Publications

[riboflavin overproduction in 4-aminopyrazole[3,4-d]pyrimidine-treated yeast pichia guilliermondii].	more than 90 mutants resistant to the adenine analogue 4-aminopyrazole[3,4-d]pyrimidine (4-app), were isolated from a wild-type strain of yeast pichia guilliermondii. some of appr mutants accumulated noticeable amounts of products absorbing at 260 nm in the culture medium, probably nucleotides and their derivatives. in comparison to the parent strain, the mutant appr-27 synthesized greater amounts of xanthine and uracil suggesting the presence of defects in the regulation of de novo biosynthesis ...	2002	12068578
cutaneous candidiasis in a dog caused by candida guilliermondii.		2002	12081309
new chromogenic agar medium for the identification of candida spp.	a new chromogenic agar medium (candida diagnostic agar [cda]) for differentiation of candida spp. is described. this medium is based on sabouraud dextrose agar (oxoid cm41) and contains (per liter) 40.0 g of glucose, 10.0 g of mycological peptone, and 15.0 g of agar along with a novel chromogenic glucosaminidase substrate, ammonium 4-(2-[4-(2-acetamido-2-deoxy-beta-d-glucopyranosyloxy)-3-methoxyphenyl]-vinyl)-1-(propan-3-yl-oate)-quinolium bromide (0.32 g liter(-1)). the glucosaminidase substrat ...	2002	12089051
influence of ph on the xylose reductase activity of candida guilliermondii during fed-batch xylitol bioproduction.	the influence of ph on the xylose reductase (xr) activity was studied. the fed-batch fermentation using exponential feeding rate was employed to produce xylitol. the feeding started when the xylose concentration in the fermenter reached 50 g/l and the ph was adjusted to 2.5, 4.0 or 6.0. the best results for xr activity (0.567 u/mg(protein)) and xylitol volumetric productivity (1.06 g/l.h) were achieved with ph 6.0.	2002	12111747
metabolic behavior of immobilized candida guilliermondii cells during batch xylitol production from sugarcane bagasse acid hydrolyzate.	candida guilliermondii cells, immobilized in ca-alginate beads, were used for batch xylitol production from concentrated sugarcane bagasse hydrolyzate. maximum xylitol concentration (20.6 g/l), volumetric productivity (0.43 g/l. h), and yield (0.47 g/g) obtained after 48 h of fermentation were higher than similar immobilized-cell systems but lower than free-cell cultivation systems. substrates, products, and biomass concentrations were used in material balances to study the ways in which the dif ...	2002	12115432
[the photodynamic inactivation of candida guilliermondii in the presence of photodithazine].	photodithazine, a glucosamine salt of chlorin e6, enhanced the inactivation of candida guilliermondii cells by visible light. the sensitizing effect of photodithazine was found to be related to free or cell surface-bound molecules of this dye. sodium azide (a singlet oxygen quencher) and propyl gallate (an inhibitor of lipid peroxidation) protected yeast cells from the photodithazine-enhanced photoinactivation.	2002	12138756
[the reversion of pichia guilliermondii transformants to the wild-type phenotype].	pichia guilliermondii strain with blocked gtp cyclohydrolase ii was transformed using replicative plasmids and their fragments containing the structural gene rib7 of this enzyme. experiments showed that the presence of an ars element and the promoter region of the gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. different types of recombination in the yeast p. guilliermondii are discussed.	2002	12138759
esterase activity in various candida species.	the aim of the present study is to ascertain esterase activities of various species of candida. a total of 125 strains isolated and identified by conventional methods were tested for esterase activity using the tween 80 opacity test. our results showed that 58 of 59 strains of candida albicans, all of the candida tropicalis strains (n = 38) and all of the candida guilliermondii strains (n = 3) produced positive results, whereas the remaining candida species did not. the tween 80 opacity test is ...	2002	12166351
pathology of a mouse model of x-linked chronic granulomatous disease.	a colony of knockout mice (gene designation cybb tm1) has been maintained at this institution for 5 years. these mice are lacking the b subunit of nadph oxidase and are susceptible to experimental infection with aspergillus fumigatus. the purpose of this study was to document the spontaneous diseases present in these mice which are a murine model of x-linked chronic granulomatous disease and to compare these lesions to those of chronic granulomatous disease in humans. lesions were documented in ...	2002	12213046
fermentability of grape must after inhibition with dimethyl dicarbonate (dmdc).	dimethyl dicarbonate (dmdc) was added to grape must and to synthetic media and results showed that, at 20 degrees c, 150 mg.l(-)(1) dmdc completely inhibited the fermentation of a grape must that was previously inoculated with 10(6) cells.ml(-)(1) saccharomyces bayanus and saccharomyces uvarum. brettanomyces intermedius, candida guilliermondii, hansenula jadinii, hansenula petersonii, kloeckera apiculata, pichia membranaefaciens, and saccharomyces cerevisiae were inhibited by 250 mg.l(-)(1). can ...	2002	12236685
comparison of the antifungal susceptibility testing subcommittee of the european committee on antibiotic susceptibility testing proposed standard and the e-test with the nccls broth microdilution method for voriconazole and caspofungin susceptibility testing of yeast species.	the proposed standard of the antifungal susceptibility testing subcommittee of the european committee on antibiotic susceptibility testing (afst-eucast) and the e-test procedures were compared with the nccls reference broth microdilution method for voriconazole and caspofungin susceptibility testing of 102 clinical candida species and saccharomyces cerevisiae isolates. the voriconazole mic at which 50% of strains were inhibited (mic(50)) was < or =0.125 mg/liter for all yeast species except for ...	2002	12354895
[cloning and expression of a gene encoding riboflavin synthase of the yeast pichia guilliermondii].		2002	12379014
native valve endocarditis due to pichia ohmeri.	candida species can cause clinical manifestations in various organs of the cardiovascular system, i.e., the pericardium, myocardium, and endocardium, with endocarditis being the best-known clinical entity. endocarditis is seen primarily in intravenous drug users and in individuals with damaged native valves, especially in congenital heart disease or rheumatic valvular diseases, and in prosthetic heart valves. the authors present a case of pichia ohmeri endocarditis in an intravenous drug user, w ...	2002	12382036
in vivo pathogenicity of eight medically relevant candida species in an animal model.	the relative pathogenicity of eight medically important candida species was investigated in a mouse model.	2002	12382088
identification and antifungal susceptibility of candida isolated from intensive care unit patients.	candida was isolated in 205 of 1060 clinical specimens (19.33%) in our laboratary sent from the intensive care unit for mycological investigation between january 98-december 99. all isolated strains were identified to species level using the api candida system (bio-meieux, france) as follows; candida albicans (n:115, 56.09%), candida tropicalis (n:23, 11.21%), candida parapsilosis (n:21, 10.24%), candida glabrata (n:12, 5.83%). candida kefyr (n:9, 4.39%), candida lusitaniae (n:7, 3.41%), candida ...	2002	12437230
biodegradation of vocs from printing press air by an on-site pilot plant bioscrubber and laboratory scale continuous yeast cultures.	the volatile organic compound composition (vocs) of printing press air was found to contain mostly ethanol, but also ethyl acetate, 1-propanol, 2-propanol, 1-methoxy-2-propanol and 3-ethoxy-1-propanol. a pilot plant bioscrubber inoculated with a mixed microbial population was constructed on-site. the bioscrubber was able to treat the polluted gas efficiently. it, however, suffered from strong wall growth and blockages in the column. the efficiencies of the pilot plant and a bioreactor is compare ...	2002	12449318
fungal infection of the radicular dentin.	although fungi have been detected in infected root canals, their precise role as endodontic pathogens has not yet been elucidated. the purpose of this study was to investigate the pattern of radicular dentin colonization by five fungal species. bovine root sections were infected with each of the following fungal species: candida albicans, candida glabrata, candida guilliermondii, candida parapsilosis, and saccharomyces cerevisiae. after 14 days, the sections were fixed in glutaraldehyde, split i ...	2002	12470022
improvement in xylitol production from sugarcane bagasse hydrolysate achieved by the use of a repeated-batch immobilized cell system.	candida guilliermondii cells were immobilized in ca-alginate beads and used for xylitol production from concentrated sugarcane bagasse hydrolysate during five successive fermentation batches, each lasting 48 hours. the bioconversion efficiency of 53.2%, the productivity of 0.50 g/l x h and the final xylitol concentration of 23.8 g/l obtained in the first batch increased to 61.5%, 0.59 g/l x h and 28.4 g/l, respectively, in the other four batches (mean values), with variation coefficients of up t ...	2002	11930897
controlled transient changes reveal differences in metabolite production in two candida yeasts.	physiological responses during growth on xylose and the xylose-degrading pathway of candida tropicalis and candida guilliermondii yeasts were investigated. the responses to a linearly decreasing oxygen transfer rate and a simultaneously increasing dilution rate were compared. c. guilliermondii produced acetate but no ethanol, and c. tropicalis ethanol but no acetate under oxygen limitation. both strains produced glycerol. the d-xylose reductase of c. guilliermondii is exclusively nadph-dependent ...	2002	11954799
effect of competition and adverse culture conditions on aflatoxin production by aspergillus flavus through successive generations.	strains of aspergillus flavus often degenerate with serial transfers on culture media, resulting in morphological changes and loss of aflatoxin production. however, degeneration does not readily occur in nature as indicated by the wild-type morphological characters of newly isolated strains and the high percentage of aflatoxigenic a. flavus from soil and crops in some geographic regions. in this study, three aflatoxin-producing strains of a. flavus were serially transferred using conidia for 20 ...	2002	21156547
high-dose liposomal amphotericin b in the therapy of systemic candidiasis in neonates.	high-dose (5-7 mg/kg/day) liposomal amphotericin b was evaluated prospectively during the period 1995-2001 in 41 episodes of systemic candidiasis occurring in 37 neonates (36 of the 37 were premature infants with very low birth weights). median age at the onset of systemic candidiasis was 17 days. candida spp. were isolated from blood in all patients and from urine, skin abscesses and peritoneal fluid in 6, 5 and 1 neonates, respectively. candidiasis was due to candida parapsilosis in 17 cases, ...	2003	13680398
bioremediation of chromium by the yeast pichia guilliermondii: toxicity and accumulation of cr (iii) and cr (vi) and the influence of riboflavin on cr tolerance.	a comparative study has been made on the sensitivity of the yeast pichia guilliermondii to cr (iii) and cr (vi) as well as on the cr uptake potential at growth-inhibitory concentrations of chromium. the strains used in the study were either isolated from natural sources or obtained from a laboratory strain collection. the results show that most of the natural strains were more tolerant to chromium and were able to grow in the presence of 5 mm cr (iii) or 0.5 mm cr (vi), that is at concentrations ...	2003	12608581
bioconversions of maize residues to value-added coproducts using yeast-like fungi.	agricultural residues are abundant potential feedstocks for bioconversions to industrial fuels and chemicals. every bushel of maize (approximately 25 kg) processed for sweeteners, oil, or ethanol generates nearly 7 kg of protein- and fiber-rich residues. currently these materials are sold for very low returns as animal feed ingredients. yeast-like fungi are promising biocatalysts for conversions of agricultural residues. although corn fiber (pericarp) arabinoxylan is resistant to digestion by co ...	2003	12702445
occurrence of yeasts in municipal wastes and their behaviour in presence of cadmium, copper and zinc.	seven yeasts strains have been isolated from sewage sludge. also six samples of compost with different sieving, composting times and origins, have been analysed. apparently, composting processes negatively affect the viability of yeasts, as none could be isolated from the compost samples. the margins of tolerance of the yeasts to cd, cu and zn have been determined. the physiological response to metals was similar in all the species studied, and in general, kinetic parameters (mu and lag) were af ...	2003	12761769
xylitol production from high xylose concentration: evaluation of the fermentation in bioreactor under different stirring rates.	to investigate the production of xylitol by the yeast candida guilliermondii fti 20037, in a bioreactor, from rice straw hemicellulosic hydrolysate with a high xylose concentration.	2003	12859766
xylitol production from sugarcane bagasse hydrolyzate in fluidized bed reactor. effect of air flowrate.	cells of candida guilliermondii immobilized onto porous glass spheres were cultured batchwise in a fluidized bed bioreactor for xylitol production from sugarcane bagasse hemicellulose hydrolyzate. an aeration rate of only 25 ml/min ensured minimum yields of xylose consumption (0.60) and biomass production (0.14 g(dm)/g(xyl)), as well as maximum xylitol yield (0.54 g(xyt)/g(xyl)) and ratio of immobilized to total cells (0.83). these results suggest that cell metabolism, although slow because of o ...	2003	12892483
prevalence of potentially pathogenic fungi in the bathing sites of the sulejów reservoir.	progressive degradation of water ecosystems may contribute to the propagation of fungi pathogenic to humans and animals. the aim of this study was a quantitative evaluation and identification of species of potentially pathogenic fungi in the littoral zone of the sulejów reservoir used as recreation site. we studied samples of surface water and sediments collected from 6 sampling sites of the reservoir in the years 2000 and 2001. in 2000, fungi were isolated from 82.7% of samples, while in 2001 f ...	2003	16889024
in vitro antifungal activities of anidulafungin and micafungin, licensed agents and the investigational triazole posaconazole as determined by nccls methods for 12,052 fungal isolates: review of the literature.	the echinocandins anidulafungin and micafungin and the triazole posaconazole are currently undergoing phase iii clinical trials. caspofungin and voriconazole have recently been licensed for the treatment of aspergillosis (both agents), other less common mould (voriconazole) and candidal (caspofungin) infections. this review summarizes the published in vitro data obtained by nccls or nccls modified methods on the in vitro fungistatic and fungicidal activities of these five agents for yeasts and m ...	2003	15456349
the distribution frequency of candida species in the genitourinary tract among symptomatic individuals in nigerian cities.	a clinical survey was carried out in seven cities in the southern part of nigeria to determine the relative distribution of genitourinary candida species in symptomatic patients reporting for diagnosis and treatment. seven candida species were identified using the chromagar candida method and the api 20c system. candida species were represented by candida glabrata (33.7%), candida albicans (20.1%), candida tropicalis (18%), candida guilliermondii (17.8%) candida pseudotropicalis (5%), candida pa ...	2003	15456373
[multifocal fungal infections in patients after renal transplantation undergoing immunosuppression].	the aim of the study was to estimate the prevalence of fungi in monofocal and multifocal infections in renal transplant recipients and the characteristics of 60 species and intraspecies features of fungal strains isolated from the organ ontocenoses: oral cavity, rectum and genital organs in 32 patients undergoing permanent immunosuppression after renal transplantation. fungal strains were recovered in 66 out of 96 samples (68.2% off all renal transplant recipients). there were in ontocenoses: or ...	2003	14974373
interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components.	the study considers an experimental model of the "autotroph-heterotroph" system with a closed atmosphere cycle, in which the heterotrophic link is a mixed yeast population. the autotrophic link is represented by the algae chlorella vulgaris and the heterotrophic link by the yeasts candida utilis and candida guilliermondii. the controls are populations of chlorella and the same yeasts isolated from the atmosphere. it has been shown that the outcome of competition in the heterotrophic link depends ...	2003	14503513
[the red mutations impair the regulation of flavinogenesis and metal homeostas in yeast pichia guilliermondii].	a method of positive selection of mutants with impaired regulation of flavinogenesis and metal homeostasis in yeast pichia guilliermondii was developed. this positive selection system was based on the isolation of pseudo-wild-type revertants (the rib+ phenotype) in riboflavin-dependent rib1-86 mutant (the rib- phenotype) of yeast p. guilliermondii. mutation rib1-86 blocks activity of the gtp cyclohydrolase ii catalyzing the first step in riboflavin (rf) biosynthesis. study of a collection of spo ...	2003	14515457
rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2.	a multiplex pcr method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. the species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rrna gene, whereas the universal primer was located at the 26s rrna gene. the eight species were candida albicans, candida glabrata, candida guilliermondii, candida krusei, candida lusitaniae, candida parapsilosis, candida tropical ...	2003	14557922
species identification of medically important fungi by use of real-time lightcycler pcr.	invasive fungal infection has become a major cause of morbidity and mortality in immunocompromised patients. rapid identification of pathogenic fungi to species level is critical for disease treatment. a real-time lightcycler assay aiming at rapid detection and species identification of pathogenic fungi from clinical isolates was developed. template dnas of different species were amplified and detected in real time by employing sybr green fluorescent dye. the target sequences for species-level d ...	2003	14614065
batch xylitol production by candida guilliermondii fti 20037 from sugarcane bagasse hemicellulosic hydrolyzate at controlled ph values.	batch fermentation of sugarcane bagasse hemicellulosic hydrolyzate by the yeast candida guilliermondii fti 20037 was performed using controlled ph values (3.5, 5.5, 7.5). the maximum values of xylitol volumetric productivity ( q(p)=0.76 g/l h) and xylose volumetric consumption ( q(s)=1.19 g/l h) were attained at ph 5.5. at ph 3.5 and 7.5 the q(p) value decreased by 66 and 72%, respectively. independently of the ph value, y(x/s) decreased with the increase in y(p/s) suggesting that the xylitol bi ...	2003	14624353
batch xylitol production from wheat straw hemicellulosic hydrolysate using candida guilliermondii in a stirred tank reactor.	batch production of xylitol from the hydrolysate of wheat straw hemicellulose using candida guilliermondii was carried out in a stirred tank reactor (agitation speed of 300 rpm, aeration rate of 0.6 vvm and initial cell concentration of 0.5 g l(-1)). after 54 h, xylitol production from 30.5 g xylose l(-1) reached 27.5 g l(-1), resulting in a xylose-to-xylitol bioconversion yield of 0.9 g g(-1) and a productivity of 0.5 g l(-1) h(-1).	2003	14677703
screening and typing of patagonian wine yeasts for glycosidase activities.	the purpose of this study was to select autochthonous glycosidase producer yeasts with potential use in industrial production of patagonian red wines.	2004	14678162
candida guilliermondii fungemia. to treat or not to treat.		2004	14758396
distribution and antifungal susceptibility of candida species causing candidemia from 1996 to 1999.	susceptibilities to amphotericin b and fluconazole of 383 candida species isolated from blood were determined. candida albicans was the most common species (55.6%), followed by candida parapsilosis (17.5%), candida tropicalis (16.5%), candida glabrata (5.2%), candida guilliermondii (2.3%), and others (2.9%). all but three isolates, candida ciferrii, c. tropicalis, and c. glabrata, one each, were susceptible to amphotericin b. a total of 367 (95.8%) and 15 (4.2%) isolates were susceptible and sus ...	2004	14761719
effect of acetic acid present in bagasse hydrolysate on the activities of xylose reductase and xylitol dehydrogenase in candida guilliermondii.	the first two steps in xylose metabolism are catalyzed by nad(p)h-dependent xylose reductase (xr) (ec 1.1.1.21) and nad(p)-dependent xylitol dehydrogenase (xdh) (ec 1.1.1.9), which lead to xylose-->xylitol-->xylulose conversion. xylitol has high commercial value, due to its sweetening and anticariogenic properties, as well as several clinical applications. the acid hydrolysis of sugarcane bagasse allows the separation of a xylose-rich hemicellulosic fraction that can be used as a substrate for c ...	2004	15107950
structure-activity relationships of derivatives of fusapyrone, an antifungal metabolite of fusarium semitectum.	fusapyrone (1) and deoxyfusapyrone (2) are two 3-substituted-4-hydroxy-6-alkyl-2-pyrones isolated from fusarium semitectum that have considerable antifungal activity against molds. because of their low zootoxicity and selective action they are potentially utilizable along with biocontrol yeasts for control of postharvest crop diseases. seven derivatives of 1 (3 and 5-10) and one derivative of 2 (4) were obtained by chemical modifications of the glycosyl residue, the 2-pyrone ring, the aliphatic ...	2004	15137845
chromium accumulation by living yeast at various environmental conditions.	yeast tolerance to cr (iii) and cr (vi) as well as chromium accumulation potential were shown to depend on treatment time, metal concentration, biomass density and the phase of growth. kinetic studies as exemplified by pichia guilliermondii atcc 201911 revealed a biphasic mode of cr (iii) uptake: a rapid sorption phase was followed by a slow process of accumulation, in which the contribution of the cell-bound cr fraction increased, while the total cellular cr level remained constant. cr (vi) upt ...	2004	15160602
optimized extraction by cetyl trimethyl ammonium bromide reversed micelles of xylose reductase and xylitol dehydrogenase from candida guilliermondii homogenate.	the intracellular enzymes xylose reductase (xr, ec 1.1.1.21) and xylitol dehydrogenase (xd, ec 1.1.1.9) from candida guilliermondii, grown in sugar cane bagasse hydrolysate, were separated by reversed micelles of cetyl trimethyl ammonium bromide (ctab) cationic surfactant. an experimental design was employed to optimize the extraction conditions of both enzymes. under these conditions (temperature = 5 degree c, hexanol: isooctane proportion = 5% (v/v), 22 %, surfactant concentration = 0.15m, ph ...	2004	15177159
liquid-liquid extraction of xylitol dehydrogenase from candida guilliermondii homogenate by reversed micelles.	the intracellular enzyme xylitol dehydrogenase (xd, ec 1.1.1.9) from candida guilliermondii, grown in sugarcane bagasse hydrolysate, was separated by reversed micelles of bdbac [n-benzyl-n-dodecyl-n-bis (2-hydroxyethyl) ammonium chloride] cationic surfactant. an experimental design was employed to evaluate the influence of the following factors on the enzyme separation: temperature, co-solvent concentration and surfactant concentration. the results showed that just the temperature did not show s ...	2004	15177160
phenotypic and genotypic identification of candida spp. isolated from hospitalized patients.	as candidosis incidence continue to rise, quick laboratory identification of candida species is becoming increasingly important for a growing population of patients at-risk. rapd techniques were used on samples of candida obtained from patients hospitalized at santa casa de misericordia in belo horizonte (scmbh) brazil, from march 1998 to december 2000 and then compared with the results of phenotypic identification techniques. two hundred and forty two yeasts were isolated and phenotypically ide ...	2004	15458359
three new beetle-associated yeast species in the pichia guilliermondii clade.	new yeasts in the pichia guilliermondii clade were isolated from the digestive tract of basidiocarp-feeding members of seven families of coleoptera. a molecular phylogeny and unique traits placed eight isolates in candida fermentati and three undescribed taxa in the genus candida. the new species and type strains are c. smithsonii (type strain nrrl y-27642t), c. athensensis (type strain nrrl y-27644t), and c. elateridarum (type strain nrrl y-27647t). based on comparison of small-and large-subuni ...	2004	15381126
in vitro pharmacodynamic characteristics of amphotericin b, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent candida species from patients with hematologic malignancies.	time-kill and postantifungal effect (pafe) of amphotericin b, caspofungin, fluconazole, and voriconazole were determined against clinical isolates of candida guilliermondii, candida kefyr, and candida lusitaniae. azoles displayed fungistatic activity and no measurable pafe, regardless of the concentration tested. amphotericin b and caspofungin demonstrated concentration-dependent fungicidal activity, although amphotericin b only produced a significant dose-dependent pafe against all isolates tes ...	2004	15504881
survey of yeasts for antagonistic activity against salmonella poona in cantaloupe juice and wounds in rinds coinfected with phytopathogenic molds.	application of yeasts as biocontrol agents to prevent mold decay of fruits and vegetables has been described. we examined 10 yeasts for potential antagonistic activity against survival and growth of salmonella poona in cantaloupe juice and decay by cladosporium cladosporioides and geotrichum candidum in wounds on cantaloupe rind. cantaloupe juice was inoculated using five schemes: salmonella poona only (1.10 log cfu/ml), high (3.93 to 5.21 log cfu/ml) or low populations (1.79 to 3.26 log cfu/ml) ...	2004	15508621
in-vitro activity of 5-fluorocytosine against 1,021 spanish clinical isolates of candida and other medically important yeasts.	the aim of this study was to determine the prevalence of primary resistance to 5-fluorocytosine (5fc) among clinical isolates of yeasts in spain where this drug is not currently available for therapy. we have tested the in vitro activity of 5fc against 1,021 recent yeast clinical isolates, including 522 candida albicans, 140 candida parapsilosis, 68 candida glabrata, 41 candida dubliniensis, 50 candida guilliermondii, 34 candida tropicalis, 28 candida krusei, 20 candida famata, 11 cryptococcus n ...	2004	15538829
candida famata (debaryomyces hansenii) dna sequences containing genes involved in riboflavin synthesis.	previously cloned candida famata (debaryomyces hansenii) strain vkm y-9 genomic dna fragments containing genes rib1 (codes for gtp cyclohydrolase ii), rib2 (encodes specific reductase), rib5 (codes for dimethylribityllumazine synthase), rib6 (encodes dihydroxybutanone phosphate synthase) and rib7 (codes for riboflavin synthase) were sequenced. the derived amino acid sequences of c. famata rib genes showed extensive homology to the corresponding sequences of riboflavin synthesis enzymes of other ...	2004	15543522
initial results from a longitudinal international surveillance programme for anidulafungin (2003).	this longitudinal study evaluated the in vitro activity of anidulafungin against 880 clinical yeast isolates and 68 mould isolates from 64 medical centres in north america, latin america and europe.	2004	15564286
phylogeny and evolution of medical species of candida and related taxa: a multigenic analysis.	hemiascomycetes are species of yeasts within the order saccharomycetales. the order encompasses disparate genera with a variety of life styles, including opportunistic human pathogens (e.g., candida albicans), plant pathogens (e.g., eremothecium gossypii), and cosmopolitan yeasts associated with water and decaying vegetation. to analyze the phylogeny of medically important species of yeasts, we selected 38 human pathogenic and related strains in the order saccharomycetales. the dna sequences of ...	2004	15583292
[cloning of structural genes involved in riboflavin synthesis of the yeast candida famata].	the riboflavin overproducing mutants of the flavinogenic yeast candida famata isolated by conventional selection methods are used for the industrial production of vitamin b2. recently, a transformation system was developed for c. famata using the leu2 mutant as a recipient strain and saccharomyces cerevislae leu2 gene as a selective marker. in this paper the cloning of c. famata genes for riboflavin synthesis on the basis of developed transformation system for this yeast species is described. ri ...	2004	15909421
pyridoxal 5'-phosphate inactivates dna topoisomerase ib by modifying the lysine general acid.	the present results demonstrate that pyridoxal, pyridoxal 5'-phosphate (plp) and pyridoxal 5'-diphospho-5'-adenosine (plp-amp) inhibit candida guilliermondii and human dna topoisomerases i in forming an aldimine with the epsilon-amino group of an active site lysine. plp acts as a competitive inhibitor of c.guilliermondii topoisomerase i (k(i) = 40 microm) that blocks the cleavable complex formation. chemical reduction of plp-treated enzyme reveals incorporation of 1 mol of plp per mol of protein ...	2004	15494452
[study of in vitro activity of caspofungin on non-candida albicans yeast strains determined by two methods: m27-a2 and eucast].	the in vitro activity of caspofungin against 147 non-candida albicans yeasts isolated from blood culture was studied using two broth microdilution methods: m27-a2 and eucast. the minimum concentrations that produced a growth inhibition of > or = 50% and of 100% (mic2 and mic0, respectively) and a reduction in the number of viable colonies > or = 99% versus the initial inoculum (mfc) were determined for all strains. caspofungin demonstrated good activity (mic2 and mic0 < or = 2 mg/l for 90% of th ...	2004	15619655
two new species in the pichia guilliermondii clade: pichia caribbica sp. nov., the ascosporic state of candida fermentati, and candida carpophila comb. nov.	pichia caribbica sp. nov. (type strain dbvpg 4519, nrrl y-27274, cbs 9966) is described as the ascosporic state of candida fermentati, and candida guilliermondii var. carpophila (type strain dbvpg 7739, nrrl y-17905, cbs 5256) is elevated to species status as candida carpophila comb. nov. these new taxa, which are indistinguishable on the basis of conventional taxonomic criteria, differ from one another and from pichia guilliermondii by low dna base sequence relatedness, different electrophoreti ...	2005	15691751
mycological examinations on the fungal flora of the chicken comb.	a total of 500 combs of adult chickens from two different locations in germany (hessen and schleswig-holstein) were clinically and mycologically examined. the chickens came from three battery cages (n = 79), one voliere system (n=32), six flocks maintained on deep litter (n = 69) and 12 flocks kept on free outdoor range (n=320). twenty-two of the 500 chicken combs (4.4%) were found to have clinical signs: only non-specific lesions neither typical of mycosis nor of avian pox such as desquamation ...	2005	15743428
yeast diversity in hypersaline habitats.	thus far it has been considered that hypersaline natural brines which are subjected to extreme solar heating, do not contain non-melanized yeast populations. nevertheless we have isolated yeasts in eight different salterns worldwide, as well as from the dead sea, enriquillo lake (dominican republic) and the great salt lake (utah). among the isolates obtained from hypersaline waters, pichia guilliermondii, debaryomyces hansenii, yarrowia lipolytica and candida parapsilosis are known contaminants ...	2005	15766773
two new fungal inteins.	until recently the only intein known to be encoded by the nuclear genome of a eukaryote was the vma intein in the vacuolar atpase precursor of several species of saccharomycete yeast. this intein has been intensively studied and much information has been gained about its structure, mode of action and evolutionary history. we recently reported a second nuclear intein, cne prp8, encoded within the prp8 gene of the basidiomycete cryptococcus neoformans. subsequent studies have found allelic prp8 in ...	2005	15849795
vulvovaginal candidiasis in a flemish patient population.	increased resistance to fluconazole has been reported in oral, oesophageal and urinary candida isolates, but this has not been observed commonly in genital tract isolates. the rate of isolation of candida spp. and their susceptibility to amphotericin b, flucytosine and azoles were determined in a number of clinical practices in the city of ghent, belgium. patients with symptomatic vulvovaginal candidiasis (vvc) were treated with fluconazole, and the mycological and clinical outcomes were evaluat ...	2005	16307555
occurrence and diversity of yeasts in the mid-atlantic ridge hydrothermal fields near the azores archipelago.	the yeast community associated with deep-sea hydrothermal systems of the mid-atlantic rift was surveyed for the first time. this study relied on a culture-based approach using two different growth media: a conventional culture medium for yeasts supplemented with sea salts (mypss) and the same medium additionally supplemented with sulfur (mypsss). for the evaluation of species diversity, a molecular approach involving minisatellite-primed polymerase chain reaction (msp-pcr) strain typing and sequ ...	2005	16328655
yeast community survey in the tagus estuary.	the yeast community in the waters of the tagus estuary, portugal, was followed for over a year in order to assess its dynamics. yeast occurrence and incidence were measured and this information was related to relevant environmental data. yeast occurrence did not seem to depend upon tides, but river discharge had a dramatic impact both on the density and diversity of the community. the occurrence of some yeasts was partially correlated with faecal pollution indicators. yeast isolates were charact ...	2005	16329949
evaluation of inoculum of candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate.	the effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/l), xylose (30 g/l), or a mixture of glucose (2 g/l) and xylose (30 g/l). the inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 iu/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0 ...	2005	15917619
sugarcane bagasse as raw material and immobilization support for xylitol production.	xylose-to-xylitol bioconversion was performed utilizing candida guilliermondii immobilized in sugarcane bagasse and cultured in erlenmeyer flasks using sugarcane bagasse hydrolysate as the source of xylose. fermentations were carried out according to a factorial design, and the independent variables considered were treatment, average diameter, and amount of bagasse used as support for cell immobilization. by increasing the amount of support, the xylitol yield decreased, whereas the biomass yield ...	2005	15920271
optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by candida guilliermondii.	the dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/l of xylose, which were fermented with a high inoculum concentration (10 g/l of the yeast candida guilliermondii im/ufrj 50088). the addition of urea reduced the time of conversion (tc) to 75 h (without nitrogen source addition tc > 127 h), and, consequently, improving the rates of xylitol bioproduction. fermentator experiments, using the optimized co ...	2005	15920277
positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast pichia guilliermondii.	it is known for many years that iron represses synthesis of riboflavin (rf) and most of rf-synthesizing enzymes in several yeast species, known as flavinogenic yeasts. however, the mechanism of such repression is not known. we have found that iron represses transcription of rib1 and rib7 genes coding for the first and the last enzymes of rf biosynthesis in the model flavinogenic organism pichia guilliermondii. to decipher molecular mechanisms of iron-dependent repression, isolation and study of ...	2005	15925311
a surprisingly large rnase p rna in candida glabrata.	we have found an extremely large ribonuclease p (rnase p) rna (rpr1) in the human pathogen candida glabrata and verified that this molecule is expressed and present in the active enzyme complex of this hemiascomycete yeast. a structural alignment of the c. glabrata sequence with 36 other hemiascomycete rnase p rnas (abbreviated as p rnas) allows us to characterize the types of insertions. in addition, 15 p rna sequences were newly characterized by searching in the recently sequenced genomes cand ...	2005	15987816
kinetic behavior of candida guilliermondii yeast during xylitol production from brewer's spent grain hemicellulosic hydrolysate.	brewer's spent grain, the main byproduct of breweries, was hydrolyzed with dilute sulfuric acid to produce a hemicellulosic hydrolysate (containing xylose as the main sugar). the obtained hydrolysate was used as cultivation medium by candidaguilliermondii yeast in the raw form (containing 20 g/l xylose) and after concentration (85 g/l xylose), and the kinetic behavior of the yeast during xylitol production was evaluated in both media. assays in semisynthetic media were also performed to compare ...	2005	16080723
malassezia and candida colonisation on glans penis of circumcised men.	the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ...	2005	16115108
purification of xylitol from fermented hemicellulosic hydrolyzate using liquid-liquid extraction and precipitation techniques.	xylitol was produced by candida guilliermondii by fermentation of sugarcane bagasse hemicellulosic hydrolysate. undesirable impurities were extracted from the broth using either ethyl acetate, chloroform or dichloromethane. the best results on clarification of the broth without xylitol loss were obtained with ethyl acetate. when ethanol, acetone or tetrahydrofuran were used for precipitation of impurities, only tetrahydrofuran clarified the fermented broth, but a high xylitol loss (approximately ...	2005	16132861
development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses.	invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ...	2005	16207946
candida guilliermondii isolated from hiv-infected human secretes a 50 kda serine proteinase that cleaves a broad spectrum of proteinaceous substrates.	non-albicans candida species cause 35-65% of all candidemias in the general population, especially in immunosuppressed individuals. here, we describe a case of a 19-year-old hiv-infected man with pneumonia due to a yeast-like organism. this clinical yeast isolate was identified as candida guilliermondii through mycological tests. c. guilliermondii was cultivated in brain heart infusion medium for 48 h at 37 degrees c. after sequential centrifugation and concentration steps, the free-cell culture ...	2005	15607631
[selection and properties of mutant yeast pichia guilliermondii strains resistant to chromium (vi)].	yeast pichia guilliermondii strains l3 and l2, exposed to uv mutagenesis, produced over 80 mutants capable of growing on media containing 1.5 mm bichromate (cr(vi)). the mutations making the strains resistant to cr(vi) were dominant or semidominant. the mutants varied in cr(vi) resistance, the degree of chromium accumulation in the cells (from 0.1 to 11.6 mg/g dry cells), and the degree of cr(vi) reduction (from 50% to complete disappearance of bichromate from the culture liquid). chromium accum ...	2005	15859465
disseminated infection due to candida guilliermondii in a patient with aml(m4). case study.	a 43-year-old patient admitted with acute myelogenous leukemia, developed bronchopneumonia and sepsis during profound neutropenia. fever and pulmonary infiltrates did not improve by using empiric antibacterial therapy (cefoperazona-sulbactam, trimethoprim-sulphametoxazol). blood and sputum culture were performed and patient received voriconazol. both cultures, from blood and sputum, yielded candida guilliermondii after 48 hours of incubations. the isolates have the same biochemical and antimicro ...	2006	17571574
a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis.	to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available.	2006	17121679
[susceptibility of fungi isolated from clinical materials to voriconazole].	the objective of the study was evaluation of the susceptibility of 139 fungal strains isolated from clinical materials to voriconazole, a new antifungal agent of the triazole group. a dilution method was used. the drug was incorporated into the culture medium at concentrations 0.1-100 mg/l. it was found out that the antifungal effectiveness of the drug varied both between various fungal genera and species, and between strains within the same species. total inhibition of the growth of 20% of the ...	2006	17133909
molecular typing of the yeast species dekkera bruxellensis and pichia guilliermondii recovered from wine related sources.	a total of 63 strains of dekkera bruxellensis and 32 strains of pichia guilliermondii isolated from wine related environments were identified by restriction analysis of the 5.8s-its region of the rdna. these strains were subjected to intraspecific discrimination using mtdna restriction and rapd-pcr analysis. the isolates identified as d. bruxellensis yielded 3 different molecular patterns of mtdna restriction using the endonuclease hinfi. the pattern a was the most frequent (58 strains) among st ...	2006	16229917
fermentation performance of candida guilliermondii for xylitol production on single and mixed substrate media.	semidefined media fermentation simulating the sugar composition of hemicellulosic hydrolysates (around 85 g l(-1) xylose, 17 g l(-1) glucose, and 9 g l(-1) arabinose) was investigated to evaluate the glucose and arabinose influence on xylose-to-xylitol bioconversion by candida guilliermondii. the results revealed that glucose reduced the xylose consumption rate by 30%. arabinose did not affect the xylose consumption but its utilization by the yeast was fully repressed by both glucose and xylose ...	2006	16541249
occurrence of yeasts in cloacae of migratory birds.	several species of yeast have been reported as pathogens in humans based on increases in immunodeficiency syndromes and as a result of immunosuppressant chemotherapy in cancer treatment. domestic and wild birds are known to act as carriers of human pathogenic fungi. to gain additional information on the yeasts present in the cloacae of some species of migratory birds, 421 wild birds (24.39% out of 1726 birds caught in romania, hungary and bulgaria) were sampled with the permission of the local j ...	2006	16552486
effect of phosphate buffer concentration on the batch xylitol production by candida guilliermondii.	to evaluate the effect of phosphate buffer concentration on growth and xylitol production by candida guilliermondii fti 20037.	2006	16599982
in vitro anticandidal activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb.	xanthorrhizol, isolated from the methanol extract of curcuma xanthorrhiza roxb., was investigated for its anticandidal activity using six candida species.	2006	16617064
multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen candida guilliermondii.	candida guilliermondii is a haploid opportunistic pathogen accounting for about 2 % of human blood yeast infections. recent analyses using multilocus enzyme electrophoresis and karyotyping suggest that strains from human sources traditionally designated c. guilliermondii in fact include at least two species, c. guilliermondii and candida fermentati. however, the patterns of molecular variation within and between these two species remain largely unknown. in this study, dna fragments were sequence ...	2006	16622071
enhanced xylitol production by precultivation of candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate.	the present work evaluated the key enzymes involved in xylitol production (xylose reductase [xr] and xylitol dehydrogenase [xdh]) and their correlation with xylose, arabinose, and acetic acid assimilation during cultivation of candida guilliermondii fti 20037 cells in sugarcane bagasse hemicellulosic hydrolysate. for this purpose, inocula previously grown either in sugarcane bagasse hemicellulosic hydrolysate (sbhh) or in semidefined medium (xylose as a substrate) were used. the highest xylose/a ...	2006	16775788
candida guilliermondii fungemia in patients with hematologic malignancies.	the microbiological, clinical, and epidemiological features of most non-candida albicans candida species are well known, but much less is known about species such as candida guilliermondii, an uncommon pathogen causing a variety of deep-seated infections in immunocompromised hosts. to characterize c. guilliermondii fungemia in patients with hematological malignancies and its susceptibility to antifungal drugs, all cases of c. guilliermondii fungemia diagnosed in our department between 1983 and 2 ...	2006	16825364
candida guilliermondii as the aetiology of candidosis.	candida guilliermondii is one of the components of human microbiota. this yeast has been infrequently associated with human infections, which may be related to its low pathogenicity. the aim of this study was to provide clinical and epidemiological data for patients infected with c. guilliermondii at santa casa complexo hospitalar, brazil. from october 1997 to october 2003, c. guilliermondii was isolated from clinical samples from 11 patients. three patients were excluded because the isolation o ...	2006	16847499
survey of amphotericin b susceptibility of candida clinical isolates determined by etest.	the minimal inhibitory concentrations (mics) of amphotericin b (amb) determined by the national committee for clinical laboratory standards (nccls; nccls document m27-a) broth dilution method are in a relatively narrow ranges and this may lead to underestimation of the amb-resistant rate in clinical isolates. we evaluated in vitro susceptibility of clinical isolates of candida spp. to amb using etest and determined the distribution of amb mics in different species.	2006	16926981
application of pcr-dgge to analyse the yeast population dynamics in slurry reactors during degradation of polycyclic aromatic hydrocarbons in weathered oil.	slurry-phase reactors have been used to investigate the biodegradation feasibility of polycyclic aromatic hydrocarbons (pahs) in weathered crude oil, by mixed culture containing five pahs-degrading yeast strains. yeasts were isolated from the oily soil by enrichment culture, using phenanthrene as a sole carbon source, and identified based on the 26s ribosomal dna (rdna) sequence. yeast strains belonged to the genera candida, pichia, rhodotorula and sporidiobolus. the experiment was carried out f ...	2006	17001616
determination of mics of aminocandin for candida spp. and filamentous fungi.	candida and aspergillus spp., as well as other filamentous molds, have increasingly been reported as the causes of severe invasive fungal infections. we evaluated the new echinocandin aminocandin (amn) for its antifungal activities against a range of fungal pathogens by determination of the mics for the organisms. the mics of the comparator drugs amphotericin b, caspofungin, micafungin, and voriconazole were also determined. the mics of amn for 25 strains each of non-candida albicans candida spp ...	2006	17021057
global surveillance of in vitro activity of micafungin against candida: a comparison with caspofungin by clsi-recommended methods.	micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive candida sp. isolates is indicated. we determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of candida spp. collected from 60 medical centers worldwide in ...	2006	17021079
candida guilliermondii, an opportunistic fungal pathogen with decreased susceptibility to fluconazole: geographic and temporal trends from the artemis disk antifungal surveillance program.	although a rare cause of invasive candidiasis, candida guilliermondii has been reported to exhibit decreased susceptibility to antifungal agents. aside from case reports and small surveys, there is little information regarding the epidemiology and antifungal susceptibility profile of c. guilliermondii. we report geographic and temporal trends in the isolation and antifungal susceptibilities of 1,029 c. guilliermondii clinical isolates collected from 127 medical centers as part of the artemis dis ...	2006	17021081
screening and characterization of yeasts for xylitol production.	to discover novel naturally occurring xylitol producing yeast species with potential for industrial applications.	2006	17040233
effects of caspofungin against candida guilliermondii and candida parapsilosis.	the in vitro activity of caspofungin (cas) was investigated against 28 yeast isolates belonging to candida albicans (n = 5), candida guilliermondii (n = 10), and candida parapsilosis (n = 13). cas mics obtained by broth dilution and etest methods clearly showed a rank order of susceptibility to the echinocandin compound with c. albicans > c. parapsilosis > c. guilliermondii. similarly, time-kill assays performed on selected isolates showed that cas was fungistatic against c. albicans and c. para ...	2006	16870764
killing kinetics of caspofungin, micafungin, and amphotericin b against candida guilliermondii.	amphotericin b (amb), micafungin, and caspofungin mics, minimal fungicidal concentrations, and time-killing curves were determined in the presence and absence of 10% inactivated serum. amb was the only agent with consistent killing activity (time required to achieve 99.9% of growth reduction was 2.1 to 3.2 h). the presence of serum enhanced caspofungin activity but lowered those of micafungin and amb.	2006	16870779
candida albicans and candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility.	a recognized hotspot for mutations conferring reduced echinocandin susceptibility (res) is residue s645 of candida albicans gsc1(fks1). we report that the mutation f641y is associated with res in a c. albicans isolate. the analogous fks2 residue is mutated f to v in a candida glabrata res isolate; the introduction of this mutation into susceptible c. glabrata confirmed its role in res. y641-equivalent fks residues were identified in intrinsically res fusarium species and candida guilliermondii.	2006	16870797
human recombinant antimannan immunoglobulin g1 antibody confers resistance to hematogenously disseminated candidiasis in mice.	mannan is a major cell wall component found in candida species. natural antimannan antibody is present in sera from most normal adults, but its role in host resistance to hematogenously disseminated candidiasis is unknown. the purpose of this study was to develop recombinant human antimannan antibody and to study its protective function. a phage fab display combinatorial library containing fab genes from bone marrow lymphocytes was screened with candida albicans yeast cells and chemically purifi ...	2006	16368991
structural changes of cell wall mannans of candida guilliermondii ifo 10279 strain cells cultured at high temperature.	the morphology, structure, and antigenicity of the cells and the cell wall mannans of the candida guilliermondii ifo 10279 strain cultivated at 33 and 34 degrees c for 48 h in yeast extract-added sabouraud liquid medium (yslm) were compared with those cultivated at 27 degrees c and 33 degrees c and then at 27 degrees c (33-27 degrees c). this strain showed little growth at higher than 35 degrees c. the density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 degree ...	2006	16462053
application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses.	bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. the diversity and succession of yeast populations in three traditional registered designation of origin (r.d.o.) salers cheeses have been determined by using phenotypic diagnoses and single-strand conformation polymorphism (sscp) analysis. isolates were identified by phenotypic tests and the sequencing of the d1-d2 domains of the 26s rrna gene. ninety- ...	2006	16464699
rapid identification of fungal pathogens in bact/alert, bactec, and bbl mgit media using polymerase chain reaction and dna sequencing of the internal transcribed spacer regions.	we report a direct polymerase chain reaction/sequence (d-pcrs)-based method for the rapid identification of clinically significant fungi from 5 different types of commercial broth enrichment media inoculated with clinical specimens. media including bact/alert fa (biomérieux, marcy l'etoile, france) (n = 87), bactec plus aerobic/f (becton dickinson, microbiology systems, sparks, md) (n = 16), bactec peds plus/f (becton dickinson) (n = 15), bactec lytic/10 anaerobic/f (becton dickinson) (n = 11) b ...	2006	16466900
rsm analysis of the effects of the oxygen transfer coefficient and inoculum size on the xylitol production by candida guilliermondii.	biotechnology production of xylitol is an excellent alternative to the industrial chemical process for the production of this polyalcohol. in this work the behavior of candida guilliermondii yeast was studied when crucial process variables were modified. the k(l)a (between 18 and 40/h) and the initial cell mass (between 4 and 10 g) were considered as control variables. a response surface methodology was applied to the experimental design to study the resulting effect when the control variables w ...	2006	16915645
inulinase production by a marine yeast pichia guilliermondii and inulin hydrolysis by the crude inulinase.	marine yeast strain 1, isolated from the surface of a marine alga, was found to secrete a large amount of inulinase into the medium. this marine yeast was identified as a strain of pichia guilliermondii according to the results of routine yeast identification and molecular methods. the crude inulinase produced by this marine yeast worked optimally at ph 6.0 and 60 degrees c. the optimal medium for inulinase production was seawater containing 4.0% (w/v) inulin and 0.5% (w/v) yeast extract, while ...	2007	17111131