Publications

an efferent connection of the solitary cells of meynert. a study with horseradish peroxidase in the marmoset callithrix.		1975	809097
localization of alpha-d-glucosyl and alpha-d-mannosyl groups of mucosubstances with concanavalin a and horseradish peroxidase.	concanavalin a is a lectin which is known to bind specifically to alpha-d-glucosyl and alpha-d-mannosyl groups in the mucosubstances of mammalian tissues. the lectin molecule is bivalent; after its attachment to mucosubstances present in a histological specimen it can also bind horseradish peroxidase, a mannose-containing glycoprotein. the attached peroxidase may then be visualized by virtue of its histochemically demonstrable enzymatic activity. other investigators have utilized this principle ...	1975	52638
photochemical reactions of horseradish peroxidase compounds i and ii at room temperature and 13 degrees k.	some photochemical reactions of horseradish peroxidase compounds i and ii (hrp-i and hrp-ii, respectively) have been studied by electronic absorption spectroscopy over the temperature range 297 degrees k-10 degrees k. in glassy matrices below 80 degrees k hrp-i is rapidly converted to hrp-ii when irradiated with low power white light. the native enzyme and hrp-ii are not photochemically active at these temperatures with low power irradiation. at room temperature the spontaneous decay of both hrp ...	1975	1148197
effects of 2,4-substituents of deuteropheme upon redox potentials of horseradish peroxidases.		1975	239639
epr studies on compound i of horseradish peroxidase.	compound i of horseradish peroxidase (donor: hydrogen-peroxide oxidoreductase ec 1.11.1.7) was studied by epr at low temperatures. an asymmetric signal was found, about 15 gauss wide and with a g-value of 1.995, which could be detected only at temperatures below 20 k and which had an intensity corresponding to about 1% of the heme content. in a titration with h2o2, the signal intensity was proportional to the concentration of compound i, reaching a maximum when equivalent amounts of h2o2 were ad ...	1975	167831
distribution of ultrastructural tracers in crustacean axons.	ruthenium red and horseradish peroxidase were used to compare the uptake of exogenous molecules into crayfish motor axons and their sheaths in severed and intact peripheral nerves. both tracers penetrated the axonal sheath and were subsequently seen lining small vesicles and tubules in the axoplasm. tracer appeared to enter the axon via pinocytotic vesicles. there were no perceptible quantitative or qualitative differences in ruthenium red uptake between intact and severed axons. however, counts ...	1975	1133229
the ultrastructure of normal and glycerol treated muscle in the ghost crab, ocypode cursor.	the ultrastructure of normal and glycerol treated fibers of the closer muscle of the ghost crab, ocypode cursor, was studiedmthe muscle is composed of presumably phasic (short sarcomeres) and tonic (long sarcomeres) fibers, the latter greatly predominating. horseradish peroxidase (hrp) was used as an extracellular tracer to delineate the tubular system (ts), and to determine to what extent this system becomes detached from the extracellular space as a result of glycerol treatment. sarcolemmal cl ...	1975	1149103
titration study of guaiacol oxidation by horseradish peroxidase.	titration of guaiacol by hydrogen peroxide in the presence of a catalytic amount of horseradish peroxidase shows that the reduction of hydrogen peroxide proceeds by the abstraction of two electrons from a guaiacol molecule. in the same way, it can be demonstrated that 0.5 mol of guaiacol can reduce, at low temperature, 1 mol of peroxidase compound i to compound ii. moreover, the reaction between equal amounts of compound i and guaiacol at low temperature produces the native enzyme. a reaction sc ...	1975	1139404
the results of freezing and dehydration of horseradish peroxidase.		1975	1132241
the horseradish peroxidase technique applied to the teleostean nervous system.		1975	50115
absorption and transport of ferritin and exogenous horseradish peroxidase in the opisthonephric kidney of the sea lamprey. i. the renal corpuscle.		1975	1131752
the effects of prolonged repetitive stimulation in hemicholinium on the frog neuromuscular junction.	1. cutaneous pectoris nerve-muscle preparations from the frog were stimulated for prolonged periods in solutions with curare alone, curare and hemicholinium no. 3 (hc-3), or curare and glucose plus choline. end-plate potentials (e.p.p.s) and miniature end-plate potentials (m.e.p.p.s) were recorded intracellularly. black widow spider venom (bwsv) was applied to determine the degree of depletion of the transmitter stores. 2. the ultrastructure of the neuromuscular junctions was studied in the elec ...	1975	1079538
a kinetic study of the reaction of horseradish peroxidase with hydrogen peroxide.	a kinetic study has been carried out over the ph range of 2.63-9.37 for the reaction of horseradish peroxidase with hydrogen peroxide to form compound i of th;e enzyme. analysis of the results, indicates that there are two kinetic influencing, ionizable groups on the enzyme with pka values of 3.2 and 3.9. protonation of these groups results in a decrease in the rate of reaction of the enzyme with h2o2. a previous study of the kinetics of cyanide binding to horseradish peroxidase (ellis, w.d. & d ...	1975	237615
nuclear magnetic resonance studies of hemoproteins. binding of aromatic donor molecules to horseradish peroxidase.		1975	429320
retrograde axonal transport of horseradish peroxidase from spinal cord to brain stem cell groups in the cat.		1975	19604744
stimulation-induced uptake of horseradish peroxidase by rat cortical synapses.		1975	19604745
labelling of cells in the medulla oblongata and the spinal cord of the monkey after injections of horseradish peroxidase in the thalamus.	injections of horseradish peroxidase were performed in macaque monkeys in the intralaminar and the ventroposterior thalamic region, respectively. following injection in the intralaminar region labelled cells were present in the marginal zone both in the spinal trigeminal nuclues and in the spinal dorsal horn. the same areas contained labelled cells after injections in the ventroposterior thalamic region, but in such experiments labelled cells were also present in the lamina iv-v region of the sp ...	1975	19604756
the reaction of coumarins with horseradish peroxidase.	the peroxidase catalyzed oxidation of indole-3-acetate is inhibited by naturally occurring coumarins such as scopoletin. this inhibition is due to the preferential reactivity of the coumarins with the peroxidase compounds i, ii, and iii. in view of the possible growth regulatory role of coumarins in plants, the mechanism of oxidation of scopoletin by horse-radish peroxidase has been investigated.peroxidase catalyzed coumarin oxidation requires either an electron donor and molecular oxygen or hyd ...	1975	16659024
the isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish.	the relationship of indoleacetic acid oxidase activity to peroxidase activity is complicated by numerous multiple forms of this enzyme system. it is not known if all isozymes of this complex system contain both types of activity. isozyme analysis of commercial horseradish peroxidase and leaf extracts of yellow birch (betula alleghaniensis) by isoelectric focusing in polyacrylamide gels was used to examine this problem. horseradish and birch exhibited 20 and 13 peroxidase isozymes, respectively, ...	1975	16659371
in vitro studies on the selective binding of igg from different species to tissue sections of the bovine mammary gland.	tissue sections of the mammary gland of cattle provide a sensitive and reproducible model for studying the initial events of the selective transport of bovine iggs by the acinar epithelium (ae). mammary tissue was reacted with purified antibody to horseradish peroxidase (hrp) and exposed to hrp. the sites of peroxidase activity were revealed cytochemically. the highly selective binding of iggs by ae of the colostrum-forming gland but never of the lactating gland was most notable, whereas igg1 an ...	1975	11993319
junctional complexes in the preimplantation rabbit embryo.	the morphology and development of junctional complexes between blastomeres of the preimplantation rabbit embryo were investigated using several approaches. electron microscopic examination of embryos stained en bloc with uranyl acetate, and the study of junction permeability using horseradish peroxidase and lanthanum nitrate provided information on structure, intermembrane spacing and permeability of the junctional complexes. in addition, the freeze fracture technique was used with day 5 and day ...	1975	45878
localization of immunoreactive androgen in testicular tissue.	immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. the bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. the highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferou ...	1975	45910
thalamic afferents to areas 17, 18 and 19 of cat cortex traced with horseradish peroxidase.		1975	46157
retrograde axonal transport of horseradish peroxidase in sensory nerves and ganglion cells of the rat.		1975	46159
retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick.		1975	46164
the orthograde transport of horseradish peroxidase in the visual system.		1975	46168
a comparison of anterograde and retrograde axonal transport of horseradish peroxidase in the connections of the mammillary nuclei in the rat.		1975	46171
electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: possible involvement of the agranular reticulum.	the intracellular distribution of horeseradish peroxidase (hrp) transported intraaxonally from the caudoputaminal complex to the substantia nigra has been examined with the electron microscope. the reciprocal axonal connections between the caudoputamen and the substantia nigra permitted observation not only of hrp transported retrogradely from axons and axon terminals in the caudoputamen to the cell bodies of origin in the pars compacta of the substantia nigra, but also provided information sugg ...	1975	46174
corticothalamic neurons and thalamocortical terminal fields: an investigation in rat using horseradish peroxidase and autoradiography.	subsequent to thalamic injections in rats of horseradish peroxidase (hrp) alone or hrp and [3h]leucine in combination, the cells of origin of the corticothalamic projections and the terminal fields of the thalamocortical projections were identified. hrp-labeled corticothalamic neurons were uniformly found in layers v and vi. they were medium to small in size and always pyramidal in shape with the larger neurons being found in layer v. on the other hand, 3 different patterns for the distribution ...	1975	46175
use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy.	staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. the topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. the technique is therefore a useful aid to the study of cultured cells with phase opti ...	1975	46631
genetic control of sensitization to structurally unrelated antigens and its relationship to histocompatibility antigens in guinea-pigs.	five strains of guinea-pigs homozygous for gpl-a and 2/13 histocompatibility antigens were sequentially immunized with seven structurally unrelated antigens (tgal, bpo-bgg, asan, bpo-pll, phenetidine, horseradish peroxidase and dnp-dodecapeptide). considerable differences in the immune response were observed, as measured by delayed skin reactivity and antigen-induced lymphocyte stimulation in vitro. the data presented show that when the progeny of various back-cross matings are analysed for thei ...	1975	47308
olivocochlear and vestibular efferent neurons of the feline brain stem: their location, morphology and number determined by retrograde axonal transport and acetylcholinesterase histochemistry.	anterograde degeneration studies have shown that the cochlear and vestibular receptor organs receive an efferent innervation from neurons in the brain stem. this pathway may provide a mechanism by which the cns could modulate its own afferent input. the neurons which provide this innervation have so far escaped positive identification with methods which depend on retrograde cell changes after axotomy. in the present study, horseradish peroxidase (hrp) was injected into the labryinths of kittens ...	1975	47866
a comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method.	in this study we compared horseradish peroxidase (hrp)-labeled rabbit antihuman immunoglobulin g (igg) conjugates, prepared by a one-step and a two-step method. glutaraldehyde was used as a cross-linking agent. two methods were used for removing unconjugated hrp: sephadex g-200 gel chromatography and ammonium sulfate precipitation. the conjugates were characterized immunologically, immunochemically and enzymatically. the immunohistoenzymic properties of the conjugates were tested on unfixed cryo ...	1975	47869
the retrograde transport method.	after an injection of horseradish peroxidase into the brain, the enzyme product can later be found in cell bodies of neurons which project axons to the injection site. the use of the tracer has now been adapted as a means of identifying neurons on the basis of their efferent fiber projections. in this review particular attention is paid to characteristics of the phenomenon of retrograde transport, such as diffusion of the marker from the injection site and the region of effective uptake, uptake ...	1975	48482
retrograde axonal transport of horseradish peroxidase in peripheral autonomic nerves.	an exogeneous marker protein, horseradish peroxidase (hrp) was used to race peripheral autonomic pathways in adult guinea pigs and cats. small doses of hrp were injected into various organs and after a brief survival period, hrp activity appeared in the perikarya of autonomic neurons that supplied each injection site. after injection of hrp into the anterior chamber of the eye, reaction product was detected in the postganglionic sympathetic neurons of the superior cervical sympathetic ganglion. ...	1975	48519
retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat.	horseradish peroxidase (hrp) was injected into the pituitary in adult rats. after 2-3 days, the neurones of the supraoptic nuclei, the magnocellular parts of the paraventricular nuclei, and the various accessory neurosecretory hypothalamic nuclei showed accumulation of hrp. the hrp reaction product consisted of fine, discrete cytoplasmic granules, and in electron micrographys it was seen to be located in the lysosome-like dense bodies of 0.4-0.6 mum diameter which are normally present in the cyt ...	1975	49208
the origin of fornix fibers which project to the mammillary bodies in the rat: a horseradish peroxidase study.		1975	49209
demonstration of nigrothalamic connections in the cat by retrograde axonal transport of horseradish peroxidase.		1975	49210
purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization.	mouse alpha1-fetoprotein (afp) was isolated from amniotic fluid by immunoadsorbent columns and preparative electrophoresis. specific antibodies were isolated from monospecific hyperimmunsera by use of immunoadsorbents, and subsequently coupled with horseradish peroxidase. at the light microscopical level, purified antibody-peroxidase conjugates were used for the cellular localization of afp in fetal liver by direct and indirect staining methods. fixatives containing ethanol or aldehydes were tri ...	1975	49343
caveola--vesicle complexes in the plasmalemma of erythrocytes infected by plasmodium vivax and p cynomolgi. unique structures related to schüffner's dots.	erythrocytes infected with plasmodium vivax and p cynomolgi, characterized by schüffner's dots on giemsa-stained thin films, were studied by electron microscopy and immunocytochemistry. a caveola-vesicle complex, which consisted of a caveola surrounded by vesicles, in an alveolar fashion, formed along the erythrocyte plasmalemma. horseradish-peroxidase-labeled immunoglobulin from a monkey infected with p vivax bound to the vesicle membrane. cationized ferritin appeared within the vesicles after ...	1975	50017
projections to the gyrus proreus from the brain stem tegmentum (locus coeruleus, raphe nuclei) in the cat, demonstrated by retrograde transport of horseradish peroxidase.		1975	50117
[uptake and transport of exogenous peroxidase in the masticatory muscles of cats. localization of ganglionic neurons].	retrograde axonal transport of horseradish peroxydase (hrp) has been used to trace the cells of origin of proprioceptive fibers in jaw-closing and jaw-opening muscles. after injection of hrp in young cats' masticatory muscles (masseteric, temporal, pterygoid, mylohyoid and digastric) labelled neurons were found in the ipsilateral semi-lunar ganglion and trigeminal mesencephalic nucleus. it is concluded that sensory endings are present in jaw-opening as in jaw-closing muscles; possibly the affere ...	1975	50149
[uptake and transport of exogenous peroxidase in the masticatory muscles of the cat. localization of motor neurons].	in the present work, a topographical localization of masticatory muscle motoneurons was undertaken. horseradish peroxydase injected in each muscle can be transported in the retrograde direction to the corresponding motoneurons cell bodies. jaw-closing muscle motoneurons were identified in the dorsal part of the motor trigeminal nucleus whereas jaw-opening muscle motoneurons were observed in the ventro-medial region.	1975	50152
studies on the cerebellar projections from the main and external cuneate nuclei in the cat by means of retrograde axonal transport of horseradish peroxidase.	the cerebellar projections from the main and external cuneate nuclei in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase. the main projection from the external cuneate nucleus (ecn) is to the intermediate and, possibly, the small lateral part of lobule v and to the paramedian lobule on the ipsilateral side. the projection from the ecn to the cerebellar regions mentioned is topographically organized. cells in the caudal part of the ecn send their axons t ...	1975	50867
ultrastructural localization of gamma-chain and immunoglobulin g in human lymphocytes using enzyme-labeled fab fragment.	by the use of rabbit antibodies against the heavy chain of human immunoglobulin g (igg), the gamma-chain and igg molecules were successfully localized at the ultrastructural level in human peripheral lymphocytes. the rabbit fab fragment was coupled to horseradish peroxidase by means of glutaraldehyde and the resulting conjugate could penetrate the intact plasma membrane. discernible reaction product was observed in cisternae of the nuclear envelope, rough endoplasmic reticulum and golgi apparatu ...	1975	51039
the identification of striatal and pallidal neurons projecting to substantia nigra. an experimental study by means of retrograde axonal transport of horseradish peroxidase.		1975	51667
a study of the dynamics of retrograde transport and accumulation of horseradish peroxidase in injured neurons.	the phenomenon of retrograde intraaxonal transport of extracellular markers introduced at the level of the axon terminal has been suggested as a possible mechanism of communication between the axon terminal and the neuron cell body. we tested the hypothesis that communication after axotomy might consist of a change in the rate of uptake or of transport of material by injured neurons. small lesions were made with a needle in one retinal quadrant of chicks and immediately afterwards horseradish pe ...	1975	53088
preganglionic innervation of the adrenal gland of the rat: a study using horseradish peroxidase.		1975	53160
retrograde transport of horseradish peroxidase in transected axons. ii. relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis.	the sciatic nerves of 2--3 month-old mice, rats and rabbits were crushed and at the same time horseradish peroxidase (hrp) was applied around the crushed region at mid-thigh level. at varying time intervals thereafter, hrp was seen in neurons of the corresponding spinal ganglia and levels of the spinal cord. the rate of ascent of hrp appeared to be similar in the different animals and to be independent of the length of the nerve. the accumulation of hrp was more conspicuous in small than large n ...	1975	53277
localization of laryngeal motor neurons in the kitten.	in a series of 12 newborn kittens, horseradish peroxidase (hrp) was used to trace retrograde axoplasmic flow in the motor neurons to laryngeal muscles. the animals were sacrificed 24 hours after injection of hrp into a specific laryngeal muscle, and the brain stems were stained for peroxidase. this clear-cut colorimetric method permitted the localization of the motor neurons in two nuclei of the ipsilateral brain stem. these are the nucleus ambiguus and the retrofacial nucleus. the primary sourc ...	1975	53770
electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage.	horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ...	1975	54168
rôle of axonal transport in maintaining central synaptic connections.	synaptic boutons on the somata of rat hypoglossal neurones have been stained with zinc iodide-osmium, and counted using light microscopy. two weeks after silastic cuffs containing colchicine or vinblastine were applied to the hypoglossal nerve, the number of boutons on parent nerve cell bodies was significantly lower than controls. these drugs inhibited retrograde axonal transport of horseradish peroxidase, but did not affect neuromuscular conduction or the number of axons in the nerve trunk. cu ...	1975	54262
hepatic nucleases. extrahepatic origin and association of neutral liver ribonuclease with lysosomes.	in the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by triton wr-1339 accumulation in lysosomes. particulate neutral ribonuclease is latent; the enzyme is unmasked by very low digitonin concentrations or hypoosmotic shock. these observations demonstrate that the bulk of liver neutral ribonuclease is associated with the lysosomal system. in view of ...	1975	1273
immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy.	a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ...	1975	56157
movement of horseradish peroxidase in the anterior parts of the eye [proceedings].		1975	61704
retrograde transpost of horseradish peroxidase in afferent and efferent neurons from masseter muscle in the rat.		1975	54884
the mechanism of ferrocytochrome c oxidation by a horseradish isoperoxidase.	the oxidation of ferrocytochrome c catalysed by highly purified horse-radish isoperoxidase p2 was studied kinetically. to take into account the low turnover number of the enzyme and the tendency to autocatalytic oxidation of ferrocytochrome c, experimental conditions were used which prevented us from using the steady-state treatment. according to kinetic results reported by several authors, a kinetic scheme involving a ternary complex between the enzyme and the substrates was postulated and simu ...	1975	167872
localization and quantitation of ebv-associated nuclear antigen (ebna) in raji cells.	epstein-barr virus (ebv)-associated nuclear antigen (ebna) was localized to chromatin fibers of raji cells. when ebna was labeled with the horseradish-peroxidase technique of sternberger a strong reaction covered most fiber surfaces as observed by electron microscopy. the dry mass of raji chromatin increased after exposure to both ebv-positive and -negative serum, but the increase was significantly larger in the material treated with positive serum.	1975	168861
the permeability of muscle capillaries to horseradish peroxidase.	the main objective of this study was to determine the pathways by which horseradish peroxidase (hrp) can cross the endothelium of muscle capillaries. specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg hrp, at 4 min after intervenous injection of varied amounts of hrp, and at 4 min after intervenous injections in various volumes of isotonic nacl. our findings indicate that endothelial junctions serve as a barrier which ma ...	1975	169269
cytochemical correlates of structural sexual dimorphism in glandular tissues of the mouse. i. studies of the renal glomerular capsule.	the parietal epithelium of bowman's capsule has been analyzed by enzyme cytochemistry in kidneys of mice (c57bl/6j) from birth to 50 days of age. there is a greater tendency for cells in the central portions of the capsular crescent to be cuboidal in post-pubertal males than in pre-pubertal mice of either sex or in post-pubertal females where they are generally squamous; moreover, these heightened capsular cells have a distinct microvillous border. cytochemical procedures were selected which mig ...	1975	171242
electrical stimulation of the brain. iv. ultrastructural studies.	the ultrastructural response of tissues of the cat's central nervous system to chronic electrical stimulation with various parameters has been evaluated at four and a half days post stimulation. cellular alterations included widespread cytoplasmic vacuolations, architectural derangement and disruption of plasmalemma and other membranes. lipid inclusions and dense crystalline bodies were prominent in astrocyte-like phagocytic cells in the more severe lesions. despite endothelial damage in brain p ...	1975	171783
chicken neutrophils: oxidative metabolism in phagocytic cells devoid of myeloperoxidase.	polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated staphylococcus aureus. however, there was no demonstrable formate oxidation concomitant with phagocytosis in either the presence or absence of exogenous catalase. moreover, chicken polymorphonuclear leukocytes failed to oxidize scopoletin concomitant with phagocytosis in the presence ...	1975	174090
comparative ultrastructure and blood-brain barrier in diapause and nondiapause larvae of the european corn borer ostrinia nubilalis (hübner).	ultrastructural examination of diapause and nondiapause larval brains of the european corn borer disclosed anatomical differences that may be related to the insect's "blood-barrier". the perineurial type i cells are quite closely appressed in the diapause brain, but thrown into extensive folds with large intercellular spaces in the nondiapause brain. the perineurial type ii cells of diapause and nondiapause larvae are basically similar in general ultrastructure, and most likely form the basis fo ...	1975	126807
the interaction of 5-hydroxytryptamine and related hydroxyindoles with horseradish and mammalian peroxidase systems.		1975	234231
california encephalitis virus development in mosquitoes as revealed by transmission studies, immunoperoxidase staining, and electron microscopy.	isolates of the snowshoe hare subtype of california encephalitis (ce) virus from yukon mosquitoes during 1972 and 1973 were transmitted by bites of aedes aegypti mosquitoes after 4 to 5 weeks of extrinsic incubation at 55 degrees f after intrathoracic injection, and the 1973 strain was transmitted after mosquitoes were fed virus and held for 3 to 4 weeks at 75 degrees f. antigen of a 1971 isolate of ce virus (marsh lake 23) was detected in salivary glands of infected mosquitoes by the immunopero ...	1975	235355
endocytosis and multivesicular body formation in rabbit luteal cells during pseudopregnancy.	horseradish peroxidase (hrp) was injected directly into the corpora lutea of rabbits on different days of pseudopregnancy. young luteal cells have also been incubated "in vitro" in a medium containing horseradish peroxidase. in the "in vivo" experiments the 5 to 9 days old luteal cells take up far more horseradish peroxidase than the older ones (14-20 days). different types of endocytic vacuoles, mvb's and also some db's are already peroxidase positive shortly (15-20 min) after injection of the ...	1975	240507
morphology of the breakdown of the blood-aqueous barrier in the ciliary processes of the rabbit eye after prostaglandin e2.	protaglandin e2, administered topically to the rabbit eye, causes disruption of the blood-aqueous barrier resulting in a large increase in the protein content of the aqueous humor. the route of plasma proteins into the aqueous humor was studied with the electron microscope, using horseradish peroxidase as a protein tracer. the tracer penetrated the tight junctions of the nonpigmented layer of the ciliary epithelium, filling the lateral intercellular clefts and staining the internal limiting mem ...	1975	1110133
chorioretinal diffusion processes following pigment epithelial degeneration.	we studied the effect of sodium iodate-induced pigment epithelial degeneration on chorioretinal diffusion processes. rabbits received two intravenous injections of sodium iodate at the retinotoxic dose of 22.5 mg. per kilogram over a six- to eight-hour period. combined horseradish peroxidase tracing technique and electron microscopy were used to compare diffusion processes over a five-week period. ultrastructural examination of retina twenty-four hours after iodate administration revealed pigmen ...	1975	1110139
subcortical afferents to the frontal lobe in the rhesus monkey studied by means of retrograde horseradish peroxidase transport.		1975	1111842
a combined horseradish peroxidase-autoradiographic investigation of reciprocal connections between superior temporal gyrus and pulvinar in squirrel monkey.		1975	1111845
permeability properties of the postcapillary high-endothelial venules in lymph nodes of the mouse.	the permeability of the postcapillary high-endothelial venules in mice was investigated by light and electron microscopy. experiments with intravenously injected horseradish peroxidase demonstrated a pronounced intercellular passage of this tracer. vesicular transport was not observed. additional experiments with lanthanum and tannic acid showed that no continous tight junctions sealed the intercellular spaces. however, some focal membrane appositions were observed.	1975	1113511
basal forebrain and hypothalamic connection to frontal and parietal cortex in the rhesus monkey.	horseradish peroxidase was injected in different parts of the frontal and parietal cortex in 17 rhesus monkeys. in all cases the enzyme was transported retrogradely to neurons in the substantia innominata and hypothalamus as well as in the thalamus. these new findings demonstrate that these cortical areas receive direct afferent fibers from limbic basal forebrain areas concerned with emotion and motivation.	1975	1114317
acquisition of host antigens by young schistosoma mansoni in mice: correlation with failure to bind antibody in vitro.	young schistosomes collected after penetration through isolated mouse skin (3 h schistosomula) were cultured in medium containing immune rhesus monkey serum with a high titre of antibody known to kill schistosomula in the presence of complement. morphological signs of damage in electron micrographs were confined to the surface tegument of the schistosomula. antibodies in immune rhesus serum were shown to bind to the surface membrane of 3 h schistosomula using an antibody-enzyme bridge technique ...	1975	1118189
permeability of ovarian follicles and capillaries in mice.	the permeability of ovarian capillaries and follicles in prepubertal and sexually mature (proestrus and metestrus) randomly bred swiss albino female mice (sch:ars ha icr strain) was studied by intravenous injection of either ferritin or horseradish peroxidase (hrp), followed by examination with light and electron microscopes. the study revealed that capillaries in the interstitial and perifollicular regions were provided with a continuous endothelium that had constant permeability characteristic ...	1975	1119413
increased penetration of horseradish peroxidase across the blood-brain barrier induced by metrazol seizures.		1975	1122393
lymphocyte differentiation and antibody synthesis in the secondary immune response of peroxidase stimulated lymph nodes of rat.	lymphocyte differentiation and specific antibody synthesis were studied in the course of the secondary immune response of horseradish-peroxidase immunized rat popliteal lymph nodes by electron microscopic immunocytochemistry. from primary immunization, mature plasma cells containing specific antibody remained as long as 10 months. antihorseradish peroxidase antibodies were localized in lymphocytic blast cells on day 3 after restimulation with antigen. in the subsequent days blast cells were stil ...	1975	1122527
an ultrastructural quantitative method for the evaluation of the permeability to horseradish peroxidase of cerebral cortex endothelial cells of the rat.	a quantitative method for the evaluation of the importance of pinocytotic phenomena in cerebral cortex capillaries is proposed. the method involved the counting of labeled "caveolae intracellulares" in endothelia cells with the use of horseradish peroxidase as a tracer molecule. a mean pinocytotic value per capillary is then computed. it is demonstrated that the value obtained after irradiation of the brain is higher than in control animals. this information may provide an ultrastructural quanti ...	1975	1122535
h2o2 release from human granulocytes during phagocytosis. i. documentation, quantitation, and some regulating factors.	the extinction of fluorescence of scopoletin during its oxidation by horseradish peroxidase (hpo) provides a highly sensitive and specific assay for small quantities of peroxide in solution. with this assay, the release of free h2o2 into the extracellular medium by phagocytizing human granulocytes has been documented and quantitated, and some of the regulating factors have been determined. under basal conditions granulocytes released less than 0.01 nmol/ml of h2o2 (2.5 x 10-6 polymorphonuclear l ...	1975	1123431
application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system.	immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. the immunoperoxidase bridge techniques from reaction products of high quality which c ...	1975	1127219
sympathetic preganglionic neurons of the cat spinal cord: horseradish peroxidase study.		1975	1131693
diffusion of horseradish peroxidase perfused through the lateral ventricle of the chick telencephalon.	horseradish peroxidase, perfused into the lateral ventricle of chick brain, freely and slowly diffuses through the cerebral extracellular spaces. the layer of astrocytic end-feet surrounding blood capillaries does not consitute a barrier to the tracer which permeates the basal lamina, diffuses between the pericytic cells and finally accumulates in the intercellular space beneath the tight junctions between contiguous endothelial cells. no evidence was found for transport by micropinocytotic vesi ...	1975	1131857
effects of contrast agents on the blood-brain barrier. an electron microscopic study.	to determine if contrast agents after the blood-brain barrier by opening tight junctions between endothelial cells and by increasing pinocytosis, the distribution of horseradish peroxidase (hrp) was evaluated after intracarotid injections of contrast media. hrp did not cross the endothelium of cerebral blood vessels. tight junctions remained closed. there was increased endothelial cell pinocytosis and swelling of astrocyte end-feet.	1975	1138267
the effect of portocaval anastomosis on the permeability to horseradish peroxidase of cerebral vessels of the rat.	the blood-brain barrier to horseradish peroxidase (hrp) was tested in rats with hepatic encephalopathy induced by portocaval anastomosis. an increased number of vesicles containing hrp was seen in endothelial cells. basement membranes of some vessels and the adjacent neuropil were filled with hrp. astrocytes showed swollen end-feet. the cytoplasm of astrocytes adjacent to hrp containing basement membranes was evenly filled with hrp. this phenomenon could be caused either by cellular hyperactivit ...	1975	1146553
some aspects of the organization of the thalamic reticular complex.	anatomical methods which depend upon the anterograde axonal transport of isotopically labeled neuronal proteins or the retrograde axonal transport of the enzyme, horseradish peroxidase, have been used to elucidate the relationships between the reticular complex and the dorsal thalamus and cerebral cortex. injections of tritiated amino acids in the dorsal thalamus or cerebral cortex in rats, cats and monkeys, show that as the bundles of thalamo-cortical and cortico-thalamic fibers joining a parti ...	1975	1150923
pathogenesis of acute arterial fat deposition in spontaneously hypertensive rats.	the selectively-bred substrains of spontaneously hypertensive rats with a greater vulnerability to vascular lesions rapidly developed arterial fat deposition within 1 or 2 weeks as well as a greater hypercholesterolemic response when fed on high fat cholesterol diet including 20% of suet, 5% of cholesterol and 2% of cholic acid. the ring-like arterial fat deposition at the branches of superior mesenteric arteries and cerebrobasal arteries, which was found to be good indices for the deposition of ...	1975	1152192
evidence for bidirectional dendritic transport of horseradish peroxidase.		1975	1155260
observations on cortical neurons retrogradely labeled with horseradish peroxidase.		1975	1155261
the appearance of dendrites of callosal and corticothalamic neurons in somatosensory cortex of immature rats demonstrated by horseradish peroxidase.		1975	1155262
absorption of intact protein by colonic epithelial cells of the rat.	colonic absorption of intact protein was examined in adult rats using histological and ultrastructural procedures. horseradish peroxidase was introduced into ligated colonic loops and retained therein for 5, 10, 20, or 30 minutes prior to excision of the loops, and their processing for microscopy. morphological evaluation revealed evidence of peroxidase absorption via pinocytosis. tracer particles were observed adherent to the mucosal border, in apical pinocytotic vesicles, in vesicles adjacent ...	1975	1155414
demonstration of a somatotopically organized projection onto the paramedian lobule and the anterior lobe from the lateral reticular nucleus: an experimental study with the horseradish peroxidase method.	using the retrograde axonal transport of horseradish peroxidase, the projection from the lateral reticular nucleus (nrl) to the cerebellar anterior lobe and paramedian lobule has been studied in 13 cats. both the anterior lobe and the paramedian lobule receive a somatotopically organized projection from the nrl. the projection to the paramedian lobule is nearly exclusively ipsilateral and originates mainly in the dorsal part of nrl, while the projection to the anterior lobe is bilateral (with ip ...	1975	1156873
the pontocerebellar projection onto the paramedian lobule in the cat: an experimental study with the use of horseradish peroxidase as a tracer.	horseradish peroxidase (hrp) was injected into cerebellar cortex of the paramedian lobule in 12 cats, and the ensuing distribution of labeled cells in the pontine nuclei was mapped in some detail. the cells in the pontine gray which give origin to fibers to the paramedian lobule lie together, in part in groups, and in part in columns. the columns are situated both medial and ventrolateral to the peduncle, as well as in the dorsolateral pontine nucleus. the projection is bilateral with a clearcut ...	1975	1156877
the projections of different morphological types of ganglion cells in the cat retina.	the central projections of the retinal ganglion cells of the cat were examined using the method of retrograde transport of horseradish peroxidase. peroxidase was injected into the lateral geniculate nucleus and into the superior colliculus by means of a recording micropipette. after injections at retinotopically homologous points in these two structures in separate animals, tha patterns of retinal ganglion cell labeling were compared. we found that there were three populations of ganglion cells: ...	1975	1159111
the projections of cells in different layers of the cat's visual cortex.	the projection of cells in different layers of several cortical visual areas in the cat were studied using the method of retrograde transport of horseradish peroxidase. injections of the enzyme were made through a recording micropipette, making it possible to localize the injection site by physiological criteria. we found that layer vi cells projected to the alteral geniculate nucleus, while a distinct population of cells in layer v projected to the superior colliculus. cells in layers ii and ii ...	1975	1159112
studies on the structure and permeability of the microvasculature in normal rat lymph nodes.	the structure and permeability of the microvasculature in normal rat lymph nodes was studied by regional perfusion techniques. the results indicated that characteristic vascular units supplied each cortical lobule of lymphatic tissue. numerous arteriovenous communications and venous sphincters innervated by unmyelinated nerve fibers were found in this vascular bed. these specialized vascular structures permitted regional control of blood flow through high endothelial venules. lymphocytes migrate ...	1975	1163637
impermeability of sertoli cell junctions to prolonged exposure to peroxidase.	the competence of the occluding junctional complexes between sertoli cell has been studied after continuous exposure of the testis to a high concentration of peroxidase for periods of 30 minutes to 4 hours. horseradish peroxidase in buffered saline solution (10 mg/ml) infused through an indwelling polyethelene tube implanted interstitially in the testis becomes widely distributed throughout the organ via the extensive system of lymphatic sinusoids. all the components of the testicular interstiti ...	1975	1163825
macromolecular transport by the middle ear and its lymphatic system.	the macromolecular transport in the middle ear mucosa of the guinea pig was investigated by means of light and electron microscopy using tracer substances such as evans blue, india ink, and horseradish peroxidase (hrp). hrp particles were readily taken up by the middle ear mucosa and eustachian tube. they were absorbed by all cell types, including ciliated, secretory and nonsecretory cells. the particles were first taken up by the pinocytotic vesicles and then transported into the intercellular ...	1975	1166775
immunoelectron microscopic identification and localization of streptococcus sanguis with peroxidase-labeled antibody: localization of streptococcus sanguis in intact dental plaque.	streptococcus sanguis has been localized ultrastructurally within intact dental plaque by means of an indirect technique which utilizes horseradish peroxidase-labeled antibody. the technique allows for complete diffusion of the reagents to all portions of the plaque specimens. control procedures can be carried out on serial sections of plaque with a bacterial composition similar to that of the experimental specimen. the 30-mum-thick sections can be examined in the light microscope to localize ar ...	1975	1167846
endogenous peroxidase: specific marker enzyme for tissues displaying growth dependency on estrogen.	data derived from a correlated morphological and biochemical study suggest the following: (a) estradiol-17beta, diethylstilbestrol, the estrogen antagonists nafoxidine (upjohn 11,000), and parke davis c1628 induce synthesis of an endogenous peroxidase in the epithelium of target tissues like the vagina, the cervix, the uterus, and in the acinar cells of the estrogen-dependent rat mammary tumor; (b) peroxidase is a "specific" secretory protein of the estrogen-sensitized uterine endometrium; (c) p ...	1975	1171106
permeability of rabbit corneal epithelium to horseradish peroxidase after the influence of benzalkonium chloride.	a tight barrier against permeation of horseradish peroxidase into the corneal epithelium exists at the corneal surface adjacent to the tear film. the present light and transmission electron microscopic study reveals that the cationic surfactant, benzalkonium chloride, which is commonly added to eye drops as a preservative, breaks down this barrier. lysis of the cell membranes was demonstrated, resulting in a leakage of the tracer into and underneath the superficial cells. the grade of cellular d ...	1975	1174396
magnocellular nuclei of the basal forebrain project to neocortex, brain stem, and olfactory bulb. review of some functional correlates.	horseradish peroxidase was injected into the neocortex of squirrel monkeys, rats, tree shrews and one opossum, in the brain stem of one squirrel monkey and rats, and in the olfactory bulb, the corpus vitreum or the vascular system of rats. following the cortical, brain stem and bulbar injections labeled cells were found (predominatly ipsilaterally) in the magnocellular nuclei of the basal forebrain: nucleus of the diagonal band, the magnocellular preoptic nucleus and nucleus basalis. these nucle ...	1975	1174977