Publications

characterization of acylated anthocyanins in callus induced from storage root of purple-fleshed sweet potato, ipomoea batatas l.	four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (ipomoea batatas l) cultivar ayamurasaki. the anthocyanins were respectively identified as cyanidin 3- $o$ -(2- $o$ -(6- $o$ -( $e$ )-caffeoyl- $\beta$ -d-glucopyranosyl)- $\beta$ -d-glucopyranoside)-5- $o$ - $\beta$ -d-glucopyranoside, cyanidin 3- $o$ -(2- $o$ -(6- $o$ -( $e$ )- $p$ -coumaroyl- $\beta$ -d-glucopyranosyl)-6- $o$ -( $e$ )-caffeoyl- $\beta$ -d-glucopyranoside ...	2004	15577190
alterations in intracellular and extracellular activities of antioxidant enzymes during suspension culture of sweetpotato.	cultured plant cells are a good system for the study of antioxidant mechanisms and for the mass production of antioxidants, because they can be grown under conditions of high oxidative stress. alterations in the intracellular and extracellular activities of three antioxidant enzymes, superoxide dismutase (sod), guaiacol-type peroxidase (pod), and glutathione peroxidase (gpx), were investigated in suspension cultures of sweetpotato (ipomoea batatas) during cell growth. intracellular sod activitie ...	2004	15381411
birthweight and environment at tari.	the weights at birth of 4767 children born in the tari area between 1979 and 1986 were analyzed after the children had been allocated to seven environmental zones. the environments differ in the quality of land from which the staple food, sweet potato (ipomoea batatas), is produced. mean birthweight varies significantly by environment. women do much of the day-to-day agricultural work. it is argued that women living in the poorer quality environments produce less food, suffer chronic malnourishm ...	2004	14658831
phylogenetic relationships of the sweetpotato in ipomoea series batatas (convolvulaceae) based on nuclear beta-amylase gene sequences.	phylogenetic relationships of 13 accessions and a cultivar representing the sweetpotato, ipomoea batatas (l.) lam., and its wild progenitors, were investigated using the nucleotide sequence variation of a nuclear-encoded beta-amylase gene. a 1.1-1.3 kb fragment of the gene spanning two exons separated by a long intron was pcr-amplified, cloned, and sequenced. exon sequences proved highly conservative, while intron sequences yielded large differences. intron analyses grouped species in a phylogen ...	2004	15012943
efficacy of ipomoea batatas (caiapo) on diabetes control in type 2 diabetic subjects treated with diet.	to investigate the tolerability, efficacy, and mode of action of caiapo, an extract of white sweet potatoes, on metabolic control in type 2 diabetic patients.	2004	14747225
rapid and reliable method of extracting dna and rna from sweetpotato, ipomoea batatas (l). lam.	a quick, simple and reliable method of extracting dna from sweetpotato (ipomoea batatas (l.) lam.) has been developed. the method was applied successfully for extraction of total dna from leaves and total rna from leaves and various tissues. the yield of dna extracted by this procedure was high (about 1 mg/g leaf tissue). the extracted dna was completely digested by restriction endonucleases indicating the absence of common contaminating compounds. the absorbancy ratios of a260/a230 and a260/a28 ...	2005	16328977
the regulation of rubisco activity in response to variation in temperature and atmospheric co2 partial pressure in sweet potato.	the temperature response of net co(2) assimilation rate (a), the rate of whole-chain electron transport, the activity and activation state of rubisco, and the pool sizes of ribulose-1,5-bisphosphate (rubp) and 3-phosphoglyceric acid (pga) were assessed in sweet potato (ipomoea batatas) grown under greenhouse conditions. above the thermal optimum of photosynthesis, the activation state of rubisco declined with increasing temperature. doubling co(2) above 370 mubar further reduced the activation s ...	2005	16183840
probing anthocyanin profiles in purple sweet potato cell line (ipomoea batatas l. cv. ayamurasaki) by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry.	a purple line cell line (pl) generated from the storage root of purple-fleshed sweet potato (ipomoea batatas l.) cv. ayamurasaki produces a complex mixture of anthocyanins, and seven major anthocyanins have been isolated and identified to date. all these anthocyanins are exclusively cyanidin or peonidin 3-sophoroside-5-glucosides and their acylated derivatives. high-performance liquid chromatography (hplc) coupled to photodiode array (pda) detection and electrospray ionization tandem mass spectr ...	2005	16076141
[study on flavonoids from leaf of ipomoea batatas].	to investigate the flavonoid constituents of ipomoea batatas.	2005	16011096
estrogenic effect of yam ingestion in healthy postmenopausal women.	yam (dioscorea) has been used to treat menopausal symptom folklorically. this study was to investigate the effects of yam ingestion on lipids, antioxidant status, and sex hormones in postmenopausal women.	2005	16093400
risk factors for kidney cancer in a japanese population: findings from the jacc study.	the incidence of kidney cancer is high in western and northern europe and north america, and low in asia. although the incidence of kidney cancer in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing.	2005	16127235
identification of the molting hormone of the sweet potato (bemisia tabaci) and greenhouse (trialeurodes vaporariorum) whitefly.	in order to identify the whitefly molting hormone, whole body extracts of mature 4th instar and newly formed pharate adult bemisia tabaci (biotype b) and trialeurodes vaporariorum were prepared and subjected to reverse phase high performance liquid chromatography (rphplc). ecdysteroid content of fractions was determined by enzymeimmunoassay (eia). the only detectable ecdysteroids that were present in significant amounts in whitefly extracts were ecdysone and 20-hydroxyecdysone. the concentration ...	2005	15686645
host-parasitoid interactions relating to penetration of the whitefly, bemisia tabaci, by the parasitoid wasp, eretmocerus mundus.	it has been reported that the aphelinid wasp eertmocerus mundus parasitizes all four nymphal instars of the sweet potato whitefly, bemisia tabaci (biotype b), with 3rd instars being the preferred hosts. the parasitoid lays its egg on the leaf underneath the host nymph. first instars hatch and later penetrate the whitefly. previous studies have shown that the initiation of parasitoid penetration induces the host to form a cellular capsule around the parasitoid. as described here, females never ov ...	2005	17119628
[field distribution pattern and metabolic activity of am fungi and their effects on ipomoea batatas growth].	the study showed that after eight weeks of inoculating sweet potato seedlings with am fungus glomus intraradices beg141 under field condition, the shoot dry weight, individuals of tuber and their fresh weight, as well as the p uptake by the plant increased significantly. compared with the control, the root colonization rate, extraradical hyphal density and intraradical alkaline phosphatase (alp) activity were greatly increased. after inoculation with beg141, the total and succinate dehydrogenase ...	2005	15852976
two cis-acting regulatory elements are involved in the sucrose-inducible expression of the sporamin gene promoter from sweet potato in transgenic tobacco.	in this study, we generated transgenic tobacco plants that express the beta-glucuronidase (gus) gene under the control of the 305-bp 5'-upstream region of a gene coding for sporamin a of sweet potato. expression of gus in excised tobacco leaves was induced by sucrose, mimicking the sugar-inducible expression of the endogenous sporamin genes in sweet potato. deletion of the sequences extending from position -305 (relative to the transcription start site) to -283 and from -146 to -87 resulted in a ...	2005	15654621
a regional food-frequency questionnaire for the us mississippi delta.	to describe food sources of nutrient intake for white and african american adults in the lower mississippi delta (lmd), and their use in the development of a regional food-frequency questionnaire (ffq) based on an earlier version of the national cancer institute's health habits and history questionnaire.	2005	15705249
utilization of ensiled sweet potato (ipomoea batatas (l.) lam.) leaves as a protein supplement in diets for growing pigs.	four diets were formulated with protein from fishmeal (fm), groundnut cake (gc), ensiled sweet potato (ipomoea batatas l. (lam.)) leaves (sp) or ensiled sweet potato leaves with lysine (spl). in experiment 1, 24 crossbred (large white x mong cai) growing pigs were allocated randomly by sex into four groups of six pigs and given one of four diets. experiment 2 was conducted using 16 crossbred pigs (large white x mong cai) at four farms. on each farm, pigs were allocated to two experimental groups ...	2005	15729899
effects on immune response of antidiabetic ingredients from white-skinned sweet potato (ipomoea batatas l.).	the present report describes the effects of antidiabetic ingredients from white-skinned sweet potato (awssp) on the immune response of human cells.	2005	15797679
the x-ray structure of a hemipteran ecdysone receptor ligand-binding domain: comparison with a lepidopteran ecdysone receptor ligand-binding domain and implications for insecticide design.	the ecdysone receptor is a hormone-dependent transcription factor that plays a central role in regulating the expression of vast networks of genes during development and reproduction in the phylum arthropoda. the functional receptor is a heterodimer of the two nuclear receptor proteins ecdysone receptor (ecr) and ultraspiracle protein. the receptor is the target of the environmentally friendly bisacylhydrazine insecticides, which are effective against lepidoptera but not against hemiptera or sev ...	2005	15809296
effects of carbon and nitrogen sources, carbon-to-nitrogen ratio, and initial ph on the growth of nematophagous fungus pochonia chlamydosporia in liquid culture.	the effects of carbon and nitrogen sources, carbon-to-nitrogen ratio (c:n) and initial ph value on the growth and sporulation of the nematophagous fungus pochonia chlamydosporia in liquid culture were examined. among the 21 carbon sources and 15 nitrogen compounds tested, the optimal carbon and nitrogen sources for mycelial growth were sweet potato and l: -tyrosine, and for sporulation were sweet potato and casein peptone. a c:n ratio of 10:1 at ph 3.7 gave the maximum yield of conidia and a c:n ...	2005	15883723
nitrogen requirements and urea recycling in an omnivorous marsupial, the northern brown bandicoot isoodon macrourus.	the maintenance nitrogen (n) requirement of the northern brown bandicoot, isoodon macrourus, was determined by giving captive animals diets based on a commercial small carnivore mix and shredded sweet potato, with 20% sand included in the moist diet to simulate the soil ingested by bandicoots during natural foraging. linear regression of n balance on n intake yielded maintenance n requirements of 438 mg kg(-0.75) d(-1) on a dietary basis and 413 mg kg(-0.75) d(-1) on a truly digestible basis. th ...	2005	15887092
viral class 1 rnase iii involved in suppression of rna silencing.	double-stranded rna (dsrna)-specific endonucleases belonging to rnase iii classes 3 and 2 process dsrna precursors to small interfering rna (sirna) or microrna, respectively, thereby initiating and amplifying rna silencing-based antiviral defense and gene regulation in eukaryotic cells. however, we now provide evidence that a class 1 rnase iii is involved in suppression of rna silencing. the single-stranded rna genome of sweet potato chlorotic stunt virus (spcsv) encodes an rnase iii (rnase3) ho ...	2005	15890961
antioxidative activity of anthocyanins from purple sweet potato, ipomoera batatas cultivar ayamurasaki.	we evaluated the antioxidative activity of anthocyanins from an extract of the tuber of purple sweet potato (psp) (ipomoea batatas cultivar ayamurasaki). anthocyanins from psp showed stronger 1,1-diphenyl-2-picrylhydrazyl (dpph) radical-scavenging activity than anthocyanins from red cabbage, grape skin, elderberry, or purple corn, and eight major components of the anthocyanins from psp showed higher levels of activity than ascorbic acid. in psp anthocyanin-injected rats and psp beverage-administ ...	2005	15914919
biological activity and chemical composition of the essential oil from jamaican hyptis verticillata jacq.	the chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of hyptis verticillata jacq. was elucidated by a combination of gc and gc-ms analyses. the oil was dominated by the sesquiterpenoids cadina-4,10(15)-dien-3-one (15.1%) (1) and aromadendr-1(10)-en-9-one (squamulosone) (30.7%) (2). the oil exhibited chemosterilant activities against the cattle tick, boophilus microplus canest., and toxic action against adult cylas formicarius elegantulus summer, the m ...	2005	15941314
human tartrate-resistant acid phosphatase becomes an effective atpase upon proteolytic activation.	proteolytic cleavage in an exposed loop of human tartrate-resistant acid phosphatase (tracp) with trypsin leads to a significant increase in activity. at each ph value between 3.25 and 8.0 the cleaved enzyme is more active. substrate specificity is also influenced by proteolysis. only the cleaved form is able to hydrolyze unactivated substrates efficiently, and at ph >6 cleaved tracp acquires a marked preference for atp. the cleaved enzyme also has altered sensitivity to inhibitors. interestingl ...	2005	15950921
characterization and immunostimulatory activity of an (1-->6)-a-d-glucan from the root of ipomoea batatas.	the polysaccharide pspp (purified sweet potato polysaccharide), isolated and purified from the roots of ipomoea batatas, was found to be a glucan with a molecular weight of 53.2 kda and specific rotation of +115.0 degrees (ca. 0.80, h(2)o). on the basis of methylation analysis, periodate oxidation, smith degradation, infra-red spectroscopy, and (13)c nmr, the polysaccharide was confirmed as a (1-->6)-alpha-d-glucan. we evaluated the effects of polysaccharide pspp on the in vivo immune function o ...	2005	15953570
purification and characterization of a thermostable raw starch digesting amylase from a streptomyces sp. isolated in a milling factory.	a raw starch utilizing microbe was isolated from mud in a milling factory. the 16s ribosomal dna (rdna) sequencing and morphological properties of the strain indicated that it belongs to the genus streptomyces. a strongly raw starch digesting amylase was purified from the culture supernatant of the strain by chromatographic procedures. the specific activity of the enzyme was 11.7 u/mg, molecular mass 47 kda, optimum ph 6.0, and optimum temperature 50 to 60 degrees c. the enzyme showed sufficient ...	2005	15973037
the effect of phytic acid on the levels of blood glucose and some enzymes of carbohydrate and lipid metabolism.	in this study, six groups of rats were fed as follows: groups 1 and 2 were fed formulated diets supplemented with zinc or without zinc respectively. groups 3 and 4 were fed formulated diets supplemented with zinc plus phytic acid extracted from sweet potato (ipomea batatas) or commercial phytic acid respectively. groups 5 and 6 were fed formulated diets supplemented with phytic acid extract from sweet potato or commercial phytic acid respectively. the animals were fed for three weeks and then sa ...	2005	15999878
influence of synthetic and natural food dyes on activities of cyp2a6, ugt1a6, and ugt2b7.	synthetic or natural food dyes are typical xenobiotics, as are drugs and pollutants. after ingestion, part of these dyes may be absorbed and metabolized by phase i and ii drug-metabolizing enzymes and excreted by transporters of phase iii enzymes. however, there is little information regarding the metabolism of these dyes. it was investigated whether these dyes are substrates for cyp2a6 and udp-glucuronosyltransferase (ugt). the in vitro inhibition of drug-metabolizing enzymes by these dyes was ...	2005	16009655
soil and crop management experiments in the laboratory biosphere: an analogue system for the mars on earth(r) facility.	during the years 2002 and 2003, three closed system experiments were carried out in the "laboratory biosphere" facility located in santa fe, new mexico. the program involved experimentation of "hoyt" soy beans, (experiment #1) usu apogee wheat (experiment #2) and tu-82-155 sweet potato (experiment #3) using a 5.37 m2 soil planting bed which was 30 cm deep. the soil texture, 40% clay, 31% sand and 28% silt (a clay loam), was collected from an organic farm in new mexico to avoid chemical residues. ...	2005	16175677
enzymatic production of caffeic acid by koji from plant resources containing caffeoylquinic acid derivatives.	the effect of a koji (aspergillus awamori mut.) extract on the caffeoylquinic acid derivatives purified from sweetpotato (ipomoea batatas l.) leaves was examined to develop the mass production of caffeic acid. a koji extract hydrolyzed the caffeoylquinic acid derivatives, chlorogenic acid, 3,4-di-o-caffeoylquinic acid, 3,5-di-o-caffeoylquinic acid, 4,5-di-o-caffeoylquinic acid and 3,4,5-tri-o-caffeoylquinic acid, to caffeic acid. furthermore, the koji extract also converted the major polyphenoli ...	2005	16195601
cellulose production from glucose using a glucose dehydrogenase gene (gdh)-deficient mutant of gluconacetobacter xylinus and its use for bioconversion of sweet potato pulp.	a gene fragment encoding a putative pyrroloquinoline quinone glucose dehydrogenase (pqq gdh) was cloned from a bacterial cellulose (bc)-forming acetic acid bacterium, gluconacetobacter xylinus (=acetobacter xylinum) strain bpr 2001, which was isolated as a high bc producer when using fructose as the carbon source. a gdh-deficient mutant of strain bpr 2001, namely gd-i, was then generated via gene disruption using the cloned gene fragment. strain gd-i produced no gluconic acid but produced 4.1 g. ...	2005	16233811
growth inhibitory activities of crude extracts obtained from herbal plants in the ryukyu islands on several human colon carcinoma cell lines.	there is increasing interest in the use of herbs for the treatment of human diseases including cancer. therefore, the purpose of this study was to determine whether crude extracts obtained from 44 herbal plants in the ryukyu islands might contain components capable of inhibiting the growth of a variety of human colon carcinoma cell lines. leaves, roots and other parts of the plants were extracted with chloroform, and the crude extracts were dissolved in dimethylsulfoxide and used for the experim ...	2005	16235999
novel application of shochu distillery by-products to prophylaxis against mammary carcinogenesis induced by 7,12-dimethylbenz[a]anthracene in rats.	the effect of the heat-dried product of shochu distillery by-products (hsdb) derived from sweet potato on mammary carcinogenesis in rats was investigated. hsdb was fed at 2.5% or 5% of the total feed weight. dietary hsdb at the 5% level suppressed the incidence and number of tumors, and delayed the latency of mammary tumor development relative to the control diet. experiments were conducted to determine the relative polarity of the anticarcinogenic constituent(s). the number of tumors per tumor- ...	2005	16244440
cryptic polyadenylation of transcripts of an rna virus gene introduced into tobacco plants.	we constructed an expression vector for the coat protein (cp) gene and the 3' untranslated region (3' utr) of rna virus (sweet potato feathery mottle virus severe strain (spfmv-s)) lacking a foreign terminator. out of seven transgenic tobacco plants, expression of the transgene was observed in six plants. rt-pcr analysis revealed that the transcripts had a poly(a) tail, and in most of them, polyadenylation occurred on the 5' side of the 3' utr. these results suggest that the viral sequence conta ...	2005	16244460
production of mouse adiponectin, an anti-diabetic protein, in transgenic sweet potato plants.	adiponectin is a 30kda protein exclusively produced and secreted from adipocytes and as a cytokine has been found to link obesity, insulin resistance, and type 2 diabetes. production of biologically active adiponectin in large scale is desirable for pharmaceutical applications. mouse adiponectin cdna was used for developing transgenic sweet potato plants via agrobacterium-mediated transformation. the presence of the transgene was verified by pcr and dna gel blot analysis. further investigated we ...	2005	16255175
consumption of purple sweet potato leaves modulates human immune response: t-lymphocyte functions, lytic activity of natural killer cell and antibody production.	to study the immunological effects of physiological doses of purple sweet potato leaves (pspl).	2005	16270384
gateways to clinical trials.	gateways to clinical trials is a guide to the most recent clinical trials in current literature and congresses. the data in the following tables have been retrieved from the clinical trials knowledge area of prous science integrity, the drug discovery and development portal, http://integrity.prous.com. this issue focuses on the following selection of drugs: (-)-epigallocatechin gallate, (z)-4-hydroxytamoxifen; ad.muifn-beta ad-237, adalimumab, adefovir dipivoxil, agalsidase alfa, alemtuzumab, al ...	2005	16273137
the broadly insecticidal photorhabdus luminescens toxin complex a (tca): activity against the colorado potato beetle, leptinotarsa decemlineata, and sweet potato whitefly, bemisia tabaci.	toxin complex a (tca), a high molecular weight insecticidal protein complex produced by the entomopathogenic bacterium photorhabdus luminescens, has been found to be orally toxic to both the colorado potato beetle, leptinotarsa decemlineata, and the sweet potato whitefly, bemisia tabaci biotype b. the 48 hour lc50 for tca against neonate l. decemlineata was found to be 2.7 ppm, and the growth of 2nd instar l. decemlineata exposed to tca for 72 hours was almost entirely inhibited at concentration ...	2005	17119614
intersterility, morphology and taxonomy of ceratocystis fimbriata on sweet potato, cacao and sycamore.	ceratocystis fimbriata is a large, diverse complex of species that cause wilt-type diseases of many economically important plants. previous studies have shown that isolates in three monophyletic lineages within the latin american clade of c. fimbriata are host-specialized to cacao (theobroma cacao), sweet potato (ipomoea batatas) and sycamore (platanus spp.), respectively. we paired testers of opposite mating type from isolates of these lineages to find intersterility groups. two intersterility ...	2005	16389957
palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring.	three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (rptp), anionic sweet potato peroxidase (spp), or cationic horseradish peroxidase (hrp-c) on spectroscopic graphite electrodes. the resulting h(2)o(2)-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as ph dependency, i(max), k(m)(app), detection limit, linear range, operational and storage s ...	2005	16242613
activation tagging of a gene for a protein with novel class of cct-domain activates expression of a subset of sugar-inducible genes in arabidopsis thaliana.	in the current studies, we examined sugar-inducible gene expression using the arabidopsis thaliana line sgsl, which carries luciferase (luc) and beta-glucuronidase (gus) reporter genes under the control of a 210-bp promoter derived from the sweet potato sporamin gene (spo(min)). we isolated an enhancer activation-tagged mutant of this line that showed high-level expression of luc and gus under non-inducing low-sugar conditions. the activator ofspo(min)::luc2 (asml2) gene located close to the enh ...	2005	15960623
activator of spomin::luc1/wrinkled1 of arabidopsis thaliana transactivates sugar-inducible promoters.	we isolated an enhancer activation-tagged mutant of arabidopsis thaliana line sgsl carrying the luciferase (luc) gene under control of a short sugar-inducible promoter derived from a sweet potato sporamin gene (spomin) that showed high level expression of luc under non-inducing conditions. the activator of spomin::luc1 (asml1) gene located downstream of the enhancer encoded an apetala2 (ap2)-type ap2 domain protein, and this gene was shown recently to be responsible for the wrinkled1 mutation wh ...	2005	15753106
analysis of genes developmentally regulated during storage root formation of sweet potato.	to identify the genes involved in storage root formation of sweet potato (ipomoea batatas), we performed a simplified differential display analysis on adventitious roots at different developmental stages of the storage root. the expression patterns were confirmed by semiquantitative rt-pcr analyses. as a result, 10 genes were identified as being developmentally regulated and were named srf1-srf10. the expression of srf1, srf2, srf3, srf5, srf6, srf7, and srf9 increased during storage root format ...	2005	15700424
analysis of a sugar response mutant of arabidopsis identified a novel b3 domain protein that functions as an active transcriptional repressor.	a recessive mutation hsi2 of arabidopsis (arabidopsis thaliana) expressing luciferase (luc) under control of a short promoter derived from a sweet potato (ipomoea batatas) sporamin gene (spo(min)luc) caused enhanced luc expression under both low- and high-sugar conditions, which was not due to increased level of abscisic acid. the hsi2 mutant contained a nonsense mutation in a gene encoding a protein with b3 dna-binding domain. hsi2 and two other arabidopsis proteins appear to constitute a novel ...	2005	15894743
unravelling the genetic diversity of the three main viruses involved in sweet potato virus disease (spvd), and its practical implications.	summary sweetpotato (ipomoea batatas) is a widely grown food crop, in which the most important diseases are caused by viruses. genetic variability of three widely distributed sweetpotato viruses was analysed using data from 46 isolates of sweet potato feathery mottle virus (spfmv), 16 isolates of sweet potato mild mottle virus (spmmv) and 25 isolates of sweet potato chlorotic stunt virus (spcsv), of which 19, seven and six isolates, respectively, are newly characterized. division of spfmv into f ...	2005	20565651
overview and analysis of the polyprotein cleavage sites in the family potyviridae.	summary the genomes of plant viruses in the family potyviridae encode large polyproteins that are cut by virus-encoded proteases into ten mature proteins. three different types of protease have been identified, each of which cuts at sites with a distinctive sequence pattern. the experimental evidence for this specificity is reviewed and the cleavage site patterns are compiled for all sequenced species within the family. seven of the nine cleavage sites in each species are cut by the viral nia-pr ...	2005	20565672
heterologous expression of the mi-1.2 gene from tomato confers resistance against nematodes but not aphids in eggplant.	the mi-1.2 gene in tomato (solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (nblrr) class of plant resistance genes, and confers resistance against root-knot nematodes (meloidogyne spp.), the potato aphid (macrosiphum euphorbiae), and the sweet potato whitefly (bemisia tabaci). mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. in this study, eggplant ...	2006	16610741
determination of pharmacologically active ingredients in sweet potato (ipomoea batatas l.) by capillary electrophoresis with electrochemical detection.	sweet potato (ipomoea batatas l.), in which vitamin c, chlorogenic acid, caffeic acid, quercetin, and rutin are abundant, is one of the functional food products aimed at introducing human dietary ingredients that aid specific body functions in addition to being nutritious. a method based on capillary electrophoresis with electrochemical detection (ce-ed) to qualitatively and quantitatively determine the pharmacologically active ingredients in sweet potato has been developed by our group. the eff ...	2006	16390172
a tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio.	the sequencing of the genome of ralstonia solanacearum[salanoubat m, genin s, artiguenave f, et al. (2002) nature 415, 497-502] revealed several genes that putatively code for polyphenol oxidases (ppos). this soil-borne pathogenic bacterium withers a wide range of plants. we detected the expression of two ppo genes (accession numbers np_518458 and np_519622) with high similarity to tyrosinases, both containing the six conserved histidines required to bind the pair of type-3 copper ions at the ac ...	2006	16403014
monascus fermentation of dioscorea for increasing the production of cholesterol-lowering agent--monacolin k and antiinflammation agent--monascin.	monacolin k, an inhibitor for cholesterol synthesis, is the secondary metabolite of monascus species. the formation of the secondary metabolites of the monascus species is affected by cultivation environment and method. this research uses sweet potato (ipomoea batatas), potato (solanum tuberosum), casava (manihot esculenta), and dioscorea (dioscorea batatas) as the substrates and discusses the best substrate to produce monacolin k. the results show that monascus purpureus ntu 301, with dioscorea ...	2006	16568313
irradiation as a methyl bromide alternative for postharvest control of omphisa anastomosalis (lepidoptera: pyralidae) and euscepes postfasciatus and cylas formicarius elegantulus (coleoptera: curculionidae) in sweet potatoes.	irradiation studies were conducted with three sweet potato, ipomoea batatas (l.) lam., pests to determine an effective dose for quarantine control. dose-response tests indicated that the most radiotolerant stage occurring in roots was the pupa of sweetpotato vine borer, omphisa anastomosalis (guenee), and the adult of west indian sweetpotato weevil, euscepes postfasciatus (fairmaire), and sweetpotato weevil, cylas formicarius elegantulus (summers). in large-scale confirmatory tests, irradiation ...	2006	16573320
complete nucleotide sequence of the rna2 of the crinivirus tomato chlorosis virus.	the complete sequence of genomic rna2 of tomato chlorosis virus (tocv; genus crinivirus, family closteroviridae), isolate at80/99 from spain, was determined and compared with those from the other members of the genus sequenced to date. rna2 is 8244 nucleotides (nt) long and putatively encodes nine orfs that encompass the hallmark gene array of the family closteroviridae, which includes a heat shock protein 70 family homologue, a 59 kda protein, the coat protein, and a diverged coat protein. phyl ...	2006	16374719
a new mads-box gene (ibmads10) from sweet potato (ipomoea batatas (l.) lam) is involved in the accumulation of anthocyanin.	a new mads-box gene designated as ibmads10 was cloned and its expression was characterized from sweet potato (ipomoea batatas (l.) lam.) cv. beniazuma. the deduced amino acid sequence of the gene indicated high homology with members of the mads-box family of transcription factors. ibmads10 shares high amino acid sequence similarity with the defh28 of antirrhinum majus (64%) and with bpmads4 of betula pendula (61%) of the squa subfamily. southern blot analysis revealed that the ibmads10 is presen ...	2006	16333667
identification and localization of a rickettsia sp. in bemisia tabaci (homoptera: aleyrodidae).	whiteflies (homoptera: aleyrodidae) are sap-sucking insects that harbor "candidatus portiera aleyrodidarum," an obligatory symbiotic bacterium which is housed in a special organ called the bacteriome. these insects are also home for a diverse facultative microbial community which may include hamiltonella, arsenophonus, fritchea, wolbachia, and cardinium spp. in this study, the bacteria associated with a b biotype of the sweet potato whitefly bemisia tabaci were characterized using molecular fing ...	2006	16672513
effectiveness of two insect growth regulators against bemisia tabaci (gennadius) (homoptera: aleyrodidae) and helicoverpa armigera (hübner) (lepidoptera: noctuidae) and their impact on population densities of arthropod predators in cotton in pakistan.	field efficacies of two insect growth regulators (igrs) at two recommended application rates, buprofezin at 370 and 555 g ai ha(-1) and lufenuron at 37 and 49 g ai ha(-1), were determined against the sweet potato whitefly, bemisia tabaci (gennadius), and the cotton bollworm, helicoverpa armigera (hübner), in experimental plots of cotton at the directorate of cotton research, faisalabad, pakistan. adverse effects of the igrs on populations of associated arthropod predators, namely geocorids, chry ...	2006	16862616
food processing methods influence the glycaemic indices of some commonly eaten west indian carbohydrate-rich foods.	glycaemic index (gi) values for fourteen commonly eaten carbohydrate-rich foods processed by various methods were determined using ten healthy subjects. the foods studied were round leaf yellow yam (dioscorea cayenensis), negro and lucea yams (dioscorea rotundata), white and sweet yams (dioscorea alata), sweet potato (solanum tuberosum), irish potato (ipomoea batatas), coco yam (xanthosoma spp.), dasheen (colocasia esculenta), pumpkin (cucurbita moschata), breadfruit (artocarpus altilis), green ...	2006	16925852
electrophysiological and behavioral responses of a cuban population of the sweet potato weevil to its sex pheromone.	the sex pheromone of feral sweet potato weevils cylas formicarius elegantulus from cuba was found, via solid-phase microextraction analysis, to be identical to (z)-3-dodecenyl (e)-2-butenoate, a previously reported compound. females emitted 20 pg pheromone d(-1). in scanning electron microscopy studies carried out on the male antenna, we identified several types of sensilla: sensilla trichoidea of type 1 (st1) as long hairs (100-150 microm), sensilla trichoidea of type 2 as short hairs (50-60 mi ...	2006	16977491
determination of acylated anthocyanin in human urine after ingesting a purple-fleshed sweet potato beverage with various contents of anthocyanin by lc-esi-ms/ms.	eighty-seven healthy volunteers ingested a purple-fleshed sweet potato beverage with various contents of anthocyanin (beverage a; 22.1 mg/250 ml, b; 107.8, c; 84.9). an acylated anthocyanin, peonidin 3-caffeoylsophoroside-5-glucoside, was detected in the urine 2 h after ingestion. the concentrations were 15.1+/-2.2 microg/l of urine (mean+/-sem), 46.6+/-5.3, and 53.3+/-2.2 for beverages a, b, and c respectively.	2006	17031052
sweet potato [ipomoea batatas (l.) lam].	among the available transformation methods reported on sweet potato, agrobacterium tumefaciens-mediated transformation is more successful and desirable. stem explants have shown to be ideal for the transformation of sweet potato because of their ready availability as explants, the simple transformation process, and high-frequency-regeneration via somatic embryogenesis. under the two-step kanamycin-hygromycin selection method and using the appropriate explants type (stem explants), the efficiency ...	2006	17033049
improved dry-fleshed sweetpotato genotypes resistant to insect pests.	thirty-five mostly dry-fleshed sweetpotato, ipomoea batatas (l.) lam. (convolvulaceae), genotypes from the usda-ars/clemson university sweetpotato breeding program were evaluated in nine field experiments at the u.s. vegetable laboratory, charleston, sc, from 1998 to 2004. there were highly significant entry effects for percentage of uninjured roots; wireworm, diabrotica, and systena (wds) index; percentage of roots damaged by sweetpotato weevil, cylas formicarius elegantulus (summers); percenta ...	2006	17066825
regulation of osteoclastogenesis by simon extracts composed of caffeic acid and related compounds: successful suppression of bone destruction accompanied with adjuvant-induced arthritis in rats.	simon extracts are vitamin k(1)-rich food materials extracted from the leaves of the simon sweet potato. although vitamin k is known to stimulate bone formation, we postulated that simon extracts also contain unknown biological compounds having the ability to regulate bone resorption. here we prepared the vitamin k-free fraction from the simon extracts and investigated the ability of this fraction on the differentiation of osteoclasts. a remarkable inhibitory effect of osteoclastogenesis was obs ...	2006	16205940
[sweet potato in pre-columbian polynesia--an overview].	an overview is presented on the reports available so far on sweet potato, ipomoea batatas, cultivated widely in polynesia in the pre-columbian era, with reference to possible ways and presumptive dates of transfer from the americas to polynesia, such as (1) polynesian navigators' travel to peru, (2) peruvian fishermen's drift westward, (3) vessel drift, (4) seed drift, (5) root-tuber drift, and (6) transport by birds. the author supports the case (1) as most plausible. ganshu or ganchu described ...	2006	17139158
site-specific phosphorylation of l-form starch phosphorylase by the protein kinase activity from sweet potato roots.	a 78-amino acid insertion (l78) is found in the low-affinity type (l-form) of starch phosphorylase (l-sp, ec 2.4.1.1). this insertion blocks the starch-binding site on the l-sp molecule, and it decreases the binding affinity of l-sp toward starch. the computational analysis of the amino acid sequence on l78 predicts several phosphorylation sites at its ser residues. indeed, from the immunoblotting results using antibodies against phosphoamino acids, we observed that the purified l-sp from mature ...	2006	16151850
recombinant purple acid phosphatase isoform 3 from sweet potato is an enzyme with a diiron metal center.	purple acid phosphatases (paps) from sweet potato (sp) have been classified on the basis of their primary structure and the dinuclear metal center into isoforms sppap1 [fe(iii)-zn(ii)] and sppap2 [fe(iii)-mn(ii)]; for sppap3 only the cdna is known. with the aim of unraveling the character of the dinuclear metal center we report here the characterization of this isoform at the protein level. we cloned sppap3 cdna in a baculovirus and overexpressed this enzyme in sf9 insect cells. preparation of r ...	2006	16623702
within-plant distribution and sampling of single and mixed infestations of bemisia tabaci and trialeurodes vaporariorum (homoptera: aleyrodidae) in winter tomato crops.	in several areas of spain, the greenhouse whitefly, trialeurodes vaporariorum (westwood), and the sweet potato whitefly, bemisia tabaci (gennadius), coexist in tomato, lycopersicon esculentum miller. for integrated pest management decision-making, it is important to know the abundance of each species, because they exhibit different abilities to transmit viruses, are susceptible to different biological control agents, and have different responses to insecticides. this study was conducted to provi ...	2006	16686130
new gene construction strategy in t-dna vector to enhance expression level of sweet potato sporamin and insect resistance in transgenic brassica oleracea.	sporamin, an abundant storage protein in tuberous roots of sweet potato, possesses strong inhibitory activity against trypsin and pest-resistance. to promote consistent high-level expression of sporamin and insect resistance in transgenic brassica plants, a wound-responsive sporamin promoter (pspoa) alone or combined with matrix-attached-region-like dna segment (spomar) were constructed for driving sporamin cdna. the results showed the transgenic plants containing pspoa-drived sporamin and spoma ...	2006	22980206
uptake and transformation of phenol and chlorophenols by hairy root cultures of daucus carota, ipomoea batatas and solanum aviculare.	hairy root cultures of daucus carota l., ipomoea batatas l. and solanum aviculare forst were investigated for their susceptibility to the highly toxic pollutants phenol and chlorophenols and for the involvement of inherent peroxidases in the removal of phenols from liquid media. roots of d. carota grew normally in medium containing 1000 micromol l(-1) of phenol, whilst normal growth of roots of i. batatas and s. aviculare was only possible at levels up to 500 micromol l(-1). in the presence of c ...	2006	16364403
two sweetpotato adp-glucose pyrophosphorylase isoforms are regulated antagonistically in response to sucrose content in storage roots.	the transcriptional regulation of adp-glucose pyrophosphorylase (agpase) genes in detached leaves in response to exogenous sucrose has been investigated earlier; however the effects of endogenous sucrose on agpase gene transcription in leaves or starch-accumulating tissues have not yet been determined. we therefore have investigated the relationship between endogenous sucrose content in the storage tissues of sweetpotato (ipomoea batatas cv. yulmi) and the rate of transcription of the two sweetp ...	2006	16338103
prehistoric agricultural depletion of soil nutrients in hawai'i.	we investigated the fate of soil nutrients after centuries of indigenous dryland agriculture in hawai'i using a coupled geochemical and archaeological approach. beginning approximately 500 years ago, farmers began growing dryland taro and sweet potato on the leeward slopes of east maui. their digging sticks pierced a subsurface layer of cinders, enhancing crop access to the soil water stored below the intact cinders. cultivation also catalyzed nutrient losses, directly by facilitating leaching o ...	2006	16832047
occurrence and cytological mechanism of 2n pollen formation in a tetraploid accession of ipomoea batatas (sweet potato).	we evaluated a 4x accession of ipomoea batatas (l.) lam. and four 2x accessions of ipomoea triloba for 2n pollen production. approximately 90% of the genotypes of accession 81.2 (i. batatas, 4x) produced 2n pollen with different frequencies. in contrast, none of the genotypes of i. triloba produced 2n pollen. the diameter of the 2n pollen was approximately 30% ((3) sqrt 2) larger than that of the n pollen, making it easy to identify, measure, and quantify. the correlation (r = 0.93**) between th ...	2006	12195034
analysis of sphingolipids in potatoes (solanum tuberosum l.) and sweet potatoes (ipomoea batatas (l.) lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (hplc-esi-ms/ms).	ceramides and glucocerebrosides of potatoes (solanum tuberosum l.) and sweet potatoes (ipomoea batatas (l.) lam.) were analyzed using rp-hplc-esi-ms/ms. ceramides and glucocerebrosides containing the three different long-chain bases 4,8-sphingadienine (d18:2(delta4,delta8)), 4-hydroxy-8-sphingenine (t18:1(delta8)), and 8-sphingenine (d18:1(delta8)) acylated to saturated and unsaturated hydroxy- and nonhydroxy fatty acids with 16-26 carbon atoms were detected. for ceramides and glucocerebrosides ...	2006	17103377
new polyphenol derivative in ipomoea batatas tubers and its antioxidant activity.	four different polyphenolic compounds were isolated by chromatographic methods from methanolic and hydromethanolic extracts of ipomoea batatas tuber flour. on the basis of uv, mass, and nmr analysis procedures, the structure of the isolated compounds were determined as 4,5-di-o-caffeoyldaucic acid (1), 4-o-caffeoylquinic acid (2), 3,5-di-o-caffeoylquinic acid (3), and 1,3-di-o-caffeoylquinic acid (4). to the best of our knowledge, this is the first report of isolation and characterization of com ...	2006	17090114
[drought tolerance of transgenic sweet potato expressing both cu/zn superoxide dismutase and ascorbate peroxidase].	two strains, cu/zn sod and apx gene transferred sweet potato (ts) and non-transgenic sweet potato (ipomoea batatas l.) (nt), were used as experimental materials to study the drought tolerance under three different degrees of water stress: 0, -0.44 mpa, -0.78 mpa. the results showed that activities of cu/zn sod and apx increased under -0.44 mpa and decreased under -0.78 mpa (fig. 2), p(n), g(s) and leaf water content decreased, c(i) increased, then decreased under water stress (fig. 6), but under ...	2006	16957397
the origin and evolution of sweet potato (ipomoea batatas lam.) and its wild relatives through the cytogenetic approaches.	the distribution and organization of 5s and 18s-5.8s-26s (18s) rdna were studied in 10 varieties of hexaploid ipomoea batatas, five accessions of tetraploid ipomeoa trifida, and six related species (five diploids, i. trifida, i. triloba, i. tiliacea, i. leucantha and i. setosa and one tetraploid, i. tabascana), by using fluorescence in situ hybridization (fish). the fish data obtained indicated that polyploidization was followed by decrease in the number of 18s rdna loci in higher ploidy level a ...	2006	22980213
seven new aminoacyl sugars in ipomoea batatas.	an analysis of the polar extracts from sweet potato ipomoea batatas (convolvulaceae) led to the isolation of seven unknown aminoacyl sugars. on the basis of 1d, 2d nmr, and mass spectrometry data, the structures of the compounds were elucidated as: beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-valyl]-glucopyranoside (1), beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-tyrosyl]-glucopyranoside (2), beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-threonyl]-glucopyranoside (3), beta-d-fructofuranosyl-( ...	2006	16881721
starch-branching enzyme i gene (ibsbei) from sweet potato (ipomoea batatas); molecular cloning and expression analysis.	the cdna of the starch-branching enzyme i gene (ibsbei) in the sweet potato (ipomoea batatas) has been cloned and sequenced. the ibsbei amino acid sequence was 81% identical to that of potato stsbei. dna gel-blot analyses demonstrated that at least two copies of ibsbei are present in the sweet potato genome. ibsbei was strongly expressed in tuberous roots. transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased ...	2006	16802100
molecular cloning and characterization of a granulin-containing cysteine protease spcp3 from sweet potato (ipomoea batatas) senescent leaves.	granulins are a family of evolutionarily ancient proteins that are involved in regulating cell growth and division in animals. in this report a full-length cdna, spcp3, was isolated from senescent leaves of sweet potato (ipomoea batatas). spcp3 contains 1389 nucleotides (462 amino acids) in its open reading frame, and exhibits high amino acid sequence homologies (ca. 64-73.6%) with several plant granulin-containing cysteine proteases, including potato, tomato, soybean, kidney bean, pea, maize, r ...	2006	16777534
changes in caffeic acid derivatives in sweet potato (ipomoea batatas l.) during cooking and processing.	there was an obvious decrease in caffeic acid derivatives during the boiling of cube-shaped blocks of sweet potatoes. they also decreased in a mixture of freeze-dried sweet-potato powder and water maintained at room temperature. ascorbic acid prevented the decrease, supporting the occurrence of an enzyme reaction with polyphenol oxidase (ppo). 5-o-caffeoylquinic acid (5-cqa, "3-o-caffeoylquinic acid" as a trivial name) and 3,5-di-o-caffeoylquinic acid (3,5-cqa), major phenolic compounds of sweet ...	2006	16428835
medium alterations improve regrowth of sweet potato (ipomoea batatas [l.] lam.) shoot tips cryopreserved by vitrification and encapsulation-dehydration.	in vitro grown sweet potato (ipomoea batatas [l.] lam.) shoot tips were successfully cryopreserved by both solution based and encapsulation-dehydration vitrification methods. improved recovery medium enhanced recovery for both vitrification procedures. the effects of sucrose preculture, cryoprotectant preculture and post-warm recovery media on regrowth following ln exposure were investigated. sucrose preculture was critical for the survival of sweet potato shoot tips cooled to ca. -200 degrees c ...	2006	11788880
differential expression of 10 sweetpotato peroxidases in response to sulfur dioxide, ozone, and ultraviolet radiation.	secretory class iii plant peroxidase (pod, ec 1.11.1.7) is believed to function in diverse physiological processes, including responses to various environmental stresses. to understand the function of each pod in terms of air pollutants and uv radiation, changes in pod activity and expression of 10 pod genes isolated from cell cultures of sweetpotato (ipomoea batatas) were investigated in the leaves of sweetpotato after treatment with sulfur dioxide (so(2) 500ppb, 8h/day for 5 days), ozone (o(3) ...	2007	17870589
changes in polyphenolic content and radical-scavenging activity of sweet potato (ipomoea batatas l.) during storage at optimal and low temperatures.	polyphenolic content and radical-scavenging activities (rsa) of four sweet potato (ipomoea batatas l.) cultivars were characterized after storage at optimal (15 degrees c) or low temperature (5 degrees c) for 0, 13, 26, and 37 days. the polyphenolic content increased during storage in three cultivars but not in 'murasakimasari'. the change in 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity (dpph-rsa) correlated very well with polyphenolic content. the increases in polyphenolics and the ...	2007	18038989
methodology optimization for quantification of total phenolics and individual phenolic acids in sweetpotato (ipomoea batatas l.) roots.	phenolic acids are one of the several classes of naturally occurring antioxidant compounds found in sweetpotatoes. simplified, robust, and rapid methodologies were optimized to quantify total and individual phenolic acids in sweetpotato roots. total phenolic acid content was quantified spectrophotometrically using both folin-denis and folin-ciocalteu reagents. the folin-ciocalteu reagent gave an overestimation of total phenolic acids due to the absorbance of interfering compounds (that is, reduc ...	2007	17995640
novel galactolipids from the leaves of ipomoea batatas l.: characterization by liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight tandem mass spectrometry.	sixteen novel and ten known galactolipids have been isolated and characterized from the leaves of ipomoea batatas l. (sweet potato) using an analytical method based on high-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight tandem mass spectrometry. using this technique, the structures and regiochemistries of the fatty acyl groups and the positions of the double bonds on the acyl chains were determined. sugar moieties were identified by analysis of o ...	2007	17988089
batatins i and ii, ester-type dimers of acylated pentasaccharides from the resin glycosides of sweet potato.	batatins i (1) and ii (2), two ester-type dimers of acylated pentasaccharides, have been isolated by recycling hplc from the hexane-soluble extract of sweet potato (ipomoea batatas var. batatas). the structures were elucidated by a combination of high-resolution nmr spectroscopy and mass spectrometry. complete analysis and unambiguous assignments of the 1h and 13c nmr spectra of 1 and 2 were achieved by 2d shift correlation measurements. the glycosidic acid forming each branched pentasaccharide ...	2007	17488129
carotenoids with a 5,6-dihydro-5,6-dihydroxy-beta-end group, from yellow sweet potato "benimasari", ipomoea batatas lam.	a series of carotenoids with a 5,6-dihydro-5,6-dihydroxy-beta-end group, named ipomoeaxanthins a (1), b (2), c1 (3) and c2 (4) were isolated from the flesh of yellow sweet potato "benimasari", ipomoea batatas lam. their structures were determined to be (5r,6s,3'r)-5,6-dihydro-beta,beta-carotene-5,6,3'-triol (1), (5r,6s,5'r,6's)-5,6,5',6'-tetrahydro-beta,beta-carotene-5,6,5'6'-tetrol (2), (5r,6s,5'r,8'r)-5',8'-epoxy-5,6,5',8'-tetrahydro-beta,beta-carotene-5,6-diol (3), and (5r,6s,5'r,8's)-5',8'-e ...	2007	17466348
enzymatic reverse fia method for total phenols determination in urine samples.	a reverse flow injection spectrophotometric enzymatic method is proposed to quantify total phenols in urine samples. the polyphenol oxidase (ppo; ec 1.14.18.1) obtained as a crude extract from sweet potato root (ipomoea batatas) was used as enzymatic catalyze. the detection limit, the sample throughput and relative standard deviation were 7.7mgl(-1) of total phenols, 49h(-1) and 0.9%, respectively. the method was applied to real samples and a recovery study was carried out in order to its valida ...	2007	19071485
triterpene saponin hemi-biosynthesis of a leaf beetle's (platyphora kollari) defensive secretion.	the adults of the leaf beetle platyphora kollari (chrysomelidae) are able to metabolise the oleanane triterpene beta-amyrin (1) into the glycoside 3-o-beta-d: -glucopyranosyl-(1-->4)-beta-d: -glucuronopyranosyl-hederagenin (2) that is stored in their defensive glands. the aim of this study was to test the hypothesis that oleanolic acid (3) is an intermediate in the conversion of 1 into 2 and to check whether the sequestration of pentacyclic triterpenes is selective in favour of beta-amyrin (1). ...	2007	17354008
growth inhibition and induction of apoptosis in nb4 promyelocytic leukemia cells by trypsin inhibitor from sweet potato storage roots.	the objective of this study was to investigate the antiproliferative effect and the mechanism of trypsin inhibitor (ti) from sweet potato [ipomoea batatas (l.) lam. 'tainong 57'] storage roots on nb4 promyelocytic leukemia cells. the results showed that ti inhibited cellular growth of nb4 promyelocytic leukemia cells in a time-dependent and dose-dependent manner, and treatment for 72 h induced a marked inhibition of cellular growth, showing an ic50 of 57.1 +/- 8.26 microg/ml. ti caused cell cycl ...	2007	17328557
resistance of sweetpotato genotypes to adult diabrotica beetles.	production of sweetpotatoes, ipomoea batatas (l.) lam. (convolvulaceae), is limited by several insect pests, including diabrotica spp. (coleoptera: chrysomelidae), and new integrated pest management (ipm) techniques for this crop are needed. host plant resistance is one attractive approach that fits well into ipm programs. a host plant resistance research program typically depends on reliable bioassay procedures to streamline evaluation of germplasm. thus, a bioassay technique was developed for ...	2007	17461084
global relationships of bemisia tabaci (hemiptera: aleyrodidae) revealed using bayesian analysis of mitochondrial coi dna sequences.	bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a species complex that is one of the most devastating agricultural pests worldwide and affects a broad range of food, fiber and ornamental crops. unfortunately, using parsimony and neighbor joining methods, global phylogenetic relationships of the major races/biotypes of b. tabaci remain unresolved. aside from the limitations of these methods, phylogenetic analyses have been limited to only small subsets of the global collection of b. tabaci ...	2007	17627853
toxicity and feeding deterrence of crude toxin extracts of lecanicillium (verticillium) lecanii (hyphomycetes) against sweet potato whitefly, bemisia tabaci (homoptera: aleyrodidae).	crude toxins [toxin(v3450) and toxin(vp28,) extracted from lecanicillium (verticillium) lecanii (zimmermann) gams & zare strain v3450 and vp28 respectively] were tested for contact toxicity, feeding deterrence and repellent activity against the sweet potato whitefly, bemisia tabaci (gennadius) (homoptera: aleyrodidae). both toxins showed ovicidal activity to eggs, with lc(50) values of 447 and 629 mg l(-1) respectively. nymphs of b. tabaci were the most susceptible stages (lc(50) values were cal ...	2007	17315271
ecdysteroids and juvenile hormones of whiteflies, important insect vectors for plant viruses.	ecdysteroids and juvenile hormones (jhs) regulate many physiological events throughout the insect life cycle, including molting, metamorphosis, ecdysis, diapause, reproduction, and behavior. fluctuation of whitefly ecdysteroid levels and the identity of the whitefly molting hormone (20-hydroxyecdysone) have only been reported within the last few years. an ecdysteroid commitment peak that is associated with the reprogramming of tissues for a metamorphic molt in many holometabolous and some hemime ...	2007	17258230
tropical whitefly ipm project.	the tropical whitefly ipm project (twfp) is an initiative of the systemwide ipm programme of the consultative group on international agricultural research (cgiar), financed by the department for international development (dfid) of the united kingdom, the danish international development agency (danida), the united states department of agriculture (usda) and agency for international development (usaid), the australian centre for international agricultural research (aciar), and the new zealand age ...	2007	17222696
critical feeding periods for last instar nymphal and pharate adults of the whiteflies, trialeurodes vaporariorum and bemisia tabaci.	a critical feeding period is the time after which 50% of a given species of insect can be removed from its food source and complete development by undergoing adult eclosion. the critical feeding period was determined for the greenhouse white fly, trialeurodes vaporariorum, and the sweet potato whitefly, bemisia tabaci (biotype b) (homptera/hemiptera: aleyrodidae). fourth (last) instar and pharate adult whiteflies were removed from green bean leaves, staged, placed on filter paper in small petri ...	2007	20302459
[a new species of thrip, genus frankliniella (group cephalica; thysanoptera: thripidae) from costa rica].	a new species of the frankliniella cephalica group, from central costa rica, is described under the name "frankliniella morerai n.sp.". it is closely related to frankliniella sandovalensis. both species have translucent bodies, but the new species can be distinguished by these characteristics: setae io ii in position 1/2, a complete comb on abdominal segment viii, antennal segments without coloration and setae po iv longer. it was found in low density in crops of chilli, tomato, lettuce, sweet p ...	2007	17354432
in vitro availability of some essential minerals in commonly eaten processed and unprocessed caribbean tuber crops.	the levels of three essential minerals ca, fe and mg and the extent of their availability were assessed in four commonly eaten caribbean tuber crops [dasheen (xanthosoma spp.), irish potato (solanum tuberosum), sweet potato (ipomoea batatas) and yellow yam (dioscorea cayenensis)] in their processed and unprocessed states. calcium was highest in cooked dasheen (5150+/-50 mg/kg) while magnesium was highest in uncooked irish potato (3600+/-200 mg/kg). there was no significant loss of calcium from t ...	2007	16758116
analysis of transitive rna silencing after grafting in transgenic plants with the coat protein gene of sweet potato feathery mottle virus.	we have previously reported the graft transmission of target specificity for rna silencing using transgenic nicotiana benthamiana plants expressing the coat protein gene (cp, including the 3' non-translated region) of sweet potato feathery mottle virus. transgenic plants carrying the 5' 200 and 400 bp regions of cp were newly produced. from these plants, two silenced and two non-silenced lines were selected to investigate the manifestation of transitive rna silencing by graft experiments. non-si ...	2007	17160454
sequence analysis of the entire rna genome of a sweet potato chlorotic fleck virus isolate reveals that it belongs to a distinct carlavirus species.	since the paucity of information on sweet potato chlorotic fleck virus (spcfv) had precluded its classification, we have determined the complete nucleotide sequence of the single-stranded rna genome of a ugandan isolate of spcfv. the genome is 9104 nucleotides long (excluding the poly(a) tail) and potentially includes six open reading frames (orfs). based on genomic organisation and sequence similarity, spcfv appears to be a member of the genus carlavirus (family flexiviridae). however, spcfv is ...	2007	17187295
growth suppression of human cancer cells by polyphenolics from sweetpotato (ipomoea batatas l.) leaves.	sweetpotato leaves (ipomoea batatas l.) contain a high content of polyphenolics that consist of caffeic acid, chlorogenic acid, 3,4-di-o-caffeoylquinic acid, 3,5-di-o-caffeoylquinic acid, 4,5-di-o-caffeoylquinic acid, and 3,4,5-tri-o-caffeoylquinic acid. we investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. the human cancer cells used in this research included a stomach cancer (kato iii), a colon cancer (dld-1), ...	2007	17199331