Publications

a consensus linkage map for molecular markers and quantitative trait loci associated with economically important traits in melon (cucumis melo l.).	a number of molecular marker linkage maps have been developed for melon (cucumis melo l.) over the last two decades. however, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. in order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (qtl) analysis, increasing breeding e ...	2011	21797998
expression, subcellular localization, and cis-regulatory structure of duplicated phytoene synthase genes in melon (cucumis melo l.).	carotenoids perform many critical functions in plants, animals, and humans. it is therefore important to understand carotenoid biosynthesis and its regulation in plants. phytoene synthase (psy) catalyzes the first committed and rate-limiting step in carotenoid biosynthesis. while psy is present as a single copy gene in arabidopsis, duplicated psy genes have been identified in many economically important monocot and dicot crops. cmpsy1 was previously identified from melon (cucumis melo l.), but w ...	2011	21626149
cell wall polysaccharides of near-isogenic lines of melon (cucumis melo l.) and their inbred parentals which show differential flesh firmness or physiological behavior.	we characterized differences in cell wall material and polysaccharide structures, due to the quantitative trait loci associated with higher flesh firmness in a nonclimacteric near-isogenic line (nil) sc7-2, and with the climacteric behavior of the nil sc3-5-1, using their nonclimacteric inbred parentals, "piel de sapo" (ps) and pi 161375 (sc). ps was firmer and had a higher ripening index and greater hemicellulosic content than sc, with its lower wall material yield, and uronic acid, neutral sug ...	2011	21608971
analysis of expressed sequence tags generated from full-length enriched cdna libraries of melon.	melon (cucumis melo), an economically important vegetable crop, belongs to the cucurbitaceae family which includes several other important crops such as watermelon, cucumber, and pumpkin. it has served as a model system for sex determination and vascular biology studies. however, genomic resources currently available for melon are limited.	2011	21599934
carotene and novel apocarotenoid concentrations in orange-fleshed cucumis melo melons: determinations of β-carotene bioaccessibility and bioavailability.	muskmelons, both cantaloupe (cucumis melo reticulatus group) and orange-fleshed honeydew (c. melo inodorus group), a cross between orange-fleshed cantaloupe and green-fleshed honeydew, are excellent sources of β-carotene. although β-carotene from melon is an important dietary antioxidant and precursor of vitamin a, its bioaccessibility/bioavailability is unknown. we compared β-carotene concentrations from previously frozen orange-fleshed honeydew and cantaloupe melons grown under the same glassh ...	2011	21417375
ineffective topical treatment of vitiligo with cucumis melo extracts.		2011	21342177
extensive metabolic cross-talk in melon fruit revealed by spatial and developmental combinatorial metabolomics.	• variations in tissue development and spatial composition have a major impact on the nutritional and organoleptic qualities of ripe fleshy fruit, including melon (cucumis melo). to gain a deeper insight into the mechanisms involved in these changes, we identified key metabolites for rational food quality design. • the metabolome, volatiles and mineral elements were profiled employing an unprecedented range of complementary analytical technologies. fruits were followed at a number of time points ...	2011	21275993
codominant pcr-based markers and candidate genes for powdery mildew resistance in melon (cucumis melo l.).	powdery mildew caused by podosphaera xanthii is a major disease in melon crops, and races 1, 2, and 5 of this fungus are those that occur most frequently in southern europe. the genotype tgr-1551 bears a dominant gene that provides resistance to these three races of p. xanthii. by combining bulked segregant analysis and amplified fragment length polymorphisms (aflp), we identified eight markers linked to this dominant gene. cloning and sequencing of the selected aflp fragments allowed the develo ...	2011	21243332
chlorophenoxyacetic acid and chloropyridylphenylurea accelerate translocation of photoassimilates to parthenocarpic and seeded fruits of muskmelon (cucumis melo).	we compared the effect of p-chlorophenoxyacetic acid (p-cpa) and 1-(2-chloro-4-pyridyl)-3-phenylurea (cppu) on parthenocarpic and seeded muskmelon (cucumis melo) fruits in regards to fruit development and the transport of photoassimilates from leaves exposed to ¹⁴co₂ to the developing fruits. ten days after anthesis (daa), the fresh weight, total ¹⁴c-radioactivity and contents of ¹⁴c-sucrose and ¹⁴c-fructose were higher in the cppu-induced parthenocarpic fruits than in seeded fruits. however, at ...	2011	21168241
determination of the melon chloroplast and mitochondrial genome sequences reveals that the largest reported mitochondrial genome in plants contains a significant amount of dna having a nuclear origin.	the melon belongs to the cucurbitaceae family, whose economic importance among vegetable crops is second only to solanaceae. the melon has a small genome size (454 mb), which makes it suitable for molecular and genetic studies. despite similar nuclear and chloroplast genome sizes, cucurbits show great variation when their mitochondrial genomes are compared. the melon possesses the largest plant mitochondrial genome, as much as eight times larger than that of other cucurbits.	2011	21854637
muskmelon embryo rescue techniques using in vitro embryo culture.	among the major cucurbit vegetables, melon (cucumis melo) has one of the greatest polymorphic fruit types and botanical varieties. some melon fruits have excellent aroma, variety of flesh colors, deeper flavor, and more juice compared to other cucurbits. despite numerous available melon cultivars, some of them are exceedingly susceptible to several diseases. the genetic background carrying the genes for tolerance and/or resistance for those diseases is found in wild melon landraces. unfortunatel ...	2011	21207265
aminopyralid soil residues affect rotational vegetable crops in florida.	bahiagrass (paspalum notatum flueggé) is a poor host of several soilborne pests of vegetable crops; therefore vegetable crops are commonly grown in a rotation with bahiagrass pastures in florida. the herbicide aminopyralid provides foliar and soil residual weed control and increases forage production in bahiagrass pastures; however, the soil residual activity of aminopyralid makes carryover injury likely in subsequent sensitive vegetable crops. field research was conducted to determine the sensi ...	2011	21413141
molecular cloning and expression of cucumisin (cuc m 1), a subtilisin-like protease of cucumis melo in escherichia coli.	oral allergy syndrome resulted from plant-derived foods is frequent among adults. allergy to melon (cucumis melo) is one of the most frequent fruit allergies in iran. three different major allergens have been found in cucumis melo that cuc m 1 (cucumisin) has been identified as the major allergen of melon. cucumisin is an alkaline serine protease that it is found as a 78kda protein in precursor form. the aim of this study was production of recombinant cuc m 1 in escherichia coli (e. coli) cells ...	2011	21173569
characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: squash leaf curl virus and watermelon chlorotic stunt virus.	squash leaf curl virus (slcv) and watermelon chlorotic stunt virus (wmcsv) are cucurbit-infecting bipartite begomoviruses. both viruses are found in the eastern mediterranean basin but the effects of dual infection of both viruses on melon (cucumis melo l.) have not been described. 'arava' melon plants were inoculated in the greenhouse, using whiteflies, with either slcv, wmcsv, or both. control plants were exposed to nonviruliferous whiteflies or not exposed at all. following inoculation, plant ...	2011	21219130
sucrose transporter plays a role in phloem loading in cmv-infected melon plants that are defined as symplastic loaders.	based on the high density of plasmodesmata interconnecting the intermediary cells and their neighboring phloem parenchyma or bundle-sheath cells, and based on the insensitivity to the sucrose transport inhibitor p-chloromercuribenzenesulfonic acid (pcmbs), cucurbits have been concluded to be symplastic loaders. in the present study, we identified and characterized the full-length sequence of sucrose transporter gene (cmsut1) from melon (cucumis melo l. cv. hale's best jumbo). in vitro experiment ...	2011	21241389
fungal microbiota from rain water and pathogenicity of fusarium species isolated from atmospheric dust and rainfall dust.	in order to determine the presence of fusarium spp. in atmospheric dust and rainfall dust, samples were collected during september 2007, and july, august, and october 2008. the results reveal the prevalence of airborne fusarium species coming from the atmosphere of the south east coast of spain. five different fusarium species were isolated from the settling dust: fusarium oxysporum, f. solani, f. equiseti, f. dimerum, and f. proliferatum. moreover, rainwater samples were obtained during signifi ...	2011	20820862
a melon pulp concentrate rich in superoxide dismutase reduces stress proteins along the gastrointestinal tract of pigs.	a melon (cucumis melo lc.) pulp concentrate (mpc) rich in superoxide dismutase (sod) activity was tested for its ability to decrease stress protein expressions along the gastrointestinal tract in a swine model.	2011	20558040
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.	fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...	2011	23961146
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.	microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...	2011	23431109
the genetic basis of resistance to downy mildew in cucumis spp.--latest developments and prospects.	downy mildew, caused by the oomycete pathogen pseudoperonospora cubensis, is one of the most destructive diseases of cucumber (cucumis sativus l.) and muskmelon (c. melo l.). although the process of pathogenesis is well understood, there are few disease control options available. the development and deployment of resistant cultivars is generally considered to be the best approach to control downy mildew. the recently completed sequencing of the cucumber genome provides a great opportunity for re ...	2011	21318301
distinct colonization patterns and cdna-aflp transcriptome profiles in compatible and incompatible interactions between melon and different races of fusarium oxysporum f. sp. melonis.	fusarium oxysporum f. sp. melonis snyd. & hans. (fom) causes fusarium wilt, the most important infectious disease of melon (cucumis melo l.). the four known races of this pathogen can be distinguished only by infection on appropriate cultivars. no molecular tools are available that can discriminate among the races, and the molecular basis of compatibility and disease progression are poorly understood. resistance to races 1 and 2 is controlled by a single dominant gene, whereas only partial polyg ...	2011	21338485
watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan.	the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ...	2011	21399920
infectious clones of tomato leaf curl palampur virus with a defective dna b and their pseudo-recombination with tomato leaf curl new delhi virus.	abstract: background: tomato leaf curl palampur virus (tolcpmv) is a bipartite begomovirus which has been reported from india and iran but infectious clones have not been obtained. we have previously shown the association of zucchini yellow mosaic virus (zymv), a potyvirus, with severe leaf curl disease of muskmelon in pakistan. however, the severity of symptoms in the field and yield losses led us to believe that some other agent, such as a begomovirus, could be associated with the disease. res ...	2011	21496256
inactivation of snt2, a bah/phd-containing transcription factor, impairs pathogenicity and increases autophagosome abundance in fusarium oxysporum.	the soil-borne, asexual fungus fusarium oxysporum f.sp. melonis (fom) is a causal agent of muskmelon wilt disease. the current study focused on the most virulent race of fom-race 1,2. the tagged mutant d122, generated by agrobacterium tumefaciens-mediated transformation, caused the delayed appearance of initial wilt disease symptoms, as well as a 75% reduction in pathogenicity. d122 was impaired in the gene product homologous to the snt2-like transcription factor of schizosaccharomyces pombe. in ...	2011	21535351
ecology and management of whitefly-transmitted viruses of vegetable crops in florida.	a variety of fresh market vegetables, including watermelon and tomato are economically important crops in florida. whitefly-transmitted squash vein yellowing virus (sqvyv) was first identified in squash and watermelon in florida in 2005 and shown to cause a severe decline of watermelon vines as crops approach harvest. florida is most economically impacted by sqvyv, although the virus has been detected more recently in indiana and south carolina. the origin and evolutionary history of sqvyv, one ...	2011	21549768
light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, opisthorchis viverrini (platyhelminthes, digenea).	eggs of most species digenean flukes hatch in the external environment to liberate larvae that seek and penetrate a snail intermediate host. those of the human liver flukes, opisthorchis viverrini, hatch within the gastrointestinal canal of their snail hosts. while adult parasites are primarily responsible for the pathology in cases of human opisthorchiasis, their eggs also contribute by inducing granulomata and in serving as nidi for gallstone formation. in view of the peculiar biology of o. vi ...	2011	21786067
olpidium bornovanus-mediated germination of ascospores of monosporascus cannonballus: a host-specific rhizosphere interaction.	monosporascus cannonballus, a host-specific root-infecting ascomycete, is the causal agent of a destructive disease of melon (cucumis melo l.) known as vine decline. ascospores germinate only in the rhizosphere of melon plants growing in field soil. however, no germination occurs in the rhizosphere of melon plants if the field soil is heated to temperatures >50-¦c prior to infestation with ascospores. this observation suggested that germination is mediated by one or more heat-sensitive members o ...	2011	21675923
histological study of organogenesis in cucumis melo l. after genetic transformation: why is it difficult to obtain transgenic plants?	melon (cucumis melo l.) is widely considered as a recalcitrant species for genetic transformation. in this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. the highest regeneration rate (1.13-á-¦-á0.02 plants per explant) was obtained using cotyledon explants of the 'v+®drantais' genotype on murashige and skoog (ms) medium supplemented with 0.2-ámg/l 6-benzylaminopurine (bap) and 0.2-ámg/l ...	2011	21706229
Low genetic diversity of Squash vein yellowing virus in wild and cultivated cucurbits in the U.S. suggests a recent introduction.	Squash vein yellowing virus (SqVYV) isolates were collected from cultivated and weedy cucurbits representing major hosts and locations in the U.S. and analyzed to better understand the diversity and population structure. No differences in symptoms were observed in field-collected isolate source plants or subsequently inoculated greenhouse plants, and the complete genome of an SqVYV isolate from a wild cucurbit host (smellmelon, Cucumis melo var. dudaim) was highly similar (99.4% nucleotide ident ...	2011	22142477
a novel member of the genus nepovirus isolated from cucumis melo in japan.	an unusual virus was isolated from a japanese cucumis melo cv. prince melon plant showing mild mottling of the leaves. the virus had a broad experimental host range including at least 19 plant species in five families, with most infected plants showing no symptoms on inoculated and uninoculated systemically infected leaves. the virus particles were spherical, approximately 28 nm in diameter, and the coat protein (cp) had an apparent molecular mass of about 55 kda. the virus possessed a bi-partit ...	2011	20955084
arabidopsis suc1 loads the phloem in suc2 mutants when expressed from the suc2 promoter.	active loading of sucrose into phloem companion cells (ccs) is an essential process in apoplastic loaders, such as arabidopsis or tobacco (nicotiana sp.), and is even used by symplastic loaders such as melon (cucumis melo) under certain stress conditions. reduction of the amount or complete removal of the transporters catalysing this transport step results in severe developmental defects. here we present analyses of two arabidopsis lines, suc2-4 and suc2-5, that carry a null allele of the suc2 g ...	2011	22021573
expression and large-scale production of the biochemically active human tissue-plasminogen activator in hairy roots of oriental melon (cucumis melo).	human tissue-plasminogen activator (t-pa) is a thrombolytic protein that plays an active role in dissolving fibrin clots by fibrinolysis and in activating plasminogen to plasmin in blood vessels. t-pa and synthetic t-pa (st-pa) genes were expressed as enzymatically active form in hairy roots of oriental melon (cucumis melo l. cv. geumssaragi-euncheon) infected by agrobacterium rhizogenes. the insertion of the t-pa genes in genomic dna of transgenic hairy roots was verified by pcr. the presence a ...	2011	22018738
Quantitative analysis of mycoflora on commercial domestic fruits in Japan.	A comprehensive and quantitative analysis of the mycoflora on the surface of commercial fruit was performed. Nine kinds of fruits grown in Japan were tested. Overall fungal counts on the fruits ranged from 3.1 to 6.5 log CFU/g. The mean percentages of the total yeast counts were higher than those of molds in samples of apples, Japanese pears, and strawberries, ranging from 58.5 to 67.0%, and were lower than those of molds in samples of the other six fruits, ranging from 9.8 to 48.3%. Cladosporiu ...	2011	21902918
biological and molecular characterization of two distinct tomato strains of cucumber mosaic virus based on complete rna-3 genome and subgroup specific diagnosis.	cucumber mosaic virus (cmv) is one of the most important viral pathogen infecting several plant species in india. five isolates of cmv obtained from cucumber, muskmelon, tobacco and tomato from distinct geographical locations in india were analysed based on host-reactions and genome sequence. the majority of the isolates were very similar and only two isolates, tfr-in and tss-in showed distinct symptoms in tomato and high sequence diversity (77.8%) in coat protein (cp) gene. tfr-in was isolated ...	2011	23637513
taxonomic significance of trichomes micromorphology in cucurbits.	studies on trichomes micromorphology using scanning electron microscope (sem) were undertaken in 23 species with one variety under 13 genera of the family cucurbitaceae (viz., benincasa hispida (thunb.) cogn., citrullus lanatus (thunb.) matsum. & nakai, cucumis melo var. agrestis naudin, cucumis sativus l., diplocyclos palmatus (l.) c. jeffrey, edgaria dargeelingensis c.b. clarke, gynostemma burmanicum king ex chakr., gynostemma pentaphyllum (thunb.) makino, gynostemma pubescens (gagnep.) c.y. w ...	2011	23961108
effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits.	the effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (averrhoa carambola l.), mango (mangifera indica l.), papaya (carica papaya l.), muskmelon (cucumis melo l.), and watermelon citruluss lanatus (thunb.) were investigated. significant (p < 0.05) differences, for the amounts of total phenolic compounds (tpc), were found between the fresh and freeze-dried fruit samples, except muskmelon. there was no significant (p > 0.05) change ...	2011	21845104
[construction of a melon genetic map with fruit and seed qtls].	a genetic map of melon was constructed using 143 f2 population developed from a cross between two distant lines ano2 of japan and hami melon k413. the map contains 12 linkage groups and 142 markers, including 121 aflps, 16 ssrs, 3 stss, 2 trait markers and covers 1 014.2 cm. composite interval mapping (cim) method was used to detect qtls involved in melon fruit and seed traits: fruit length (fl), fruit width (fw), fruit shape (length/width, fs), centre sugar (cs), edge sugar (es), flesh texture ...	2011	22207387
reduction of latent infection and enhancement of disease resistance in muskmelon by preharvest application of harpin.	latent infection was analyzed when harpin at 50 mg/l was sprayed on muskmelon 4 times at four different stages: florescence, young fruit, fruit enlarging, and netting periods. results showed that the latent infection was significantly lower in sprayed muskmelons than in the control fruit. meanwhile, the activities of peroxidase, phenylalanine ammonia-lyase, β-1,3-glucanase, and chitinase increased significantly in the fruit treated with harpin. it also resulted in an increase of contents of tota ...	2011	22050047
phenotypic stability of hybrids of gália melon in rio grande do norte state, brazil.	the objectives of this study were to determine the importance of simple and complex components of the interaction genotype × environment and to evaluate the adaptability and stability of gália melon hybrids. nine hybrids were tested in twelve environments of rio grande norte state from 2000 to 2001. the experiments were carried out in a randomized complete block design with three replications. the statistical methods of toler and burrows, wricke and ammi (additive main effect and multiplicative ...	2011	21971597
[effects of gamma-aminobutyric acid on the photosynthesis and chlorophyll fluorescence parameters of muskmelon seedlings under hypoxia stress].	by the method of hydroponic culture, this paper studied the effects of exogenous gamma-aminobutyric acid (gaba) on the photosynthetic pigment contents, photosynthesis, and chlorophyll fluorescence parameters of muskmelon seedlings under hypoxia stress. hypoxia stress induced a significant decrease of photosynthetic pigment contents, resulting in the decrease of photosynthesis. applying gaba could significantly increase the photosynthetic pigment contents, net photosynthetic rate (p(n)), stomatal ...	2011	21774324
exogenous calcium affects nitrogen metabolism in root-zone hypoxia-stressed muskmelon roots and enhances short-term hypoxia tolerance.	we investigated the effects of short-term root-zone hypoxic stress and exogenous calcium application or deficiency in an anoxic nutrient solution on nitrogen metabolism in the roots of the muskmelon cultivar xiyu no. 1. seedlings grown in the nutrient solution under hypoxic stress for 6d displayed significantly reduced plant growth and soluble protein concentrations. however, no₃⁻ uptake rate and activities of nitrate reductase and glutamate synthase were significantly increased. we also found h ...	2011	21458885
cantaloupe melon-like stomach.		2012	22154414
[nondestructive measurement of sugar content of hami melon based on diffuse reflectance hyperspectral imaging technique].	the research on nondestructive test for detecting the sugar content of hami melon by the technology of hyperspectral imaging was put forward. the research used the hyperspectral imaging system to get the diffuse reflective spectrum information (400 - 1 000 nm) of anilox class hami melon sugar content, chose effective whole wavelength (500 - 820 nm)to do the modeling regression analysis the sugar content of hami melon. the research compared the correction method of msc and snv, and also compared ...	2012	23387186
verification of aroma profiles of jiashi muskmelon juice characterized by odor activity value and gas chromatography-olfactometry/detection frequency analysis: aroma reconstitution experiments and omission tests.	to verify the aroma profile of jiashi muskmelon previously identified by gas chromatography-olfactometry/detection frequency analysis (gc-o/dfa) and odor activity value (oav) calculation, the synthetic blends of odorants (aroma models) were prepared and then were compared with the original jiashi muskmelon juice aroma using quantitative descriptive analysis (qda) and electronic nose analysis (ena), respectively. qda and ena both indicated that the model solution derived from oav calculation more ...	2012	22970770
high efficiency joint cze determination of sugars and acids in vegetables and fruits.	in this work, an improved ce method for the medium-throughput determination of main organic acids (oxalate, malate, citrate), the amino acid glutamate and the sugars fructose, glucose and sucrose in several food matrices is described. these compounds have been identified as key components in the taste intensity of fruit and vegetable crops. using a running buffer with 20 mm 2,6-pyridine dicarboxylic acid ph 12.1 and 0.1% hexadimethrine bromide, replacing it every 5 h to avoid ph decrease, and op ...	2012	22887163
pharmacological basis for the medicinal use of muskmelon base (pedicellus melo.) for abdominal distention and constipation.	muskmelon base (pedicellus melo.) has a long history (ming dynasty) as a chinese traditional medicine. according to traditional use, it was prepared as rectal suppositories for treating abdominal distention and constipation. the present study was carried out on the pharmacological basis for the medicinal use of muskmelon base.	2012	22543170
identification of aroma-active compounds in jiashi muskmelon juice by gc-o-ms and oav calculation.	to identify aromatic compounds in jiashi melon juice, gas chromatography-mass spectrometry-olfactometry (gc-ms-o) analysis was used. odor activity values (oavs) were also calculated on the basis of the qualitative and quantitative analysis of volatile compounds. results showed that 42 volatiles were identified, among which 4 compounds, namely, diethyl carbonate, isophorone, 2-butoxyethyl acetate, and menthol, were identified or tentatively identified for the first time as volatiles in melon frui ...	2012	22497266
enantioselective determination of triazole fungice tetraconazole by chiral high-performance liquid chromatography and its application to pharmacokinetic study in cucumber, muskmelon, and soils.	a simple chiral high-performance liquid chromatography method with diode array detector was developed and validated for stereoselective determination of tetraconazole enantiomers in cucumber, muskmelon, and soils. good separation was achieved at 20°c using cellulose tris-(4-methylbenzoate) as chiral stationary phase, a mixture of n-hexane and ethanol (90:10) as mobile phase at a flow rate of 0.8 ml/min. the assay method was linear over a range of concentrations (0.5-50 μg/ml) and the mean recove ...	2012	22278951
phloem-mobile aux/iaa transcripts target to the root tip and modify root architecture.	in plants, the phloem is the component of the vascular system that delivers nutrients and transmits signals from mature leaves to developing sink tissues. recent studies have identified proteins, mrna, and small rna within the phloem sap of several plant species. it is now of considerable interest to elucidate the biological functions of these potential long-distance signal agents, to further our understanding of how plants coordinate their developmental programs at the whole-plant level. in thi ...	2012	22925478
medieval emergence of sweet melons, cucumis melo (cucurbitaceae).	sweet melons, cucumis melo, are a widely grown and highly prized crop. while melons were familiar in antiquity, they were grown mostly for use of the young fruits, which are similar in appearance and taste to cucumbers, c. sativus. the time and place of emergence of sweet melons is obscure, but they are generally thought to have reached europe from the east near the end of the 15th century. the objective of the present work was to determine where and when truly sweet melons were first developed.	2012	22648880
assessment of root uptake and systemic vine-transport of salmonella enterica sv. typhimurium by melon (cucumis melo) during field production.	among melons, cantaloupes are most frequently implicated in outbreaks and surveillance-based recalls due to salmonella enterica. there is limited but compelling evidence that associates irrigation water quality as a significant risk of preharvest contamination of melons. however, the potential for root uptake from water and soil and subsequent systemic transport of salmonella into melon fruit is uncharacterized. the aim of this work was to determine whether root uptake of s. enterica results in ...	2012	22824339
plectosphaerella species associated with root and collar rots of horticultural crops in southern italy.	plectosphaerella cucumerina, most frequently encountered in its plectosporium state, is well known as a pathogen of several plant species causing fruit, root and collar rot, and collapse. it is considered to pose a serious threat to melon (cucumis melo) production in italy. in the present study, an intensive sampling of diseased cucurbits as well as tomato and bell pepper was done and the fungal pathogens present on them were isolated. phylogenetic relationships of the isolates were determined t ...	2012	23105152
cucumis melo microrna expression profile during aphid herbivory in a resistant and susceptible interaction.	aphis gossypii resistance in melon (cucumis melo) is due to the presence of a single dominant virus aphid transmission (vat) gene belonging to the nucleotide-binding site leucine-rich repeat family of resistance genes. significant transcriptional reprogramming occurs in vat(+) plants during aphid infestation as metabolism shifts to respond to this biotic stress. micrornas (mirnas) are involved in the regulation of many biotic stress responses. the role of mirnas was investigated in response to a ...	2012	22375710
the fate of murine norovirus and hepatitis a virus during preparation of fresh produce by cutting and grating.	human noroviruses and hepatitis a virus (hav) are commonly associated with outbreaks occurring in restaurant establishments and catered events. food handlers are major contributing factors to foodborne illnesses initiated in the kitchen setting. in this study, transfer of hav and murine norovirus (mnv-1), a human norovirus surrogate, between produce (cucumbers, strawberries, tomatoes, cantaloupes, carrots, and honeydew melons) and common kitchen utensils (graters and knives) was investigated. th ...	2012	23412721
expression of small rna in aphis gossypii and its potential role in the resistance interaction with melon.	the regulatory role of small rnas (srnas) in various biological processes is an active area of investigation; however, there has been limited information available on the role of srnas in plant-insect interactions. this study was designed to identify srnas in cotton-melon aphid (aphis gossypii) during the vat-mediated resistance interaction with melon (cucumis melo).	2012	23173035
microarray analysis shows that recessive resistance to watermelon mosaic virus in melon is associated with the induction of defense response genes.	resistance to watermelon mosaic virus (wmv) in melon (cucumis melo l.) accession tgr-1551 is characterized by a significant reduction in virus titer, and is inherited as a recessive, loss-of-susceptibility allele. we measured virus rna accumulation in tgr-1551 plants and a susceptible control ('tendral') by real-time quantitative polymerase chain reaction, and also profiled the expression of 17,443 unigenes represented on a melon microarray over a 15-day time course. the virus accumulated to hig ...	2012	21970693
comparative morphology of minute intestinal fluke eggs that can occur in human stools in the republic of korea.	the egg morphology of minute intestinal flukes (mif) that can occur as human infections in the republic of korea, i.e., metagonimus yokogawai, m. miyatai, m. takahashii, heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus, stictodora fuscata, pygidiopsis summa, and gymnophalloides seoi, was studied in comparison with clonorchis sinensis. the adult worms were obtained from residents of endemic areas, and their intrauterine eggs were studied and measured using light microscopy; t ...	2012	22949747
cholecystolithiasis is associated with clonorchis sinensis infection.	the objective of this study was to analyze gallbladder stones for direct evidence of a relationship between clonorchis sinensis infection and gallbladder stones formation.	2012	22905137
bacterial mixture from greenhouse soil as a biocontrol agent against root-knot nematode, meloidogyne incognita, on oriental melon.	the biological control efficacy of a greenhouse soil bacterial mixture of lactobacillus farraginis, bacillus cereus, and bacillus thuringiensis strains with antinematode activity was evaluated against the root-knot nematode meloidogyne incognita. two control groups planted in soil drenched with sterile distilled water or treated with the broadspectrum carbamate pesticide carbofuran were used for comparison. the results suggest that the bacterial mixture is effective as a biocontrol agent against ...	2012	22297227
validity of using recombinant melon profilin, cuc m 2, for diagnosis of melon allergy.	allergy is a clinical disorder affecting humans worldwide. allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the natural ones. cuc m 2 (profilin), one of the most important allergens of melon (cucumis melo), has been cloned and wa ...	2012	26989703
intestinal cell targeting of a stable recombinant cu-zn sod from cucumis melo fused to a gliadin peptide.	the mrna encoding full length chloroplastic cu-zn sod (superoxide dismutase) of cucumis melo (cantaloupe melon) was cloned. this sequence was then used to generate a mature recombinant sod by deleting the first 64 codons expected to encode a chloroplastic peptide signal. a second hybrid sod was created by inserting ten codons to encode a gliadin peptide at the n-terminal end of the mature sod. taking account of codon bias, both recombinant proteins were successfully expressed and produced in esc ...	2012	22426094
melon rna interference (rnai) lines silenced for cm-eif4e show broad virus resistance.	efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. recently, the cloning and characterization of recessive resistance genes from different plant species has pointed towards eukaryotic translation initiation factors (eif) of the 4e family a ...	2012	22309030
root transcriptional responses of two melon genotypes with contrasting resistance to monosporascus cannonballus (pollack et uecker) infection.	monosporascus cannonballus is the main causal agent of melon vine decline disease. several studies have been carried out mainly focused on the study of the penetration of this pathogen into melon roots, the evaluation of symptoms severity on infected roots, and screening assays for breeding programs. however, a detailed molecular view on the early interaction between m. cannonballus and melon roots in either susceptible or resistant genotypes is lacking. in the present study, we used a melon oli ...	2012	23134692
genetic diversity among melon accessions (cucumis melo) from turkey based on ssr markers.	melon (cucumis melo) is an important vegetable crop in turkey, where it is grown in many regions; the most widely planted lines are local winter types belonging to the var. inodorous. we examined 81 melon genotypes collected from different provinces of turkey, compared with 15 reference melon genotypes obtained from inra/france, to determine genetic diversity among turkish melons. twenty polymorphic primers were used to generate the ssr markers. pcr amplification was performed and electrophoresi ...	2012	23315810
dna fingerprinting of chinese melon provides evidentiary support of seed quality appraisal.	melon, cucumis melo l. is an important vegetable crop worldwide. at present, there are phenomena of homonyms and synonyms present in the melon seed markets of china, which could cause variety authenticity issues influencing the process of melon breeding, production, marketing and other aspects. molecular markers, especially microsatellites or simple sequence repeats (ssrs) are playing increasingly important roles for cultivar identification. the aim of this study was to construct a dna fingerpri ...	2012	23285039
anti-hyperlipidemic activity of cucumis melo fruit peel extracts in high cholesterol diet induced hyperlipidemia in rats.	abstract cucumis melo linn. (cucurbitaceae) fruits have been used, traditionally in indian traditional system of medicine, for the treatment of various disorders such as liver tonic, cardioprotective, antidiabetic, antiobesity, etc. the aim of the present study was to investigate the possible anti-hyperlipidemic activity of cucumis melo fruit peel (cmfp) methanolic and aqueous extract in high cholesterol diet induced hyperlipidemia in rats. treatment with cmfp methanolic and aqueous extract show ...	2012	23023565
electronic detection of drechslera sp. fungi in charentais melon ( cucumis melo naudin) using carbon-nanostructure-based sensors.	the development of chemical sensor technology in recent years has stimulated an interest regarding the use of characteristic volatiles and odors as a rapid and early indication of deterioration in fruit quality. the fungal infestation by drechslera sp. in melons is a severe problem, and we demonstrate that electronic sensors based on carbon nanostructures are able to detect the presence of these fungi in melon. the responses of sensor conductance g and capacitance c at 27 khz were measured and u ...	2012	23003336
crystal structure of cucumisin, a subtilisin-like endoprotease from cucumis melo l.	cucumisin is a plant serine protease, isolated as an extracellular glycoprotein from the melon fruit cucumis melo l. var. prince. cucumisin is composed of multiple domain modules, including catalytic, protease-associated, and fibronectin-iii-like domains. the crystal structure of cucumisin was determined by the multiwavelength anomalous dispersion method and refined at 2.75å resolution. a structural homology search indicated that the catalytic domain of cucumisin shares structural similarity wit ...	2012	22841692
the genome of melon (cucumis melo l.).	we report the genome sequence of melon, an important horticultural crop worldwide. we assembled 375 mb of the double-haploid line dhl92, representing 83.3% of the estimated melon genome. we predicted 27,427 protein-coding genes, which we analyzed by reconstructing 22,218 phylogenetic trees, allowing mapping of the orthology and paralogy relationships of sequenced plant genomes. we observed the absence of recent whole-genome duplications in the melon lineage since the ancient eudicot triplication ...	2012	22753475
transcriptome sequencing for snp discovery across cucumis melo.	melon (cucumis melo l.) is a highly diverse species that is cultivated worldwide. recent advances in massively parallel sequencing have begun to allow the study of nucleotide diversity in this species. the sanger method combined with medium-throughput 454 technology were used in a previous study to analyze the genetic diversity of germplasm representing 3 botanical varieties, yielding a collection of about 40,000 snps distributed in 14,000 unigenes. however, the usefulness of this resource is li ...	2012	22726804
analytical traceability of melon (cucumis melo var reticulatus): proximate composition, bioactive compounds, and antioxidant capacity in relation to cultivar, plant physiology state, and seasonal variability.	two morphologically different cultivars of italian melons (baggio and giusto) were characterized considering samples harvested in different times, at the beginning (bpp) and at the end of the physiological plant production period (epp). proximate composition, protein, minerals, ph, phenolic content, antioxidant capacity, ascorbic acid, carotenoids, condensed tannins, and flavonoids were measured, showing a significant decrease in epp samples (phenolics, antioxidant capacity, condensed tannins, a ...	2012	22583041
isolation and characterization of a muskmelon cdna encoding lycopene beta-cyclase.	lycopene beta-cyclase (lcy-b) is thought to play a critical role in beta-carotene synthesis in fruit. a full-length cdna clone encoding lycopene beta-cyclase was isolated from muskmelon (cucumis melo l.) by rt-pcr and race. the clone, designated cmlcyb1, contains 1871 nucleotides, with an open reading frame of 1512 nucleotides. the deduced 504-amino-acid sequence showed high identities with other plant lycopene beta-cyclases. real time quantitative rt-pcr analysis indicated that cmlcyb1 was expr ...	2012	22575730
diversity in expression of phosphorus (p) responsive genes in cucumis melo l.	phosphorus (p) is a major limiting nutrient for plant growth in many soils. studies in model species have identified genes involved in plant adaptations to low soil p availability. however, little information is available on the genetic bases of these adaptations in vegetable crops. in this respect, sequence data for melon now makes it possible to identify melon orthologues of candidate p responsive genes, and the expression of these genes can be used to explain the diversity in the root system ...	2012	22536378
effect of household and industrial processing on the levels of pesticide residues and degradation products in melons.	two varieties of melons (cucumis melo) were treated with two fungicides (carbendazim and maneb) and four insecticides (acetamiprid, cyromazin, imazalil and thiamethoxam) to quantify the effect of household processing on the pesticide residues. to ensure sufficiently high levels of residues in flesh and peel, the most concentrated formulations were applied observing good agricultural practice. the peeling step decreased the concentration of pesticide residues for maneb, imazalil and acetamiprid b ...	2012	22489844
co-mapping studies of qtls for fruit acidity and candidate genes of organic acid metabolism and proton transport in sweet melon (cucumis melo l.).	sweet melon cultivars contain a low level of organic acids and, therefore, the quality and flavor of sweet melon fruit is determined almost exclusively by fruit sugar content. however, genetic variability for fruit acid levels in the cucumis melo species exists and sour fruit accessions are characterized by acidic fruit ph of <5, compared to the sweet cultivars that are generally characterized by mature fruit ph values of >6. in this paper, we report results from a mapping population based on re ...	2012	22406955
1-methylcyclopropene effects on temporal changes of aroma volatiles and phytochemicals of fresh-cut cantaloupe.	orange-fleshed cantaloupe melons have intense aroma and flavor but are very perishable during storage life. fresh-cut processing enhances ethylene-mediated quality losses. post-cutting 1-methylcyclopene (1-mcp) application to fresh-cut cantaloupe was evaluated for its effects on quality attributes, phytochemical content and aroma volatiles.	2013	22821412
isolation, characterization, and expression analysis of cmmlo2 in muskmelon.	the full-length cdna sequence of the mlo gene was cloned via smart-race-pcr from muskmelon (cucumis melo l.), and was designated as cmmlo2 (genbank accession no. fj713542). the gene is 1,710 bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. localization analysis in onion epidermal cells showed that cmmlo2-gfp is localized in the plasma membrane. the expression of cmmlo2 gene was analyzed in melon leaf infected with po ...	2013	23238921
identification of early response genes to salt stress in roots of melon (cucumis melo l.) seedlings.	in order to better understand the mechanisms by which muskmelons (cucumis melo l.) respond to salt stress, a cdna library was constructed using suppression subtractive hybridization (ssh) from the root tissue of a salt-tolerant melon cultivar, bingxuecui. a total of 339 clones were sequenced from the ssh library, leading to 312 high quality expressed sequence tags (ests), with an average size of 450 bp; representing 262 uni-ests comprising 29 contigs and 233 singletons. blast analysis of the ded ...	2013	23212618
structural analysis of the alcohol acyltransferase protein family from cucumis melo shows that enzyme activity depends on an essential solvent channel.	alcohol acyltransferases (aat) play a key role in ester biosynthesis. in cucumis melo var. cantalupensis, aats are encoded by a gene family of four members (cmaat1-4). cmaat1, cmaat3 and cmaat4 are capable of synthesizing esters, with cmaat1 the most active. cmaat2 is inactive and has an ala268 residue instead of a threonine which is present in all other active aats, although the role of this residue is still unclear. the present work aims to understand the molecular mechanism involved in ester ...	2013	23311868
correlation and path coefficient analyses in sweet melon (cucumis melo var. aegyptiacus l.) under irrigated and drought conditions.	genotypic correlation and path analyses were carried out for growth, yield and fruit quality traits in 13 sweet melon genotypes collected from different places in egypt. seeds of these melon populations were sown under irrigated and drought stress conditions. the analysis of variance for the studied traits showed that the differences among genotypes were highly significant for all studied traits under irrigation and drought stress. under irrigated conditions, total yield per plant was positively ...	2013	24505983
high presence/absence gene variability in defense-related gene clusters of cucumis melo.	changes in the copy number of dna sequences are one of the main mechanisms generating genome variability in eukaryotes. these changes are often related to phenotypic effects such as genetic disorders or novel pathogen resistance. the increasing availability of genome sequences through the application of next-generation massive sequencing technologies has allowed the study of genomic polymorphisms at both the interspecific and intraspecific levels, thus helping to understand how species adapt to ...	2013	24219589
development of ssr markers by next-generation sequencing of korean landraces of chamoe (cucumis melo var. makuwa).	the oriental melon (cucumis melo var. makuwa), called 'chamoe' in korean, is a popular fruit crop cultivated mainly in asia and a high-market value crop in korea. to provide molecular breeding resources for chamoe, we developed and characterized genomic ssr markers from the preliminary illumina read assemblies of gotgam chamoe (one of the major landraces; km) and sw3 (the breeding parent). mononucleotide motifs were the most abundant type of markers, followed by di-, tri-, tetra-, and pentanucle ...	2013	24096890
non-destructive assessment of aroma volatiles from a climacteric near-isogenic line of melon obtained by headspace stir-bar sorptive extraction.	a climacteric aromatic near-isogenic line (nil) of melon (cucumis melo l.) sc3-5-1 contained an introgression of the non-climacteric korean cultivar "shongwan charmi" accession pi 161375 (sc) in the genetic background of the non-climacteric cultivar "piel de sapo" (ps). the aroma production was monitored during ripening at 21 °c in intact fruit using headspace sorptive bar extraction (hsse). bars were composed of polydimethylsiloxane (pdms) and aromas were desorbed and analyzed by gas-chromatogr ...	2013	28239125
genetic and chemical characterization of an ems induced mutation in cucumis melo crtiso gene.	in order to broaden the available genetic variation of melon, we developed an ethyl methanesulfonate mutation library in an orange-flesh 'charentais' type melon line that accumulates β-carotene. one mutagenized m2 family segregated for a novel recessive trait, a yellow-orange fruit flesh ('yofi'). hplc analysis revealed that 'yofi' accumulates pro-lycopene (tetra-cis-lycopene) as its major fruit pigment. the altered carotenoid composition of 'yofi' is associated with a significant change of the ...	2013	23973661
synthesis of 9-oxononanoic acid, a precursor for biopolymers.	polymers based on renewable resources have become increasingly important. the natural functionalization of fats and oils enables an easy access to interesting monomeric building blocks, which in turn transform the derivative biopolymers into high-performance materials. unfortunately, interesting building blocks of medium-chain length are difficult to obtain by traditional chemical means. herein, a biotechnological pathway is established that could provide an environmentally suitable and sustaina ...	2013	23934656
induction of antiviral responses by acibenzolar-s-methyl against cucurbit chlorotic yellows virus in melon.	cucurbit chlorotic yellows virus (ccyv) (family closteroviridae, genus crinivirus) is an emerging virus which causes severe diseases on melon (cucumis melo) plants. ccyv-infected melon plants display yellowing, mottling, chlorosis, or chlorotic spots on leaves. to develop a new control strategy, the potential for 1,2,3-benzothiadiazole-7-thiocarboxylic acid-s-methyl-ester (asm) to suppress ccyv infection was evaluated. asm treatment on melon plants greatly increased the expression levels of path ...	2013	23927428
evaluation of an analytical methodology using quechers and gc-sq/ms for the investigation of the level of pesticide residues in brazilian melons.	a multiresidue method based on the sample preparation by modified quechers and detection by gas chromatography coupled to single quadruple mass spectrometers (gc-sq/ms) was used for the analysis of 35 multiclass pesticides in melons (cucumis melo inodorus) produced in ceara-brazil. the rates of recovery for pesticides studied were satisfactory (except for the etridiazole), ranging from 85% to 117% with a relative standard deviation (rsd) of less than 15%, at concentrations between 0.05 and 0.20 ...	2013	23871010
the suitability of three galia melon cultivars and different types of cuts for the fresh-cut industry.	melons (cucumis melo l.) are widely cultivated in mediterranean countries. for fresh-cut melon elaboration, factors such as type of cultivar and cut have a significant impact on final product quality. the quality parameters from long, medium and short shelf-life galia melon cultivars, cut into different shapes such as slices, trapezoids and cylinders and stored up to 10 days at 5 °c, were evaluated in order to learn the suitability of these cultivars and type of cut for minimal processing.	2013	23860939
diversification and genetic differentiation of cultivated melon inferred from sequence polymorphism in the chloroplast genome.	molecular analysis encouraged discovery of genetic diversity and relationships of cultivated melon (cucumis melo l.). we sequenced nine inter- and intra-genic regions of the chloroplast genome, about 5500 bp, using 60 melon accessions and six reference accessions of wild species of cucumis to show intra-specific variation of the chloroplast genome. sequence polymorphisms were detected among melon accessions and other cucumis species, indicating intra-specific diversification of the chloroplast g ...	2013	23853513
assessment of a 1h high-resolution magic angle spinning nmr spectroscopy procedure for free sugars quantification in intact plant tissue.	in most plants, sucrose is the primary product of photosynthesis, the transport form of assimilated carbon, and also one of the main factors determining sweetness in fresh fruits. traditional methods for sugar quantification (mainly sucrose, glucose and fructose) require obtaining crude plant extracts, which sometimes involve substantial sample manipulation, making the process time-consuming and increasing the risk of sample degradation. here, we describe and validate a fast method to determine ...	2013	23824526
comparison of different methods for separation of haploid embryo induced through irradiated pollen and their economic analysis in melon (cucumis melo var. inodorus).	irradiated pollen technique is the most successful haploidization technique within cucurbitaceae. after harvesting of fruits pollinated with irradiated pollen, classical method called as "inspecting the seeds one by one" is used to find haploid embryos in the seeds. in this study, different methods were used to extract the embryos more easily, quickly, economically, and effectively. "inspecting the seeds one by one" was used as control treatment. other four methods tested were "sowing seeds dire ...	2013	23818825
[identification of two fusarium isolates and their crude toxin allelopathic effect on cucumis melo seedlings].	eight fungi isolates were obtained from fusarium-infected cucumis melo (melon) plants and their rhizosphere soils. taking melon cultivar 'xitian 1' as test material, the re-inoculation and seed germination experiments were conducted to investigate the pathogenicity and growth inhibition effect of these fungi isolates on melon. through the determination of the induced enzyme activities, resistant substance contents, and cell membrane permeability of potted melon roots, the allelopathic effect of ...	2013	23718002
flavour profiles of three novel acidic varieties of muskmelon (cucumis melo l.).	novel acidic varieties of muskmelon (cucumis melo l.) are emerging onto the uk market. these melons contain almost twice the amount of citric acid compared to standard melons and are described as 'zesty and fresh'. this study compared the flavour components of three acidic varieties with a standard galia-type melon. the volatile and semivolatile compounds were extracted, using dynamic headspace extraction (dhe) or solid-phase microextraction (spme) and solid phase extraction (spe), respectively, ...	2013	23561221
an integrated approach for flavour quality evaluation in muskmelon (cucumis melo l. reticulatus group) during ripening.	numerous and diverse physiological changes occur during fruit ripening and maturity at harvest is one of the key factors influencing the flavour quality of fruits. the effect of ripening on chemical composition, physical parameters and sensory perception of three muskmelon (cucumis melo l. reticulatus group) cultivars was evaluated. significant correlations emerging from this extensive data set are discussed in the context of identifying potential targets for melon sensory quality improvement. a ...	2013	23561094
snp genotyping in melons: genetic variation, population structure, and linkage disequilibrium.	novel sequencing technologies were recently used to generate sequences from multiple melon (cucumis melo l.) genotypes, enabling the in silico identification of large single nucleotide polymorphism (snp) collections. in order to optimize the use of these markers, snp validation and large-scale genotyping are necessary. in this paper, we present the first validated design for a genotyping array with 768 snps that are evenly distributed throughout the melon genome. this customized illumina goldeng ...	2013	23381808
clinical efficacy of a novel topical formulation for vitiligo: compared evaluation of different treatment modalities in 149 patients.	current vitiligo treatments are not always satisfactory for both patients and dermatologists. recently, combination therapies have been introduced in order to obtain better results and reduce risks in the management of the disease. novel efficacious products are needed to improve the therapeutic possibilities of dermatologists in the respect of safety for the patients. the objective of the present study was to evaluate the effects of a novel topical in a gel formulation containing phenylalanine, ...	2013	23046028
genetic and virulence variability among erwinia tracheiphila strains recovered from different cucurbit hosts.	the causal agent of cucurbit bacterial wilt, erwinia tracheiphila, has a wide host range in the family cucurbitaceae, including economically important crops such as muskmelon (cucumis melo), cucumber (c. sativus), and squash (cucurbita spp.). genetic variability of 69 e. tracheiphila strains was investigated by repetitive-element polymerase chain reaction (rep-pcr) using boxa1r and eric1-2 primers. fingerprint profiles revealed significant variability associated with crop host; strains isolated ...	2013	23927426
molecular cloning, characterization, and expression of cuc m 2, a major allergen in cucumis melo.	several studies reported the clinical features of ige-mediated hypersensitivity after ingestion of melon. melon allergy is a common ige-mediated fruit allergy in iran. this prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the ige-binding capacity of the natural protein with the recombinant allergen, and to determine cross-reactivity of the major allergen with closely-related allergens from other plants displaying clinical cross-r ...	2013	26989709
purification, cdna cloning and recombinant protein expression of a phloem lectin-like anti-insect defense protein bplp from the phloem exudate of the wax gourd, benincasa hispida.	latex and other exudates in plants contain various proteins that are thought to play important defensive roles against herbivorous insects and pathogens. herein, the defensive effects of phloem exudates against the eri silkworm, samia ricini (saturniidae, lepidoptera) in several cucurbitaceous plants were investigated. it was found that phloem exudates are responsible for the defensive activities of cucurbitaceous plants, such as the wax gourd benincasa hispida and cucumis melo, especially in b. ...	2013	23453909
catabolism of l-methionine in the formation of sulfur and other volatiles in melon (cucumis melo l.) fruit.	sulfur-containing aroma volatiles are important contributors to the distinctive aroma of melon and other fruits. melon cultivars and accessions differ in the content of sulfur-containing and other volatiles. l-methionine has been postulated to serve as a precursor of these volatiles. incubation of melon fruit cubes with ¹³c- and ²h-labeled l-methionine revealed two distinct catabolic routes into volatiles. one route apparently involves the action of an l-methionine aminotransferase and preserves ...	2013	23402686