Publications

complete mitochondrial genome of the asian paddle crab charybdis japonica (crustacea: decapoda: portunidae): gene rearrangement of the marine brachyurans and phylogenetic considerations of the decapods.	given the commercial and ecological importance of the asian paddle crab, charybdis japonica, there is a clearly need for genetic and molecular research on this species. here, we present the complete mitochondrial genome sequence of c. japonica, determined by the long-polymerase chain reaction and primer walking sequencing method. the entire genome is 15,738 bp in length, encoding a standard set of 13 protein-coding genes, two ribosomal rna genes, and 22 transfer rna genes, plus the putative cont ...	2010	19714481
anatomy of virgin and mature externae of loxothylacus texanus, parasitic on the dark blue crab callinectes rathbunae (crustacea: cirripedia: rhizocephala: sacculinidae).	rhizocephalan parasites are dioecious organisms, in that one or several dwarf males are implanted into the external part of the female parasite soon after it emerges from the interior of the host animal. the structure of the female externa and its resident males is crucial for understanding both the reproductive biology and the taxonomy of these specialized parasites. we use scanning electron microscopy and histological methods to study the anatomy of juvenile and the mature externae of the rhiz ...	2010	19714752
bioaccumulation and depuration of metals in blue crabs (callinectes sapidus rathbun) from a contaminated and clean estuary.	blue crabs from a contaminated estuary (hackensack meadowlands-hm) and a cleaner reference site (tuckerton-tk) were analyzed for cr, cu, hg, pb, and zn in muscle and hepatopancreas. crabs from each site were taken into the laboratory and fed food from the other site, or in another experiment, transplanted to the other site for eight weeks. all crabs were analyzed for metals. overall, tissue concentrations reflected environmental conditions. tissue differences were found for cu, pb and zn (all hi ...	2010	19783083
feedback from peripheral musculature to central pattern generator in the neurogenic heart of the crab callinectes sapidus: role of mechanosensitive dendrites.	the neurogenic heart of decapod crustaceans is a very simple, self-contained, model central pattern generator (cpg)-effector system. the cpg, the nine-neuron cardiac ganglion (cg), is embedded in the myocardium itself; it generates bursts of spikes that are transmitted by the cg's five motor neurons to the periphery of the system, the myocardium, to produce its contractions. considerable evidence suggests that a cpg-peripheral loop is completed by a return feedback pathway through which the cont ...	2010	19828726
hemolymph ecdysteroids during the last three molt cycles of the blue crab, callinectes sapidus: quantitative and qualitative analyses and regulation.	the profiles of circulating ecdysteroids during the three molt cycles prior to adulthood were monitored from the juvenile blue crab, callinectes sapidus. ecdysteroid patterns are remarkably similar in terms of peak concentrations ranging between 210-330 ng/ml hemolymph. analysis of hemolymph at late premolt stage revealed six different types of ecdysteroids with ponasterone a (poa) and 20-oh ecdysone (20-oh e) as the major forms. this ecdysteroid profile was consistent in all three molt cycles. ...	2010	19557853
bacterial diseases of crabs: a review.	bacterial diseases of crabs are manifested as bacteremias caused by organisms such as vibrio, aeromonas, and a rhodobacteriales-like organism or tissue and organ tropic organisms such as chitinoclastic bacteria, rickettsia intracellular organisms, chlamydia-like organism, and spiroplasma. this paper provides general information about bacterial diseases of both marine and freshwater crabs. some bacteria pathogens such as vibrio cholerae and vibrio vulnificus occur commonly in blue crab haemolymph ...	2011	21215353
molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus trituberculatus.	an extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ...	2011	20848212
mrna expression and activity of ion-transporting proteins in gills of the blue crab callinectes sapidus: effects of waterborne copper.	waterborne cu effects on the transcription of genes encoding ion-transporting proteins and the activities of these proteins were evaluated in gills of the blue crab callinectes sapidus acclimated to diluted (2‰) and full (30‰) seawater. crabs were exposed (96 h) to an environmentally relevant concentration of dissolved cu (0.78 µm) and had their posterior (osmoregulating) gills dissected for enzymatic and molecular analysis. endpoints analyzed were the activity of key enzymes involved in crab os ...	2011	20928920
changes in intracellular calcium concentration in crustacean (callinectes sapidus) y-organs: relation to the hemolymphatic ecdysteroid titer.	secretion of ecdysteroid molting hormones by crustacean y-organs is negatively regulated (inhibited) by molt-inhibiting hormone (mih), a neuropeptide produced by neurosecretory cells in eyestalk ganglia. the inhibitory effect of mih is mediated by one or more cyclic nucleotide second messengers. in addition, available data indicate that ecdysteroidogenesis is positively regulated (stimulated) by intracellular calcium. however, despite the apparent critical role of calcium in regulating ecdystero ...	2011	20938968
water quality trends in the delaware river basin (usa) from 1980 to 2005.	in 1940, the tidal delaware river was "one of the most grossly polluted areas in the united states." during the 1950s, water quality was so poor along the river at philadelphia that zero oxygen levels prevented migration of american shad leading to near extirpation of the species. since then, water quality in the delaware basin has improved with implementation of the 1961 delaware river basin compact and 1970s federal clean water act amendments. at 15 gages along the delaware river and major tri ...	2011	20665109
comparative distribution, sourcing, and chemical behavior of pcdd/fs and pcbs in an estuary environment.	pcdd/f and pcb field data (1041 samples) in five media (dissolved, suspended sediment, bed sediment, catfish, and blue crab) were studied to explore dual contaminant patterns in the houston ship channel, texas, usa. pcdd/fs showed greater concentration than pcbs in suspended sediments while pcbs were higher in apparent dissolved (truly dissolved+doc-associated), fish, and crab. pcdd/fs at nearly all locations contributed more strongly to dioxin-like toxicity. the fraction of pcb teq was, however ...	2011	21440284
radiation inactivation of foodborne pathogens on frozen seafood products.	foodborne illness due to consumption of contaminated seafood is, unfortunately, a regular occurrence in the united states. ionizing (gamma) radiation can effectively inactivate microorganisms and extend the shelf life of seafood. in this study, the ability of gamma irradiation to inactivate foodborne pathogens surface inoculated onto frozen seafood (scallops, lobster meat, blue crab, swordfish, octopus, and squid) was investigated. the radiation d(10)-values (the radiation dose needed to inactiv ...	2011	21477481
getting ahead: context-dependent responses to odorant filaments drive along-stream progress during odor tracking in blue crabs.	the chemosensory signal structure governing the upstream progress of blue crabs to an odorant source was examined. we used a three-dimensional laser-induced fluorescence system to collect chemical concentration data simultaneously with behavior observations of actively tracking blue crabs (callinectes sapidus) in a variety of plume types. this allowed us to directly link chemical signal properties at the antennules and legs to subsequent upstream motion while altering the spatial and temporal in ...	2011	21490258
staying the course: chemical signal spatial properties and concentration mediate cross-stream motion in turbulent plumes.	this study examined the role of broadly distributed sensor populations in chemosensory searching, especially cross-stream heading adjustment. we used three-dimensional laser-induced fluorescence to collect chemical concentration data simultaneously with behavior observations of actively tracking blue crabs (callinectes sapidus). our analysis indicates that the spatial distribution of the odorant concentration field is necessary and sufficient to mediate correct cross-stream motion, although conc ...	2011	21490259
evaluation of potential relationships between chemical contaminants in sediments and aquatic organisms from the lower passaic river, new jersey, usa.	the lower passaic river, new jersey, usa, a tidal tributary to newark bay and part of the new york and new jersey harbor estuary, is contaminated with a variety of persistent organic pollutants (pops) due to nearly two centuries of heavy industrialization and urbanization. resident aquatic organisms are exposed to, and can bioaccumulate, a variety of chemical contaminants from sediments, water and other organisms. in the present study, the relationships between selected pops detected in both sur ...	2011	21520249
cloning of an insulin-like androgenic gland factor (iag) from the blue crab, callinectes sapidus: implications for eyestalk regulation of iag expression.	in malacostracan crustaceans, sex differentiation is uniquely regulated by a hormone secreted by the male-specific androgenic gland (ag). an isopod ag hormone was the first to be structurally elucidated and was found to belong to the insulin superfamily of proteins. recently, it has been found that the ags of several decapod crustaceans express insulin-like androgenic gland factors (iags), whose function is believed to be similar to that of the isopod ag hormone. here we report the isolation fro ...	2011	21596044
prophenoloxidase activating enzyme-iii from giant freshwater prawn macrobrachium rosenbergii: characterization, expression and specific enzyme activity.	the prophenoloxidase activating system is an important innate immune response against microbial infections in invertebrates. the major enzyme, phenoloxidase, is synthesized as an inactive precursor and its activation to an active enzyme is mediated by a cascade of clip domain serine proteinases. in this study, a cdna encoding a prophenoloxidase activating enzyme-iii from the giant freshwater prawn macrobrachium rosenbergii, designated as mrproae-iii, was identified and characterized. the full-le ...	2011	21614523
the role of alternate hosts in the ecology and life history of hematodinium sp., a parasitic dinoflagellate of the blue crab (callinectes sapidus).	abstract abstract: hematodinium sp. infections are relatively common in some american blue crab (callinectes sapidus) populations in estuaries of the western atlantic ocean. outbreaks of disease caused by hematodinium sp. can be extensive and can cause substantial mortalities in blue crab populations in high salinities. we examined several species of crustaceans to determine if the same species of hematodinium that infects c. sapidus is found in other crustaceans from the same localities. over a ...	2011	21812642
revisiting the reticulum: feedforward and feedback contributions to motor program parameters in the crab cardiac ganglion microcircuit.	the neurogenic heartbeat of crustaceans is controlled by the cardiac ganglion (cg), a central pattern generator (cpg) microcircuit composed of nine neurons. in most decapods, five 'large' motor neurons (mns) project from the cg to the myocardium, where their excitatory synaptic signals generate the rhythmic heartbeat. the processes of four 'small' premotor neurons (pmns) are confined to the cg, where they provide excitatory drive to the mns via impulse-mediated chemical signals and electrotonic ...	2011	21775716
comparison of metal concentrations in tissues of blue crab, callinectes sapidus from mediterranean lagoons.	this study was performed to investigate the metal concentrations in muscle and gill of blue crab, callinectes sapidus from dörtyol lake, akyatan lagoon, paradeniz lagoon and çamlik lagoon from the northeastern coastal area of mediterranean sea. so, the levels of cadmium, chromium, copper, iron, manganese, nickel, aluminum and zinc in tissues of specimens from the lagoons were determined by inductively coupled plasma optical emission spectrometer. the metal concentrations found in muscle tissue v ...	2011	21755288
discovery and characterization of the crustacean hyperglycemic hormone precursor related peptides (cprp) and orcokinin neuropeptides in the sinus glands of the blue crab callinectes sapidus using multiple tandem mass spectrometry techniques.	the crustacean sinus gland (sg) is a well-defined neuroendocrine site that produces numerous hemolymph-borne agents including the most complex class of endocrine signaling molecules-neuropeptides. via a multifaceted mass spectrometry (ms) approach, 70 neuropeptides were identified including orcokinins, orcomyotropin, crustacean hyperglycemic hormone (chh) precursor-related peptides (cprps), red pigment concentrating hormone (rpch), pigment dispersing hormone (pdh), proctolin, rfamides, ryamides, ...	2011	21740068
influence of diffuse and chronic metal pollution in water and sediments on edible seafoods within ondo oil-polluted coastal region, nigeria.	the bioconcentration levels of 3 non-essential elements (pb, cd and ni) have been investigated in three different seafoods; fish (tilapia zilli), crab (callinectes sapidus) and periwinkle (littorina littorea), to investigate the ecosystem health status in ondo oil-polluted coastal region, nigeria. the seafood samples were chosen based on their popularity as a food source and the potential of the species to contain high levels of metals based on past research results. metal concentrations in the ...	2011	21714630
Membrane-assisted capillary isoelectric focusing coupling with matrix-assisted laser desorption/ionization-Fourier transform mass spectrometry for neuropeptide analysis.	Herein we report a highly efficient and reliable membrane-assisted capillary isoelectric focusing (MA-CIEF) system being coupled with MALDI-FTMS for the analysis of complex neuropeptide mixtures. The new interface consists of two membrane-coated joints made near each end of the capillary for applying high voltage, while the capillary ends were placed in the two reservoirs which were filled with anolyte (acid) and catholyte (base) to provide pH difference. Optimizations of CIEF conditions and com ...	2011	21696746
The spatial and temporal patterns of odors sampled by lobsters and crabs in a turbulent plume.	Odors are dispersed across aquatic habitats by turbulent water flow as filamentous, intermittent plumes. Many crustaceans sniff (take discrete samples of ambient water and the odors it carries) by flicking their olfactory antennules. We used planar laser-induced fluorescence to investigate how flicking antennules of different morphologies (long antennules of spiny lobsters, Panulirus argus; short antennules of blue crabs, Callinectes sapidus) sample fluctuating odor signals at different position ...	2011	21865526
Comparison of automated BAX PCR and standard culture methods for detection of Listeria monocytogenes in blue Crabmeat (Callinectus sapidus) and blue crab processing plants.	This study compared the automated BAX PCR with the standard culture method (SCM) to detect Listeria monocytogenes in blue crab processing plants. Raw crabs, crabmeat, and environmental sponge samples were collected monthly from seven processing plants during the plant operating season, May through November 2006. For detection of L. monocytogenes in raw crabs and crabmeat, enrichment was performed in Listeria enrichment broth, whereas for environmental samples, demi-Fraser broth was used, and th ...	2011	22054196
Morphological and Molecular Characterization of Hematodinium perezi (Dinophyceae: Syndiniales), a Dinoflagellate Parasite of the Harbour Crab, Liocarcinus depurator.	Hematodinium perezi Chatton and Poisson (Dinophyceae: Syndiniales) is reported from one of its type hosts, Liocarcinus depurator, from Rye Bay in the English Channel, a site in a similar geographical location to that of the type description. The histology and ultrastructure of vegetative trophont stages, and rDNA sequences of the parasite infecting this host are reported for the first time. Ultrastructurally, H. perezi was confirmed by the presence of condensed chromatin profiles, trichocysts, a ...	2011	22092696
effects of molting on the visual acuity of the blue crab, callinectes sapidus.	in crustaceans with compound eyes, the corneal lens of each facet is part of the exoskeleton and thus shed during molting. here we used an optomotor assay to evaluate the impact of molting on visual acuity (as measured by the minimum resolvable angle, α(min)) in the female blue crab, callinectes sapidus. we found that visual acuity decreases substantially in the days prior to molting and is gradually recovered after molting. four days prior to molting, α(min) was 1.8 deg (n=5), a value approxima ...	2011	21865518
properties of bacteria that trigger hemocytopenia in the atlantic blue crab, callinectes sapidus.	in the blue crab callinectes sapidus, injection with the bacterial pathogen vibrio campbellii causes a decrease in oxygen consumption. histological and physiological evidence suggests that the physical obstruction of hemolymph flow through the gill vasculature, caused by aggregations of bacteria and hemocytes, underlies the decrease in aerobic function associated with bacterial infection. we sought to elucidate the bacterial properties sufficient to induce a decrease in circulating hemocytes (he ...	2011	22042435
diet selectivity of juvenile blue crabs (callinectes sapidus) in chesapeake bay.	shallow coves in chesapeake bay have abundant food and serve as nursery grounds for juvenile blue crabs. in this study, we examined the relationships between the diet of very small (4-40 mm cw) juvenile blue crabs and the benthic infauna in shallow, unvegetated nursery coves. we compared infauna in benthic samples with gut contents of juvenile blue crabs from six shallow coves in each of two sub-estuaries (rappahannock and york rivers) in chesapeake bay, virginia, usa. benthic communities differ ...	2011	21859782
in vitro culture and developmental cycle of the parasitic dinoflagellate hematodinium sp. from the blue crab callinectes sapidus.	summaryhematodinium is a genus of parasitic dinoflagellates whose species have caused significant mortalities in marine crustacean fisheries worldwide. a species of hematodinium infects the blue crab, callinectes sapidus on the eastern seaboard of the usa. the mode of transmission of the parasite in blue crabs is unknown. we established several continuous in vitro cultures of hematodinium sp. isolated from the haemolymph of infected blue crabs. one isolate has been continuously maintained in our ...	2011	21902874
histopathological characterization and in situ detection of callinectes sapidus reovirus.	a reovirus (tentatively designated as callinectes sapidus reovirus, csrv) was found in the blue crabs c. sapidus collected in chesapeake bay in 2005. histological examination of hepatopancreas and gill from infected crabs revealed eosinophilic to basophilic, cytoplasmic, inclusions in hemocytes and in cells of connective tissue. a cdna library was constructed from total rna extracted from hemolymph of infected crabs. one clone (designated as csrv-28) with a 532-bp insert was 75% identical in nuc ...	2011	21925184
lack of transmission of hematodinium sp. in the blue crab callinectes sapidus through cannibalism.	hematodinium spp. are parasitic dinoflagellates of marine crustaceans. outbreaks of hematodinium sp. have impacted commercial landings of the blue crab callinectes sapidus in the coastal bays of virginia and maryland (usa), with seasonal peaks in prevalence reaching 85%. the life cycle and transmission routes of the parasite in blue crabs are poorly understood. cannibalism and waterborne transmission may be routes of transmission, although little conclusive evidence has been reported for these m ...	2011	22132503
a novel putative lipoprotein receptor (caslpr) in the hemocytes of the blue crab, callinectes sapidus: cloning and up-regulated expression after the injection of lps and lta.	the full-length cdna encoding a putative lipoprotein receptor (caslpr) was isolated from the hemocytes of callinectes sapidus using 5' and 3' races. the open reading frame for caslpr contains a precursor of putative caslpr consisting of 1710 amino acid residues including 22 amino acid residues of the signal peptide (22 amino acids). mature caslpr (1688 amino acids with 5.6% of phosphorylation sites) has multiple, putative functional domains: five low-density lipoprotein receptor domains in the n ...	2011	22155280
Na?,K?-ATPase activity in the posterior gills of the blue crab, Callinectes ornatus (Decapoda, Brachyura): modulation of ATP hydrolysis by the biogenic amines spermidine and spermine.	We investigated the effect of the exogenous polyamines spermine, spermidine and putrescine on modulation by ATP, K?, Na?, NH4? and Mg²? and on inhibition by ouabain of posterior gill microsomal Na?,K?-ATPase activity in the blue crab, Callinectes ornatus, acclimated to a dilute medium (21‰ salinity). This is the first kinetic demonstration of competition between spermine and spermidine for the cation sites of a crustacean Na?,K?-ATPase. Polyamine inhibition is enhanced at low cation concentratio ...	2011	21972069
Marine debris impacts to a tidal fringing-marsh in North Carolina.	We evaluated injuries to Spartina alterniflora by debris items common to North Carolina coastal waters as a function of debris type (wire blue crab pots, vehicle tires, and anthropogenic wood) and deployment duration, and monitored S. alterniflora recovery following debris removal. Injuries sustained by S. alterniflora and subsequent recovery, varied considerably between debris types. Differences were likely due to dissimilarities in the structure and composition of debris. Tires caused an immed ...	2011	22051039
oyster reefs as natural breakwaters mitigate shoreline loss and facilitate fisheries.	shorelines at the interface of marine, estuarine and terrestrial biomes are among the most degraded and threatened habitats in the coastal zone because of their sensitivity to sea level rise, storms and increased human utilization. previous efforts to protect shorelines have largely involved constructing bulkheads and seawalls which can detrimentally affect nearshore habitats. recently, efforts have shifted towards "living shoreline" approaches that include biogenic breakwater reefs. our study e ...	2011	21850223
discovery and functional study of a novel crustacean tachykinin neuropeptide.	tachykinin-related peptide (trp) refers to a large and structurally diverse family of neuropeptides found in vertebrate and invertebrate nervous systems. these peptides have various important physiological functions, from regulating stress in mammals to exciting the pyloric (food filtering) rhythm in the stomatogastric nervous system (stns) of decapod crustaceans. here, a novel trp, which we named calstrp (callinectes sapidus trp), ypsgflgmramide (m/z 1026.52), was identified and de novo sequenc ...	2011	22247794
evaluation of parenteral drugs for anesthesia in the blue crab (callinectes sapidus).	the objective of this study was to evaluate the efficacy and safety of several parenteral anesthetics in blue crabs (callinectes sapidus). thirty-one animals were administered one or more of the following drugs by injection into the hemolymph (i.v.) through an arthrodial membrane: etomidate, ketamine, lidocaine, pentobarbital, propofol, tiletamine-zolazepam, xylazine, and ketamine-xylazine. a subset of crabs received intracardiac ketamine. etomidate had no effect. lidocaine effects were ultrasho ...	2011	22946408
identification of the major allergens of charybdis feriatus (red crab) and its cross-reactivity with portunus pelagicus (blue crab).	tropomyosin and arginine kinase have been identified as the major allergens in multiple species of crab. charybdis feriatus is an important commercial crab in this country.	2012	23393908
plastic parasites: extreme dimorphism creates a taxonomic conundrum in the phylum microsporidia.	in this paper, we combine field observations of highly statistically significant co-occurrence with histopathological, ultrastructural and molecular phylogenetic analyses, to provide evidence for extreme morphological plasticity in a microsporidium parasite infecting the musculature of marine crabs. the parasite appears to alternate between lineages that culminate in production of either bizarre needle-like spores in the peripheral sarcoplasm of heart and skeletal muscle fibres (reminiscent of n ...	2012	23262304
cloning of aquaporin-1 of the blue crab, callinectes sapidus: its expression during the larval development in hyposalinity.	ontogenetic variation in salinity adaptation has been noted for the blue crab, callinectes sapidus, which uses the export strategy for larval development: females migrate from the estuaries to the coast to spawn, larvae develop in the ocean, and postlarvae (megalopae) colonize estuarine areas. we hypothesized that c. sapidus larvae may be stenohaline and have limited osmoregulatory capacity which compromises their ability to survive in lower salinity waters. we tested this hypothesis using hatch ...	2012	22943628
advances in our understanding of the global diversity and distribution of hematodinium spp. - significant pathogens of commercially exploited crustaceans.	hematodinium species are parasitic dinoflagellates known to infect a growing number of marine crustacean genera from around the world, many of which support important commercial fisheries. affected hosts undergo dramatic pathological alterations to their organs, tissues and hemolymph. there are no known control measures for this disease. economically important wild fished hosts known to be susceptible to hematodinium spp. include tanner crabs chionoecetes bairdi and snow crabs chionoecetes opili ...	2012	22433998
prevalence, characterization and sources of listeria monocytogenes in blue crab (callinectus sapidus) meat and blue crab processing plants.	seven blue crab processing plants were sampled to determine the prevalence and sources of listeria spp. and listeria monocytogenes for two years (2006-2007). a total of 488 raw crabs, 624 cooked crab meat (crab meat) and 624 environmental samples were tested by standard methods. presumptive listeria spp. were isolated from 19.5% of raw crabs, 10.8% of crab meat, and 69.5% of environmental samples. l. monocytogenes was isolated from 4.5% of raw crabs, 0.2% of crab meat, and 2.1% of environmental ...	2012	22608232
pressure-assisted capillary electrophoresis coupling with matrix-assisted laser desorption/ionization-mass spectrometric imaging for quantitative analysis of complex peptide mixtures.	herein, we report a pressure-assisted capillary electrophoresis-mass spectrometric imaging (pace-msi) platform for peptide analysis. this new platform has addressed the sample diffusion and peak splitting problems that appeared in our previous groove design, and it enables homogeneous deposition of the ce trace for high-throughput maldi imaging. in the coupling of ce to msi, individual peaks (m/z) can be visualized as discrete colored image regions and extracted from the ms imaging data, thus el ...	2012	22891936
poly(glycidyl methacrylate-divinylbenzene) based immobilized ph gradient capillary isoelectric focusing coupling with maldi mass spectrometry for enhanced neuropeptide analysis.	herein, we report an immobilized ph gradient (ipg) capillary isoelectric focusing-matrix-assisted laser desorption/ionization mass spectrometry (cief-maldi ms) platform designed for the separation of complex neuropeptides. this platform features a poly(glycidyl methacrylate-divinylbenzene) (gma-dvb)-based monolithic column for cief separation. different from regular cief, carrier ampholytes are preimmobilized on the monolithic surface instead of being added to the sample. an off-line coupling of ...	2012	22451059
A second copper zinc superoxide dismutase (CuZnSOD) in the blue crab Callinectes sapidus: Cloning and up-regulated expression in the hemocytes after immune challenge.	The full-length cDNA (1362 nucleotides, GenBank JF736621) encoding an extracellular copper zinc superoxide dismutase initially isolated from an EST library of the blue crab Callinectes sapidus was characterized using 3' RACE and named Cas-ecCuZnSOD-2. The open reading frame of Cas-ecCuZnSOD-2 contains 203 deduced amino acids with the conserved active catalytic center for copper and zinc binding and the post-translational modification at two putative N-glycosylation and nine phosphorylation sites ...	2012	22056499
the geography of mercury and pcbs in north carolina's local seafood.	mercury and pcbs are used by non-governmental organizations and federal agencies to inform seafood safety recommendations. pollution dynamics suggest recommendations on the national scale may be too large to be accurate. we tested softshell and hardshell blue crab, white and pink shrimp, oysters, clams, spot, and mullet from fishers in each of the three north carolina fishery districts. we measured mercury using epa method 7473 and pcbs using a commercially available elisa kit. over 97% of sampl ...	2012	22658912
temporal distribution of genetically homogenous 'free-living' hematodinium sp. in a delmarva coastal ecosystem.	significant damage to crustacean fisheries worldwide has been associated with hematodinium sp. it has been postulated that hematodinium sp. requires passage through the water column and/or intermediate hosts to complete its life cycle. thus, an understanding of the prevalence and seasonality of hematodinium sp. within environmentally-derived samples should yield insight into potential modes of disease transmission, and how these relate to infection cycles in hosts.	2012	22828185
assessment of metal concentrations in muscles of the blue crab, callinectes danae s., from the santos estuarine system.	this study determined the concentrations of eleven metals in the blue crab, callinectes danae, from nine sites in the santos estuarine system of sao paulo state, brazil. the results were compared to guidelines established in the united states, europe and brazil for the safety of human consumers. muscles of blue crabs were removed by dissection and concentrations of al, cd, co, cr, cu, fe, hg, mn ni, pb and zn were determined. in general, the concentrations of metals were low, and the crabs were ...	2012	22782356
a preliminary assessment of metal bioaccumulation in the blue crab, callinectes danae s., from the sao vicente channel, sao paulo state, brazil.	the concentrations of metals in tissues of callinectes danae were evaluated, aiming to determine the bioaccumulation process of this species. gills presented the highest mean concentrations for most metals, except for hg (<lod) and zn (13.293 μg g(-1)). according to results of the principal component analysis, the type of tissue was distinguished due to the concentrations of metals. an evaluation of individuals from other sites of the santos estuarine system (where the sao vicente channel is inc ...	2012	22349281
absence of ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in c. crangon and p. serratus.	mitochondria from the embryos of brine shrimp (artemia franciscana) do not undergo ca(2+)-induced permeability transition in the presence of a profound ca(2+) uptake capacity. furthermore, this crustacean is the only organism known to exhibit bongkrekate-insensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of ca(2+)-induced permeability transition are somehow related phenomena. here we report that mitochondria isolated fro ...	2012	22768139
bioinspired algorithm for autonomous sensor-driven guidance in turbulent chemical plumes.	we designed and implemented a control algorithm for sensor-mediated chemical plume tracking in a turbulent flow environment. in our design, we focused on development of a signal processing strategy capable of replicating behavioral responses of actively tracking blue crabs (callinectes sapidus) to chemical stimuli. the control algorithm is evaluated in a hardware platform that allows motion in two directions (i.e. forward-back and left-right). the geometric arrangement of the sensor array is ins ...	2012	22728917
stage-specific changes in calcium concentration in crustacean (callinectes sapidus) y-organs during a natural molting cycle, and their relation to the hemolymphatic ecdysteroid titer.	secretion of ecdysteroid molting hormones by crustacean y-organs is suppressed by molt-inhibiting hormone (mih). the suppressive effect of mih on ecdysteroidogenesis is mediated by one or more cyclic nucleotide second messengers. in addition, existing data indicate that ecdysteroidogenesis is positively regulated (stimulated) by intracellular ca(++). despite the apparent critical role of calcium in regulating ecdysteroidogenesis, the level of ca(++) in y-organ cells has not been previously measu ...	2012	22683690
gene transcripts encoding hypoxia-inducible factor (hif) exhibit tissue- and muscle fiber type-dependent responses to hypoxia and hypercapnic hypoxia in the atlantic blue crab, callinectes sapidus.	hypoxia inducible factor (hif) is a transcription factor that under low environmental oxygen regulates the expression of suites of genes involved in metabolism, angiogenesis, erythropoiesis, immune function, and growth. here, we isolated and sequenced partial cdnas encoding hif-α and arnt/hif-β from the atlantic blue crab, callinectes sapidus, an estuarine species that frequently encounters concurrent hypoxia (low o(2)) and hypercapnia (elevated co(2)). we then examined the effects of acute expo ...	2012	22643338
mass spectrometric elucidation of the neuropeptidome of a crustacean neuroendocrine organ.	the blue crab callinectes sapidus has been used as an experimental model organism for the study of regulation of cardiac activity and other physiological processes. moreover, it is an economically and ecologically important crustacean species. however, there was no previous report on the characterization of its neuropeptidome. to fill in this gap, we employed multiple sample preparation methods including direct tissue profiling, crude tissue extraction and tissue extract fractionation by hplc to ...	2012	22627023
oesophageal chemoreceptors of blue crabs, callinectes sapidus, sense chemical deterrents and can block ingestion of food.	decapod crustaceans such as blue crabs possess a variety of chemoreceptors that control different stages of the feeding process. all these chemoreceptors are putative targets for feeding deterrents that cause animals to avoid or reject otherwise palatable food. as a first step towards characterizing the chemoreceptors that mediate the effect of deterrents, we used a behavioral approach to investigate their precise location. data presented here demonstrate that chemoreceptors located on the anten ...	2012	22539737
interactions between multiple recruitment drivers: post-settlement predation mortality and flow-mediated recruitment.	dispersal is a primary driver in shaping the future distribution of species in both terrestrial and marine systems. physical transport by advection can regulate the distance travelled and rate of propagule supply to a habitat but post-settlement processes such as predation can decouple supply from recruitment. the effect of flow-mediated recruitment and predation on the recruitment success of an intertidal species, the eastern oyster crassostrea virginica was evaluated in two-replicated field ex ...	2012	22493734
the male blue crab, callinectes sapidus, uses both chromatic and achromatic cues during mate choice.	in the blue crab, callinectes sapidus, claw color varies by sex, sexual maturity and individual. males rely in part on color cues to select appropriate mates, and these chromatic cues may be perceived through an opponent interaction between two photoreceptors with maximum wavelength sensitivities at 440 and 508 nm. the range of color discrimination of this dichromatic visual system may be limited, however, and it is unclear whether male blue crabs are capable of discriminating the natural variat ...	2012	22399664
the effect of salinity on experimental infections of a hematodinium sp. in blue crabs, callinectes sapidus.	the parasitic dinoflagellate hematodinium sp. parasitizes blue crabs along the atlantic seaboard of the united states. infections in blue crabs have only been reported from waters where salinity is >11 practical salinity units (psu). blue crabs maintain a hyperosmotic internal concentration at low salinities (0-5 psu), roughly comparable to 24 psu, and should be capable of maintaining an infection in low-salinity waters even if hematodinium spp. cells are intolerant of low salinities. we tested ...	2012	22257093
new functions of arthropod bursicon: inducing deposition and thickening of new cuticle and hemocyte granulation in the blue crab, callinectes sapidus.	arthropod growth requires molt-associated changes in softness and stiffness of the cuticle that protects from desiccation, infection and injury. cuticle hardening in insects depends on the blood-borne hormone, bursicon (burs), although it has never been determined in hemolymph. whilst also having burs, decapod crustaceans reiterate molting many more times during their longer life span and are encased in a calcified exoskeleton, which after molting undergoes similar initial cuticle hardening proc ...	2012	23029467
use of fully biodegradable panels to reduce derelict pot threats to marine fauna.	fishing pots (i.e., traps) are designed to catch fish or crustaceans and are used globally. lost pots are a concern for a variety of fisheries, and there are reports that 10-70% of deployed pots are lost annually. derelict fishing pots can be a source of mortality for target and bycatch species for several years. because continual removal of derelict gear can be impractical over large spatial extents, modifications are needed to disarm gear once it is lost. we tested a fully biodegradable panel ...	2012	22988935
a neighboring plant species creates associational refuge for consumer and host.	examples of plant-animal and plant-plant associational defenses are common across a variety of systems, yet the potential for them to occur in concert has not been explored. in salt marshes in the gulf of mexico, the marsh periwinkle (littoraria irrorata) is an abundant and conspicuous member of the community, climbing up the stems of marsh plants to remain out of the water at high tide. though littoraria are thought to primarily utilize stems of marsh cordgrass spartina alterniflora as a source ...	2012	22834381
biotic structure indirectly affects associated prey in a predator-specific manner via changes in the sensory environment.	indirect effects, which can be either positive or negative, may be important in areas containing biotic structure, because such structure can provide refuge and habitat, produce additional sensory cues that may attract predators, and modify the sensory landscape in which predator-prey interactions occur. to determine the indirect effects of biotic structure on prey populations, we assessed predation on patches of hard clams (mercenaria mercenaria) by large odor-mediated blue crab (callinectes sa ...	2013	22821422
extracellular digestion during hyposaline exposure in the dungeness crab, cancer magister, and the blue crab, callinectes sapidus.	extracellular digestive processes were examined in the dungeness crab, cancer magister and the blue crab, callinectes sapidus, during hyposaline exposure. both species are found in estuaries as adults, but vary in their ability to balance the cardiovascular and respiratory demands of concurrent osmoregulation and digestion. the weak osmoregulator, c. magister, is unable to balance the demands of osmoregulation and digestion. concordant with observed decreases in oxygen consumption and mechanical ...	2013	24035839
cloning of prophenoloxidase from hemocytes of the blue crab, callinectes sapidus and its expression and enzyme activity during the molt cycle.	the arthropods cuticle undergoes dramatic morphological and biochemical changes from being soft to hardness through each molting process. prophenoloxidase (ppo) known as a key enzyme in the arthropod innate immune system involved in the melanization reaction, has been related with the initial shell-hardening process, specifically in the sclerotization of the protein matrix in the new cuticle. since hemocytes have been reported as the main ppo source in arthropods, the transport of hemocyte ppo i ...	2013	23968691
effect of meal size and body size on specific dynamic action and gastric processing in decapod crustaceans.	meal size and animal size are important factors affecting the characteristics of the specific dynamic action (sda) response across a variety of taxa. the effects of these two variables on the sda of decapod crustaceans are based on just a couple of articles, and are not wholly consistent with the responses reported for other aquatic ectotherms. therefore, the effects of meal size and animal size on the characteristics of sda response were investigated in a variety of decapod crustaceans from dif ...	2013	23916818
fecundity, reproductive seasonality and maturation size of callinectes sapidus females (decapoda: portunidae) in the southeast coast of brazil.	c sapidus fisheries has a significant influence on the economy of some countries in north america and has a relative extensive literature in these regions. however, only few papers discuss the ecology of c. sapidus in the south atlantic, despite its economic importance in that region. we studied the fecundity, reproductive seasonality and maturation size of c. sapidus females captured in the southeast coast of brazil from january to december 2002. females were separated, weighted (wt), and cepha ...	2013	23885578
a multi-scale strategy for discovery of novel endogenous neuropeptides in the crustacean nervous system.	the conventional mass spectrometry (ms)-based strategy is often inadequate for the comprehensive characterization of various size neuropeptides without the assistance of genomic information. this study evaluated sequence coverage of different size neuropeptides in two crustacean species, blue crab callinectes sapidus and jonah crab cancer borealis using conventional ms methodologies and revealed limitations to mid- and large-size peptide analysis. herein we attempt to establish a multi-scale str ...	2013	23806756
ecdysone and retinoid-x receptors of the blue crab, callinectes sapidus: cloning and their expression patterns in eyestalks and y-organs during the molt cycle.	crustacean molting is known to be regulated largely by ecdysteroids and crustacean hyperglycemic hormone (chh) neuropeptide family including molt-inhibiting hormone (mih) and chh. the surge of 20-oh ecdysone and/or ponasterone a initiates the molting process through binding to its conserved heterodimeric nuclear receptor: ecdysone receptor (ecr) and ultraspiracle (usp)/retinoid-x receptor (rxr). to better understand the role of ecdysteroids in the molt regulation, the full-length cdnas of the bl ...	2013	23764560
locomotory fatigue during moderate and severe hypoxia and hypercapnia in the atlantic blue crab, callinectes sapidus.	the atlantic blue crab, callinectes sapidus (rathbun), is a highly mobile crustacean that must locomote to find food, evade predators, find mates, and avoid adverse conditions such as hypoxia. in this study we tested the effects of two levels of hypoxia (10.4 kpa, 50% air saturation = moderate hypoxia; 4 kpa, 20% air saturation = severe hypoxia) and hypercapnic hypoxia (50% air saturation o(2) with pco(2) = 2 kpa) on fatigue during sustained continuous exercise. fatigue was induced by an exercis ...	2013	23677972
locomotory fatigue and size in the atlantic blue crab, callinectes sapidus.		2013	23677971
molecular cloning of a plasma membrane ca²⁺ atpase (pmca) from y-organs of the blue crab (callinectes sapidus), and determination of spatial and temporal patterns of pmca gene expression.	existing data indicate that a stage-specific increase in intracellular free ca(2+) stimulates ecdysteroid production by crustacean molting glands (y-organs). the concentration of ca(2+) in cytosol is controlled mainly by proteins intrinsic to the plasma membrane and to the membranes of organelles. several families of proteins are involved, including ca(2+) channels, ca(2+) pumps (atpases), and ca(2+) exchangers. the family of ca(2+) pumps includes plasma membrane calcium atpases (pmcas). as a st ...	2013	23545309
mass spectrometric evaluation of neuropeptidomic profiles upon heat stabilization treatment of neuroendocrine tissues in crustaceans.	tissue heat stabilization is a vital component in successful mammalian neuropeptidomic studies. heat stabilization using focused microwave irradiation, conventional microwave irradiation, boiling, and treatment with the denator stabilizor t1 have all proven effective in arresting post-mortem protein degradation. although research has reported the presence of protein fragments in crustacean hemolymph when protease inhibitors were not added to the sample, the degree to which post-mortem protease a ...	2013	23227893
mapping of neuropeptides in the crustacean stomatogastric nervous system by imaging mass spectrometry.	considerable effort has been devoted to characterizing the crustacean stomatogastric nervous system (stns) with great emphasis on comprehensive analysis and mapping distribution of its diverse neuropeptide complement. previously, immunohistochemistry (ihc) has been applied to this endeavor, yet with identification accuracy and throughput compromised. therefore, molecular imaging methods are pursued to unequivocally determine the identity and location of the neuropeptides at a high spatial resolu ...	2013	23192703
efficacy of alfaxalone for intravascular anesthesia and euthanasia in blue crabs (callinectes sapidus).	the objective of this study was to characterize the behavioral effects and changes in heart rate of four doses of alfaxalone delivered by intravascular injection to blue crabs (callinectes sapidus). thirty (male, n = 27; female, n = 3) blue crabs were randomly assigned to one of four treatment groups of alfaxalone: eight animals were assigned to each of the 5-, 10-, and 15-mg/kg treatment groups, and the remaining six animals were assigned to the 100-mg/kg group. times for anesthetic induction a ...	2013	24063098
liquid chromatography-matrix-assisted laser desorption/ionization mass spectrometric imaging with sprayed matrix for improved sensitivity, reproducibility and quantitation.	matrix-assisted laser desorption/ionization (maldi) mass spectrometric imaging (msi) has been employed as a detection method for both capillary electrophoresis (ce)-maldi and liquid chromatography (lc)-maldi analyses. based on our previous studies, here we report a new interface to couple lc with msi by employing an automated matrix sprayer. the lc trace is directly collected on a ground stainless steel maldi plate and dried. the matrix is sprayed on the maldi plate using a programmable matrix s ...	2013	24003441
semi-automated liquid chromatography-mass spectrometric imaging platform for enhanced detection and improved data analysis of complex peptides.	a semi-automated analytical platform featuring the coupling of monolithic reversed-phase liquid chromatography (rplc) to matrix-assisted laser desorption/ionization mass spectrometric imaging (maldi msi) has been developed and evaluated. this is the first time that lc separation is readily coupled to ms imaging detection for the analysis of complex peptide mixtures both qualitatively and quantitatively. methacrylate-based monolithic column with c12 functional groups is fabricated for fast rplc s ...	2013	23623366
conservation in the first internal transcribed spacer (its1) region of hematodinium perezi (genotype iii) from callinectes sapidus .	hematodinium spp. infections have been reported from blue crabs callinectes sapidus in high-salinity waters of the usa from new jersey to texas. recently, h. perezi (genotype iii) has been proposed as the parasite species and genotype infecting blue crabs from virginia; however, it is unknown whether this same genotype is present in blue crabs from other locations. to address this question, we collected 317 blue crabs from massachusetts, virginia, georgia, florida, louisiana, and texas to test f ...	2013	23482386
experimental infections of orchitophrya stellarum (scuticociliata) in american blue crabs (callinectes sapidus) and fiddler crabs (uca minax).	outbreaks of an unidentified ciliate have occurred on several occasions in blue crabs from chesapeake bay held during winter months in flow-through systems. the parasite was initially thought to be mesanophrys chesapeakensis, but molecular analysis identified it as orchitophyra stellarum, a facultative parasite of sea stars (asteroidea). we investigated the host-parasite association of o. stellarum in the blue crab host. crabs were inoculated with the ciliate, or they were held in bath exposures ...	2013	24018170
discovery of an opportunistic starfish pathogen, orchitophrya stellarum, in captive blue crabs, callinectes sapidus.	histophagous scuticociliate infections were discovered in blue crabs, callinectes sapidus, held in research facilities at the virginia institute of marine science. ciliates were observed infecting every tissue examined including the gills, heart, muscle, hepatopancreas, and epidermis. hemolymph smears and histological tissue sections indicated a morphological similarity to mesanophrys chesapeakensis, the only recorded histophagous ciliate infecting blue crabs. however, subsequent analysis of the ...	2013	23954308
a comparison of two methods for colorimetric in situ hybridization using paraffin-embedded tissue sections and digoxigenin-labeled hybridization probes.	two methods for colorimetric in situ dna probe hybridization (cish) assays on paraffin-embedded tissue sections were compared. the heated method used heat (90-100°c) to denature dna in the sample prior to probe hybridization, while the unheated method used a standard hybridization temperature of 42°c. both procedures were tested on tissue samples that harbored the mollusk protozoan pathogens perkinsus marinus, p. chesapeaki, or haplosporidium nelsoni, the protozoan and bacterial fish pathogens m ...	2013	23697605
variation in spatial and temporal incidence of the crustacean pathogen hematodinium perezi in environmental samples from atlantic coastal bays.	hematodinium perezi, a parasitic dinoflagellate, infects and kills blue crabs, callinectes sapidus, along the atlantic and gulf coasts of the united states. the parasite proliferates within host hemolymph and tissues, and also produces free-swimming biflagellated dinospores that emerge from infected crabs. infections in c. sapidus recur annually, and it is not known if biotic or environmental reservoirs contribute to reinfection and outbreaks. to address this data gap, a quantitative pcr assay b ...	2013	23641869
molecular characterization of a cytosolic manganese superoxide dismutase from the chinese mitten crab, eriocheir sinensis.	a cytosolic manganese superoxide dismutase gene (es-cmnsod) was cloned from the chinese mitten crab eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cdna ends. the open reading frame of es-cmnsod is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. the calculated molecular mass of the translated protein of es-cmnsod is 31.43 kda, with an estimated isoelectric point of 6.30. the deduced amino acid sequence of es ...	2014	25501153
trehalose metabolism in the blue crab callinectes sapidus: isolation of multiple structural cdna isoforms of trehalose-6-phosphate synthase and their expression in muscles.	adult blue crab callinectes sapidus exhibit behavioral and ecological dimorphisms: females migrating from the low salinity water to the high salinity area vs. males remaining in the same areas. the flesh basal muscle of the swimming paddle shows a dimorphic color pattern in that levator (lev) and depressor (dep) of females tend to be much darker than those of males, while both genders have the same light colored remoter (rem) and promoter (pro). the full-length cdna sequence of four structural i ...	2014	24334121
d1-like dopamine receptors downregulate na+-k+-atpase activity and increase camp production in the posterior gills of the blue crab callinectes sapidus.	dopamine-mediated regulation of na(+)-k(+)-atpase activity in the posterior gills of some crustaceans has been reported to be involved in osmoregulation. the dopamine receptors of invertebrates are classified into three groups based on their structure and pharmacology: d1- and d2-like receptors and a distinct invertebrate receptor subtype (indr). we tested the hypothesis that a d1-like receptor is expressed in the blue crab callinectes sapidus and regulates na(+)-k(+)-atpase activity. rt-pcr, us ...	2014	25080496
prevalence of vibrio parahaemolyticus, and vibrio vulnificus in blue crabs (callinectes sapidus), seawater and sediments of the maryland coastal bays.	to determine the prevalence of total and pathogenic vibrio parahaemolyticus (vp) and v. vulnificus (vv) in blue crabs, water and sediment from the maryland coastal bays (mcbs), usa.	2014	25066367
spontaneous alternation and locomotor activity in three species of marine crabs: green crab (carcinus maenas), blue crab (callinectes sapidus), and fiddler crab (uca pugnax).	spontaneous alternation refers to the tendency of organisms to explore places that they have least recently visited. our previous work showed that alternation performance of carcinus maenas (invasive european green crab) was significantly higher than callinectes sapidus (native blue crab), and chance level performance (ramey, p. a., teichman, e., oleksiak, j., & balcı, f. [2009]. spontaneous alternation in marine crabs: invasive versus native species. behavioural processes, 82, 51-55.). in the c ...	2014	24060243
an insulin-like growth factor found in hepatopancreas implicates carbohydrate metabolism of the blue crab callinectes sapidus.	hyperglycemia that is caused by the release of crustacean hyperglycemic hormone (chh) from the sinus gland to hemolymph is one of the hallmark physiological phenomena, occurring in decapod crustaceans experiencing stressful conditions. however, the mechanism(s) by which such elevated glucose levels return to resting levels is still unknown. interestingly, noted is a difference in the clearance rate of hemolymph glucose between adult females and adult males of the blue crab, callinectes sapidus: ...	2014	24503150
metal, metallothionein and glutathione levels in blue crab (callinectes sp.) specimens from southeastern brazil.	metal concentrations (cu, pb, zn and cd) were determined in muscle, gills, soft tissues and eggs in male, non-ovigerous and ovigerous female callinectes sp. specimens from a reference site in southeastern brazil. metallothionein (mt) and reduced glutathione (gsh) levels were also determined. results demonstrate that sex has a significant influence on metal, mt and gsh concentrations. significant maternal transfer of pb and zn from ovigerous females to eggs was verified, while female crabs, both ...	2014	24905697
distribution of heavy metals in muscles and internal organs of korean cephalopods and crustaceans: risk assessment for human health.	samples of seven species of cephalopods and crustaceans were collected from major fish markets on the korean coast and analyzed for mercury (hg) using a direct hg analyzer and for the metals cadmium (cd), lead (pb), chromium, silver, nickel, copper, and zinc using inductively coupled plasma mass spectrometry. the distributions of heavy metals in muscles, internal organs, and whole tissues were determined, and a risk assessment was conducted to provide information concerning consumer safety. the ...	2014	25474068
detecting selection in the blue crab, callinectes sapidus, using dna sequence data from multiple nuclear protein-coding genes.	the identification of genes involved in the adaptive evolution of non-model organisms with uncharacterized genomes constitutes a major challenge. this study employed a rigorous and targeted candidate gene approach to test for positive selection on protein-coding genes of the blue crab, callinectes sapidus. four genes with putative roles in physiological adaptation to environmental stress were chosen as candidates. a fifth gene not expected to play a role in environmental adaptation was used as a ...	2014	24896825
mercury, lead, and cadmium in blue crabs, callinectes sapidus, from the atlantic coast of florida, usa: a multipredator approach.	blue crabs, callinectes sapidus, from the atlantic coast of florida were analyzed for total mercury, methylmercury, lead, and cadmium. paired samples of two tissue types were analyzed for each crab, (1) muscle tissue (cheliped and body muscles) and (2) whole-body tissue (all organs, muscle tissue and connective tissue), for evaluation of the concentration of metals available to human consumers as well as estuarine predators. there were clear patterns of tissue-specific partitioning for each meta ...	2014	24507459
a new pcr-based method shows that blue crabs (callinectes sapidus (rathbun)) consume winter flounder (pseudopleuronectes americanus (walbaum)).	winter flounder (pseudopleuronectes americanus) once supported robust commercial and recreational fisheries in the new york (usa) region, but since the 1990s populations have been in decline. available data show that settlement of young-of-the-year winter flounder has not declined as sharply as adult abundance, suggesting that juveniles are experiencing higher mortality following settlement. the recent increase of blue crab (callinectes sapidus) abundance in the new york region raises the possib ...	2014	24454797
the smell of moulting: n-acetylglucosamino-1,5-lactone is a premoult biomarker and candidate component of the courtship pheromone in the urine of the blue crab, callinectes sapidus.	female blue crabs (callinectes sapidus) in their pubertal moult stage release unidentified sex pheromone molecules in their urine, causing males to respond with courtship behaviours including a display called courtship stationary paddling and a form of precopulatory guarding called cradle carry. we hypothesized that pheromones are mixtures of molecules and are more concentrated in urine of pubertal premoult females compared with other moulting stages and thus that these molecules are biomarkers ...	2014	24363413
ghost fishing activity in derelict blue crab traps in louisiana.	derelict crab traps impact the coastal ecosystem through continued catch of target species and species of conservation, economic, or recreational importance. during volunteer-supported crab trap cleanups in 2012 and 2013, we quantified ghost fishing activity in derelict crab traps in coastal louisiana through a citizen scientist program. volunteers removed 3607 derelict traps during these events, and over 65% of traps analyzed by citizen scientists were actively ghost fishing. additionally, volu ...	2014	24360333
carbonic anhydrase induction in euryhaline crustaceans is rate-limited at the post-transcriptional level.	the transfer of euryhaline crustaceans from full-strength seawater to low salinity results in both a rapid up-regulation of carbonic anhydrase (ca; ec 4.2.1.1) mrna and a slow induction of ca activity. there is a delay of several days between the two processes, which is attributed to the time required to synthesize new enzyme. these delays may also be due to limitations in the cellular uptake of zn, which is a required post-translational active site modification to ca. to investigate these proce ...	2014	24333600
a novel hormone is required for the development of reproductive phenotypes in adult female crabs.	the crustacean male-specific androgenic hormone is widely accepted as a key factor in sexual differentiation and in the development of secondary sex characteristics. however, the mechanism by which the plethora of different reproductive strategies are controlled and executed in crustaceans is not known. we discovered in the blue crab, callinectes sapidus, a hitherto unknown neurohormone, named crustacean female sex hormone (cfsh), in distinct neurosecretory cells in the eyestalk ganglia. cfsh is ...	2014	24280057
functional characterization of neuroendocrine regulation of branchial carbonic anhydrase induction in the euryhaline crab callinectes sapidus.	carbonic anhydrase (ca) plays an essential role as a provider of counterions for na(+)/h(+) and cl(-)/hco3 (-) exchange in branchial ionic uptake processes in euryhaline crustaceans. ca activity and gene expression are low in crabs acclimated to full-strength seawater, with transfer to low salinity resulting in large-scale inductions of mrna and subsequent enzyme activity in the posterior ion-regulating gills (e.g., g7). in the green crab carcinus maenas, ca has been shown to be under inhibitory ...	2014	25572216
trophodynamic behavior of hydrophobic organic contaminants in the aquatic food web of a tidal river.	the bioaccumulation and biomagnification of sediment-bound hydrophobic organic contaminants (hocs) are of major concern for environmental and human health. in dynamic estuaries, hocs can be taken up from sediments, porewater, or the overlying water column concentrations directly or via the diet. the transfer of hocs including polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and polychlorinated dibenzo-p-dioxins/furans (pcdd/fs) to resident/migratory biota was investigat ...	2014	25272053
toxicity of the dispersant corexit 9500 to early life stages of blue crab, callinectes sapidus.	the deepwater horizon well released 4.4 million barrels of light crude oil offshore of louisiana into one of the world's largest and most productive blue crab (callinectes sapidus) fisheries. the objectives of this paper were to determine the toxicity of the dispersant corexit(®) 9500a used in the 2010 oil spill on juvenile and larval blue crabs, and the long-term effects of sublethal acute exposure. only the highest treatment levels of dispersant significantly increased mortality in larval and ...	2014	25173366