Publications

tissue distribution, synthesis stage, and ethylene induction of pineapple (ananas comosus) chitinases.	we examined the tissue distribution, synthesis stage, and ethylene induction of three types of pineapple chitinase using chitinase activity gel and immunoblot analysis. type a (acidic class iii) exists in all tissues, while type b (weakly basic class i, which has strong antifungal activity) and type c (acidic class i) are localized mainly in the leaf and stem. in a pericarp, type a exists at all stages during fruit development, while type b and type c exist only at the early stage. synthesis of ...	2005	15849431
structure-function relationship of bromelain isoinhibitors from pineapple stem.	bromelain isoinhibitors from pineapple stem (bis) are unique double-chain inhibitors and inhibit the cysteine proteinase bromelain competitively. the three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded anti-parallel beta-sheet. unexpectedly, bis were found to share similar folding and disulfide-bond connectivities not with the cystatin superfamily, but with bowman-birk trypsin/chymotrypsin inhibitor (bbi). the structural simil ...	2005	12437100
bromelain, the enzyme complex of pineapple (ananas comosus) and its clinical application. an update.	after a short description of the uses of pineapple as folk medicine by the natives of the tropics, the more important new pharmaceutical applications of bromelain, reported between 1975 and 1978, are presented. although the exact chemical structure of all active components of bromelain is not fully determined, this substance has shown distinct pharmacological promise. its properties include: (1) interference with growth of malignant cells; (2) inhibition of platelet aggregation; (3) fibrinolytic ...	2005	3287010
[latex allergy in children: description of two cases].	here we present two cases of latex hypersensitivity. the clinical manifestations were conjunctivitis, urticaria, angioedema and dermatitis. the patients presented positive skin prick test (spt) to latex with a commercial extract and by pricking through a latex surgical glove. radioallergosorbent test (rast) to latex and patch testing to common additives and to latex were negative. skin prick tests with fruits (banana, kiwi, pineapple, apricot, avocado, grape) were positive but children presented ...	2006	7610085
dermatological hazards of the pineapple industry.		2006	13428186
effects of oral bromelain administration on the impaired immunocytotoxicity of mononuclear cells from mammary tumor patients.	the protease bromelain from pineapple was suggested for adjuvant therapy of malignant diseases. we studied immunological effects of an orally applied bromelain drug on 16 breast cancer patients in comparison with healthy donors. bromelain was applied for 10 days with a daily dose of 3000 f.i.p. units and the immunocytotoxicity of blood monocytes and lymphocytes against the leukemic k562 and mda-mb-231 mammary carcinoma target cells was determined in vitro. in addition, the expression of the cell ...	2006	10523679
bromelain as an adjunctive treatment for moderate-to-severe osteoarthritis of the knee: a randomized placebo-controlled pilot study.	osteoarthritis (oa) of the knee is the most prevalent joint disorder. previous studies suggest that bromelain, a pineapple extract, may be a safer alternative/adjunctive treatment for knee oa than current conventional treatment.	2006	17121765
cine magnetic resonance imaging of the small bowel: comparison of different oral contrast media.	to evaluate several substances regarding small bowel distension and contrast on balanced steady-state free precession (bssfp) cine magnetic resonance (mr) images.	2006	17077038
deforestation alters phytotelm habitat availability and mosquito production in the peruvian amazon.	we quantified the effects of deforestation, and subsequent cultivation and forest regeneration, on the abundance and composition of mosquito larval habitats, specifically phytotelmata (plant-held waters), in the western amazon basin. recently deforested sites were characterized by increased phytotelm density (1.6 phytotelmata/m2) and greater relative abundance of fallen-plant-part phytotelmata (76%) compared to intact forests (0.9 phytotelmata/m2 and 25% fallen plant parts). as a result, the tot ...	2006	17069377
[studies on phenolic constituents from leaves of pineapple (ananas comosus)].	to study the phenolic constituents of the leaves of pineapple.	2006	17048566
silencing of the acc synthase gene acacs2 causes delayed flowering in pineapple [ananas comosus (l.) merr.].	flowering is a crucial developmental stage in the plant life cycle. a number of different factors, from environmental to chemical, can trigger flowering. in pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environmental cues. a 1-amino-cyclopropane-1-carboxylate synthase (acc synthase) gene has been cloned from pineapple (acacs2), which is induced in the meristem under the same environmental c ...	2006	17046980
the complete chloroplast genome sequence of citrus sinensis (l.) osbeck var 'ridge pineapple': organization and phylogenetic relationships to other angiosperms.	the production of citrus, the largest fruit crop of international economic value, has recently been imperiled due to the introduction of the bacterial disease citrus canker. no significant improvements have been made to combat this disease by plant breeding and nuclear transgenic approaches. chloroplast genetic engineering has a number of advantages over nuclear transformation; it not only increases transgene expression but also facilitates transgene containment, which is one of the major impedi ...	2006	17010212
determination of isopropyl thioxanthone (itx) in fruit juices by pressurized liquid extraction and liquid chromatography-mass spectrometry.	a rapid lc-ms method, which compares different mass analyzers-single quadrupole, ion trap, and triple quadrupole-was developed for the quantitative determination of isopropyl thioxanthone (itx) in fruit juices. itx, a photoinitiator in uv-cured inks that can reach foods from the cartons for beverages in which they are used, was extracted from fruit juice samples with acetone/hexane (50:50) using pressurized liquid extraction. this method gave detection limits of 3, 3, and 0.01 microg/l and quant ...	2006	17002475
bioaffinity based oriented immobilization of stem bromelain.	bromelain is a basic, 23.8 kda thiol proteinase obtained from stem of the pineapple plant (ananas comosus) and is unique in containing a single oligosaccharide chain attached to the polypeptide. this property allowed its affinity binding and favorable orientation on a sepharose support pre-coupled with the lectin, concanavalin a (con a). for comparison, bromelain was also immobilized by covalently coupling to the cnbr-activated sepharose. the preparation obtained was more resistant to thermal in ...	2006	16786278
characterization of aroma compounds of chinese "wuliangye" and "jiannanchun" liquors by aroma extract dilution analysis.	aroma compounds in chinese "wuliangye" liquor were identified by gas chromatography-olfactometry (gc-o) after fractionation. a total of 132 odorants were detected by gc-o in wuliangye liquor on db-wax and db-5 columns. of these, 126 aromas were identified by gc-mass spectrometry (ms). aroma extract dilution analysis (aeda) was further employed to identify the most important aroma compounds in "wuliangye" and "jiannanchun" liquors. the results showed that esters could be the most important class, ...	2006	16569063
proliferation potential of 18-month-old callus of ananas comosus l. cv. moris.	differential effect of plant growth regulators and additives in proliferation of 18-month-old calli of ananas comosus l. cv. moris were assessed in vitro. the proliferation of callus relied on the growth regulators and additives. of the different auxins supplemented in the murashige and skoog (ms) media, 32.22 microm alpha-naphthaleneacetic acid (naa) gave the highest mean fresh weight of callus (46.817 g). medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-d) was inferior to naa, whil ...	2006	16493521
does modification of olfacto-gustatory stimulation diminish sensory-specific satiety in humans?	alimentary sensory pleasure is an important factor in ingestive behavior. renewal of olfacto-gustatory pleasure by introducing new foods or through seasoning of previously consumed food might increase intake.	2006	16458336
characterization of the acidic and basic limbs of a bell-shaped ph profile in the inhibitory activity of bromelain inhibitor vi.	bromelain inhibitor vi (bi-vi) is a cysteine proteinase inhibitor from pineapple stem and a unique two-chain inhibitor composed of two distinct domains. bi-vi's inhibitory activity toward the target enzyme bromelain is maximal at ph 4 and shows a bell-shaped ph profile with pka values of about 2.5 and 5.3. this ph profile is quite different from that of bromelain, which is optimally active around ph 7. in the present article, to characterize the acidic limb, we first expressed the recombinant in ...	2006	16315142
the influence of bromelain on platelet count and platelet activity in vitro.	bromelain is a general name for a family of sulfhydryl-containing, proteolytic enzymes from the pineapple plant. the aim of the present study was to investigate the influence of bromelain on platelet count, platelet aggregation and platelet activity in vitro. blood samples were taken from the antecubital vein of 10 healthy male non-smokers. platelet count decreased after incubation with 2.5 and 5 mg bromelain/ml from 277 +/- 17 platelets/nl before to 256 +/- 21 and 247 +/- 19 platelets/nl after ...	2006	16308185
therapeutic use, efficiency and safety of the proteolytic pineapple enzyme bromelain-pos in children with acute sinusitis in germany.	the therapeutic efficiency and safety of the proteolytic enzyme bromelaine obtained from pineapple (bromelain-pos, ursapharm gmbh, saarbrücken, germany) was evaluated in children under the age of 11 years diagnosed with acute sinusitis. data from 116 patients from 19 centres located across germany were analysed in a pharmacoepidemiological cohort study. patient cohorts were either treated with bromelain-pos (n = 62), in combination with bromelain-pos and standard therapies (n = 34), or with stan ...	2006	15796206
herbal remedy in the treatment of malaria: cross sectional survey of residents of lagos state, nigeria.	semi structured questionnaires. designed to capture information on the type. composition, method of preparation. dosage, mode of administration. and frequency of use of herbal preparations in malaria treatment, were administered to 1,593 adults of the 3 main ethnic groups and a forth group comprising other smaller ethnic groups designated as "others", all resident in lagos metropolis in a cross sectional survey. the 1,593 respondents were made up of 892 males and 701 females and their ages range ...	2006	17209310
fusarium-induced diseases of tropical, perennial crops.	abstract the world's oldest ecosystems are found in the tropics. they are diverse, highly evolved, but barely understood. this and subsequent papers describe diseases of tropical, perennial plants that are caused by fusarium spp. many of these are economically significant, difficult to manage, and of scientific interest. some represent coevolved patho-systems (e.g., panama disease, tracheomycosis of coffee, fusariosis of pineapple, and fusarium wilt of oil palm), whereas others may be new-encoun ...	2006	18943183
quality changes and nutrient retention in fresh-cut versus whole fruits during storage.	the influences of processing and storage on the quality indices and nutritional content of fresh-cut fruits were evaluated in comparison to whole fruits stored for the same duration but prepared on the day of sampling. fresh-cut pineapples, mangoes, cantaloupes, watermelons, strawberries, and kiwifruits and whole fruits were stored for up to 9 days in air at 5 degrees c. the postcutting life based on visual appearance was shorter than 6 days for fresh-cut kiwifruit and shorter than 9 days for fr ...	2006	16756358
effects of inducers of systemic acquired resistance on reproduction of meloidogyne javanica and rotylenchulus reniformis in pineapple.	the potency of the inducers of systemic acquired resistance (sar), acibenzolar-s-methyl, dl-alpha-amino-n-butyric acid (aaba), dl-beta-amino-n-butyric acid (baba), gamma-amino-n-butyric acid (gaba), p-aminobenzoic acid (paba), riboflavin, and salicylic acid (sa), in reducing reproduction of meloidogyne javanica and rotylenchulus reniformis in pineapple was investigated. all inducers were applied as foliar sprays to 1-mon-old pineapple plants (20 ml/plant) grown in 22-cm-diam. pots in the greenho ...	2006	19259535
oral immunogenicity of the plant proteinase bromelain.	bromelain is a natural mixture of proteolytic enzymes derived from pineapple stem that has been shown to have anti-inflammatory activity when administered orally. although most proteins given orally without adjuvant (e.g., food) result in tolerance, we previously reported that long-term oral exposure to bromelain stimulated the development of high serum anti-bromelain antibody titers. the purpose of these studies was to further investigate the mechanisms responsible for the immunogenicity of ora ...	2006	17161360
moulds and yeasts in fruit salads and fruit juices.	thirty-eight fruit salad samples including cantaloupe, citrus fruits, honeydew, pineapple, cut strawberries and mixed fruit salads, and 65 pasteurized fruit juice samples (apple, carrot, grapefruit, grape and orange juices, apple cider, and soy milk) were purchased from local supermarkets in the washington, dc area and tested for fungal contamination. the majority of fruit salad samples (97%) were contaminated with yeasts at levels ranging from <2.0 to 9.72 log10 of colony forming units per gram ...	2006	16943069
pineapple [ananas comosus (l.) merr].	a procedure for pineapple [ananas comosus (l.) merr.] genetic transformation is described, which involves temporary immersion bioreactors (tib) for selection of transgenic plants. success in the production of transgenic pineapple plants combines tissue culture factors. firstly, the use of regenerable pineapple callus as starting material for transformation whose cells shown to be competent for agrobacterium infection. secondly, the used of filtered callus, resulting in homogeneously sized cluste ...	2006	17033065
pharmacokinetic study of p-coumaric acid in mouse after oral administration of extract of ananas comosus l. leaves.	quantification of p-coumaric acid in mouse plasma following oral administration of ananas comosus l. leaves was achieved by reversed-phase high-performance liquid chromatography using a mobile phase of water-acetonitrile (82:18, v/v) and uv detection at 310 nm. the method was linear (determination coefficient, r2 = 0.9997) within the tested range (0.04-1.28 microg/ml). intra- and inter-day precision coefficients of variation and accuracy bias were acceptable (maximal cv value was 4.06% for intra ...	2006	16506268
production of natural fruity aroma by geotrichum candidum.	based on its aromatic potential, geotrichum candidum isolated from olive vegetation water was tested for the production of volatile compounds. when g. candidum was cultivated on media with glucose as the carbon source, flavor volatile compounds were produced and accumulated in the broth. fruity flavoring compounds (pineapple-like) such as esters and alcohols were analyzed by gas chromatography coupled to mass spectrometry, including ethyl esters of acetic acid and butyric acid, methyl-3-butan-1- ...	2006	16632883
the effect of preservatives on the sterility of microorganisms introduced into different fruit juices.	the aim of this study was to investigate the degree of sterility of some fruit juices post manufacture and to determine how effective the preservatives in them aid the killing of some microorganisms introduced into them.	2006	16641872
in vitro screening of six anthelmintic plant products against larval haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay.	because of the increasing anthelmintic resistance and the impact of conventional anthelmintics on the environment, it is important to look for alternative strategies against gastrointestinal nematodes. phytotherapy could be one of the major options to control these pathologies. extracts or ingredients of six different plant species were tested against exsheathed infective larvae of haemonchus contortus using a modified methyl-thiazolyl-tetrazolium (mtt) reduction assay. pyrantel tartrate was use ...	2006	16725288
effect of ethanolic extracts of ananas comosus l. leaves on insulin sensitivity in rats and hepg2.	ethanolic extracts of ananas comosus l. leaves (ac) enriched with phenols have hypoglycemic activity in diabetic rats. here, we investigated the effect of ac on insulin sensitivity in rats and hepg2. in high-fat diet-fed and low-dose streptozotozin-treated diabetic wistar rats subjected to challenge with exogenous human insulin, ac treatment at an oral dose of 0.40 g/kg could significantly improve sensitivity to exogenous insulin. after a sub-acute treatment, ac also could inhibit the developmen ...	2006	16753349
application of the broad-spectrum bacteriocin enterocin as-48 to inhibit bacillus coagulans in canned fruit and vegetable foods.	the enterococcal bacteriocin (enterocin) as-48 is a broad-spectrum cyclic peptide. enterocin as-48 was tested against bacillus coagulans in three vegetable canned foods: tomato paste (ph 4.64), syrup from canned peaches (ph 3.97), and juice from canned pineapple (ph 3.65). when vegetative cells of b. coagulans cect (spanish type culture collection) 12 were inoculated in tomato paste supplemented with 6 microg/ml as-48 and stored at different temperatures, viable cell counts were reduced by appro ...	2006	16831501
in vitro and in vivo anthelmintic efficacy of plant cysteine proteinases against the rodent gastrointestinal nematode, trichuris muris.	extracts of plants, such as papaya, pineapple and fig, are known to be effective at killing intestinal nematodes that inhabit anterior sites in the small intestine, such as heligmosomoides polygyrus. in this paper, we demonstrate that similar in vitro efficacy also occurs against a rodent nematode of the large intestine, trichuris muris, and confirm that the cysteine proteinases present in the plant extracts are the active principles. the mechanism of action of these enzymes involved an attack o ...	2006	16448585
blackberry (rubus spp.): a ph-dependent oral contrast medium for gastrointestinal tract images by magnetic resonance imaging.	in this study, seven fruits have been tested on their magnetic properties, paramagnetic metal content and contrast enhancement in magnetic resonance imaging (mri) of phantom and in vivo. magnetic susceptibility was determined for the fruit pulps, as well as the contents of paramagnetic metals; iron, manganese and copper. the total content of these metals was 4.3, 8.6, 11.1, 10.9, 12.3, 8.3 and 29.3 mg/kg of fruit for plum, blueberry, apple (red), pineapple, beet, grape, blackberry, respectively, ...	2006	16455409
bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.	ileus continues to be a common consequence of abdominal surgery, causing significant patient discomfort and often leading to more serious problems. the therapy available is limited, hence, ileus remains an important clinical problem. activation of inducible nitric oxide synthase (inos) directly modulates intestinal dysmotility after bowel manipulation and plays an essential role in initiating intestinal inflammation. nuclear factor (nf)-kappab is known to be a critical component of inos gene tra ...	2006	16137711
efficient production of transgenic citrus plants using isopentenyl transferase positive selection and removal of the marker gene by site-specific recombination.	the presence of marker genes conferring antibiotic resistance in transgenic plants represents a serious obstacle for their public acceptance and future commercialization. in citrus, selection using the selectable marker gene nptii, that confers resistance to the antibiotic kanamycin, is in general very effective. an attractive alternative is offered by the mat system (multi-auto-transformation), which combines the ipt gene for positive selection with the recombinase system r/rs for removal of ma ...	2007	16927091
'complementary ent': a systematic review of commonly used supplements.	to assess the evidence surrounding the use of certain complementary supplements in otolaryngology. we specifically focussed on four commonly used supplements: spirulina, ginkgo biloba, vertigoheel and nutritional supplements (cod liver oil, multivitamins and pineapple enzyme).	2007	17125579
anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, protospirura muricola, in vitro and in vivo.	cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, heligmosomoides polygyrus (which is found in the anterior small intestine) and trichuris muris (which resides in the caecum). proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. in this paper, we describe the in vitro and in vivo effect ...	2007	17032468
the growth of propionibacterium cyclohexanicum in fruit juices and its survival following elevated temperature treatments.	this study investigated the growth of propionibacterium cyclohexanicum in orange juice over a temperature range from 4 to 40 degrees c and its ability to multiply in tomato, grapefruit, apple, pineapple and cranberry juices at 30 and 35 degrees c. survival after 10 min exposure to 50, 60, 70, 80, 85, 90 and 95 degrees c in culture medium and in orange juice was also assessed. in orange juice the organism was able to multiply by 2 logs at temperatures from 4 to 35 degrees c and survived for up to ...	2007	17189756
a synthetic analog of plantaricin 149 inhibiting food-borne pathogenic bacteria:evidence for alpha-helical conformation involved in bacteria-membrane interaction.	plantaricin-149 is a bacteriocin produced by lactobacillus plantarum nric 149 (a lab isolated from pineapple), which consists of a peptidic chain made up of 22 amino acid residues [kato et al. j. ferment. bioeng. 1994; 77: 277-282]. in this work, a synthetic c-terminal amidated peptide analog denoted pln149a was prepared by spps-fmoc chemistry and the antagonistic activity against gram-positive and gram-negative bacteria was tested. the secondary structure was studied by circular dichroism (cd) ...	2007	17266050
prooxidant action of furanone compounds: implication of reactive oxygen species in the metal-dependent strand breaks and the formation of 8-hydroxy-2'-deoxyguanosine in dna.	prooxidant properties of furanone compounds including 2,5-furanone (furaneol, 4-hydroxy-2,5-dimethyl-furan-3-one), 4,5-furanone (4,5-dimethyl-3-hydroxy-2(5h)-furanone) (sotolone) and cyclotene (2-hydroxy-3-methyl-2-cyclopenten-1-one) were analyzed in relation to the metal-reducing activity. only 2.5-furanone known as a "strawberry or pineapple furanone" inactivated aconitase the most sensitive enzyme to active oxygen in the presence of ferrous sulfate, suggesting the furaneol/iron-mediated gener ...	2007	17316945
genetic component of identification, intensity and pleasantness of odours: a finnish family study.	although potential odorant receptor genes have been identified, the precise genetic component of perception of odours is still obscure. although there is some evidence for heritability of a few olfactory-related traits, no genome-wide search for loci harboring underlying genes has been published to date. we performed a genome-wide scan to identify loci affecting the identification, intensity and pleasantness of 12 odours (cinnamon, turpentine, lemon, smoke, chocolate, rose, paint thinner, banana ...	2007	17342154
biological detoxification of cr(vi) using wood-husk immobilized acinetobacter haemolyticus.	acinetobacter haemolyticus, a gram-negative aerobic locally isolated bacterium, immobilized on wood-husk showed the ability to detoxify cr(vi) to cr(iii). wood-husk, a natural cellulose-based support material, packed in an upward-flow column was used as support material for bacterial attachment. around 97% of the cr(vi) in wastewater containing 15 mg l(-1) of cr(vi) was reduced at a flow rate of 8.0 ml min(-1). the wastewater containing cr(vi) was added with liquid pineapple wastewater as nutrie ...	2007	17368716
hypolipidemic mechanisms of ananas comosus l. leaves in mice: different from fibrates but similar to statins.	in this study, we investigated hypolipidemic mechanisms of the ethanolic extract of ananas comosus l. leaves (ac) in mice and then determined its activities in related enzymes. the results showed that ac (0.40 g/kg) significantly inhibited the increase in serum triglycerides by 40% in fructose-fed mice. in mice induced by alloxan and high-fat diets, serum total cholesterol remained at a high level (180 - 220 mg/dl) within 7 days of removing high-fat diets but reached normal level (120 - 140 mg/d ...	2007	17380035
production of butyl acetate ester by lipase from novel strain of rhizopus oryzae.	a new lipase preparation from rhizopus oryzae was used to catalyze the esterification reaction between acetic acid and butanol to produce butyl acetate ester (pineapple flavor). this flavor compound can be used in food, cosmetic and pharmaceutical industries. only 3% of butyl acetate was obtained when free lipase was used in the synthesis containing only the substrates. in contrast, the conversion yield reached 25% when immobilized lipase was used under the same conditions. the synthesis of buty ...	2007	17502279
detection of some safe plant-derived foods for ltp-allergic patients.	lipid transfer protein (ltp) is a widely cross-reacting plant pan-allergen. adverse reactions to rosaceae, tree nuts, peanut, beer, maize, mustard, asparagus, grapes, mulberry, cabbage, dates, orange, fig, kiwi, lupine, fennel, celery, tomato, eggplant, lettuce, chestnut and pineapple have been recorded.	2007	17505138
effectiveness of radiation processing for elimination of salmonella typhimurium from minimally processed pineapple (ananas comosus merr.).	the microbiological quality of market samples of minimally processed (mp) pineapple was examined. the effectiveness of radiation treatment in eliminating salmonella typhimurium from laboratory inoculated ready-to-eat pineapple slices was also studied. microbiological quality of minimally processed pineapple samples from mumbai market was poor; 8.8% of the samples were positive for salmonella. d(10) (the radiation dose required to reduce bacterial population by 90%) value for s. typhimurium inocu ...	2007	17995808
inhibitory effect of fruit extracts on the formation of heterocyclic amines.	natural extracts have attracted considerable attention for development into effective inhibitors against the formation of genotoxic heterocyclic amines (has) in processed foods. in this study, four fruit extracts (apple, elderberry, grape seed, and pineapple) were evaluated for their effects on ha formation in fried beef patties. apple and grape seed extracts were found to be the most effective in both the degree of inhibition in the formation of individual has (2-amino-3,8-dimethylimidazo [4,5- ...	2007	18004801
in vitro anthelmintic effects of cysteine proteinases from plants against intestinal helminths of rodents.	infections with gastrointestinal (gi) nematodes are amongst the most prevalent worldwide, especially in tropical climates. control of these infections is primarily through treatment with anthelmintic drugs, but the rapid development of resistance to all the currently available classes of anthelmintic means that alternative treatments are urgently required. cysteine proteinases from plants such as papaya, pineapple and fig are known to be substantially effective against three rodent gi nematodes, ...	2007	18005461
microbial control of arthropod pests of tropical tree fruits.	a multitude of insects and mites attack fruit crops throughout the tropics. the traditional method for controlling most of these pests is the application of chemical pesticides. growing concern on the negative environmental effects has encouraged the development of alternatives. inundatively and inoculatively applied microbial control agents (virus, bacteria, fungi, and entomopathogenic nematodes) have been developed as alternative control methods of a wide variety of arthropods including tropic ...	2007	17607448
functional characterization of enone oxidoreductases from strawberry and tomato fruit.	fragaria x ananassa enone oxidoreductase (faeo), earlier putatively assigned as quinone oxidoreductase, is a ripening-induced, negatively auxin-regulated enzyme that catalyzes the formation of 4-hydroxy-2,5-dimethyl-3(2h)-furanone (hdmf), the key flavor compound in strawberry fruit by the reduction of the alpha,beta-unsaturated bond of the highly reactive precursor 4-hydroxy-5-methyl-2-methylene-3(2h)-furanone (hmmf). here we show that recombinant faeo does not reduce the double bond of straight ...	2007	17636940
nematicidal effects of cysteine proteinases against sedentary plant parasitic nematodes.	cysteine proteinases from the fruit and latex of plants, such as papaya, pineapple and fig, have previously been shown to have substantial anthelmintic efficacy, in vitro and in vivo, against a range of animal parasitic nematodes. in this paper, we describe the in vitro effects of these plant extracts against 2 sedentary plant parasitic nematodes of the genera meloidogyne and globodera. all the plant extracts examined caused digestion of the cuticle and decreased the activity of the tested nemat ...	2007	17640402
in vivo antitumoral activity of stem pineapple (ananas comosus) bromelain.	stem bromelain (ec 3.4.22.32) is a major cysteine proteinase, isolated from pineapple ( ananas comosus) stem. its main medicinal use is recognized as digestive, in vaccine formulation, antitumoral and skin debrider for the treatment of burns. to verify the identity of the principle in stem fractions responsible for the antitumoral effect, we isolated bromelain to probe its pharmacological effects. the isolated bromelain was obtained from stems of adult pineapple plants by buffered aqueous extrac ...	2007	17893836
carotenoids and color of fruit juice and milk beverage mixtures.	seventeen commercially available, "ready to drink" fortified beverages consisting of mixtures of fruit juices and milk were analyzed to evaluate their carotenoid profile (including their z/e stereoisomers) and color during their commercial shelf life. lightness (l*) was found to be correlated with the content of milk in the mixtures (r= 0.649) whereas red-yellow colors were correlated with the contents of alpha-carotene, beta-cryptoxanthin, and beta-carotene supplied by the fruit. the beverages ...	2007	18034704
heavy metals removal from contaminated sewage sludge by naturally fermented raw liquid from pineapple wastes.	the large amount of unutilised pineapple wastes produced every year in tropical countries, particularly in thailand, adds to the existing environmental pollution problems of the country. this study investigated the utilisation of pineapple wastes to treat another form of waste (sludge) from wastewater treatment facilities in thailand. laboratory scale studies were carried out to determine the potential of using naturally fermented raw liquid from pineapple wastes as a source of citric acid in th ...	2007	17951878
bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems.	livestock and aquaculture production is under political and social pressure, especially in the european union (eu), to decrease pollution and environmental damage arising due to animal agriculture. the eu has banned the use of antibiotics and other chemicals, which have been shown to be effective in promoting growth and reducing environment pollutants because of the risk caused to humans by chemical residues in food and by antibiotic resistance being passed on to human pathogens. as a result of ...	2007	22444893
characterization of active phenolic components in the ethanolic extract of ananas comosus l. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometry.	hplc-dad-ms was utilized to investigate the phytochemical constituents in ethanolic extract of ananas comosus l. leaves (eeacl) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on hplc retention time, uv and m ...	2007	17698075
role of bromelain in the treatment of patients with pityriasis lichenoides chronica.	pityriasis lichenoides chronica (plc) is a skin disease of unknown etiology. uncertainty about the etiopathogenesis of this skin disease is the reason for the unpredictable and non optimal efficacy of therapies available for its treatment. the aim of the present study was to evaluate the efficacy of bromelain, a crude aqueous extract of the stems and immature fruit of pineapple, in the treatment of plc.	2007	17671882
determination of neutral carbohydrates by cze with direct uv detection.	a new cze method relying on in-capillary reaction and direct uv detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, d-(-)-mannitol, sucrose, d-(+)-fucose, d-(+)-cellobiose, d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(-)-arabinose, d-(+)-xylose, and d-(-)-ribose. the alkaline electrolyte solution was prepared of 130 mm sodium hydroxide and 36 mm disodium hydrogen phosphate dihydrate. separation of the sample mixtu ...	2007	17661315
[determination of 1, 3-o-dicaffeoylglycerides from leaves of pineapple by hplc].	to establish a hplc method for a new compound 1,3-o-dicaffeoylglycerides determination.	2007	17639981
enhanced separation of membranes during free flow zonal electrophoresis in plants.	free flow zonal electrophoresis (ffze) is a versatile technique that allows for the separation of cells, organelles, membranes, and proteins based on net surface charge during laminar flow through a thin aqueous layer. we have been optimizing the ffze technique to enhance separation of plant vacuolar membranes (tonoplast) from other endomembranes to pursue a directed proteomics approach to identify novel tonoplast transporters. addition of atp to a mixture of endomembranes selectively enhanced e ...	2007	17566980
cytokinins and auxin communicate nitrogen availability as long-distance signal molecules in pineapple (ananas comosus).	this work aimed at identifying a possible role of phytohormones in long-distance (root-shoot) signaling under nitrogen deficiency. three-months old pineapple plants were transferred from murashige and skoog (ms) medium to nitrogen-free ms (-n). during the first 24h on -n, 20 plants were harvested every 4h. after 30 days in -n, the remaining plants were transferred back to regular ms (+n) and 20 plants harvested every 4h for the first 24h. following the harvests, endogenous levels of nitrate (no( ...	2007	17566606
design and development of batch type acetifier for wine-vinegar production.	a batch type acetifier based on the principal of acetic acid fermentation was designed and tested for production of wine-vinegar from the pineapple peel waste. the pineapple peels along with starter solution was fed to the inner ss perforated peel-solid separator tank 130 mm dia having perforations of 50 mm size. the concentric perforated peel-solid separator circular tank was fitted inside the collecting tank having 255 mm dia. the pineapple peels and starter solution in perforated peel-solid s ...	2007	23100658
[new cerebroside from leaves of pineapple].	to study the chemical constituents of the leaves of pineapple.	2007	17511144
pineapple juice and its fractions in enzymatic browning inhibition of banana [musa (aaa group) gros michel].	the effectiveness of pineapple juice in enzymatic browning inhibition was evaluated on the cut surface of banana slices. after storage of banana slices at 15 degrees c for 3 days, pineapple juice showed browning inhibition to a similar extent as 8 mm ascorbic acid but less than 4 mm sodium metabisulfite. fractionation of pineapple juice by a solid-phase c18 cartridge revealed that the directly eluted fraction (de fraction) inhibited banana polyphenol oxidase (ppo) about 100% when compared to the ...	2007	17439237
disease regression in malignant melanoma: spontaneous resolution or a result of treatment with antioxidants, green tea, and pineapple cores? a case report.		2007	17393612
[occupational allergies to bromelain].	the protease bromelain originating from the pineapple fruit (ananas comosus) finds frequent use in industry. exposure to enzyme dusts has long been known to cause occupational allergies. the present paper reviews the results of the evaluation of literature data concerning occupational airway sensitization due to bromelain. cases of specific airway sensitization caused by bromelain could be shown clearly by the presented studies. since the symptoms, results of skin prick tests, detection of speci ...	2007	17342576
[pineapple juice as a negative oral contrast agent in magnetic resonance cholangiopancreatography].	the quality of magnetic resonance cholangiopancreatography (mrcp) images is frequently degraded by high signal from the gastrointestinal tract on heavily t2w images. the purpose of this study is to evaluate pineapple juice (pj) as an oral negative contrast agent in mrcp.	2007	18065928
a comparison of nutrient density scores for 100% fruit juices.	the 2005 dietary guidelines for americans recommend that consumers choose a variety of nutrient-dense foods. nutrient density is usually defined as the quantity of nutrients per calorie. food and nutrition professionals should be aware of the concept of nutrient density, how it might be quantified, and its potential application in food labeling and dietary guidance. this article presents the concept of a nutrient density score and compares nutrient density scores for various 100% fruit juices. o ...	2007	17995788
quantification of selected aroma-active compounds in strawberries by headspace solid-phase microextraction gas chromatography and correlation with sensory descriptive analysis.	selected aroma-active compounds in strawberries were quantified using headspace solid-phase microextraction and gas chromatography. ten strawberry cultivars grown in california and oregon were studied. the standard curves were built in a synthetic matrix and quantification was achieved using multiple internal standards. odor activity values (oavs) of the aroma compounds were calculated to understand their contribution to the overall aroma. although the concentrations of the aroma compounds varie ...	2007	17995662
[role of green tea in oxidative stress prevention].	oxidative stress is a condition caused by an increase of reactive oxygen species (ros) or by a shortage of the mechanisms of cellular protection and antioxidant defence. ros have a potential oxidative effect towards various cellular macromolecules: proteins, nucleic acids, proteoglycans, lipids, with consequent damages in several cellular districts and promotion of the ageing process of the organism. however, some substances are able to prevent and/or reduce the damages caused by ros; therefore, ...	2007	17228849
improved detection of sugar addition to maple syrup using malic acid as internal standard and in 13c isotope ratio mass spectrometry (irms).	stable carbon isotope ratio mass spectrometry (delta13c irms) was used to detect maple syrup adulteration by exogenous sugar addition (beet and cane sugar). malic acid present in maple syrup is proposed as an isotopic internal standard to improve actual adulteration detection levels. a lead precipitation method has been modified to isolate quantitatively malic acid from maple syrup using preparative reversed-phase liquid chromatography. the stable carbon isotopic ratio of malic acid isolated fro ...	2007	17227042
sensory-specific satiety with simple foods in humans: no influence of bmi?	olfacto-gustatory sensory-specific satiety plays an important role in the termination of food ingestion. a defect in this mechanism, by increasing food intake, could be a factor in development of overweight. the present study was conducted to explore whether sensory-specific satiety in the overweight may be different from that in normal-weight subjects.	2007	17160089
multiresidue method for fast determination of pesticides in fruit juices by ultra performance liquid chromatography coupled to tandem mass spectrometry.	a new analytical method for the simultaneous determination of 90 pesticides in fruit juices by ultra performance liquid chromatography coupled to tandem mass spectrometry (uplc-ms/ms) has been developed and validated. extraction was performed with acetonitrile, applying quechers methodology, and the extracts were analyzed without any further clean-up step, providing better results than solid phase extraction (spe) procedure. before chromatographic step, extracts were diluted with water (1:1) in ...	2008	18585265
assessment of citrate concentrations in citrus fruit-based juices and beverages: implications for management of hypocitraturic nephrolithiasis.	dietary intake of citrate in the form of citrus juices (eg, lemonade, orange juice) will enhance urinary citrate excretion, a valuable benefit for patients with hypocitraturic calcium oxalate nephrolithiasis. while information on citrate concentrations in select citrus juices is available, data on citrate concentrations of commercially available beverages (juice and otherwise) are limited. using nuclear magnetic resonance spectroscopy (1h nmr), we report citrate concentrations of several beverag ...	2008	18578663
use of reverse micellar systems for the extraction and purification of bromelain from pineapple wastes.	reverse micellar systems of ctab/isooctane/hexanol/butanol and aot/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). the effect of forward as well as back extraction process parameters on the extraction efficiency, activity recovery and purification fold is studied in detail for the pineapple core extract. the optimized conditions for the extraction from core resulted in forward and ba ...	2008	17964777
multi-walled carbon nanotubes as efficient solid-phase extraction materials of organophosphorus pesticides from apple, grape, orange and pineapple fruit juices.	multi-walled carbon nanotubes (mwcnts) have been used for the first time as solid-phase extraction (spe) sorbents for the extraction of eight organophosphorus pesticides (i.e. ethoprophos, diazinon, chlorpyriphos-methyl, fenitrothion, malathion, chlorpyriphos, fenamiphos and buprofezin) from different commercial fruit juices (i.e. apple, grape, orange and pineapple). the developed method, which involves spe and direct gas chromatography with nitrogen phosphorus detection analysis, is very fast, ...	2008	18849040
the effect of different hormones and incubation periods on in vitro proliferation of pineapple (ananas comosus l.) merr cv. smooth cayenne) shoot-tip culture.	seven different hormone treatments, namely 6-benzylaminopurine (bap) at 2, 3 mg l(-1) was applied singly and in combination with indole acetic acid (iaa) at 0.18, 0.8 and 1.8 mg l(-l), bap at 3.3 mg l(-l) in combination with iaa at 1.8 and 3.3 mg l(-l) and triple combination of bap at 2.3, iaa at 1.8 and gibberellic acid (ga3) at 1.0 mg l(-1) were tested, over four different incubation periods of 30, 45, 60 and 75 days, for their effect in the proliferation and growth of smooth cayenne pineapple ...	2008	18817160
preparation of ferulic acid from agricultural wastes: its improved extraction and purification.	ferulic acid (fa) is a phenolic antioxidant present in plants, which is widely used in the food and cosmetic industry. in the present study, various agricultural wastes such as maize bran, rice bran, wheat bran, wheat straw, sugar cane baggasse, pineapple peels, orange peels, and pomegranate peels were screened for the presence of esterified fa (efa). among the sources screened, maize bran was found to contain the highest amount of efa. pineapple peels, orange peels, and pomegranate peels were a ...	2008	18707110
crystal structures of saturn-like c50cl10 and pineapple-shaped c64cl4: geometric implications of double- and triple-pentagon-fused chlorofullerenes.		2008	18548468
ultrasound as pre-treatment for drying of pineapple.	dehydration of fruits is an alternative to reduce post-harvest loss of fruits and also a process to produce dried fruits, which can be directly consumed or become part of foodstuffs like cakes, pastries and many others. the effect of ultrasonic pre-treatment and ultrasound-assisted osmotic dehydration, before air-drying, on dehydration of pineapple (ananas comosus) was investigated. this study allowed estimating the water diffusivity in the air-drying process for pineapples submitted to ultrasou ...	2008	18462985
malate metabolism in hoya carnosa mitochondria and its role in photosynthesis during cam phase iii.	this study investigated the respiratory properties and the role of the mitochondria isolated from one phosphoenolpyruvate carboxykinase (pck)-cam plant, hoya carnosa, in malate metabolism during cam phase iii. the mitochondria showed high malate dehydrogenase (mmdh) and aspartate amino transferase (mast), and a significant amount of malic enzyme (mme) activities. h. carnosa readily oxidized malate via mme and mmdh in the presence of some cofactors such as thiamine pyrophosphate (tpp), coenzyme a ...	2008	18403382
movement of bromacil in a hawaii soil under pineapple cultivation - a field study.	recent discovery of low concentrations of bromacil in drinking water prompted the state of hawaii to examine the leaching behavior of bromacil in pineapple fields. this study is a follow up to earlier work on bromacil concentrations in soil profiles in a pineapple field in central oahu, hawaii. soil samples were collected for bromacil analysis at different times prior to and after application from a pineapple field that was previously surveyed by other research workers. the leaching pattern of b ...	2008	18374966
glycemic index of common malaysian fruits.	the objective of the present study was to measure the glycemic index of durian, papaya, pineapple and water-melon grown in malaysia. ten (10) healthy volunteers (5 females, 5 males; body mass index 21.18+/-1.7 kg/m2) consumed 50 g of available carbohydrate portions of glucose (reference food) and four test foods (durian, papaya, pineapple and watermelon) in random order after an overnight fast. glucose was tested on three separate occasions, and the test foods were each tested once. postprandial ...	2008	18364324
effect of ionizing radiation on furan formation in fresh-cut fruits and vegetables.	furan, a possible carcinogen, is commonly induced by thermal processing in a wide variety of foods. the possible formation of furan from fresh-cut fruits and vegetables due to irradiation was studied. nineteen fresh-cut fruits and vegetables were irradiated by 5 kgy gamma rays at 4 degrees c. furan was analyzed from the irradiated samples using a number of preparation methods. the results showed that almost all tested fruits and vegetables, upon irradiation, produced nondetectable levels, or les ...	2008	18298720
enzymatic necrolysis of acute deep burns--report of preliminary results with 22 patients.	enzymatic debridement by the use of a proteolytic enzyme complex derived, isolated and purified from pineapple stems proves to be an innovative, rapid, effective, selective and safe method of postburn necrotic skin removal. the major advantages of the procedure include minimal invasivity, rapidity, effectiveness, possibility to perform the debridement at the bedside, minimal or no loss of blood and minimal interference with natural wound healing processes. our preliminary experience with this tr ...	2008	19408486
perceptual processing strategy and exposure influence the perception of odor mixtures.	in flavor perception, both experience with the components of odor/taste mixtures and the cognitive strategy used to examine the interactions between the components influence the overall mixture perception. however, the effect of these factors on odor mixtures perception has never been studied. the present study aimed at evaluating whether 1) previous exposure to the odorants included in a mixture or 2) the synthetic or analytic strategy engaged during odorants mixture evaluation determines odor ...	2008	18071197
multiresidue method for the determination of residues of 251 pesticides in fruits and vegetables by gas chromatography/mass spectrometry and liquid chromatography with fluorescence detection.	a method is described for the determination of 251 pesticide and degradation product residues in fruit and vegetable samples. extraction of the sample with acetonitrile is followed by a salting-out step. co-extractives are removed by passing a portion of the acetonitrile extract through an octadecyl (c18) solid-phase extraction cleanup cartridge and then, in a second cleanup, through a carbon cartridge coupled to an amino propyl cartridge. determination is by gas chromatography with mass-selecti ...	2008	10868594
evaluation of genetic variability in micropropagated propagules of ornamental pineapple [ananas comosus var. bracteatus (lindley) coppens and leal] using rapd markers.	the objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. the basal culture medium consisted of ms salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (bap) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/l. the addition of bap influenced the occurrence of genetic variation revealed using random amplified polymorphic dna (rapd) markers. of a tota ...	2008	19048488
biophenols from agro-industrial wastes.	large quantities of agro-based liquid wastes are produced every year and their disposal is often a problem for industries. in light of that, in this study prudent effort was done to screen the agro-industrial wastes - pineapple waste (paw) and palm oil mill effluent (pome) for valuable biophenols product. three different solvents; ethanol, acetone and distilled water were screened in order to enhance the process. all experiments were performed using fixed process conditions of solid to solvent r ...	2008	19025008
absolute side-chain structure at position 13 is required for the inhibitory activity of bromein.	bromelain isoinhibitor (bromein), a cysteine proteinase inhibitor from pineapple stem, has a unique double-chain structure. the bromein precursor protein includes three homologous inhibitor domains, each containing an interchain peptide between the light and heavy chains. the interchain peptide in the single-chain precursor is immediately processed by bromelain, a target proteinase. in the present study, to clarify the essential inhibitory site of bromein, we constructed 44 kinds of site-directe ...	2008	18948264
the importance and provision of oral hygiene in surgical patients.	the provision of mouth care on the general surgical ward and intensive care setting has recently gained momentum as an important aspect of patient care. oropharyngeal morbidity can cause pain and disordered swallowing leading to reluctance in commencing or maintaining an adequate dietary intake. on the intensive care unit, aside from patient discomfort and general well-being, oral hygiene is integral to the prevention of ventilator-associated pneumonia. chlorhexidine (0.2%) is widely used to dec ...	2008	18947816
antimicrobial activity and potential use of monoterpenes as tropical fruits preservatives.	banana, papaya and pineapple are the most consumed tropical fruits in the world, being brazil one of the main producers. fungi colletotrichum musae, colletotrichum gloeosporioides and fusarium subglutinans f.sp. ananas cause severe post harvest diseases and losses in fruits quality. the aim of this work was to evaluate the effectiveness of five monoterpenes to inhibit the mycelial growth and conidia germination of these three phytopathogens. the monoterpenes citral, citronellal, l-carvone, isopu ...	2008	24031197
application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste.	statistical experimental designs were applied for the optimization of medium constituents for citric acid production by yarrowia lipolytica ncim 3589 in solid state fermentation (ssf) using pineapple waste as the sole substrate. using plackett-burman design, yeast extract, moisture content of the substrate, kh(2)po(4) and na(2)hpo(4) were identified as significant variables which highly influenced citric acid production and these variables were subsequently optimized using a central composite de ...	2008	17936623
carotenoid production by xanthophyllomyces dendrorhous: use of pineapple juice as a production medium.	to select a carotenoid-hyperproducing mutant and to formulate pineapple juice as a carotenoid production medium.	2008	18673431
identification of viral and non-viral reverse transcribing elements in pineapple (ananas comosus), including members of two new badnavirus species.	a previously published partial sequence of pineapple bacilliform virus was shown to be from a retrotransposon (family metaviridae) and not from a badnavirus as previously thought. two newly discovered sequence groups isolated from pineapple were associated with bacilliform virions and were transmitted by mealybugs. phylogenetic analyses indicated that they were members of new badnavirus species. a third caulimovirid sequence was also amplified from pineapple, but available evidence suggests that ...	2008	18607672
inactivation of escherichia coli in a tropical fruit smoothie by a combination of heat and pulsed electric fields.	moderate heat in combination with pulsed electric fields (pef) was investigated as a potential alternative to thermal pasteurization of a tropical fruit smoothie based on pineapple, banana, and coconut milk, inoculated with escherichia coli k12. the smoothie was heated from 25 degrees c to either 45 or 55 degrees c over 60 s and subsequently cooled to 10 degrees c. pef was applied at electric field strengths of 24 and 34 kv/cm with specific energy inputs of 350, 500, and 650 kj/l. both processin ...	2008	19019120
pomegranate juice inhibits sulfoconjugation in caco-2 human colon carcinoma cells.	several fruit juices have been reported to cause food-drug interactions, mainly affecting cytochrome p450 activity; however, little is known about the effects of fruit juices on conjugation reactions. among several fruit juices tested (apple, peach, orange, pineapple, grapefruit, and pomegranate), pomegranate juice potently inhibited the sulfoconjugation of 1-naphthol in caco-2 cells. this inhibition was both dose- and culture time-dependent, with a 50% inhibitory concentration (ic(50)) value ca ...	2008	19053852
the role of enzyme supplementation in digestive disorders.	this article reviews various forms of enzyme supplementation used clinically in digestive and absorption disorders. enzyme supplementation plays an integral role in the management of various digestive disorders, particularly with regard to exocrine pancreatic insufficiency. however, application of enzymes may also be beneficial for other conditions associated with poor digestion including lactose intolerance. historically, porcine and bovine pancreatic enzymes have been the preferred form of sup ...	2008	19152478