be cleared through the kidn ... | 1977 | 201378 |
| suppressor cell regulation of immune response to tumors: abrogation by adult thymectomy. | the regulatory role of the adult thymus on the appearance of cytotoxic and suppressor t cells (thymus-derived lymphocytes) to allogeneic and autochthonous virus-induced tumors in mice was investigated. it was demonstrated that c57bl/6 mice challenged with allogeneic p815 mastocytoma cells and complete freund's adjuvant failed to develop cytotoxic cells but instead developed suppressor t cells which inhibited cytotoxic t cell function. further, adjuvant-induced suppressor cells prevented the prim ... | 1977 | 197528 |
| genetics of murine sarcoma virus (msv)--induced tumors in akr mice: evidence that late progressing and early regressing tumors are controlled by different gents. | the genetics of late appearing msv tumors showing a progressive growth pattern in akr mice was investigated. the late msv tumor response in f1 hybrids depended on the genetic background of the non-akr parent. within the 4-month observation period following virus injection, (cba x akr) f1, (dba/2 x akr)f1, and (nih x akr)f1 developed progressing msv tumors, which exhibited latency and growth behavior comparable to that seen in akr mice, (balb x akr)f1, (b6 x akr)f1, and (b10br x akr)f1 mice did n ... | 1977 | 66212 |
| the murine sarcoma virus-induced tumor: exception or general model in tumor immunology? | 1977 | 66859 | |
| search for oncogenic viruses in human prostate cancer. | electron microscopic, immunologic, and biochemical methods have been used in an attempt to detect and characterize oncornaviruses in human prostatic carcinoma (pca) and benign prostatic hyperplasia (bph), and in prostates of mice of high and low mammary cancer or leukemia strains. ultrastructural examination of 37 pca and nine bph specimens has revealed the presence of particles resembling type c virus in five cases of pca and one of bph, and also two different types of intracisternal virus-like ... | 1977 | 68817 |
| phosphorylation and nucleic acid binding properties of m1 moloney murine sarcoma virus-specific pp60gag. | the pp60gag polyprotein of the feline leukemia virus pseudotype of m1 moloney murine sarcoma virus [m1msv(felv)] was previously shown to be msv specific and to contain murine p30 and smaller structural polypeptides. this protein was detected in m1msv-transformed cells, and in pulse-chase studies it was found to be stable. in this study virion p60 was shown to contain murine pp12, to be phosphorylated, and to bind to nucleic acids. 32p-labeled m1msv[felv) was fractionated by guanidine agarose chr ... | 1977 | 69721 |
| isolation of a type c rna virus from an established human histiocytic lymphoma cell line. | a type c rna virus has been detected in the culture fluids of the su-dhl-1 human histiocytic lymphoma cell line previously established in this laboratory. in electron micrographs, the virus closely resembled other typical mammalian type c rna tumor viruses in size and morphology. viral rna-dependent dna polymerase activity has been demonstrated in particles (densities of 1.15 and 1.22 g/ml) in the microsomal cytoplasmic fraction and in pellets of culture fluids. the enzyme is partially inhibited ... | 1977 | 70039 |
| lack of expression of type c hamster virus after neoplastic transformation of hamster embryo fibroblasts by benzo(a)pyrene. | syrian hamster embryo fibroblasts transformed in vitro with benzo(a)pyrene were analyzed for the presence of type c viral components, including extra- and intracellular reverse transcriptase activity, intracellular type c hamster virus-related rna, and cellular hamster virus group-specific antigen. no evidence could be obtained for the presence of any of these components, although they were easily detectable in hamster fibroblasts producing either b-34 virus (a hamster virus pseudotype of harvey ... | 1977 | 71196 |
| heparan sulfates of mouse cells. analysis of parent and transformed 3t3 cell lines. | heparan sulfate from the surface of a variety of mouse cells at different cell densities was examined by ion-exchange chromatography. the results of this analysis show that: (1) the heparan sulfate from new isolates of swiss 3t3 cells transformed by sv40 virus (a dna tumor virus) elutes from deae-cellulose at a lower ionic strength than that from the parent cell type. this finding confirms our earlier observation with an established sv40-transformed cell line (underhill and keller, '75) and elim ... | 1977 | 137910 |
| interruption of oncornavirus replication by modified rifamycin antibiotics. | thirteen rifamycin sv derivatives containing 3'-alkylaminomethyl substituents fail to inhibit the activities of the simian sarcoma virus type 1 dna polymerase, and of cellular dna, rna, and poly(a) polymerases prepared from nih swiss mouse embryos. these compounds show a range in their toxicities for nih swiss mouse 3t3 cells and in their capacities to inhibit production of foci of morphologically altered cells by murine sarcoma virus (msv). three compounds--the n-methyl-n-hydroxyethylaminomethy ... | 1977 | 81643 |
| association of the glycolipid pattern with antigenic alterations in mouse fibroblasts transformed by murine sarcoma virus. | the level of the neutral glycolipid, galnac beta 1 leads to 4 gal beta 1 leads to 4glc leads to cer (asialo gm2), in balb/c 3t3 mouse fibroblasts transformed by kirsten murine sarcoma virus (3t3kimsv) was greatly increased compared to the nontransformed parental cells (3t3). this elevated chemical quantity was found to be localized on the surface of intact cells and accessible to external reagents, as detected by immunofluorescence and labeling with galactose oxidase: nab3h4. furthermore, immuni ... | 1977 | 192447 |
| murine sarcoma virus pseudotypes used as immunogens against viral and chemical oncogenesis. | the purpose of this study was to develop an animal system of protective immunity against oncornaviruses and to test whether such immunization had an inhibitory effect upon chemical sarcomagenesis. several murine sarcoma virus (msv) pseudotypes were used as immunogens and tested against themselves, against other pseudotypes, against leukemogenesis by their helper viruses, and against sarcomagenesis by 3-methylcholanthrene. five msv pseudotypes were obtained by rescuing complete msv from msv-genom ... | 1977 | 192461 |
| relationships between h-2 and viral antigens in murine oncornavirus-induced tumours. | cytolytic t lymphocytes (ctl) from murine sarcoma virus (msv) or friend leukaemia virus (flv) inoculated mice lyse syngeneic much more efficiently than allogeneic fmrgi+ lymphoma cells. by comparing the cytolysis of various h-2 different 51cr lymphomas by ctl from several inbred and congenic lines differing at h-2, and by competition experiments using unlabelled cells, one can demonstrate that this phenomenon is due to an h-2 barrier. h-2b/h-2d hybrid-anti-msv-ctl immunized by h-2b, h-2d or h-2b ... | 1977 | 192802 |
| genetic studies of the ploidy of moloney murine leukemia virus. | an assay for moloney murine leukemia virus was developed that made use of the production of morphologically altered foci in nonproducer mouse cells (15f) carrying murine sarcoma virus. wild-type (wt) virus gave a ratio of titers at 39 degrees c/34degrees c = 1.05 +/- 0.45 (standard deviation;n = 20). a spontaneous, thermosensitive (ts) mutant of moloney murine leukemia virus, ts3, defective in a late viral function, gave 39 degrees c/34degrees c = 0. a murine cell line (tb) was mixedly infected ... | 1977 | 192918 |
| different mechanisms for morphologic reversion of a clonal population of murine sarcoma virus-transformed nonproducer cells. | 1977 | 193260 | |
| growth of murine sarcoma virus-transformed rat kidney cells in nude mice: absence of induction of host endogenous viruses. | clonal isolates of the normal rat kidney cell line (nrk) transformed by a defective murine sarcoma virus (kirsten strain) were injected into nude mice of balb/c background to determine whether the growth of these cells as tumors was accompanied by the induction of host endogenous type c viruses. all the virus-transformed clones produced rapidly growing tumors in nude mice, but neither the induction of mouse endogenous viruses nor the rescue and spread of the transforming sarcoma virus were obser ... | 1977 | 194043 |
| feline oncornavirus-associated cell membrane antigen: expression in transformed nonproducer mink cells. | the feline oncornavirus-associated cell membrane antigen (focma) is a target for naturally occurring immunity that protects the cat against development of fibrosarcoma and leukemia. feline sarcoma virus-transformed "nonproducer" mink cells express high levels of focma, but not the major viral structural proteins. transformation of the same cells by murine sarcoma virus, or infection with feline leukemia virus, which is nontransforming for epithelial or fibroblastic cells, did not induce focma. t ... | 1977 | 194310 |
| inherited resistance to n- and b-tropic murine leukemia viruses in vitro. effect of the fv-1 locus on the rescue of a replication-defective and transformation-defective murine sarcoma virus in the fv-1 congenic strains sim.s and sim.r. | 1977 | 194408 | |
| in vitro translation of harvey murine sarcoma virus rna. | the viral rna of the harvey strain of murine sarcoma virus (ha-sv), which does not encode for any known viral structural polypeptides, has been translated in a nuclease-digested, cell-free system. the major protein product of the in vitro translation reaction has a molecular weight of 21,000 and is initiated faithfully with [35s]formylmethionine from formyl-[35s]methionyl-trnafmet. this polypeptide is clearly distinct from the rna of the moloney strain of type c helper virus used to pseudotype t ... | 1977 | 195086 |
| propagation of a b-tropic murine leukemia virus in n-type cells in the presence of different murine sarcoma viruses. | b-tropic mulv, wn1802b, was converted to nb-tropic after 5 days of propagation in its nonpermissive n-type mlg or c3h2k cells in the presence of the s + l - musv, but not in the presence of the harvey or kirsten strains of mssv. | 1977 | 195914 |
| characterization to effector lymphocytes associated with immunity to murine sarcoma virus (msv)-induced tumors. ii. repeated stimulation and proliferation in vitro of specific cytolytic t lymphocytes. | 1977 | 196025 | |
| exclusive involvement of h-2db or h-2kd product in the interaction between t-killer lymphocytes and syngeneic h-2b or h-2d viral lymphomas. | it was demonstrated previously that the cytolysis of murine viral lymphoma cells by anti-murine sarcoma virus (msv) syngeneic t-killer lymphocytes was restricted by some products of the h-2 complex. the respective role of the products of different regions of the h-2 complex were studied with six h-2(b) and three h-2(d) lymphomas induced by five different type c viruses. they were tested in a classical chromium release test against anti-msv t-killer cells obtained from different inbred strains of ... | 1977 | 197198 |
| isolation and preliminary characterization of temperature-sensitive mutants of the murine sarcoma leukaemia virus complex. | five temperature-sensitive mutants (ts i to 5) were isolated from a stock of the moloney strain of murine sarcoma leukaemia virus complex which had been mutagenized by ultraviolet irradiation or n-methyl-n'-nitro-n-nitrosoguanidine. in mouse cells at the non-permissive temperature the mutants formed fewer foci of transformed cells than at the permissive temperature. the ts mutants were characterized by testing: (i) murine leukaemia virus (mulv) clones from the ts complex, (2) the effect of addit ... | 1977 | 197202 |
| effect of helper virus on the number of murine sarcoma virus dna copies in infected mammalian cells. | cell lines of four mammalian species were each examined for the number of moloney murine sarcoma virus (m-msv) dna copies in total cellular dna after m-msv transformation. sarcoma-positive, leukemia-negative (s+l-) m-msv-transformed cells were compared to m-msv-transformed cells infected with a replicating leukemia virus. both unfractionated m-msv complementary dna and complementary dna representing the msv-specific and the msv-murine leukemia virus-common regions of the m-msv genome were hybrid ... | 1977 | 197257 |
| relationship between moloney murine leukemia and sarcoma virus rnas: purification and hybridization map of complementary dnas from defined regions of moloney murine sarcoma virus 124. | complementary dnas (cdna's) specific for various regions of the moloney murine sarcoma virus (msv) 124 rna genome were prepared by cross-hybridization techniques. a cdna specific for the first 1,000 nucleotides adjacent to the rna 3' end (cdna 3') was prepared and shown to also be complementary to the 3'-terminal 1,000 nucleotides of a related moloney murine leukemia virus (mlv) genome. a cdna complementary to the "msv-specific" portion of the msv 124 genome was prepared. this cdna was shown not ... | 1977 | 197259 |
| [rejection of non viral tumors caused by infection with murine sarcoma virus]. | 1977 | 197370 | |
| expression of murine sarcoma virus genes in uninfected rat cells subjected to anaerobic sress. | exposure of uninfected rat cells in tissue culture to anaerobic culture conditions induces transcription of rna corresponding to the two principal constituents of rat-derived type-c sarcoma virus genomes: (i) those specific rat cell sequences present in the kirsten and harvey murine sarcoma virus genomes, and (ii) an endogenous type-c rat leukemia virus. | 1977 | 197602 |
| transformation of rat liver epithelial cells by kirsten murine sarcoma virus. | we studied the transformation of epithelial, diploid cell lines (rl-33 and rl-34) derived from w rat liver by the kirsten murine sarcoma virus. on days 4-5 after virus infection, the epithelial cells began to pile up focally, forming small projections and releasing round cells from the foci. the epithelial cells grew in chains or as islets and grew in suspension above the cells attached to the bottom of the flasks when the cultures reached the confluent stage. the virus titration pattern was "o ... | 1977 | 198567 |
| characterization of gazdar murine sarcoma virus by nucleic acid hybridization and analysis of viral expression in cells. | gazdar murine sarcoma virus (gz-msv) and moloney murine sarcoma virus (m-msv) are closely related. the complete m-msv-specific nucleic acid sequences constituted a major portion of gz-msv-specific sequences. the msv-specific sequences in both gz-msv and m-msv genomes shared homology with hamster leukemia virus nucleic acid sequences. both rat cells (s+l+) and hamster (s+l-) cells expressed two viral proteins of 68,000 and 70,000 daltons. these proteins were immunologically related to p60 purifie ... | 1977 | 199741 |
| density-dependent changes in hexose transport, glycolytic enzyme levels, and glycolytic rates, in uninfected and murine sarcoma virus-transformed rat kidney cells. | 1977 | 201477 | |
| detection of human c-type "helper" viruses in human leukemic bone marrow with murine sarcoma virus-transformed human and rat non-producer cells. | bone-marrow cells from two leukemic children were co-cultivated with the leukemic children a 7573. in early passages, c-type oncornaviruses were released as detected by extracellular reverse transcriptase assay. co-cultivation of the infected canine cells with the non-producing cell lines r-970-5 (human) or k-nrk (rat) both transformed by kirsten mouse sarcoma virus (msv) yielded a new pseudotype of msv that could transform rat embryo, rabbit sirc and human kidney cells but not mouse embryo cell ... | 1977 | 188771 |
| transformation of cultured rat adrenocortical cells by kirsten murine sarcoma virus (ki-msv). | two-week-old primary cultures of normal adult rat adrenal cortex were exposed to kirsten murine sarcoma virus (kimsv). within a week, the adrenal cells, which are normally fusiform and aligned in parallel, became pleomorphic and piled up extensively. saturation density increased from 5-10 x 10(4) to 5-10 x 10(5) cells/cm2, population doubling time during exponential growth decreased from 36-40 to 16h, acid production increased and the growth rate became independent of a reduction in serum concen ... | 1977 | 188772 |
| rate of divergence of cellular sequences homologous to segments of moloney sarcoma virus. | the rna genome of the moloney isolate of murine sarcoma virus (m-msv) consists of two parts--a sarcoma-specific region with no homology to known leukemia viral rnas, and a shared region present also in moloney murine leukemia virus rna. complementary dna was isolated which was specific for each part of the m-msv genome. the dna of a number of mammalian species was examined for the presence of nucleotide sequences homologous with the two m-msv regions. both sets of viral sequences had homologous ... | 1977 | 189062 |
| standardization of the chromium-51 release, cell-mediated cytotoxicity assay: cryopreservation of mouse effector and target cells. | utilizing controlled cryopreservation techniques, we were able to standardize the 51cr release cytotoxicity assay and thereby ensured reliable comparisons between results obtained on different days. optimal conditions for freezing of both effector and target cells were quite similar. dimethyl sulfoxide (dmso) at a concentration of 7.5-10.0% was employed as the cryoprotective agent and cells were frozen at the rate of -1 degrees c/minute. the handling procedures for the cells before and after fre ... | 1977 | 839557 |
| a heteroduplex study of the sequence relationships between the rnas of m-msv and m-mlv. | the regions of sequence homology between the rna genomes of a murine sarcoma virus (clone 124 moloney-msv) and its parental helper virus (clone 3 moloney-murine leukemia virus (m-mlv)) have been mapped. long complementary dna transcripts of the m-mlv rna were hybridized to m-msv rna, and the structures of the hybrids were observed in the electron microscope. beginning at the 5' end, the two rnas are homologous for a region of length 2.25 kb (kilobases). in the next region, of length approximatel ... | 1977 | 844103 |
| augmentation of natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic target cells. | the levels of natural cell-mediated cytotoxicity against tumor cells in young balb/c and balb/c nude mice could be augmented by inoculation of a variety of mouse tumor cells or of mouse thymocytes. in older mice with low levels of spontaneous cytotoxic reactivity, inoculation of tumor cells led to rapid appearance of cytotoxicity. this augmented cytotoxicity reached a peak 3 days after inoculation and then declined rapidly. the specificity of the augmented cytotoxicity appeared to be the same as ... | 1977 | 844921 |
| defective friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. | defective friend spleen focus-forming virus (sffv) is able to interfere with the ability of its naturally occurring leukemia-inducing helper virus (llv-f) to induce xc plaque formation in several different strains of mouse embryo cells. this interference has been observed by using two different sffv preparations, one contained in an nb-tropic stock of friend virus (fv) complex, and the second present in a c57bl-adapted strain of fv complex containing an associated b-tropic llv-f helper. the llv- ... | 1977 | 904028 |
| phenotypic markers in human skin fibroblasts as possible diagnostic indices of hereditary adenomatosis of the colon and rectum. | hereditary adenomatosis of the colon and rectum (acr) and its gardner's syndrome variant, an autosomal dominant trait, indicate a propensity for neoplasia. the present study describes the growth abnormalities of cultured human skin fibroblasts derived from normal-appearing cutaneous biopsies of acr genotypes and a portion of the clinically asymptomatic acr progeny, first filial generation, and their differential susceptibility to transformation by kirsten murine sarcoma virus. these skin fibrobl ... | 1977 | 922693 |
| murine sarcoma viruses: the helper-independence reported for a moloney variant is unconfirmed; distinct strains differ in the size of their rnas. | 1977 | 189494 | |
| effect of pretreatment with immune serum on murine sarcoma virus (moloney) tumour induction and growth. | regressor serum from msv-m-infected mice markedly reduced msv-m oncogenesis when administered i.p. (0-1 ml/mouse) as much as 30 days before i.m. msv-m infection in adult balb/c mice. the regressor serum activity appeared to be directly dependent on the amount of igg, as shown by: (1) inactivity of sera which have low virus-neutralizing antibody content; (2) high effectiveness only of the igg serum fraction; (3) inactivity of regressor serum incubated with anti-mouse gamma-globulin serum. the reg ... | 1977 | 189792 |
| differential genetic susceptibility of cultured human skin fibroblasts to transformation by kirsten murine sarcoma virus. | hereditary adenomatosis of the colon and rectum (acr), an autosomal dominant trait, is associated with a predisposition to neoplasia. the present study describes the differential genetic susceptibility of cultured human skin fibroblasts to transformation by kirsten murine sarcoma virus. primary cutaneous outgrowths were derived from normal appearing subepidermal biopsies of acr phenotypes and appropriate controls. exponentially growing cell cultures from acr subjects and a portion of the clinica ... | 1977 | 189938 |
| expression of viral envelope glycoprotein and transformation genes in cells transformed by a defective kirsten murine sarcoma virus. | 1977 | 190782 | |
| loss of fv-1 restriction in balb/3t3 cells following infection with a single n tropic murine leukemia virus particle. | the ability of various murine leukemia viruses (mulvs) to replicate in mouse cells exhibiting fv-1 restriction was analyzed by quantitative dose-response assays. in particular, the effect of infection with n, b, or nb tropic mulvs on fv-1b restriction in balb/3t3 cells was measured with an infection center technique in which pseudotypes of murine sarcoma virus (msv), which have been shown to exhibit fv-1 dependence of expression, were used to quantitate the degree of restriction. the resulting d ... | 1977 | 191197 |
| stimulation of mouse migration inhibitory factor (mif) production form msv-immune lymphocytes by soluble tumor-associated antigen: requirement for histocompatible macrophages. | spleen cells from c57bl/6 mice immunized with murine sarcoma virus (msv) are capable of producing migration inhibition factor (mif) in response to stimulation with a specific tumor-associated antigen prepared by solubilization with 3 m kcl. we have previously demonstrated that this response is t cell-dependent. further investigations into the effector cells involved in the production of mif have revealed that spleen cells from mice immunized with msv cannot produce mif when stimulated with tumor ... | 1977 | 191532 |
| enhancement of tumor induction in rats with moloney murine sarcoma virus by a "new" method based on direct injection into fetuses. | undiluted, fivefold-diluted, and 25-fold-diluted doses of a stock of moloney murine sarcoma virus were injected directly, in a volume of 0.025 ml, into the backs of fetal sprague-dawley rats by laparotomy through the uterine wall at 18 days of gestation. during the first 8 weeks after birth the young responded to the virus with remarkably high but dose-dependent incidences of neoplasms. when a one-fifth dilution of the virus preparation was inoculated at fetal ages 16, 18, and 20 days, the incid ... | 1977 | 264591 |
| intratibial moloney sarcoma virus-induced osteosarcoma in the rat: tumor incidence and pathologic evaluation. | osteosarcomas were induced in approximately 80% of young new zealand black rats by the intratibial inoculation of moloney murine sarcoma virus from day 1 to day 5 after birth. the neoplasms were composed of a spectrum of well-differentiated to poorly differentiated osteoblasts, osteocytes, and osteoclasts. budding of c-type viral particles was associated with tumor induction. compared to rats inoculated on day 1 after birth, rats inoculated at 4 days of age developed consistently more osteoproli ... | 1977 | 264594 |
| cleavage map of linear mouse sarcoma virus dna. | proviral dna transcribed from the rna of moloney murine sarcoma virus was isolated from newly infected cells. three forms of viral dna were observed: (i) a linear double-stranded form of 3.4 x 10(6) daltons which constituted the major viral dna species in the cell, and is thought to be a complete transcript (monomer) of viral rna; (ii) a fast-sedimenting viral dna bigger than the monomeric unit which can be either integrated provirls or concatamers; and (iii) covalently closed circles of monomer ... | 1977 | 264682 |
| characterization of human cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine. | human osteosarcoma (hos) clonal cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) were characterized, and compared to non-producer hos cells transformed by kirsten murine sarcoma virus (ki-msv). the mnng- and virus-transformed cells grew in the aggregate form above an agar base, grew in soft agar, and had a high fibrinolytic activity. when inoculated into nude mice, all the chemically or virally altered cells produced tumors or tumor nodules. when transplanted into ats-tr ... | 1977 | 265298 |
| pathology of neoplasms and other lesions induced in rats with moloney murine sarcoma virus. | moloney murine sarcoma virus (m-msv) was injected directly into the fetuses of sprague-dawley rats during the late stage of gestation and into the neonates within 24 hours after birth. ninety rats developed 188 neoplastic lesions during the 8-week period of observation. nearly all of the neoplasms were of mesenchymal derivation. sixty percent of these neoplasms revealed more complex histologic features than those previously reported for neoplasms induced in rodents with m-msv and were designated ... | 1977 | 266356 |
| murine sarcoma virus (msv)-induced tumors in mice. i. distribution of msv-immune cytolytic t lymphocytes in vivo. | quantitative studies using a 51cr release assay were performed to analyse the time-course of appearance and specificity of cytolytic cells in c57bl/6 mice inoculated with moloney murine sarcoma virus (msv). various lymphoid organs were studied including spleen, mesenteric lymph nodes (mln), lymph nodes regional to the msv-induced tumor (rln), and peripheral blood. furthermore, the kinetics of appearance of cytolytic cells within the msv-induced tumor was determined. in agreement with previous st ... | 1977 | 300075 |
| t and b lymphocyte migration into syngeneic tumors. | the migration of splenic t and b lymphocytes into syngeneic tumors undergoing immunologic rejection was investigates. spleen cells were obtained from normal balc/c mice or balb/c mice bearing tumors induced by murine sarcoma virus (msv). either whole spleen cells or immunoabsorbent purified t and b cells were radiolabeled with sodium chromate-51 and injected i.v. into normal or msv inducted-tumor bearing syngeneic recipients. twenty-four hours later the recipient mice were sacrificed and radioac ... | 1977 | 300384 |
| epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (egf) receptors and the effect of mouse sarcoma virus transformation. | fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, nrk. these clones were studied for their ability to bind epidermal growth factor (egf), susceptibility to transformation by mouse sarcoma virus (msv), and alteration in egf binding upon sarcoma virus transformation. the epithelioid clones bound much more egf than the fibroblastic clones; scatchard plots on two of these clones, one epithelioid and one fibroblastic, showed that the higher egf binding (1.3 x 10( ... | 1978 | 304450 |
| monovalent antibodies directed to transformation-sensitive membrane components inhibit the process of viral transformation. | monovalent antibodies (fab) directed to two classes of transformation-sensitive cell surface components, ganglioside and galactoprotein a (gap a), inhibit the process of oncogenic viral transformation of cells. mouse 3t3 cells infected with murine sarcoma virus were not transformed in terms of morphology change and enhancement of sugar uptake when the infected cells were cultured in the presence of monovalent antibodies directed to gm(1) ganglioside or to gap a. transformation inhibitory activit ... | 1978 | 216010 |
| chemical transformation of human revertant cells induced by murine sarcoma virus. | a human revertant cell line, derived from non-producer human osteosarcoma cells (np/khos) induced by kirsten murine sarcoma virus, was treated in vitro with various levels of polycyclic aromatic hydrocarbons (3-methylcholanthrene, 7,12-dimethylbenz(alpha)anthracene, and benzo(alpha)pyrene [bp] or dimethyl sulfoxide (control). cells treated only with 3-methylcholanthrene and 7,12-dimethylbenz(alpha)anthracene underwent morphological alteration in vitro, and produced tumors when injected into nih ... | 1978 | 415010 |
| activity of 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride against experimental tumors in mice and rats. | 10-chloro-5-(2-dimethylaminoethyl)-7h-indolo [2,3-c]-quinolin-6(5h)-one hydrochloride (ciq) was shown to exert significant antitumor activity against the ehrlich carcinoma and sarcoma 180 transplantable tumors in mice by the intraperitoneal (ip) or oral (po) routes and when incorporated into diet. a solid tumor induced in balb/c mice by the subcutaneous (sc) implantation of nonproducer murine sarcoma virus-transformed balb/3t3 cells was also inhibited by ciq after ip or po treatment but there wa ... | 1978 | 579626 |
| anti-viral activity induced by culturing lymphocytes with tumor-derived or virus-transformed cells. identification of the anti-viral activity as interferon and characterization of the human effector lymphocyte subpopulation. | a viral inhibitor(s) is released in the supernate of mixed cultures containing human or mouse lymphocytes and cells from certain lines. the inhibitor is active against a variety of unrelated viruses and is a protein that is not toxic for cells. it does not inactivate viruses directly, but inhibits viral replication through an intracellular mechanism that involves synthesis by the cells of both rna and protein. these characteristics identify the inhibitor as an interferon. the anti-viral activity ... | 1978 | 650155 |
| in situ fc receptor-bearing cells in two murine tumors. i. isolation and identification. | the in situ inflammatory response was studied in 2 different tumors, the t1699 mammary adenocarcinoma in inbred dba/2 mice and a sarcoma induced in inbred c57bl/6 mice by murine sarcoma virus. the presence of fc receptors (fcr's) on infiltrating host cells was determined by rosette formation with antibody-coated sheep red blood cells (ea). differences in fcr-binding activity among various cells were detected with the use of ea prepared with a range of antibody concentrations. cells bearing fcr r ... | 1978 | 650702 |
| effects of hydroxyurea on murine type c virus-specific dna synthesis in newly infected cells. | cell transformation and replication of the rauscher pseudotype of moloney murine sarcoma virus in mouse embryo fibroblasts were inhibited by hydroxyurea within a critical time period of 30 to 90 min postinfection. in cells infected by rauscher leukemia virus, treatment with 1mm hydroxyurea during the critical time period resulted in the accumulation of minus-strand dna (molecular weight, 3 x 10(6)) in association with the parental viral genoma rna. this 5 to 6 x 10(6) dalton rna:dna hybrid was f ... | 1978 | 657143 |
| protective effect of murine sarcoma virus-superinfected mouse tumor cells against outgrowth of corresponding noninfected tumor. | cultured lines of three newly established methylcholanthrene-induced tumors, mbk and mbl in cba and mc57m in c57bl mice, and two mammary tumors, sbfnhc in cba and s3w in asw mice, were superinfected in vitro with moloney sarcoma or leukemia virus (msv, mlv). after superinfection, they expressed the moloney virus-determined cell surface antigen (mcsa) and murine c-type viral p30 antigen, and produced nb-tropic c-type virus. the virus-infected tumors became more rejectable in normally susceptible ... | 1978 | 705875 |
| comparative immunological studies on cryosurgery and surgical operation using moloney murine sarcoma virus-induced primary tumors in balb/c mice. | the effect of cryosurgery and surgical operation on moloney murine sarcoma virus-induced primary tumors in the treated and untreated groups of balb/c mice was compared in terms of tumor growth, cumulative mortality, lymphocyte-mediated cytotoxicity, proliferative response of lymphocytes, and humoral antibody formation. the results indicate that cryosurgical treatment showed both in vivo and in vitro effects; (i) tumor growth and cumulative mortality in mice treated by cryosurgery were significan ... | 1978 | 710802 |
| revertants of mouse cells transformed by murine sarcoma virus. v. loss of msv-specific nucleotide sequences from cellular rna. | 1978 | 217157 | |
| decreased alkaline phosphatase in cells transformed by rous sarcoma virus. | chick embryo cells transformed by either of two strains of rous sarcoma virus (bryan high titer or schmidt-ruppin) have low levels of alkaline phosphatase activity compared with nontransformed chick embryo cells. essentially no differences in acid phosphatase activity were observed between these transformed and nontransformed cells. a virus mutant, rsv-bh-ta, induces temperature-dependent transformation in infected cells. at 41 degrees, the transformation-nonpermissive temperature, alkaline phos ... | 1978 | 202384 |
| identification of a 30s rna with properties of a defective type c virus in murine cells. | a novel species of 30s rna has been detected in a variety of mouse cell lines. the 30s rna is specifically packaged by helper-independent type c viruses propagated in such cells. nucleic acid hybridization detects no homology between the 30srna and the genomic rna of helper-independent mouse type c viruses. the properties of the 30s rna suggest that it is a defective endogenous mouse type c virus and that it is analogous to a previously described class of defective endogenous rat type c virus, w ... | 1978 | 202730 |
| glucocorticoids induce focus formation and increase sarcoma viral expression in a mink cell line that contains a murine sarcoma viral genome. | dexamethasone (3 x 10(-10) to 3 x 10(-6) m) induced foci of morphologically transformed cells in a small proportion of a mink cell line that contains the moloney murine sarcoma viral genome (s+l-). the induction was glucocorticoid specific, since other steroids with glucocorticoid activity (prednisolone, cortisol, and aldosterone) induced foci with an efficiency that paralleled their glucocorticoid activity, and steroids lacking glucocorticoid activity (17b-estradiol, testosterone, and progester ... | 1978 | 202733 |
| immunological study of two stocks of moloney sarcoma virus producing regressor and progressor tumors in c57bl/6 mice. | the immune response to progressor murine sarcoma virus (p-musv)2 and regressor murine sarcoma virus (r-musv) was analyzed by the radioisotopic footpad assay (fpa) for delayed hypersensitivity (dh) and by the membrane immunofluorescence assay for anti-tumor antibodies. in mice injected with p-musv, the footpad response remained at a low level for 7 weeks, while membrane immunofluorescence antibody titers reached their maximum levels after 5 weeks. by contrast, in mice injected with r-musv, the fo ... | 1978 | 203543 |
| in situ embedding method of cells grown in beem capsules for immunoelectron microscopic studies of oncornaviruses. | a technique of in situ embedding of cells grown in beem capsules has been devised for immunoelectron microscopic studies of oncornaviruses. as compared to other immunoelectron microscopic procedures, this technique is less time and reagent-consuming. the quality and specificity of this method were tested on well-characterized mouse mammary tumor virus (type b virus) and murine sarcoma virus (type c virus particles). this method gave good results in labeling of the virus particles with ferritin o ... | 1978 | 203626 |
| effect of the fv-1 locus in vivo: host range pseudotypes of murine sarcoma virus. | fv-1-specific host-range pseudotypes of murine sarcoma virus (musv) were developed by rescue from nonproducer cells with n- or b-tropic leukemia viruses. the musv(b) and musv(n) pseudotype viruses were tested in vitro and were restricted specifically by the fv-1 gene locus. when the pseudo-type viruses were tested in vivo in mice of specific fv-1 geno-types, tumor induction was completely restricted in nonpermissive animals, including athymic nude mice, whereas tumors grew progressively in permi ... | 1978 | 204797 |
| electron microscopic studies of intracisternal virus particles in soehner-dmochowski murine sarcoma virus-induced bone tumors of new zealand black rats. | soehner-dmochowski murine sarcoma virus (moloney)-induced bone tumors of new zealand black rats carry two morphologically different types of virus particles, namely, extracellular type c and intracisternal virus particles, which have thus far not been reported. these two types of virus particles have also been observed in the tissue culture cells derived from normal prostate tissues of a/dm and balb/c/dm mice after inoculation of cell-free extracts of these bone tumors. the intracisternal virus ... | 1978 | 205348 |
| studies with the baboon endogenous virus and its pseudotype of murine sarcoma virus in marmosets. | three marmoset species (saguinus oedipus oedipus, s. fuscicollis, callithrix jacchus) failed to show evidence of infection or disease following inoculation of baboon endogenous type-c virus (baev). cells infected with a kirsten murine sarcoma-baev pseudotype (msv[baev]) produced a progressive fibrosarcoma in s. o. oedipus and tumors in s. fuscicollis and c. jacchus which regressed. tumor formation is believed to be due to growth of inoculated, virus-infected cells and not transformation of marmo ... | 1978 | 205851 |
| fbj osteosarcoma virus in tissue culture. iii. isolation and characterization of non-virus-producing fbj-transformed cells. | hamster and rat cell lines have been established that have been transformed by fbj murine sarcoma virus (fbj-musv) but that do not produce virus. the hamster cell line originated from an osteosarcoma that appeared in a hamster inoculated at birth with an extract of a cfno1 mouse fbj-osteosarcoma. the rat cell line was obtained by transferring the fbj-musv genome to normal rat kidney cells in the absence of the fbj type c virus (fbj-mulv), which, usually in high concentration, accompanies the fbj ... | 1978 | 206718 |
| effects of culture conditions on the growth and differentiation of transformed rat adrenocortical cells. | normal rat adrenocortical cells grow in vitro in two states of differentiation, depending on culture conditions. under one set of conditions, they proliferate and are fibroblast shaped, with some myoid cell characteristics and with limited steroidogenic activity. under alternate conditions they do not replicate and are epithelial-like and similar to adrenocortical secretory cells structurally and steroidogenically. the effects of oncogenic transformation on these cellular responses to culture va ... | 1978 | 207413 |
| the "sarcoma-specific" region of moloney murine sarcoma virus 124. | labeled, purified 30s rna from moloney murine sarcoma virus was annealed to an excess of moloney murine leukemia virus complementary dna. upon treatment of the resulting dna.rna hybrids with rnase h followed by sucrose gradient sedimentation, and undigested 18s rna molecule was recovered. this rna molecule was shown to represent the "sarcoma-specific" region of the virus. the unintegrated linear dna provirus of murine sarcoma virus 124 was isolated from newly infected cells and a physical map of ... | 1978 | 208071 |
| in vitro isolation of stable rat sarcoma viruses. | a sprague-dawley (sd-1) rat embryo culture, at low passage level, released an endogenous ecotropic type c virus (sd-ralv) and after about 20 further passages it underwent spontaneous transformation. the sd-ralv, released from the transformed cells, did not cause rapid transformation of other rat embryo cells. however, when the transformed cells were repeatedly cocultivated with three different chemically transformed and serially transplanted rat tumor cell lines (sarcoma, carcinoma, and hepatoma ... | 1978 | 208081 |
| transformation of indian muntjac cells by murine and avian sarcoma viruses. | indian muntjac cells were efficiently transformed by murine sarcoma virus (msv) and avian sarcoma viruses (asv). when colony formation of the infected cells was examined in soft agar, many colonies were formed by the asv-injected cells but no colony was seen in the msv-infected cells. the asv-transformed cell clones differed among the clones in morphology, presence of inducible asv genome, and karyotypes. | 1978 | 208907 |
| two different murine sarcoma virus isolates have homologous genome sequences: implication for their origin. | 1978 | 210574 | |
| comparison of the genomic organization of kirsten and harvey sarcoma viruses. | current studies were undertaken to compare the genomes of kirsten murine sarcoma virus (ki-musv), harvey murine sarcoma virus (ha-musv), and the replication-defective endogenous rat virus to understand the function of these viral rnas. genome organization and sequence homology were studied by fingerprinting large rnase t1-resistant oligonucleotides and by cross-protecting homologous oligonucleotides against rnase a and t1 digestion with complementary dna prepared from each of the other viral rna ... | 1978 | 211254 |
| growth factors from murine sarcoma virus-transformed cells. | murine sarcoma virus-transformed mouse fibroblasts produce polypeptide growth factors and release them into serum-free medium. these factors stimulate cells to divide in monolayer cultures and also to form colonies that grow progressively soft agar. three major peaks of activity are seen, with apparent molecular weights of 25,000, 12,000, and 7000. the sarcoma growth factors are heat-stable, trypsin-sensitive, and active in nanogram quantities when tested for growth stimulation of untransformed ... | 1978 | 211512 |
| the effect of mouse interferon on the transformation of nih/3t3 mouse cells by murine sarcoma virus. | 1978 | 211721 | |
| nucleases and adenosine 3',5'-cyclic monophosphate phosphodiesterase activities in murine sarcoma virus (moloney)-infected mice. | to provide information on the role of nucleases in oncogenic virus infection, the activities of 3'-nucleotide phosphodiesterase (3'-npdase), 5'-nucleotide phosphodiesterase (5'-npdase), acid deoxyribonuclease (dnase ii), and 3',5'-cyclic amp phosphodiesterase (campdase) in spleen extracts of murine sarcoma virus-infected c57bl/6 inbred mice were studied. at the peak of tumor growth and of the cell-mediated cytotoxic response (cmc) against tumor-associated antigens, 3'-npdase, 5'-npdase, and dnas ... | 1978 | 212566 |
| viral gene expression in murine sarcoma virus(murine leukemia virus)-infected cells. | nih 3t3 cells infected with moloney murine sarcoma virus (murine leukemia virus) produce virions which contain about 99% murine sarcoma virus rna and 1% murine leukemia virus rna. this report describes experiments which measured intracellular concentrations of proviral dna and rna transcripts for each of the viruses. we found that three to four copies of proviral dna from each virus were integrated into the cellular dna. measurements of rna specific for each of the genomes by hybridization to sp ... | 1978 | 212592 |
| phosphorylation of chromatin- and ribosome-associated proteins in cells transformed by adenovirus, murine sarcoma virus, and methylcholanthrene. | endogenous protein phosphorylation in chromatin and ribosomes of monkey, mouse, and rat cells transformed by dna, rna tumor viruses, and a chemical carcinogen revealed the association of a protein of approximately 90,000 daltons (90k), which is highly phosphorylated in vitro. peptide map analysis showed that the 90k proteins associated with these organelles of various transformed cells are similar irrespective of the species of cells and transforming agents. this species of protein could not be ... | 1978 | 212594 |
| structural markers on core protein p30 of murine leukemia virus: functional correlation with fv-1 tropism. | tryptic peptide maps from more than 50 isolates of murine leukemia virus (mulv) have shown that, in general, the structure of core protein p30 is highly conserved. however, a structurally variable region of p30 has been identified that is functionally associated with fv-1 tropism. on the basis of this structural variability, mulv strains can be classified as b-tropic, n-tropic, xenotropic, and/or as being derived from wild mice. certain xenotropic viruses have a p30 like that of b-tropic mulv an ... | 1978 | 212738 |
| stimulatory effect of immunization on tumor induction by moloney murine sarcoma virus. | adult mice were immunized with varying doses of inactivated moloney murine leukemia virus (m-mulv). eight weeks after immunization, mice were challenged with a dose of moloney murine sarcoma virus (m-musv) that could induce tumors in approximately 50% of normal animals. mice immunized with high doses of m-mulv (10(10) particles) had significantly decreased tumor incidences, whereas mice immunized with low doses of m-mulv (10(2) particles) had significnatly increased tumor incidences compared to ... | 1978 | 213613 |
| ornithine decarboxylase activity in cells acutely and chronically transformed by murine sarcoma virus. | 1978 | 213779 | |
| [effect of sodium butyrate on the interferon sensitivity of normal or cells transformed by murine sarcoma virus]. | sodium butyrate enhances the sensitivity of msv transformed murine cells to interferon, but does not influence the response of normal cells. sodium butyrate might act in transformed cells by restoring the different elements of the cytoskeletal system. | 1978 | 214252 |
| a replication-defective variant of moloney murine leukemia virus. i. biological characterization. | we have studied the virus produced by a clone, termed 8a, that was isolated from a culture of murine sarcoma virus-transformed mouse cells after superinfection with moloney murine leukemia virus (mulv-m). clone 8a produced high levels of type c virus particles, but only a low titer of infectious murine sarcoma virus and almost no infectious mulv. when fresh cultures of mouse cells were infected with undiluted clone 8a culture fluids, they released no detectable pogeny virus for several weeks aft ... | 1978 | 74421 |
| further evidence for deletion of envelope glycoprotein (gp69/71) sequences in formation of moloney-murine sarcoma virus. | moloney-murine sarcoma virus (s+l- strain of m-msv) has been nonproductively cloned in murine and non-murine host cells (s+l- cells) and the expression of moloney leukaemia virus (m-mulv) 30000 mol. wt. core protein (p30) and envelope glycoprotein (gp69/71) were studied by radioimmunoassay. antigenic determinants of the m-mulv p30 were associated with the sarcoma virus genome in these non-productively transformed cell clones studied, while the determinants of m-mulv gp69/71 were not. the absence ... | 1978 | 80447 |
| secondary in vitro generation of cytolytic t-lymphocytes (ctl's) in the murine sarcoma virus system. virus-specific ctl induction across the h-2 barrier. | following in vitro stimulation of murine sarcoma virus moloney isolate (m-musv)-immune spleen cells with syngeneic antigenically related moloney leukemia cells, highly efficient cytotoxic t-lymphocytes (ctl's) were generated. the cytotoxic effect was directed only against h-2-compatible target cells bearing m-musv tumor-associated antigens (taa). however, in a cold target competition assay a weak but detectable capacity to block ctl activity was also obtained when allogeneic moloney leukemia cel ... | 1978 | 80457 |