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dengue vaccines: recent developments, ongoing challenges and current candidates.dengue is among the most prevalent and important arbovirus diseases of humans. to effectively control this rapidly spreading disease, control of the vector mosquito and a safe and efficacious vaccine are critical. despite considerable efforts, the development of a successful vaccine has remained elusive. multiple factors have complicated the creation of a successful vaccine, not the least of which are the complex, immune-mediated responses against four antigenically distinct serotypes necessitat ...023984962
aedes taeniorhynchus vectorial capacity informs a pre-emptive assessment of west nile virus establishment in galápagos.increased connectivity with the mainland has led to the arrival of many invasive species to the galápagos islands, including novel pathogens, threatening the archipelago's unique fauna. here we consider the potential role of the mosquito aedes taeniorhynchus in maintaining the flavivirus west nile virus [wnv] should it reach the islands. we report on three components of vectorial capacity - vector competency, distributional abundance and host-feeding. in contrast to usa strains, galápagos a. tae ...023519190
urban mosquito fauna in mérida city, méxico: immatures collected from containers and storm-water drains/catch basins.we examined the species composition and temporal occurrence of immature mosquitoes in containers and storm-water drains/catch basins from november 2011 to june 2013 in mérida city, méxico. a wide range of urban settings were examined, including residential premises, vacant lots, parking lots, and streets or sidewalks with storm-water drains/catch basins. in total, 111,776 specimens of 15 species were recorded. the most commonly collected species were aedes (stegomyia) aegypti (l.) (n = 60,961) a ...025429168
dengue: challenges for policy makers and vaccine developers.because of the increasing incidence, geographic expansion and economic burden of dengue transmission, dengue poses major challenges to policy makers. a vaccine against dengue is urgently needed, but vaccine development has been hampered by the lack of an appropriate animal model, poor understanding of correlates of successful human immunity, the fear of immune enhancement, and viral interference in tetravalent combinations. the most suitable target epitopes for vaccines, as well as the role of n ...024781826
molecular detection and typing of dengue viruses from archived tissues of fatal cases by rt-pcr and sequencing: diagnostic and epidemiologic implications.diagnosis of dengue virus (denv) infection in fatal cases is challenging because of the frequent unavailability of blood or fresh tissues. for formalin-fixed, paraffin-embedded (ffpe) tissues immunohistochemistry (ihc) can be used; however, it may not be as sensitive and serotyping is not possible. the application of reverse transcription-polymerase chain reaction (rt-pcr) for the detection of denv in ffpe tissues has been very limited. we evaluated ffpe autopsy tissues of 122 patients with susp ...022302871
deadly intracranial bleed in patients with dengue fever: a series of nine patients and review of literature.dengue fever is a global pandemic threat with increasing incidence. to date, there are no cures and the effectiveness of dengue vaccines is still uncertain. world heath organization introduced expanded dengue syndrome to include unusual presentations of dengue fever including severe neurologic complications. one of the deadly complications is intracranial hemorrhage (ich).027365962
molecular epidemiology and phylogenetic analysis of dengue virus type-1 and 2 isolated in malaysia.detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus.026150855
re-emergence of dengue virus serotype 2 strains in the 2013 outbreak in nepal.epidemiological interventions and mosquito control are the available measures for dengue control. the former approach uses serotype and genetic information on the circulating virus strains. dengue has been frequently reported from nepal, but this information is mostly lacking. the present study was done to generate a comprehensive clinical and virological picture of a dengue outbreak in nepal during 2013.026905233
reemergence and autochthonous transmission of dengue virus, eastern china, 2014.in 2014, 20 dengue cases were reported in the cities of wenzhou (5 cases) and wuhan (15 cases), china, where dengue has rarely been reported. dengue virus 1 was detected in 4 patients. although most of these cases were likely imported, epidemiologic analysis provided evidence for autochthonous transmission.026292098
co-circulation of dengue and chikungunya viruses, al hudaydah, yemen, 2012.we investigated 400 cases of dengue-like illness in persons hospitalized during an outbreak in al hudaydah, yemen, in 2012. overall, 116 dengue and 49 chikungunya cases were diagnosed. dengue virus type 2 was the predominant serotype. the co-circulation of these viruses indicates that mosquitoborne infections represent a public health threat in yemen.025061762
reply to skalsky et al.: a microrna-like small rna from dengue virus. 025061641
vertical transmission of dengue virus in aedes aegypti collected in puerto iguazú, misiones, argentina.a finding of vertical transmission of the den 3 virus in male specimens of aedes aegypti, collected in the 2009 fall-winter period, in puerto iguazú city, misiones, argentina, using the rt-pcr technique in a 15-specimen pool is reported. this result is analyzed within the context of the epidemiological situation of argentina's northeast border.024626420
phylogeography of dengue virus serotype 4, brazil, 2010-2011.dengue virus serotype 4 (denv-4) reemerged in roraima state, brazil, 28 years after it was last detected in the country in 1982. to study the origin and evolution of this reemergence, full-length sequences were obtained for 16 denv-4 isolates from northern (roraima, amazonas, pará states) and northeastern (bahia state) brazil during the 2010 and 2011 dengue virus seasons and for an isolate from the 1982 epidemic in roraima. spatiotemporal dynamics of denv-4 introductions in brazil were applied t ...023092706
mosquito larvicidal properties of mirabilis jalapa (nyctaginaceae) against anopheles stephensi, aedes aegypti & culex quinquefasciatus (diptera: culicidae). 025366214
updating the geographical distribution and frequency of aedes albopictus in brazil with remarks regarding its range in the americas.the geographical distribution of aedes albopictus in brazil was updated according to the data recorded across the country over the last eight years. countrywide house indexes (hi) for ae. albopictus in urban and suburban areas were described for the first time using a sample of brazilian municipalities. this mosquito is currently present in at least 59% of the brazilian municipalities and in 24 of the 27 federal units (i.e., 26 states and the federal district). in 34 brazilian municipalities, th ...025317707
imported dengue fever in tuscany, italy, in the period 2006 to 2012.this report focuses on epidemiological and clinical features of dengue fever (df) in tuscany (italy) between 2006 and 2012. sixty-one df cases were diagnosed, 32 of which were in the period of aedes albopictus activity. some clinical (arthralgia/myalgia, nausea/vomiting, and skin rash), laboratory (leukopenia and thrombocytopenia), and epidemiological characteristics (travel in a continent other than africa) significantly distinguished df cases from other febrile illnesses. our data stress the i ...025155927
small bite, big threat: the burden of vector-borne diseases. 025909072
clinical applicability of various dengue diagnostic tests in resource-limited endemic settings.dengue is one of the most important arboviral infections caused by one of the four dengue serotypes, 1-4.025191051
zika virus: indian perspectives.the emergence of zika virus (ziv), a mosquito borne flavivirus like dengue (den) and chikungunya (chik), in brazil in 2014 and its spread to various countries have led to a global health emergency. aedes aegypti is the major vector for ziv. fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ziv and there is abundance of aedes mosquitoes. in this review, we focus on current global scenar ...027487998
zika virus infection after travel to tahiti, december 2013. 025062427
evidence that implicit assumptions of 'no evolution' of disease vectors in changing environments can be violated on a rapid timescale.projected impacts of climate change on vector-borne disease dynamics must consider many variables relevant to hosts, vectors and pathogens, including how altered environmental characteristics might affect the spatial distributions of vector species. however, many predictive models for vector distributions consider their habitat requirements to be fixed over relevant time-scales, when they may actually be capable of rapid evolutionary change and even adaptation. we examine the genetic signature o ...025688024
rodents and risk in the mekong delta of vietnam: seroprevalence of selected zoonotic viruses in rodents and humans.in the mekong delta in southern vietnam, rats are commonly traded in wet markets and sold live for food consumption. we investigated seroprevalence to selected groups of rodent-borne viruses among human populations with high levels of animal exposure and among co-located rodent populations. the indirect fluorescence antibody test (ifat) was used to determine seropositivity to representative reference strains of hantaviruses (dobrava virus [dobv], seoul virus [seov]), cowpox virus, arenaviruses ( ...025629782
travel medicine for the extreme traveler. 017101310
the roles of mosquito and bird communities on the prevalence of west nile virus in urban wetland and residential habitats.this study investigated the impacts of urban wetlands and their adjacent residential environments on the transmission dynamics of west nile virus (wnv) within the state of new jersey (usa). a working hypothesis was that urban wetlands decrease the local prevalence of wnv through the dilution effect from increased bird diversity, and through relative reductions in the numbers of competent avian host and mosquito species commonly associated with wnv. surveys of mosquito and bird communities were u ...025484570
the burden of dengue and chikungunya worldwide: implications for the southern united states and california.dengue virus (denv) spreads to humans through the bite of an infected aedes aegypti or aedes albopictus mosquito and is a growing public health threat to both industrialized and developing nations worldwide. outbreaks of autochthonous dengue in the united states occurred extensively in the past but over the past 3 decades have again taken place in florida, hawaii, and texas as well as in american samoa, guam, northern mariana islands, puerto rico, and the us virgin islands. as the aedes vectors ...025960096
larvicidal & ovicidal efficacy of pithecellobium dulce (roxb.) benth. (fabaceae) against anopheles stephensi liston & aedes aegypti linn. (diptera: culicidae).in view of the recently increased interest in developing plant origin insecticides as an alternative to chemical insecticide, this study was undertaken to assess the larvicidal and ovicidal potential of the crude hexane, benzene, chloroform, ethyl acetate and methanol solvent extracts from the medicinal plant pithecellobium dulce against the mosquito vectors, anopheles stephensi and aedes aegypti (diptera: culicidae).024056567
preliminary findings on bagaza virus (flavivirus: flaviviridae) growth kinetics, transmission potential & transovarial transmission in three species of mosquitoes.bagaza virus (bagv), a flavivirus synonymous with israel turkey meningoencephalitis virus, has been found to circulate in india. bagv has recently been held responsible for inducing febrile illness in humans and causing unusually high mortality to wild birds in spain. a study was therefore, undertaken to determine its replication kinetics in certain mosquitoes and to determine vector competence and potential of the mosquitoes to transmit bagv experimentally.024056604
interspecific larval competition between aedes albopictus and aedes japonicus (diptera: culicidae) in northern virginia.aedes albopictus (skuse) and aedes japonicus (theobald) are two of the most recent and widespread invasive mosquito species to have become established in the united states. the two species co-occur in water-filled artificial containers, where crowding and limiting resources are likely to promote inter- or intraspecific larval competition. the performance of northern virginia populations of ae. japonicus and ae. albopictus competing as larvae under field conditions was evaluated. per capita rates ...018714861
neurological infections in the returning international traveler.clinicians may encounter international travelers returning with exotic infections, emerging infectious diseases, or resurgent old-world infections. many of these infectious diseases can affect the nervous system directly or indirectly. the contemporary neurologist should therefore be cognizant of the clinical manifestations, potential complications, and appropriate management of common travel-related infections. this chapter focuses on five important infections that affect the central nervous sy ...025147444
dengue antibody prevalence in german travelers.we studied 2,259 german citizens after they returned from dengue-endemic countries from 1996 to 2004. serotype-specific dengue antibodies indicated acute infections in 51 (4.7%) travelers with recent fever and 13 (1.1%) travelers with no recent fever, depending largely on destination and epidemic activity in the countries visited.015890135
the tortoise or the hare? impacts of within-host dynamics on transmission success of arthropod-borne viruses.arthropod-borne viruses (arboviruses) are maintained in a cycle of alternating transmission between vertebrate hosts and arthropod vectors. arboviruses possess rna genomes capable of rapid diversification and adaptation, and the between-host trade-offs inherent to host alternation impose well-documented constraints on arbovirus evolution. here, we investigate the less well-studied within-host trade-offs that shape arbovirus replication dynamics and transmission. arboviruses generally establish l ...026150665
assessment of arbovirus surveillance 13 years after introduction of west nile virus, united states.before 1999, the united states had no appropriated funding for arboviral surveillance, and many states conducted no such surveillance. after emergence of west nile virus (wnv), federal funding was distributed to state and selected local health departments to build wnv surveillance systems. the council of state and territorial epidemiologists conducted assessments of surveillance capacity of resulting systems in 2004 and in 2012; the assessment in 2012 was conducted after a 61% decrease in federa ...026079471
outbreak of dengue virus serotype-2 (denv-2) of cambodian origin in manipur, india - association with meteorological factors.dengue is a major health problem in many parts of india and its neighbouring countries. dengue cases have not been reported from manipur, a northeastern state of india till 2007. but, the sudden outbreak of fever with febrile illness during 2007 and 2008, suspected to be dengue/dengue haemorrhagic fever was investigated to detect the causative agent. potential impact of climatic variables on dengue transmission has been documented and hence the association between climatic factors, entomological ...023168706
the new health threats of exotic and global travel. 023265199
economic value of dengue vaccine in thailand.with several candidate dengue vaccines under development, this is an important time to help stakeholders (e.g., policy makers, scientists, clinicians, and manufacturers) better understand the potential economic value (cost-effectiveness) of a dengue vaccine, especially while vaccine characteristics and strategies might be readily altered. we developed a decision analytic markov simulation model to evaluate the potential health and economic value of administering a dengue vaccine to an individual ...021540387
serratia odorifera mediated enhancement in susceptibility of aedes aegypti for chikungunya virus.the susceptibility of the mosquito to the invading pathogen is predominantly dictated by the complex interactions between the mosquito midgut and the surface proteins of the invading pathogen. it is well documented that the midgut microbiota plays an important role in determining the susceptibility of the mosquito to the pathogen. in the present study, we investigated the influence of serratia odorifera, an endogenous cultivable midgut inhabitant of aedes aegypti on the chikungunya virus (chikv) ...025027087
larvicidal efficacy of ethiopian ethnomedicinal plant juniperus procera essential oil against afrotropical malaria vector anopheles arabiensis (diptera: culicidae).to screen the essential oil of juniperus procera (j. procera) (cupressaceae) for larvicidal activity against late third instar larvae of anopheles arabiensis (an. arabiensis) patton, the principle malaria vector in ethiopia.025183156
health economics of dengue: a systematic literature review and expert panel's assessment.dengue vaccines are currently in development and policymakers need appropriate economic studies to determine their potential financial and public health impact. we searched five databases (pubmed, embase, lilac, econlit, and wholis) to identify health economics studies of dengue. forty-three manuscripts were identified that provided primary data: 32 report economic burden of dengue and nine are comparative economic analyses assessing various interventions. the remaining two were a willingness-to ...021363989
regulation of cell survival and death during flavivirus infections.flaviviruses, ss(+) rna viruses, include many of mankind's most important pathogens. their pathogenicity derives from their ability to infect many types of cells including neurons, to replicate, and eventually to kill the cells. flaviviruses can activate tumor necrosis factor α and both intrinsic (bax-mediated) and extrinsic pathways to apoptosis. thus they can use many approaches for activating these pathways. infection can lead to necrosis if viral load is extremely high or to other types of c ...024921001
dengue virus infection: current concepts in immune mechanisms and lessons from murine models.dengue viruses (denv), a group of four serologically distinct but related flaviviruses, are responsible for one of the most important emerging viral diseases. this mosquito-borne disease has a great impact in tropical and subtropical areas of the world in terms of illness, mortality and economic costs, mainly due to the lack of approved vaccine or antiviral drugs. infections with one of the four serotypes of denv (denv-1-4) result in symptoms ranging from an acute, self-limiting febrile illness, ...024182427
knowledge and use of personal protective measures against mosquito borne diseases in a resettlement colony of delhi.mosquito borne diseases (mbds) are major public health problem in india. state of delhi is endemic for dengue and other mbds. the increasing incidence of mbds in delhi in recent years warrants a pro-active approach for their prevention. knowledge and use of personal protective measures (ppms) presents an effective strategy for prevention and control of mbds.024761243
mass scale screening of common arboviral infections by an affordable, cost effective rt-pcr method.to develop a rapid, cost effective rt-pcr method for the mass scale diagnosis of such diseases at the viremia stage to find out the actual disease burden in that area.023569876
unsuspected dengue and acute febrile illness in rural and semi-urban southern sri lanka.dengue virus (denv), a globally emerging cause of undifferentiated fever, has been documented in the heavily urbanized western coast of sri lanka since the 1960s. new areas of sri lanka are now being affected, and the reported number and severity of cases have increased. to study emerging denv in southern sri lanka, we obtained epidemiologic and clinical data and acute- and convalescent-phase serum samples from patients >2 years old with febrile illness. we tested paired serum samples for denv i ...022304972
mosquito biting activity on humans & detection of plasmodium falciparum infection in anopheles stephensi in goa, india.knowledge of the bionomics of mosquitoes, especially of disease vectors, is essential to plan appropriate vector avoidance and control strategies. information on biting activity of vectors during the night hours in different seasons is important for choosing personal protection measures. this study was carried out to find out the composition of mosquito fauna biting on humans and seasonal biting trends in goa, india.022382193
a cluster of dengue cases in american missionaries returning from haiti, 2010.dengue is an acute febrile illness caused by four mosquito-borne dengue viruses (denv-1 to -4) that are endemic throughout the tropics. after returning from a 1-week missionary trip to haiti in october of 2010, 5 of 28 (18%) travelers were hospitalized for dengue-like illness. all travelers were invited to submit serum specimens and complete questionnaires on pre-travel preparations, mosquito avoidance practices, and activities during travel. denv infection was confirmed in seven (25%) travelers ...022232444
zika virus: a roar after years of whispering. 027168917
the emergence of zika virus as a global health security threat: a review and a consensus statement of the indusem joint working group (jwg).the zika virus (zikv), first discovered in 1947, has emerged as a global public health threat over the last decade, with the accelerated geographic spread of the virus noted during the last 5 years. the world health organization (who) predicts that millions of cases of zikv are likely to occur in the americas during the next 12 months. these projections, in conjunction with suspected zika-associated increase in newborn microcephaly cases, prompted who to declare public health emergency of intern ...027013839
novel and viable acetylcholinesterase target site for developing effective and environmentally safe insecticides.insect pests are responsible for human suffering and financial losses worldwide. new and environmentally safe insecticides are urgently needed to cope with these serious problems. resistance to current insecticides has resulted in a resurgence of insect pests, and growing concerns about insecticide toxicity to humans discourage the use of insecticides for pest control. the small market for insecticides has hampered insecticide development; however, advances in genomics and structural genomics of ...022280344
a search for mosquito larvicidal compounds by blocking the sterol carrying protein, aescp-2, through computational screening and docking strategies.sterol is a very vital compound for most of the insects and mosquitoes to complete their life cycle. unfortunately mosquitoes cannot synthesize the sterol, it depends on mammals for the same. mosquitoes take the sterol from the plant decays during their larval stage in the form of phytosterol, which is then converted to cholesterol for further growth and reproduction. this conversion occurs with the help of the sterol carrier protein 2(scp2).021808576
neurological manifestations of zika virus infection.zika virus (zikv) is a flavivirus (flaviviridae family) transmitted mainly by aedes mosquitoes. the virus was restricted to the african continent until its spread to south-east asia in the 1980's, the micronesia in 2007, the french polynesia in 2013 and, more recently in the americas in 2015, where, up to date, the world health organization (who) has estimated about 3-4 million total cases of zikv infection. during outbreaks in the french polynesia and brazil in 2013 and 2015, respectively, nati ...027878100
zika in america: the year in review.short on funds and with no therapeutics or vaccines in sight, u.s. health officials are scrambling to prepare for a protracted fight with zika virus and the mosquitoes that carry it. in this article, the author focuses on the arrival of zika in the u.s.027990081
dengue fever in travelers to the tropics, 1998 and 1999.dengue fever (df) has become common in western travelers to the tropics. to improve the basis for travel advice, risk factors and dengue manifestations were assessed in 107 swedish patients for whom df was diagnosed after return from travel in 1998 and 1999. patient data were compared with data on a sample of all swedish travelers to dengue-endemic countries in the same years. only three of the patients had received pretravel advice concerning df from their physicians. hemorrhagic manifestations ...012702223
classification of dengue illness based on readily available laboratory data.the aim of this study was to examine retrospective dengue-illness classification using only clinical laboratory data, without relying on x-ray, ultrasound, or percent hemoconcentration. we analyzed data from a study of children who presented with acute febrile illness to two hospitals in thailand. multivariable logistic regression models were used to distinguish: (1) dengue hemorrhagic fever (dhf) versus dengue fever (df), (2) dhf versus df + other febrile illness (ofi), (3) dengue versus ofi, a ...020889865
zika virus: epidemiology, current phobia and preparedness for upcoming mass gatherings, with examples from world olympics and pilgrimage.to describe zika virus (zikv) epidemiology, current phobia, and the required preparedness for its prevention during the upcoming mass gathering (mg) events.027648063
mayaro virus and dengue virus 1 and 4 natural infection in culicids from cuiabá, state of mato grosso, brazil.this study aimed to verify the diversity of culicidae species and their frequency of infection with flaviviruses and alphaviruses in cuiabá, state of mato grosso, brazil. mosquitoes were captured with nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (rt-pcr) for 11 flavivirus and five alphavirus. p ...026784852
is transfusion-transmitted dengue fever a potential public health threat?dengue is an arboviruses due to single-stranded enveloped ribonucleic acid viruses, named dengue viruses (denv), that include four serotypes and are mainly transmitted via the bite of mosquitoes of the genus aedes (a. aegypti and a. albopictus). the distribution of the disease was historically limited to intertropical areas; however, during the last thirty years, the perimeter of the disease extended considerably and temperate areas are now at risk of outbreaks. the present global burden of deng ...025964876
indigenous dengue fever, buenos aires, argentina. 018760035
advances and controversies in yellow fever vaccination.ever since its development in 1937, the live-attenuated 17d yellow fever (yf) vaccine has been one of the most effective vaccines available to man. in this review we highlight the major steps in the development of 17d yf vaccine. we discuss the use of neutralizing antibodies as a surrogate marker for protection, and explore the strengths and weaknesses of the current plaque reduction neutralization test (prnt), a technique developed in the 1960s that continues to be superior to every modern test ...024757521
geographic variation in adult survival and reproductive tactics of the mosquito aedes albopictus.climate differences across latitude can result in seasonal constraints and selection on life history characters. since aedes albopictus (skuse) invaded north america in the mid-1980s, it has spread across a range of approximately 14 degrees latitude and populations in the north experience complete adult mortality due to cold winter temperatures that are absent in the south. life table experiments were conducted to test for differences in the adult survival and reproductive schedules of ae. albop ...018402136
emerging viral diseases. 018491811
thoughts around the zika virus crisis.as a lot of reviews are available on zika virus (zikv), in this short commentary, we will focus on the recent advances and gaps in knowledge regarding our understanding of zikv infections and on the reaction to the "zikv crisis."027832453
the mysterious zika virus: adding to the tropical flavivirus mayhem.until now, known as the demure cousin of dengue virus (denv) inhabiting africa, zika virus (zikv) has reinvented itself to cause explosive epidemics captivating the western hemisphere. the outbreak causing potential for zikv was realized when it made its way from africa to yap island micronesia in 2007, and in french polynesia in 2013. from there, it moved on to brazil in 2015. now zikv has infected people in more than 33 countries in central and south america and the caribbean. moreover the epi ...027763483
vector-borne disease, cities, and climate change. 027737979
culex mosquitoes are experimentally unable to transmit zika virus.we report that two laboratory colonies of culex quinquefasciatus and culex pipiens mosquitoes were experimentally unable to transmit zikv either up to 21 days post an infectious blood meal or up to 14 days post intrathoracic inoculation. infectious viral particles were detected in bodies, heads or saliva by a plaque forming unit assay on vero cells. we therefore consider it unlikely that culex mosquitoes are involved in the rapid spread of zikv.027605159
experimental investigation of the susceptibility of italian culex pipiens mosquitoes to zika virus infection.we investigated the susceptibility of an italian population of culex pipiens mosquitoes to zika virus (zikv) infection, tested in parallel with aedes aegypti, as a positive control. we analysed mosquitoes at 0, 3, 7, 10, 14, 20 and 24 days after an infectious blood meal. viral rna was detected in the body of cx. pipiens up to three days post-infection, but not at later time points. our results indicate that cx. pipiens is not susceptible to zikv infection.027605056
zika in the united states: how are we preparing? 027581255
contribution of dengue fever to the burden of acute febrile illnesses in papua new guinea: an age-specific prospective study.malaria is a major contributor to the burden of febrile illnesses in papua new guinea (png). dengue fever (df) is likely to contribute; however, its epidemiology in png is poorly understood. we performed a prospective age-stratified study in outpatient clinics investigating the prevalence of df; 578 patients were enrolled, and 317 patients with a negative rapid diagnostic test (rdt) for malaria were tested for dengue. malaria was confirmed in 52% (301/578, 95% confidence interval [ci] = 48-56%), ...021734138
mosquitocidal properties of igg targeting the glutamate-gated chloride channel in three mosquito disease vectors (diptera: culicidae).the glutamate-gated chloride channel (glucl) is a highly sensitive insecticide target of the avermectin class of insecticides. as an alternative to using chemical insecticides to kill mosquitoes, we tested the effects of purified immunoglobulin g (igg) targeting the extracellular domain of glucl from anopheles gambiae (agglucl) on the survivorship of three key mosquito disease vectors: anopheles gambiae s.s., aedes aegypti and culex tarsalis. when administered through a single blood meal, anti-a ...025994632
evaluation of carbon dioxide- and 1-octen-3-ol-baited centers for disease control fay-prince traps to collect aedes albopictus.during the summer of 2001, field studies were performed to evaluate the effects of carbon dioxide (co2) and 1-octen-3-ol (octenol) on the ability to collect aedes albopictus with centers for disease control (cdc) fay-prince traps. results from these studies indicated that ae. albopictus is significantly more attracted to co2- or co2 + octenol-baited cdc fay-prince traps than unbaited or octenol-baited traps. however, the difference between the responses to co2 and co2 + octenol was not statistic ...014710753
epidemic infections and their relevance to the gulf and other arabian peninsula countries. 024019727
preliminary analysis of several attractants and spatial repellents for the mosquito, aedes albopictus using an olfactometer.mosquito attractants and spatial repellents hold great promise in controlling mosquito pests. in assessing the effectiveness of mosquito attractants and repellents, a good olfactometer system, and optimized testing conditions, are essential. in this research, we demonstrated the usefulness of an olfactometer system, and optimized testing conditions for aedes albopictus (diptera: culicidae). we found no significant difference in the biting activity of the insect between 8:00 and 22:00. furthermor ...023418948
diversity of wolbachia pipientis strain wpip in a genetically admixtured, above-ground culex pipiens (diptera: culicidae) population: association with form molestus ancestry and host selection patterns.analysis of molecular genetic diversity in nine marker regions of five genes within the bacteriophage wo genomic region revealed high diversity of the wolbachia pipentis strain wpip in a population of culex pipiens l. sampled in metropolitan chicago, il. from 166 blood fed females, 50 distinct genetic profiles of wpip were identified. rarefaction analysis suggested a maximum of 110 profiles out of a possible 512 predicted by combinations of the nine markers. a rank-abundance curve showed that fe ...022679853
effects of temperature on emergence and seasonality of west nile virus in california.temperature has played a critical role in the spatiotemporal dynamics of west nile virus transmission throughout california from its introduction in 2003 through establishment by 2009. we compared two novel mechanistic measures of transmission risk, the temperature-dependent ratio of virus extrinsic incubation period to the mosquito gonotrophic period (bt), and the fundamental reproductive ratio (r(0)) based on a mathematical model, to analyze spatiotemporal patterns of receptivity to viral ampl ...022556092
analysis of post-blood meal flight distances in mosquitoes utilizing zoo animal blood meals.we assessed the post-blood meal flight distance of four mosquito species in a unique environment using blood meal analysis. mosquitoes were trapped at the rio grande zoo in albuquerque, nm, and the blood source of blood-engorged mosquitoes was identified. the distance from the enclosure of the animal serving as a blood source to the trap site was then determined. we found that mosquitoes captured at the zoo flew no more than 170 m with an average distance of 106.7 m after taking a blood meal. th ...022548540
in search of a better mosquito repellent. 018709154
survival of west nile virus-challenged southern house mosquitoes, culex pipiens quinquefasciatus, in relation to environmental temperatures.we investigated the effect of west nile virus (wnv) infection on survival in two colonies of culex pipiens quinquefasciatus say (diptera: culicidae) originating from vero beach and gainesville, fl. mosquitoes were fed west nile virus-infected blood and checked daily for survival. exposure to wnv decreased survival among cx. p. quinquefasciatus from gainesville relative to unexposed individuals at 31° c. in contrast, exposure to wnv enhanced survival among cx. p. quinquefasciatus from vero beach ...024820564
zika virus, french polynesia, south pacific, 2013. 024856001
aedes (stegomyia) aegypti and aedes (howardina) cozumelensis in yucatán state, méxico, with a summary of published collection records for ae. cozumelensis.we collected mosquito immatures from artificial containers during 2010-2011 from 26 communities, ranging in size from small rural communities to large urban centers, located in different parts of yucatán state in southeastern méxico. the arbovirus vector aedes (stegomyia) aegypti was collected from all 26 examined communities, and nine of the communities also yielded another container-inhabiting aedes mosquito: aedes (howardina) cozumelensis. the communities from which ae. cozumelensis were coll ...023181861
public health response to aedes aegypti and ae. albopictus mosquitoes invading california, usa.aedes aegypti and ae. albopictus mosquitoes, primary vectors of dengue and chikungunya viruses, were recently detected in california, usa. the threat of potential local transmission of these viruses increases as more infected travelers arrive from affected areas. public health response has included enhanced human and mosquito surveillance, education, and intensive mosquito control.026401891
regulation of physiological processes by micrornas in insects.micrornas (mirnas) are small non-coding rnas that function in gene regulatory processes in plants and animals by targeting sites within messenger rna. in insects, mirnas have been shown to regulate a variety of physiological processes throughout insect development, including molting, metamorphosis, oogenesis, embryogenesis, behavior and host-pathogen interactions. the roles of mirnas in the model organism, drosophila melanogaster, have been studied extensively due to the conserved nature of mirn ...026251827
next-generation sequencing in clinical virology: discovery of new viruses.viruses are a cause of significant health problem worldwide, especially in the developing nations. due to different anthropological activities, human populations are exposed to different viral pathogens, many of which emerge as outbreaks. in such situations, discovery of novel viruses is utmost important for deciding prevention and treatment strategies. since last century, a number of different virus discovery methods, based on cell culture inoculation, sequence-independent pcr have been used fo ...026279987
hla class ii allele polymorphism in an outbreak of chikungunya fever in middle andaman, india.a sudden upsurge of fever cases with joint pain was observed in the outpatient department, community health centre, rangat during july-august 2010 in rangat middle andaman, india. the aetiological agent responsible for the outbreak was identified as chikungunya virus (chikv), by using rt-pcr and igm elisa. the study investigated the association of polymorphisms in the human leucocyte antigen class ii genes with susceptibility or protection against chikv. one hundred and one patients with clinica ...023710940
modeling and biological control of mosquitoes.models can be useful at many different levels when considering complex issues such as biological control of mosquitoes. at an early stage, exploratory models are valuable in exploring the characteristics of an ideal biological control agent and for guidance in data collection. when more data are available, models can be used to explore alternative control strategies and the likelihood of success. there are few modeling studies that explicitly consider biological control in mosquitoes; however, t ...017853610
habitat segregation of mosquito arbovirus vectors in south florida.oviposition traps set in rural to urban environments in three south florida counties were colonized predominantly by culex quinquefasciatus say (35.1%), aedes albopictus (skuse) (34.5%), aedes aegypti (l.) (23.8%), and culex nigripalpus theobald (6.6%) during 1 yr of monthly sampling. significant differences were detected among counties for abundances of cx. quinquefasciatus and for percentage composition of that species and ae. albopictus. aerial images of habitats around each collection site w ...017162945
urbanization and geographic expansion of zoonotic arboviral diseases: mechanisms and potential strategies for prevention.arthropod-borne viruses (arboviruses) mainly infect people via direct spillover from enzootic cycles. however, dengue, chikungunya, and yellow fever viruses have repeatedly initiated urban transmission cycles involving human amplification and peridomestic mosquito vectors to cause major epidemics. here, i review these urban emergences and potential strategies for their prevention and control.023910545
antiviral natural products and herbal medicines.viral infections play an important role in human diseases, and recent outbreaks in the advent of globalization and ease of travel have underscored their prevention as a critical issue in safeguarding public health. despite the progress made in immunization and drug development, many viruses lack preventive vaccines and efficient antiviral therapies, which are often beset by the generation of viral escape mutants. thus, identifying novel antiviral drugs is of critical importance and natural produ ...024872930
interspecific competition of a new invasive mosquito, culex coronator, and two container mosquitoes, aedes albopictus and cx. quinquefasciatus (diptera: culicidae), across different detritus environments.the mosquito culex coronator (dyar and knab) (diptera: culicidae) has undergone rapid range expansion in the united states since 2003, with its historical distribution in the southwest expanding eastward to the atlantic coast. although cx. coronator nominally use small natural aquatic habitats for development, the use of containers (e.g., tires) makes it potentially important as container invasive. to determine the potential ecological effects of cx. coronator on resident container species, we c ...024605457
high degree of single nucleotide polymorphisms in california culex pipiens (diptera: culicidae) sensu lato.resolution of systematic relationships among members of the culex pipiens (l.) complex has important implications for public health as well as for studies on the evolution of sibling species. currently held views contend that in california considerable genetic introgression occurs between cx. pipiens and cx. quinquefasciatus say, and as such, these taxa behave as if they are a single species. development of high throughput snp genotyping tools for the analysis of cx. pipiens complex population s ...022493847
no evolutionary response to four generations of laboratory selection on antipredator behavior of aedes albopictus: potential implications for biotic resistance to invasion.aedes albopictus (skuse) is an invasive container-dwelling mosquito and an important disease vector that co-occurs with the native mosquito, aedes triseriatus (say), and the predatory midge, corethrella appendiculata (grabham). larval ae. triseriatus show significantly greater antipredatory responses when compared to larval ae. albopictus in the presence of predation cues from c. appendiculata. the potential for evolution of antipredatory behavioral responses to c. appendiculata in ae. albopictu ...019645279
emerging and re-emerging viruses in the era of globalisation. 019657478
effects of ingested vertebrate-derived factors on insect immune responses.during the process of blood feeding insect vectors are exposed to an array of vertebrate-derived blood factors ranging from byproducts of blood meal digestion to naturally occurring products in the blood including growth hormones, cytokines and factors derived from blood-borne pathogens themselves. in this review, we examine the ability of these ingested vertebrate blood factors to alter the innate pathogen defenses of insect vectors. the ability of these factors to modify the immune responses o ...025401083
towards a hybrid agent-based model for mosquito borne disease.agent-based models (abm) are used to simulate the spread of infectious disease through a population. detailed human movement, demography, realistic business location networks, and in-host disease progression are available in existing abms, such as the epidemic simulation system (episims). these capabilities make possible the exploration of pharmaceutical and non-pharmaceutical mitigation strategies used to inform the public health community. there is a similar need for the spread of mosquito bor ...026618203
outsmarting olfaction: the next generation of mosquito repellents. 016002364
passive prophylaxis with permethrin-treated tents reduces mosquito bites among north american summer campers.over 2 million adolescents participate in summer-camp experiences, placing themselves at risk for mosquito-borne illness. insect repellent is recommended but often not used. it is unknown whether permethrin treatment of a location, such as a campsite, provides passive prophylaxis reducing mosquito bites among all persons in the immediate vicinity.015813141
functional circadian clock genes are essential for the overwintering diapause of the northern house mosquito, culex pipiens.the short day lengths of late summer are used to program the overwintering adult diapause (dormancy) of the northern house mosquito, culex pipiens. here, we investigated the role of clock genes in initiating this diapause and asked whether the circadian cycling of clock gene expression persists during diapause. we provide evidence that the major circadian clock genes continue to cycle throughout diapause and after diapause has been terminated. rna interference (rnai) was used to knock down the c ...025653422
facts and ideas from anywhere. 016369660
evaluation of a stable isotope method to mark naturally-breeding larval mosquitoes for adult dispersal studies.understanding mosquito dispersal is critically important for vector-borne disease control and prevention. mark-release-recapture methods using various marking techniques have made substantial contributions to the study of mosquito biology. however, the ability to mark naturally breeding mosquitoes noninvasively and with life-long retention has remained problematic. here, we describe a method to mark naturally breeding mosquitoes with stable isotopes. culex pipiens f. molestus mosquitoes were pro ...022308772
dengue outbreak in key west, florida, usa, 2009.after 3 dengue cases were acquired in key west, florida, we conducted a serosurvey to determine the scope of the outbreak. thirteen residents showed recent infection (infection rate 5%; 90% ci 2%-8%), demonstrating the reemergence of dengue in florida. increased awareness of dengue among health care providers is needed.022257471
volatile phytochemicals as mosquito semiochemicals.plant biochemical processes result in the release of an array of volatile chemical substances into the environment, some of which are known to play important plant fitness enhancing functions, such as attracting pollinators, thermal tolerance of photosynthesis, and defense against herbivores. cunningly, phytophagous insects have evolved mechanisms to utilize these volatiles to their own advantage, either to colonize a suitable host for feeding, reproduction and oviposition or avoid an unsuitable ...025383131
risk assessment for yellow fever in western and north-western provinces of zambia.north-western and western provinces of zambia were reclassified as low-risk areas for yellow fever (yf). however, the current potential for yf transmission in these areas is unclear.025722614
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