Publications

the central cervical nucleus - a source of spinocerebellar fibres, demonstrated by retrograde transport of horseradish peroxidase.	injections of horseradish peroxidase in the cerebellum of kittens resulted in labelling of cells in the central cervical nucleus (ccn) in the upper cervical spinal cord. the largest number of labelled ccn neurones was found after injections including the anterior part of the anterior lobe of the cerebellum. in this neuronal system horseradish peroxidase was transported at a maximal velocity of about 150 mm/day.	1975	19604757
preparation and properties of matrix-supported horseradish peroxidase.	a new method of enzyme immobilization has been described using poly(4-methacryloxybenzoic acid) as the carrier. activation of the polymer, prior to enzyme attachment, was achieved with n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. the enzyme coupling step proceeded through nucleophilic attack by the protein on a mixed carbonic anhydride. the degree of polymer activation was determined by analysis for quinoline, a by-product of the reaction. the polymer-enzyme complex was compared to the enzyme ...	1975	241471
ultrastructural localization of in vivo-bound complement in bullous pemphigoid.	antihuman complement component c3 labeled with horseradish peroxidase was used to reveal the ultrastructural localization of complement in two cases of bullous pemphigoid. the complement deposits were shown to be exclusively located in the space between the plasma membrane of the basal cells and the basal lamina. this corresponds exactly to the ultrastructural localization of immunoglobulins in bullous pemphigoid.	1975	50388
an efferent connection of the solitary cells of meynert. a study with horseradish peroxidase in the marmoset callithrix.		1975	809097
localization of alpha-d-glucosyl and alpha-d-mannosyl groups of mucosubstances with concanavalin a and horseradish peroxidase.	concanavalin a is a lectin which is known to bind specifically to alpha-d-glucosyl and alpha-d-mannosyl groups in the mucosubstances of mammalian tissues. the lectin molecule is bivalent; after its attachment to mucosubstances present in a histological specimen it can also bind horseradish peroxidase, a mannose-containing glycoprotein. the attached peroxidase may then be visualized by virtue of its histochemically demonstrable enzymatic activity. other investigators have utilized this principle ...	1975	52638
photochemical reactions of horseradish peroxidase compounds i and ii at room temperature and 13 degrees k.	some photochemical reactions of horseradish peroxidase compounds i and ii (hrp-i and hrp-ii, respectively) have been studied by electronic absorption spectroscopy over the temperature range 297 degrees k-10 degrees k. in glassy matrices below 80 degrees k hrp-i is rapidly converted to hrp-ii when irradiated with low power white light. the native enzyme and hrp-ii are not photochemically active at these temperatures with low power irradiation. at room temperature the spontaneous decay of both hrp ...	1975	1148197
effects of 2,4-substituents of deuteropheme upon redox potentials of horseradish peroxidases.		1975	239639
epr studies on compound i of horseradish peroxidase.	compound i of horseradish peroxidase (donor: hydrogen-peroxide oxidoreductase ec 1.11.1.7) was studied by epr at low temperatures. an asymmetric signal was found, about 15 gauss wide and with a g-value of 1.995, which could be detected only at temperatures below 20 k and which had an intensity corresponding to about 1% of the heme content. in a titration with h2o2, the signal intensity was proportional to the concentration of compound i, reaching a maximum when equivalent amounts of h2o2 were ad ...	1975	167831
distribution of ultrastructural tracers in crustacean axons.	ruthenium red and horseradish peroxidase were used to compare the uptake of exogenous molecules into crayfish motor axons and their sheaths in severed and intact peripheral nerves. both tracers penetrated the axonal sheath and were subsequently seen lining small vesicles and tubules in the axoplasm. tracer appeared to enter the axon via pinocytotic vesicles. there were no perceptible quantitative or qualitative differences in ruthenium red uptake between intact and severed axons. however, counts ...	1975	1133229
the ultrastructure of normal and glycerol treated muscle in the ghost crab, ocypode cursor.	the ultrastructure of normal and glycerol treated fibers of the closer muscle of the ghost crab, ocypode cursor, was studiedmthe muscle is composed of presumably phasic (short sarcomeres) and tonic (long sarcomeres) fibers, the latter greatly predominating. horseradish peroxidase (hrp) was used as an extracellular tracer to delineate the tubular system (ts), and to determine to what extent this system becomes detached from the extracellular space as a result of glycerol treatment. sarcolemmal cl ...	1975	1149103
titration study of guaiacol oxidation by horseradish peroxidase.	titration of guaiacol by hydrogen peroxide in the presence of a catalytic amount of horseradish peroxidase shows that the reduction of hydrogen peroxide proceeds by the abstraction of two electrons from a guaiacol molecule. in the same way, it can be demonstrated that 0.5 mol of guaiacol can reduce, at low temperature, 1 mol of peroxidase compound i to compound ii. moreover, the reaction between equal amounts of compound i and guaiacol at low temperature produces the native enzyme. a reaction sc ...	1975	1139404
the results of freezing and dehydration of horseradish peroxidase.		1975	1132241
enzymatic activation and trapping of luminol-substituted peptides and proteins. a possible means of amplifying the cytotoxicity of anti-tumor antibodies.	glutathione and glucose oxidase (ec 1.1.3.4) conjugates containing covalently bound luminol were prepared as prototypes for peptides and proteins with latent, enzyme-activatable chemical reactivity. in the presence of small quantities of activated horseradish peroxidase, conjugated luminol molecules were oxidized to unstable free radicals which reacted rapidly with soluble proteins and cells. these observations are of interest in regard to possible sequential localization reactions in which a fe ...	1975	47175
nuclear magnetic resonance studies of hemoproteins. binding of aromatic donor molecules to horseradish peroxidase.		1975	429320
retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat.		1975	19604744
stimulation-induced uptake of horseradish peroxidase by rat cortical synapses.		1975	19604745
labelling of cells in the medulla oblongata and the spinal cord of the monkey after injections of horseradish peroxidase in the thalamus.	injections of horseradish peroxidase were performed in macaque monkeys in the intralaminar and the ventroposterior thalamic region, respectively. following injection in the intralaminar region labelled cells were present in the marginal zone both in the spinal trigeminal nuclues and in the spinal dorsal horn. the same areas contained labelled cells after injections in the ventroposterior thalamic region, but in such experiments labelled cells were also present in the lamina iv-v region of the sp ...	1975	19604756
the reaction of coumarins with horseradish peroxidase.	the peroxidase catalyzed oxidation of indole-3-acetate is inhibited by naturally occurring coumarins such as scopoletin. this inhibition is due to the preferential reactivity of the coumarins with the peroxidase compounds i, ii, and iii. in view of the possible growth regulatory role of coumarins in plants, the mechanism of oxidation of scopoletin by horse-radish peroxidase has been investigated.peroxidase catalyzed coumarin oxidation requires either an electron donor and molecular oxygen or hyd ...	1975	16659024
the isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish.	the relationship of indoleacetic acid oxidase activity to peroxidase activity is complicated by numerous multiple forms of this enzyme system. it is not known if all isozymes of this complex system contain both types of activity. isozyme analysis of commercial horseradish peroxidase and leaf extracts of yellow birch (betula alleghaniensis) by isoelectric focusing in polyacrylamide gels was used to examine this problem. horseradish and birch exhibited 20 and 13 peroxidase isozymes, respectively, ...	1975	16659371
in vitro studies on the selective binding of igg from different species to tissue sections of the bovine mammary gland.	tissue sections of the mammary gland of cattle provide a sensitive and reproducible model for studying the initial events of the selective transport of bovine iggs by the acinar epithelium (ae). mammary tissue was reacted with purified antibody to horseradish peroxidase (hrp) and exposed to hrp. the sites of peroxidase activity were revealed cytochemically. the highly selective binding of iggs by ae of the colostrum-forming gland but never of the lactating gland was most notable, whereas igg1 an ...	1975	11993319
junctional complexes in the preimplantation rabbit embryo.	the morphology and development of junctional complexes between blastomeres of the preimplantation rabbit embryo were investigated using several approaches. electron microscopic examination of embryos stained en bloc with uranyl acetate, and the study of junction permeability using horseradish peroxidase and lanthanum nitrate provided information on structure, intermembrane spacing and permeability of the junctional complexes. in addition, the freeze fracture technique was used with day 5 and day ...	1975	45878
localization of immunoreactive androgen in testicular tissue.	immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. the bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. the highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferou ...	1975	45910
use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy.	staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. the topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. the technique is therefore a useful aid to the study of cultured cells with phase opti ...	1975	46631
genetic control of sensitization to structurally unrelated antigens and its relationship to histocompatibility antigens in guinea-pigs.	five strains of guinea-pigs homozygous for gpl-a and 2/13 histocompatibility antigens were sequentially immunized with seven structurally unrelated antigens (tgal, bpo-bgg, asan, bpo-pll, phenetidine, horseradish peroxidase and dnp-dodecapeptide). considerable differences in the immune response were observed, as measured by delayed skin reactivity and antigen-induced lymphocyte stimulation in vitro. the data presented show that when the progeny of various back-cross matings are analysed for thei ...	1975	47308
the retrograde transport method.	after an injection of horseradish peroxidase into the brain, the enzyme product can later be found in cell bodies of neurons which project axons to the injection site. the use of the tracer has now been adapted as a means of identifying neurons on the basis of their efferent fiber projections. in this review particular attention is paid to characteristics of the phenomenon of retrograde transport, such as diffusion of the marker from the injection site and the region of effective uptake, uptake ...	1975	48482
retrograde axonal transport of horseradish peroxidase in peripheral autonomic nerves.	an exogeneous marker protein, horseradish peroxidase (hrp) was used to race peripheral autonomic pathways in adult guinea pigs and cats. small doses of hrp were injected into various organs and after a brief survival period, hrp activity appeared in the perikarya of autonomic neurons that supplied each injection site. after injection of hrp into the anterior chamber of the eye, reaction product was detected in the postganglionic sympathetic neurons of the superior cervical sympathetic ganglion. ...	1975	48519
hepatic nucleases. extrahepatic origin and association of neutral liver ribonuclease with lysosomes.	in the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by triton wr-1339 accumulation in lysosomes. particulate neutral ribonuclease is latent; the enzyme is unmasked by very low digitonin concentrations or hypoosmotic shock. these observations demonstrate that the bulk of liver neutral ribonuclease is associated with the lysosomal system. in view of ...	1975	1273
caveola--vesicle complexes in the plasmalemma of erythrocytes infected by plasmodium vivax and p cynomolgi. unique structures related to schüffner's dots.	erythrocytes infected with plasmodium vivax and p cynomolgi, characterized by schüffner's dots on giemsa-stained thin films, were studied by electron microscopy and immunocytochemistry. a caveola-vesicle complex, which consisted of a caveola surrounded by vesicles, in an alveolar fashion, formed along the erythrocyte plasmalemma. horseradish-peroxidase-labeled immunoglobulin from a monkey infected with p vivax bound to the vesicle membrane. cationized ferritin appeared within the vesicles after ...	1975	50017
the identification of striatal and pallidal neurons projecting to substantia nigra. an experimental study by means of retrograde axonal transport of horseradish peroxidase.		1975	51667
purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization.	mouse alpha1-fetoprotein (afp) was isolated from amniotic fluid by immunoadsorbent columns and preparative electrophoresis. specific antibodies were isolated from monospecific hyperimmunsera by use of immunoadsorbents, and subsequently coupled with horseradish peroxidase. at the light microscopical level, purified antibody-peroxidase conjugates were used for the cellular localization of afp in fetal liver by direct and indirect staining methods. fixatives containing ethanol or aldehydes were tri ...	1975	49343
demonstration of nigrothalamic connections in the cat by retrograde axonal transport of horseradish peroxidase.		1975	49210
the origin of fornix fibers which project to the mammillary bodies in the rat: a horseradish peroxidase study.		1975	49209
localization of laryngeal motor neurons in the kitten.	in a series of 12 newborn kittens, horseradish peroxidase (hrp) was used to trace retrograde axoplasmic flow in the motor neurons to laryngeal muscles. the animals were sacrificed 24 hours after injection of hrp into a specific laryngeal muscle, and the brain stems were stained for peroxidase. this clear-cut colorimetric method permitted the localization of the motor neurons in two nuclei of the ipsilateral brain stem. these are the nucleus ambiguus and the retrofacial nucleus. the primary sourc ...	1975	53770
electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage.	horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ...	1975	54168
retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat.	horseradish peroxidase (hrp) was injected into the pituitary in adult rats. after 2-3 days, the neurones of the supraoptic nuclei, the magnocellular parts of the paraventricular nuclei, and the various accessory neurosecretory hypothalamic nuclei showed accumulation of hrp. the hrp reaction product consisted of fine, discrete cytoplasmic granules, and in electron micrographys it was seen to be located in the lysosome-like dense bodies of 0.4-0.6 mum diameter which are normally present in the cyt ...	1975	49208
projections to the gyrus proreus from the brain stem tegmentum (locus coeruleus, raphe nuclei) in the cat, demonstrated by retrograde transport of horseradish peroxidase.		1975	50117
[uptake and transport of exogenous peroxidase in the masticatory muscles of cats. localization of ganglionic neurons].	retrograde axonal transport of horseradish peroxydase (hrp) has been used to trace the cells of origin of proprioceptive fibers in jaw-closing and jaw-opening muscles. after injection of hrp in young cats' masticatory muscles (masseteric, temporal, pterygoid, mylohyoid and digastric) labelled neurons were found in the ipsilateral semi-lunar ganglion and trigeminal mesencephalic nucleus. it is concluded that sensory endings are present in jaw-opening as in jaw-closing muscles; possibly the affere ...	1975	50149
[uptake and transport of exogenous peroxidase in the masticatory muscles of the cat. localization of motor neurons].	in the present work, a topographical localization of masticatory muscle motoneurons was undertaken. horseradish peroxydase injected in each muscle can be transported in the retrograde direction to the corresponding motoneurons cell bodies. jaw-closing muscle motoneurons were identified in the dorsal part of the motor trigeminal nucleus whereas jaw-opening muscle motoneurons were observed in the ventro-medial region.	1975	50152
studies on the cerebellar projections from the main and external cuneate nuclei in the cat by means of retrograde axonal transport of horseradish peroxidase.	the cerebellar projections from the main and external cuneate nuclei in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase. the main projection from the external cuneate nucleus (ecn) is to the intermediate and, possibly, the small lateral part of lobule v and to the paramedian lobule on the ipsilateral side. the projection from the ecn to the cerebellar regions mentioned is topographically organized. cells in the caudal part of the ecn send their axons t ...	1975	50867
ultrastructural localization of gamma-chain and immunoglobulin g in human lymphocytes using enzyme-labeled fab fragment.	by the use of rabbit antibodies against the heavy chain of human immunoglobulin g (igg), the gamma-chain and igg molecules were successfully localized at the ultrastructural level in human peripheral lymphocytes. the rabbit fab fragment was coupled to horseradish peroxidase by means of glutaraldehyde and the resulting conjugate could penetrate the intact plasma membrane. discernible reaction product was observed in cisternae of the nuclear envelope, rough endoplasmic reticulum and golgi apparatu ...	1975	51039
a study of the dynamics of retrograde transport and accumulation of horseradish peroxidase in injured neurons.	the phenomenon of retrograde intraaxonal transport of extracellular markers introduced at the level of the axon terminal has been suggested as a possible mechanism of communication between the axon terminal and the neuron cell body. we tested the hypothesis that communication after axotomy might consist of a change in the rate of uptake or of transport of material by injured neurons. small lesions were made with a needle in one retinal quadrant of chicks and immediately afterwards horseradish pe ...	1975	53088
preganglionic innervation of the adrenal gland of the rat: a study using horseradish peroxidase.		1975	53160
retrograde transport of horseradish peroxidase in transected axons. ii. relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis.	the sciatic nerves of 2--3 month-old mice, rats and rabbits were crushed and at the same time horseradish peroxidase (hrp) was applied around the crushed region at mid-thigh level. at varying time intervals thereafter, hrp was seen in neurons of the corresponding spinal ganglia and levels of the spinal cord. the rate of ascent of hrp appeared to be similar in the different animals and to be independent of the length of the nerve. the accumulation of hrp was more conspicuous in small than large n ...	1975	53277
rôle of axonal transport in maintaining central synaptic connections.	synaptic boutons on the somata of rat hypoglossal neurones have been stained with zinc iodide-osmium, and counted using light microscopy. two weeks after silastic cuffs containing colchicine or vinblastine were applied to the hypoglossal nerve, the number of boutons on parent nerve cell bodies was significantly lower than controls. these drugs inhibited retrograde axonal transport of horseradish peroxidase, but did not affect neuromuscular conduction or the number of axons in the nerve trunk. cu ...	1975	54262
retrograde transpost of horseradish peroxidase in afferent and efferent neurons from masseter muscle in the rat.		1975	54884
a comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method.	in this study we compared horseradish peroxidase (hrp)-labeled rabbit antihuman immunoglobulin g (igg) conjugates, prepared by a one-step and a two-step method. glutaraldehyde was used as a cross-linking agent. two methods were used for removing unconjugated hrp: sephadex g-200 gel chromatography and ammonium sulfate precipitation. the conjugates were characterized immunologically, immunochemically and enzymatically. the immunohistoenzymic properties of the conjugates were tested on unfixed cryo ...	1975	47869
olivocochlear and vestibular efferent neurons of the feline brain stem: their location, morphology and number determined by retrograde axonal transport and acetylcholinesterase histochemistry.	anterograde degeneration studies have shown that the cochlear and vestibular receptor organs receive an efferent innervation from neurons in the brain stem. this pathway may provide a mechanism by which the cns could modulate its own afferent input. the neurons which provide this innervation have so far escaped positive identification with methods which depend on retrograde cell changes after axotomy. in the present study, horseradish peroxidase (hrp) was injected into the labryinths of kittens ...	1975	47866
corticothalamic neurons and thalamocortical terminal fields: an investigation in rat using horseradish peroxidase and autoradiography.	subsequent to thalamic injections in rats of horseradish peroxidase (hrp) alone or hrp and [3h]leucine in combination, the cells of origin of the corticothalamic projections and the terminal fields of the thalamocortical projections were identified. hrp-labeled corticothalamic neurons were uniformly found in layers v and vi. they were medium to small in size and always pyramidal in shape with the larger neurons being found in layer v. on the other hand, 3 different patterns for the distribution ...	1975	46175
electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: possible involvement of the agranular reticulum.	the intracellular distribution of horeseradish peroxidase (hrp) transported intraaxonally from the caudoputaminal complex to the substantia nigra has been examined with the electron microscope. the reciprocal axonal connections between the caudoputamen and the substantia nigra permitted observation not only of hrp transported retrogradely from axons and axon terminals in the caudoputamen to the cell bodies of origin in the pars compacta of the substantia nigra, but also provided information sugg ...	1975	46174
a comparison of anterograde and retrograde axonal transport of horseradish peroxidase in the connections of the mammillary nuclei in the rat.		1975	46171
the orthograde transport of horseradish peroxidase in the visual system.		1975	46168
retrograde axonal transport of horseradish peroxidase in sensory nerves and ganglion cells of the rat.		1975	46159
thalamic afferents to areas 17, 18 and 19 of cat cortex traced with horseradish peroxidase.		1975	46157
retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick.		1975	46164
immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy.	a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ...	1975	56157
movement of horseradish peroxidase in the anterior parts of the eye [proceedings].		1975	61704
localization and quantitation of ebv-associated nuclear antigen (ebna) in raji cells.	epstein-barr virus (ebv)-associated nuclear antigen (ebna) was localized to chromatin fibers of raji cells. when ebna was labeled with the horseradish-peroxidase technique of sternberger a strong reaction covered most fiber surfaces as observed by electron microscopy. the dry mass of raji chromatin increased after exposure to both ebv-positive and -negative serum, but the increase was significantly larger in the material treated with positive serum.	1975	168861
cytochemical correlates of structural sexual dimorphism in glandular tissues of the mouse. i. studies of the renal glomerular capsule.	the parietal epithelium of bowman's capsule has been analyzed by enzyme cytochemistry in kidneys of mice (c57bl/6j) from birth to 50 days of age. there is a greater tendency for cells in the central portions of the capsular crescent to be cuboidal in post-pubertal males than in pre-pubertal mice of either sex or in post-pubertal females where they are generally squamous; moreover, these heightened capsular cells have a distinct microvillous border. cytochemical procedures were selected which mig ...	1975	171242
comparative ultrastructure and blood-brain barrier in diapause and nondiapause larvae of the european corn borer ostrinia nubilalis (hübner).	ultrastructural examination of diapause and nondiapause larval brains of the european corn borer disclosed anatomical differences that may be related to the insect's "blood-barrier". the perineurial type i cells are quite closely appressed in the diapause brain, but thrown into extensive folds with large intercellular spaces in the nondiapause brain. the perineurial type ii cells of diapause and nondiapause larvae are basically similar in general ultrastructure, and most likely form the basis fo ...	1975	126807
the interaction of 5-hydroxytryptamine and related hydroxyindoles with horseradish and mammalian peroxidase systems.		1975	234231
the permeability of muscle capillaries to horseradish peroxidase.	the main objective of this study was to determine the pathways by which horseradish peroxidase (hrp) can cross the endothelium of muscle capillaries. specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg hrp, at 4 min after intervenous injection of varied amounts of hrp, and at 4 min after intervenous injections in various volumes of isotonic nacl. our findings indicate that endothelial junctions serve as a barrier which ma ...	1975	169269
the mechanism of ferrocytochrome c oxidation by a horseradish isoperoxidase.	the oxidation of ferrocytochrome c catalysed by highly purified horse-radish isoperoxidase p2 was studied kinetically. to take into account the low turnover number of the enzyme and the tendency to autocatalytic oxidation of ferrocytochrome c, experimental conditions were used which prevented us from using the steady-state treatment. according to kinetic results reported by several authors, a kinetic scheme involving a ternary complex between the enzyme and the substrates was postulated and simu ...	1975	167872
electrical stimulation of the brain. iv. ultrastructural studies.	the ultrastructural response of tissues of the cat's central nervous system to chronic electrical stimulation with various parameters has been evaluated at four and a half days post stimulation. cellular alterations included widespread cytoplasmic vacuolations, architectural derangement and disruption of plasmalemma and other membranes. lipid inclusions and dense crystalline bodies were prominent in astrocyte-like phagocytic cells in the more severe lesions. despite endothelial damage in brain p ...	1975	171783
chicken neutrophils: oxidative metabolism in phagocytic cells devoid of myeloperoxidase.	polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated staphylococcus aureus. however, there was no demonstrable formate oxidation concomitant with phagocytosis in either the presence or absence of exogenous catalase. moreover, chicken polymorphonuclear leukocytes failed to oxidize scopoletin concomitant with phagocytosis in the presence ...	1975	174090
california encephalitis virus development in mosquitoes as revealed by transmission studies, immunoperoxidase staining, and electron microscopy.	isolates of the snowshoe hare subtype of california encephalitis (ce) virus from yukon mosquitoes during 1972 and 1973 were transmitted by bites of aedes aegypti mosquitoes after 4 to 5 weeks of extrinsic incubation at 55 degrees f after intrathoracic injection, and the 1973 strain was transmitted after mosquitoes were fed virus and held for 3 to 4 weeks at 75 degrees f. antigen of a 1971 isolate of ce virus (marsh lake 23) was detected in salivary glands of infected mosquitoes by the immunopero ...	1975	235355
endocytosis and multivesicular body formation in rabbit luteal cells during pseudopregnancy.	horseradish peroxidase (hrp) was injected directly into the corpora lutea of rabbits on different days of pseudopregnancy. young luteal cells have also been incubated "in vitro" in a medium containing horseradish peroxidase. in the "in vivo" experiments the 5 to 9 days old luteal cells take up far more horseradish peroxidase than the older ones (14-20 days). different types of endocytic vacuoles, mvb's and also some db's are already peroxidase positive shortly (15-20 min) after injection of the ...	1975	240507
immunochemical studies of the endocrine cells of the gastrointestinal tract. i. the use and value of peroxidase-conjugated antibody techniques for the localization of gastrin-containing cells in the human pyloric antrum.	this paper describes and immunochemical localization of gastrin-containing endocrine cells in the human pyloric antrum using antibodies conjugated to horseradish peroxidase. with pre-fixed cryostat sections, a distinct clear-cut staining of gastrin-containing cells can be obtained either by direct or indirect single stain procedures, but may cells containing endogenous peroxidase activity also stain. in order to abolish staining due to endogenous peroxidase, sections were pretreated with a numbe ...	1975	807542
certain connections of the visual cortex of the monkey shown by the use of horseradish peroxidase.		1975	809098
an electron microscopic study of retrograde and orthograde transport of horseradish peroxidase to the lateral geniculate nucleus of the monkey.		1975	809099
amygdaloid projections to prefrontal granular cortex in rhesus monkey demonstrated with horseradish peroxidase.		1975	810217
[demonstration of direct projections between the ponto-mesencephalic tegmentum and the visual cortex of the cat using a peroxidase technique].	using a retrograde tracer technique with horseradish peroxidase, we have identified the afferent connections to the visual cortex from the dorsolateral pontine tegmentum (locus coeruleus, nucleus parabrachialis medialis and nucleus parabrachialis lateralis) as well as from the dorsal raphe nucleus.	1975	813889
possible determinants of the degree of retrograde neuronal labeling with horseradish peroxidase.		1975	803395
is there a blood-brain barrier at the optic nerve head?	the retina and optic nerve have been demonstrated to possess a blood-brain barrier that prevents the passive passage of protein and certain dyes from the blood vessels into the extracellular space. our observations suggest that an exception is present at the normal optic disc. using horseradish peroxidase as a tracer for electron microscopy and the normal rhesus monkey as the experimental animal, we have demonstrated that in certain regions of the optic nerve head, horseradish peroxidase from th ...	1975	828849
the efferent connections of the suprachiasmatic nucleus of the hypothalamus.	the efferent connections of the suprachiasmatic nucleus of the hypothalamus have been studied in the rat by the injection of 3h-proline into the nucleus and the surrounding regions of the rostral hypothalamus, and by the injection of the enzyme marker, horseradish peroxidase, into the region of the ventromedial hypothalamic nucleus. after an injection of 3h-proline confined to the ventral portion of the suprachiasmatic nucleus, transported label can be followed, in the autoradiographs, dorsally ...	1975	803516
ocular vascular and epithelial barriers to microperoxidase.	microperoxidase (mp) is an ultrastructural tracer of small molecular weight (1,900) derived from horse heart cytochrome c. within the central nervous system, it is capable of entering the periaxonal space which is not open to horseradish peroxidase (hpr). because of its small size and unique behavior, mp was used to probe ocular vascular and epithelial barriers. mp did not enter any ocular space from which hrp was excluded. this tracer established a new lower level of permeability in these ocula ...	1975	806549
use of horseradish peroxidase-labelled antiglobulin for the colorimetric quantitation of erythrocyte antibodies.	horseradish peroxidase (hrp)-labelled antiglobulin has been used extensively as a histochemical marker. in the method described the stable reaction product formed by using 3,3'-diaminobenzidine (dab) as the hydrogen donor in the peroxidase reaction was dissolved in a fluoro alcohol, 1,1,1,3,3,3-hexafluoroisopropanol (hpf). reproducible standardisation curves resulted with hrp. based on these observations a colorimetric assay procedure was developed to quantitate the amount of antibody coating re ...	1975	811709
confirmation of the location of spinothalamic neurons in the cat and monkey by the retrograde transport of horseradish peroxidase.		1975	809119
histochemical characterization of a neocortical projection of the nucleus locus coeruleus in the squirrel monkey.	histochemical evidence is presented for a catecholamine-containing projection from the nucleus locus coeruleus to the neocortex in the squirrel monkey. the innervation of superior temporal gyrus has been examined in particular. glyoxylic acid-induced fluorescence shows an extensive arborization of fine, catecholamine-containing fibers with prominent varicosities in all layers of the neocortex. the nucleus locus coeruleus is identified as a source of these fibers by both ortho- and retrograde axo ...	1975	810499
monkey retinal ganglion cells: morphometric analysis and tracing of axonal projections, with a consideration of the peroxidase technique.	this paper presents evidence on the retinal distribution and central projections of retinal ganglion cells of various cell body sizes in the adult macaque monkey. the ganglion cell sizes have been determined by computer assisted measurement of camera lucida drawings at various eccentricities of both flat mounted and sectioned retinae. the pattern of projections of individual ganglion cells to the dorsal lateral geniculate nucleus and superior colliculus has been studied using retrograde axonal t ...	1975	810500
the origin of efferent pathways from the primary visual cortex, area 17, of the macaque monkey as shown by retrograde transport of horseradish peroxidase.	the retrograde transport of horseradish peroxidase has been used to identify efferent cells in area 17 of the macaque. cells projecting to the lateral geniculate nucleus are small to medium sized pyramidal neurons with somata in lamina 6 and the adjacent white matter. the projection to the parvocellular division arises preferentially from the upper half of lamina 6, while that to the magnocellular division arises preferentially from the lower part of the lamina. the projection to both superior c ...	1975	810501
attempts at differentiation of the structure of neurons in the corpus geniculatum laterale of the pig by the immunocytochemical technique.	the investigations were carried out on the corpus geniculatum laterale of the pig with the aim to differentiate the structure of its neurons on the basis of the antigenic properties of the nervous tissue. the antigenic material consisted of the homogenate of the corpus geniculatum laterale (h) and of its nuclear (p1) and mitochondrial (p2) fractions. the antigens were used to immunize 3 groups of rabbits, the 4th one serving as control. the antigens were prepared according to requirements of the ...	1975	812788
trigeminal representations of the masticatory and extraocular proprioceptors as revealed by horseradish peroxidase retrograde transport.	horseradish peroxidase has been injected in the masticatory and extraocular muscles in newborn and adult cats to identify the cells of origin for the muscle endings. labeled motoneurons in the nuclei of the iii, iv, v and vi nerves have been observed. they are the parent cells of the motor terminals taking up the enzyme in the muscle injected. labeled ganglionic cells have been found scattered all along the ipsilateral mesencephalic nucleus of the v nerve after injection of both the jaw closing ...	1975	1081052
demonstration of immunoglobulin containing deposits in glomerular basement membrane in experimental chronic serum sickness using horseradish peroxidase labelled antiserum.	horseradish peroxidase (hrp) labelled antibodies were used in rabbit glomerulonephritis to obtain localization of immunoglobulins in the glomerulus at a light and electron microscope level. better localization of deposits was obtained with peroxidase labelled antiserum than with immunofluorescence at the light microscope level. ultrastructurally, immunoglobulin localization was seen to correspond to the electron dense deposits demonstrated in and on the glomerular basement membrane by convention ...	1975	1106748
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of surface antigens in pure cultures.	an indirect method of localizing antigens with horseradish peroxidase-labeled antibody was used to identify and localize surface antigens of streptococcus sanguis at the ultrastructural level. an electron dense layer surrounding the cell wall could be distinguished without any additional electron microscope staining. this labeled layer represents an immune complex consisting of bacterial surface antigens, specific rabbit antisera, and peroxidase-labeled goat anti-rabbit globulins. although with ...	1975	1090524
the pulmonary inflammatory response. cellular events in experimental pulmonary arterial hypersensitivity disease.	horseradish peroxidase (hrp) or bovine serum albumin (bsa) were covalently linked to polyacrylamide or agarose beads and were injected into control syrian hamsters and hamsters previously immunized with either hrp or bsa. animals sensitized to soluble antigen and subsequently challenged intravenously with the same antigen immobilized on beads developed an acute focal inflammatory response within 2 to 6 hours after injection. the acute response involved local deposition of igg and complement (bet ...	1975	1092187
subcortical afferents to the frontal lobe in the rhesus monkey studied by means of retrograde horseradish peroxidase transport.		1975	1111842
a combined horseradish peroxidase-autoradiographic investigation of reciprocal connections between superior temporal gyrus and pulvinar in squirrel monkey.		1975	1111845
basal forebrain and hypothalamic connection to frontal and parietal cortex in the rhesus monkey.	horseradish peroxidase was injected in different parts of the frontal and parietal cortex in 17 rhesus monkeys. in all cases the enzyme was transported retrogradely to neurons in the substantia innominata and hypothalamus as well as in the thalamus. these new findings demonstrate that these cortical areas receive direct afferent fibers from limbic basal forebrain areas concerned with emotion and motivation.	1975	1114317
acquisition of host antigens by young schistosoma mansoni in mice: correlation with failure to bind antibody in vitro.	young schistosomes collected after penetration through isolated mouse skin (3 h schistosomula) were cultured in medium containing immune rhesus monkey serum with a high titre of antibody known to kill schistosomula in the presence of complement. morphological signs of damage in electron micrographs were confined to the surface tegument of the schistosomula. antibodies in immune rhesus serum were shown to bind to the surface membrane of 3 h schistosomula using an antibody-enzyme bridge technique ...	1975	1118189
application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system.	immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. the immunoperoxidase bridge techniques from reaction products of high quality which c ...	1975	1127219
macromolecular transport by the middle ear and its lymphatic system.	the macromolecular transport in the middle ear mucosa of the guinea pig was investigated by means of light and electron microscopy using tracer substances such as evans blue, india ink, and horseradish peroxidase (hrp). hrp particles were readily taken up by the middle ear mucosa and eustachian tube. they were absorbed by all cell types, including ciliated, secretory and nonsecretory cells. the particles were first taken up by the pinocytotic vesicles and then transported into the intercellular ...	1975	1166775
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of streptococcus sanguis in intact dental plaque.	streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. the technique allows for complete diffusion of the reagents to all portions of the plaque specimens. control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. the 30-mum-thick sections can be examined in the light microscope to localize ar ...	1975	1167846
mesencephalic porjections of the rectus lateralis muscle afferents in the cat.	responses to passive stretch applied to the rectus lateralis muscle (rl) were recorded with a microelectrode from the mesencephalic nucleus of the fifth nerve (mes v) in cat encéphale isolé preparation. the necessary conditions to attribute the unit response to an eye muscle stretch are the followings: i) respond neither to jaw movements nor to pressure applied to the eye ball: ii) be activated with a short latency, following stretch of the eye muscles; iii) be excited by a light touch applied t ...	1975	1080407
permeability of rabbit corneal epithelium to horseradish peroxidase after the influence of benzalkonium chloride.	a tight barrier against permeation of horseradish peroxidase into the corneal epithelium exists at the corneal surface adjacent to the tear film. the present light and transmission electron microscopic study reveals that the cationic surfactant, benzalkonium chloride, which is commonly added to eye drops as a preservative, breaks down this barrier. lysis of the cell membranes was demonstrated, resulting in a leakage of the tracer into and underneath the superficial cells. the grade of cellular d ...	1975	1174396
morphology of the breakdown of the blood-aqueous barrier in the ciliary processes of the rabbit eye after prostaglandin e2.	protaglandin e2, administered topically to the rabbit eye, causes disruption of the blood-aqueous barrier resulting in a large increase in the protein content of the aqueous humor. the route of plasma proteins into the aqueous humor was studied with the electron microscope, using horseradish peroxidase as a protein tracer. the tracer penetrated the tight junctions of the nonpigmented layer of the ciliary epithelium, filling the lateral intercellular clefts and staining the internal limiting mem ...	1975	1110133
chorioretinal diffusion processes following pigment epithelial degeneration.	we studied the effect of sodium iodate-induced pigment epithelial degeneration on chorioretinal diffusion processes. rabbits received two intravenous injections of sodium iodate at the retinotoxic dose of 22.5 mg. per kilogram over a six- to eight-hour period. combined horseradish peroxidase tracing technique and electron microscopy were used to compare diffusion processes over a five-week period. ultrastructural examination of retina twenty-four hours after iodate administration revealed pigmen ...	1975	1110139
permeability properties of the postcapillary high-endothelial venules in lymph nodes of the mouse.	the permeability of the postcapillary high-endothelial venules in mice was investigated by light and electron microscopy. experiments with intravenously injected horseradish peroxidase demonstrated a pronounced intercellular passage of this tracer. vesicular transport was not observed. additional experiments with lanthanum and tannic acid showed that no continous tight junctions sealed the intercellular spaces. however, some focal membrane appositions were observed.	1975	1113511
permeability of ovarian follicles and capillaries in mice.	the permeability of ovarian capillaries and follicles in prepubertal and sexually mature (proestrus and metestrus) randomly bred swiss albino female mice (sch:ars ha icr strain) was studied by intravenous injection of either ferritin or horseradish peroxidase (hrp), followed by examination with light and electron microscopes. the study revealed that capillaries in the interstitial and perifollicular regions were provided with a continuous endothelium that had constant permeability characteristic ...	1975	1119413
ultrastructural studies on cerebrovascular permeability in acute hypertension.	acute hypertension, experimentally induced by intravenous injection of metaraminol in adult rabbits, rapidly induced a damage of the blood-brain barrier in the cerebral cortex, as visualized by evans-blue-conjugated albumin and horseradish peroxidase. extravasation of these two exogenous tracers was demonstrated to occur in arterioles, in capillaries and, rarely, in venules. peroxidase passed the endothelial cell into the nervous tissue in either or three different ways, i.e. through channels, o ...	1975	1180001
mössbauer investigations of high-spin ferrous heme proteins. i. cytochrome p-450.	anaerobically reduced samples of cytochrome p-450 from pseudomonas putida were studied by mössbauer spectroscopy. in the presence of an applied magnetic field the high-spin ferrous heme iron showed an intricate pattern of electric and magnetic hyperfine interactions which could be parametrized successfully in terms of a spin hamiltonian formalism. the results imply a very low (triclinic) symmetry of the heme iron. the effects of the ligand environment and of spin-orbit coupling result in a large ...	1975	1182094