Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| specific igg and ige responses to dermatophagoides pteronyssinus in sprague-dawley rats. | specific igg and ige antibodies against the mite dermatophagoides pteronyssinus (dp) have been determined in the serum of sprague dawley (sd) rats by enzyme linked immunosorbent assay (elisa) and passive cutaneous anaphylaxis (pca), respectively. immunization of rats with dp produces a rapid increase of serum specific ige antibody levels which decrease when specific igg antibodies start rising. the results indicate that the sd rat is a good model to induce a humoral immune response to allergen d ... | 1990 | 2099531 |
| expression of dermatophagoides pteronyssinus allergen, der p ii, in escherichia coli and the binding studies with human ige. | lambda gt11 clones expressing the major house dust mite allergen, der p ii, have been reported to react with ige in the serum of a high proportion of allergic patients. the clones described, however, only produced small quantities of protein which was not fused to the beta-galactosidase of the vector. a construct of the der p ii is described which produces a fusion of der p ii, minus its leader sequence, with the glutathione-s transferase in the pgex vector. this could be readily isolated and wa ... | 1990 | 2187817 |
| comparison between rast and pharmacia cap system: a new automated specific ige assay. | rast represents the standard technique to titrate serum-specific ige and was found to have a higher efficiency in the diagnosis of ige-mediated allergy than other in vitro tests. pharmacia cap system (cap) is a new solid-phase immunoassay, fully automated, used for the titration of specific ige antibodies. results are listed in kilounits per liter, equilibrated against the world health organization standard for ige. rast and cap were compared in 106 unselected patients (6 to 59 years) characteri ... | 1990 | 2191989 |
| comparison of a multi-allergen dipstick ige assay to skin-prick test and rast. | a multi-allergen dipstick enzyme immunoassay 'quidel allergy screen' (qas) has recently been developed commercially for measuring ige antibodies against nine allergens (house dust 1, house dust 2, dermatophagoides pteronyssinus, d. farinae, japanese cedar, ragweed, cat dander, sweet vernal grass, and egg white) at one time. to assess whether this assay is useful in screening allergen-specific ige antibody, we compared the titres of ige antibodies against the nine allergens measured by qas to tho ... | 1990 | 2192782 |
| the control of house dust mite allergens in rugs. | used rugs were vacuum cleaned, "wet cleaned," "shampooed," or heated in an autoclave. the effects on the live-mite population, on the allergen (dermatophagoides pteronyssinus, der p i and der p ii; and dermatophagoides farinae, der f ii) content, and on the suitability of the rugs as a habitat for house dust mites was studied. autoclaving was most effective in all three aspects. the cleaning methods were quite inefficient for killing the mites, but a significant reduction of allergens and deteri ... | 1990 | 2229844 |
| antigenic analysis of group i house dust mite allergens using random fragments of der p i expressed by recombinant dna libraries. | antigenic regions of a major house dust mite allergen, der p i, were identified by a recombinant dna strategy employing the technique of random fragmentation. fragments of cdna coding for der p i were produced by sonication and used to construct lambda gt 11 expression libraries. analyses of recombinant fragments reactive with a rabbit anti-der p i antiserum showed that the b cell determinants expressed in escherichia coli were limited, with the majority (86%) of antigenic clones isolated mappin ... | 1990 | 2246073 |
| [results of patch test using mite components in atopic dermatitis (ad) patients. 1st report. "as is patch test" using crushed mites]. | as is patch test using crushed live mites and crushed dried dead mites of dermatophagoides pteronyssinus (d.p.), dermatophagoides farinae (d.f.), & tryophagus ptrescentiae (t.p.) was performed on 49 ad patients and 18 control patients suffering from dermatitis other than ad. results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 ad patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases ... | 1990 | 2273575 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| the prevalence of allergic disease and ige antibodies to house dust mite in schoolchildren in taiwan. | the prevalence of positive specific ige antibodies to house dust mites (dermatophagoides pteronyssinus; d. farinae) was determined by enzyme-linked immunosorbent assay (elisa) in 5097 (61%) volunteers of 8345 schoolchildren aged between 7 and 14 yr from two government schools. all of them filled out a questionnaire concerning allergic symptoms. among them, 412 (8.1%) children showed a positive reaction to at least one of the two mite allergens, the range varying between 5.6 and 11.2% according t ... | 1990 | 2310980 |
| [seasonal variations in house dust mite allergens--significance for children with allergy]. | allergenic proteins of the house dust mites dermatophagoides pteronyssinus and dermatophagoides farinae and their major allergens der p i and der f i are one of the most significant environmental factors which may facilitate sensitization and allergic airway disease in childhood. in order to measure seasonal variations of mite allergen exposure, we collected mattress dust samples monthly in 13 homes over a period of 1 year. der p i and der f i were determined with a sandwich type elisa. mite all ... | 1990 | 2320013 |
| dermatophagoides and storage mites: statistical analysis of rast results. | r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity. | 1990 | 2321810 |
| localization of antigens and allergens in thin sections of the house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae). | cryostat sections of the house dust mite, dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, der p i. sections also were probed for allergens with sera from human mite-allergic subjects containing ige antibodies to mite allergens and with lectins. antibody binding was mainly to the gut lining and gut contents of the mite, although some specific labeling also ... | 1990 | 2332879 |
| isolation of cdna coding for the major mite allergen der p ii by ige plaque immunoassay. | a lambda gt11 library made with cdna from the house dust mite dermatophagoides pteronyssinus was screened with human allergic serum by ige plaque radioimmunoassay. this resulted in the isolation of clones coding for the major allergen der p ii. the cdna coded for a 129-residue protein of 14,131 daltons with no n-glycosylation sites. no sequence homology with other proteins was evident. the der p ii expressed in escherichia coli reacted with ige in 14 of 17 sera from mite-allergic patients giving ... | 1990 | 2341191 |
| immune response to blomia kulagini and dermatophagoides pteronyssinus in sweden and brazil. | the immune responses to the non-pyroglyphid mite blomia kulagini and the pyroglyphid mite dermatophagoides pteronyssinus were compared in 440 swedish farmers and 34 allergic subjects from brazil using the radioallergosorbent test (rast). both b. kulagini and d. pteronyssinus were shown to be a common cause of sensitization, particularly in brazil. the highest rast scores were found against d. pteronyssinus in sera from both countries. allergenic cross-reactivity between the two mites was studied ... | 1990 | 2341199 |
| a study of the relationship between childhood nephrotic syndrome and allergic diseases. | the incidence of atopic diseases in 206 children with nephrotic syndrome (ns) was studied. boys with ns had three times higher incidence of bronchial asthma than the general population. there was no difference in the girls. both boys and girls with ns had about three times more allergic rhinitis and ten times more atopic dermatitis than the general population. in ns patients with associated allergic disease, skin test and allergen-specific ige antibodies by radioallergosorbent test (rast) were p ... | 1990 | 2347227 |
| changes of serum-specific ige antibody titer during hyposensitization in mite-sensitive asthmatic children. | in this study, we used the enzyme-linked immunosorbent assay (elisa) to evaluate the changes of ige antibody titer against dermatophagoides pteronyssinus (d. pteronyssinus) or dermatophagoides farinae (d. farinae) in asthmatic children after immunotherapy. according to elisa analysis, a significantly higher mean level of ige antibody titer to d. pteronyssinus (or d. farinae) was found in nonhyposensitized asthmatic children than in the pediatric control group (p less than 0.001), but there was n ... | 1990 | 2347816 |
| assessment and control of house dust mite infestation. | most allergens of the house dust mite (dermatophagoides pteronyssinus and dermatophagoides farinae) are carried by the mite excreta rather than by the mites themselves. a method of assessing the concentration of mite excreta in dust (the acarex test) is described. the effects of acarosan products, whose active ingredient is solidified benzyl benzoate, on mites and mite excreta were evaluated. over a period of three years, three to four applications of acarosan effectively destroyed mites in carp ... | 1990 | 2379225 |
| functional characterization of lymphocyte response to fractionated house dust mite antigens (dermatophagoides pteronyssinus) in atopic and non-atopic individuals. | this study investigated the cellular response of human lymphocytes to dermatophagoides pteronyssinus crude antigen and 14 molecular weight (mw) fractions. the cells were derived from atopic patients and healthy individuals who were skin test-positive or skin test-negative to mite antigen. when stimulated with crude antigen, the group of patients showed elevated proliferation and production of lymphokine in comparison with the healthy skin test-negative individuals (p less than 0.01). by stimulat ... | 1990 | 2379943 |
| prevalence of specific ige to storage mites (a. siro, l. destructor and t. longior) in an urban population and crossreactivity with the house dust mite (d. pteronyssinus). | the role of pyroglyphid mites in house dust allergy is well established and the major allergens from the common house dust mites (dermatophagoides species) have been characterized. there is, however, relatively little progress in the understanding of the human ige response to non-pyroglyphid storage mites, allergenic crossreactivity with other mite species and extent of environmental exposure. we studied 196 individuals from an urban environment who were not occupationally exposed to storage mit ... | 1990 | 1695869 |
| human basophil releasability. vi. changes in basophil releasability in patients with allergic rhinitis or bronchial asthma. | we evaluated basophil releasability in two groups of allergic patients with positive skin tests to dermatophagoides pteronyssinus major allergen (der p l) (29 adults with bronchial asthma and 17 with allergic rhinitis) and in 31 age-matched normal donors. both basophil reactivity (maximal percent histamine release) and basophil sensitivity (the concentration that causes 50% of maximal percent histamine release: hc50) to der p l in patients with asthma were similar to those in patients with aller ... | 1990 | 1700653 |
| cross-antigenicity between the scabies mite, sarcoptes scabiei, and the house dust mite, dermatophagoides pteronyssinus. | this study demonstrated that antigens of the parasitic mite sarcoptes scabiei (ss) cross-react with antigens of the house dust mite dermatophagoides pteronyssinus (dp). crossed immunoelectrophoresis (cie) reaction of ss extract with rabbit anti-dp serum resulted in multiple immunoprecipitates. reciprocal cie reactions gave similar results. immunoprecipitates from both reactions bound ige in the sera of dust-mite-sensitive patients who had no history of scabies. sodium dodecyl sulfate-polyacrylam ... | 1991 | 1705952 |
| the characteristics of antigen define the cellular source and kinetics of histamine-releasing factor induced by human peripheral-blood mononuclear cells. | peripheral-blood leucocytes from dermatophagoides pteronyssinus- and/or lolium perenne-allergic patients produce in vitro histamine-releasing factor (hrf) in an allergen-specific and concentration-dependent relationship. maximum production of hrf occurred in cultures containing as little as 10-100 pg/ml crude allergen extract, although a second peak occurred at higher concentrations (10-100 micrograms/ml). while hrf was detectable in 1-hour cultures, maximal production required 24 h in culture. ... | 1991 | 1718885 |
| epitope mapping of the dermatophagoides pteronyssinus house dust mite major allergen der p ii using overlapping synthetic peptides. | fourteen synthetic peptides of 15 amino acid residues length, overlapping by five residues and spanning the entire sequence of the major allergen der p ii from the house dust mite dermatophagoides pteronyssinus were synthesized. these peptides were coupled to cnbr-activated sepharose-4b and used as solid-phase antigens in epitope mapping studies using human ige antisera. these antibodies bound predominantly to the peptide comprising residues 65-78, the binding of which was inhibited by native de ... | 1991 | 1720504 |
| mucosal immunity in primary glomerulonephritis: ii. study of the serum iga subclass repertoire to food and airborne antigens. | iga specific for 7 food and 6 airborne antigens were sought in the serum of 30 adult patients with iga mesangial nephropathy (iga gn), 23 with membranous nephropathy (mgn), 20 with idiopathic nephrotic syndrome (ins), 11 with membranoproliferative gn (mpgn) and 22 healthy controls by means of an enzyme-linked immunoassay. the iga subclass was determined using monoclonal antibodies. increased levels of iga specific for gliadin, bovine serum albumin (bsa), ovalbumin, lysozyme and alpha-lactalbumin ... | 1991 | 1766494 |
| asthma in tanta, egypt: serologic analysis of total and specific ige antibody levels and their relationship to parasite infection. | the relationship between asthma, ige and parasite infection was compared in 68 randomly selected patients with asthma and 37 nonasthmatic controls living in tanta, egypt. sera were assayed for total ige and for ige antibodies to inhaled allergens (mite, cat, cockroach, ryegrass, ragweed and 3 fungi) and to parasite antigens (schistosoma mansoni and brugia malayi). parasite infection was determined by microscopic examination of stool specimens. total ige levels were significantly higher in patien ... | 1991 | 1809691 |
| allergen- and bacterial antigen-specific t-cell clones established from atopic donors show a different profile of cytokine production. | we have established a large panel of t-cell clones (tccs) specific for dermatophagoides pteronyssinus and lolium perenne group i grass pollen allergens (total, 61) and for tetanus toxoid and protein purified derivative bacterial antigens (total, 38) from the peripheral blood of two atopic individuals and then analyzed their ability to produce interleukin 4 (il-4), il-5, and interferon gamma (ifn-gamma). upon stimulation with phorbol 12-myristate 13-acetate plus anti-cd3 antibody, the great major ... | 1991 | 1827920 |
| analysis of house dust mite-specific ige, igg4, and igg antibodies during immunotherapy in asthmatic children. | house dust mite (dermatophagoides pteronyssinus, dp) specific ige, igg4, and igg antibodies were analyzed by western blotting in 18 asthmatic children during the course of 2-years' allergen-specific immunotherapy. although more than 50 protein bands could be identified in the crude mite extracts by sds-page, only eight of them elicited both ige and igg4 antibodies in most patients. the molecular weights were 110, 60, 55, 43, 33, 27, 16, and 14 kd, respectively. no antibodies against new antigens ... | 1991 | 1859043 |
| identification of major allergens from the house dust mites, dermatophagoides farinae and dermatophagoides pteronyssinus, by electroblotting. | the allergens were separated from the extracts of house dust mites by sds-polyacrylamide gel electrophoresis (sds-page) and identified by autoradiography. over 30 protein bands of the whole body extract of dermatophagoides farinae were apparent on 10-20% gradient sds-page, and 13 bands with mw between 93kd and 12kd bound with specific ige antibodies in patients' sera sensitive to house dust mites. the major allergenic component of the whole body extract of d. farinae was the protein of mw 14-15k ... | 1991 | 1877252 |
| diagnosis of nasal allergy to the house dust mite. | twenty-five patients with perennial rhinitis and a positive skin prick test (spt) for dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) were submitted to nasal provocation and the radioimmunosorbent test (rast) for specific ige-antibodies. we found a significant correlation in the reaction to both allergen extracts for all parameters examined. in addition, there was a significant correlation among the spt, the rast and the nasal provocation for dp and between the spt and the ... | 1991 | 1891679 |
| exposure and sensitization to dust mite allergens among asthmatic children in são paulo, brazil. | a group of 20 mite allergic asthmatic children aged 6-12 years old, living in são paulo, brazil, was studied regarding their degree of sensitization to house dust mites and exposure to mite allergens in their homes. in 18 out of 20 houses at least one dust sample was obtained which contained greater than 10 micrograms der p i/g of dust. the highest levels of dermatophagoides pteronyssinus allergens, der p i and group ii, were measured in bedding samples (geometric mean 38.4 and 36.6 micrograms/g ... | 1991 | 1913266 |
| [immunologic response to microorganisms from a polluted ventilation system in a working environment]. | authors point out the morbidity of employees working in ventilation systems contaminated with various microorganisms. they analysed 96 workers exposed to air conditioning system (group a), and 71 workers (group b) breathing normal ambient air. the workers of both groups were subjected clinically by functionally and immunologically. preparation of antigens "mmm" (monday morning miseries) was used as an original method by ajello et al. for producing antigens from systemic mycotic agents and subseq ... | 1991 | 1916435 |
| [the significance of individual inhalation allergens in the sensitization of children with respiratory allergy]. | four hundred children were skin tested with following allergens: dermatophagoides pteronyssinus, moulds, animal dander (mixture of dog, cat, sheep and horse dander), tree pollens, weed pollen (ambrosia artemisifolia), and six grass pollens, as well as three nutritive allergens in children less than three years of age. results demonstrated that in 200 children with perenial rhinitis and/or asthma. dermatophagoides gave positive results in over 90%. in 200 children with pollen allergy, grasses wer ... | 1991 | 1916444 |
| nutritional value of yeast for dermatophagoides pteronyssinus (acari: epidermoptidae) and the antigenic and allergenic composition of extracts during extended culturing. | cultures of european house dust mite, dermatophagoides pteronyssinus (trouessart), were evaluated with respect to yeast nutritional value, together with an assessment of the antigenic and allergenic composition. cie analysis of extracts illustrated that yeast contributes many antigens throughout the rearing period. however, there is an indication of the consumption of only one particular yeast antigen by the mites. total protein content profile of the mite cultures is shown in addition to the to ... | 1991 | 1941906 |
| diagnostic performance characteristics of the standard phadebas rast, modified rast, and pharmacia cap system versus skin testing. | results from three in vitro assays for allergen-specific ige, the standard phadebas radioallergosorbent test (phrast), modified rast (mrast), and the new pharmacia cap system (cap) were compared with skin prick testing (spt) results in 104 patients with allergic rhinitis and/or asthma and 24 nonatopic controls. five allergens were evaluated: cat, dermatophagoides pteronyssinus, alternaria, june grass, and short ragweed. using spt results as the reference standard, the phrast had the lowest sensi ... | 1991 | 1958005 |
| preparation of specific monoclonal antibodies against der f ii one of the major allergens of dermatophagoides farinae. | five monoclonal antibodies (mabs) against der f ii, one of the major allergens of dermatophagoides farinae, were obtained from mice immunized with the allergen. western blotting analysis revealed that only one mab, named 15e11, was specific for der f ii, whereas the other mabs crossreacted with der p ii, one of the major allergens of dermatophagoides pteronyssinus. these results indicate the possibility that the der f ii-specific mab, 15e11, is a useful reagent for specific determination of der ... | 1991 | 1958138 |
| caffeine, a naturally occurring acaricide. | since caffeine is a plant alkaloid that has been described as a naturally occurring insecticide, its acaricidal effect on dermatophagoides pteronyssinus (dp) was investigated. twelve cultures were established by adding 30 dp to 200 mg of tetramin fish food and brewer's yeast (8:2 ratio); six cultures were treated with 20 mg of finely ground caffeine. all 12 cultures were incubated at 75% relative humidity, 25 degrees c, and observed during 8 weeks. live mites were then counted under a stereoscop ... | 1991 | 1991914 |
| house dust mites in the west indies. | the purpose of this study was to determine the role of the house dust mite. dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df), in allergic diseases on the island of st. lucia. dust samples were taken from living quarters of patients and hospital volunteers. the level of df antigen in these living quarters was measured by rast inhibition. all samples showed df antigen within a large range of values. prick tests were done to house dust mite and molds on 91 patients with suspect ... | 1991 | 2014932 |
| detection of b-lymphocytes secreting antibodies to dermatophagoides antigens. | an enzyme-linked immunospot assay (eli-spot assay) has been established to count individual cells secreting antibodies to dermatophagoides spp. allergens. initial optimization of the assay was performed using der p i-specific murine hybridoma cell lines. inhibition with soluble purified allergen demonstrated that spot formation was specific. addition of cycloheximide showed that spot formation was due to antibodies actively produced and secreted and not caused by preformed surface-associated ig. ... | 1991 | 2021882 |
| house dust acarofauna and der p i equivalent in australia: the relative importance of dermatophagoides pteronyssinus and euroglyphus maynei. | the acarofauna and der p i allergen concentrations in dust samples from mattresses and lounge room carpets obtained from 20 homes from two coastal cities. perth and bunbury, were determined. all samples were shown to contain mites and the geometric mean numbers of total mites/g of mattress and carpet dust for perth and bunbury were 480 and 263, and 585 and 992, respectively. carpets from both centres had a significantly (p less than 0.02) greater mean number of mite species (perth 9.1, bunbury 9 ... | 1991 | 2043990 |
| ipd-1151t: a prototype drug for ige antibody synthesis modulation. | ipd-1151t [(+/-)-[2-[4-(3-ethoxy-2-hydroxypropoxy)phenylcarbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate] inhibits not only antigen-induced histamine release from mast cells but also ige antibody formation. the present paper describes the inhibitory effect of ipd-1151t on the ige antibody formation. the ige antibody formation in balb/c mice which had been immunized with dinitrophenylated ascaris extract (dnp.as) plus alum was inhibited dose-dependently by ipd-1151t given p.o. the formation ... | 1991 | 1665310 |
| skin cross-reactivity between dermatophagoides pteronyssinus and storage mites in atopic children. | in our environment, some children with allergic respiratory pathology do not have as favorable an evolution as expected after three years of immunotherapy with d. pteronyssinus extracts. we believe this is due to the existence of other mites whose antigenic determinants are not present in d. pteronyssinus. the purpose of the present study was to evaluate skin cross-reactivity between dermatophagoides and storage mites in children with bronchial asthma. fifty patients were chosen, with a mean age ... | 1991 | 1669575 |
| estimation of ige antibodies to the common allergens by reverse enzyme immunoassay. comparison with the radioallergosorbent test. | a reverse enzyme immunoassay (reina) is described, in which polystyrene microtiter wells are sensitized with murine monoclonal anti-human ige, and then sequentially allowed to react with patient's serum, peroxidase-labeled allergens and substrate. the results obtained with the sera of patients allergic to lolium perenne grass pollen, the tree pollens of betula alba and olea europea, the epithelia of cat and dog, the mite dermatophagoides pteronyssinus, or to the foodstuffs cow's milk, chicken eg ... | 1991 | 1669584 |
| human atopen-specific types 1 and 2 t helper cell clones. | eight representative t lymphocyte clones (tlc) randomly selected from previously described panels of cd4+ housedust mite dermatophagoides pteronyssinus (dp)-specific tlc from atopic and nonatopic donors were studied in more detail in a comparative investigation. the tlc from the atopic donors closely resembled murine type 2 th (th2) cells by secreting substantial il-4, il-5, il-6, tnf-alpha, and granulocyte-macrophage (gm)-csf, minimal ifn-gamma, and relatively little il-2. in contrast, the nona ... | 1991 | 1680923 |
| increased numbers of th2-like cd4+ t cells in target organs and in the allergen-specific repertoire of allergic patients. possible role of il-4 produced by non-t cells. | phytohemagglutinin (pha)-induced human t cell clones (tcc) derived from conjunctival flogistic tissues of 3 patients with vernal conjunctivitis produced unusually high amounts of interleukin-4 (il-4) and no, or limited amounts of, gamma-interferon (ifn-gamma). allergen (dermatophagoides pteronyssinus or lolium perenne group i)-specific tcc derived from peripheral blood of two atopic donors produced significantly higher amounts of il-4 and significantly lower amounts of ifn-gamma than tcc specifi ... | 1991 | 1682270 |
| aberrant t cell regulation of ige production in atopy. | atopy is associated with elevated serum levels of allergen-specific immunoglobulin e (ige). ige production is preferentially induced by the t cell-derived lymphokine interleukin-4 (il-4) and is suppressed by interferon and prostaglandins, of which interferon-gamma (ifn-gamma) is produced by t cells. we review our studies on the question of whether atopy is associated with cd4+ allergen-specific t cells with an unbalanced lymphokine production. we prepared panels of housedust mite (dermatophagoid ... | 1991 | 1683236 |
| [sensitization against storage mites in allergic rhinopathy]. | in the past 15 years, storage mites became more common as an agent in allergic airway disease. sensitization to storage mites has been reported in farmers and grain workers. there were some studies investigating local populations, especially in the north of europe. the sensitization to storage mites in a rural population seems to be similar to the rate found for dermatophagoides. the main diseases caused by storage mites are allergic rhinitis and asthma. although there is a cross-reactivity betw ... | 1991 | 1755912 |
| [sensitization to granary mites in patients with allergic diseases]. | as many as 71 patients selected from a group of 550 patients suffering from allergic diseases, sensitized to the house dust mites dermatophagoides pteronyssinus were examined. a study was made of the presence of allergen-specific ige antibodies against house dust mites, storage mites (7 species altogether) using allergenic discs and commercial kits rast (pharmacia, sweden). the group under examination mostly manifested sensitization to the house dust mites: dermatophagoides ferinae (80%), eurogl ... | 1991 | 2063323 |
| [relationship between immediate hypersensitive reactions by buckwheat ingestion and specific ige for rice in subject with positive ige-rast for buckwheat]. | ige-mediated mechanisms are important in immediate hypersensitive reactions (ihr) to buckwheat. however, a part of subjects with high ige for buckwheat show no ihr to buckwheat ingestion. inspite of cross-allergenicity between buckwheat and rice, rice ingestion rarely induces ihr even in subjects with high ige for rice unlike buckwheat-induced ihr. we speculated that there were some relationships between the presence of ihr to buckwheat and recognition of cross-allergenic determinants on buckwhe ... | 1991 | 1793368 |
| local immunotherapy by inhalation of a powder extract in asthma due to house dust mite dermatophagoides pteronyssinus: a double-blind comparison with parenteral immunotherapy. | to verify the efficacy and the tolerability of local immunotherapy (li) by inhalation of a powder extract of house dust mite (hdm), in comparison with parenteral immunotherapy (pi) by injection, 10 patients with asthma due to hdm were studied in a blind fashion. 5 patients (group a) underwent li and subcutaneous injections of placebo, 5 patients (group b) underwent pi and inhalation of lactose for 6 months. in both groups each inhalation was preceded by premedication with disodium cromoglycate ( ... | 1991 | 1669598 |
| in vivo release of 15-hete and other arachidonic acid metabolites in nasal secretions during early allergic reactions. | the purpose of this study is to examine the "in vivo" release of 15-hete and other arachidonic acid metabolites in nasal secretions following a challenge with "dermatophagoides pteronyssinus" in patients with allergic rhinitis and non-allergic controls. in addition, we examine the effects of a membrane stabilizer, such as sodium cromoglycate, on these metabolites. thirteen allergic subjects and seven healthy controls are studied. 15-hete, peptide leukotrienes, ltb4, pgd2, pge2 and pgf2 alpha lev ... | 1991 | 1662405 |
| increased secretion of tumor necrosis factor alpha and interleukin-6 by alveolar macrophages consecutive to the development of the late asthmatic reaction. | the late asthmatic reaction (lar), consecutive to bronchial allergen challenge, is characterized both by the influx of various cells in proximal and distal airways and by the enhancement of bronchial hyperresponsiveness. however, the exact conditions for the development of the inflammatory reaction during the lar remain to be specified. since monokines play a key role in inflammatory processes, particularly in the lung, the production of tumor necrosis factor-alpha (tnf-alpha), interleukin; 1-be ... | 1991 | 1918723 |
| two screening methods for detection of specific ige to inhalant allergens. comparison with skin prick test and rast. | we analyzed the technical features of two new screening methods for detection of specific ige to inhalant allergens. moreover, their diagnostic accuracy was evaluated comparing the results obtained from the procedures with those from rast and skin prick test (spt) in a group of 30 symptomatic allergic patients. the procedures employ a classic elisa method, where the presence of ige is revealed by a colored reaction. these two assays appeared to be quick, inexpensive and easy to use. the results ... | 1991 | 1669590 |
| high frequency of il-4-producing cd4+ allergen-specific t lymphocytes in atopic dermatitis lesional skin. | in atopic dermatitis (ad) hypersensitivity reactions to allergens are commonly observed and are assumed to make a major contribution in the pathomechanism of the disease. it may be expected that allergen-reactive th cells play a central role in these reactions. in the present study the occurrence and function of allergen-specific t lymphocytes in dermal inflammatory lesions were studied. to this aim panels of randomly cloned cd4+ t cells from lesional skin biopsies of two housedust mite dermatop ... | 1991 | 1714926 |
| newly generated ige antibodies to dermatophagoides pteronyssinus in children are directed against components distinct from der p i and der p ii. | the specificity of newly generated ige antibodies (abs) to the house dust mite, dermatophagoides pteronyssinus, in longitudinal serum samples from 18 young children with an increased risk for ige-mediated allergy was studied. the first ige ab response to house dust mite was detected early in life (mean age, 32 months; range, 11 to 60 months). for 83% of the children, more than half of the newly generated ige ab response to house dust mite was directed against components distinct from the major a ... | 1991 | 1880326 |
| immunoblot multi-allergen inhibition studies of allergenic cross-reactivity of the dust mites lepidoglyphus destructor and dermatophagoides pteronyssinus. | the allergenic similarity of the pyroglyphid mite d. pteronyssinus and the glycyphagid mite l. destructor was investigated with a new immunoblotting inhibition technique allowing simultaneous comparison of several allergens. extracts of d. pteronyssinus and l. destructor were separated by sds-page and electroblotted to nitrocellulose (nc). a serum pool containing ige specific to the major allergens in both mites was mixed with serially diluted extracts of d. pteronyssinus and l. destructor and i ... | 1991 | 1913274 |
| dermatophagoides pteronyssinus specific igm antibody and its complement activation in children with bronchial asthma. | western blotting analysis and elisa were used to examine dp specific igm abs in the sera and sputa of bronchial asthmatic and non-atopic children. with western blotting dp specific igm abs were detected binding diffusely to high molecular weight (greater than 65 kd) components of dp extracts and from the intensities of the staining of the sera the asthmatic children were divided into two groups: an intensive staining and a faint staining group. the non-atopic children showed still fainter staini ... | 1991 | 1892439 |
| house dust mite-derived amylase: allergenicity and physicochemical characterization. | amylase activity was found in extracts of both dermatophagoides pteronyssinus whole mite (0.16 u/mg) and spent growth medium (0.01 u/mg) but not in unused growth medium. it was also detected in all extracts of house dust obtained from mattresses (n = 20; geometric mean, 1.95 u/gm) and in 18 extracts of dust obtained from lounge room carpets (n = 20; geometric mean, 0.54 u/gm). although the origins of amylase in dust are unclear, enzyme activity correlated with mite counts (n = 40; r = 0.35; p le ... | 1991 | 1710630 |
| changes in the levels of house dust mite specific igg4 during immunotherapy in asthmatic children. | serum levels of igg subclass and house dust mite (dermatophagoides pteronyssinus, dpt) specific igg4 were evaluated during immunotherapy in asthmatic children. asthmatic children undergoing long-term immunotherapy (more than 2 years) posed a mean value of total serum igg4 or dpt-specific igg4 antibodies significantly higher than that of patients prior to receiving immunotherapy, asthmatic (placebo) controls, or patients undergoing short-term immunotherapy (less than 1 year) (p less than 0.05). t ... | 1991 | 1863900 |
| antiinflammatory-antioxidant treatment with a methane sulfonanilide in allergen-induced asthma. | two groups of six asthmatic patients with biphasic bronchospastic response to inhaled dermatophagoides pteronyssinus allergen extract were studied in a double-blind fashion. early and late asthmatic reactions to allergen inhalation challenge were determined before and at the end of a 2-week treatment period with nimesulide (100 mg bid orally), a sulfonanilide with antioxidant properties, or placebo. bronchial responsiveness to methacholine was evaluated 24 hours before and after allergen inhalat ... | 1991 | 1852141 |
| effect of h1-receptor blockade on late cutaneous reactions to antigen: a double-blind, controlled study. | this study is of the effect of the blockade of histamine h1 receptors by a long-acting antihistamine on the immediate and late clinical response to antigen (ag) and on the recruitment of eosinophils in the late-phase cutaneous reaction. ten adult volunteers with late-phase reactions to the intradermal injection of either dermatophagoides pteronyssinus or phleum pratense (timothy) pollen performed a double-blind, crossover study. each volunteer took astemizole, 10 mg, or identical placebo, daily ... | 1991 | 1673976 |
| [accelerated desensitization to dermatophagoides pteronyssinus mites in children with asthma. results of two years of immunotherapy]. | 16 children with chronic asthma were given a rush immunotherapy with standardized dermatophagoides pteronyssinus extracts. for two years, the dose of 10 ir was injected monthly. before this rush, six children have received a typical immunotherapy ineffective on asthma. examination of clinical, medication, immuno-allergologic and functional parameters were recorded before and at 6, 12 and 24 months of the follow-up. rush immunotherapy was well tolerated. substantial improvement of asthmatic syndr ... | 1991 | 2069687 |
| theophylline inhibits early and late asthmatic reactions induced by allergens in asthmatic subjects. | to determine whether oral slow-release theophylline inhibits asthmatic reactions and the associated increase of airway responsiveness to methacholine induced by allergens, we examined six asthmatic subjects who developed a dual asthmatic reactions after allergen bronchoprovocation with dermatophagoides pteronyssinus or with grass pollen. we gave oral slow-release theophylline and placebo to each subject for seven days in two series of experiments in a double-blind, randomized, crossover study. t ... | 1991 | 2006773 |
| number of beta-receptors in patients allergic to dermatophagoides pteronyssinus. | this study was carried out on 96 atopic patients with monosensitization against dermatophagoides pteronyssinus and 30 control individuals. the patients were divided into 3 subgroups: 27 asymptomatic patients, 27 patients who only presented nasal symptomatology, and 27 patients with marked bronchial symptomatology. total serum ige, antigen-specific histamine release, and the number of beta 2-adrenergic receptors in peripheral blood lymphocytes in all patients were determined. the control group pr ... | 1991 | 1727007 |
| comparative study between two immunoassays for detection of allergen-specific igg4. | in the present work two commercially available immunoassays for allergen-specific igg4 detection were compared, igg4 fast test and allerblock-ms igg4 eia kit. for this purpose 30 serum samples were tested for igg4 antibodies (abs) specific to dermatophagoides pteronyssinus (dp) by the two immunoassays. eleven serum samples were collected from normal subjects, 6 from dp-atopic patients never treated by specific immunotherapy (its) and 13 from dp-atopic patients previously treated by its. pearson' ... | 1991 | 1669562 |
| injection of allergen-antibody complexes is an effective treatment of atopic dermatitis. | atopic dermatitis (ad) can be exacerbated by contact with airborne allergens, amongst which dermatophagoides pteronyssinus (dpt) appears to be potentially important. specific ige antibodies towards dpt are often found in ad, and it can therefore be speculated that suppression of the production of anti-dpt ige might result in a significant clinical improvement. complexes of antigen and specific antibodies have been shown to suppress the production of antibody in other systems; we report here the ... | 1991 | 2050242 |
| short-term effect of solidified benzyl benzoate on mite-allergen concentrations in house dust. | the effect of solidified benzyl benzoate (bb) foam and powder on concentrations of the major allergens of dermatophagoides pteronyssinus (der p i) and d. farinae (der f i) in dust samples from mattresses and carpets in 22 households was investigated in a randomized, double-blind, placebo-controlled trial. all houses had been demonstrated to have significant (greater than or equal to 2000 ng/gm of dust) mite-allergen concentrations on mattresses. dust samples were collected from mattresses and ca ... | 1991 | 1991922 |
| investigation of house dust mite (dermatophagoides pteronyssinus) allergen extracts prepared from purified mite bodies and whole mite culture. | allergen extracts prepared from purified mite bodies (pmb) and whole mite culture (wmc) were investigated by using in vivo and in vitro methods. pmb showed a more complex allergen pattern than wmc. in pmb, 6 out of 16 allergens could be detected as major allergens. in wmc, 5 out of 10 allergens could be detected as major allergens. both extracts contained the same amount of the major allergen der p i. by skin prick testing comparable results were obtained with both extracts. investigation of the ... | 1991 | 1937904 |
| tribolium confusum (confused flour beetle, rice flour beetle)--an occupational allergen in bakers: demonstration of ige antibodies. | specific ige to proteins from tribolium confusum (tc), a flour beetle, was detected in 9/125 sera of subjects exposed to rye and wheat flour. tc rast was not inhibited by dermatophagoides pteronyssinus, rye or wheat flour. immunoblot experiments showed specific binding to three proteins from adult tc or pupae, not present in rye or wheat flour. these findings suggest that tc might act as an occupational allergen in a proportion of bakers. | 1991 | 1937903 |
| ultrastructural localization of the allergen der p i in the gut of the house dust mite dermatophagoides pteronyssinus. | the sites and concentrations of der p i within the house dust mite were determined. highest concentrations were found in the epithelium of the posterior end of the mite stomach, implying that this is the site of der p i synthesis and secretion. | 1991 | 1937901 |
| faecally derived hydrolytic enzymes from dermatophagoides pteronyssinus: physicochemical characterisation of potential allergens. | the previous findings that the group i and iii mite allergens, and amylase present in mite faeces are hydrolytic enzymes has prompted a study to determine whether this material contains other enzymes which could be allergenic. thus, spent growth medium devoid of whole dermatophagoides pteronyssinus mites was shown to contain glucoamylase, lipase and lysozyme in addition to the cysteine protease, serine protease and amylase activities associated with the above allergens, respectively. all of thes ... | 1991 | 1718911 |
| allergenicity and physicochemical characterization of house dust mite derived amylase. | the enzyme amylase was shown to be present in extracts prepared from both house dust and spent growth medium used in the culture of the mite dermatophagoides pteronyssinus. in dust, it was shown to correlate with both mite counts and concentrations of the faecally derived mite allergen, der p i. mite amylase was isolated from the culture medium and shown to be a single chain protein with a molecular weight of 56,000. the enzyme contained free sulphydryl groups and had the n-terminal sequence, ky ... | 1991 | 1718897 |
| cytokine production by allergen (der pi)-specific cd4+ t cell clones derived from a patient with severe atopic disease. | twenty-four t cell clones (tcc) specific for purified dermatophagoides pteronyssinus group i allergen (der p i) were established from the peripheral blood of a patient with severe atopic disease and assessed for cytokine production in response to stimulation with both phorbol 12-myristate 13-acetate and anti-cd3 monoclonal antibody. all der p i-specific tcc produced high amounts of interleukin (il)-4 in association with il-3, il-5 and granulocyte monocyte-colony stimulating factor (gm-csf), wher ... | 1991 | 1687726 |
| patch tests with house dust mite antigens in atopic dermatitis patients: methodological problems. | patch tests with house dust mite allergens were performed in 21 atopic dermatitis patients with a positive prick test and rast for house dust mite. variables in methodology of patch testing, i.e. allergen concentration, application time, and intensity of tape stripping, were studied. tests were performed with dermatophagoides pteronyssinus solutions containing 20x, 100x and 500x the prick test concentration and purified hdm antigen: 10 and 50 micrograms/ml p1ag solution. the series was applied o ... | 1991 | 1681645 |
| atopic dermatitis: recent therapeutic advances. | new treatments were recently proposed for the management of severe atopic dermatitis (ad). they all act on some component of the immune reaction. oral cyclosporine reduces the number of cd4+ cells, the secretion of interleukins, and the function of langerhans cells. although the action of oral cyclosporine at moderately high dosages is regular and rapid, the risk of serious side effects and the reappearance of progressive disease after stopping treatment limits the use of this drug in ad. thymic ... | 1992 | 1492066 |
| molecular polymorphisms of house dust mite allergens. | previous studies from this laboratory have described the primary amino acid sequences of the group i and group ii allergens from dermatophagoides pteronyssinus and d. farinae. this report concentrates on polymorphisms of allergens within a species. firstly, four cdna clones of der fii produced by polymerase chain reaction have been sequenced and are compared to the sequences published previously by ourselves and others. although the sequences come from different sources, australia and japan, the ... | 1992 | 1493745 |
| measurement of igg subclass antibodies to the group ii antigen of dermatophagoides pteronyssinus (der p ii) in sera from children with bronchial asthma. | seven igg-binding components in crude mite allergen extracted from dermatophagoides pteronyssinus (der p) were identified by immunoblotting analysis. of these components, the 15-kda protein was strongly recognized by igg from patients with bronchial asthma, whereas it was less recognized by igg from healthy individuals. then, the 15-kda protein was purified by reverse-phase high performance liquid chromatography and the protein was demonstrated to induce immediate positive skin reactions in pati ... | 1992 | 1456484 |
| early immune responses to dermatophagoides pteronyssinus and atopic predisposition. | responses to the house dust mite during infancy may be important determinants of asthma in susceptible individuals. this study assessed early igg subclass antibody responses to dermatophagoides pteronyssinus in children of atopic parents. sixteen atopic and 15 non-atopic children were selected from a birth cohort, and atopic status was established according to follow up over the first two years. igg1 and igg4 antibodies to d pteronyssinus were measured by enzyme linked immunosorbent assay at 7 d ... | 1992 | 1520005 |
| effect of astemizole on allergic asthma. | in a placebo-controlled, double-blind, crossover study with a washout phase of 2 months between each treatment period, single daily doses of 10 and 30 mg of astemizole were given to 12 patients with extrinsic asthma during 28 consecutive days. albuterol was allowed as concurrent medication when needed. on days 0, 7, and 28 specific bronchial provocation with dermatophagoides pteronyssinus and cutaneous tests with histamine and d. pteronyssinus extracts were carried out. the requirements of beta- ... | 1992 | 1510286 |
| effect of allergen avoidance on development of allergic disorders in infancy. | there is much evidence that the development of allergic disorders may be related to early exposure of allergens, including those in breastmilk. we have tried to find out whether avoidance of food and inhaled allergens in infancy protects against the development of allergic disorders in high-risk infants. in a prenatally randomised, controlled study 120 infants with family history of atopy and high (greater than 0.5 ku/l) cord-blood concentrations of total ige were allocated randomly to prophylac ... | 1992 | 1351183 |
| localization of the major house dust mite allergen der p i in the body of dermatophagoides pteronyssinus by immustain. | 1992 | 1393762 | |
| isolation of der pi, the dermatophagoides pteronyssinus major mite allergen, from a crude mite culture extract, purification by ion-chromatography, and comparison between the material obtained and a cdna-coded der pi. | a high degree of purity is a prerequisite for an allergen preparation to be suitable for clinical diagnosis and therapy. a pure allergen can easily be obtained from a crude mite culture extract by using an immunosorbent prepared with highly specific monoclonal antibodies or from a cdna-coded material. however, up to now none of these methods has been performed on a process scale. here large-scale purification is defined as a process in which a crude dermatophagoides pteronyssinus mite culture ex ... | 1992 | 1618983 |
| t cell responses to the purified major allergens from the house dust mite dermatophagoides pteronyssinus. | proliferation assays were used to determine peripheral blood t cell responses to affinity-purified der p i, der p ii, and der f ii allergens. patients studied were sensitive to the house dust mite (hdm) either alone or in combination with other allergens. control subjects were both nonatopic and atopic non-hdm sensitive. in general, only hdm-sensitive patients responded to the mite allergens. mean stimulation indices (si) were 10.2 +/- 2.8 (sem) for der p i and 10.0 +/- 2.2 for der p ii in hdm-s ... | 1992 | 1583244 |
| a double-blind controlled trial of hyposensitization to dermatophagoides pteronyssinus in children with atopic eczema. | a double-blind controlled trial of hyposensitization with tyrosine-adsorbed dermatophagoides pteronyssinus vaccine in 24 children with atopic eczema and immediate hypersensitivity to d. pteronyssinus failed to demonstrate superiority over placebo after a standard 8 month course of treatment. in a second phase, children initially administered active treatment were randomly allocated to continue with active treatment or switched to placebo for a further 6 months. the clinical scores suggest that p ... | 1992 | 1611544 |
| ige, igg, and igg4 antibody titers to fractionated house dust mite antigens in nasal allergy patients. | using 6 fractions differing in molecular weight of dermatophagoides pteronyssinus (dp)-antigen, we measured by enzyme-linked immunosorbent assay (elisa) the titers of specific ige, igg and igg4 antibodies against dp antigen in sera of allergic subjects who were sensitive to house dust mite. we intended to evaluate which dp fraction acts as the major antigenicity for allergic subjects. results were as follows: 1) in comparison with normal controls, the titer of ige antibody specific to crude dp a ... | 1992 | 1575063 |
| effect of hyposensitization upon the immediate and late asthmatic reaction and upon histamine reactivity in patients allergic to house dust mite (dermatophagoides pteronyssinus). | the effect of hyposensitization (hs) upon the allergen evoked immediate asthmatic reaction (iar), late asthmatic reaction (lar) and upon bronchial reactivity towards histamine was examined. eighteen young asthmatic patients were studied using a double-blind, placebo-controlled design. all were allergic to house dust mite (hdm) and demonstrated an iar and a lar after bronchial provocation with hdm. furthermore, all, except one child, demonstrated bronchial hyperreactivity towards histamine (media ... | 1992 | 1572445 |
| laser skin testing. | in order to accomplish more uniformity for standardization of allergy skin testing, a technique using a co2 laser in place of a needle was developed. the dose of laser light was based on comparison of biopsies of punctures with morrow-brown needles and varying combinations of laser energy parameters. the resulting laser settings were tested in twenty volunteers who had been referred for skin testing. blinded tests were conducted with both procedures in parallel using histamine, saline, dermatoph ... | 1992 | 1546824 |
| a comparative study of allergenic and potentially allergenic enzymes from dermatophagoides pteronyssinus, d. farinae and euroglyphus maynei. | the presence of the enzymatically active allergens equivalent to der p i (cysteine protease), der p iii (serine protease) and amylase in extracts of dermatophagoides pteronyssinus, d. farinae and euroglyphus maynei was determined using appropriate enzymatic techniques. biochemical equivalents of all three allergens were present in each extract studied. studies also showed that the mite extracts contained a variety of other biochemically active enzymes including trypsin, chymotrypsin, carboxypept ... | 1992 | 1283568 |
| combination of patch test and ige for dust mite antigens differentiates 130 patients with atopic dermatitis into four groups. | patients with atopic dermatitis sometimes have positive responses to patch testing (pt) with dust mite antigens, which is believed to correlate with the elevated levels of specific ige for those antigens. | 1992 | 1401304 |
| in vitro activation of bronchoalveolar lavage cells by house dust mite allergens. | mast cells represent a small but important proportion of bronchoalveolar lavage cells and are directly exposed to environmental triggers including allergens. histamine and pgd2 are mediators released during the activation of mast cells. fourteen patients allergic to dermatophagoides pteronyssinus were studied. after bronchoalveolar lavage the unfractionated cell pellet containing metachromatic cells was submitted to allergen challenge using three concentrations of a standardized dermatophagoides ... | 1992 | 1371209 |
| frequency analysis of allergen-reactive t-lymphocytes in individuals allergic against the house-dust mite dermatophagoides pteronyssinus. | we have investigated the frequencies of dermatophagoides pteronyssinus (dpt)-reactive circulating t cells in individuals allergic to this house dust mite and in asymptomatic controls. in allergic patients the median for frequencies of dpt-reactive t cells (80/10,000 t cells) was significantly higher (p = 0.0001) than that in the controls (19/10,000 t cells). frequencies of spontaneously proliferating t cells were also determined. here too, marked differences (p = 0.0001) were observed between th ... | 1992 | 1624207 |
| [role of the acarid euroglyphus maynei in allergic respiratory diseases in children]. | the author has search specific ige antibodies anti-euroglyphus maynei house dust mite among 200 children and teenagers. all had respiratory disease with a presumed allergic etiology. specific ige were found in eighty three subjects, all of them with hypersensitivity against dermatophagoïdes pteronyssinus house dust mite. none parameter can explain this hypersensitivity but these hypersensitive subjects are older than others. | 1992 | 1606512 |
| characterization of ige antibody binding profiles of sera from patients with atopic dermatitis to allergens of the domestic mites dermatophagoides pteronyssinus and euroglyphus maynei, using enhanced chemiluminescent immunoblotting. | a rapid, sensitive, chemiluminescent immunoblotting method was used to identify ige-binding components of dermatophagoides pteronyssinus and euroglyphus maynei that were recognized by sera of 16 d. pteronyssinus-sensitive patients with atopic dermatitis. some 28 components (14-200 kd) were found in the d. pteronyssinus extract, and sera bound between 1 and 13 of them (mean 6.0). at least one of four components (97, 28, 24 and 14 kd) were bound by 14/16 sera. e. maynei had 25 components (14-227 k ... | 1992 | 1582697 |
| bronchial and bronchoalveolar lavage after allergen challenge in atopic patients. | twenty patients with allergic rhinitis and/or asthma sensitised to dermatophagoides pteronyssinus were studied. on two consecutive days they underwent methacholine challenge and allergen bronchial challenge. 72 h after allergen challenge, fibreoptic bronchoscopy with bronchial (bl) and bronchoalveolar (bal) lavage was performed. specific ige antibodies were measured both in serum and in bl from 14 patients. both bal and bl cell differentials were similar in patients with isolated early response ... | 1992 | 1579739 |
| the igg subclass antibody response to an inhalant antigen (dermatophagoides pteronyssinus) during the first year of life: evidence for early stimulation of the immune system following natural exposure. | this study investigates the early humoral immune response following natural exposure to an inhalant antigen (dermatophagoides pteronyssinus) in 36 babies, from birth until 1 year of age. the total igg and subclass 1 and 4 d. pteronyssinus-specific antibody levels were assayed in sera collected at 7 days, 3 and 12 months by elisa. after an initial fall, due to the progressive loss of maternal antibodies, an igg specific response to d. pteronyssinus was seen between 3 and 12 months. this was restr ... | 1992 | 1551032 |
| the group iii allergen from the house dust mite dermatophagoides pteronyssinus is a trypsin-like enzyme. | faecally enriched extracts of dermatophagoides pteronyssinus were shown to contain a trypsin-like enzyme which was allergenic. chromatofocusing studies revealed the presence of nine major isoforms in d. pteronyssinus, with pi in the range 4 to greater than 8, but only two (range 4-5) in d. farinae. trypsin isolated from d. pteronyssinus by benzamidine-sepharose 6b affinity chromatography and gelfiltration was found to be a 31-kda protein which was enzymatically similar to both invertebrate and v ... | 1992 | 1537598 |
| treatment of atopic dermatitis by allergen-antibody complexes: long-term clinical results and evolution of ige antibodies. | this study describes the long-term follow-up of the clinical response of 10 adult patients suffering from atopic dermatitis (ad) who were treated by administration of complexes made of dermatophagoides pteronyssinus (dpt) allergens and autologous antibodies specific to that allergen. we have already described the clinical improvement observed after 1 year of treatment involving regular injections of complexes; this improvement was maintained throughout a second year, even though the number of in ... | 1992 | 1498393 |
| molecular characterisation of group i allergen eur m i from house dust mite euroglyphus maynei. | using the polymerase chain reaction (pcr) we have amplified and cloned genomic dna encoding the secreted group i allergen proteins from the house dust mite species euroglyphus maynei, dermatophagoides pteronyssinus and d. farinae. affinity chromatography using a monoclonal antibody to the allergen der p i was used to purify the group i protein from e. maynei. we present the deduced amino acid sequence of a new member of the group i house dust mite allergen family eur m i. the three proteins show ... | 1992 | 1483062 |
| the effect of dermatophagoides pteronyssinus allergens on proliferation and cd23 antigen expression of peripheral blood lymphocytes from atopic patients. | the low affinity ige receptor (fc epsilon rii/cd23) has been proposed to be involved in the regulation of ige synthesis. the present study was undertaken to investigate the responses to in vitro stimulation by allergen (dermatophagoides pteronyssinus; d.p) and/or interleukin-4 (il-4) of peripheral blood lymphocytes (pbls) isolated from atopic and non-atopic subjects. il-4 induced up to 5 fold increase in cd23 expression on pbls from both atopic patients and normal controls, whereas the d.p extra ... | 1992 | 1477031 |
| associations between atopy, asthma history, respiratory function and non-specific bronchial hyperresponsiveness in unselected young asthmatics. | we evaluated the relationship between bronchial hyperresponsiveness and atopic status, clinical symptoms and airway calibre in a group of italian conscripts who reported having bronchial asthma. 126 subjects were studied. bronchial responsiveness was measured by the methacholine test, and atopic status was assessed by skin tests. a measurable pc20 was detected in 106 subjects. on the basis of the methacholine threshold concentration the sample was divided into four categories. subjects with a lo ... | 1992 | 1439229 |
| house-dust mite allergy and eosinophilia in patients with asthma in rangoon (yangon). | skin tests were done by prick and intradermal techniques, using house dust mite (dermatophagoides pteronyssinus) antigen on 35 patients with early onset asthma, 33 with late onset asthma, 43 with asthma and frequent cough with sputum production (chronic bronchitis) and 30 control subjects. absolute blood eosinophil and sputum eosinophil (as percentage of leukocytes) counts were performed on each patient. positive skin tests to house dust mite antigens were significantly more frequent in each of ... | 1992 | 1429392 |