Publications

the adsorption of bacteria to immobilized lectins.	the agglutination of a selection of bacteria by some lectins was examined. the lectin from codium fragile agglutinated seven strains of salmonella typhimurium. the lectin from helix pomatia agglutinated eight of 12 strains of listeria monocytogenes and a further two strains gave a weak agglutination reaction. helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of l. monocytogenes from suspension with subsequent elution by the competing ligand n-acetyl galactosamine. a ...	1991	1955420
expression of blood group-related antigens and helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma.	in an attempt to differentiate malignant pleural mesothelioma from pulmonary adenocarcinoma by histochemical and immunohistochemical means, the glycoconjugate profiles of five reactive mesothelial lesions, 29 mesotheliomas (20 epithelial, three biphasic, and six fibrous types), and 38 well-differentiated pulmonary adenocarcinomas (34 papillary, two tubular, and two bronchioloalveolar types) were tested with abh blood group-related antigens (bgr-ag) antibody and helix pomatia agglutinin (hpa) whi ...	1991	2001875
binding of platelet agglutinating protein p37 from the plasma of a patient with thrombotic thrombocytopenic purpura to human platelets.	we have previously reported the purification of a 37 kda platelet agglutinating protein (pap p37) from the plasma of a patient with thrombotic thrombocytopenic purpura (ttp) that was shown to be present in a subset of ttp patients. to gain further insight into the interaction between pap p37 and platelets, we have studied the properties of pap p37 binding to platelets. washed human platelets from two normal donors and two ttp patients after recovery were used for the experiments. the pap p37 bin ...	1991	2024244
lectin histochemistry of developing submandibular glands of fetal syrian golden hamsters.	lectin binding was studied in the developing submandibular glands of fetal syrian golden hamsters (mesocricetus auratus) from gestational day 12 to 16 (the day of birth). the fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin a (con a), wheat germ agglutinin (wga), dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), maclura pomifera agglutinin (mpa). griffonia simplicifolia agglutinin i-b4 (gsa i-b4), p ...	1991	2035849
experimental epilepsy in vitro: neuromodulating activity of anti-brain autoantibodies from rats exposed to electroconvulsive shock.	neuromodulating activity of anti-brain autoantibodies obtained from electroshocked (ecs) rats was tested on the neurons of isolated suboesophageal ganglion of the snail helix pomatia. in 16 out of 18 spontaneously active (pacemaker) neurons, ecs igg containing anti-brain autoantibodies induced short-lasting epileptiform discharges and membrane depolarization. membrane input resistance and time constant decreased, while membrane capacitance increased after addition of ecs igg. amplitude of evoked ...	1991	1723059
cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4.	we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ...	1991	1701188
phosphofructokinase from mollusc muscle is activated by phosphorylation.	phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, helix pomatia (gastropoda), and mussels, mytilus edulis (bivalvia). under denaturing conditions, both enzymes had a molecular mass of 82 kda. in the presence of atp-mg2+, the enzymes were rapidly phosphorylated in vitro by the catalytic subunit of cyclic amp (camp)-dependent protein kinase purified from snail muscle and also by the c subunit of protein kinase from bovine heart. the extent of phosphoryla ...	1991	1654810
the prognostic value of tumor-associated carbohydrate structures correlated with gene amplifications in human breast carcinomas.	the effects of cell surface sugar chains combined with certain gene amplifications of breast cancers on the prognosis of patients were studied and the relationships between the sugar chains of cancer cells and amplifications of the proto-oncogenes c-myc, int-2 and c-erb b-2, evaluated. one hundred and fifty three human breast carcinoma tissues were investigated by an immunohistochemical technique using the avidinbiotin-peroxidase method with 1 lectin (hpa; helix pomatia) and 4 monoclonal antibod ...	1991	1687600
kaliotoxin, a novel peptidyl inhibitor of neuronal bk-type ca(2+)-activated k+ channels characterized from androctonus mauretanicus mauretanicus venom.	a peptidyl inhibitor of the high conductance ca(2+)-activated k+ channels (kca) has been purified to homogeneity from the venom of the scorpion androctonus mauretanicus mauretanicus. the peptide has been named kaliotoxin (ktx). it is a single 4-kda polypeptide chain. its complete amino acid sequence has been determined. ktx displays sequence homology with other scorpion-derived inhibitors of ca(2+)-activated or voltage-gated k+ channels: 44% homology with charybdotoxin (ctx), 52% with noxiustoxi ...	1992	1730708
modulation of voltage-activated ion currents on identified neurons of helix pomatia l. by interleukin-1.	1. the effect of interleukin-1 (il-1) was studied on voltage-activated ion currents of the identified central neurons of helix pomatia l. using a two-microelectrode voltage clamp. the voltage-activated inward current (ica) was decreased, whereas the outward current (i(net) k) was increased by il-1. 2. il-1 affects both the transient and the delayed rectifying potassium currents. the il-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. the threshold concent ...	1992	1281756
comparative study of prostatic carcinoma bone metastasis among japanese in japan and japanese americans and whites in hawaii.	metastatic prostate carcinomas in autopsy cases from three populations 49 cases of indigenous japanese, 29 cases of japanese americans and 14 from whites in hawaii) were compared in terms of their clinicopathological, immunohistochemical (tenascin and ras p21) and lectin binding (helix pomatia antigen, hpa) properties. only the clinicopathological features were analyzed in the cases of whites in hawaii. the results indicate that poorly differentiated carcinoma is less common, whereas distant met ...	1992	1283503
lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (shr).	some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater blood vessels of control (wky) and hypertensive rats (shr). the lectins tested recognized the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-l-fucosyl (ulex europaeus agglutinin, uea-1), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, wga), n-glycolyl-neuraminic acid (limax flavus agglutinin, lfa), and n-acetyl-d-galactosaminyl (helix ...	1992	1297933
early increase in lymphocyte cyclic nucleotide phosphodiesterase activity upon mitogenic activation of human peripheral blood mononuclear cells.	cyclic nucleotide phosphodiesterase activity of human peripheral blood mononuclear cells was significantly increased following a short (30 min) incubation with the mitogenic lectin concanavalin a. con a stimulated phosphodiesterase activity to the same extent whatever the subcellular compartment (homogenate, cytosol or pellet). further separation of the con a-activated mononuclear cells into lymphocyte-enriched and monocyte-enriched populations showed that the con a-induced increase of phosphodi ...	1992	1309823
duck lymphocytes--v. transformation responses to phorbol ester and calcium ionophore.	1. lymphocytes purified from duck blood and spleen were cultured in the presence of phorbol 12-myristate 13-acetate (pma) and the calcium ionophore a23187. stimulation was assessed by the incorporation of [3h]thymidine after 3 days' culture. 2. pma stimulated over a wide range of concentrations, with maximum stimulation at final concentrations of 5 x 10(-7)-5 x 10(-8) m/litre. a23187 was effective in the range 5 x 10(-6)-5 x 10(-7) m/litre and also, in some experiments using spleen lymphocytes, ...	1992	1354147
quantitative changes of ricinus communis agglutinin i and helix pomatia lectin binding sites in the acrosome of rat spermatozoa during epididymal transit.	during passage through the epididymis, spermatozoa undergo a number of changes which result in their acquisition of fertility and motility. some of the changes that occur include loss of the cytoplasmic droplet and changes in sperm morphology, metabolism and properties of the nucleus and plasma membrane. changes have also been reported in the acrosomic system of mammalian spermatozoa during their transit through the epididymis. in the present study, the quantitative changes of the glycoconjugate ...	1992	1385371
mannose-induced dysmorphogenesis of metanephric kidney. role of proteoglycans and adenosine triphosphate.	because various fetal anomalies are seen in diabetic offspring, we examined the effects of sugars on proteoglycans (pgs): extracellular matrix (ecm) macromolecules modulating morphogenesis. 13-d-old mouse metanephric kidney explants were exposed to mannose for 7 d and labeled with [35s]sulfate, [35s]-methionine, or [3h]thymidine. mannose exposure caused reduction in kidney size and disorganization of ureteric bud branches with inhibition of glomerulogenesis. tissue autoradiographic and immunoflu ...	1992	1401058
expression of glycoconjugates in the human fetal cochlea.	the distribution of glycoconjugates in the human fetal cochlea was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). the tectorial membrane (tm) in the 11- and 15-week-old human fetuses was labelled with wga, apa, rca120 and cona, but not with uea-i and hpa. in the 19-week-old fetuses, the reaction of the tm ...	1992	1442009
comparative lectin histochemistry on taste buds in foliate, circumvallate and fungiform papillae of the rabbit tongue.	taste buds (tb) in the foliate, circumvallate and fungiform papillae of the rabbit tongue were examined with lectin histochemistry by means of light (lm) and electron (em) microscopy. biotin- and gold-labeled lectins were used for the detection of carbohydrate residues in tb cells and subcutaneous salivary glands. at the lm level, the lectins of soybean (sba) and peanut (pna) react with material of the foliate and circumvallate taste pores only after pretreatment of the section with neuraminidas ...	1992	1452451
lectin binding to secretory structures, the cuticle and the surface coat of toxocara canis infective larvae.	toxocara canis infective larvae are known to produce abundant glycosylated molecules which may be found associated with the surface or secreted into their environment. using a range of fluorescein-conjugated and gold-conjugated lectins, the localization of particular carbohydrates was defined on the surface of live parasites, and internally at the ultrastructural level. surface exposure of n-acetyl galactosamine and n-acetyl glucosamine was deduced by binding of fitc-conjugated helix pomatia (hp ...	1992	1454426
impact of o-glycosylation on the function of human intestinal lactase-phlorizin hydrolase. characterization of glycoforms varying in enzyme activity and localization of o-glycoside addition.	lactase-phlorizin hydrolase (lph) is an integral intestinal brush border membrane glycoprotein responsible for the hydrolysis of lactose, the primary carbohydrate in mammalian milk. to assess the role of n- and o-glycosylation on the function of lph, lectin-binding experiments combined with enzymatic and chemical deglycosylation of purified lph molecules were performed. these investigations provided evidence for the existence of two forms of brush border lph, an n-glycosylated molecule (lphn) an ...	1992	1460044
[characterization of streptococcus uberis with special consideration of supposed pathogenicity factors].	streptococcus uberis, as one of the principal causes of bovine streptococcal mastitis, has been characterized serologically and biochemically. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. the biochemical properties of s. uberis, determined with the strep-zym identification system, differed clearly from those of s. agalactiae and s. dysgalactiae. some cultures of s. uberis produced the enzymes hyaluronidase and neuraminidase. in addition s. uberis pa ...	1992	1497584
separation and identification of the 4-hydroxyantipyrine sulphoconjugate.	in a previous study we observed, during separation of total antipyrine metabolites by high-performance liquid chromatography and after enzymatic hydrolysis, an unidentified peak corresponding to an ionic compound with pyrazolinone features. in the present study, this compound was identified as the 4-hydroxyantipyrine sulphoconjugate, and its structure was definitively confirmed by gas chromatographic-mass spectrometric analysis and by the use of pure synthetic substance. we also demonstrated the ...	1992	1500444
the use of immobilized lectins in the separation of staphylococcus aureus, escherichia coli, listeria and salmonella spp. from pure cultures and foods.	lectins from helix pomatia, canavalia ensiformis, agaricus bisporus and triticum vulgaris agglutinated cultures of staphylococcus aureus, escherichia coli, listeria and salmonella spp. this agglutination was specific as it was inhibited (except with a. bisporus lectin) by the competing sugar substrates. the ability of three of these lectins, immobilized on a variety of supports, to separate these micro-organisms from pure cultures was investigated. immobilization of the lectins on magnetic micro ...	1992	1512177
[characteristics of obtaining protoplasts from 2 strains of tolypocladium inflatum subsp. blastosporum].	the optimal conditions for preparing protoplasts with high yields by using the cells of two (low and high potent) isogenic cyclosporine-producing tolypocladium strains were developed. a specific medium containing 0.5 per cent yeast autolysate (by dry weight) and 3 per cent glucose was used. when grown on this medium the cells of the highly potent strain 847 acquired a yeast-like shape. high yields of protoplasts prepared from the low potent strain 43 mycelium were obtained via prior incubation w ...	1992	1514857
histochemical localization of glycoconjugates in the developing endolymphatic sac and vestibular end organs of the mouse.	the distribution of glycoconjugates in the endolymphatic sac (es) and vestibular end organs from the 12th gestational day (gd) in the developing mouse to the 6th day after birth was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 13th and 15th gd sections, the luminal contents in the fetal es were st ...	1992	1518658
agglutination of bordetella species by lectins.	phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ...	1992	1555759
resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by cell surface lectin cytochemistry.	an intratracheal instillation of human neutrophil elastase (hne) causes accumulation of an excess number of secretory granules in the epithelial secretory cells lining the hamster bronchus. this chronic lesion, which we refer to as secretory cell metaplasia (scm), is not seen in the trachea or bronchioles. because luminal cell surface lectin binding is much higher in the trachea than in the bronchus, we concluded that tracheal resistance may be due to a protective glycoconjugate coat. in the pre ...	1992	1572319
carbohydrates in the cell surface of hair cells from the guinea pig cochlea.	the presence of cell surface carbohydrates was investigated in intact, non-fixed outer hair cells (ohcs) of guinea pigs using fluorescein isothiocyanate (fitc) and rhodamine (tritc) lectins. by means of wheat germ agglutinin (wga) n-acetyl-d-glucosamine was shown in the entire ohc membrane, including the stereocilia. binding of wga in ohcs to neuraminic acid was excluded by preincubation with neuraminidase. moreover, fitc-limulus polyphemus, a specific lectin for neuraminic acid, showed no fluor ...	1992	1581047
intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh.	to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ...	1992	1592875
subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization.	using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rer) in goblet cells of the rat colon. in this cell type, special rer regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. the fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, helix pomatia, griffonia simplicifolia i-a4 and ...	1992	1607641
the hamster transferrin receptor contains ser/thr-linked oligosaccharides: use of a lectin-resistant cho cell line to identify glycoproteins containing these linkages.	we recently reported that the human transferrin receptor (tfr) contains o-linked galnac residues [1]. to investigate whether this modification is shared by transferrin receptors in other mammals, we investigated the glycosylation of tfr in hamster cells. to facilitate our analysis the lectin-resistant chinese hamster ovary (cho) cell line lec8 was used. these cells are unable to galactosylate glycoproteins, resulting in truncation of the ser/thr-linked oligosaccharides to a single residue of ter ...	1992	1640050
developmental changes in the distribution of cecal lectin-binding sites of balb-c mice.	the existence of lectin-binding sites was investigated in the cecum of balb-c mice at seven developmental stages ranging from 18 days post conception (p.c.) to 8 weeks after birth. nine horseradish-peroxidase-conjugated lectins (concanavalin a, triticum vulgaris, dolichus biflorus, helix pomatia, arachis hypogaea, glycine maximus, lotus tetragonolobus, ulex europaeus, limulus polyphemus) were applied to 5- to 7-microns thin paraffin sections of bouin-fixed tissue. after dab staining the sections ...	1992	10457768
ultrastructural, biochemical and electrophysiological changes induced by 5,6-dihydroxytryptamine in the cns of the snail helix pomatia l.	the serotonin neurotoxin, 5,6-dihydroxytryptamine (5,6-dht), was injected into the body cavity of snails. changes induced in the central nervous system (cns) by the neurotoxin were studied by morphological, electrophysiological and biochemical techniques for up to 90 days following injection. the neurotoxin induced a variety of ultrastructural alterations during the early phase (1st to 6th days) following treatment. on day 6 after treatment, membranous structures first appeared in the synaptic-l ...	1992	1324760
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. lectin binding patterns of schwannoma and neurofibroma.	lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. changes of glycoconjugates were observed in association with a neoplastic transformation of schwann cells; arachis hypogaea (pna) staining after neuraminidase treatment seen in normal schwann cells was reduced in schwannoma of antoni type a, ...	1993	8310811
intracisternal crystals in pancreatic acinar cells: failure in the distinct aggregation of secretory proteins.	mechanisms leading to the formation of crystalline inclusions in the cisternal space of the rough endoplasmic reticulum are poorly understood. this phenomenon was investigated in pancreatic acinar cells using two different experimental models: 1) intraperitoneal injection of dl-p-chlorophenylalanine methyl ester, and 2) culture of isolated acinar cells within the matrigel basement membrane in the presence of 2% dimethyl sulfoxide. features and composition of induced crystals were analyzed by pro ...	1993	7523126
[long term survival of first stage larvae of elaphostrongylus cervi (nematoda, metastrongyloidea) in a frozen state].	faeces of red deer from białowieza forest containing first stage larvae of elaphostrongylus cervi have been stored during five years in -20 degrees c. after this time 52% of the larvae survived. these larvae were fully alive and in experimentally infected snails helix pomatia developed to the infectious stage.	1993	8237006
differentiation of pancreatic acinar cells into duct-like cells in vitro.	previous studies have shown that a combination of both the extracellular matrix and secretagogues plays critical roles in the maintenance of well-differentiated pancreatic acinar cells in culture. in the present study, we have shown that, upon proper stimulation, acinar cells change their growth pattern and morphologic appearance to a duct like phenotype.	1993	8246444
histochemistry with helix pomatia agglutinin in human germ cell tumors: detection of nongerminomatous components and correlation between hpa reactivity and radiosensitivity in germinomas.	binding sites of helix pomatia agglutinin (hpa) were examined in 32 patients with intracranial human germ cell tumors. hpa reactivity was found in vascular endothelial cells and erythrocytes of patients with blood type a or ab. hpa-positive neoplastic cells were seen in one yolk sac carcinoma in a patient with blood group a, and in embryonal carcinomas and teratomas irrespective of blood group type. although in 10 out of 18 germinomas neoplastic cells were totally negative for hpa, another 8 ger ...	1993	8252515
developmental changes in neutral glycosphingolipids of mouse placenta.	the mammalian placenta is a unique organ for the study of developmental changes. placentas of laboratory animals such as the mouse allow for the determination of the exact stage of pregnancy, which cannot be achieved with human placenta. in this study, neutral glycosphingolipids were isolated from mouse (inbred strain c57bl/6) placentas, from day 10 to day 18 of gestation, and were separated by high performance thin layer chromatography. densitometric measurements after orcinol staining showed, ...	1993	8257854
affinity methods with lectins: a tool to identify canine alkaline phosphatase isoenzymes.	affinity methods were used to characterize selective interactions of alkaline phosphatase (alp) isoenzymes from different dog tissues with lectins. specific lectins were used to identify liver, intestinal and steroid-induced alp isoenzymes in serum from dogs with cushing syndrome or steroid-treated dogs. for the first approach, 12 lectins were assayed by affinity dots. selective interactions were found among wheat germ agglutinin (wga), jacalin, con a (concanavalin a) and helix pomatia agglutini ...	1993	8258637
histochemical analysis of rat testicular glycoconjugates. 3. non-reducing terminal residues in seminiferous tubules.	lectins of helix pomatia (hpa), glycine max (sba), vicia villosa (vva), dolichos biflorus (dba), ulex europaeus (uea-1), tetragonolobus purpureus (lta), griffonia simplicifolia (bsa-1b4), maclura pomifera (mpa), sambucus nigra (sna) and maackia amurensis (maa) were used to explore the distribution of saccharides characteristic of non-reducing termini of o- and n-linked glycoprotein glycans in the seminiferous tubules of rat testis. sialyl residues (both alpha 2,3- and alpha 2,6-linked, as shown ...	1993	8282567
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man.	the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ...	1993	8310810
the use of lectins identified with specific antibodies in lectin histochemistry of nzb/w f1 mouse kidney.	the affinity of helix pomatia, peanut, pisum sativum, soy bean, and wheat germ agglutinins to various nephron parts of nzb/w f1 mice was different and is assumed to be age dependent. the affinity of pisum sativum agglutinin to basal membranes of small renal vessels increased with the age of nzb/w f1 mice. the wheat germ agglutinin bound to structures with alkaline phosphatase activity.	1993	8351979
neurons in a variety of molluscs react to antibodies raised against the vd1/rpd2 alpha-neuropeptide of the pond snail lymnaea stagnalis.	the vd1 and rpd2 neurons of lymnaea stagnalis innervate other central neurons, certain skin areas, the pneumostome area, and the auricle of the heart. recently, a set of four (delta, epsilon, alpha, beta) neuropeptides produced by these giant neurons and by certain other central neurons has been characterized. although alternative splicing of the preprohormone of these neurons yields at least 10 different alpha neuropeptides, an affinity-purified antiserum directed against a domain common to all ...	1993	8364974
fragmentation and dispersal of golgi proteins and redistribution of glycoproteins and glycolipids processed through the golgi apparatus after infection with herpes simplex virus 1.	in vero monkey cells and hep-2 human epidermoid carcinoma cells infected with herpes simplex virus 1 the proteins beta-cop, galactosyltransferase, and alpha-mannosidase ii associated with the golgi apparatus appear to be associated with numerous smaller structures dispersed throughout the cytoplasm. concomitantly, the intracytoplasmic ligands of lectins normally associated wholly (helix pomatia or ricinus communis agglutinin) or in part (wheat germ agglutinin) with the golgi apparatus increased ...	1993	8385343
immune-mediated alteration of the terminal sugars of goblet cell mucins in the small intestine of nippostrongylus brasiliensis-infected rats.	alteration of terminal sugars of goblet cell mucins in the small intestinal mucosa was examined by lectin histochemistry in rats infected with nippostrongylus brasiliensis. to see whether alteration of the nature of mucins was regulated by the local immune system, adult worms were implanted intraduodenally into recipient rats of various immune status. when 7-day-old ('normal') adult worms were implanted into naive euthymic rats, about 60% remained until day 7 and then were expelled. the number o ...	1993	8473019
cytochemical characterization of basement membranes in the enamel organ of the rat incisor.	ameloblasts are unique epithelial cells, in that once they have deposited the entire thickness of enamel and the process of maturation begins, they reform a basal lamina-like structure at their apical surface. in order to characterize further this basal lamina, its composition was analysed using (1) lectin-gold cytochemistry for glycoconjugates, (2) high-iron diamine (hid) staining for sulfated glycoconjugates and (3) immunogold labeling for collagen type iv and laminin. the labeling patterns we ...	1993	8500995
prognostic significance of helix pomatia lectin and c-erbb-2 oncoprotein in human breast cancer.	we investigated the prognostic significance of helix pomatia lectin (hpa) staining on disease-free and overall survival in 120 primary breast carcinomas. hpa staining was present in 58 (48%) of these carcinomas. it was significantly associated with axillary lymph node metastases (p < 0.001) and c-erbb-2 expression (p < 0.01). a univariate study revealed that disease-free and overall survival were significantly correlated with clinical stage, tumour size, axillary lymph node metastases. hpa stain ...	1993	8102537
immunocytochemical distribution of neuropeptide f (npf) in the gastropod mollusc, helix aspersa, and in several other invertebrates.	the distribution of neuropeptide f (npf) immunoreactivity in the snail, helix aspersa, has been demonstrated by immunocytochemistry using 2 region-specific antisera. one, designated npf3, was raised against a synthetic n-terminal fragment of helix aspersa npf; the other, designated pp221, was raised against the c-terminal hexapeptide amide of mammalian pancreatic polypeptide (pp) but cross-reacts fully with the analogous c-terminal region of helix aspersa npf. the distribution of npf immunoreact ...	1994	8111844
identification of a major poly-n-acetyllactosamine-containing cell-surface glycoprotein of mouse teratocarcinoma cells. appearance on cells induced to primitive endoderm but not parietal endoderm differentiation.	mouse teratocarcinoma f9 cells were induced to primitive endoderm differentiation with retinoic acid, and poly-n-acetyllactosamine-containing surface glycoproteins were identified by radiolabelling endo-beta-galactosidase-cleavable glycans with galactosyltransferase and radiolabelled udp-galactose. one major radiolabelled band with an apparent size of 250-500 kda was identified which differed from the known poly-n-acetyllactosamine-containing glycoproteins laminin, fibronectin, lysosome-associat ...	1994	8125095
helix pomatia agglutinin binding in human tumour cell lines: correlation with pulmonary metastases in nude mice.	the extent of lectin binding by three human melanoma (lox, femx-1 and sesx) and two sarcoma lines (mhmx and ohsx) was related to their potential for experimental metastasis formation in athymic nude mice. the helix pomatia agglutinin (hpa), which recognises the n-acetyl-d-galactosamine ligand, showed differential binding to the cell lines in a manner that correlated with their ability to give lung colonies after i.v. injection in the mice (p < 0.005). the degree of hpa binding and lung colony fo ...	1994	8198963
radioimmunoassay of 2-hydroxyestrone in urine.	catechol estrogens such as 2-oh estrone are interesting estrogen metabolites formed in several human tissues and excreted in urine. we developed and thoroughly validated a radioimmunoassay for urinary 2-oh estrone that has several advantages over published rias. because we developed a relatively specific antiserum, we did not include a preliminary chromatographic step to eliminate cross-reacting urinary steroids. we hydrolyzed urinary steroid conjugates with beta-glucuronidase from helix pomatia ...	1994	8287549
characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the rer to the golgi complex requires only one vesicular transport step.	mouse hepatitis coronavirus (mhv) buds into pleomorphic membrane structures with features expected of the intermediate compartment between the er and the golgi complex. here, we characterize the mhv budding compartment in more detail in mouse l cells using streptolysin o (slo) permeabilization which allowed us to better visualize the membrane structures at the er-golgi boundary. the mhv budding compartment shares membrane continuities with the rough er as well as with cisternal elements on one s ...	1994	8294506
heterogeneity of human gallbladder mucin in bile.	1. human gallbladder mucin has been implicated to play a role in gallstone disease. in spite of this fact relatively little is known about the structure of human gallbladder mucin. in this study we have investigated the possible heterogeneity of mucin. for this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. all antibodies reacted primarily with carbohydrate antigenic determinants. with these antibodies the immunoreactivity of gallbladder mucin from 60 patient ...	1994	8306554
carbohydrate antigens of human megakaryocytes and platelet glycoproteins: a comparative study.	until now, carbohydrate antigens of human megakaryocytes have not been studied very extensively. for this reason, we investigated the staining pattern of 25 lectins and carbohydrate-specific monoclonal antibodies on paraffin-embedded trephine biopsies and acetone-fixed smears from patients with reactive and neoplastic bone marrow lesions. a biotin-streptavidin-alkaline phosphatase assay was used to visualize the binding of lectins or antibodies. ulex europaeus agglutinin i (uea-i) stained megaka ...	1994	7532635
studies on steroids in fetuses and neonates: identification of 16-dehydropregnenolone in the circulation of pre-term neonates.	unconjugated steroids were extracted with dichloromethane from the serum of pre-term neonates. conjugated steroid fraction in the residue was separated and purified by high performance liquid chromatography. the purified steroid fraction was subjected to enzyme (arylsulfatase from helix pomatia) or acid hydrolysis. liberated steroid was analyzed by gas chromatography-mass spectrometry (gc/ms) and gas chromatography-fourier transformation infrared spectroscopy (gc/ft-ir). all of the results obtai ...	1994	7703972
histochemical characteristics of the goblet cell mucins and their role in defence mechanisms against nippostrongylus brasiliensis infection in the small intestine of mice.	the histochemical characteristics of the small intestinal goblet cell mucins of mice and their protective role during the expulsion of nippostrongylus brasiliensis adult worms were examined. lectin histochemistry revealed that, before infection, strongly positive stainability of helix pomatia agglutinin (hpa) and limax flavus agglutinin (lfa) was observed in all of the small intestinal goblet cell mucins of mice. when 'normal' or 'damaged' n. brasiliensis adult worms were implanted separately in ...	1994	7708431
[identification of group c streptococcus with helix pomatia lectin in use for blood grouping].	the lectin anti-ahp (helix lectin) obtained from the albumen gland of an edible snail, helix pomatia, was used for the identification of group c streptococci. all 1,045 strains of group c streptococci (s. equisimilis, s. zooepidemicus, and s. dysgalactiae) were agglutinated with the helix lectin, but none of the 12,264 strains of group a, 1,346 strains of group g, 330 strains of group b, and 121 strains of other streptococcal groups was not. a few strains of s. equisimilis which belongs to group ...	1994	7830321
biliary proteins from hepatic bile of rats fed curcumin or capsaicin inhibit cholesterol crystal nucleation in supersaturated model bile.	following our earlier observations that curcumin and capsaicin are antilithogenic in mice and hamsters, attempts were now made to understand the manner in which these spice principles were acting. for this purpose, the hepatic biles of rats fed a control, lithogenic, and lithogenic diet supplemented with curcumin or capsaicin were subjected to gel filtration chromatography (sepharose-4b-cl) and the lmw protein fractions were tested for their ability to influence cholesterol crystal growth in mod ...	1994	7851942
optimal enzymatic hydrolysis of urinary benzodiazepine conjugates.	conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. optimal recovery of the free drugs occurs when 1 ml of urine buffered to ph 4.5 is incubated with 5000 u of helix pomatia beta-glucuronidase at 56 degrees c for 2 h. urine specimens containing conjugates of temazepam, oxazepam, lorazepam, alpha-hydroxyalprazolam, 2-hydroxyethylflurazepam, and n-desalkyl-3-hydroxyflurazepam as targeted benzodiazepines were used. the freed drugs were quantitated by g ...	1994	7861750
[the human spleen in idiopathic thrombocytopenic purpura].	idiopathic (autoimmune) thrombocytopenic purpura (itp, aitp) represents a relatively frequent impairment. it involves a syndrome of various diseases with a shortened thrombocytes survival caused by anti-platelet antibodies. the majority of cases are of secondary character. spleenectomy often evokes a complete remission of thrombocytopenia. the study describes morphologic findings in spleens of 30 patients with the clinical diagnosis of itp/aitp. the findings were gained by light microscopy from ...	1994	7882065
modes of entry of the first-stage larvae of elaphostrongylus cervi (nematoda: protostrongylidae) into pulmonate snails arianta arbustorum and helix pomatia.	experimental infection of the pulmonate snails arianta arbustorum l. and helix pomatia l. with first-stage larvae of protostrongylid nematode elaphostrongylus cervi cameron, 1931 was performed in order to determine modes of larval entry into the body of the snail intermediate host. groups by four individuals of both snail species were examined histologically 30, 60, 90, and 120 minutes after the beginning of exposure and 1, 2, 4, and 7 days post infection. all 64 snails examined were found to be ...	1994	7883253
lectin binding in the anterior segment of the bovine eye.	eleven different fluorescent lectin-conjugates were used to reveal the location of carbohydrate residues in frozen sections of the anterior segment of bovine eyes. the lectins were specific for the following five major carbohydrate groups: (1) glucose/mannose group (concanavalin a (con a)); (2) n-acetylglucosamine group (wheat germ agglutinin (wga)); (3) galactose/n-acetylgalactosamine group (dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), helix aspersa agglutinin (haa), psop ...	1994	7883589
interactions with lectins and agglutination profiles of clinical, food, and environmental isolates of listeria.	on the basis of preliminary trials with 14 collection strains of listeria, five lectins (canavalia ensiformis, concanavalin a; griffonia simplicifolia lectin i; helix pomatia agglutinin; ricinus communis agglutinin; and triticum vulgaris wheat germ agglutinin) were selected to set up a microtiter agglutination assay. the lectin agglutination profiles of 174 clinical, food, and environmental strains of listeria monocytogenes, listeria innocua, and listeria seeligeri were investigated. data on the ...	1994	7883879
hyperplasia, hypertrophy, and phenotypic alterations in the distal nephron after acute proximal tubular injury in the rat.	little is known about the impact of acute proximal tubular injury and dysfunction on the distal nephron.	1994	7909858
glycosylation patterns of the human colon cancer cell line ht-29 detected by helix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in scid mice.	human colonic cancer cells (ht-29, 10(7) cells/dose) were injected subcutaneously between the scapulae of 19 severe combined immunodeficient (scid) mice. after 19 days, large tumours had developed in 18 out of the 19 animals and the mice were then killed. metastases were detected in the lungs of 16 animals but not in other organs investigated. surgical removal of the primary tumour in another group of five animals led to a prolonged survival and further growth of metastases in the lungs. ht-29 i ...	1994	7923992
role of cell surface o-linked oligosaccharides in adhesion of hl60 cells to fibronectin: regulation of integrin-dependent cell adhesion by o-linked oligosaccharide elongation.	the adhesion of the myelogenous leukemia cell line, hl60, to fibronectin and its fragments, heparin binding fragment (40 kda) and cell attachment fragment (120 kda), was enhanced by culturing with benzyl-alpha-galnac (bz alpha galnac). enhancement of cell adhesion to fibronectin was also observed on treatment of hl60 cells with 12-o-tetradecanoylphorbol 13-acetate (tpa). however, an additive effect of bz alpha galnac and tpa treatments was not observed. the expression of vla4 and vla5 did not ch ...	1994	7925647
comparative aspects of neoplastic invasion of the brain.	this review of the invasion of the human brain by tumors is based upon a comparative approach. the taxonomic distribution of a structure of the cns to be considered a brain is present in the following taxonomic groups: mollusca (cephalopoda, pulmonata) sipunculida, echiurida, annelida, arthropoda and vertebrata (fishes, amphibians, reptiles, birds and mammals). the comparative approach (inter-, and intraspecies specific) provides a more informative, indicative, understanding of invasion of the h ...	1994	7964978
helix pomatia agglutinin binding is a useful prognostic indicator in colorectal carcinoma.	most deaths from colorectal carcinoma are due to metastases. a relatively reliable prognostic indicator at surgery to date is the dukes' stage, but this is a morphologic approach that does not elucidate biochemical changes to explain why cells became metastatic. the binding sites for the lectin from the roman snail helix pomatia (hpa) were shown to be good prognostic indicators in breast and gastric cancer, and accordingly, this study was performed to evaluate the use of hpa binding sites as pro ...	1994	7982174
galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study.	this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome.	1994	8001826
differential lectin binding of onchocerca lienalis and onchocerca ochengi infective larvae following their development in simulium ornatum s.l.	lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two onchocerca species following their development in simulium ornatum. of the seven fitc-labelled lectins used in this study only two, arachis hypogea (pna) and helix pomatia (hpa), bound to the surface of o. lienalis, whereas no lectin binding could be detected on the surface of o. ochengi infective larvae. this indicates that in principal lectins might be a pot ...	1994	8066375
alpha-n-acetylgalactosaminidase deficiency with mild clinical manifestations and difficult biochemical diagnosis.	two additional patients with alpha-n-acetylgalactosaminidase (alpha-naga) deficiency are described. an 11-month-old girl with nonconsanguineous parents had generalized seizures and no angiokeratoma. biochemical investigation showed persistent slight oligosacchariduria; enzymatic analysis of plasma, leukocytes, and fibroblasts revealed profound alpha-naga deficiency. heterozygote enzyme levels were found in both parents. the mother has epilepsy, and epilepsy is present in the father's family. a y ...	1994	8071745
the use of lectins to characterize and separate living canine spermatozoa: a preliminary study.	this study was designed as a preliminary attempt to develop a methodology for relating the glucidic structure of the sperm membrane to sperm morphology. differences in plasma membrane glycoconjugates between motile and nonmotile spermatozoa were studied by using 7 lectins. fresh spermatozoa from 3 dogs were analyzed by fluorescein isothiocyanate (fitc) labeled lectins. the binding of lectins to the sperm membrane and the capability of the lectins to agglutinate spermatozoa were estimated semi-qu ...	1994	16727393
interaction of hamster submaxillary sialyl-tn and tn glycoproteins with gal, galnac and glcnac specific lectins.	hamster submaxillary glycoprotein (hsm), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. the thr and ser residues in the protein core account for more than half of all of the amino acid residues, while lys, glu, pro and ala are the major components of the remaining portion of amino acids. the carbohydrate side chains of this mucous glycoprotein have mainly the neuac-galnac-(sialyl-tn) seque ...	1994	8183285
interaction of a novel tn (galnac alpha 1-->ser/thr) glycoprotein with gal, galnac and glcnac specific lectins.	a naturally occurring tn glycoprotein (native asg-tn) with galnac alpha 1-->ser/thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. in a quantitative precipitin assay, this glycoprotein completely precipitated maclura pomifera (mpa), vicia villosa b4 (vvl-b4) and artocarpus integrifolia (jacalin, ail). it also reacted well with helix pomatia (hpl) and wistaria floribunda (wfl) and precipitated over 75% of the lectin nitrogen added, but poorly with ric ...	1994	8292029
growth hormone regulates an n-acetylgalactosamine component in odontogenesis: a specific lectin-binding study in the lewis dwarf rat.	dental organs of incisors from normal, dwarf and growth hormone-treated dwarf rats were analysed histochemically using a panel of lectins. a distinctive pattern of differential staining was obtained with helix pomatia agglutinin, a lectin specific for n-acetylgalactosamine. in bouin's perfused and paraffin-embedded undecalcified tissues from normal rats, reaction product for n-acetylgalactosamine was visible in the odontogenic cells and some extracellular matrices. in the growth hormone-deficien ...	1994	7519266
nm23 expression versus helix-pomatia lectin-binding in human breast-cancer metastases.	the relationship between nm23 expression, hpa staining, and other clinicopathological variables, i.e., axillary (ax) and internal mammary lymph node (imn) metastases was assessed, and their prognostic values were analyzed. positive hpa staining was found to be significantly correlated with tumor size, and with ax and imn metastases, whereas negative nm23 expression was significantly correlated with ax and imn metastases. a univariate study revealed that overall survival was correlated significan ...	1994	21567061
detection of dihydrotestosterone (dht) doping: alterations in the steroid profile and reference ranges for dht and its 5 alpha-metabolites.	dihydrotestosterone (dht), a biologically active metabolite of testosterone, may be misused in sports to benefit from its anabolic and psychotropic effects. after dht application, a significant increase of the glucuronides of dht and its metabolites can be expected for a certain time period depending upon dose, formulation, route of administration, and in case of percutaneous administration the chainlength of the ester. dht and its metabolites can be monitored by gas-chromatography/mass spectrom ...	1995	8776070
tachykinin- and leucokinin-related peptides in the molluscan nervous system.	distribution of locustatachykinin-like immunoreactive (lomtkli) and leucokinin like immunoreactive (lkli) neurons in the central nervous system (cns) of the pond snail, lymnaea stagnalis was investigated and compared to that found in helix pomatia. occurrence of lomtkli neurons in different ganglia of the freshwater bivalve, anodonta cygnea, was also studied. similar to helix, the lymnaea cns contained lomtkli and lkli neurons mainly in the cerebral and pedal ganglia, but the number of labelled ...	1995	8853699
distribution of catch-relaxing peptide (carp)-like immunoreactive neurons in the central and peripheral nervous system of helix pomatia.	immunocytochemistry was performed on the nervous system of helix by the use of an antibody raised against a myotropic neuropeptide, the catch-relaxing peptide (carp), isolated from mytilus edulis. in each ganglion of the central nervous system of helix pomatia, numerous carp-immunoreactive cell bodies and a dense immunoreactive fiber system could be observed with a dominancy in the cerebral and pedal ganglia. the majority of the immunoreactive neurons are unipolar, although multipolar neurons al ...	1995	7781031
neurons with different immunoreactivity form clusters in the cns of helix pomatia.	the distribution of serotonin (5ht-ir), fmrf amide (fmrf a-ir), catch-relaxing-peptide (carp-ir), dopamine (da-ir), gamma-amino-butyric-acid (gaba-ir), and leucokinin (lk-ir) immunoreactive neurons were compared in the ganglia of helix cns. these neurons are not distributed randomly, but their location outlines distinct groups in the ganglia. in a few groups only da-ir, gaba-ir and lk-ir neurons can be seen, whereas in the majority of groups fmrfa-ir, carp-ir and 5ht-ir neurons are localized tog ...	1995	8853698
glycoconjugates and keratin 18 define subsets of taste cells.	sections of neonatal, normal adult and denervated adult rat tongue were examined with lectin histochemistry. attention was focused upon intragemmal cells (cells within the taste bud) and the surrounding perigemmal cells. informative staining patterns were observed with four of 12 lectins: ulex europaeus (uea-i), bauhinia purpurea (bpa), helix pomatia (hpa) and lotus tetragonolobus (lta) agglutinins. in normal adult tongues, bpa bound to those lingual epithelial cells lacking contact with the bas ...	1995	8789401
the human muc2 mucin apoprotein appears to dimerize before o-glycosylation and shares epitopes with the 'insoluble' mucin of rat small intestine.	rabbit antiserum against a synthetic peptide corresponding to a tandemly repeated amino acid sequence in the human intestinal mucin apoprotein muc2 was used in immunoprecipitation to study the biosynthesis of muc2 in the colon-carcinoma cell line ls 174t. under non-reducing conditions, two bands were precipitated, the smaller with an apparent size of about 700 kda on sds/page. when analysed by two-dimensional electrophoresis after reduction, the larger band migrated to the same position as the s ...	1995	8948445
lectin-binding properties of human breast cancer cell lines and human milk with particular reference to helix pomatia agglutinin.	several studies have shown binding of a variety of lectins to breast cancer cells in tissue sections. in particular, binding of the lectin from the roman snail, helix pomatia agglutinin (hpa), to breast cancer cells is linked with a poor prognosis. the molecular basis for lectin binding to metastatic breast cancers is not known. to elucidate this in a model system, lectin-binding patterns of seven human breast cancer cell lines were investigated, their cell membranes were isolated, and hpa bindi ...	1995	7868857
identification of a sperm penetration factor in the oviduct of the golden hamster.	previously, we found oviductal eggs to be significantly more penetrable and fertilizable in vitro than ovulated eggs collected from the ovarian bursa, while bursal eggs were comparable to mature (unovulated) follicular eggs. incubation of follicular eggs with a soluble eluate of oviductal egg cumulus complexes (cof) increased sperm penetration: the activity was macromolecular, was destroyed at 56 degrees c, and was produced in the oviduct. we now report purification of this oviductal factor that ...	1995	7711179
identification of a novel glycosaminoglycan core-like molecule. ii. alpha-galnac-capped xylosides can be made by many cell types.	the accompanying article (manzi, a., salimath, p. v., spiro, r. c., keifer, p. a., and freeze, h. h. (1995) j. biol. chem. 270, 9154-9163) reported the complete structure of a novel molecule made by human melanoma cells incubated with 1 mm 4-methylumbelliferyl-beta xyl (xyl beta mu). the product resembles a common pentasaccharide core region found in chondroitin/dermatan sulfate glycosaminoglycans, except that a terminal alpha-gal-nac residue is found in a location normally occupied by beta-galn ...	1995	7721831
a novel cdna sequence encoding the precursor of the d-amino acid-containing neuropeptide fulicin and multiple alpha-amidated neuropeptides from achatina fulica.	fulicin (phe-d-asn-glu-phe-val-nh2) is an endogenous neuropeptide containing a d-amino acid from ganglia of the african giant snail achatina fulica. we have cloned a novel cdna (1,995 nucleotides) encoding a fulicin precursor from the snail cerebral and subesophageal ganglia. the fulicin precursor protein (357 amino acids) contains one copy of fulicin and at least nine other putative alpha-amidated neuropeptides composed of four to six amino acid residues. seven of the nine neuropeptides were no ...	1995	7722509
native and asialo-tamm-horsfall glycoproteins as important ligands for the detection of galnac beta 1-->and gal beta 1-->4glcnac active lectins.	the binding properties of human tamm-horsfall sd(a+) urinary glycoprotein(thgp) and asialo-thgp with various applied lectins was investigated by quantitative precipitin and precipitin inhibition assays. both glycoproteins completely precipitated abrus precatorius agglutinin(apa). they also reacted well with wistaria floribunda (wfa), glycine max (soybean, sba), and ricinus communis agglutinins and precipitated over 78% of the lectin nitrogen added, but reacted poorly or weakly with all alpha-ano ...	1995	7726822
ultrastructural localization of helix pomatia agglutinin (hpa)-binding sites in human breast cancer cell lines and characterization of hpa-binding glycoproteins by western blotting.	ultrastructurally, cells of five human breast cancer cell lines (mcf7, bt549, bt20, t47d, and hbl100) generally displayed many characteristics of their epithelial origin. the most distinctive features were observed in mcf7 cells, which consistently showed microvilli and submembranous granules. these ultrastructural findings served as a basis for localizing the binding sites of the lectin helix pomatia agglutinin (hpa). after use of the pre-embedding method consistent hpa labeling of the cell mem ...	1995	7770962
histochemical study of lectin binding in the human fetal minor salivary glands.	the emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. an avidin-biotin technique was used to study the binding of lectins from ulex europeus i (uea-i), dolichos biflorus (dba), glycine maximus (sba), helix pomatia (hpa), arachis hypogaea (pna) and triticum vulgare (wga) to specific sugars on sections of tissue from labial glands, glands of blandin and nuhn, glands ...	1995	7776265
use of sialylated or sulfated derivatives and acrylamide copolymers of gal beta 1,3galnac alpha- and galnac alpha- to determine the specificities of blood group t- and tn-specific lectins and the copolymers to measure anti-t and anti-tn antibody levels in cancer patients.	sialylated or sulfated derivatives and acrylamide copolymers of blood group t-(gal beta 1,3galnac alpha-) and tn-(galnac alpha) haptens were studied for their interaction with the lectins of peanut (pna), agaricus bisporus-(aba), helix pomatia-(hpa) and vicia villosa b4-(vva), using asialo cowper's gland mucin (acgm), which contains both t and tn epitopes, as the coating substrate in enzyme linked lectin assay. both t and tn copolymers (-40 haptens) showed high affinity and strict specificity; a ...	1995	7795413
monoclonal antibody recognizing a determinant on type 2 chain blood group a and b oligosaccharides detects oncodevelopmental changes in azoxymethane-induced rat colon tumors and human colon cancer cell lines.	altered expression of abh blood group substances is a common feature of human colorectal carcinoma, yet it remains unclear how these structural changes influence the biological properties of tumor cells. azoxymethane-induced rat colon tumors display many features of the human disease, thereby providing a potentially useful model to study the role of blood group substances in colon cancer progression. we have prepared monoclonal antibodies to a microsomal fraction isolated from an azoxymethane-in ...	1995	7536750
lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome.	aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ...	1995	7542024
comparative effects on biliary concanavalin a-bound glycoproteins and calcium ion on cholesterol crystal nucleation and growth in model bile.	the concanavalin a-bound glycoproteins in human gallbladder bile have recently been demonstrated to be strong promoters of cholesterol crystal nucleation. in the present study, we investigated the mechanism(s) whereby such promoters affect cholesterol crystal nucleation and/or growth, and compared these mechanisms with those of another promoter, calcium ion. concanavalin a-bound glycoproteins were isolated from the helix pomatia-unbound fraction of gallbladder bile from stone-free patients, and ...	1995	7550861
comparison of the hydrolysis rates of morphine-3-glucuronide and morphine-6-glucuronide with acid and beta-glucuronidase.	the acid and enzymatic hydrolysis rates for two morphine metabolites, morphine-3-glucuronide (m3g) and morphine-6-glucuronide (m6g), were compared. urine fortified with m3g or m6g was hydrolyzed in a boiling water bath or with beta-glucuronidases. optimal temperatures for beta-glucuronidases were 60 degrees c for helix pomatia and abalone and 50 degrees c for escherichia coli. a 3-h incubation with 1,000-1,500 fishman units of enzyme gave complete hydrolysis of m3g. the enzymatic hydrolysis rate ...	1995	7564293
purification of murine pulmonary type ii cells for flow cytometric cell cycle analysis.	mice are widely used as animal models for in vivo lung disease. despite this fact, few methods exist for isolation of type ii pneumocytes from mouse lung, limiting the study of alveolar epithelial characteristics in these models. this study investigated several methods for labeling murine lung cell suspensions for flow cytometric identification and sorting of type ii pneumocytes. crude lung cell suspensions were prepared after intratracheal instillation of dispase and were labeled using phosphin ...	1995	7621777
lectins inhibit the aujeszky's disease virus-induced interferon-alpha production of porcine peripheral blood mononuclear cells.	the interaction between virus and peripheral blood mononuclear cells (pbmc) required to elicit the production of interferon-alpha (ifn-alpha) by the so-called natural interferon-producing cell is unknown. however, results from inhibition experiments suggest that viral glycoproteins are essential in this ifn induction process. we demonstrate here that cellular glycoproteins also appear to be involved in the initiation of ifn-alpha production. lectins, that is, sugar binding glycoproteins, inhibit ...	1995	7627804
isolation of iga1 from human serum by affinity chromatography using an immobilized extract of the albumin gland of helix pomatia.	an extract of the albumin gland of helix pomatia was linked to sepharose-4b and used to prepare iga from group o human serum; immunoelectrophoresis showed that the preparation was free of igg and igm. from studies with specific iga subclass antisera and by comparison with the activity of jacalin-produced material the helix pomatia extract was found to be iga1 specific. the preparation had red cell anti-a, b specificity and was suitable for standardizing and controlling anti-human iga reagents. p ...	1995	7655574
comparison of procedures for measuring the quaternary n-glucuronides of amitriptyline and diphenhydramine in human urine with and without hydrolysis.	the activities of beta-glucuronidases from helix pomatia, escherichia coli and rat towards the n-glucuronides of amitriptyline and diphenhydramine were considerably lower than those towards standard substrates. the two n-glucuronides were analysed in urine samples by the following procedures: hplc of the intact conjugate after solid-phase extraction on a cation exchanger cartridge or after direct injection of urine; hplc of the aglycone after hydrolysis with beta-glucuronidase from h. pomatia or ...	1995	7674140
structure and genetic polymorphism of blood group a-active glycosphingolipids of the rat large intestine.	study of blood group a- and b-active glycosphingolipid content of the epithelium of the large intestine of 16 strains of inbred rats led to the discovery of two related strains, shr and wky, devoid of a-active glycolipids, whereas all strains expressed b-active glycolipids. this finding evidenced a new a/non-a genetic polymorphism in the rat. blood group a-active glycolipids were isolated from the large intestine of f344 rats and purified by affinity chromatography on immobilized helix pomatia l ...	1995	7696327