Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation of cytopathogenic rotavirus from neonatal calves. | rotavirus was isolated in rolled calf kidney cultures from the intestinal contents of a calf suffering from diarrhoea. the cytopathic effect was demonstrated in native cultures as well as in stained preparations. the affected cells were elongated, became sickle-like in shape, disintegrated and detached from the monolayer. virus-specific direct immunofluorescence ran parallel, in both time and intensity, with the cytopathic effects. immuno-electron microscopy showed aggregated virus particles cor ... | 1979 | 232369 |
| comparison of hemagglutination-inhibition, complement-fixation and enzyme-linked immunosorbent assay for quantitation of human rotavirus antibodies. | the hemagglutination-inhibition test for detecting rotavirus antibody was evaluated by using simian rotavirus sa-11 as hemagglutinating antigen. results show that the test is as sensitive as either complement fixation or the enzyme-linked immunosorbent assay for detecting antibody to rotavirus in human sera. | 1979 | 232412 |
| analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus. | solid-phase enzyme immunoassays can be utilized to detect antigens directly in clinical specimens. however, a small number of stools which we tested for human rotavirus by enzyme-linked immunosorbent assay (elisa) were found to have nonspecific activity in the absence of rotaviral antigen. similar nonspecific activity was found in eight of eight sera which contained rheumatoid factor. this nonspecific activity was markedly reduced by pretreatment of the specimens with reducing agents, normal goa ... | 1979 | 232506 |
| rapid diagnosis of rotavirus infection: comparison of electron microscopy and enzyme linked immunosorbent assay (elisa). | 1979 | 232652 | |
| [immunoelectron microscopic observation of the incidence of rotaviruses in the stools of children with gastroenteritis (author's transl)]. | 1979 | 232672 | |
| comparison of methods for immunocytochemical detection of rotavirus infections. | rotavirus infections in intestinal tissues of animals or in tissue culture cells were detected by the immunocytochemical unlabeled soluble enzyme peroxidase antiperoxidase method. comparison of the immunofluorescence and peroxidase antiperoxidase immunological staining techniques revealed that the two methods are equally sensitive for detection of rotavirus-infected cells. the peroxidase antiperoxidase technique offers the advantages of negligible nonspecific staining reactions, the use of a sta ... | 1979 | 232694 |
| [epidemiological and clinical significance of rotoviruses]. | 1979 | 232752 | |
| bovine fetal inoculations with calf rotavirus. | the serological and histopathological responses of bovine fetuses to in utero inoculation with virulent and attenuated strains of the calf rotavirus (reovirus-like agent of neonatal calf diarrhea) are described. thirteen bovine fetuses, 63 to 190 days of gestation, were inoculated in utero with attenuated (three fetuses) or field strain virus (nine fetuses) or both (one fetus).serum-neutralizing antibody titers ranging from 1:16 to > 1:256 were detected in six of eight fetuses tested, demonstrat ... | 1979 | 232853 |
| viral intestinal infections of animals and man. | 1979 | 232863 | |
| antibodies to rotavirus infection in singapore children. | 1979 | 232890 | |
| [experiments to obtain and test a cell-culture rotavirus-precipitating antigen]. | the location of the rotaviral precipitating antigen and the possibility for its production from cattle rotaviral strains adapted on cell cultures of calf kidney were investigated. highest titer antigen was produced by concentration with ammonium sulfate simultaneously from the maintaining medium and from the cell monolayer. comparative studies on the antigenic and physico-chemical properties of the cell-cultural and the faeces rotaviral precipitating antigen were made. the identity of both antig ... | 1979 | 232949 |
| gel electrophoresis of rotavirus rna derived from six different animal species. | rotavirus rna prepared from calf, pig, mouse, deer, foal and dog-adapted human isolates was compared using polyacrylamide gel electrophoresis. reproducible differences in the rna migration patterns were found between all isolates. there were 11 clearly resolved segments in the pig, mouse and foal samples. the calf rotavirus rna and deer rotavirus rna separated into 9 bands and 10 bands, respectively. the dog-adapted human virus migrated in 12 bands, and this probably results from the complex pas ... | 1979 | 232982 |
| [detection of rotavirus antigen in stools by double-gel diffusion technique (author's transl)]. | 1979 | 233056 | |
| the isolation and cultivation of calf rotavirus in the republic of south africa. | calf rotavirus was cultivated and propagated in tissue culture from faeces of 3-week-old calves suffering from severe diarrhoea. criteria for viral involvement were: production of cytopathic effect in primary foetal calf kidney cells, specific fluorescence, and identification of the agent by means of electron microscopy. in a limited serological survey the majority of the cows on an infested farm were found to possess neutralizing antibodies to the local rotavirus strain. | 1979 | 233145 |
| rotavirus in diarrhoeic calves in iran. | 1979 | 233279 | |
| rotavirus infection in a domestic cat. | 1979 | 233280 | |
| rota virus infections of domestic animals and humans. | 1979 | 233385 | |
| [pediatric aspects of acute infectious diarrhea]. | 1979 | 233399 | |
| morphological and immunological studies of rotaviruses. (2) demonstration of two types of morphological units released from the capsid surfaces of rotaviruses. | 1979 | 233638 | |
| etiology of gastroenteritis among americans living in the philippines. | between december 1975 and june 1976, american military personnel with diarrhea who were seen at an outpatient clinic at clark air force base hospital were investigated to determine the etiology of their disease. enterotoxigenic escherichia coli were detected in 16% (18/115), rotavirus in 4% (4/111), salmonellae in 3% (3/115), shigellae in 2% (2/115) and giardia lamblia in 2% (3/152) of patients with diarrhea. thus from only 27% of the subjects studied were identifiable potential pathogenic agent ... | 1979 | 375723 |
| detection of antigens and igm antibodies for rapid diagnosis of viral infections: a who memorandum. | this memorandum describes recent progress in the development of simplified and rapid laboratory technology and reviews currently available methods for the direct detection of viral antigens in clinical material and/or quantification of specific igm antibody in acute serum specimens. particular emphasis is given to such infections as viral hepatitis, rotavirus gastroenteritis, viral infections of the skin, genital, and respiratory tracts, and rabies. in addition, the standardization and quality c ... | 1979 | 394872 |
| intestinal immunity and vaccine development: a who memorandum. | as part of the research component of the who diarrhoeal diseases control programme, a scientific working group met in august 1978 to review recent advances in knowledge of intestinal immunity, the application of genetic techniques in enteric vaccine development, the status of currently available immunizing agents against cholera, typhoid fever, and shigella dysentery, and the prospects for the development of new or improved vaccines against the well known and newly recognized agents such as rota ... | 1979 | 396051 |
| acute diarrheal infections in infants. i. epidemiology, treatment, and prospects for immunoprophylaxis. | epidemiologic differences that appear to be geographic or climatic actually relate more closely to socioeconomics and sanitation. regardless of etiology, the major management problems are those of dehydration and its sequelae. progress toward development of antibacterial and antiviral vaccines is discussed. next month the viral and bacterial agents that cause diarrheal infections will be reviewed. | 1979 | 511128 |
| an out-break of acute gastroenteritis due to rotavirus in an infant home. | in december 1976, an outbreak of acute infectious diarrhea occurred among infants who resided in an infant home in the city of sapporo. rotavirus infection was proved in 42 (90%) of 47 infants by serologic and/or electron microscopic examinations. out of 42 infected infants 38(90%) were clinically affected; diarrhea with or without vomiting in 27 (64%), vomiting without diarrhea in 6 (14%) and only febrile episode in 5 (12%). the remaining 4(10%) infants showed no symptoms. clinical manifestatio ... | 1979 | 220751 |
| rotavirus gastro-enteritis in northern nigeria. | 1979 | 220757 | |
| a survey for rotavirus antibodies in papua new guinea cattle. | 1979 | 220948 | |
| etiology of infantile enteritis in south africa. | infantile enteritis constitutes a major health problem in developing countries. several investigations into the etiology of this condition among various south african populations have been undertaken during the past few years. recent studies of black urban infants have revealed that salmonellae, shigellae, enterotoxigenic enterobacteriaceae, and rotaviruses play a relatively minor role in infantile enteritis. on the other hand, all studies, including a number performed several years ago, have de ... | 1979 | 221438 |
| rotavirus infection in adults. results of a prospective family study. | to study the epidemiologic and clinical features of rotavirus infections, we enrolled 98 families in a prospective study of diarrhea in households with newborn children. families were seen at three-month intervals and whenever ill. the mean follow-up period was 16.4 months. rotavirus infections were documented by electron microscopy of feces, indirect fluorescent-antibody assays in serum or both. the 43 infections identified in adults represented an attack rate of 0.17 per adult per year. ninety ... | 1979 | 221816 |
| rotavirus and coronavirus associated diarrhoea in domestic animals. | 1979 | 221870 | |
| quantitative observations on experimental reo-like virus (rotavirus) infection in colostrum-deprived calves. | ten calves were challenged with one of two strains of reo-like virus (rotavirus). changes in the daily faecal and urinary outputs were monitored and packed cell volume, plasma sodium, potassium and urea levels were measured. faeces were examined for the presence of rotavirus by direct electron microscopy and immunofluorescence in cultures of pk(15) cells. all calves excreted rotavirus in the faeces for several days. two calves remained clinically normal throughout the experiment, but in the rema ... | 1979 | 222036 |
| a rotavirus from kittens. | 1979 | 222038 | |
| epidemic viral gastroenteritis. | epidemic viral gastroenteritis is a significant world wide problem. in developed countries, gastroenteritis accounts for significant morbidity and loss of time from work; in the third world it is the leading cause of mortality among infants and children. recent technologic advances have been associated with an explosion of research activity. two virus groups, the norwalk-like agents and the rotaviruses, are currently accepted as causative agents of viral gastroenteritis in man. the problem of vi ... | 1979 | 222141 |
| diarrhea associated with rotavirus in rural guatemala: a longitudinal study of 24 infants and young children. | a population of 24 infants and young children followed prospectively during the first 3 years of life was studied for the occurrence of rotavirus infection by using enzyme-linked immunosorbent assay to detect virus in stools. infection with rotavirus was associated with 26 (14.2%) of 183 selected diarrheal episodes. twenty of the 24 infants and young children had diarrhea associated with rotavirus on at least one occasion and six had two such episodes. rotavirus infection was documented in over ... | 1979 | 222155 |
| [diagnosis of rotavirus by electron microscopy and the conjugated enzyme linked immunosorbent test (elisa)]. | 1979 | 222309 | |
| [rotaviruses: origin of acute gastroenteriteis in newborns and infants]. | 1979 | 222566 | |
| production of high-titer bovine rotavirus with trypsin. | titers of bovine rotavirus in excess of 10(9) immunofluorescent infectious units per ml of culture fluids have been produced, using trypsin treatment of the virus. infectivity of preparations of the virus can be increased with as little as 1 ng of trypsin per ml, with maximum increases of 1 to 2 log10 with 1 microgram of trypsin per ml. the virus grows to titers in excess of 10(5) immunofluorescent units per ml in mdbk, llc-mk2, ma-104, and hela cells. when mdbk cells are infected with a multipl ... | 1979 | 222801 |
| enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus. | infection of cell cultures with human rotavirus preparations was attempted and the effects of trypsin and low-speed centrifugation on antigen incorporation, as demonstrated by immunofluorescence and radioimmunoassay, were determined. in addition, the effect of viral aggregation on antigen incorporation was investigated by filtering viral preparations. four strains of human rotavirus were employed, and the results were compared to those obtained with two tissue culture-adapted animal rotaviruses. ... | 1979 | 222805 |
| diarrhea and rotavirus infection associated with differing regimens for postnatal care of newborn babies. | surveillance of 2,041 babies born during 4 winter months in one obstetric hospital in melbourne, australia, showed that 215 developed acute diarrhea during the first 2 weeks of life. babies requiring special care from birth had a high incidence of sporadic diarrhea (36%). the incidence of diarrhea among healthy full-term babies was low if they were "rooming-in" with their mothers (2 to 3%) but high if they were housed in communal nurseries (29%). the most important factor influencing incidence o ... | 1979 | 222807 |
| [virusenteritis in childhood (author's transl)]. | about 20% of infantile enteritis are caused by bacterial infections. the most cases of non bacterial infantile enteritis are supposed to be viral infections. arguments for the existence of enterotropic viruses arised the first time in 1943. since the middle of the fifties, various kinds of enteroviruses are known to be causative agents of infantile gastroenteritis. the same is true occasionally for some types of adenovirus. in 1973 the norwalk-agent was discovered provocing enteritis mainly in a ... | 1979 | 222939 |
| [viral and bacterial etiology of infantile gastroenteritis cases: clinical characterization]. | 1979 | 223230 | |
| rotaviruses in diarrheic feces of a dog. | 1979 | 223269 | |
| [rotavirus gastro-enteritis in infants]. | 1979 | 16106868 | |
| detection of viruses in avian faeces by direct electron microscopy. | a total of 151 specimens of turkey and chicken faeces and intestinal contents were examined for the presence of viruses by electron microscopy. viruses were detected in 48 of these specimens (32%). the most frequently observed viruses were rotaviruses and enterovirus-like particles. rotavirus infection was associated with outbreaks of diarrhoea in turkeys, but symptomless rotavirus infection was seen in broiler chickens. adeno-viruses and infectious bursal disease virus were also observed in tur ... | 1979 | 18770449 |
| isolation of rotaviruses from turkeys and chickens: demonstration of distinct serotypes and rna electropherotypes. | six isolates of rotavirus were made from turkeys and two from chickens. three of these required trypsin treatment for isolation and serial passage in cell cultures. the remainder were isolated without trypsin treatment. most of these viruses were isolated in chick embryo liver cell cultures from the faeces of birds aged under 1 week. in six of the eight instances, rotavirus isolation was associated with enteric disturbance, characterised by signs such as diarrhoea, poor or abnormal appetite, abn ... | 1980 | 18770274 |
| university-industry programs. | in the report by r. g. wyatt et al. "human rotavirus type 2: cultivation in vitro" (11 jan., p. 189), in the sentence describing the porcine rotavirus plaque reduction test (p. 190, column 3, line 16), the concentration of pancreatin in the agar overlay should have been given as "0.15 percent of 2.5 percen pantreatin 4 x n. f.; gibco." | 1980 | 17729852 |
| canine viral enteritis: prevalence of parvo-, corona- and rotavirus infections in dogs in the netherlands. | summary after a brief review of the present knowledge about canine viral enteritis, the role played by parvoviral, coronaviral and rotaviral infections in contagious diarrhoea in dogs in the netherlands is discussed. for this purpose a serologic survey, pathologic findings in dogs, and the demonstration of parvoviral antigen with an immunofluorescence test and with a newly developed haemadsorption-elution-haemagglutination assay (heha) are presented. it is concluded that infections with ca ... | 1980 | 22039978 |
| weak susceptibility of rotavirus to bovine interferon in calf kidney cells. | bovine, virus-induced interferon was titred in primary embryonic calf kidney cells, using three challenge viruses: reovirus type 3, vesicular stomatitis virus (serotype indiana), and a french isolate of bovine rotavirus (tr) adapted to grow in tissue culture. rotavirus repeatedly displayed a very weak susceptibility to interferon as compared to the other two viruses. | 1980 | 6155896 |
| negative contrast electron microscopic techniques for diagnosis of viruses of veterinary importance. | negative contrast electron microscopy (ncem) was utilized as a routine tool in the diagnosis of viral infections of domestic and wild animals. viruses identified by this technique were observed in infected culture systems or clinical specimens from several species including horses, cattle, sheep, dogs, cats, pigs, deer, rocky mountain bighorn sheep, antelope, and several avian species. viruses were identified by ncem based on their size, morphology, and symmetry and consisted of adenoviruses, he ... | 1980 | 6157508 |
| the action of alcohols on rotavirus, astrovirus and enterovirus. | 1980 | 6182233 | |
| hospital acquired rotavirus infection in adults. who is at risk? | 1980 | 6182234 | |
| studies on neonatal calf diarrhoea caused by rotavirus: transmission of the disease and attempted vaccination of colostrum-deprived calves. | mild to severe scouring could be produced in colostrum-deprived calves with tissue culture-adapted rotavirus and feacal material from field cases of calf diarrhoea. the feaces of experimentally infected calves contained rotavirus for at least 3 days. pathogenic bacteria were presented in one calf only and this calf also showed the most severe gastroenteritis. eight calves were vaccinated with a live rotaviral calf diarrhoea vaccine and subsequently challenged with infective rotavirus. mild scour ... | 1980 | 6256698 |
| effects of environmental variables and soil characteristics on virus survival in soil. | because of the increasing emphasis placed upon land application as a means of wastewater disposal, it is important to evaluate the influences of different factors upon virus survival in soil. the objective of this study was to measure the effects of various environmental variables on virus persistence. test samples of soil were placed in vials, and the soil was wetted with suspensions of virus in either distilled water, unchlorinated secondary sewage effluent, or mixtures of effluent and water. ... | 1980 | 6257161 |
| rotavirus concentration from cell culture harvests: trypsin treatment followed by hydroextraction. | an 80- to 150-ml amount of calf or simian rotavirus-containing cell culture harvests of ma-104 cells were treated with 50 microgram of trypsin per ml and hydroextracted overnight (4 degrees c) with polyethylene glycol 6,000. the concentrate was resuspended in 8 to 10 ml of tryptose phosphate broth and plaque assayed. between 85 and 97% of the input virus could be recovered with a concentration of up to 15-fold. | 1980 | 6257163 |
| summer diarrhoea in african infants and children. | of 70 black south african infants and children with acute summer diarrhoea, 30 (43%) were infected with enteropathogenic serogroups of escherichia coli (epec), 13 (19%) with enterotoxigenic gram-negative bacilli, 12 (17%) with salmonella sp., 6 (9%) with shigella sp., and 3 (4%) with rotaviruses. 13 (19%) patients were infected simultaneously with more than one enteropathogen, and no pathogen was detected in 22 (31%). in addition, 6 (15%) of 41 unselected patients were excreting campylobacter fe ... | 1980 | 6257185 |
| the clinical response of gnotobiotic calves, pigs and lambs to inoculation with human, calf, pig and foal rotavirus isolates. | the infectivity, pathogenicity and immunogenicity of 5 human, 6 calf, 2 pig and 2 foal rotavirus isolates were studied in gnotobiotic calves, piglets and lambs. three of the human isolated produced subclinical infection in newborn gnotobiotic piglets and the piglets developed neutralising antirotavirus antibody. when challenged with pig rotavirus 2 weeks later, the piglets did not develop diarrhoea, but rotavirus was detected in the faeces. in contrast, piglets inoculated with the other 2 human ... | 1980 | 6257218 |
| [seroepidemiologic survey of the spread of rotavirus infection in a milan population]. | the increasing evidence of involvement of rotaviruses in the etiology of viral gastroenteritis prompted us to carry on a seroepidemiological survey on the population of milan. the aim was to get informations on the extension of the circulation of these agents in our population. utilising a commercial antigen prepared with the "oslo" bovine strain, c.f.a. were titrated in sera from 575 healthy subjects (age 0 to > 60 years). of them 232 were children under 4 years of age homogeneously distributed ... | 1980 | 6257252 |
| [serodiagnosis of rotavirus infections in gastroenteritis of infants]. | we report the use of the line immunoelectroosmophoresis (lieop), a modification of the immunoelectroosmophoresis (ieop), for the serodiagnosis of rotavirus infection, in comparison with the complement fixation test. we tested 17 paired and 29 single sera from hospitalized children affected by acute gastroenteritis; rotavirus had been searched in the stools both by electron microscopy and ieop. the lieop showed seroconversion in all the 11 cases with positive stools and also in 5 out of 5 patient ... | 1980 | 6257253 |
| [cross-over electrophoresis on cellulose acetate. a new method for rapid diagnosis of rotavirus infection]. | a new application of crossing-over electrophoresis on cellulose acetate (c.o.e.) in diagnosis of rotavirus infections is described. the results are compared with those obtained in electron microscopy (e.m.). the main reasons for application of c.o.e. to routine virological diagnosis of rotavirus infections are exposed. | 1980 | 6257254 |
| a two-year study of bacterial, viral, and parasitic agents associated with diarrhea in rural bangladesh. | enteric pathogens associated with diarrhea were studied for two years at a diarrhea treatment center in rural bangladesh. enterotoxigenic escherichia coli (etec) was the most frequently identified pathogen for patients of all ages. rotavirus and etec were isolated from approximately 50% and approximately 25%, respectively, of patients less than two years of age. a bacterial or viral pathogen was identified for 70% of these young children and for 56% of all patients with diarrhea. most etec isola ... | 1980 | 6257795 |
| rotavirus infections in calves in dairy herds. | neonatal calf diarrhoea was studied in 115 calves of one dairy herd from january 1976 to june 1977. two syndromes could be distinguished: a mild and short lasting 'early diarrhoea' within the first three days of life and a usually more severe 'late diarrhoea' from the fourth to the 14th day of life. the latter type of diarrhoea occurred almost exclusively during the first half of the year. thirty-four out of 45 calves with late diarrhoea excreted rotaviruses, whereas only one of 34 calves with e ... | 1980 | 6258202 |
| rotavirus infections in calves: efficacy of oral vaccination in endemically infected herds. | a commercially available modified live reovirus-like (rotavirus) vaccine proved innocuous in calves deprived of colostrum and protected one out of three calves against challenge 72 h after vaccination. the vaccine was evaluated in two dairy herds in the 1977 calf season. no significant differences were observed in the incidence rates or severity of undifferentiated neonatal calf diarrhoea or rotavirus-associated late diarrhoea between calves given a placebo (76) and vaccinated (74) calves in the ... | 1980 | 6258203 |
| [rota- and coronaviruses associated with neonatal calf diarrhea: identification of viruses by immunoelectron microscopy, histological and clinical studies]. | 1980 | 6258224 | |
| detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. | 1980 | 6258286 | |
| [etiologic and epidemiologic study of viral etiology of diarrhea in children]. | 1980 | 6258339 | |
| [demonstration of enteropathogenic escherichia coli strains and rotaviruses in fecal samples from calves with diarrhea]. | 1980 | 6258362 | |
| longitudinal study on rotaviruses in stool samples during the first year of life. | 1980 | 6258366 | |
| [features of gastroenteritis caused by rotavirus]. | 1980 | 6259011 | |
| rotavirus and non-bacterial infantile gastroenteritis in kuwait. | the role of rotavirus in non-bacterial gastroenteritis in kuwait was investigated. employing electronmicroscopy (em) and enzyme-linked-immuno-sorbent-assay (elisa) techniques, the virus was detected in the stools of 42 of 274 (15.3%) infants with the disease. a statistically significant association (p less than 0.05) was found between virus excretion and age since 37 of 42 (88.1%) of rotavirus positive cases were aged 2-12 months. 57.1% of all rotaviruses detected were among specimens collected ... | 1980 | 6259070 |
| human rotavirus type 2: cultivation in vitro. | a strain of type 2 human rotavirus (wa) was grown to relatively high titer through 14 passages in primary cultures of african green monkey kidney (agmk) cells. this passage series was initiated with virus that had been passaged 11 times serially in newborn gnotobiotic piglets. in contrast, virus present in the stool of patient wa as well as virus from the first, second, or third passage in piglets could not be propagated successfully in african green monkey kidney cells. prior to each passage in ... | 1980 | 6243190 |
| ultrastructural study of rotavirus replication in cultured cells. | a systematic ultrastructural analysis of the replication cycle of the simian rotavirus sa11 in permissive ma104 cells was performed under reproducible conditions. at 8 h p.i., small areas of viroplasm were seen adjacent to swollen vesicles of the rough endoplasmic reticulum (rer) containing a few 80 to 90 nm virus particles. at later times, the size and number of these inclusions increased and the rer contained large numbers of the 80 to 90 nm particles as well as 52 to 65 nm particles. infected ... | 1980 | 6243348 |
| [viruses and gastroenteritis (author's transl)]. | in recent years it has been demonstrated that a group of viruses, rotavirus for the children and norwalk agent for adults, are a highly significant cause of acute gastroenteritis during the months of winter ("winter vomiting disease"). the rotavirus was identified by bishop et al. as a double-stranded rna virus that can be isolate from faeces of children with acute gastroenteritis. viral gastroenteritis is an autolimitate disease, although under certain conditions it may even lead to severe dise ... | 1980 | 6243384 |
| acute diarrheal infections in infants i. bacterial and viral causes. | this first article of a 2-part series examined the epidemiology, therapy, and prospects for immunoprophylaxis of diarrheal infections in infants. 3 main strains of escherichia coli can be identifiied with the etiology of infantile diarrhea: enterotoxigenic e. coli, invasive e. coli, and enteropathogenic e. coli. viral agents may also be the etiological agents. specific pathogens can be identified in 55-85% of cases of infantile diarrhea today; in the 1960s, only 25-34% of cases could be ident ... | 1980 | 6243608 |
| enzyme-linked immunosorbent assays adapted for serotyping of human rotavirus strains. | five enzyme-linked immunosorbent assay systems were adapted for serotyping human rotavirus strains and were compared with a sensitive complement fixation test in terms of specificity and sensitivity. the assays differed mainly with regard to the antibody systems involved in the double sandwich. serotype differentiation of 34 rotavirus strains was achieved by determining a neutralization endpoint titer, either with a constant antiserum-varying antigen dilution method or vice versa. the procedure ... | 1980 | 6243672 |
| oral fluid replacement by a glucose glycine electrolyte formulation in e coli and rotavirus diarrhoea in pigs. | a glucose glycine electrolyte solution (gges) was investigated to determine its suitability for oral rehydration in diarrhoea in pigs. piglets with diarrhoea following experimental infection with eneteropathogenic escherichia coli were given access in cube drinkers to either gges or water. the mortality in the gges group (11.6 per cent) was significantly (p less than 0.05) less than in the controls (24.0 per cent), and weight gain in severely diarrhoeic piglets was greater in the gges group. gno ... | 1980 | 6243814 |
| stability of simian rotavirus in fresh and estuarine water. | the rates of inactivation of poliovirus 1, echovirus 7, coxsackievirus b3, and simian rotavirus sa11 were compared in polluted and nonpolluted fresh and estaurine water samples. the study was done in two parts, comparing virus survival in samples taken 1 year apart from the same sites. the survival studies were performed at 20 degrees c and at the natural ph of the water samples. in the first part of the study, the time required for a 3-log10 reduction in the initial virus titers was 2 to 3 days ... | 1980 | 6243899 |
| polyacrylamide gel electrophoresis of avian rotavirus rna. | the rnas of 4 avian rotavirus isolates were analysed by polyacrylamide gel electrophoresis. one of these isolates was made from chickens, the other three were from turkeys. the rnas were separated into 8 or 9 bands consisting of 11 segments, ranging in molecular weight from 0.24 x 10(6) to 2.09 x 10(6). in contrast to calf rotavirus, segments 10 and 11 of avian rotavirus rna had very similar or identical electrophoretic mobilities. this may be a unique feature of avian rotavirus rna. co-electrop ... | 1980 | 6243920 |
| antigenic comparisons of two new rotaviruses from rhesus monkeys. | two rotavirus strains isolated in cell culture from infant rhesus monkeys with diarrhea were closely related to sa 11 virus and to each other by plaque reduction neutralization tests. however, results of immune electron microscopy suggested possible antigenic differences between the two rhesus rotavirus strains. | 1980 | 6244332 |
| rotavirus: the first five years. | 1980 | 6244380 | |
| further studies on neonatal rotavirus infections. | forty newborn babies who were shown to be excreting rotaviruses within a few days of birth were tested daily for one month. most were found to excrete virus for only a short period of time. in 48% of babies, virus was found on one day only, in 25% it was present for two days, and in 10% for three days. most of these infections did not appear to confer lasting immunity. only 21% of young children who excreted virus as neonates had detectable circulating complement-fixing antibodies when eight to ... | 1980 | 6244482 |
| proteolytic enhancement of rotavirus infectivity: biology mechanism. | 1980 | 6244698 | |
| porcine rotaviral infection of cell culture: effects of certain enzymes. | porcine rotaviral infectivity for continuous porcine kidney (pk-15) cells was enhanced by incorporation of pancreatic endopeptidases into the cell culture maintenance medium. marked enhancement of infectivity was induced by trypsin, whereas elestase and alpha-chymotrypsin enhanced infectivity to a lesser extent. bacterial protease also induced some enhancement of porcine rotaviral infectivity. a synergistic enhancement of porcine rotaviral infectivity was noticed with trypsin and alpha-chymotryp ... | 1980 | 6244764 |
| identification of rotaviruses of different origins by the plaque-reduction test. | the plaque assay for the simian rotavirus sa11 was shown to be applicable to the economically important calf and porcine rotaviruses. in addition, neutralization of viral infectivity with specific antiserum before assay (plaque-reduction test) was found to be a useful test to identify the species of origin of rotaviruses. | 1980 | 6244765 |
| the first year of life. diarrhoea. | 1980 | 6244874 | |
| rapid diagnosis by electron microscopy of nonbacterial gastroenteritis in children. | 1980 | 6244887 | |
| gastroenteritis due to rotavirus in an isolated pacific island group: an epidemic of 3,439 cases. | in the spring of 1964, 3,439 cases of acute gastroenteritis were reported from truk district, an island group in the mid-pacific. the causative agent was not identified in laboratory studies performed in that year. retrospective serologic studies 15 years later with the complement fixation test and the enzyme-linked immunosorbent assay provided serologic evidence that this outbreak was caused by the human rotavirus that was first isolated in 1973. | 1980 | 6245146 |
| multiplication of human rotavirus in cultured cells: an electron microscopic study. | human rotaviruses were capable of efficient multiplication in llc-mk2 cells when the inoculum was pre-treated with trypsin, centrifuged on to the cell monolayer and the infected cells maintained in a medium containing trypsin. however, not all of the human rotavirus isolates used to infect cells resulted in efficient virus production. the ability of human isolates to multiply in cultured cells was studied by direct observation of virus in the electron microscope, by radioactive labelling with 3h ... | 1980 | 6245181 |
| in vitro transcription and translation of simian rotavirus sa11 gene products. | rotavirus gene products were examined, with the simian rotavirus sa11 as a model. the endogenous viral rna-dependent rna polymerase associated with single-shelled virus particles or with activated double-shelled particles was used to synthesize viral rna transcripts. sedimentation velocity sucrose gradient analysis of the rna transcripts revealed four peaks at 9s, 12s, 14s, and 18s, whereas agarose gel electrophoresis under partially denaturing conditions revealed eight groups of rna species ran ... | 1980 | 6245262 |
| coding assignments of double-stranded rna segments of sa 11 rotavirus established by in vitro translation. | the segmented double-stranded (ds) rna genome of the simian rotavirus sa 11, after denaturation, can be translated in a cell-free protein synthesizing system. of the 11 genome segments, 9 can be resolved on polyacrylamide gels and thus could be individually isolated and translated, providing a means of identifying the polypeptide encoded by each segment. on the basis of electrophoretic mobility of products in sodium dodecyl sulfate-polyacrylamide gels, the probable gene-coding assignments of dsr ... | 1980 | 6245278 |
| [role of rotavirus in gastroenteritis in infants at the university hospital center of grenoble. value of serology]. | 1980 | 6245401 | |
| diarrhea in a non-hospitalized rural salvadoran population: the role of enterotoxigenic escherichia coli and rotavirus. | to determine the role of rotavirus, enterotoxigenic escherichia coli and enteropathogenic e. coli in diarrheal disease of non-hospitalized children and adults living in rural el salvador, stool specimens were collected from 156 persons with diarrhea and 134 age- and sex-matched controls over a 1-year period. enterotoxigenic escherichia coli (etec) were isolated as frequently from controls (13.4%) as from diarrhea cases (12.2%). enteropathogenic e. coli were isolated from 13 cases (8.3%) and 10 ( ... | 1980 | 6245596 |
| cord blood and breast-milk antibodies in neonatal rotavirus infection. | studies were carried out during an outbreak of rotavirus type 2 infection in a neonatal nursery to determine the protective role of antibodies in cord blood and breast milk. the range, distribution, and geometric mean titres of rotavirus-specific antibody in the cord blood were similar among rotavirus-positive and rotavirus-negative neonates, and the amount of virus excreted did not correlate with antibody levels. despite the protective effect of breast feeding, the pattern of rotavirus-specific ... | 1980 | 6245749 |
| studies on the etiology of autumnal infantile acute gastroenteritis--rotavirus. | 1980 | 6245835 | |
| antiviral activity of colostrum and serum immunoglobulins a and g. | enteric virus-specific iga and igg present in paired human sera and colostrums were measured by the enzyme-linked immunosorbent assay (elisa). virus-specific iga was present in all colostrums, but virus-specific igg could not be detected. the reverse was true when sera were assayed. most of these colostrums also neutralized either polio virus or reovirus, as did iga, which was separated from a pool of colostrums by exclusion chromatography. no correlation could be made between levels of neutrali ... | 1980 | 6246196 |
| rotavirus and other viruses in the stool of premature babies. | in a 12-month study, 363 stools of 199 premature babies nursed in a separate ward of a paediatric clinic were examined by electron microscopy and on cell culture to detect virus. twenty-four (6.6%) were positive for rotavirus, in one winter epidemy. from four stools echo 22 was isolated, and in six cases virus-like particles were detected by electron microscopy. these virus infections are not a major problem in newborns, requiring special care, as they are mostly symptomless or mild. | 1980 | 6246197 |
| role of two particle types in bovine rotavirus morphogenesis. | the involvement of light (l) and dense (d) bovine rotavirus particle types during virus replication has been studied. it was found that infectious parental l virions are uncoated in vivo to a particle similar to native d particles. differences in the rate of synthesis and relative yields of l and d particles in mdbk and ma-104 cells have been detected. results from pulse-chase labeling experiments indicate that d particles serve as morphogenic precursors to the complete l virion. | 1980 | 6246258 |
| [rotaviruses in diarrheal diseases]. | 1980 | 6246408 | |
| viruses and diarrhoea in dogs. | 1980 | 6246671 | |
| the effect of human milk fractions on rotavirus in relation to the secretory iga content. | human milk from healthy norwegian women was fractionated by ammonium sulphate precipitation and gel filtration. the protein content, lactoferrin and secretory iga were measured. specific antirotavirus iga, detected by indirect immunofluorescence was found in one out of five milk samples before fractionation, while a more concentrated immunoglobulin fraction from the other four milk samples contained such iga. before fractionation, 3 of 5 milk samples neutralized human rota-virus infection of llc ... | 1980 | 6246713 |
| course of rotavirus gastroenteritis in a closed community. | there were 3 outbreaks of rotavirus gastroenteritis accompanied by milky-white stools in a closed community of 21 children in japan during a 2-year period. several different clinical courses were observed as a result of clinical, serological, and virological observations. | 1980 | 6246843 |
| natural epizootic diarrhea of infant mice (edim): a light and electron microscope study. | 1980 | 6247195 |