Publications

[effects of mebendazole and cambendazole on enterohelminths in pigs].	mebendazole, administered at a dose of 30 mg active substance per 1 kg of feed, was found to have 100% effectiveness on ascaris suum and cambendazole, administered at a dose of 1.5 g per 1 kg of liver weight, showed the same effectiveness in the control of ascaris suum and oesophagostomum dentatum. the effectiveness of both drugs on trichocephalus suis and strongyloides ransomi was low. mebendazole and cambendazole can be recommended for mass dehelminthization of pigs in affected stocks. helmint ...	1979	111399
airway sensitivity to slow-reacting substance of anaphylaxis, histamine, and antigen in ascaris sensitive monkeys.	the effects of ascaris suum antigen, histamine, and slow-reacting substance of anaphylaxis (srs-a) on the respiratory system were compared in 3 anesthetized rhesus monkeys. the agents were administered by instillation into the trachea, and the animals were studied in a volume displacement body plethysmograph. two of the animals showed skin and bronchial sensitivity to ascaris suum antigen and responded to it with increased pulmonary resistance and decreased dynamic compliance. a similar response ...	1979	36017
ascaris suum: electrophoretic characterization of reproductive tract and perienteric fluid polypeptides, and effects of seminal and uterine fluids on spermiogenesis.		1979	36284
in vitro activation and behavior of the ameboid sperm of ascaris suum (nematoda).	a system is described for the study of activation and motility of ascaris spermatozoa in vitro. activation was accomplished by addition of the sperm-activating substances (sas), extracted from the male accessory gland, to cells incubated in phosphate-buffered saline (ph 7.4) at 37-39 degrees c under anaerobic conditions (95% n2, 5% co2). activation is characterized by a change from spherical to ameboid shape with coalescence of the refringent granules. the normal ameboid spermatozoa bear several ...	1979	39678
isolation and some properties of an acid protease from fasciola hepatica.	a new protease, detected in an extract of fasciola hepatica, was isolated and partly purified. the ph optimum for the cleavage of denaturated haemoglobin by the enzyme is ph 3.0. this proteolytic activity is inhibited by diazoacetylnorleucine methyl ester, pepstatin, the pepsin inhibitor from ascaris suum, and phenylalanine. the cathepsin d inhibitor from potatoes, edta, mercaptoethanol and the inorganic salts tested have no inhibitory effect. the cleavage of the b-chain of oxidized insulin by e ...	1979	44401
[changes in resistance by stress and feeding. ii. effect of isoenergetic fat diets on resistance of rats against ascaris suum infections].		1979	395799
comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (e.l.i.s.a.).	112 sera from europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (e.l.i.s.a.) against 6 crude extracts of various helminths (2 of adult worms: dipetalonema viteae, fasciola hepatica; 3 of eggs: ascaris suum, toxocara canis, schistosoma mansoni; and of echinococcus granulosus scolices) and against bovine hydatid fluid. each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. extensive cross-reactions were obser ...	1979	92420
airway responses to histamine and methacholine in ascaris suum-allergic rhesus monkeys.		1979	118144
effect of pyrantel tartrate and carbadox on acquisition of the swine kidneyworm (stephanurus dentatus) and other parasites by pigs on contaminated lots.	a combination of pyrantel tartrate (106 mg/kg of body weight) and carbadox (55 mg/kg of body weight) in ground feed was fed to 20 weaned pigs (av wt, 14.4 kg) for 42 days. another group of 20 pigs included nontreated controls. the pigs were farrowed and suckled in a slat-floored farrowing house and had minimal exposure to the small intestinal threadworm (stronglyoides ransomi) until they were placed on severely contaminated dirt lots at the start of the experiment. five pigs from each of the two ...	1979	160763
ascaris suum: suppression of reaginic and hemagglutinating antibody responses in the mouse by crude extract and maintenance fluid.		1979	437015
the role of upper airways in antigen-induced bronchoconstriction.	the bronchoconstrictor effect of antigen was tested in 15 dogs. the upper and the lower airways were sequently exposed through a specially confected endotracheal tube. the antigen was also generally administered through a mouth mask. bronchoconstriction was induced after each kind of exposure. the results argue for reflex bronchoconstriction. the sensory receptors localized in the upper part of the airways are responsible for about 50% of antigen-induced bronchoconstriction. the decrease of po2a ...	1979	441542
dose titration of oxfendazole against common nematodes of swine.	oxfendazole, a benzimidazole carbamate, was administered as a 0.5% feed additive to 88 pigs naturally infected with two to four nematode species. dose rates of 1.5 mg/kg of body weight, 3.0 mg/kg, 3.75 mg/kg, 4.5 mg/kg, 6.75 mg/kg, or 9.0 mg/kg were 100% efficacious against oesophagostomum dentatum and 99.2% to 100% effective against ascaris suum. dose rates of 4.5 mg/kg, 6.75 mg/kg, and 9.0 mg/kg were 92.7%, 98.9%, and 99.5% effective, respectively, against mixed populations of metastrongylus a ...	1979	464370
esophagogastric ulcers in swine infected with ascaris suum.		1979	473495
the metabolism of the anthelmintics clioxanide and resorantel and related compounds in vitro by moniezia expansa, ascaris suum and mouse- and sheep-liver enzymes.	1. clioxanide and related compounds were deacetylated by the cestode moniezia expansa, the nematode ascaris suum, by enzymes prepared from these species and by mouse- and sheep-liver homogenates. deacetylase activity was found in the cytosol of cestode proglottids throughout the strobila, and in the cytosol of nematode intestinal cells and reproductive tract. 2. the o-deacetylases from both helminths showed similar ph optima of about 7.0. activity was enhanced by ca2+ and low molecular weight th ...	1979	483862
the isolation and partial characterization of a protective antigen from developing larvae of ascaris suum.		1979	489237
effect of ascaris suum and other adjuvants on the potentiation of the ige response in guinea-pigs.	the potentiation effect of various adjuvants on the production of guinea-pig ige was investigated using freund's complete and incomplete adjuvant, the lipopolysaccharide of escherichia coli and salmonella typhosa, bordetella pertussis, and the nematodes nippostrongylus brasiliensis and ascaris suum. while all the antigens had a variable effect on the potential of the igg response, only infection with a. suum resulted in an enhanced ige response to the antigen, egg albumin. maximum potentiation o ...	1979	500118
satellite dna properties of the germ line limited dna and the organization of the somatic genomes in the nematodes ascaris suum and parascaris equorum.	during the early cleavage divisions in some ascarids, parts of the chromosomes are eliminated from the somatic blastomeres ("chromatin diminution", boveri, 1887) while the chromosomes in the germ line cells maintain their integrity. to characterize the germ line and soma genome, dna was isolated from gametes and embryonic somatic cells of two ascarid species, parascaris equorum var. univalens and ascaris suum. it was shown that the germ line limited dnas of these species have the same density an ...	1979	510087
immunopathology of toxocara canis and ascaris suum infections of the eye: the role of the eosinophil.	intravitreal infection of guinea pigs with second-stage larvae of toxocara canis and second- and fourth-stage larvae of ascaris suum induced intraocular ige antibodies and a dense eosinophil infiltration in the anterior chamber and throughout the uveal tract. the eosinophil infiltrate began within 1 day after infection and persisted for as long as 51 days. by day 12 after intravitreal infection, the injected ascarid larvae were surrounded by granulomas which consisted almost entirely of eosinoph ...	1979	511459
the metabolism of nitrophenolic and 5-arylazorhodanine anthelmintics by ascaris suum, moniezia expansa and by mouse- and sheep-liver enzymes.	1. the anthelmintics disophenol (2,6-diiodo-4-nitrophenol), nitroxynil (3-iodo-4-hydroxy-5-nitrobenzonitrile) and nitrodan (3-methyl-5-(4-nitrophenylazo)rhodanine) were reduced in vitro to the corresponding amines by intact ascaris suum, moniezia expansa, by enzymes prepared from these helminths, and by mouse- and sheep-liver homogenates. helminth reductases required nadh2 and glutathione as cofactors and were inhibited about 50% by 2.0 x 10(-7) m allopurinol. azo bonds of nitrodan and its analo ...	1979	516789
proteolytic enzymes and enzyme inhibitors in ascaris suum. iv. estimation of molecular weights of chymotrypsin inhibitors and an intestinal protease by gel chromatography.		1979	517354
proteolytic enzymes and enzyme inhibitors in ascaris suum. v. influence of parasite gut content on host amylase and lipase.		1979	517355
chromosome diminution in ascaris suum. two-fold increase of nucleosomal histone to dna ratios during development.	the swine intestinal nematode, ascaris suum, eliminates chromatin material from its primordial somatic cells during early embryogenesis. a technique for isolation of nuclei from pre- and post-diminution stage embryos has been developed and these isolated nuclei were used in investigations of nuclear events during diminution. the amount of dna per nucleus determined by diphenylamine assays and isotope dilutions was 0.66 pg and 0.29 pg in pre- and post-diminution nuclei, respectively. thus, a. suu ...	1979	518883
ige and igg1 antibody production by a soluble product of ascaris suum in the guinea-pig.	third-stage larvae of ascaris suum cultured to the fourth stage in a chemically defined culture medium produced a substance, the 'acf antigen', which was allergenic in the guinea-pig. when three different concentrations (3.1, 31 and 62 micrograms) of the acf antigen were given intraperitoneally, only the highest concentration induced a primary ige specific antibody response (1:100 titre) as determined with the passive cutaneous anaphylaxis reaction. upon secondary exposure all concentrations dem ...	1979	521052
the influence of environmental temperature on the rate of development of ascaris suum eggs in great britain.	the rate of development of ascaris suum eggs was recorded during 18 months in england. every second or third week unembryonated eggs were placed in an unheated animal house and their development was observed at weekly intervals. eggs underwent no development during november to may. embryonation was most rapid during june, july and august. the shortest time for eggs to reach the infective stage was two weeks in 1976 and four weeks in 1977. a relationship was apparent between the rate of developme ...	1979	523803
some properties of the sulphoxidases and sulphoxide reductases of the cestode moniezia expansa, the nematode ascaris suum and mouse liver.	1. the anthelmintics bithionol, phenothiazine, albendazole and fenbendazole were oxidized to sulphoxides by enzymes in the cytosol of the proglottids of the cestode moniezia expansa and the cytosol of the intestinal epithelial cells of the nematode ascaris suum. enzymes in these tissues were also able to reduce these sulphoxides to the thioethers in the absence of oxygen. 2. sulphoxidation and sulphoxide reduction also occurred in mouse liver enzyme preparations. about 20% of the sulphoxidation ...	1979	532217
induction, isolation and surface marker studies on bovine eosinophils.	a crude extract from ascaris suum was infused into the teat canal of heifers to serve as an irritant or an antigens. the cells present in the mammary gland following such stimulation were assessed over a period of 2 weeks. prior to stimulation there were few cells, predominantly macrophages, however, by 12 h post-stimulation a larger number of eosinophils and neutrophils were present. the eosinophils, which represented approximately 50% of the total population, could be purified by ficoll-hypaqu ...	1979	422852
depressed cyclic amp levels in airways smooth muscle from asthmatic dogs.	we tested mongrel dogs by intradermal injection and tracheobronchial aerosol challenge with ascaris suum antigen extract. all dogs were skin-test positive but could be segregated, on the basis of airways resistance measurements, into "asthmatic" (bronchoreactive) and "nonasthmatic" (nonbronchoreactive) groups. by using tracheal rings from these dogs, we measured the abilities of the beta-adrenergic agonist, isoproterenol, to relax tracheal smooth muscle contracted by methacholine and to cause cy ...	1979	220616
[effect of 0,0-diethyl-0-1-(2,4-dichlorphenyl)-2-bromovinylphosphate and 0,0-dimethyl-0-1-(2,4-dichlorphenyl)-2-bromovinylphosphate on the invasiveness of the ova of ascaris suum goeze, 1782].		1979	575005
antigen-induced bronchospasm in conscious sheep.	in 10 conscious ewes with cutaneous sensitivity to ascaris suum, measurements of pulmonary resistance (rl), static lung compliance (cst), functional residual capacity (frc), and arterial blood gas composition along with arterial plasma histamine concentration (h) were obtained before and after inhalation challenge with a. suum extract. five animals showed no changes in respiratory mechanics after a. suum challenge; in these h did not change. the remaining five animals responded to a. suum challe ...	1979	511716
experimental canine anaphylaxis: cyclic nucleotides, histamine, and lung function.	we studied the effects of ascaris suum antigen (iv) in 32 natively allergic, anesthetized dogs. after thoracotomy, bilateral cervical vagotomy, and propranolol, samples of peripheral lung frozen in situ were obtained for measurement of cyclic nucleotides and histamine. following ascaris, lung histamine decreased 20.4 +/- 3.7% (mean +/- se), camp increased 391 +/- 122%, and cgmp increased 110 +/- 20% with increased plasma histamine and physiological changes of anaphylaxis. no significant changes ...	1979	224010
serologic differentiation of adult toxocara and ascaris.	the antigenic analysis of toxocara canis a causative agent of visceral larva migrans and ascaris suum has been carried out. agar gel double diffusion techniques and its modifications were employed to determine whether or not crude antigens, obtained from the adult parasites: (a) contained components unique for each, (b) could be used to differentiate the parasites serologically. adult toxocara canis and ascanis suum antigens used in this study were found to contain several unique antigenic compo ...	1979	121695
[immunoelectrophoretic studies of several helminths]	in an attempt to investigate the antigen-antibody relations and the value of immunodiagnosis for several helminths, ouchterlony tests and immunoelectrophoreses were carried out. taenia saginata, cysticercus sp. of cestodes, clonorchis sinensis, fasciola hepatica and paramphistomum sp. of trematodes,and ascaris suum of nematodes were used as antigens. on the other hand, antisera were obtained by injecting 0.5 ml each of the above antigens and the same amount of complete freund's adjuvant into rab ...	1979	12902755
ultrastructural observations on the cell surface of the intestinal epithelium of the nematode, ascaris suum. nature of the electronegative charge.	the intestinal epithelium of ascaris suum consists of a single layer of tall columnar epithelial cells that rest on a thick basal membrane in contact with the pseudocoelomic cavity. experiments were conducted on glutaraldehyde-fixed tissue to ascertain the nature of the electronegative charges associated with both the apical microvillar surface and basal membrane. a strong electronegative charge was demonstrated on the microvillar surface and basal membrane with ruthenium red and cationic ferrit ...	1980	6153929
respiratory hypersensitivity to ascaris extract in guinea pigs sensitized by aerosol.	guinea pigs exposed via the respiratory route to ascaris suum extract in aerosol form became hypersensitive. the hypersensitivity was detectable by inhalational bronchial provocation testing using a noninvasive plethysmography, by chopped lung histamine release, and by passive cutaneous anaplylaxis. the antibody responsible for the hypersensitivity seems to be directly associated with pulmonary tissue. a locally produced cytotropic pulmonary antibody, probably igg or ige may be causal. histopath ...	1980	6159330
egg-shell permeability and hatching of ascaris suum.	when eggs of ascaris suum were transferred from glass-distilled water into 0.1--0.4 m nacl solutions, the water content of the unhatched juveniles fell with increasing concentration of solute. the effect was reversible. the egg-shell was thus permeable to water. the osmotic pressure of the egg fluid was osmotically equivalent to between 0.1 and 0.2 m trehalose. in hatching experiments in fairbairn's medium containing 0.1 or 0.2 m trehalose, only 5 and 3% respectively of the eggs hatched; 83% of ...	1980	7190259
prophylactic medication with pyrantel to prevent liver condemnation in pigs naturally exposed to ascaris infections.	the use of pyrantel tartrate (106 mg/kg of feed) as a continuous feed medication was evaluated in 848 finishing hogs for efficacy in preventing ascaris suum infections, and in reducing liver fibrosis and liver condemnation at slaughter, associated with a suum infections. liver condemnations due to ascaris damage were reduced 100% in all pigs given pyrantel, when compared with condemnation in non-medicated controls. none of the livers from any of the medicated animals was condemned, whereas liver ...	1980	7194010
sulfur dioxide induced airway hyperreactivity in allergic sheep.	the effects of a four-hour exposure (via a plexiglas hood) to sulfur dioxide (so2) on airway reactivity was studied in both normal and allergic conscious sheep. allergic sheep were defined as animals in whom inhalation of ascaris suum extract resulted in an increase in mean pulmonary flow resistance (rl). airway reactivity (delta rl) was assessed by measuring the increase in rl after 18 breaths of 0.25% carbachol, from an initial value obtained after 18 breaths of buffered saline. rl and delta r ...	1980	7342777
ascaris suum: nutrient absorption, growth, and intestinal pathology in young pigs experimentally infected with 15-day-old larvae.		1980	7350001
pharmacological characteristics of cholinoreception in somatic muscles of the nematode, ascaris suum.		1980	7364197
tubulin characterization during embryogenesis of ascaris suum.		1980	7380098
interaction of anthelmintic benzimidazoles with ascaris suum embryonic tubulin.	the ability of mebendazole and fenbendazole to bind to tubulin in cytosolic fractions from 8-day ascaris suum embryos was determined by inhibition studies with [3h]colchicine. colchicine binding in the presence of 1 . 10(-6) m mebendazole was completely inhibited during a 6 h incubation period at 37 degrees c. inhibition of colchicine binding to a. suum embryonic tubulin by mebendazole and fenbendazole appeared to be noncompetitive. the inhibition constants of mebendazole and fenbendazole for a. ...	1980	7388055
potentiation of the reaginic (ige) antibody response to ovalbumin in the guinea pig with a soluble metabolic product from ascaris suum.	a soluble antigen, the acf antigen, obtained from cultures of larvae of ascaris suum was able to potentiate the reaginic response to an unrelated antigen was administered at the same time or 4 days after ovalbumin, there was a weak primary response and a strong statistically significant secondary ige response, compared with animals immunized only with ovalbumin. this potentiated response was not observed when the acf antigen was presented 10 days before or 8 or 12 days after the ovalbumin.	1980	7391579
iodination of surface components of the spheroidal and ameboid spermatozoa of ascaris suum (nematoda).		1980	7397296
effect of local antigen inhalation and hypoxia on lobar blood flow in allergic dogs.	to determine the local pulmonary vascular response to inhaled antigen and alveolar hypoxia in canine asthma, 8 of 42 skin-test-positive dogs were selected from a preliminary study of airway reactivity to antigen challenge with an extract of ascaris suum. in an anesthetized, open-chest preparation subjected to left cervical vagotomy, the proportion of pulmonary blood flow to the left lower lobe (q lobe/q lung) was estimated by an insoluble gas-elimination method. continuous inhalation of antigen ...	1980	7406342
chromosome diminution in ascaris suum. chromatin structure.		1980	7408987
[action of cholinolytic substances on the somatic musculature of the nematode, ascaris suum].		1980	7421755
experimental ascaris suum infection in pigs. serological response, eosinophilia in peripheral blood, occurrence of white spots in the liver and worm recovery from the intestine.	experimental oral inoculation of pigs with infective a. suum eggs resulted in eosinophilia in the peripheral blood and a serum antibody response. when pigs were inoculated at 3 days of age the antibody response was depressed which is suggested to be due either to a protective effect of colostral antibodies or to an immunologic incompetence of the young pigs. despite the use of isolated pens and strict hygienic measures it was difficult to obtain an ascaris free environment for the experimental a ...	1980	7433010
basenji-greyhound dog model of asthma: reactivity to ascaris suum, citric acid, and methacholine.	bronchial inhalation challenges to ascaris suum, citric acid, and methacholine were performed in eight basenji-greyhound (bg) crossbreed dogs, five of which were reactive to ascaris antigen aerosols (aaa). responses to aerosol challenges were measured as changes in pulmonary resistance (rl) and dynamic compliance (cdyn) and were compared to responses obtained in five mongrel dogs. responses to methacholine and citric acid were similar in bg dogs whether ascaris-sensitive or not, but bg dogs were ...	1980	7440303
homology between roundworm (ascaris) and hookworm (n. americanus) antigens detected by human ige antibodies.	utilizing the techniques of radioallergosorbent test (rast), inhibition of rast and isoelectric focusing on polyacrylamide gels, antigenic cross-reactivity has been shown between extracts of ascaris lumbricoides. ascaris suum and necator americanus (hookworm). antigenic homology is extensive between the two ascaris preparations. the antigens common to hookworm and ascaris, while representing a minor component of ascaris body fluid, are nonetheless potent stimulators of ige antibody production in ...	1980	7458774
ascaris suum in saskatchewan pigs: an abattoir survey of prevalence and intensity of infection.	in a survey of 2500 market weight pigs in a saskatchewan abattoir, 37% were infected with adult ascaris suum and in 46% there were milkspot hepatic lesions. a total of 60% of the pigs examined had some evidence of a. suum infection. most infected pigs contained less than 50 adult ascarids. in a second abattoir survey of a further 2500 market weight pigs, 44% of the animals had milk-spot hepatic lesions. in approximately 12% of the affected pigs these lesions were very severe and a very similar p ...	1980	7459794
[allergen-induced airway obstruction and histamine concentration in arterial and venous plasma. first communication (author's transl)].	in 19 dogs sensitized against ascaris suum extract we measured dynamic elastance, arterial plasma histamine concentration, pressure in the a. femoralis, and arterial blood gases. in six dogs the venous plasma histamine concentration was also analyzed. ascaris extract (a.e.) inhalation increased both the arterial and the venous plasma histamine concentration significantly. the venous concentration was 61% of that detected in the arterial blood. at the same arterial plasma histamine level a large ...	1980	7444174
[the place of attack of beta-2 receptor stimulating bronchodilators. fenoterol (berotec) in allergic airway obstruction in dogs, hypersensitive to ascaris suum].	on dogs sensitive against ascaris suum extract an increase of the airway resistance can be caused by challenge alone on the upper part or the lower part of the airways by ascaris suum extract. fenoterol as beta 2-receptor stimulator is capable to decrease this reaction by deposition of this drug on the upper part as on the lower part of the airways. the effect of fenoterol on the upper part of the airways is much stronger and lasts much longer than on the lower part of the airways or by intraven ...	1980	6248681
intestinal basement membrane of ascaris suum. properties of the collagenous domain.		1980	6768727
[the dynamics of important helminths in pig husbandry].	the growth and seasonal dynamics of three helminth species (ascaris suum, oesophagostomum dentatum, and trichocephalus suis) were studied on a modern pig-fattening farm of the pm-016-agp type. the study of the dynamics of the incidence of the eggs of these helminth species demonstrated that large swine roundworm (ascaris suum) had favourable conditions for further development at average daily temperatures above 12 degrees c. in winter when the average daily temperatures were lower than 10 degree ...	1980	6769208
[the effect of technological and zoo-hygienic factors on the occurrence of helminths in different strains of pigs].	seven helminth species were found in 14 different pig stocks in the south bohemian region. these were: ascaris suum, oesophagostomum dentatum, trichocephalus suis, hyostrongylus rubidus, strongyloides ransomi, capillaria sp. and metastrongylus sp. o. dentatum dominates in adult animals. a. suum, t. suis and s. ransomi are more frequently encountered in piglets and gilts. the primiparous sows can be therefore regarded as the main source of invasion for piglets. the composition of the animals' hel ...	1980	6773221
a survey of gastrointestinal parasitism in pigs.	over a period of two years 180 pork pigs, 144 baconers and 144 sows from two slaughterhouses in northern england were examined for gastrointestinal parasites. sows showed the highest level and intensity of infection, 85 per cent harbouring the dominant parasites oesophagostomum dentatum and o quadrispinulatum; the highest worm burden recorded was 21,000. hyostrongylus rubidus was present in 28.5 per cent of sows but virtually absent from younger pigs. small numbers of ascaris suum and trichuris ...	1980	7257108
a case of ascaris suum infection in lambs.		1980	7257116
ascaris oocytes: ultrastructural and immunocytochemical changes during passage through the oviduct.	slender, bipolar, immature, ascaris suum oocytes assume a smooth, spherical configuration as they traverse the oviduct. the present investigation was conducted to determine if this morphological alteration might be accompanied by changes in surface membranes. immunocytochemical studies were conducted utilizing (1) fluorescein-labeled, sheep, anti-rabbit igg, (2) sternberger's unlabeled, antibody-enzyme technique, (3) ferritin-labeled, sheep, anti-rabbit igg, and (4) an immunobead, labeled-antibo ...	1980	6993646
comparison of the responsiveness to histamine and to ascaris suum challenge in dogs.	the pulmonary and dermal sensitivity of a group of mongrel dogs to an extract of ascaris suum protein was compared with the pulmonary and dermal sensitivity to histamine in this same group. we found a modest but significant correlation in the entire population between skin test reaction to histamine and to a. suum protein (r = 0.52, p < 0.05), but when the skin test results were compared with those of aerosol challenge, no significant correlation was found. however, when those dogs with reaction ...	1980	7447158
the involvement of sugars and mg++ in the attachment of phagocytic cells to ascaris suum juveniles.	in vitro adherence of washed peritoneal exudate cells (pecs) from mice to ascaris suum juveniles was inhibited by d-glucosamine and d-galactosamine. other carbohydrates tested had no apparent effect on adherence after incubation for 3 hr at any of the concentrations tested. furthermore, attachment of pecs to a. suum juveniles was completely inhibited by the addition of ethylenediaminetetraacetate to the medium, but magnesium ethylene glycol bis(trichloroacetate) had no effect on adherence. the r ...	1981	7217714
ascaris suum: t-cell responses of c57bl/6j mice in vitro and in vivo.		1981	6970679
electrophoretic analyses of myofibrillar proteins from the body wall muscles of ascaris suum.	a myofibrillar protein extract has been isolated from the muscle of ascaris suum. two-dimensional electrophoresis of this extract revealed that the myosin light chain 1 (alc1) migrates as 3 components with approximate isoelectric points in the range of 5.3-5.6. the most acidic component of alc1 appeared to be phosphorylated when the myofibrillar extract was incubated for 10 s with catalytic subunit of camp dependent protein kinase and [gamma-32p] atp. the myosin light chain 2 (alc2) migrated as ...	1981	6894780
indirect solid-phase microradioimmunoassay for detection of ascaris suum antibodies in swine sera.	antibodies to ascaris suum have been detected and quantitated in sera of swine orally inoculated with eggs of a suum, using an indirect solid-phase microradioimmunoassay. antibody titer of 1:640 to 1:10,240 were quantitated, using purified and crude ascarid antigens in the assays. swine sera from 71 of 72 farm herds located in 37 counties in nebraska had at least 1 serum of the 7 tested with a titer equal to or greater than 1:20. a total of 437 sera were positive and 67 negative (ie, less than 1 ...	1981	7258808
efficacy of fenbendazole against five genera of swine parasites.	thirty-three pigs were sequentially infected with oesophagostomum spp, metastrongylus spp, and trichuris suis while being maintained in lots contaminated with space ascaris suum, strongyloides ransomi, and other parasites. pigs were assigned to 1 of 3 groups on the basis of weight (6.8 to 25.9 kg), sex, and litter. pigs in the 1st group were used as nontreated controls; pigs in the 2nd and 3rd groups were given fenbendazole in the feed at doses of 3 and 5 mg/kg of body weight, respectively, on 3 ...	1981	7271035
pathogenesis of liver lesions in mice following a primary infection with ascaris suum.	the development of liver lesions in mice infected with ascaris suum is described and compared to the lesions in pigs. the findings suggest that liver necroses in mice are initially periacinar (centrilobular), caused by anoxia resulting from a vascular obstruction by the a. suum larvae located in the sinusoids; and that the lesions in pigs following a primary infection have a different pathogenesis. it is concluded that mice are unsuitable models for studying the pathogenesis of a. suum-induced l ...	1981	7292896
immunopathology of ascarid infection of the eye. role of ige antibodies and mast cells.	the roles of ige antiascarid antibodies and mast cells were compared in passively sensitized guinea pigs and animals infected intravitreally with ascarid larvae (toxocara canis, ascaris suum). intravenous ige antibody disappeared from the serum within 48 hours, but induced a hypersensitive state that persisted for 28 days. in systemically immunized animals, the aqueous-serum ige antibody ratio was 1:1,000 or less. passive periocular anaphylactic reactions produced an infiltration of neutrophils ...	1981	7295137
accessory nuclei in female ascaris suum.	accessory nuclei were observed in early meiotic prophase (leptotene) in female ascaris suum but such structures were absent in meiosis in males. accessory nuclei also were observed during the prediminution embryonic divisions. all embryos did not have accessory nuclei, thus, those embryos with these structures may be female since the accessory nuclei are maternal-limited. accessory nuclei were not observed after the fourth embryonic division, at which stage the process of chromatin diminution be ...	1981	7299580
[the analysis of immunologically similar substance with ascaris suum specific protein in rat colon mucosa (author's transl)].		1981	7321258
[effect of glycerin, paraffin, rapeseed oil and aqueous solutions on the development of ova of ascaris suum goeze, 1782].		1981	7324462
in vitro development of ascaris suum from third- to fourth-stage larvae and detection of metabolic antigens in multi-well culture systems.	a population of early to late third-stage larvae (l3) of ascaris suum obtained from the lungs of swine 7 days after infection developed to the fourth stage (l4) in stationary, multi-well, culture plates in an atmosphere of 5% co2 in air. larval survival, growth and morphogenesis were evaluated in five culture systems consisting of dulbecco's modified eagles' medium alone (dm) or containing a serum supplement (dm-s) or a tripeptide (1-glycyl-1-histidyl-1-lysine) at final concentrations of 20, 200 ...	1981	7328453
coupling of mitochondrial nadph : nad transhydrogenase with electron transport in adult hymenolepis diminuta.	the mitochondrial electron transport system of adult hymenolepis diminuta exhibited an apparent specificity in terms of reduced pyridine nucleotide utilization. the preferred substrate for both the minor oxidase and the physiologically required fumarate reductase system was nadh. intramitochondrial reducing equivalents, needed for phosphorylation via the anaerobic, electron transport-dependent, fumarate reductase, were generated as nadph by the action of the cestode's nadp-specific "malic" enzym ...	1981	7328455
intestinal tubulin as possible target for the chemotherapeutic action of mebendazole in parasitic nematodes.	in vitro incubation of the parasitic nematode ascaris suum in the presence of 10 microm mebendazole (mbz) resulted in a complete loss of colchicine binding ability of extracts obtained from the parasite's intestine. biochemical evidence supported the identification of the colchicine binding receptor in a. suum intestinal extracts as tubulin. this protein was partially purified and found to comprise approximately 0.8% of the soluble intestinal protein. mbz inhibited colchicine binding to the part ...	1981	7335116
efficacy of ivermectin against five genera of swine nematodes and the hog louse, haematopinus suis.	twenty pigs sequentially infected with oesophagostomum spp, metastrongylus spp, and trichuris suis, and maintained on lots contaminated with ascaris suum and strongyloides ransomi, were assigned to 1 of 4 groups on the basis of weight, sex, and litter. each group of pigs was given ivermectin subcutaneously at the rate of 0, 20, 100, or 500 microgram/kg of body weight. lice on pigs were counted before treatment, on posttreatment day 1, and just before necropsy. pigs were necropsied on posttreatme ...	1981	6895286
diagnosis of human strongyloidiasis by immunofluorescence, using strongyloides ratti and s. stercoralis larvae.	the sensitivity and specificity of an indirect immunofluorescent antibody assay for the diagnosis of human strongyloidiasis has been investigated. sera were obtained from 160 australian ex-servicemen who had been prisoners-of-war in southeast asia during world war ii, 44 of whom proven parasitologically to have strongyloidiasis; these men did not have concurrent infections with other helminths. in addition, sera were collected from 44 age- and sex-matched australians who were not thought to have ...	1981	7015889
[serological differentiation of ascaris suum and toxocara canis infections in pigs (author's transl)].	groups of eight miniature pigs were infected either with 1,500 embryonated eggs each of ascaris suum or toxocara canis or with 1,000 eggs each of both nematodes. sera were sampled before the infection as well as three and six weeks postinfection and then investigated in the elisa on microtitre plates against antigens of various developmental stages. when extracts of adult ascaris and toxocara worms were used as antigens, distinct reactions were registered both in the infected and in the uninfect ...	1981	7032103
serodiagnosis of human strongyloidiasis by an enzyme-linked immunosorbent assay.	the sensitivity specificity of an enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of strongyloidiasis has been investigated. 45 men with long-standing strongyloidiasis were compared with the same number of age- and sex-matched control subjects. the elisa detected antibody in 84% of patients with parasitologically proven strongyloidiasis. when the technique was compared with an indirect immunofluorescent assay (ifa), a high correlation coefficient was obtained. specificity was dem ...	1981	7036430
[serological demonstration of experimental round worm infections-ascaris suum, toxocara canis--in swine by means of the indirect immunofluorescence antibody test].	by means of indirect immunofluorescent antibody reaction (ifar), using serum of experimentally infected pigs, various antigens were studied with regard to their usefulness for serological verification of prepatent ascaris suum and toxocara canis infections. eggs, egg larvae, larvae received from livers, lungs and brains of experimentally infected white mice and sections of adult t. canis as well as eggs, egg larvae, liver larvae and sections of frozen adult a. suum proved to be not suitable for ...	1981	7039425
intestinal basement membrane of ascaris suum. physical properties of the collagenous domain.		1981	6783646
activity of enzymes regulating glycogen metabolism in perfused muscle-cuticle sections of ascaris suum (nematoda).	a new perfusion system has been developed in which muscle-cuticle sections of ascaris suum were perfused, enabling study of enzymes in vitro. using this technique the activity of the regulatory enzymes glycogen synthase and glycogen phosphorylase was determined, and the level of glycogen in the muscle was assessed. during starvation, 98% of glycogen synthase was in the inactive d-form, and 80% of the glycogen phosphorylase activity was in the active a-form. when the ascarid muscle section was pe ...	1981	6790691
glycogen metabolizing enzymes during starvation and feeding of ascaris suum maintained in a perfusion chamber.	the glycogen content of muscle was correlated with the activity of glycogen synthase and glycogen phosphorylase from the parasitic roundworm ascaris suum maintained in vitro. adult female worms were maintained in the laboratory in a perfusion system during periods of starvation and feeding. during starvation, the levels of glucogen decreased at a rate of 0.1 to 0.2 mumoles/min/g wet weight of muscle-cuticle. during this time, 95% of the glycogen synthase (e.c. 2.4.1.11) was in the active d-form, ...	1981	6790695
effect of pharmacologic agents on antigen-induced decreases in specific lung conductance in sheep.	the purpose of this investigation was to determine the primary mediators responsible for the decrease in specific lung conductance (sgl) after airway challenge with ascaris suum antigen in allergic sheep. on different occasions, separated by 10 to 14 days, pulmonary resistance and thoracic gas volume were measured in 5 sheep with ascaris suum hypersensitivity before and for 2 h after a standard inhalation challenge with this antigen. initially and at the end of the study, inhalation challenge de ...	1981	6795979
surface receptors: are they involved in transformation of spermatozoa of ascaris?	exposure of the spheroidal spermatozoa of ascaris suum to an extract of the male accessory gland causes their transformation into ameboid cells. we have investigated the mechanism of this transformation, also termed activation, by labeling the proteins of accessory gland extracts with fluorescein isothiocyanate (fitc) or [125i], followed by qualitative localization of the sperm activating substances (sas) and quantitative measurements of [125i]-sas binding. fluorescent patches of fitc-conjugated ...	1981	6260362
[survival of ascaris eggs, salmonella and fecal coli in soil and on vegetables grown in infected soil (author's transl)].	in an experiment it was shown that eggs of ascaris suum, salmonella and fecal coliform bacteria mixed with sewage sludge and added to soil, will survive the first growing season. most eggs of ascaris suum in soil embryonated within a period of 7--15 weeks. in soil without a protective vegetation the concentration of eggs in the top soil layer 0--3 cm decreased faster than in soil with a protective vegetation. at the same time there seemed to be a corresponding increase in the concentration of eg ...	1981	6261218
restriction enzyme analysis of the germ line limited dna of ascaris suum.	the germ line limited dna of ascaris suum was isolated from sperm and testis as a satellite dna component in hoechst 33 258 -- cscl gradients. employing restriction enzyme analysis, we show that the germ line limited dna is composed entirely of two families of tandemly repeated sequences, one repeat unit is 125 bp, and the other 131 bp long. the total appr. 5 x 10(5) copies of the two families are physically separated from each other (segmental arrangement). several repeat unit variants within b ...	1981	6268372
serotonin (5-hydroxytryptamine): a possible regulator of glycogenolysis in perfused muscle segments of ascaris suum.		1981	6269541
differences in airway reactivity in normal and allergic sheep after exposure to sulfur dioxide.	the effect of breathing 5 ppm sulfur dioxide (so2) on airway reactivity was studied in both normal and allergic conscious sheep. allergic sheep were defined as animals in which inhalation of ascaris suum extract resulted in bronchospasm as evidenced by an increase in mean pulmonary flow resistance (rl), hyperinflation, and a fall in dynamic compliance. airway reactivity was assessed by measuring the increase of rl after 18 breaths of 0.25% carbachol (c), from an initial rl value obtained after 1 ...	1981	7319895
subcellular fractions and the refringent granules of the spermatozoa of ascaris suum (nematoda).	six subcellular fractions were isolated by differential centrifugation of the homogenate of spermatozoa of ascaris suum. the cellular constituents of pelleted fractions, as identified by electron microscopy, were membranes and membranous organelles (fraction a1), microsomal (a2), cytoplasmic (a3), large refringent granules (b1), small refringent granules (b2) and a detergent-soluble fraction (b3). polypeptide analysis of sds-page showed that the 18,400-dalton band, one of the major spermatozoan ...	1981	7317939
suppression of reaginic antibodies with modified allergens. iii. preparation of tolerogenic conjugates of common allergens with monomethoxypolyethylene glycols of different molecular weights by the mixed anhydride method.	our previous findings that antigens, such as ovalbumin (oa) and the extract of ragweed pollen (rag), could be rendered nonantigenic, nonallergenic and tolerogenic by conjugation with polyethylene glycol (peg) have been extended in the present study to the synthesis of conjugates of a variety of antigens with monofunctional monomethoxy-pegs (mpegs) of different molecular weights by the use of the mixed anhydride method. thus, mpegs with molecular weights of 2,000, 5,000, 10,000 and 20,000 were co ...	1981	7450906
effects of atmospheric ammonia on young pigs experimentally infected with ascaris suum.	effects of atmospheric ammonia at 69.4 mg/m3 (100 ppm) on productive performance and respiratory tract health of young pigs (starting body weight averaged 7.5 kg) experimentally infected with ascaris suum (50,000 embryonated ova administered by gavage when pigs were 5 weeks of age) were studied in 5 trials of 4 weeks each (when pigs were 5 to 9 weeks of age). effects of atmospheric-ammonia exposure and ascarid infection on growth were additive. compared with controls, percentage reductions in av ...	1981	7283247
the basenji-greyhound dog: antigen-induced changes in lung volumes.	subdivisions of lung volume, closing volume (cv), pulmonary resistance (rl) and dynamic pulmonary compliance (cdyn) were measured before and after bronchial challenge with ascaris suum in seven ascaris-sensitive basenji-greyhound (bg) crossbreed dogs. rl increased from a control value of 1.41 +/- 0.43 (mean +/- se) cm h2o/l/sec to 21.4 +/- 5.15 (p less than 0.01) and cdyn decreased from 163 +/- 32 ml/cm h2o to 43 +/- 11 (p less than 0.01) in the first 15 min following ascaris-antigen aerosol (aa ...	1981	7280384
impaired tracheal mucus transport in allergic bronchoconstriction: effect of terbutaline pretreatment.	the transport velocity of mucus within the trachea, pulmonary resistance, and arterial blood gas composition were measured in intubated conscious sheep with ascaris suum sensitivity before and during allergic bronchoconstriction. inhalation of a. suum extract for 15 min increased mean pulmonary resistance significantly from 1.9 cm h2o . l-1 sec-1 to 5.1 cm h2o . l-1 sec-1 after 60 min and to 4.5 cm h2o . l-1 sec-1 after 120 min, while it decreased mean arterial po2 from 85 to 59 and 53 mm hg, re ...	1981	7229224
anthelmintic activity of oxfendazole in pigs.	eighty-five pigs were artificially infected with hyostrongylus rubidus, oesophagostomum species and ascaris suum. on days 2, 10, 20 or 51 after infection groups of six were treated with oxfendazole premix in food at various dose rates. subsequently the pigs were slaughtered for comparative worm counts to be made in treated and control animals. the efficacy of treatment against hyostrongylus worms of increasing age was 68, 57, 99.8 and 100 per cent after a 3 mg per kg dose, 75, 75, 99.8 and 100 p ...	1981	7233774
purification of malic enzyme from ascaris suum using nad+-agarose.	malic enzyme has been purified from ascaris suum by polyethylene glycol precipitation, ion-exchange chromatography, ammonium sulfate precipitation, and nad-agarose affinity chromatography to a specific activity of 80 units/mg (v/[e]t = 350 s-1). the preparation was shown to be homogeneous by sds polyacrylamide disc gel electrophoresis. the procedure can be accomplished in a maximum of four days with a 74% yield.	1981	7242572
bone marrow cells associated with heightened eosinophilopoiesis: an electron microscope study of murine bone marrow stimulated by ascaris suum.	an acute eosinophilopoiesis occurs in mice secondarily exposed to ascaris suum, the marrow eosinophils increasing from approximately 5% to 45%, and blood levels from 50/ml3 to more than 1000/ml3, within 14 days. eosinophilopoiesis occurs in hematopoietic compartments of the bone marrow and is associated with four other cell types: branched stromal cells, macrophages, lymphocytes, and reticular cells adventitial to venous sinuses. branched stromal cells, a newly recognized cell type, are presente ...	1981	7246451
eosinophilia in athymic nude (rnu/rnu) rats--thymus-independent eosinophilia?	we have investigated the ability of genetically athymic (nude) rats to develop an eosinophilia to stimuli which are known to cause an eosinophilia in normal rats. nude rats developed eosinophilia following (a) the pulmonary embolization of bovine gammaglobulin (bcg)-coated latex particles, and (b) experimental infection with the nematode parasite ascaris suum. eosinophilia in nude rats was not dependent on the presence of specific homocytotropic antibodies, haemagglutinating antibodies or allerg ...	1981	6166546
effect of intravenous histamine, allergen (ascaris suum extract) and compound 48/80 and inhaled allergen-aerosol on bronchoconstriction and histamine release.	the effect of a histamine releaser (48/80), an antigen (ascaris suum extract) and of exogenous histamine infusion on antigen aerosol-induced bronchoconstriction was investigated in 24 mongrel dogs. during these substance infusions, the high values of arterial plasma histamine did not correlate with the degree of bronchoconstriction. further, a severe bronchoconstriction with a relatively low arterial plasma histamine concentration was observed during antigen inhalation. during substance inhalati ...	1981	6173907
fenbendazole as a therapy for naturally acquired stephanurus dentatus and gastrointestinal nematodes in feral swine.	adult feral swine, naturally infected with kidney worms (stephanurus dentatus) and gastrointestinal nematodes, were divided into two groups of 10 pigs each. one group was treated with fenbendazole (panacur, hoechst ag, frankfurt am. main) mixed in feed at the rate of 3 mg kg-1 body weight for 3 days. the second group received feed only and was designated as non-treated controls. the animals in both groups were necropsied 3 weeks post-treatment and examined for the presence of live and dead adult ...	1981	6211818
[an epidemiological study on zoonoses in korea]	helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ...	1981	12902720
[a comparative analysis of various parts of ascaris suum with respect to their protein composition]	for the purpose of making a comparative study of protein compositions in ascaris suum by sexes and body parts, extracts were prepared from whole bodies, body walls, genital organs, digestive organs and body fluid, of both sexes. and electrophoretic analysis was conducted using polyacrylamide slab gel in the presence of sodium dodecyl sulfate. the results were as follows: 1. in this study, protein bands of each part were separated in the largest number and most clearly under 8%~12 % (10 %) gradie ...	1981	12902713
mitochondrial ultrastructural and atpase changes during the life cycle of ascaris suum.		1982	6216389