Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of a membrane antigen from rabbit testis and sperm isolated by using monoclonal antibodies and effect of its antiserum on fertility. | an antigen was isolated from deoxycholate-solubilized rabbit testis and sperm by using an immunosorbent column containing igg from a monoclonal antibody (8c10.5) that inhibits fertility. elution was by stepwise increases in ph (8.0, 10.0, and 11.4), with the ph 11.4 fraction after recycling through the column showing a single band at 63 kilodaltons in slab nadodso4/page with a silver stain. the antigen molecule was composed of two subunits, which on two-dimensional page showed many spots within ... | 1984 | 6366792 |
| light and electron microscope analysis of lectin binding to adult rat liver in situ. | a comprehensive mapping of lectin receptors on adult rat liver in situ was performed at light and ultrastructural levels by using 12 biotin-labeled lectins and an avidin-biotin-peroxidase complex. in addition, concanavalin a conjugated directly to peroxidase was utilized to study intracellular membrane glycoconjugates. to achieve optimal preservation of these membrane sugar moieties, several fixatives and fixation procedures were evaluated. a periodate-lysin-paraformaldehyde combination provided ... | 1984 | 6368972 |
| presence of hla-d/dr antigens on the membrane of breast tumour cells. | hla-d/dr (ia) glycoproteins were identified in human breast carcinoma and normal mammary gland cells by means of an anti-ia monoclonal antibody. two techniques were used: (1) immunoperoxidase staining performed on histological sections and (2) ia glycoproteins were isolated as follows: firstly by radioactive labelling of isolated cells, then by filtration on sephadex g25, followed by lens culinaris chromatography, and immune complex formation and then elution on protein a-sepharose. lastly, the ... | 1984 | 6370519 |
| the effect of lentil lectin treatment on the survival of rat skin allografts in strain combinations with different genetic disparity. | the effect of lentil lectin on the survival of skin allografts was investigated in six rat strain combinations with different genetic disparities between the donors and recipients. lca was administered i.p. (50 mg/kg) to the recipients daily, starting with the day of transplantation (day 0) until graft rejection, except that the doses on days 1, 3, 5, 7, and 9 were given i.v. (25 mg/kg). lca treatment was more efficient in prolongation of skin graft survival time in congenic strain combinations ... | 1984 | 6373390 |
| immunopharmacologic profiles of a thiol protease inhibitor, l-trans-dicyclohexyl epoxysuccinate. | ep-1, l-trans-dicyclohexyl epoxysuccinate, is a synthetic and specific inhibitor of thiol proteases. the effects of this inhibitor on some immunological parameters were examined in normal and immunity-impaired mice and rats. in the cultures of splenocytes obtained from the mice treated with ep-1, it enhanced the lymphocyte blast transformation induced by both suboptimal and optimal concentrations of concanavalin a (con a) and lens culinaris (lc). the in vivo administration of ep-1 caused a depre ... | 1984 | 6374218 |
| distinct cytoskeletal domains revealed in sperm cells. | antibodies against different cytoskeletal proteins were used to study the cytoskeletal organization of human spermatozoa. a positive staining with actin antibodies was seen in both the acrosomal cap region and the principal piece region of the tail. however, no staining was obtained with nitrobenzoxadiazol-phallacidin, suggesting that most of the actin was in the nonpolymerized form. most of the myosin immunoreactivity was confirmed to a narrow band in the neck region of spermatozoa. tubulin was ... | 1984 | 6381503 |
| dna binding proteins in canine sera. a method for removal of nonspecific dna binding in the farr assay. | a panel of canine sera, the majority of which were collected from clinically healthy dogs, were investigated for antibodies against double stranded (dsdna) by the farr radioimmunoassay technique. non-specific dna binding agents interfering with the farr assay were detected in all sera. heat inactivation at 60 degrees c or treatment with dextran sulphate was shown to eliminate this kind of unspecific dna binding while not affecting true antibodies to dsdna. canine sera positive in the farr assay ... | 1984 | 6388143 |
| d6.1, a murine antimelanoma monoclonal antibody from congenitally athymic nude mice immunized with purified melanoma tumor-associated antigen. | an igm monoclonal antibody, d6.1, has been obtained by fusion of p3 x 63ag8.653 with spleen cells from congenitally athymic nu/nu mice immunized with partially purified human melanoma taa bound to insolubilized lens culinaris lectin. the d6.1-defined antigen is present on the cell membrane of melanoma cells maintained in tissue culture but is not detected in spent culture media. eighteen of twenty-three melanoma cell lines tested were found to react with d6.1, in contrast to only 17% of nonmelan ... | 1984 | 6396196 |
| structural studies on glycoprotein oligosaccharides of chromaffin granule membranes and dopamine beta-hydroxylase. | dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. the high-mannose oligosaccharides contain an average of six mannose residues. the largest biantennary oligosaccharides (40% of the total) have two complete peripheral branches consisting of sialic acid-galactose-n-acetylglucosamine, but an equal proportion lack sialic acid on ... | 1984 | 6696440 |
| primary structure of n-glycosidically linked asialoglycans of secretory immunoglobulins a from human milk. | the asialoglycopeptides obtained from secretory immunoglobulins a from human milk have been separated by gel filtration and affinity chromatography on concanavalin a-sepharose and lens culinaris agglutinin-sepharose columns. their structures have been determined by sugar analysis, methylation studies including mass spectrometry and 500-mhz 1h-nmr spectroscopy. the glycans are of the biantennary n-acetyllactosamine type differing in their degree of extension by fucosyl-n-acetyllactosamine residue ... | 1984 | 6698017 |
| monoclonal antibodies to rat liver cell membrane glycoproteins. | monoclonal antibodies were raised against membrane glycoproteins prepared from rat liver using lentil-lectin affinity chromatography. monoclonal antibodies to rat hepatocytes with three different characteristics were obtained and designated as ham.1, ham.2 and ham.3. the binding characteristics of these three monoclonal antibodies were analysed by flow cytofluorography and indirect radioimmunoassay; ham.3 recognized a membrane antigen restricted mainly to hepatocytes. however, most rat hepatoma ... | 1984 | 6727876 |
| receptors for pea and lentil lectins on human lymphoid cells: demonstration of distinct lectin-defined cell subclasses. | lectins are useful probes for studying cell surface glycoconjugates. pea (pl) and lentil (ll) lectin each requires for binding a fucosyl- and two alpha-mannosyl residues in core regions of glycopeptides, but differences in outer chain carbohydrates may alter their relative binding affinities. we used binding studies with [125i]-pl and ll and flow cytometry with fluorescein-conjugated (fitc)-pl and -ll to study their interactions with peripheral lymphocytes. binding of both lectins to lymphocytes ... | 1984 | 6736137 |
| change in glycosylation of membrane glycoproteins after transfection of nih 3t3 with human tumor dna. | altered glycosylation of membrane glycoproteins was demonstrated in nih 3t3 cells transformed by transfection with dna from human neuroblastoma and bladder carcinoma cell lines. the oncogenes of these two cell lines have been identified as n-ras and c-h-ras-1, respectively. the fucose-labeled membrane glycopeptides of transfection-induced transformants had decreased binding to concanavalin a-sepharose when compared in dual-isotope experiments to those from nih 3t3 cells, whereas binding to lenti ... | 1984 | 6744291 |
| glycosylated ovine prolactin. | a modification of prolactin, in which the asparagine at position 31 carries a carbohydrate unit, was isolated from ovine pituitary glands. sequence and amino acid analyses identified the point of linkage of the carbohydrate. glucosamine was found in acid hydrolysates, an indication that the carbohydrate is attached through n-acetylglucosamine. the glycosylated prolactin binds to concanavalin a and lentil lectin and is eluted with methyl alpha-d-mannopyranoside. during gel electrophoresis in sodi ... | 1984 | 6582495 |
| interaction of human platelet membrane glycoproteins with collagen and lectins. | the binding of platelets to collagen is the first step in hemostasis. we attempted three approaches for elucidation of the chemical nature of receptors of human platelets for collagen. first, we examined the effect of platelet surface alteration by chymotrypsin treatment. on increasing the concentration of chymotrypsin, collagen-induced platelet aggregation and the release reaction decreased, an in parallel with this change, remarkable decrease of membrane glycoproteins iib and v, as well as 400 ... | 1984 | 6229286 |
| ige-specific suppressor factors in normal human serum. | the effect of normal human serum on in vitro ige production was studied in an attempt to determine whether ige-specific suppressor factors are present in the circulation of nonallergic individuals. sera from 10 nonatopic donors (serum ige less than 20 i.u./ml) were filtered through diaflo cf50a membranes (cutoff point 50,000 d) and various dilutions of the ige-free serum filtrates (less than 150 pg/ml of ige) were examined for their ability to suppress spontaneous in vitro ige synthesis by perip ... | 1984 | 6467688 |
| solubilization and some properties of a semicarbazide-sensitive amine oxidase in brown adipose tissue of the rat. | a semicarbazide-sensitive clorgyline-resistant amine oxidase (ssao) was solubilized from membrane fractions of rat brown adipose tissue by the non-ionic detergent, triton x-100. alteration of ionic strength or addition of chelating agents alone failed to release the enzyme from its membrane. lipid-depletion led to loss of enzyme activity and alteration of substrate affinity. over 80% of the activity of the solubilized enzyme was found in gel filtration fractions corresponding to an mr of between ... | 1984 | 6477528 |
| phagocytosis of lectin-coated beads by dystrophic and normal retinal pigment epithelium. | in the dystrophic pigmented royal college of surgeons (rcs) rat, the retinal pigment epithelium (rpe) has a diminished capacity to phagocytose shed photoreceptor outer segments (ros). an alteration in phagocytic recognition or ligand-receptor interactions between the rpe and ros's could contribute to this defect. to this end, we have examined whether or not rpe lectin receptors are implicated in phagocytosis in the normal and dystrophic rat rpe by comparing differences in phagocytic uptake of le ... | 1984 | 6479240 |
| lectin-ferritin binding on dystrophic and normal retinal pigment epithelial membranes. | phagocytosis in the rat retina is a process which involves uptake of shed photoreceptor outer segments by the overlying retinal pigment epithelium (rpe). in rats with inherited retinal degeneration, there is a defect in phagocytosis. one aspect of this phagocytic defect may be an alteration in glycoconjugate-containing membrane components on rpe membranes which mediate this phagocytic interaction. lectin binding sites have been studied in order to localize the distribution of glycoconjugates on ... | 1984 | 6481408 |
| immunosuppressive factors in ascites fluids from ovarian cancer patients. | the nonspecific immunosuppressive effect of ascites fluids from ovarian cancer patients was examined and compared with that of noncancerous abdominal effusion and sera from ovarian cancer patients. the malignant ascites fluids produced a noncytotoxic, dose-dependent suppression of dna synthesis of phytohemagglutinin-stimulated human peripheral blood lymphocytes. the suppression was higher than that observed in sera from cancer patients. no suppressive effect was seen in control abdominal effusio ... | 1984 | 6528882 |
| identification of subpopulations of human urinary plasminogen activators. | enzymes functioning as plasminogen activators in commercial urokinase preparations and individual human urine concentrates were subjected to affinity chromatography on columns of lentil lectin-sepharose, ricin-sepharose, wheat germ agglutinin-sepharose, lotus lectin-sepharose and concanavalin a-sepharose. chromatography of the enzymes from both sources yielded similar results for all lectins except lentil lectin. urokinase from several commercial sources was approximately 50% adherent to lentil ... | 1984 | 6539958 |
| flow cytometric analysis of lectin-binding to canine blood lymphocytes. | the cell surface glycoconjugates of blood lymphocytes from 19 dogs with and without neoplastic disease were quantitated using flow cytofluorometric analysis of the binding characteristics of 3 lectins, namely, wheat germ agglutinin, concanavalin-a, and lens culinaris agglutinin. the specificity of lectin binding was determined using competitive monosaccharide inhibitors. the results show enhanced binding of concanavalin-a to blood lymphocytes from dogs with lymphosarcoma relative to healthy dogs ... | 1984 | 6548849 |
| lectin-induced modulation of the antibody response to type iii pneumococcal polysaccharide. | several lectins were tested for their capacity to alter the antibody response to type iii pneumococcal polysaccharide (sss-iii). the antibody response was enhanced by concanavalin a (con a), phytohemagglutinin (pha), as well as lectins from phytolacca americana (pa-2), pisum sativum (psa), and lens culinaris (lch), when these lectins were given 2 days after immunization with sss-iii; however, suppression was obtained when con a and pa-2 were given at the time of immunization. by contrast the lec ... | 1984 | 6692477 |
| carbohydrate chain analysis by lectin binding to electrophoretically separated glycoproteins from murine b16 melanoma sublines of various metastatic properties. | cellular glycoprotein carbohydrate chains of b16 melanoma sublines of various metastatic colonization capacities were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and direct lectin staining, combined with chemical modification of carbohydrate chains in situ. for these studies, we utilized b16 sublines selected for low (b16-f1) or high lung (b16-f10), high brain (b16-b15b), or high ovary (b16-o13) colonization properties, or high tissue invasiveness in ... | 1984 | 6692378 |
| amniotic fluid analysis in prenatal diagnosis of neural tube defects: a comparison between six biochemical tests supplementary to the measurement of amniotic fluid alpha-fetoprotein. | concanavalin a (con a) and lens culinaris agglutinin (lca) microheterogeneity pattern of afp (crossed affinity immunoelectrophoresis), alpha-2-macroglobulin and synaptic membrane protein d-2 (rocket immunoelectrophoresis) and qualitative (polyacrylamide gel electrophoresis) and quantitative (enzyme kinetic reaction rate) acetylcholinesterase were analysed in 87 consequtive samples from normal pregnancies and 37 abnormal samples (fetal neural tube defect or abdominal wall defect). very few false ... | 1984 | 6209039 |
| variation in the core and branch carbohydrate sequences of serum glycoprotein hormone alpha subunit as determined by lectin affinity chromatography. | serum alpha subunits from patients with pituitary tumours and from normal controls were studied for their ability to bind to lens culinaris agglutinin-sepharose (lca), l-phytohaemagglutinin-agarose (l-pha) and soybean agglutinin-sepharose (sba). serum alpha subunits from normal controls which had previously been shown to bind to concanavalin a-sepharose (con a) were not retained by lca. in contrast, con a-reactive alpha subunits from patients with pituitary tumours bound specifically to lca. non ... | 1984 | 6207259 |
| differential reactivity of alpha-fetoprotein with lectins and evaluation of its usefulness in the diagnosis of hepatocellular carcinoma. | the reactivity of alpha-fetoprotein (afp) with concanavalin a (con a) and lens culinaris agglutinin (lca) was studied by crossed immuno-affinoelectrophoresis of the serum samples of 146 patients from three groups (groups i, ii and iii). fifty-one patients with benign liver diseases were included in group i, 83 patients with hepatocellular carcinoma in group ii, and 12 patients with carcinoma metastatic to the liver from digestive organs in group iii. in group i, the percentage of con a-reactive ... | 1984 | 6209189 |
| aspartate aminotransferase allozyme variation in a germplasm collection of the domesticated lentil (lens culinaris). | variation at a polymorphic aspartate aminotransferase locus was assayed in a sample of 298 accessions from the icarda germplasm collection of the domesticated lentil (lens culinaris). two alleles aat-1 (f) and aat-1 (s) were detected with global frequencies of 0.51 and 0.49, respectively. fifty-nine percent of accessions were polymorphic for both alleles. the frequency of outcrossing was estimated from the observed heterozygosity to be about 1%. this is higher than direct estimates of outcrossin ... | 1984 | 24257735 |
| [quantification of lectin receptors on b, t, t-gamma and t-mu lymphocytes. i. concanavalin a, pisum sativum, lens culinaris and wheat-germ agglutinin]. | the immune system is formed of different lymphocyte subpopulations, each one having a defined role to defend the organism. their plasma membranes present differences in the glycoproteinic or/and glycolipidic composition, as detected with labelled 125i-lectins. b lymphocytes have a greater number of receptors for the pisum sativum, lens culinaris and wga lectins than t lymphocytes. on the other hand, t lymphocytes bind a greater number of concanavalin a molecules than b lymphocytes. wga lectin ap ... | 1984 | 6611567 |
| superoxide dismutase from lens esculenta: purification and properties. | superoxide dismutase has been purified to homogeneity from lens esculenta cotyledons and shoots. the two forms appeared to be identical. the purified enzyme contained two electrophoretically distinct bands. it contained two ions of cu and two ions of zn. gel filtration experiments indicate a molecular weight of about 33,000. the spectrum of ultraviolet and visible regions and electron paramagnetic resonance were similar to those of cu-zn mammalian superoxide dismutase. | 1985 | 16664244 |
| platelet membrane glycoprotein abnormalities in patients with myeloproliferative disorders and secondary thrombocytosis. | carbohydrate-specific surface labelling and 125i-labelled lectin binding techniques, in combination with one- or two-dimensional (non-reduced/reduced) sds-polyacrylamide gel electrophoresis have been used with platelets from patients with myeloproliferation disorders and secondary thrombocytosis and from healthy donors. in essential thrombocythaemia platelet membrane glycoproteins were significantly less sialylated than in normals (particularly gp ib and iiia). increased binding of 125i-labelled ... | 1985 | 4005182 |
| [comparative analysis of membrane glycoproteins of normal and chronic lymphatic leukemia b lymphocytes by lectins]. | eight plant lectins were used to investigate membrane alterations in lymphocytes from patients with chronic lymphocytic leukemia (cll). by rosetting with lectins attached to latex particles, the cell percentages with the abundance of each lectin receptor were compared in b normal and leukemic lymphocytes. comparing these data with the number of lectin molecules bound to each cell and the affinity, which are values calculated with 125i-labeled lectins, it was possible to deduce differences in the ... | 1985 | 3875881 |
| partial characterization of n-butanol-solubilized rejection-type antigens of syngeneic murine colon tumors. | previous investigation of the transplantation immunity of 2 cultured murine colon lines of balb/c origin, c-c36 and c-c26, showed these tumor lines to be immunogenic against individual tumors and to have possibly cross-reactive, tumor-rejection-type antigens. for characterization of the molecular features of tumor-rejection antigens expressed on the colon tumor cells, n-butanol was used for the extraction of rejection-type antigens from tumor cells and immunogenic molecules were analyzed on tran ... | 1985 | 3857382 |
| histochemical study of epididymal secretions in the lizard, lacerta vivipara. localization of lectin-binding sites. | the epididymis of lizards elaborates voluminous secretory granules made of a central core and a peripheral vacuole which in the species lacerta vivipara contain respectively an insoluble protein (protein h) and a soluble protein (protein l). after their discharge these secretions mix with spermatozoa. in order to detect the presence of carbohydrates in these secretions, lectins isolated from canavalia ensiformis (con a) and from eleven other plants (lentil, soja, pea, gorse and several mushrooms ... | 1985 | 3884166 |
| determination of diamine oxidase in lentil seedlings by enzymic activity and immunoreactivity. | a competitive radioimmunoassay for the quantitation of diamine oxidase (ec 1.4.3.6) from lens culinaris is reported. specific antibodies raised in rabbits immunized with a homogeneous preparation of the enzyme were incubated with purified (125)i-enzyme and with either unlabeled diamine oxidase or plant material. antigen-antibody complexes were isolated from the mixture by incubation with staphylococcus protein a. the sensitivity of the test was about 5 nanograms in terms of enzyme protein. this ... | 1985 | 16664402 |
| proteoglycans from bovine nasal and articular cartilages. fractionation of the link proteins by wheat germ agglutinin affinity chromatography. | two forms of link protein, 46 and 51 kda, are present in proteoglycan aggregates from both bovine nasal and bovine articular cartilages. studies reported here show that the link proteins bind to concanavalin a, lens culinaris agglutinin, ricinus communis agglutinin, soybean agglutinin, and wheat germ agglutinin lectins. when the link proteins are eluted from these lectins with appropriate competing sugars, the 46- and the 51-kda link proteins elute together and no separation is achieved. however ... | 1985 | 3840478 |
| biosynthesis of the major human red cell sialoglycoprotein, glycophorin a. o-glycosylation. | the biosynthesis of the major human red cell sialoglycoprotein, glycophorin a, was studied in the erythroleukemia cell line k562 with emphasis on o-glycosylation. the cells were pulse-chase labeled with [35s] methionine, and either directly immune precipitated with anti-glycophorin a antiserum or detergent-solubilized extracts first passed through columns containing the n-acetylgalactosamine-specific lectin from helix pomatia or the glucose/mannose specific lectin from lentil beans. from the sug ... | 1985 | 3861610 |
| purification and characterization of a human tumor necrosis factor from the lukii cell line. | a factor with tumor necrosis factor (tnf) activity produced by the lukii human lymphoblastoid cell line [designated tnf(lukii)] was purified sequentially by using controlled-pore glass, lentil lectin-sepharose, and procion red agarose chromatography, yielding tnf with a specific activity of 1.5 x 10(7) units per mg of protein and an isoelectric point of approximately equal to 6.7. purified tnf(lukii) fractionated by nadodso4/page under reducing as well as nonreducing conditions was found to cont ... | 1985 | 3863119 |
| the large sialoglycoprotein of human lymphocytes. ii. biochemical features. | large sialoglycoprotein of human lymphocytes (l-lsgp) from thymocytes and from peripheral blood lymphocytes (pbl) of normal donor and of b chronic lymphocytic leukemia (cll) patients was purified by affinity chromatography to maclura pomifera agglutinin (mpa)-sepharose followed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). l-lsgp from the three different sources was very similar in amino acid composition. it contained a high proportion of acidic and hydroxy ... | 1985 | 3873337 |
| induction and characterization of lymphotoxins from tumor promoter-synergized, lectin-stimulated human lymphocytes in vitro. | the tumor promoters mezerein and phorbol myristate acetate, and the phorbol diesters phorbol diacetate, phorbol dibenzoate, and phorbol didecanoate synergistically enhanced the production of lymphotoxin (lt) by phytohemagglutinin-stimulated human peripheral blood or tonsil and adenoid lymphocytes. lt production was elevated 2-20-fold, depending on such parameters as the nature of the promoter and dose, the lectin dose, and the lymphocyte source. the increased lt levels were primarily due to enha ... | 1985 | 3873519 |
| the effect of h-2ij disparity on induction of allotransplantation tolerance by lentil lectin. | the effect of an h-2ij disparity on skin graft survival was studied in 18 mouse donor-recipient strain combinations, in which the recipients were treated with an efficient immunosuppressant, lentil lectin (lca). the simultaneous i-j disparity essentially had no (or a slightly adverse) effect on the graft survival times in strain combinations differing at the k and i-a loci or in the entire h-2 complex. in two strain combinations incompatible at the d locus, the simultaneous i-j disparity promote ... | 1985 | 3884497 |
| cellular cytotoxicity of trypanosoma cruzi mediated by different classes of antibody and by lectins. | we have investigated the nature of the ligand involved in antibody-dependent cellular cytotoxicity (adcc) of human leukocytes to epimastigotes of trypanosoma cruzi. purified anti-t. cruzi igg was highly efficient in mediating adcc whereas igm mediated the killing of this parasite poorly even at high concentrations. the presence of the fc portion on the igg molecule seems to be necessary since f(ab')2 derived from anti-t. cruzi igg did not mediate adcc. we also present evidence suggesting that th ... | 1985 | 3913480 |
| monoclonal antibodies prepared against the major drosophila nuclear matrix-pore complex-lamina glycoprotein bind specifically to the nuclear envelope in situ. | a high molecular weight glycoprotein found associated with a nuclear matrix-pore complex-lamina (nmpcl) preparation obtained from drosophila melanogaster embryos has been shown by in vitro analyses to be largely confined to this subcellular fraction. in contrast with several of the nmpcl proteins, this glycoprotein remains completely insoluble after treatment with 5 m urea. it has, therefore, been possible to separate the glycoprotein from other nmpcl components by differential urea extraction. ... | 1985 | 3919018 |
| analysis of the secretions of the subcommissural organs of several vertebrate species by use of fluorescent lectins. | the glycoprotein secretions of the subcommissural organ were analyzed with the use of nine fluorescent lectins, specific to different sugar moieties. after exposure to concanavalin a a bright fluorescence was observed in the ependymal cells of the subcommissural organs of all vertebrates studied (lampetra planeri, ameiurus nebulosus, bufo bufo, lacerta vivipara, gallus gallus, rattus norvegicus, ovis aries). the fluorescence is abolished by the competitive sugar, alpha-d-mannopyranosyl. the inte ... | 1985 | 3919951 |
| relationship among ige-binding factors with various molecular weights. | the rat-mouse t cell hybridoma 23b6 forms ige-binding factors on incubation with ige. the hybridoma cells incubated with ige contained intracellular ige-binding factors of 60k, 30k, 14k, and 10k daltons, and secreted the 60k, 30k, and 14k ige-binding factors. both intracellular and extracellular ige-binding factors of 60k and 14k daltons selectively suppressed the ige response, whereas the 30k and 10k factors failed to do so. reduction and alkylation of the extracellular 60k ige-binding factors ... | 1985 | 3928755 |
| structural comparisons of two allelic variants of human placental alkaline phosphatase. | a simple immunosorbent purification scheme based on monoclonal antibodies has been devised for human placental alkaline phosphatase. the two most common allelic variants, s and f, have similar amino acid compositions with identical n-terminal amino acid sequences through the first 13 residues. both variants have identical lectin binding properties towards concanavalin a, lentil-lectin, wheat germ agglutinin, phytohemagglutinin and soybean agglutinin, and identical carbohydrate contents as reveal ... | 1985 | 3934008 |
| processing of rat and human angiotensinogen precursors by microsomal membranes. | we have studied the processing of rat and human angiotensinogen precursors by microsomal membranes as a means of determining the number of asparagine-linked oligosaccharide units per angiotensinogen molecule, and thus the utilization of potential sites of n-glycosylation. glycosylated, processed forms of angiotensinogen were isolated by chromatography on lentil lectin-sepharose 4b. 35s-methionine-labeled precursor and processed forms of angiotensinogen were compared with glycosylated and nonglyc ... | 1985 | 3934016 |
| lectin characterization of gonococci from an outbreak caused by penicillin-resistant neisseria gonorrhoeae. | a total of 40 neisseria gonorrhoeae isolates, representing 19 penicillin-resistant isolates (from 8 heterosexual patients and 11 homosexual patients) and 21 penicillin-susceptible isolates (from 15 heterosexual patients and 6 homosexual patients) and obtained from the same geographic area, were examined. lectin agglutination patterns were based on the reactivity of the isolates with the following 14 lectins: concanavalin a, lens culinaris, trichosanthes kinlowii, griffonia simplicifolia i, arach ... | 1985 | 3935658 |
| involvement of cell surface macromolecules sensitive to alkylating ketones in lysis by human peripheral blood nk cells. | natural cytotoxicity (mediated by the b73.1+ subset) of human peripheral blood lymphocytes against the k-562 erythroleukaemia cell line is dramatically inhibited in a dose-dependent manner, by the small molecular weight protease inhibitors, tosyl-l-lysyl chloromethyl ketone (tlck) and tosylamide phenyl-ethyl-chloromethyl-ketone (tpck), incorporated into the cytotoxicity assay or after brief effector cell (but not target cell) pre-treatment. the alkylating ketones primarily affect post-binding ev ... | 1985 | 3971601 |
| structures of n-glycans of a ricin-resistant mutant of baby hamster kidney cells. synthesis of high-mannose and hybrid n-glycans. | the asparagine-linked glycopeptides (n-glycans) of a ricin-resistant mutant of baby hamster kidney (bhk) cells, ricr21, have been isolated and fractionated from a pronase digest of disrupted cells by concanavalin a (con a)-sepharose chromatography, ion-exchange chromatography, and lentil lectin chromatography. the structures of all the major n-glycans have been determined by 500-mhz h nmr spectroscopy. ricr21 synthesizes only hybrid and high-mannose n-glycans. all the hybrid structures contain o ... | 1985 | 3978088 |
| glycoconjugates of human sperm surface. a study with fluorescent lectin conjugates and lens culinaris agglutinin affinity chromatography. | distribution of glycocompounds in human spermatozoa was studied by using fluorescent lectin-conjugates. con a bound predominantly to acrosomal and posterior head regions whereas rca i bound to the acrosomal region of intact spermatozoa, stained in suspension. other lectins used (lca, wga, sba, pna) stained the the entire sperm surface. in airdried sperm smears binding of both con a and rca i were identical with the staining pattern obtained with living cells whereas lca, wga, sba and pna now bou ... | 1985 | 3978722 |
| purification and molecular characterization of bovine pregastric lipase. | a pregastric lipase was purified from calf pharyngeal tissues. the purification procedure was based on chromatographies on octyl-sepharose and lentil-lectin-sepharose followed by gel filtration. the final preparation, with an overall recovery of 26% of activity, gave a single protein band on dodecyl sulfate/polyacrylamide gel electrophoresis with a mr of 55000. the mr on gel filtration was 44-48000. the discrepancy may be due to the fact that pregastric lipase is a glycoprotein containing approx ... | 1985 | 3987686 |
| defective leukocyte interferon response in children with recurrent infections accompanied by arthralgia. | children with recurrent and/or unusually severe infections were investigated for possible defects in the interferon (ifn)-natural killer (nk) cell system. two series, each of 13 children, were examined, one in 1982 and one in 1983. healthy children, seven in 1982 and eight in 1983, served as controls. peripheral blood mononuclear leukocytes were examined for ifn production induced by the ifn-alpha inducers sendai virus and escherichia coli and by the ifn-gamma inducers concanavalin a and lens cu ... | 1985 | 3993368 |
| cuticular carbohydrates of three nematode species and chemoreception by trichostrongylus colubriformis. | the cuticle of haemonchus contortus, nippostrongylus brasiliensis and trichostrongylus colubriformis contained n-acetylgalactosamine and n-acetylglucosamine, based on the binding of fluorescein-labelled lectins. binding of the lectins varied between the sexes and body regions of the nematodes. treatment of male t. colubriformis with the lectin lens culinaris agglutinin (lca) reduced the feeding by helminths that was stimulated by histamine and the male's response to their female's pheromone, bas ... | 1985 | 3998961 |
| characterization of plasma-membrane glycoproteins from functional domains of the rat hepatocyte. | plasma-membrane glycoproteins from the three different functional domains of the rat hepatocyte were radioactively labelled by oxidation with naio4, followed by reduction with nab3h4. analysis of the radioactively labelled glycoproteins by polyacrylamide-gel electrophoresis revealed the presence of at least 12 major sialoglycoproteins in each different region of the hepatocyte surface. the mr-110 000 component was homogeneously distributed over the plasma membrane, whereas the mr-90 000 polypept ... | 1985 | 4004780 |
| structural features of the autoantigens involved in thyroid autoimmune disease: the thyroid microsomal/microvillar antigen. | the microsomal/microvillar antigen of the human thyroid gland which provokes thyroid autoimmunity was characterised by immunoprecipitation studies. sera from patients with hashimoto's thyroiditis, primary myxoedema or graves' disease containing autoanti-microsomal antibody specifically precipitated a component, which under reducing conditions migrates with a mol. wt of 105,000 on sds-polyacrylamide gel electrophoresis. this protein was absent in auto- or xeno-anti-thyroglobulin precipitates, whi ... | 1985 | 4022016 |
| identification of a tumor cell-derived differentiation antigen on mouse colony-forming units in the spleen and progenitor cells. | surface membrane antigen(s) expressed on a mouse mast cell line (fmp1) have also been shown to occur on hemopoietic spleen colony-forming units (cfu-s) and granulocyte/macrophage colony- and erythroid burst-forming cells, using a xeno-antiserum raised against fmp1 cells. this mast cell model has been used to obtain antiserum and large quantities of antigen for the biochemical identification of cfu-s and progenitor cell antigen(s). immunoprecipitation of fmp1 membrane antigens with the antiserum ... | 1985 | 4027983 |
| a human sperm coating antigen isolated from sperm cell membrane. | a human sperm cell membrane antigen that is highly specific to sperm and seminal plasma was isolated from plasma membrane fraction of spermatozoa using rabbit antiserum against human seminal plasma. in addition to the high specificity to sperm and seminal plasma, the isolated antigen showed the following characteristics: (1) it is a glycoprotein of approximately 12,000 daltons that has an affinity to lentil lectin; (2) it is distributed in human milk other than in sperm and seminal plasma, but i ... | 1985 | 4037175 |
| alterations in fucosyl oligosaccharides of glycoproteins during rat liver regeneration. | [3h]fucose-labelled glycopeptides in the slices of liver 24h after partial hepatectomy were fractionated on sephadex g-50. glycopeptides from regenerating liver contained a higher proportion of lower-mr components than did controls. regenerating liver contained a higher proportion of glycopeptides that were bound to concanavalin a-sepharose and were subsequently eluted with 20mm-methyl alpha-d-glucopyranoside than did controls. concanavalin a-bound glycopeptides from each source were entirely bo ... | 1985 | 4038278 |
| purification and characterization of a high molecular weight glycoprotein detectable in human milk and breast carcinomas. | the murine monoclonal antibody (mab) designated df3 has defined a high m.w. antigen detectable in human breast carcinomas and in human milk. df3 antigen is detectable on apical borders of secretory mammary epithelial cells and in the cytosol of less differentiated malignant cells. df3 antigen expression has been shown to correlate with the degree of human breast tumor differentiation, and the detection of a cross-reactive species in human milk has suggested that df3 antigen might be useful as a ... | 1985 | 4045199 |
| biochemical studies on the 30-40 kda schistosoma mansoni surface antigens. | biochemical studies of the previously identified 30-40 kda surface antigens of schistosoma mansoni schistosomula confirmed that four molecules could be discriminated in this antigenic group. the antigens presented slightly different molecular mass in sodium dodecyl sulfate polyacrylamide gel electrophoresis (40, 38, 37 and 32 kda) but were all found in isoelectric focusing at the same ph (6.2-6 and 7.5). the four antigens bound to concanavalin a and only the 32 kda molecule had affinity for the ... | 1985 | 4058484 |
| effects of feeding ascochyta-infected and normal lentils to rats (short-term study). | weanling, female, sprague-dawley rats were fed diets containing either rat chow with no lentils, 80% normal lentils or 80% diseased lentils heavily infected with the fungus ascochyta lentis. body weight, feed consumption and clinical appearance were monitored over 90 days and blood samples were collected at the termination of the experiment. weight gain and feed consumption were similar in the control group and the group fed diseased lentils. weight gain was slightly depressed in the group fed n ... | 1985 | 4075241 |
| the interaction of hydralazine with a semicarbazide-sensitive amine oxidase in brown adipose tissue of the rat. its use as a radioactive ligand for the enzyme. | hydralazine is a potent irreversible inhibitor of the semicarbazide-sensitive amine oxidase (ssao) found in brown fat. initially it may act on the enzyme as a competitive inhibitor, but irreversible inhibition occurs rapidly in a concentration- and temperature-dependent manner. the presence of primary amines known to be substrates for the enzyme, but not of secondary amines, which are not metabolized by ssao, diminishes this rate of inactivation, whereas removal of o2 is without effect. the kine ... | 1985 | 4091799 |
| further resolution of human alpha-fetoprotein by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin. | major molecular species of human alpha-fetoprotein(afp), which were separated as single components by serial affinity chromatography with concanavalin a(con-a) and lens culinaris agglutinin, were further resolved into several bands by affinity electrophoresis with erythroagglutinating phytohemagglutinin of phaseolus vulgaris lectin(e-pha). among the newly separated main molecular species, both con-a- and e-pha-reactive afp(afp-1x1) was demonstrated, contrary to the known sugar specificity of con ... | 1985 | 2426755 |
| presence of an antigen-specific t cell subset that forms ige-suppressive factor and igg-suppressive factor on antigenic stimulation. | b6d2f1 mice were given three i.v. injections of ovalbumin (oa), and antigen-specific t cell clones were established from their spleen cells. one of the fcr+ t cell clones formed ige-binding factors on incubation with oa-pulsed syngeneic macrophages. neither soluble antigen nor macrophages alone induced factor formation. t cell hybridomas were constructed by fusion of the antigen-specific t cell clone with bw 5147 cells. among 11 t cell hybridomas established, six clones produced ige-binding fact ... | 1985 | 2409160 |
| transneuronal transport of lectins. | axonal and transneuronal transport of the plant lectins wheat germ agglutinin (wga), pisum sativum agglutinin (psa), lens culinaris agglutinin (lca), soybean agglutinin (sba), peanut agglutinin (pna), concanavalin a agglutinin (con a), and ulex europeus agglutinin (uea) were examined and compared using an immunocytochemical staining method. wga, which binds to n-acetylglucosamine and sialic acid carbohydrate residues, and the 3 mannose binding lectins (con a, psa and lca) were found to undergo r ... | 1985 | 2412649 |
| alpha-fetoprotein subfractions in amniotic fluid identified by a modification of the method of concanavalin a, lentil lectin or phytohemagglutinin-e affinity crossed-line immunoelectrophoresis. | using a modified method of concanavalin a (con a), lentil lectin (lch) or phytohemagglutinin-e (pha-e) affinity crossed-line immunoelectrophoresis, alpha-fetoprotein (afp) subfractions were studied in 33 samples of human amniotic fluid obtained between 41 and 287 days of gestation. fetal tissues (yolk sac, liver, stomach and small intestine) obtained from a fetus of 68 days' gestation were incubated for 24 hours and afp subfractions in the culture fluid examined. afp in control amniotic fluids y ... | 1985 | 2416031 |
| glycosylated human prolactin. | a glycosylated form of human prl (g-hprl) was isolated from pituitary glands. the glycoprotein was separated from the major form of prl on columns of lentil lectin-sepharose 4b. the major form of prl did not bind to the lentil lectin, whereas the glycosylated modification did and could be eluted with methyl-alpha-d-mannopyranoside. by gel electrophoresis in sodium dodecyl sulfate, a mol wt of 25,000 was estimated for the glycosylated prl. the mol wt of hprl is 23,000. in a ria for hprl, the glyc ... | 1985 | 2578035 |
| natural killer (nk) cell activating factor released from murine thymocytes stimulated with an anti-tumor streptococcal preparation, ok-432. | natural killer (nk) activity of mouse splenocytes was significantly augmented when the splenocytes were incubated for 3 to 4 hr with culture supernatants of mouse thymocytes stimulated by ok-432, an antitumor preparation from the streptococcus pyogenes su-strain. antiviral activity was also detected in the culture supernatants, but il 2 activity was not. when the culture supernatants of thymocytes stimulated by ok-432 were fractionated on a column of blue sepharose cl-6b, nk enhancing activity a ... | 1985 | 2578148 |
| a glycoprotein of molecular weight 85,000 on human cells of b-lineage: detection with a family of monoclonal antibodies. | immunization of balb/c mice with glycoproteins purified from a detergent extract of human chronic lymphocytic leukemia (cll) cells by affinity to lens culinaris lectin led to the production of several monoclonal antibodies with similar reactivity. one of the antibodies, 50b4, was purified and the corresponding antigen was isolated from a b-lymphoblastoid cell line extract by affinity chromatography to the 50b4-igg immunoadsorbent. co-purification of the antigenic activities associated with five ... | 1985 | 2579328 |
| purification and properties of human placental dipeptidyl peptidase iv. | dipeptidyl peptidase iv (ec 3.4.14.5; gly-pro-p-nitroanilidase) was purified 1840-fold from human placenta and characterized. the enzyme was solubilized from membrane fractions with triton x-100 and subjected to zinc acetate fractionation, hydroxylapatite chromatography, sephacryl s-300 chromatography and then affinity chromatographies with lentil lectin-sepharose 4b and gly-pro-nh-(ch2)6-nh-sepharose 4b. dipeptidyl peptidase iv was completely separated from leucine aminopeptidase by the final a ... | 1985 | 2860191 |
| characterization of gamma-glutamyltranspeptidase from human exocrine pancreatic carcinomas. | two different types of gamma-glutamyltranspeptidase (gamma-gtp) have been found in normal human pancreas following bromelain treatment. on the other hand, three human pancreatic ductal cell carcinomas have only a single type of gamma-gtp upon analysis with polyacrylamide gel electrophoresis, anion-exchange column chromatography and isoelectric focusing. carcinoma gamma-gtps were almost identical to one of the two types of normal pancreatic gamma-gtps. the gamma-gtp from pancreatic carcinomas bou ... | 1985 | 2863014 |
| isolation and characterization of thy 1 homologue from human thymus. | a 40 000 m.w. glycoprotein was isolated from human thymus. this molecule binds lentil lectin, reacts with an antiserum made against the p25 antigen (the human thy 1 homologue) and possesses almost identical amino acid composition as the p25 antigen and its 40 000 m.w. dimer. | 1985 | 2864756 |
| lentil lectin binding properties of the leucocyte common antigen from rat dividing and non-dividing thymocytes. | the surface glycoproteins of rat large, mainly dividing, thymocytes and their small, non-dividing, progeny have been examined by labelling with 3h-sodium borohydride. a prominent glycoprotein component of apparent mol. wt. 150 k was labelled on both the cell types and by immunoprecipitation with a specific monoclonal antibody, was demonstrated to contain the leucocyte common antigen (lca). the binding of this glycoprotein to lens culinaris lectin (lch) has been studied. preliminary results obtai ... | 1985 | 2867624 |
| isolation of a subpopulation of glycoprotein iib-iii from platelet membranes that is bound to membrane actin. | triton x-100-insoluble residues, or skeletons, of plasma membrane-rich vesicles obtained from unstimulated human platelets were isolated by high speed centrifugation. about 10-15% of the total surface iodinatable glycoproteins iib and iii (gpiib and gpiii, respectively) co-isolated with the insoluble fraction. after sonication and centrifugation the solubilized material was further purified by affinity chromatography on lens culinaris lectin-sepharose. sds page analysis of this material revealed ... | 1985 | 3155751 |
| galactose-rich glycoproteins are on the cell surface of herpes virus-infected cells. 1. surface labeling and serial lectin binding studies of asn-linked oligosaccharides of glycoprotein gc. | cell-surface glycoproteins of mock-infected and herpes simplex virus type 1 (hsv-1)-infected bhk-21 and hep-2 cells were radiolabeled by incubation with galactose oxidase followed by reduction with nab3h4. the incorporation of radiolabel into glycoconjugates in both bhk-21 and hep-2 cells was increased several fold following infection with hsv, showing an increase in surface-exposed gal residues in the infected cells. this was further confirmed by an increase in binding of cell-surface-labeled g ... | 1985 | 2982317 |
| redistribution of membrane-bound and cytosolic action in rabbit polymorphonuclear leucocytes during phagocytosis. | in the analysis of highly purified surface membrane from both resting and phagocytosing neutrophils an increase in the surface membrane associated actin has been demonstrated. this change at the cell periphery is associated with a coincident increase in the f-actin content of the cells following stimulation of the cells by exposure to opsonized oil red o droplets. the actin which is newly associated with the surface membrane of the phagocytosing cells was more susceptible to removal by detergent ... | 1985 | 2983683 |
| isolation of canine neutrophil plasma membranes. | this paper describes the isolation of plasma membrane vesicles formed by nitrogen cavitation of canine neutrophils. plasma membranes from disrupted cells were separated from other membranes and organelles by percoll-density gradient centrifugation. transmission electron microscopic examination of membrane preparations chromatographed on either sephacryl s-1000 or sepharose 4b revealed that two populations of plasma membrane vesicles were formed: large (176 +/- 22nm), and small (119 +/- 11nm). pu ... | 1985 | 2993461 |
| ileal loss of available carbohydrate in man: comparison of a breath hydrogen method with direct measurement using a human ileostomy model. | the breath hydrogen technique has suggested that a considerable amount of available plus unavailable carbohydrate enters the large intestine after the consumption of starchy foods (white bread 11%, wholemeal bread 8%, and red lentils 18%). direct measurement of the available carbohydrate in ileal effluent after the consumption of test meals by three individuals with ileostomies gave values similar to those determined by the breath hydrogen technique (white bread 10%, wholemeal bread 8%, and red ... | 1986 | 3004195 |
| molecular characterization of the egf receptor and involvement of glycosyl moieties in the binding of egf to its receptor on a clonal osteosarcoma cell line, umr 106-06. | the epidermal growth factor receptor in cells of the umr 106-06 clonal osteoblast line has been shown to be structurally similar to that previously characterized in other cell lines. a specific receptor component of approximately 165,000-185,000 mr has been identified by polyacrylamide gel electrophoresis using the chemical crosslinker disuccinimidyl suberate to crosslink 125i-egf to its receptor. tunicamycin treatment of cells resulted in a dose-dependent loss of binding suggesting involvement ... | 1986 | 3006887 |
| lectin histochemistry applied to human nerves. | histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ... | 1986 | 3015069 |
| affinity to lectin, biological and immunological characteristics of human chorionic gonadotropins from pregnant women and trophoblastic tumour patients. | to compare the affinity to lectin of human chorionic gonadotropins (hcg) from pregnant women and trophoblastic tumour patients, a small amount of urine was fractionated by a lentil lectin (lch) affinity chromatography. the lch-bound fractions were eluted with 2% mannoside solution, and each fraction was assayed for hcg activities by radioimmunoassay. in pregnant women, more than 90% of hcg immunoactivity in urine was bound to the lch column and eluted from it, whereas 8 to 26% and 37 to 51% of t ... | 1986 | 3019056 |
| effects of different proportions of carbohydrates, polysaccharides/monosaccharides, and different fibers on the metabolic control in diabetic rats. | recent findings have suggested that diets with a high level of carbohydrates may impair the metabolic control of diabetes mellitus in humans. moreover, other investigations have indicated that if the simple sugar content is increased in order to attain a proportion of polysaccharides/monosaccharides equal to 1, then neither the blood glucose nor the lipidic response show any change. we have studied the effect of increasing carbohydrates in the diet (59% v 82%), while maintaining cereal fiber lev ... | 1986 | 3020344 |
| partial characterization of chicken thy-1 glycoprotein by monoclonal antibodies. | a panel of monoclonal antibodies has been raised to thy-1 purified from chicken brain. they were produced by immunization of balb/c mice with a thy-1-enriched (sephacryl b) fraction of a lentil lectin-positive fraction from solubilized brain membrane proteins. antibody-secreting clones were found to be specific for thy-1 by enzyme-linked immunosorbent assay (elisa) and western transfer and immunoblotting. four of the monoclonal antibodies were of the igg2b isotype, one was igg1, and one was igm. ... | 1986 | 2877095 |
| purification and characterization of human placental microsomal aminopeptidase: immunological difference between placental microsomal aminopeptidase and pregnancy serum cystyl-aminopeptidase. | human placental microsomal aminopeptidase (microsomal pap) was purified 3,880-fold from human placenta and characterized. the enzyme was solubilized from membrane fractions with triton x-100 and also trypsin digestion, and subjected to zinc sulfate fractionation, chromatographies with de-52, hydroxylapatite, sephacryl s-300 and lentil lectin-sepharose 4b, and finally affinity chromatography with bestatin-sepharose 4b. microsomal pap was separated from aminopeptidase a (aap) by affinity chromatog ... | 1986 | 2877870 |
| characterization of covalently cross-linked pancreatic somatostatin receptors. | the receptor for somatostatin present in rat pancreatic plasma membranes was characterized by affinity labeling with [125i-tyr11]somatostatin utilizing three different heterobifunctional cross-linking agents: n-5-azido-2-nitrobenzoyloxy-succinimide, n-succinimidyl 6-(4-azido 2'-nitrophenylamine)hexanoate, and n-hydroxysuccinimidyl 4-azido-benzoate. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography revealed a broad band of mr = 92,000 when any of the three ... | 1986 | 2877990 |
| regulatory effects of human ige-binding factors on the ige response of rat lymphocytes. | normal human peripheral blood t cells were propagated in the presence of human interleukin 2, and activated cells were incubated with human ige-dimer to induce ige binding factor formation. the cells were then fused with a mutant of the human t cell line cem. five of the t cell hybridomas formed ige binding factors upon incubation with human ige-dimer. because ige binding factors formed by the human t cell hybridomas had affinity not only for human ige but also for rat ige, the biologic activiti ... | 1986 | 3079805 |
| monoclonal antibody affinity purification of a leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding. | specific monoclonal antibody coupled to affi-gel 10 was used to purify a major membrane glycoprotein of leishmania mexicana amazonensis, one of a group of parasitic protozoa that specifically infect mammalian macrophages. immobilized antigen was eluted at a 34% efficiency with buffers at either ph 2.5 or 11 or with mgcl2, but only the antigen eluted under basic conditions could be readsorbed to the immunobeads. sephacryl s-300 gel filtration of the purified antigen gave a single peak of protein ... | 1986 | 3079902 |
| inhibition of mitogenesis induced by phytohemagglutinin and lens culinaris lectin in adherent-cell supernatants treated with protein extract of mycobacterium tuberculosis. | specific stimulation of t cells by phytohemagglutinin and lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (cfpe) derived from bacterial cultures of mycobacterium tuberculosis h37ra (avirulent) and h37rv (virulent). the induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to h37rv or h37ra cfpe. the inhibitory factor did not seem to be a cfpe reprocessed by the adherent ... | 1986 | 3082760 |
| chemical induction of sister-chromatid exchanges in human lymphocytes treated in g0 prior to stimulation by different mitogens and revealed 72 h later in second division cells. | frequencies of sister-chromatid exchanges (sce) were determined in second-division metaphases of human lymphocytes, exposed for 1 h during the g0 phase to mitomycin c (mmc) alone or to cyclophosphamide (cp) in the presence of s9 mix. the cells were then cultured for 72 h in the presence of phytohemagglutinin (pha), concanavalin a (con a), wistaria floribunda (wfa) or lens culinaris (lch-a) extracts. large differences in mitotic indices (mi) and cell-cycle kinetics were observed among cells subje ... | 1986 | 3084967 |
| interaction of trypanosoma cruzi with macrophages: effect of previous incubation of the parasites or the host cells with lectins. | the effect of incubation with lectins of the macrophages or two evolutive stages of trypanosoma cruzi (noninfective epimastigotes and infective trypomastigotes) on the ingestion of the parasites by mouse peritoneal macrophages was studied. lectins which bind to residues of mannose (lens culinaris, lca), n-acetyl-d-glucosamine or n-acetylneuraminic acid (triticum vulgaris, wga), beta-d-galactose (ricinus communis, rca), n-acetyl-d-galactosamine (phaseolus vulgaris, pha; dolichos biflorus, dba; an ... | 1986 | 3085365 |
| primary structure of a new tetraantennary glycan of the n-acetyllactosaminic type isolated from human factor viii/von willebrand factor. | n-glycosidically linked glycopeptides released by mild alkaline treatment of human factor viii/von willebrand factor (fviii/vwf) were fractionated by serial affinity chromatography on columns of sepharose linked to concanavalin a (cona) and lens culinaris agglutinin (lca). the fraction which is not retained on cona-sepharose, but eluted from lca-sepharose contains a pure minor glycopeptide which was structurally analysed. based on the results of methylation analysis and 500-mhz 1h-nmr spectrosco ... | 1986 | 3089784 |
| lectin-binding sites in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa. | the carbohydrate structures of cellular glycoconjugates in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa were analysed with six fluorescein isothiocyanate-conjugated lectins. in normal, hyperplastic and adenomatous colorectal mucosa showing mild or moderate dysplasia concanavalin a (con a), lens culinaris (lca), and wheat germ (wga) agglutinins stained goblet cell glycoconjugates (actual mucin goblet itself) while peanut (pna), vicia villosa (vva), and griffonia sim ... | 1986 | 3092568 |
| the human growth hormone receptor of cultured human lymphocytes. structural characteristics and glycosylation properties. | the structural characteristics and glycoprotein nature of the human growth hormone (hgh) receptor in cultured lymphocytes (im-9 cell line) were studied with the use of a bifunctional reagent (disuccinimidyl suberate) to couple 125i-hgh covalently to intact cells. after cross-linking, the hormone-receptor complexes were analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. a single band of mr 140,000 was identified under reducing conditions. the labelling of this band was blocke ... | 1986 | 3099769 |
| characterization of the human glomerular c3 receptor as the c3b/c4b complement type one (cr1) receptor. | the functional and immunochemical characteristics of the human glomerular c3 receptor were investigated by adherence of sheep erythrocytes (es) coated with defined c3 fragments and by using polyclonal and/or monoclonal antibodies directed against epitopes expressed on complement receptors cr1, cr2, and cr3. c3b-bearing es (esc3b) strongly adhered to glomeruli in frozen kidney sections in a reaction that was selectively inhibited by f(ab')2 anti-cr1 antibodies. there was no adherence of esc3dg, e ... | 1986 | 2418113 |
| biochemical and immunologic characterization of a major surface antigen of dirofilaria immitis infective larvae. | a 35 kd major surface antigen of dirofilaria immitis third-stage larvae was characterized biochemically and immunologically. living larvae were iodinated by using iodo-gen, iodosulfanilic acid, lactoperoxidase-glucose oxidase, and bolton-hunter reagents. detergent extracts of larvae labeled by the first three methods showed one major 35 kd component and a number of smaller components of about 6 kd, as analyzed by one-dimensional sds-page. in contrast, extracts from larvae labeled with the bolton ... | 1986 | 2419445 |
| localization of lectin binding sites in human, cat, and rabbit corneas. | paraffin sections of human, cat, and rabbit corneas were stained with nine lectins, using an avidin-biotin-complex procedure to study glycoconjugates of the epithelium, keratocytes, and stromal matrix. wheat germ agglutinin (wga) stained plasma membranes of all epithelial cell layers of cat and human and superficial and wing cells of rabbit. plasma membranes of superficial and wing cells of cat epithelium also stained with peanut agglutinin (pna) and ricinus communis agglutinin i (rca-i). human ... | 1986 | 2426217 |
| domain-specific distribution of carbohydrates in human fibronectins and the transformation-dependent translocation of branched type 2 chain defined by monoclonal antibody c6. | fibronectins purified from human plasma (termed pfn), spent culture media of human fibroblasts wi38 (termed cfn), and sv40 virus-transformed wi38/va13 cells (termed tfn) and their cleavage fragments were compared with respect to their binding activities to lectins and anti-carbohydrate antibodies reacting with chemically well-defined structures. the following findings were of particular interest. about 25-35% of cfn and tfn carried a binary sialosyl type 2 chain (neuac alpha 2----3/or 6gal beta ... | 1986 | 2426270 |
| [differential diagnosis of benign liver diseases from primary liver cancer with elevation of afp shown by lentil lectin immunoelectrophoresis]. | 1986 | 2427282 | |
| structural implications of the location and stability to proteolytic enzymes of immunodominant determinants of the human leukocyte common molecule. | in this report, we use 8 different mouse monoclonal antibodies to define 3 immunodominant determinants of the human leukocyte common (lc) antigen, and by defining the relative location and the stability to proteolytic enzymes of these determinants we make some structural predictions about the lc family of molecules. one lineage-restricted determinant is expressed predominantly on b lymphocytes and is located on the two higher (210 and 195) kda bands of lc, with possibly some expression on the 18 ... | 1986 | 2427343 |