Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the matrix protein. | the sequence of the matrix (m) protein gene and contiguous intergenic regions of the human parainfluenza 3 virus (pf3) was determined by molecular cloning. the encoded m protein contains 354 amino acids and has a predicted mol wt of 39,506. the m protein amino acid sequence was compared to the homologous proteins from other members of the paramyxoviridae family. the pf3 protein shared 61% homology with the sendai virus protein and approximately 35% homology with measles and canine distemper viru ... | 1987 | 3029963 |
| parainfluenza type 3 in newborns. | 1987 | 3031576 | |
| nucleotide sequence of the bovine parainfluenza 3 virus genome: its 3' end and the genes of np, p, c and m proteins. | we present the nucleotide sequence of bovine parainfluenza 3 virus (bpiv3) genome from its 3' end to the opening region of the f gene, through the np, p plus c, and m genes. comparison of the sequence with those reported for other paramyxoviruses indicated that bpiv3 was most similar to human parainfluenza 3 virus (hpiv3), and also very similar to sendai virus in the structural make-up of its genome and the amino acid sequences of its gene products, suggesting that these three viruses constitute ... | 1987 | 3031614 |
| nucleotide sequence of the bovine parainfluenza 3 virus genome: the genes of the f and hn glycoproteins. | by analysing complementary dna clones constructed from genomic rna of bovine parainfluenza 3 virus (bpiv3), we determined the nucleotide sequence of the region containing the entire f and hn genes. their deduced amino acid sequences showed about 80% homologies with those of human parainfluenza 3 virus (hpiv3), about 45% with those of sendai virus, and about 20% with those of sv5 and newcastle disease virus (ndv), indicating, together with the results described in the preceding paper on the np, p ... | 1987 | 3031615 |
| nucleotide sequence of the human parainfluenza virus 3 matrix protein gene. | 1987 | 3031622 | |
| alveolar macrophage phagocytic kinetics following pulmonary parainfluenza-3 virus infection. | qualitative and quantitative evaluations of the cellular components of bronchoalveolar washings of calves with experimental parainfluenza-3 virus pneumonitis and control calves were made. calves were exposed to 10(9) tcid50 of pi-3 by intranasal aerosol exposure and bronchoalveolar cells obtained 7 days after infection by volume-controlled bronchopulmonary lavage. transient tachypnea and pyrexia occurred in all infected calves, and virus was recoverable at 7 days from nasal swabs and lung tissue ... | 1987 | 3033112 |
| nucleotide sequence of the coding and flanking regions of the human parainfluenza virus type 3 fusion glycoprotein gene. | the complete nucleotide sequence of the human parainfluenza virus type 3 (hpiv3) fusion (f) protein gene has been determined. the hpiv3 f gene is 1851 nucleotides long including six u residues in the genomic rna, which probably direct synthesis of the first few nucleotides in the f mrna polyadenylate tail. the hpiv3 f gene contains a single long open reading frame coding for 539 amino acids. the predicted molecular weight of the unglycosylated precursor f0 protein was 60031. four potential carbo ... | 1987 | 3033123 |
| a case of rhabdomyolysis in chronic renal failure. | rhabdomyolysis can be induced by a variety of physical and chemical insults to skeletal muscle. though there are only a few reports of myositis associated with viral diseases, and no reported cases of virus-induced rhabdomyolysis in uremic patients. chronic uremic states have been known to potentiate a variety of metabolic and immunological abnormalities. present case had an acute, progressive and fatal rhabdomyolysis which was thought to be induced by virus infection. a 43-year-old man had rece ... | 1987 | 3033362 |
| giant cell pneumonia caused by parainfluenza type 3 in a patient with acute myelomonocytic leukemia. | a 4 1/2-year-old boy with acute myelomonocytic leukemia developed fever, neutropenia, and a prolonged respiratory illness while receiving maintenance chemotherapy. an open lung biopsy specimen demonstrated a giant cell pneumonia with intracytoplasmic and probable intranuclear viral inclusions of the paramyxovirus type. serologic studies demonstrated convincing evidence of a parainfluenza type 3 infection. although parainfluenza type 3-induced giant cell pneumonia has been reported in infants wit ... | 1987 | 3034189 |
| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| [effect of tuberculin and levamisole on the immune response after vaccinating calves against para-influenza and salmonella infections]. | studied was the effect of tuberculin and levamizole on the immune response following vaccination of calves against parainfluenza and salmonella infections. it was found that in calves that had colostrum antibodies against parainfluenza the immunogenic stimulation with the two agents first led to a drop of the total protein and of the antibody titer of the blood serum; in later periods the antibody titer grew severalfold. the stimulation with tuberculin and levamizole in the case of salmonella in ... | 1987 | 2820117 |
| structural proteins of bovine parainfluenza-3 virus. | six structural proteins of bovine parainfluenza-3 virus (pi-3v) labeled with [35s]-methionine could be resolved by polyacrylamide gel electrophoresis (page). five structural proteins of this virus had been previously reported. the 6 proteins found in this study were: l, a 180,000 (180 kd) molecular weight (mw) large protein; p, 83 kd phosphoprotein; hn, 69 kd hemagglutinin-neuraminidase glycoprotein; np, 66 kd nucleocapsid protein; f, 55 kd fusion glycoprotein; and m, 38 kd matrix protein. selec ... | 1987 | 2821671 |
| expression of biologically active and antigenically authentic parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein by a recombinant baculovirus. | the hemagglutinin-neuraminidase (hn) gene of human type 3 parainfluenza virus has been inserted into a baculovirus expression vector under the control of the polyhedrin promoter. hn protein produced in insect cells by the recombinant baculovirus appeared to be glycosylated, was transported to the cell surface, and was biologically active. all of the hn epitopes previously mapped functionally to a region(s) involved in neuraminidase and/or hemagglutination activities were conformationally unalter ... | 1987 | 2821684 |
| nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses. | the nucleotide sequence of the human parainfluenza virus 3 (hpiv3) hemagglutinin-neuraminidase (hn) gene has been determined using cdna clones derived from both hpiv3 genomic rna and mrna. the hn mrna contains 1,882 nucleotides, not including the poly(a) tail. primer extension experiments were carried out to locate the 5' terminal nucleotide of the hn mrna. the 3' end of the mrna was located at a putative polyadenylation signal. the hpiv3 hn mrna has one large open reading frame that codes for 5 ... | 1987 | 2822598 |
| respiratory viruses in children admitted to hospital in edinburgh 1972-1985. | the temporal incidence of respiratory viruses identified in children admitted to an edinburgh hospital over a period of 14 years, 1972-1985, is described, respiratory syncytial (rs) virus was identified most often, usually (but not in 1979 and 1985) with single peaks of activity in the winter months. influenza virus infections were seen in the winter and spring but we failed to find evidence of interference between rs and influenza virus. of the viruses studied, the parainfluenza viruses were th ... | 1987 | 2822808 |
| expression of the f and hn glycoproteins of human parainfluenza virus type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity. | cdna clones containing the complete coding sequences for the human parainfluenza virus type 3 (piv3) fusion (f) and hemagglutinin-neuraminidase (hn) glycoprotein genes were inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of the p7.5 early-late vaccinia virus promotor. the recombinant vaccinia viruses, designated vaccinia-f and vaccinia-hn, expressed glycoproteins in cell culture that appeared to be authentic with respect to glycosylation, disulfide linkage ... | 1987 | 2822951 |
| [use of an immune blotting method (western) for studying viral antigens and for detecting antibodies to viral proteins]. | the technique of the procedure and the results of the use of immune ("western") blotting method for studies on viral antigens and detection of antibodies to individual proteins of viral particles are described. the possibility of detection and study of individual viral antigens in the whole plasma of patients or carriers is demonstrated by the example of hbs antigen of human hepatitis b virus. the method of immune blotting was used for screening of human sera for the detection of antibodies to t ... | 1987 | 2823482 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, f and hn. | the sequence of the genes encoding the fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins of the human parainfluenza 3 virus was determined by molecular cloning. the genes were cloned by primer extension using genomic 50 s rna as the template. a series of four overlapping clones was generated from the 3' end of the fusion gene which extended across the gene end and intergenic boundaries of the f-hn and hn-l genes. the f gene extends 1851 nucleotides (inclusive of the putative transcri ... | 1987 | 2825443 |
| prevalence of antibodies to some viral pathogens, brucella abortus and toxoplasma gondii in serum from camels (camelus dromedarius) in sudan. | 1987 | 2825450 | |
| [progress of the campaign against acute respiratory viral diseases in children]. | 1987 | 2826029 | |
| [infectious respiratory tract diseases in cattle in the winter of 1985/86: detection of infection by bovine respiratory syncytial virus and parainfluenza virus type 3 by immunofluorescence in nasal swab samples and by antibody titer increase in serum (elisa)]. | 1987 | 2827303 | |
| protection against respiratory disease in calves induced by vaccines containing respiratory syncytial virus, parainfluenza type 3 virus, mycoplasma bovis and m dispar. | a field trial to assess the ability of two vaccines to protect calves against respiratory disease was carried out on a large beef rearing unit in southern england over the two winters of 1983 to 1984 and 1984 to 1985. a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma bovis and m dispar or a vaccine containing only the respiratory syncytial virus component were inoculated into 246 and 245 calves, respectively; 245 calves r ... | 1987 | 2827366 |
| [experiences with the riems vaccine combination pi-3 and riems ibr/ipv vaccines in cattle-raising stock]. | 1987 | 2831841 | |
| [immune response of calves following vaccination with paramyxovirus parainfluenzae-3 and adenovirus-1 under different colostral immunity stresses]. | studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against p. parainfluenzae-3 and adenovirus-1. for the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) cpe50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. for calves which missed these antibodies, t ... | 1987 | 2833005 |
| antigenic and structural properties of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3: sequence analysis of variants selected with monoclonal antibodies which inhibit infectivity, hemagglutination, and neuraminidase activities. | the hemagglutinin-neuraminidase (hn) gene sequence was determined for 16 antigenic variants of human parainfluenza virus type 3 (piv3). the variants were selected by using monoclonal antibodies (mabs) to the hn protein which inhibit neuraminidase, hemagglutination, or both activities. each variant had a single-point mutation in the hn gene, coding for a single amino acid substitution in the hn protein. operational and topographic maps of the hn protein correlated well with the relative positions ... | 1987 | 2437318 |
| immediate persistent infection by human parainfluenza virus 3: unique fusion properties of the persistently infected cells. | we describe here a persistently infected cell system with unique properties. cells infected with human parainfluenza virus type 3 (pf3) at high multiplicities of infection showed little or no cytopathic effects (cell fusion). unlike other paramyxovirus persistent infections that require a long development time, the majority of the cells survived the initial infection and formed persistently infected cell cultures that were immediately available for study. in addition, unlike other paramyxovirus ... | 1987 | 2438380 |
| enhancement by parainfluenza 3 infection of contractile responses to substance p and capsaicin in airway smooth muscle from the guinea pig. | guinea pigs were inoculated by nasal insufflation with parainfluenza 3 (p-3) or virus growth medium 4 days before performing in vitro pharmacologic studies on left bronchial ring segments. cumulative dose-response studies with capsaicin revealed an enhanced contractile response after p-3 infection. the sensitivity and magnitude of contractile effects of substance p in the left bronchi were also enhanced by p-3 infection. after pretreatment of the isolated tissues with phenoxybenzamine to block h ... | 1987 | 2443048 |
| [study of the multiplication in cell cultures of two strains of para-influenza virus type 3. iii. multiplication in bhk 21 cells]. | study was conducted on the multiplication of two strains (c243 and d) of parainfluenza virus type 3 in bhk 21 cells. multiplication curve of the virus was established and immunohistochemical aspects of the process were investigated. chronological study of successive steps of the formation and development of viral components allowed to see that the virus multiplication rate is low in this cell system. the parainfluenza antigen became detectable by immunofluorescence in the infected cell perinucle ... | 1987 | 2444030 |
| parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies. | thirteen monoclonal antibodies (mabs) were prepared against human parainfluenza virus type 2 (piv2). these mabs reacted with the haemagglutinin-neuraminidase glycoprotein with an mr of 84k. the mabs defined one antigenic site which could be divided into five epitopes. a correlation between haemagglutination inhibition (hi) and neutralization activity could be seen although one mab, which recognized a distinct epitope, showed neutralization and no hi activity to piv2. the reactivity of the mabs w ... | 1988 | 2451708 |
| parainfluenza 3 virus and other common respiratory pathogens in children with human immunodeficiency virus infection. | 1988 | 2833716 | |
| parainfluenza virus type 3 meningitis in an 11-month-old infant. | 1988 | 2835742 | |
| the role of parainfluenza viruses in inspiratory difficulties in children. | viral findings were prospectively studied in middle and lower respiratory tract infections in 449 hospitalized children during a 12-month follow-up period. a viral aetiology was found in 30 of the 65 children (46%) with inspiratory difficulties. parainfluenza viruses were the infective agents in 24 of the 30 cases with viral diagnoses (80%), type 2 being the most prominent. there were 38 cases of parainfluenza infections, type 2 being the infective agent in 58% of the 24 cases of parainfluenza i ... | 1988 | 2835881 |
| protective effects of monoclonal antibodies against parainfluenza virus type 3-induced brain infection in hamsters. | a collection of monoclonal antibodies (mabs) against the haemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins of parainfluenza virus type 3 (piv3) was used for passive immunization of intracerebrally infected newborn hamsters. a significant degree of protection was obtained with mabs against both the hn and f viral proteins, but no individual antibody conferred complete protection against disease. mabs against different epitopes provided different degrees of protection. a mab to the se ... | 1988 | 2836547 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| survey on the prevalence of neutralizing antibodies to bovine viral diarrhea (bvd) virus, bovine herpes virus type 1 (bhv-1) and parainfluenza virus type 3 (pi-3) in ruminants in the djibouti republic. | 1988 | 2838248 | |
| live viral vaccines for respiratory and enteric tract diseases. | in its programme for accelerated development of vaccines for viral respiratory and enteric tract diseases the who has assigned a very high priority to respiratory syncytial virus (rsv), parainfluenza viruses and rotaviruses. there is also some interest in alternative approaches to immunization against influenza viruses because of the failure of inactivated vaccines to provide complete and reasonably durable immunity. current attempts to develop satisfactorily attenuated viruses for use in preven ... | 1988 | 2838984 |
| effects of infection with parainfluenza-3 virus and infectious bovine rhinotracheitis virus on neutrophil functions in calves. | calves were challenge exposed in separate experiments with parainfluenza-3 (pi-3) virus or infectious bovine rhinotracheitis (ibr) virus. blood neutrophils were assayed for functional activity every other day for at least 3 weeks by random migration, staphylococcus aureus ingestion, antibody-dependent cell-mediated cytotoxicity, cytochrome-c reduction, iodination, and native chemiluminescence. exposure to pi-3 virus resulted in a brief febrile response and no other clinical signs. alterations in ... | 1988 | 2840008 |
| effect of dietary selenium and vitamin e on the primary and secondary immune response in lambs challenged with parainfluenza3 virus. | this study was conducted to determine the effect of dietary selenium (se) and vitamin e (vit e) on various blood characteristics and the primary and secondary humoral immune response of lambs challenged with parainfluenza3 virus (pi3 v). treatments included: 1) +se/+vit e, 2) -se/+vit e, 3) +se/-vit e and 4) -se/-vit e. the basal diet (-se/-vit e) was deficient in se and vit e. sodium selenite (.2 mg se/kg diet) and alpha-tocopherol acetate (20 mg vit e/kg diet) were added to +se and +vit e diet ... | 1988 | 2840427 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the l protein. | the sequence of the gene encoding the l protein of the human parainfluenza 3 virus was determined by direct dideoxy sequence analysis of the genomic 50 s rna and confirmed by molecular cloning and sequence analysis of recombinant clones. a series of three overlapping clones was generated by primer extension using genomic 50 s rna as the template. these clones originate within the 5' end of the hemagglutinin-neuraminidase gene, span the entire l gene, and extend into the extracistronic 5' end of ... | 1988 | 2841798 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| current approaches to the development of vaccines effective against parainfluenza viruses. | infection of infants and children with parainfluenza viruses can lead to serious lower respiratory tract disease. in order to review new information on the genetic organization, epidemiology, and immunobiology of these viruses and of respiratory syncytial virus, who, in conjunction with the national institute of allergy and infectious diseases, held a workshop in bethesda, md, usa, 4-5 may 1987. new data on the immunobiology and epidemiology of human parainfluenza 3 virus were presented that sho ... | 1988 | 2844427 |
| levamisole-induced attenuation of alveolar macrophage dysfunction in respiratory virus-infected calves. | a luminol-amplified chemiluminescence (cl) assay was used to evaluate the effect of levamisole on the metabolic activity of pulmonary alveolar macrophages (pam) from parainfluenza-3 (pi-3) and infectious bovine rhinotracheitis (ibr) virus-infected calves. using selective beta-adrenergic agonists and antagonists, beta 1-adrenoceptors were shown to inhibit the pam reactive oxygen species-dependent cl response. beta 2 adrenoceptors were apparently not important in the regulation of cl in pam of con ... | 1988 | 2844684 |
| rabbit model for mucosal immunity in the bowel: i. establishment of virus-infected ileal loops. | an animal model was designed for use in studies of initial cellular immune responses to virus infection of the intestinal mucosa. the animal chosen was the new zealand white rabbit and the mucosal site the subterminal ileum, isolated in a thirty-vella loop. the antigen used was parainfluenzavirus type 3, which would normally be destroyed by bile salts if ingested. loops approximately 20 cm in length, each containing at least one peyer's patch, were exteriorised through left paramedian stomata. a ... | 1988 | 2844978 |
| nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983. | we have sequenced the coding and noncoding regions of the hemagglutinin-neuraminidase (hn) genes of six clinical strains of human type 3 parainfluenza virus (piv3) isolated between 1973 and 1983, and compared them to the prototype 1957 strain. sequence variability does not result from the accumulation of mutations over time, but represents genetic heterogeneity in hn genes within the piv3 population. most of the nucleotide diversity occurs in the 5' noncoding sequences, exclusive of regions supp ... | 1988 | 2827373 |
| role of individual glycoproteins of human parainfluenza virus type 3 in the induction of a protective immune response. | affinity-purified hemagglutinin-neuraminidase (hn) and fusion (f) glycoproteins of human parainfluenza virus type 3 (p13 virus) were used to investigate their role in the induction of a protective immune response following immunization of hamsters. the efficacy of immunization with the glycoprotein antigens was tested by challenge infection. results of virus recovery from lungs and trachea demonstrated that although immunization with hn or f alone induced an antibody response to the respective g ... | 1988 | 2828674 |
| immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses. | peripheral blood mononuclear cells (pbmc) from calves infected with bovine herpesvirus type 1 (bhv1) or parainfluenza 3 virus (pi3) were cultured in vitro in the presence of inactivated specific antigen presented on mdbk cells. in the presence of inactivated antigen, pbmc from both bhv1-infected and control calves produced interferon (ifn)-alpha in 24 hour cultures. altering the culture conditions did not result in the detection of immune-specific ifn produced by mononuclear cells from bhv1-infe ... | 1988 | 2848301 |
| interactions of a nonneutralizing igm antibody and complement in parainfluenza virus neutralization. | while many viruses activate the complement cascade directly, this is generally not a neutralizing event in the absence of antibody. we used a nonneutralizing igm monoclonal antibody to parainfluenza virus type 3 (piv3) hemagglutinin-neuraminidase (hn) to explore the role of antibody in complement-dependent neutralization of piv3. neither the antibody nor nonimmune guinea pig serum (gps) neutralized piv3 significantly, but a more than 100-fold reduction in titer was found when antibody and gps we ... | 1988 | 2849234 |
| antibodies to parainfluenza virus type 3 in minnesota swine. | 1988 | 2849496 | |
| [etiology of pneumonia in children]. | 1988 | 2851127 | |
| [serologic survey of rinderpest, ibr-ipv, rsb, pi3 and bvd-md viruses in cattle in shaba and western zaire]. | 1988 | 2851152 | |
| effect of respiratory infections caused by bovine herpesvirus-1 or parainfluenza-3 virus on bovine alveolar macrophage functions. | calves, 90 to 130 days old, were inoculated with bovine herpesvirus-1 (bhv-1) or parainfluenza-3 (pi-3) virus. pulmonary lavage specimens obtained from calves before virus inoculation contained 98% alveolar macrophages (am) and 1% neutrophils. six days after inoculation, the mean percentage of neutrophils in lavage specimens had significantly increased to 7.9 +/- 6.0% in bhv-1-inoculated calves and to 18.3 +/- 9.9% in pi-3 virus-inoculated calves, reflecting viral-induced pulmonary inflammation ... | 1988 | 2851951 |
| fatal pneumonia caused by parainfluenza type 3 virus. | 1988 | 2854685 | |
| bovine parainfluenza-3 virus proteins recognized by antibodies of aerosol-exposed calves. | bovine parainfluenza-3 (bpi-3) virus proteins were radiolabeled with [35s]methionine. control serum containing antibodies to bpi-3 virus obtained from calves that had been immunized by aerosol exposure and by im inoculation and sera of cattle that were exposed to bpi-3 virus by aerosol only were used to immunoprecipitate bpi-3 virus proteins. the immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. viral proteins with estimated molecular weights of 72,00 ... | 1988 | 2854712 |
| parainfluenza 3 infections. | 1988 | 2854823 | |
| [pathogenicity of parainfluenza virus type 3 in mice]. | 1988 | 2855381 | |
| parainfluenza-3 virus-induced enhancement of histamine release from calf lung mast cells--effect of levamisole. | clinically healthy calves were divided into five groups. group 1 served as control; group 2 received levamisole (lev), 3 mg/kg, s.c.; group 3 was aerosolized with parainfluenza-3 virus (pi-3); group 4 received lev and pi-3 and group 5 was inoculated with pasteurella haemolytica. they were killed 6 days after virus exposure or 5-6 days after bacterial inoculation. lung mast cells were prepared by enzymatic treatment. mast cell histamine (hist) release was assayed spectrofluorometrically. total hi ... | 1988 | 2463373 |
| differences in bovine parainfluenza 3 virus variants studied by sequencing of the genes of viral envelope proteins. | by determining gene nucleotide sequences we compared the primary structures of the membrane (m), fusion (f), and hemagglutinin-neuraminidase (hn) proteins of bovine parainfluenza 3 virus strains, m, sc, and mr which are substrains derived from a wild strain yn. the m and sc viruses are indistinguishable in having very weak hemagglutination (ha) and neuraminidase (na) activities, but m virus' syncytium-inducing (si) activity is considerably higher than that of the sc virus. however, the results s ... | 1988 | 2829426 |
| molecular cloning and characterization of six genes, determination of gene order and intergenic sequences and leader sequence of mumps virus. | mrna isolated from mumps virus-infected vero cells was converted into cdna and cloned into the psti site of the plasmid pbr322. after screening with 32p-labelled cdna synthesized from poly(a)+ rna of uninfected or mumps virus-infected vero cells, five different groups of virus-specific clones were obtained. the virus specificity of the clones was confirmed by northern blot analysis, in which the cdna inserts from the five different groups hybridized to mrnas of about 2100, 1500, 1450, 2000 and 2 ... | 1988 | 3183630 |
| sequence determination of the mumps virus hn gene. | the hemagglutinin-neuraminidase protein (hn) of mumps virus was purified by immunoaffinity chromatography and fragmented by the combined action of cnbr and trypsin. the resulting peptides were separated by hplc and sequenced by automated edman degradation. using this hn-specific amino acid sequence data, a degenerate oligonucleotide was produced and subsequently used to screen a mumps virus cdna library to isolate hn-specific clones. the complete nucleotide sequence of the hn gene was determined ... | 1988 | 3369084 |
| effect of cloned human interferon-alpha 2a on bovine parainfluenza-3 virus. brief report. | pretreatment of bovine turbinate (btu) cells with cloned human interferon (ifn)-alpha 2a reduced the yield of infectious bovine parainfluenza-3 virus (pi-3 v). intracellular synthesis of hn glycoprotein was reduced on postinfection day 1 (pid 1), but it recovered to normal levels subsequently. however, reduction of this protein persisted in the released virus through pid 2. thin section electron microscopy demonstrated a drastically reduced release of mature virions and an accumulation of viral ... | 1988 | 2829793 |
| intranasal immunization of hamsters with envelope glycoproteins of human parainfluenza virus type 3. | envelope glycoproteins of human parainfluenza virus type 3 (piv-3) were selectively solubilized with n-octyl beta-d-glucopyranoside and reconstituted into lipid vesicles by dialysis of the detergent. the efficacy of the glycoprotein preparation as a subunit vaccine when administered to hamsters intranasally or subcutaneously was compared. animals receiving four intranasal immunizations with 5 micrograms of the glycoprotein preparation were completely resistant to challenge infection. only partia ... | 1988 | 2831281 |
| attenuation of bovine parainfluenza virus type 3 in nonhuman primates and its ability to confer immunity to human parainfluenza virus type 3 challenge. | bovine parainfluenza virus type 3 (piv-3) was evaluated as a candidate live-virus vaccine to protect against infection with human piv-3. the level of replication of bovine and human piv-3 and the efficacy of immunization with bovine piv-3 in protecting against subsequent challenge with human piv-3 was evaluated in nonhuman primates. the duration and magnitude of replication of human and bovine piv-3 in the upper and lower respiratory tracts of new world monkeys was similar, and animals infected ... | 1988 | 2831282 |
| immunization with vaccinia virus recombinants that express the surface glycoproteins of human parainfluenza virus type 3 (piv3) protects patas monkeys against piv3 infection. | patas monkeys (eryphrocebus patas) were immunized intradermally with two vaccinia virus recombinants that individually express the hemagglutinin-neuraminidase glycoprotein or the fusion glycoprotein of human parainfluenza virus type 3 (piv3). these immunizations induced a high titer of piv3 serum-neutralizing antibodies. at 1 month after immunization, monkeys were challenged intratracheally with piv3. subsequent virus replication was reduced in these monkeys by 3.2 log10 and 1.9 log10 (mean peak ... | 1988 | 2831389 |
| contribution to laboratory diagnosis of mumps and parainfluenza. | specific igm and igg antibodies to mumps virus (mv) were detected in sera of mumps-patients by elisa in agreement with the results obtained by indirect immunofluorescence (if). of given sera 37.5% contained igm reacting in indirect elisa also with the antigens of parainfluenza virus (piv) t3. in all patients with respiratory illness over 2 years of age, the significant increase of antibodies to piv in haemagglutination inhibition (hi) test was in good correlation with serum igm and igg antibody ... | 1988 | 2906222 |
| sequence analysis of the mumps virus mrna encoding the p protein. | the nucleotide sequence of the mumps virus phospho- or polymerase-associated (p) protein mrna has been determined by sequencing a full-length cdna clone and confirmed by partially sequencing the mrna and the genome. the mrna contains 1311 nucleotides excluding the poly(a) and encodes a protein of 390 amino acids with a calculated molecular weight of 41,574. three small polypeptides were seen in in vitro translation of viral mrna and hybrid-selected p mrna, possibly representing internal initiati ... | 1989 | 2922928 |
| the effect of parainfluenza 3 infection on guinea pig basophil and lung mast cell histamine release. | the guinea pig infected with parainfluenza 3 (p-3) has provided an animal model to study mechanisms of virus-induced asthma and airway hyperreactivity. evidence to identify the mechanisms by which p-3 infection enhances airway hyperreactivity, however, has not been established. the present study evaluated the effect of p-3 infection on histamine release (hr) from isolated peripheral blood guinea pig basophils and lung mast cells. basophil hr was determined in animals made basophilic with 13 dail ... | 1989 | 2466427 |
| identification of amino acids recognized by syncytium-inhibiting and neutralizing monoclonal antibodies to the human parainfluenza type 3 virus fusion protein. | neutralizing monoclonal antibodies specific for the fusion (f) glycoprotein of human parainfluenza type 3 virus (piv3) were used to select neutralization-resistant antigenic variants. sequence analysis of the f genes of the variants indicated that their resistance to antibody binding, antibody-mediated neutralization or to both was a result of specific amino acid substitutions within the neutralization epitopes of the f1 and f2 subunits. comparison of the locations of piv3 neutralization epitope ... | 1989 | 2474672 |
| presence of an anti-viral factor in peritoneal dialysis effluent. | viral peritonitis is an exceptionally rare occurrence in peritoneal dialysis. in fact, up to now, only one case report has been documented in the literature. in a prospective study, peritoneal dialysis effluent (pde) was specifically cultured for the following viruses: the herpes group of viruses, including herpes simplex types i (hsv) and ii, cytomegalovirus (cmv) and varicella-zoster (v-z), and the enteroviruses group including coxsackie b-5 (cox b), echo, enterovirus and polio. cultures were ... | 1989 | 2476580 |
| effects of bovine respiratory disease viruses and isoprinosine on bovine leukocyte function in vitro. | peripheral blood mononuclear cells obtained from 4- to 6-month-old-calves were inoculated in vitro with bovine herpesvirus-1, parainfluenza-3, or bovine virus diarrhea viruses. no increase in infectious virus progeny was observed; however, the viruses were detected in the cells for at least 96 h post-infection without any significant reduction in cell viability. the three viruses, either alone or in combination, suppressed phytohemagglutinin-induced proliferation of the mononuclear cells. the gr ... | 1989 | 2477940 |
| identification and partial purification of the fusion protein of avian paramyxovirus type 3. | the fusion protein of an avian paramyxovirus-3 from turkeys was detected as a poorly-labelled 56k 3h-glucosamine band. after immunopurification by a monoclonal antibody it elicited antisera which inhibited haemolysis but not haemagglutination. | 1989 | 2481433 |
| defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell. | a defective transport of bovine parainfluenza-3 virus (pi-3v) hemagglutinin-neuraminidase (hn) glycoprotein was evidenced in interferon (ifn)-treated bovine turbinate (btu) cells. indirect immunofluorescence performed with monoclonal antibody to pi-3 hn glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of ifn-treated cells. untreated, infected control cells had a generalized widespread fluorescence. unfixed control cells showed a uniform surface fluorescence in ... | 1989 | 2482015 |
| protection of lambs against experimental pneumonic pasteurellosis by transfer of immune serum. | passive protection of specific pathogen-free lambs against experimental pasteurellosis was achieved using antisera from conventionally reared sheep which were either convalescent from experimental pneumonia or inoculated with pasteurella haemolytica a2 vaccines. the complete immune sera, or immunoglobulin-rich fractions prepared from them, when administered separately or together provided 94-100% protection of recipients compared to control lambs. antibodies to p. haemolytica in donor sera were ... | 1989 | 2527437 |
| enzyme immunoassays for detection of igg and igm antibodies to parainfluenza types 1, 2 and 3. | quantitative enzyme immunoassays for parainfluenza type 1, 2 and 3 igg antibodies were developed. serum specimens were tested at a single dilution of 1:1000 and results expressed in units by the use of a standard curve. the unit values correlated well with titres obtained by testing the same specimens in serial dilutions. all serum pairs with significant titre rises also showed significant rises in unit values. parainfluenza igg and igm serology was evaluated in 66 patients with a proven parainf ... | 1989 | 2536382 |
| bovine herpesvirus-1 and parainfluenza-3 virus interactions: clinical and immunological response in calves. | calves infected with bovine herpesvirus-1 (bhv-1) or both bhv-1 and parainfluenza-3 virus (piv-3) developed clinical signs including fever, cough, and nasal and ocular discharges. animals infected with both viruses appeared more depressed and showed higher rectal temperature, while calves inoculated with piv-3 alone had a very mild clinical disease. both bhv-1 and piv-3 were recovered from nasal secretions up to six to eight days postinoculation. however, the virus titers were lower in calves wi ... | 1989 | 2536581 |
| virus-induced enhancement of arachidonate metabolism by bovine alveolar macrophages in vitro. | virus infection of alveolar macrophages both in vivo and in vitro has been associated with a variety of changes in cellular function. some of these changes are identical to the effects that arachidonate-derived mediators, prostaglandins, leukotrienes, and hydroxyeicosatetraenoic acids, have on macrophage function. virus infection of macrophages has been previously shown to increase the output of some arachidonate metabolites, most notably pge2. however, the effect of virus infection on arachidon ... | 1989 | 2539424 |
| reversal of virus-induced alveolar macrophage bactericidal dysfunction by cyclooxygenase inhibition in vitro. | virus infection of alveolar macrophages (am) both in vivo and in vitro has been associated with a decreased ability of these cells to kill bacteria, together with enhanced production of metabolites of arachidonic acid. these metabolites, especially pge2, may be inhibitory to some phagocyte functions. primary cultures of bovine am obtained by bronchoalveolar lavage of normal cattle were infected in vitro with parainfluenza-3 (pi3 virus) virus. killing of staphylococcus epidermidis by am was deter ... | 1989 | 2539425 |
| analysis of the in vitro antiviral activity of certain ribonucleosides against parainfluenza virus using a novel computer aided receptor modeling procedure. | the in vitro antiviral activity of 28 nucleosides against the parainfluenza virus type 3 has been analyzed by using a novel computer aided receptor modeling procedure. the method involves an extensive modification of our earlier work (ghose, a. k.; crippen, g. m. j. med. chem. 1985, 28, 333). it presents a more straightforward algorithm for the steps that suffered from subjectivity in the earlier method. the method first determines the possible low-energy conformations of the nucleosides, and as ... | 1989 | 2539476 |
| a neutrophil-derived antiviral protein: induction requirements and biological properties. | polymorphonuclear neutrophilic granulocytes (pmn) have been implicated as playing a role in antiviral defense. in addition to having phagocytic and cytotoxic activities, pmn may produce an antiviral substance with interferon (ifn)-like activity. the product, for which the name polyferon (pf) has been coined, is produced upon direct encounter of pmn with bovine herpesvirus 1 (bhv-1)-infected bovine cells or membranes thereof. exposure to purified virus only does not induce pf. the intimate intera ... | 1989 | 2539494 |
| expression of the fusion glycoprotein of human parainfluenza type 3 virus in insect cells by a recombinant baculovirus and analysis of its immunogenic property. | the fusion (f) glycoprotein of human parainfluenza type 3 (pi3) virus was produced in insect cells using a baculovirus expression vector (pacym1). the recombinant glycoprotein was identified by its reactivity with specific monoclonal and polyclonal antibodies and showed an apparent molecular mass of 70 kda. although the fusion protein was found on the infected cell surface, it did not appear to be proteolytically cleaved to f1 and f2 subunits. immunization of hamsters with the recombinant protei ... | 1989 | 2539707 |
| bronchial hyperreactivity induced by parainfluenza 3 is partly prevented by atropine. | 1989 | 2540646 | |
| efficacy of viral components of a nonabortigenic combination vaccine for prevention of respiratory and reproductive system diseases in cattle. | efficacy and safety of components of an im-administered vaccine for prevention of infectious bovine rhinotracheitis virus (ibrv), parainfluenza type-3 (pi-3) virus, bovine viral diarrhea virus (bvdv), and respiratory syncytial virus (rsv) infections and campylobacteriosis and leptospirosis were evaluated in cattle, including calves and pregnant cows. challenge of immunity tests were conducted in calves for ibrv, pi-3 virus, or bvdv vaccinal components. all inoculated calves developed serum-neutr ... | 1989 | 2542202 |
| [mechanism of penetration of human type 3 parainfluenza virus into monkey kidney cells]. | the mechanism of human parainfluenza type 3 virus penetration into monkey kidney cells was studied by morphological and biochemical methods. the results of electron microscopic studies permit a conclusion that the virus penetrates into the cells by the mechanism of receptor endocytosis. analysis of subvirus structures in cytosole revealed two types of particles: nucleocapsids and structures of a larger size and lower buoyant density containing, in addition to np protein, matrix (m) protein. it i ... | 1989 | 2543139 |
| epidemiology of parainfluenza virus type 3 in england and wales over a ten-year period. | we have analysed data on respiratory syncytial (rs) and parainfluenza type 3 (pf3) viruses reported to the communicable disease surveillance centre, london, over the period 1978-87. these confirm the annual winter epidemic of rs virus and show that, in england and wales, pf3 is a summer infection with regular yearly epidemics. | 1989 | 2544442 |
| synthesis of 3-deazaneplanocin a, a powerful inhibitor of s-adenosylhomocysteine hydrolase with potent and selective in vitro and in vivo antiviral activities. | the neplanocin a analogue 3-deazaneplanocin a (2b) has been synthesized. a direct sn2 displacement on the cyclopentenyl mesylate 3 by the sodium salt of 6-chloro-3-deazapurine afforded the desired regioisomer 4 as the major product. after deprotection, this material was converted to 3-deazaneplanocin a in two steps. x-ray crystallographic analysis confirmed the assigned structure. consistent with its potent inhibition of s-adenosylhomocysteine hydrolase, 3-deazaneplanocin a displayed excellent a ... | 1989 | 2544721 |
| acute inflammatory lung injury retards pulmonary particle clearance. | the deposition, retention, and clearance of inhaled cobalt oxide particles from the lungs of calves with acute inflammatory lung injury induced by parainfluenza-3 virus (pi-3) were examined. acute pulmonary inflammation was induced by nebulization with 10(9) tcid50 of pi-3 virus on two successive days, and animals were subsequently exposed to an aerosol of particulate cobalt oxide (geometric mean diameter 0.54-0.65 microns) seven days post-virus infection (dpi). pulmonary lesions at 7 dpi were t ... | 1989 | 2547712 |
| syncytium formation by recombinant vaccinia viruses carrying bovine parainfluenza 3 virus envelope protein genes. | the highly syncytium-inducing m strain and the weakly syncytium-inducing sc strain of bovine parainfluenza 3 virus differ by a single amino acid substitution in each of the hemagglutinin-neuraminidase (hn) and membrane (m) proteins, while their fusion (f) proteins are identical (t. shioda, s. wakao, s. suzu, and h. shibuta, virology 162:388-396, 1988). we constructed recombinant vaccinia viruses which express separately the m virus hn (vac-mhn), sc virus hn (vac-schn), m virus m (vac-mm), sc vir ... | 1989 | 2547990 |
| [comparative study of the hemolytic activity of ortho- and paramyxoviruses]. | a comparative study of hemolytic activity of influenza type a, b, and c viruses, human parainfluenza type 3, and sendai virus showed the pattern of ph-dependence and the nature of the curve to differ not only for different viruses under study but also for different erythrocyte species. studies of virus-induced hemolysis of influenza c virus demonstrated that, depending on the erythrocyte species used, it had common properties both with influenza types a and b viruses and with paramyxoviruses. | 1989 | 2548343 |
| association of parainfluenza virus type 3 infection with allograft rejection in a liver transplant recipient. | 1989 | 2549495 | |
| the serological response to foot-and-mouth disease vaccination is not affected by simultaneous infectious bovine rhinotracheitis/adenovirus/parainfluenza 3 vaccination. | young calves were simultaneously vaccinated by subcutaneous route against foot-and-mouth disease (fmd) and against infectious bovine rhinotracheitis/adenovirus/parainfluenza-3 (ibr/adeno/pi-3) by intranasal route. the serological response against the 3 fmd virus types of the fmd vaccine was clearly positive. there was no significant difference between results of simultaneous fmd and ibr/adeno/pi-3 vaccination and fmd vaccination only. | 1989 | 2549906 |
| cell lines for growth of sheep viruses. | 1989 | 2549933 | |
| prevalence of antibodies in goats in north-eastern brazil to selected viral and bacterial agents. | 1989 | 2552630 | |
| parainfluenza 3 vaccination of sheep. | outbreaks of pneumonia associated with pasteurella haemolytica have occurred in sheep in the area of perthshire served by this practice, and on some farms the disease has been an important annual cause of loss. serological evidence was obtained that parainfluenza 3 (pi3) virus might be implicated as a predisposing factor to pasteurellosis. a live attenuated pi3 virus vaccine licensed for use in cattle was given intranasally to ewes on one farm. many sheep seroconverted and outbreaks of pneumonia ... | 1989 | 2556831 |
| epidemiologic survey of bovine diseases in suriname. | a seroepidemiologic survey of cattle diseases was undertaken in suriname in 1985 to help assess the livestock disease situation in that country. the six diseases covered by the survey were bovine coronavirus infection, bovine rhinotracheitis, bovine virus diarrhea, brucellosis, parainfluenza-3 infection, and respiratory syncytial virus infection. the results indicated relatively low prevalences of these diseases compared to the prevalences found in most developed countries. the reasons for this ... | 1989 | 2558753 |
| [serum antibody titers against various viruses in idiopathic interstitial pneumonia]. | as the first step to investigate a possibility that viral infection is involved in the pathogenesis of idiopathic interstitial pneumonia (iip), serum antibody titers against various viruses were studied in 98 iip patients. the patients were positive for herpes simplex virus, cytomegalovirus, rubella virus, parainfluenza virus 3, ebv-vca igg and adeno virus 1, which were also assessed in 45 normal subjects. as results, iip patients showed higher titers of ebv-vca igg (p less than 0.01). when the ... | 1989 | 2559226 |
| studies of the effect of recombinant human-alpha 1 interferon on experimental parainfluenza type 3 virus infections of the respiratory tract of calves. | the effect of the administration of recombinant human interferon on the severity of clinical disease and the extent of pneumonic lesions in calves infected experimentally with bovine parainfluenza 3 (pi3) virus was studied in two experiments. in the first, three pairs of calves aged seven to 10 days were used; one of each pair was injected intramuscularly with 10(6) units of interferon/kg bodyweight for three consecutive days, and the other was left untreated. on the day after the first injectio ... | 1989 | 2559530 |
| an evaluation of four serological tests for the detection of antibodies to bovine parainfluenza virus type 3. | haemagglutination-inhibition (hi), virus neutralization (vn), haemolysis-inhibition (hli-1, hli-2) tests, and two new haemofusion-inhibition (hfi-1, hfi-2) tests, developed by us, were tested for the detection of antibodies to bovine parainfluenza virus type 3 (bpiv-3) in sera of 45 steers randomly selected from a herd naturally infected by bpiv-3. twelve seronegative animals were then vaccinated with formalin-inactivated vaccine and 15 days later tested by the above-mentioned assays. linear reg ... | 1989 | 2561663 |
| quantitative method of measuring anti-viral activity of peritoneal dialysis effluent. | we have previously documented the presence of anti-viral activity in the peritoneal effluent of uremic patients treated by peritoneal dialysis. anti-viral activity was determined by recording the inhibition of the cytopathic effect (cpe) of various viruses on vero cells in a semi-quantitative manner based on observer's subjective judgment of the degree of inhibition of cpe. this degree of inhibition is a measure of the anti-viral activity. although peritoneal dialysis effluent (pde) was consiste ... | 1989 | 2577401 |
| rapid detection of influenza virus infections in human fetal lung diploid cell cultures. | haemadsorbing foci were found in human fetal lung (hfl) diploid cell cultures 12 h after inoculation with influenza viruses a and b. the size and number of the foci were maximal after 48 h of incubation, being limited by production of an unidentified inhibitor. by contrast, inoculation with parainfluenza virus type 3 led to haemadsorption which increased during 10 days of incubation. for the detection of influenza viruses a and b maximum sensitivity was achieved by changing the medium, the day b ... | 1989 | 2663998 |
| the mumps virus nucleocapsid mrna sequence and homology among the paramyxoviridae proteins. | the nucleotide sequence of mumps virus nucleocapsid protein (np) mrna has been determined from two overlapping cdna clones and confirmed by partial sequencing of the mrna and the genome. the mrna contains 1844 nucleotides excluding poly(a) and encodes a protein of 553 amino acids with a calculated molecular weight of 61,792. a comparison of the mumps virus nucleocapsid protein sequence with that of other paramyxoviruses revealed a moderate degree of homology (33.1%) with the newcastle disease vi ... | 1989 | 2718626 |