Publications

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on some genetic aspects of phage lambda resistance in e. coli k12.most mutations rendering e. coli k12 resistant to phage lambda, map in two genetic regions mala and malb.-the malb region contains a gene lamb specifically involved in the lambda receptor synthesis. twenty-one independent lamb mutations studied by complementation belonged to a single cistron. this makes it very likely that lamb is monocistronic. among the lamb mutants some are still sensitive to a host range mutant of phage lambda. mutations mapping in a proximal gene essential for maltose metab ...19724558324
maturation and recombination of bacteriophage lambda dna molecules in the absence of dna duplication. 19724559112
a reanalysis of red-mediated reciprocal recombination in bacteriophage lambda. 19724559691
mechanism of transcription in the n operon of bacteriophage lambda. 19724560678
plasmid-controlled variation in the content of methylated bases in bacteriophage lambda deoxyribonucleic acid.the n(6)-methyladenine (meade) and 5-methylcytosine (mec) contents in deoxyribonucleic acid (dna) of bacteriophage lambda has been analyzed as a function of host specificity. the following facts have emerged: (i) lambda grown on strains harboring the p1 prophage contain ca. 70 more meade residues/dna molecule than lambda grown either in the p1-sensitive parent, or in a p1 immune-defective lysogen which does not confer p1 modification; (ii) lambda grown on strains harboring the n-3 drug-resistanc ...19724561202
degradation of bacteriophage lambda deoxyribonucleic acid after restriction by escherichia coli k-12.wild-type bacteria which restrict the deoxyribonucleic acid (dna) of infecting phage when the phage do not carry the proper host modification rapidly degrade that restricted dna to acid-soluble products. the purified restriction enzyme acts as an endonuclease in vitro to cleave restrictable dna and does not further degrade the dna fragments produced. we have examined mutants of escherichia coli k-12 which lack various nucleases in order to determine which nucleases are involved in the rapid acid ...19724562392
interaction of the recombination pathways of bacteriophage lambda and host escherichia coli: effects on lambda recombination. 19724563260
interaction of the recombination pathways of bacteriophage lambda and its host escherichia coli k12: effects on exonuclease v activity. 19724563261
behavior of coliphage lambda in shigella flexneri 2a.the insensitivity of wild-type shigella flexneri 2a to coliphage lambda is a consequence of its native genetic defect in the mala gene cluster. the "smooth" s. flexneri 2a lipopolysaccharide layer affects the efficient adsorption of lambda. derivatives, capable of serving as functional hosts for lambda, were obtained by repairing the mala lesion, enabling the expression of the malb-lambdarcp region of s. flexneri. introduction of a mutation into s. flexneri causing a "rough" lipopolysaccharide c ...19724563593
formation of hybrids between coliphage lambda and salmonella phage p22 with a salmonella typhimurium hybrid sensitive to these phages.an unusual salmonella typhimurium hybrid with sensitivity to coliphage lambda and salmonella phage p22 has been recovered from matings between an escherichia coli k-12 hfr donor and an s. typhimurium recipient. the hybrid is an excellent host for achieving genetic recombination between lambda and p22. two broad classes of hybrid phages were isolated. the lambda-p22 hybrid class, which has the protein coat of lambda, contains at least the c region of p22. the p22-lambda hybrid class has the prote ...19724564201
precursor and product in bacteriophage lambda recombination.analyses of the time of recombination in bacteria infected with phage lambda and in spheroplasts infected with purified phage lambda dna indicate a preponderance of recombination occurring before replication ("early recombination") after infection with tandem dimers formed in vitro compared to linear monomer dna and phage infections. among the early recombinants is a large fraction of cells that produces one recombinant type exclusively. two suggestions are discussed-that a dimer-like molecule i ...19724564207
fine-structure deletion map of the escherichia coli l-arabinose operon.a fine-structure deletion map of the l-arabinose operon of e. coli was constructed by mapping deletion endpoints against point mutations. of 350 independent deletions with average endpoint separation of ten nucleotides, 51 ended in the control region between the c and b genes, and the rest ended in the structural genes a, b, c, and d. if deletion endpoints are randomly distributed, the c and b genes are separated by about 510 nucleotides. deletion endpoints and locations of point mutations in fa ...19724564212
methylation pattern of lambda deoxyribonucleic acid.deoxyribonucleic acid (dna) extracted from phage lambda grown on escherichia coli k-12 strain w4032 had 113 +/- 10 5-methylcytosine residues and 215 +/- 20 6-methyl adenine residues per genome, as determined by three independent methods. these methylated nucleotides were distributed equally among the two strands of lambda dna. shearing of double-stranded dna to half-length fragments revealed a slight deficiency of 5-methyl cytosine in the 55% guanine plus cytosine half. shearing the dna to fragm ...19724564587
purification and properties of d protein: a transcription factor of escherichia coli.d factor is protein that stimulates dna transcription in vitro by rna polymerase. for dna from bacteriophage lambda, we present evidence that d protein interacts with dna to increase the frequency and improve the specificity of initiation by rna polymerase.19724566455
gene expression by constitutive mutants of coliphage lambda. 19725015426
isolation and structure of phage lambda head-mutant dna. 19725022188
head assembly steps controlled by genes f and w in bacteriophage lambda. 19725022189
tandem genetic duplications in a derivative of phage lambda. ii. evidence for structure and location of endpoints. 19725023665
relationship between the n function of bacteriophage lambda and host rna polymerase. 19725040359
chemical synthesis of the "sticky end" of lambda phage dna r-strand. 19725043189
repression by the ci protein of phage lambda: in vitro inhibition of rna synthesis. 19725045305
repression by the ci protein of phage lambda: interaction with rna polymerase. 19725045306
lambda phage mutants insensitive to temperature-sensitive repressor. 3. effects of virl, virr or virc mutation on the gene expression. 19725046962
isolation and characterization of deletions in bacteriophage lambda residing as prophage in e. coli k 12. 19725057547
dna-replication of spi - mutants of bacteriophage lambda in recombination-deficient bacteria. 19725057549
the net hydration of phage lambda. 19725078393
new banding species, with densities of phage-ghost clusters, produced from lambda phage particles on storage. 19725078394
isolation and properties of rex - mutants of bacteriophage lambda.twenty-five rex(-) mutants of phage lambda have been isolated. three of the mutants, including one amber mutant, map at three distinct sites within the rex region of the lambda genetic map. the existence of the amber mutant provides further evidence that rex and ci are distinct genes, since it seems to be identical to wild-type lambda in its ability to establish or maintain lysogeny.19725084465
polar mutations in lac, gal and phage lambda consist of a few is-dna sequences inserted with either orientation. 19724567156
increased stability and reproducibility of escherichia coli spheroplasts in the transfection assay of phage lambda dna with polyethylene glycol instead of sucrose. 19724622293
pleiotropic properties and genetic organization of the tola,b locus of escherichia coli k-12.colicin-tolerant mutants of escherichia coli k-12, which map near gal at 17 min (tola, b mutants), have been isolated and characterized. these mutants exhibited a very broad spectrum of phenotypic changes consistent with the interpretation that they are cell surface mutants. in addition to being colicintolerant and sensitive to deoxycholate and ethylenediaminetetraacetic acid, tola, b mutants are sensitive to vancomycin, bacitracin, and dodecyl sulfate. the tola, b mutants from most strains also ...19724627928
influence of proflavine on the cyclization and aggregation of phage lambda dna. 19724660954
factors influencing competence of escherichia coli for lambda-phage deoxyribonucleic acid infection. 19724569211
host specificity of salmonella typhimurium deoxyribonucleic acid restriction and modification.the restriction and modification genes of salmonella typhimurium which lie near the thr locus were transferred to a restrictionless mutant of escherichia coli. these genes were found to be allelic to the e. coli k, b, and a restriction and modification genes. e. coli recombinants with the restriction and modification host specificity of s. typhimurium restricted phage lambda that had been modified by each of the seven known host specificities of e. coli at efficiency of plating levels of about 1 ...19734570605
bacterial cell division regulation: lysogenization of conditional cell division lon - mutants of escherichia coli by bacteriophage.the lon(-) mutants of escherichia coli grow apparently normally except that, after temporary periods of inhibition of deoxyribonucleic acid synthesis, septum formation is specifically inhibited. under these conditions, long, multinucleate, nonseptate filaments result. the lon(-) mutation also creates a defect such that wild-type bacteriophage lambda fails to lysogenize lon(-) mutants efficiently and consequently forms clear plaques on a lon(-) host. two lines of evidence suggest that this failur ...19734570782
characterization of the mrna transcribed from the head and tail genes of phage lambda chromosome. 19734573271
lysogenization by bacteriophage lambda. i. multiple infection and the lysogenic response. 19734573866
e. coli mutants produced by the insertion of bacteriophage lambda dna. 19734576121
genetic recombination induced by dna cross-links in repressed phage lambda. 19734576122
excretion of enterochelin by exba and exbb mutants of escherichia coli.escherichia coli mutants that are insensitive to colicins b and i hyperproduce and excrete the iron chelator enterochelin, which is an inhibitor of these colicins. these mutants are classified as exba and exbb. the exba mutants are chromium sensitive and require iron for growth, and the mutations are located in the tonb region at min 25 of the e. coli chromosome. tonb mutants in which the genome of phage lambda is inserted into the bacterial chromosome within the tonb gene also exhibit enteroche ...19734576403
comparison of the resa1 and pola1 mutations in isogenic strains of escherichia coli k-12.strains carrying either the pola1 or resa1 mutation are deficient in dna polymerase i, and the pola1 and resa1 mutations do not complement in merozygotes. the effect of these mutations in otherwise identical genetic backgrounds was studied: after ultraviolet irradiation both strains degrade their dna more rapidly and more extensively than the wild-type strains. however, after x-ray irradiation the resa1 strain shows little dna breakdown and repairs its single-strand breaks. in contrast, the pola ...19734576410
prophage induction by high temperature in thermosensitive dna mutants lysogenic for bacteriophage lambda.high-temperature treatment of thermosensitive dna mutants lysogenic for phage lambda leads to prophage induction and release of phage (at the permissive temperature) in elongation-defective mutants of the genotypes dnab, dnae, and dnag. in initiation-defective mutants no prophage induction occurs at 42 c in mutants of the genotype dnaa, whereas with a dnac mutant as well as with strain hfrh 252 (map position not yet known) phages are released at 42 c. dna degradation at the replication fork at 4 ...19734576518
[bacteriophage lambda dna-dependent synthesis of endolysin in a membranous cell-free system of e. coli]. 19734576624
intermediates in the maturation of bacteriophage lambda dna. 19734576745
a sedimentation analysis of dna found in escherichia coli infected with phage lambda mutants. 19734576746
replicator mutants of bacteriophage lambda: characterization of two subclasses. 19734576747
bacteriophage lambda dna synthesized by mutants in gene n. 19734576748
ultraviolet reactivation of lambda phage: assay of infectivity of dna molecules by spheroplast transfection. 19734577040
properties of a mutant of escherichia coli defective in bacteriophage lambda head formation (groe). i. initial characterization. 19734578098
properties of a mutant of escherichia coli defective in bacteriophage lambda head formation (groe). ii. the propagation of phage lambda. 19734578099
host participation in bacteriophage lambda head assembly. 19734578100
isolation of a ribonuclease associated with the formation of phage lambda-ci. 19734578800
structural change of dna during lambda-phage lysogenization and induction. 19734579423
role of dna topology in transcription of coliphage lambda in vivo. ii. dna topology protects the template from exonuclease attack. 19734579547
incisions in ultraviolet irradiated circular bacteriophage lambda dna molecules in excision proficient and deficient lysogens of e. coli. 19734579985
the role of c3 product and anti-immunity in zygotic induction of bacteriophage lambda. 19734580270
replication of bacteriophage lambda dna dependent on the function of host and viral genes. i. interaction of red, gam and rec. 19734580674
an analysis of the processes of infection and induction of e. coli mutant hfl-1 by bacteriophage lambda. 19734580731
postirradiation fate of bacteriophage lambda dna in the host cell. 19734580735
physical studies of rna involvement in bacteriophage lambda dna replication and prophage excision. 19734580911
requirement for transcription in the neighborhood of the phage attachment region for lysogenization of escherichia coli by bacteriophage lambda. 19734580912
distinct effects of diamines, polyamines, and magnesium ions on the stability of lambda phage heads. 19734581784
repair of gamma-ray-induced damage in bacteriophage lambda. 19734582608
influence of thymine starvation on the integrity of episomal and chromosomal deoxyribonucleic acids in escherichia coli cr34 (lambda ind-).the effect of thymine deprivation on the integrity of phage lambda, sex factor, and chromosomal deoxyribonucleic acid (dna) in escherichia coli cr34 (lambda ind(-)) was examined by sedimenting cell lysates through alkaline sucrose gradients. both sex factor and chromosomal dnas showed evidence of being degraded during the starvation period. in contrast, no loss of closed circular lambda dna was observed.19734583234
a new effect of the rex gene of phage lambda: premature lysis after infection by phage t1. 19734583305
genetic characterization of a prm- mutant of bacteriophage lambda. 19734583306
loss of an essential function of escherichia coli by deletions in the thya region.in an attempt to obtain deletions in the thya gene, an abnormal lysogen of lambda having the prophage inserted between the thya and lysa genes was induced, and the surviving cured cells were examined for thy(-) and lys(-) mutants. in nearly 10,000 cured cells, 184 lys(-) but no thy(-) mutants were found. at the same time, the induced lambda phage contained an approximately equivalent number of lambdathya(+) and lambdalysa(+) transducing particles. by contrast, in a strain with the genotype f' th ...19734584799
infection of escherichia coli envelope-membrane complex with lambda phage: adsorption and penetration. 19734585981
fate of bacteriophage lambda in non-immune germ-free mice. 19734586796
role of the cro gene in bacteriophage lambda development. 19734587402
prophage lambda at unusual chromosomal locations. ii. mutations induced by bacteriophage lambda in escherichia coli k12. 19734587404
regulation of bacteriophage lambda development. 19734593172
control of gene expression in bacteriophage lambda. 19734593306
evidence that pretreatment of escherichia coli cells with n-methyl-n'-nitro-n-nitrosoguanidine enhances mutability of subsequently infecting phage lambda. 19734593995
on maturation of the bacteriophage lambda chromosome. 19734594001
[study of the characteristics of lambda phage dna transfection in e. coli cells treated with cacl2. the role of ca2+ ions at various stages of phage transfection]. 19734594845
expression of the trp operon in phi80trp transducing phages. orientation of transcription and an artificial high-efficiency promotor in phage lambda h +phi80 pt5-2ab. 19734686211
two repressors in bacteriophage lambda. 19734686213
repair mechanisms involved in prophage reactivation and uv reactivation of uv-irradiated phage lambda. 19734688367
lack of specific inhibition of vaccinia plaque formation by bacteriophage lambda products.unsuccessful efforts to confirm the production of a specific antiviral agent (phagicin) by bacteriophage lambda are reported.19734716545
repressor and rex product of coliphage lambda: lack of collaboration and joint controls. 19734725965
effect of the n-bypass mutations nin and byp on the rightward transcription in coliphage lambda. 19734746875
nucleotide sequence analysis of dna. xi. the 3' terminal sequences of bacteriophage lambda and phi 80 dna. 19734753202
a minor pathway leading to plaque-forming particles in bacteriophage lambda. studies on the function of gene d. 19734758067
mutations in the right operator of bacteriophage lambda: evidence for operator-promoter interpenetration. 19734758068
mutations in the right operator of bacteriophage lambda: physiological effects. 19734758069
characterization of polypeptides made in vitro from bacteriophage lambda dna. 19734762922
restriction of bacteriophage lambda by escherichia coli k. 19734767461
deletion mutants of bacteriophage lambda. iv. high frequency int-promoted recombination. 19734772128
attachment site mutants of bacteriophage lambda. 19734778806
gene n regulator function of phage lambda immun21: evidence that a site of n action differs from a site of n recognition. 19734778807
control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda. 19734784811
positive and negative control of transcription in bacteriophage lambda. 19734807323
isolation of a new rna polymerase-binding protein from sporulating bacillus subtilis.rna polymerase was precipitated from extracts of radioactively labeled vegetative and sporulating bacillus subtilis with antiserum prepared against vegetative core polymerase. the precipitates were solubilized and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum added to an extract of vegetative b. subtilis precipitated only the known subunits of core rna polymerase, but antiserum added to an extract of sporulating cells precipitated a new polypeptide of 70,000 da ...19734631355
repressor, operators, and promoters in bacteriophage lambda. 19734618262
transfection of escherichia coli spheroplasts. ii. relative infectivity of native, denatured, and renatured lambda, t7, t5, t4, and p22 bacteriophage dnas.the change of infectivity of phage dnas after heat and alkali denaturation (and renaturation) was measured. t7 phage dna infectivity increased 4- to 20-fold after denaturation and decreased to the native level after renaturation. both the heavy and the light single strand of t7 phage dna were about five times as infective as native t7 dna. t4 and p22 phage dna infectivity increased 4- to 20-fold after denaturation and increased another 10- to 20-fold after renaturation. these data, combined with ...19734591046
genetic analysis of bacteriophage lambda strains which transduce the genes for leucine biosynthesis. 19734591366
repair mechanisms involved in the recovery of phage lambda from ultraviolet damage. 19734618990
interference with the expression of the n gene function of phage lambda in a mutant of escherichia coli. 19734567743
an endonuclease induced by bacteriophage lambda. 19734567785
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