Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| serological survey of rhodococcus equi infection in horses in hokkaido. | serological survey of rhodococcus equi infection in horses in hokkaido was performed using elisa. of 2,879 horse sera, 318 (11.0%) gave antibody-positive (od greater than or equal to 0.3) reactions. the antibody-positive rate of female was significantly higher (p less than 0.01) than that of male, and no statistical difference between anglo-arab and thoroughbred was detected in the antibody-positive rate. the maximum antibody-positive rate (27.1%) was shown at 14 years of age. the antibody-posit ... | 1992 | 1391174 |
| distribution and antimicrobial susceptibility of rhodococcus equi from clinical specimens. | rhodococcus equi, an unusual gram positive aerobic actinomycete, was first described as a respiratory pathogen of livestock in 1923. reports of human clinical illness have emphasized r. equi as a cause of invasive pulmonary infection in severely immunocompromised patients and, recently, have implicated it as a cause of pneumonia, bacteremia and disseminated infection in hiv-infected patients. to determine the distribution of r. equi we evaluated 107 isolates referred to the centers for disease c ... | 1992 | 1397208 |
| nitrilase from rhodococcus rhodochrous j1. sequencing and overexpression of the gene and identification of an essential cysteine residue. | the amino acid sequences of the nh2 terminus and internal peptide fragments of a rhodococcus rhodochrous j1 nitrilase were determined to prepare synthetic oligonucleotides as primers for the polymerase chain reaction. a 750-base dna fragment thus amplified was used as the probe to clone a 5.4-kilobase psti fragment coding for the whole nitrilase. the nitrilase gene modified in the sequence upstream from the presumed atg start codon was expressed to approximately 50% of the total soluble protein ... | 1992 | 1400390 |
| the plasmid-encoded chloramphenicol-resistance protein of rhodococcus fascians is homologous to the transmembrane tetracycline efflux proteins. | the nucleotide sequence of the chloramphenicol-resistance gene (cmr) of rhodococcus fascians ncppb 1675 (located on the conjugative plasmid prf2) allowed the identification of two possible open reading frames (orfs), of which orf1 was consistent with the mutational analysis. biochemical analysis of cmr revealed that it does not encode an antibiotic-modifying enzyme. the amino acid sequence of orf1 predicted a hydrophobic protein, with 12 putative membrane-spanning domains, homologous to proteins ... | 1992 | 1406276 |
| therapy of rhodococcus equi disseminated infections in nude mice. | rhodococcus equi is a facultative, intracellular, gram-positive coccobacillus increasingly reported as an opportunistic pathogen in human immunodeficiency virus-positive patients. however, the optimal drug regimen for treating r. equi pulmonary or systemic infections is not yet known. therefore, a model of intravenously infected nude mice with disseminated infection was created to study the efficacy of antibiotics alone or in combination as determined by the reduction of bacterial cfu per gram i ... | 1992 | 1416823 |
| central venous catheter sepsis caused by unusual gordona (rhodococcus species: identification with a digoxigenin-labeled rdna probe. | we describe central line sepsis caused by gordona (rhodococcus) species in two patients, which complicated receipt of long-term total parenteral nutrition at home. species identification was attempted by conventional biochemical analysis and analysis of polymorphisms in the ribosomal rna genes with use of a digoxigenin-labeled rdna probe. using these techniques, we identified our first patient's isolate as gordona terrae. the isolate from our second patient was biochemically atypical and could n ... | 1992 | 1420683 |
| good response to antibiotic treatment of lung infection due to rhodococcus equi in a patient infected with human immunodeficiency virus. | 1992 | 1420703 | |
| successful medical therapy of rhodococcus equi pneumonia in a patient with hiv infection. | a 34-year-old hiv-infected man was successfully treated with antimicrobial therapy alone for rhodococcus equi pneumonia and has survived longer than six months. in the current literature, only two of seven hiv-infected patients so treated have survived as long as six months. based on our experience and the available literature, it seems reasonable to treat hiv-infected patients with r equi pneumonia who do not require surgical intervention with prolonged intravenous therapy followed by long-term ... | 1992 | 1424910 |
| the biology and genetics of the genus rhodococcus. | the genus rhodococcus is a unique taxon consisting of microorganisms that exhibit broad metabolic diversity, particularly to hydrophobic compounds such as hydrocarbons, chlorinated phenolics, steroids, lignin, coal, and petroleum. advances in chemical, numerical, and molecular systematic methods have contributed greatly to the circumspection of the rhodococci, including the development of diagnostic fluoregenic probes for improved biochemical profiling and identification. bioprocessing systems e ... | 1992 | 1444254 |
| degradation of 2,4-dinitrophenol by two rhodococcus erythropolis strains, hl 24-1 and hl 24-2. | two rhodococcus erythropolis strains, hl 24-1 and hl 24-2, were isolated from soil and river water by their abilities to utilize 2,4-dinitrophenol (0.5 mm) as the sole source of nitrogen. although succinate was supplied as a carbon and energy source during selection, both isolates could utilize 2,4-dinitrophenol also as the sole source of carbon. both strains metabolized 2,4-dinitrophenol under concomitant liberation of stoichiometric amounts of nitrite and 4,6-dinitrohexanoate as a minor dead-e ... | 1992 | 1444407 |
| initial hydrogenation during catabolism of picric acid by rhodococcus erythropolis hl 24-2. | rhodococcus erythropolis hl 24-2, which was originally isolated as a 2,4-dinitrophenol-degrading bacterium, could also utilize picric acid as a nitrogen source after spontaneous mutation. during growth, the mutant hl pm-1 transiently accumulated an orange-red metabolite, which was identified as a hydride-meisenheimer complex of picric acid. this complex was formed as the initial metabolite and further converted with concomitant liberation of nitrite. 2,4,6-trinitrocyclohexanone was identified as ... | 1992 | 1444408 |
| isopropylbenzene (cumene)--a new substrate for the isolation of trichloroethene-degrading bacteria. | various bacterial isolates from enrichments with isopropylbenzene (cumene), toluene or phenol as carbon and energy sources were tested as to their potential to oxidize trichloroethene (tce). in contrast to toluene and phenol, all isolates enriched on isopropylbenzene were able to oxidize tce. two isolates, strain jr1 and strain bd1, were identified as pseudomonas spec. and as rhodococcus erythropolis, respectively. tce oxidation was accompanied by the liberation of stoichiometric amounts of chlo ... | 1992 | 1444717 |
| sequence of a rhodococcus gene encoding a protein with extensive homology to the mammalian propionyl-coa carboxylase beta chain. | sequence analysis of a 2-kilobase dna fragment from rhodococcus sp. ni86/21 revealed an open reading frame encoding a 476-amino-acid protein with striking homology to the rat and human propionyl-coa carboxylase beta subunits. the nucleotide sequence of a corresponding prokaryotic gene has not yet been reported. upstream, the c-terminal part of a putative beta-ketoacyl synthase was identified. | 1992 | 1452030 |
| rhodococcus equi cavitary pneumonia in hiv-infected patients: an unsuspected opportunistic pathogen. | two patients seropositive for human immunodeficiency virus (hiv) and with no previous acquired immunodeficiency syndrome-defining conditions developed cavitary pneumonia and pleural disease caused by rhodococcus equi. r. equi was isolated from these patients' sputum and lung biopsy specimens, respectively, but the microorganism was initially considered to be a contaminant (patient 1) or misidentified as a nontuberculous mycobacterium (patient 2). the r. equi infection was fatal in one patient, w ... | 1992 | 1453322 |
| overexpression of a rhodococcus erythropolis protein in escherichia coli with immunological identity to the rhodococcus steroid 1-dehydrogenase. immunoelectron microscopic localization and electrophoretic studies. | the recombinant escherichia coli k-12 strain chi 6060 harbouring the plasmid pya 1201 with a gene from rhodococcus erythropolis imet 7030 overexpressed a protein which reacts with a monospecific antiserum against the steroid 1-dehydrogenase (sdh) from the same rhodococcus strain. it was shown previously that this recombinant protein exhibits no enzymatic activity. by immunogold labelling the protein was localized on ultrathin sections of the recombinant e. coli strain. after cultivation at 37 de ... | 1992 | 1460569 |
| [the first isolation of dermatophilus congolensis van saceghem 1913 in czechoslovakia]. | the authors present a report on the first detection of the strain dermatophilus congolensis in czechoslovakia. the strain was isolated from ulcerations on the dorsal part of the foot of a 47-year-old patient who previously worked for several years in libya. based on examinations of smears from cultures of an isolated strain and four strains from a collection, from experimental dermatofilosis of a rabbit the authors describe the micromorphology of strains of d. congolensis under a light and elect ... | 1992 | 1464078 |
| influence of inoculation route on virulence of rhodococcus equi in mice. | virulence of r. equi atcc 33701 was compared by the intraperitoneal (i.p.) and intravenous (i.v.) routes in mice. strain atcc 33701 was more virulent by the i.v. than the i.p. route. the ld50 of strain atcc 33701 by either route correlated with the initial number of bacteria in the liver and spleen at day 0. | 1992 | 1474937 |
| molecular basis for biosynthesis and accumulation of polyhydroxyalkanoic acids in bacteria. | the current knowledge on the structure and on the organization of polyhydroxyalkanoic acid (pha)-biosynthetic genes from a wide range of different bacteria, which rely on different pathways for biosynthesis of this storage polyesters, is provided. molecular data will be shown for genes of alcaligenes eutrophus, purple non-sulfur bacteria, such as rhodospirillum rubrum, purple sulfur bacteria, such as chromatium vinosum, pseudomonads belonging to rrna homology group i, such as pseudomonas aerugin ... | 1992 | 1476773 |
| molecular weight determination of methyl esters of mycolic acids using thermospray mass spectrometry. | methyl esters of normal fatty acids, corynomycolate and corynomycolenate were used as model compounds for thermospray mass spectrometric procedures for molecular weight determination of the related nocardial mycolic acids. by using ammonium acetate at the positive ion generator, in both cases, a family of ions was produced. the following members were found and corresponded to the adducts: (1) m + h; m + nh4 and m + h + nh4 for methyl esters of normal fatty acids, whereas m + h, m + 2h and m + h ... | 1992 | 1486659 |
| structural studies of an emulsion-stabilizing exopolysaccharide produced by an adhesive, hydrophobic rhodococcus strain. | the primary structure of an emulsion-stabilizing exopolysaccharide from the adhesive, hydrophobic rhodococcus strain no. 33 was elucidated by nmr spectroscopy, methylation analyses, periodate oxidation and oligosaccharide analyses. the polysaccharide ps-33 consisted of rhamnose, galactose, glucose and glucuronic acid in molar ratios of 2:1:1:1. the main chain contained 3-substituted alpha-d-glucuronic acid linked to the 3-position at alpha-l-rhamnose, in addition to 3-substituted residues of bet ... | 1992 | 1487724 |
| cloning of dna from a rhodococcus strain conferring the ability to decolorize sulfonated azo dyes. | azo dyes are recalcitrant pollutants. two sulfonated azo dyes, orange ii and amido black, are effectively decolorized by certain nocardioform strains of the genus rhodococcus. a mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone dna conferring this ability, by screening a bcli library constructed from dna of a decolorizing strain. the relevant genetic information was located on a 6.3-kb fragment of dna. | 1992 | 1490602 |
| [rhodococcus equi infections in aids: personal cases]. | we report on the occurrence of an uncommon pathogen, rhodococcus equi (r.e.) in hiv infection. in the period february 1990-july 1991 we performed the diagnosis of r.e. infection (lung, lung-central nervous system) in two infected patients, aged 27 and 31 years, both males, drug addicts, classified as cdc iv. in the first of them an cavitating right bronchopneumonia (bpm) was diagnosed as of tuberculous origin, on the ground of the microscopic demonstration of an acid-fast, gram-positive strain i ... | 1992 | 1491893 |
| septicaemia due to a new species of rhodococcus that contaminated closed system packed red blood cells--cure with imipenem monotherapy. | 1992 | 1493992 | |
| disseminated rhodococcus equi infection initially presenting as foot mycetoma in an hiv-positive patient. | 1992 | 1503695 | |
| effect of cosubstrates on the dechlorination of selected chlorophenolic compounds by rhodococcus erythropolis 1cp. | effects of aromatic and nonaromatic cosubstrates on the rate of 2,4-dichlorophenol degradation by r. erythropolis 1cp were studied under growth and nongrowth conditions. glucose and maltose were found to accelerate 2,4-dichlorophenol (initial conc. 50 mg/l) dechlorination from 11 days to 24 and 20 h, respectively. the stimulating effect was observed within the range of glucose concentration of 0.5-2.0 g/l in the medium. ascorbic acid at a concentration of 10-70 mg/l as a nongrowth substrate also ... | 1992 | 1512708 |
| [multifactor study of combined effects of an antibiotic and polysaccharide in experimental infection]. | multifactorial analysis was applied to the study of the combined effect of doxycycline and a polysaccharide of microbial origin in experimental plague infection. a marked synergistic action of the antibiotic and polysaccharide used in subtherapeutic doses in treatment of the infection was observed. by the results of the experiments polynomial statistic models of the 2nd order were designed and nomographs or equal level lines were plotted. the models and nomographs described the animal survival r ... | 1992 | 1514853 |
| [rhodococcus equi pneumonia: 1st opportunistic manifestation in a patient infected with hiv-1]. | 1992 | 1524356 | |
| pulmonary abscess due to a rifampin and fluoroquinolone resistant rhodococcus equi strain in a hiv infected patient. | 1992 | 1526243 | |
| identification, cloning and sequence analysis of the poly(3-hydroxyalkanoic acid) synthase gene of the gram-positive bacterium rhodococcus ruber. | the first polyhydroxyalkanoic acid (pha) synthase gene (phbcrr) of a gram-positive bacterium was cloned from a genomic library of rhodococcus ruber in the broad-host-range plasmid vector prk404. the hybrid plasmid harboring phbcrr allowed the expression of polyhydroxybutyric acid (phb) synthase activity and restored the ability of phb synthesis in a phb-negative mutant of alcaligenes eutrophus. nucleotide sequence analysis of phbcrr revealed an open reading frame of 1686 bp starting with the rar ... | 1992 | 1526467 |
| the n-terminal amino acid sequences of brevibacterium sp. r312 nitrile hydratase. | nitrile hydratase from brevibacterium sp. r312 was purified to homogeneity. the isoelectric point was 5.75. the two kinds of subunits were separated by reverse phase hplc and their n-terminal amino acid sequences were found to be identical to those of rhodococcus sp. n-774 nitrile hydratase. | 1992 | 1527703 |
| utilization of 1,3,5-trihydroxybenzene (phloroglucinol) by a soil isolate, rhodococcus species bpg-8. | a gram-positive bacterial strain was isolated from oil rich soil in newfoundland and found to utilize various di- and trihydroxylated aromatic compounds as a source of carbon and energy. this bacterium exhibited rod/coccus dimorphism during its growth cycle. chemical analysis of cell wall composition (amino acids, sugars, and fatty acids) was performed using gas chromatography-mass spectrophotometry and high pressure liquid chromatography. comparison of both acid production and growth substrates ... | 1992 | 1527706 |
| localization of the steroid 1-dehydrogenase in rhodococcus erythropolis imet 7030 by immunoelectron microscopy. | the steroid 1-dehydrogenase of rhodococcus erythropolis imet 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. the labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. a consid ... | 1992 | 1527710 |
| analysis of the immunodominant antigens of corynebacterium pseudotuberculosis. | antibodies to seven antigens in a whole cell lysate of corynebacterium pseudotuberculosis ranging in molecular mass from 22 to 120 kilodaltons (kda) were present in sera of 40 sheep and goats infected with c. pseudotuberculosis. three antigens of about 120, 68, and 31.5 kda in size were consistently detected with sera from all animals and twenty-two sera had antibodies to 64, 43, 40, and 22 kda antigens. none of these antigens were detected by sera from 160 sheep in a c. pseudotuberculosis-free ... | 1992 | 1531563 |
| [lung abscess caused by rhodococcus (corynebacterium) equi in hiv infection. two cases]. | rhodococcus (corynebacterium) equi is a well-known gram positive bacillus which is usually pathogenic in farm animals but rarely causes diseases in humans. only 30 cases of human infection have been reported in the literature. r. equi has recently been added to the list of opportunistic agents in severe hiv infection. its most common manifestation in immunocompromised is a slowly progressive pneumonia which may cavitate. r. equi pulmonary infections are difficult to treat: they usually require p ... | 1992 | 1533035 |
| in vitro activity of teicoplanin and vancomycin against gram-positive bacteria from human clinical and veterinary sources. | the minimum inhibitory concentration (mic) of teicoplanin and vancomycin was determined by the agar dilution method for 186 gram-positive bacteria from human clinical and veterinary sources. teicoplanin mic values were less than or equal to 4 micrograms/ml for 94% of staphylococci (group a, n = 52) and less than or equal to 2 micrograms/ml for all streptococci, enterococci, aerococci and pediococci (group b, n = 75). seventy-eight percent of gram-positive rods, rhodococcus and leuconostoc spp. ( ... | 1992 | 1535201 |
| rhodococcus equi endobronchial mass with lung abscess in a patient with aids. | an endobronchial lesion with lung abscess in a patient with aids was due to rhodococcus equi. the patient responded to triple chemotherapy. | 1992 | 1539150 |
| transformation of an insect symbiont and expression of a foreign gene in the chagas' disease vector rhodnius prolixus. | a shuttle plasmid was developed that is capable of replicating both in escherichia coli and in rhodococcus rhodnii, a bacterial symbiont of the chagas' disease vector rhodnius prolixus. we have been able to transform r. rhodnii with this plasmid, infect aposymbiotic r. prolixus with the transformed symbionts, select with the antibiotic thiostrepton, and re-isolate genetically altered symbionts from the insects following successive molts. symbiotic bacteria are potentially valuable as vehicles fo ... | 1992 | 1539755 |
| fasciation induction by the phytopathogen rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. | rhodococcus fascians is a nocardiform bacteria that induces leafy galls (fasciation) on dicotyledonous and several monocotyledonous plants. the wild-type strain d188 contained a conjugative, 200 kb linear extrachromosomal element, pfid188. linear plasmid-cured strains were avirulent and reintroduction of this linear element restored virulence. pulsed field electrophoresis indicated that the chromosome might also be a linear molecule of 4 megabases. three loci involved in phytopathogenicity have ... | 1992 | 1547783 |
| recurrent pneumonia due to rifampicin-resistant rhodococcus equi in a patient infected with hiv. | 1992 | 1548410 | |
| construction of an insertional-inactivation cloning vector for escherichia coli using a rhodococcus gene for indigo production. | pslh8, an insertional-inactivation cloning vector for escherichia coli has been constructed by inserting a pigment gene (probably encoding an indole dioxygenase) from rhodococcus sp. atcc 21145 into puc18. wild-type e. coli colonies containing pslh8 produce insoluble indigo and turn dark blue on unsupplemented lb agar. insertion of dna fragments into the unique bamhi, ecori, ecorv, hindiii, psti, sphi and ssti polylinker cloning sites disrupts the reading frame of the fully sequenced pigment gen ... | 1992 | 1556550 |
| localization of the cholesterol oxidase in rhodococcus erythropolis imet 7185 studied by immunoelectron microscopy. | rhodococcus erythropolis imet 7185 produces an inducible cholesterol oxidase (cod) which can easily be extracted by treatment of cells with 0.1% triton x-100. the yield of the enzyme was 3.3 u/g wet wt from induced cells, which is about 5 times more than from non-induced cells. a study of the location of cod on intact cells and on ultrathin sections by means of immunogold electron microscopy revealed the following distribution, which corresponds with the biochemical results: (1) cod, which is ex ... | 1992 | 1567784 |
| light-induced oxidation of iron atoms in a photosensitive nitrile hydratase. | the photoactivation process of a photosensitive nitrile hydratase (nhase) from rhodococcus sp. n-771 has been investigated by 57fe mössbauer spectroscopy and magnetic susceptibility measurements in order to clarify the behavior of iron atoms in the enzyme. mössbauer spectra of inactive nhase gave two symmetric-doublet components indicating the presence of two iron species, while that of the active nhase gave a single symmetric doublet indicating the presence of a single iron species. magnetic su ... | 1992 | 1568476 |
| rhodococcus equi infection in patients with aids. | rhodococcus equi is an emerging opportunistic pathogen of hiv-i infected patients. it is an aerobic, gram-positive coryneform bacterium which acts as a facultative intracellular micro-organism, multiplying in the phagosome of macrophages. eighteen cases of r. equi infection in hiv-i positive patients have now been reported. sixteen of these had pneumonia, of which 12 had cavitating lung lesions. a history of contact with farm animals, which are the primary hosts of r. equi, was found in only thr ... | 1992 | 1569304 |
| isolation and characterization of cesium-accumulating bacteria. | cesium-accumulating bacteria, strains cs98 and cs402, were isolated from soil by a radioactive autoradiographic method using 137cs. these strains displayed the rod-coccus growth cycle and contained mesodiaminopimelic acid, mycolic acids, and tuberculostearic acids. the major menaquinone of cs98 was mk-8(h2). on the basis of these characteristics, strain cs98 was identified as rhodococcus erythropolis and strain cs402 was classified in the genus rhodococcus. the maximum values of cesium removal e ... | 1992 | 1575473 |
| [the sulfate-reducing capacity of bacteria in the genus pseudomonas]. | active sulphate-reducing microorganism which belongs to the genus pseudomonas has been distinguished and described. the culture is a facultative aerobe, optimum eh is -170-180 mv. pseudomonas sp. being cultivated under strictly anaerobic conditions sulphate-reduction proceeds more intensively than under aerobic conditions. this fact should be taken into account under treatment of industrial sewage. | 1992 | 1584088 |
| case report: rhodococcus equi pneumonia in a patient infected by the human immunodeficiency virus. | rhodococcus equi, a facultative intracellular bacterium, is a common cause of pulmonary infection in farm animals, especially foals. pulmonary and disseminated infection caused by this organism is occasionally seen in humans, especially in patients whose cell-mediated immunity has been altered by glucocorticoids or cytotoxic chemotherapy. not surprisingly, the organism may cause serious disease in human immunodeficiency virus (hiv)-infected humans whose t cell-dependent immune system has been pr ... | 1992 | 1595781 |
| nad-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from amycolatopsis methanolica. | nad-linked, factor-dependent formaldehyde dehydrogenase (fd-fa1dh) of the gram-positive methylotrophic bacterium, amycolatopsis methanolica, was purified to homogeneity. it is a trimeric enzyme with identical subunits (molecular mass 40 kda) containing 6 atoms zn/enzyme molecule. the factor is a heat-stable, low-molecular-mass compound, which showed retention on an aminex hpx-87h column. inactivation of the factor occurred during manipulation, but activity could be restored by incubation with di ... | 1992 | 1597190 |
| [rhodococcus equi in hiv infected patients: 2 new cases]. | we reported two hiv infected patients with bacteremia and pneumonia due to rhodococcus equi. none of them had suffer any opportunistic infection before this episode. clinical presentation includes respiratory tract symptoms of subacute onset and fever. the x-ray examination in both cases revealed pneumonia and lung abscess in upper lobes as well as lung infiltrates in other lobes. the microorganism was isolated in lung fine needle aspiration, bronchoalveolar lavage and blood cultures in both cas ... | 1992 | 1606224 |
| in-vitro antimicrobial susceptibility of rhodococcus equi. | rhodococcus equi is an intracellular facultative, gram-positive cocco-bacillary organism of increasing importance as a pulmonary pathogen in hiv-positive patients. this study was carried out to evaluate the optimal antibiotic combinations for treating such infections. four human r. equi isolates and one reference strain were tested for their susceptibilities to 36 antibiotics. in-vitro the most active antibiotics were amikacin, gentamicin, netilmicin, erythromycin, clarithromycin, roxithromycin, ... | 1992 | 1607327 |
| polyacrylamide gel electrophoresis analysis of ribosomal protein: a new approach for actinomycete taxonomy. | the ribosomal (r)-proteins from eleven streptomyces strains representing various numerical taxonomic clusters were compared by two-dimensional polyacrylamide-gel electrophoresis (2d-page). the protein patterns were specific for each species. an attempt was made to identify one strain of streptomyces by both traditional taxonomic methods and 2d-page analysis of the r-protein patterns. both methods identified the strain as streptomyces lavendulae, and protein pattern analysis also showed that s. g ... | 1992 | 1612446 |
| virulence-associated 15- to 17-kilodalton antigens in rhodococcus equi: temperature-dependent expression and location of the antigens. | virulent rhodococcus equi showing 15- to 17-kda antigens, which is virulent in mice, was found to harbor an 85-kbp plasmid, and the 15- to 17-kda antigens were found to be associated with possession of the 85-kbp plasmid of r. equi (s. takai, t. sekizaki, t. ozawa, t. sugawara, y. watanabe, and s. tsubaki, infect. immun. 59:4056-4060, 1991). the expression of these antigens was temperature regulated: when cells were grown at a low temperature (25 to 32 degrees c), they did not express them, wher ... | 1992 | 1612765 |
| hemolytic interactions of dermatophilus congolensis. | the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ... | 1992 | 1621476 |
| naphthalene degradation via salicylate and gentisate by rhodococcus sp. strain b4. | rhodococcus sp. strain b4, isolated from a soil sample contaminated with polycyclic aromatic hydrocarbons, grows with naphthalene as the sole source of carbon and energy. salicylate and gentisate were identified as intermediates in the catabolism of naphthalene. in contrast to the well-studied catabolic pathway encoded by the nah7 plasmid of pseudomonas putida, salicylate does not induce the genes of the naphthalene-degradative pathway in rhodococcus sp. strain b4. the key enzymes of naphthalene ... | 1992 | 1622263 |
| high-performance liquid chromatography of corynomycolic acids as a tool in identification of corynebacterium species and related organisms. | a high-performance liquid chromatography (hplc) study of 307 strains of corynebacterium species and related taxa revealed that strains classified as "corynebacterium aquaticum"; "corynebacterium asperum"; and centers for disease control (cdc) groups 1, 2, a-3, a-4, a-5, b-1, b-3, e, f-2, and i-2 as well as some unidentified coryneforms do not contain any corynomycolic acids; therefore, they should not be included in the genus corynebacterium. such an hplc method of identification permitted the c ... | 1992 | 1624556 |
| a colorimetric assay for measuring cell-free and cell-bound cholesterol oxidase. | cholesterol oxidase (cholesterol:oxygen oxidoreductase, ec 1.1.3.6) catalyzes the conversion of sterol delta 5-3 beta-alcohol to the corresponding delta 4-3-ketone with the reduction of oxygen to hydrogen peroxide. rhodococcus species gk 1, a soil isolated microbe, produces an extracellular and a membrane-bound cholesterol oxidase; the latter is bound to the outer surface of the microbial cell membrane. a simple and sensitive assay is described to measure the two enzyme types; no enzyme extracti ... | 1992 | 1630278 |
| an assay to quantitate the binding of rhodococcus equi to macrophages. | a rhodococcus equi radiobinding assay has been developed using organisms labeled with 3h-uracil. these labeled organisms resemble their unlabeled counterparts with respect to colony morphology, viability, and buoyant density. bacteria routinely incorporate between 5 x 10(-3) and 5 x 10(-2) counts per minute per colony forming unit (cfu) which in this assay allows the detection of fewer than 0.2 cfu per macrophage. once incorporated, greater than 90% of the label remains bacterial associated for ... | 1992 | 1632069 |
| [pulmonary infection caused by rhodococcus equi in a renal transplant recipient]. | rhodococcus equi is a primarily pathogen in animals and it has only rarely been seen in immunocompromised humans. we describe our experience with an hiv-negative patient with a functioning renal graft under cyclosporin-azathioprine-prednisone therapy. the patient died after a two months recurrent multifocal pneumonia. the cultures from sputum and blood were negatives but r. equi was isolated from lungs in the necropsy. most of the 41 cases we had found in the literature had aids or hiv infection ... | 1992 | 1635409 |
| immunohistochemical observations on pneumonic lesions caused by rhodococcus equi in foals. | an immunohistochemical analysis of rhodococcus equi-induced pneumonia in 10 foals was performed by biotin-streptavidin system. the detection of r. equi was more sensitive in immuno-stain using anti-r. equi serum than in gram's stain. this bacteria also reacted to anti-bcg serum. lysozyme and alpha 1-antitrypsin were detectable in macrophages. a particularly intense staining was observed in association with intracellular bacteria. though a degree of reaction for alpha 1-antichymotrypsin was very ... | 1992 | 1643174 |
| development of a host-vector system in a rhodococcus strain and its use for expression of the cloned nitrile hydratase gene cluster. | two different types of plasmid were isolated from strains of rhodococcus rhodochrous. two plasmids, of the same type but from different strains, were combined with escherichia coli plasmids carrying antibiotic resistance markers to develop e. coli-rhodococcus shuttle vectors. the ampicillin and kanamycin resistance markers served for selection in rhodococcus. electroporation was used to introduce recombinant plasmid dna into r. rhodochrous atcc 12674 at a frequency of 5 x 10(7) transformants per ... | 1992 | 1645124 |
| cloning, sequence analysis, and expression of the flavobacterium pentachlorophenol-4-monooxygenase gene in escherichia coli. | the pcpb gene of flavobacterium sp. strain atcc 39723 was cloned by using a degenerate primer designed from the n-terminal sequence of the purified enzyme. the nucleotide sequence of pcpb was determined and found to encode an open reading frame of 1,614 nucleotides, yielding a predicted translation product of 538 amino acids, in agreement with the estimated size of the purified protein analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the transcriptional start of pcpb was fo ... | 1993 | 7678243 |
| rhodococcus species fatal infection in an immunocompetent host. | a 24-year-old woman had fatal pneumonia-associated adult respiratory distress syndrome caused by rhodococcus species. histological examination of lung biopsy tissue showed intracellular coccobacillary microorganisms. antimicrobial susceptibility tests on the patient's blood isolate showed that it was resistant to clindamycin and norfloxacin but susceptible to several other antimicrobial agents. also, the isolate's biochemical reactions and pattern of rna gene-containing restriction fragments wer ... | 1993 | 7683869 |
| development of an enzymatic process for manufacturing acrylamide and recent progress. | 1993 | 7763351 | |
| characterization of nitrile hydratase genes cloned by dna screening from rhodococcus erythropolis. | southern hybridization analysis using the genes encoding the alpha- and beta-subunits of nitrile hydratase (nhase) from rhodococcus sp. n-774 as probe suggested that two r. erythropolis strains, jcm6823 and jcm2892, among 31 strains mainly from japan culture of microorganisms (jcm) have nhase genes. restriction analysis of dna fragments showing positive hybridization showed that each fragment carried a nucleotide sequence very similar to that of the nhase genes from rhodococcus sp. n-774. nucleo ... | 1993 | 7764017 |
| production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile by the combination of nitrile hydratase and stereoselective amidase in rhodococcus equi tg328. | a new soil isolate, tentatively identified as rhodococcus equi tg328, was found to be effective in the production of s-(+)-2-phenylpropionic acid from (r,s)-2-phenylpropionitrile. the conversion is catalysed by two enzymes. first, a nitrile hydratase converts the (r,s)-nitrile to (r,s)-2-phenylpropionamide. second, a stereoselective amidase converts the s-(+)-amide to s-(+)-2-phenylpropionic acid. conditions for optimal enzyme production and accumulation of s-(+)-2-phenylpropionic acid by restin ... | 1993 | 7764117 |
| amidase coupled with low-molecular-mass nitrile hydratase from rhodococcus rhodochrous j1. sequencing and expression of the gene and purification and characterization of the gene product. | the cloned 9.4-kb insert of plasmid pnhj20l containing low-molecular-mass nitrile hydratase (l-nhase) gene from rhodococcus rhodochrous j1 [kobayashi, m. et al. (1991) biochim. biophys. acta 1129, 23-33] was digested with various restriction enzymes, and the trimmed fragments were inserted into puc18 or puc19. a 1.96-kb ecori-sphi region located 1.9-kb downstream of the l-nhase gene was found to be essential for the expression of amidase activity in escherichia coli; the gene arrangement of the ... | 1993 | 7916690 |
| rhodococcus equi infection: a cause of cavitary pulmonary disease in immuno-compromised patients. | rhodococcus equi is a facultatively-aerobic, gram-positive and intracellular bacteria that causes pulmonary and disseminated infections in immuno-compromised patients. it is a common agent that causes pneumonia in farm animals and only rarely is transmitted to man. i report a case of pneumonia caused by this agent in a patient with aids who had been misdiagnosed as tuberculosis and treated as such for five months before the correct diagnosis was made. the diagnosis should be suspected on clinica ... | 1993 | 8055560 |
| lack of residual lung damage in horses in which rhodococcus equi-induced pneumonia had been diagnosed. | the effect of prior rhodococcus equi-induced pneumonia on pulmonary health was investigated in 5 horses (< 24 months old) using endoscopy, radiography, hematologic and bronchoalveolar lavage analyses, and pulmonary function testing. rhodococcus equi-induced pneumonia had been diagnosed in principal horses when they were foals. diagnosis was based on positive results of transtracheal aspiration and thoracic radiography at the time of initial clinical examination. results of reevaluation of the re ... | 1993 | 8116948 |
| endophthalmitis in auckland 1983-1991. | a review was made of 58 cases of suspected endophthalmitis presenting to auckland public hospital between january 1983 and july 1991 (103 months). thirty culture positive cases were found comprising 13 early onset postoperative, six delayed onset postoperative, five bleb-associated, four post-traumatic and two endogenous cases. the most frequent organism grown in postoperative endophthalmitis was staphylococcus epidermidis. no conclusion could be reached as to the effectiveness of early versus d ... | 1993 | 8148139 |
| rhodococcus equi-associated osteomyelitis in foals. | two cases of rhodococcus equi infection in foals are described, in which osteomyelitis was a feature. because rhodococcal infection is usually low grade and chronic, and because the signs of early metaphysitis can be subtle, any articular or periarticular swelling in a foal from a farm with a history of rhodococcosis should be strongly suspected to be associated with r equi until proven otherwise. | 1993 | 8216097 |
| combination antibiotic treatment with clarithromycin for human immunodeficiency virus-associated rhodococcus equi infection. | 1993 | 8218702 | |
| spectroscopic characterization of a newly isolated cytochrome p450 from rhodococcus rhodochrous. | cytochrome p450 (p450) from rhodococcus rhodochrous have been characterized through circular dichroism and nuclear magnetic resonance (nmr) spectroscopy, both in the substrate-free and substrate-bound forms. the data are compared with those of p450cam and indicate a close similarity of the structure of the active site in the two proteins. the substrate-free species contains low-spin iron(iii), while the 2-ethoxyphenol bound species contains high-spin iron(iii). the substrate is in slow exchange ... | 1993 | 8218905 |
| the giant linear plasmid phg207 from rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini. | as described previously, in rhodococcus sp. (formerly nocardia opaca) strains mr11 and mr22, the ability to grow as an aerobic hydrogen bacterium (the aut character) is located on giant conjugative linear plasmids--on phg201 (270 kb) in strain mr11 and on phg205 (280 kb) in strain mr22. in an autotrophic transconjugant originating from mr22 a smaller plasmid, phg207 (225 kb), was detected and shown to be a recombination product of the wild-type plasmids phg204 and phg205. a donor carrying phg207 ... | 1993 | 8245832 |
| a simple chemical test to distinguish mycobacteria from other mycolic-acid-containing actinomycetes. | two hundred and fifty-two representatives of the general corynebacterium, gordona, mycobacterium, nocardia, rhodococcus and tsukamurella were degraded by alkaline hydrolysis and their mycolic acids extracted as methyl esters following phase-transfer-catalysed esterification. when the mycolic acid methyl esters were treated with a mixture of acetonitrile and toluene all mycobacterial mycolates formed copious white precipitates whereas all but 5 out of the 106 non-mycobacterial mycolates remained ... | 1993 | 8245845 |
| association of microbiologic flora with clinical, endoscopic, and pulmonary cytologic findings in foals with distal respiratory tract infection. | undifferentiated distal respiratory tract disease (nasal discharge, cough, pneumonia) in foals (1 to 8 months old) is a burdensome economic problem on breeding farms; yet, the infective agents associated with these episodes have not been well described. possible causes of these episodes of illness were investigated by culturing specimens of proximal and distal airways of clinically diseased foals (n = 101), prior to any treatment, for aerobic and anaerobic bacteria and viruses (rhinoviruses, equ ... | 1993 | 8250386 |
| novel gangliosides containing the sialyl-le(a) structure from a human rectal adenocarcinoma. | oligosaccharides were released from the gangliosides of a human rectal adenocarcinoma with endoglycoceramidase (rhodococcus sp. g-74-2) and then purified by affinity chromatography on a column of an immobilized monoclonal antibody, msw 113. structural studies, involving 600-mhz 1h nmr spectrometry, indicated the structures of these compounds to be as follows. [formula: see text] three of these oligosaccharides, 2, 3, and 4, are novel as to ganglioside sugar chains and contain both lacto series t ... | 1993 | 8253783 |
| monoclonal antibody specific to virulence-associated 15- to 17-kilodalton antigens of rhodococcus equi. | virulent rhodococcus equi produces 15- to 17-kda surface protein antigens. these antigens are used as markers to identify virulent r. equi isolates from foals and their environment by western blot (immunoblot) analysis with naturally infected foal serum. in the present study, a monoclonal antibody (mab; 10g5) was generated against the 15- to 17-kda antigens excised from sodium dodecyl sulfate-polyacrylamide gels to develop sensitive and specific immunoblot assays for the identification of virule ... | 1993 | 8253982 |
| bronchointerstitial pneumonia and respiratory distress in young horses: clinical, clinicopathologic, radiographic, and pathological findings in 23 cases (1984-1989). | twenty-three foals, between 1 and 7 months old, with signs of acute respiratory distress, were examined at the veterinary medical teaching hospital (vmth), university of california, davis, between 1984 and 1989. characteristic features included sudden onset of severe respiratory distress and tachypnea, cyanosis unresponsive to nasal oxygen, pyrexia, hypoxemia, hypercapneic respiratory acidosis, poor response to treatment, and histopathologic lesions of bronchiolitis and bronchointerstitial pneum ... | 1993 | 8263846 |
| n-terminal amino acid sequence of mutant strain brevibacterium sp. adipamidase. | the adipamidase of a mutant strain brevibacterium sp. r312 involved in the degradation of adiponitrile to adipic acid was purified. its n-terminal amino acid sequence was shown to be identical to brevibacterium sp. r312 enantio selective amidase and rhodococcus sp. n-774 amidase. | 1993 | 8274001 |
| major structural features of the cell wall arabinogalactans of mycobacterium, rhodococcus, and nocardia spp. | the cell wall arabinogalactans of strains of mycobacterium, rhodococcus, and nocardia were per-o-methylated, partially hydrolyzed with acid, and the resulting oligosaccharides were reduced and per-o-ethylated to yield per-o-alkylated oligoglycosyl alditol fragments. analyses of these fragments by gas chromatography-mass spectrometry and of the intact solubilized polysaccharides by 1h and 13c nmr revealed the major structural features of the different arabinogalactans from representatives of the ... | 1993 | 8275507 |
| bacterial pneumonia in hiv-infected patients. | individuals infected with the human immunodeficiency virus (hiv) are more susceptible to bacterial infections because of defects in both cellular and humoral immunity. streptococcus pneumoniae and haemophilus influenzae are the most common causes of bacterial pneumonia in hiv-infected patients. however, more unusual bacteria can also cause pneumonia. response to therapy is generally good for infections caused by pyogenic organisms, and complications are relatively few. unfortunately, infections ... | 1993 | 8278678 |
| rhodococcus equi pneumonia: case report and literature review. | to present a case of rhodococcus equi (re) pneumonia and discuss its pathophysiology and treatment. | 1993 | 8286804 |
| spheroplast formation and plasmid isolation from rhodococcus spp. | the genus rhodococcus comprises aerobic gram-positive actinomycetes that show considerable morphological and metabolic diversity and are known to be involved in the development of plant diseases and degradation of environmental pollutants. we describe a method for cell lysis and large plasmid dna isolation from rhodococcus by creating lysozyme susceptible cells by predigestion with the enzyme mutanolysin. mutanolysin action resulted in the liberation of reducing sugars and free amino acids from ... | 1993 | 8292332 |
| restriction map of a virulence-associated plasmid of rhodococcus equi. | the restriction cleavage map of a virulence-associated plasmid preat701 of rhodococcus equi was constructed with ecori and hindiii by means of cloning the restriction fragments, cross southern hybridization with each fragment, and hybridization with probes generated by modified inverse pcr. the genetic region responsible for expression of virulence-associated 15- to 17-kilodalton antigens was determined. | 1993 | 8302941 |
| [detection of rhodococcus equi antibodies in human serum]. | the authors investigated in sera of patients with pneumonia the presence of antibodies against the equi factor rhodococcus equi. for examination they used agar immunodiffusion and a test for neutralizing the equi factor. some 110 sera of different donors were examined. in agar immunodiffusion they used the equi factor as antigen either in the separated form or equi factor produced by the growing strain of r. equi. the neutralization test was implemented on agar media with red blood cells sensiti ... | 1993 | 8306394 |
| chronic postoperative endophthalmitis following cataract extraction and intraocular lens implantation. report on nine patients. | between 1987 and 1992, nine patients (three men and six women; mean age, 75 years) presented with chronic postoperative endophthalmitis after cataract extraction and intraocular lens (iol) implantation. eight patients were referred from another institution. the interval between iol implantation and the beginning of intraocular inflammation averaged 5.6 months (range, 1-19 months). the referral diagnosis was "toxic lens" syndrome in seven patients. the main clinical findings were a hypopyon in th ... | 1993 | 8312828 |
| [recurrent abscess of the lung caused by rhodococcus equi in an hiv-positive patient: response to the combination imipenem/vancomycin]. | 1993 | 8315968 | |
| new method for the isolation of polyglycosylceramides from human erythrocyte membranes. | a new procedure was developed for the isolation of long-chain, highly polar glycosphingolipids from human erythrocytes. the membrane material left after extraction of membrane lipids with organic solvents was peracetylated in a mixture of formamide, pyridine and acetic anhydride, and the acetylated products were then extracted with chloroform. the material was fractionated and purified by means of sephadex lh-20, sephadex lh-60 and silica-gel chromatography. the final preparations were mixtures ... | 1993 | 8323973 |
| progressive pulmonary infiltrates and positive blood cultures for weakly acid-fast, gram-positive rods in a 76-year-old woman. | 1993 | 8325080 | |
| metabolism of the herbicide atrazine by rhodococcus strains. | rhodococcus strains were screened for their ability to degrade the herbicide atrazine. only rhodococci that degrade the herbicide eptc (s-ethyl-dipropylthiocarbamate) metabolized atrazine. rhodococcus strain te1 metabolized atrazine under aerobic conditions to produce deethyl- and deisopropylatrazine, which were not degraded further and which accumulated in the incubation medium. the bacterium also metabolized the other s-triazine herbicides propazine, simazine, and cyanazine. the n dealkylation ... | 1993 | 8328812 |
| three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the gram-positive polychlorobiphenyl-degrading bacterium rhodococcus globerulus p6. | rhodococcus globerulus p6 (previously designated acinetobacter sp. strain p6, arthrobacter sp. strain m5, and corynebacterium sp. strain mb1) is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. the genetic and biochemical analyses of the pcb catabolic pathway reported here have revealed the existence of a pcb gene cluster--bphbc1d--and two further bphc genes--bphc2 and bphc3--that encode three narrow-substrate-specificity enzymes (2,3-dihydroxybiphenyl dioxygenases) that ... | 1993 | 8335622 |
| pharmacokinetics of single intravenous and single and multiple dose oral administration of rifampin in mares. | the disposition of rifampin in six healthy mares after single intravenous (i.v.) and oral (p.o.) doses and after seven oral doses of 10 mg/kg administered twice a day was investigated using a high performance liquid chromatographic (hplc) method. pharmacokinetic variables for rifampin determined using the hplc method were comparable to variables reported from earlier studies utilizing a microbiological assay. desascetylrifampin, a major metabolite of the parent compound, could not be detected in ... | 1993 | 8345562 |
| virulence-associated plasmids in rhodococcus equi. | twenty-three strains of rhodococcus equi from independent clinical cases were analyzed for the presence of virulence plasmid dna. of the clinical isolates, 19 contained an 85-kb plasmid and the remaining 4 contained a 90-kb plasmid. all of the isolates expressed 15- to 17-kda antigens and were virulent in mice. restriction enzyme and southern blot analyses showed large regions of dna homology between the 85- and 90-kb virulence plasmids. it was concluded tentatively that there are at least two v ... | 1993 | 8349748 |
| in-vitro susceptibility to antimicrobial drugs of bacterial isolates from horses in the netherlands. | minimum inhibitory concentrations (mics) of 30 antimicrobial agents (including the hitherto unreported antimicrobial agents doxycycline, minocycline, vanomycin, 3 quinolones and 3 combinations of antimicrobial agents) for isolates of salmonella spp. (20), escherichia coli (17), klebsiella spp. (8), proteus spp. (7), pseudomonas aeruginosa (7), actinobacillus equuli (5), rhodococcus equi (4), streptococcus zooepidemicus (23), streptococcus equisimilis (6), streptococcus equi (4), coagulase-positi ... | 1993 | 8354217 |
| rhodococcus equi. | this article summarizes recent advances in understanding of the epidemiology, pathogenesis, clinical and laboratory diagnosis, immunology, treatment, and control of rhodococcus equi infections in foals. our understanding of these aspects currently is sufficient to ensure control of this problem on affected farms and in infected foals. more information, however, is needed on factors predisposing foals to r. equi pneumonia, in particular, the nature of the naturally occurring cellular immunodefici ... | 1993 | 8358650 |
| chromatographic and mass spectrometric characterization of 3-o-benzoyl methyl ester derivatives of mycolic acid fractions from corynebacterium pseudotuberculosis, c. diphtheriae and rhodococcus rhodochrous. | a benzoyl group was attached to the 3-hydroxyl group of the methyl ester derivative of corynomycolic acid fraction isolated from corynebacterium pseudotuberculosis. the infrared spectrum of the 3-o-benzoylated compound displayed a series of characteristic absorptions found at 1110, 1267 and 1603 cm-1 that confirmed the presence of a monosubstituted phenyl grouping. the 1h-nmr spectrum showed peaks representing protons of the aromatic ring at 7.4 ppm and 8.0 ppm. the uv spectrum revealed two abso ... | 1993 | 8358854 |
| cholesterol side-chain cleavage by immobilized cells of rhodococcus equi dsm 89-133. | side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of r. equi dsm 89-133 immobilized in polyacrylamide gel. in batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. maximal activity of the immobilized cells was 60 to 70% of t ... | 1993 | 8359831 |
| penicillin-binding proteins of rhodococcus equi: potential role in resistance to imipenem. | rhodococcus equi is a gram-positive coccobacillus which, like other members of the order actinomycetales, is increasingly reported as an opportunistic pathogen in patients with aids. the use of combinations of antibiotics that include imipenem (imp) has been suggested for the treatment of patients infected with r. equi. an antagonism between imp, meropenem, cefoxitin, ceftriaxone, moxalactam, and oxacillin and other beta-lactams, such as penicillin, amoxicillin, cephalothin, and ticarcillin, was ... | 1993 | 8363366 |
| rhodococcus equi pneumonia in a patient with aids: radiologic features. | 1993 | 8372786 | |
| nicotinoprotein [nad(p)-containing] alcohol/aldehyde oxidoreductases. purification and characterization of a novel type from amycolatopsis methanolica. | extracts of gram-positive bacteria like rhodococcus rhodochrous, rhodococcus erythropolis and amycolatopsis methanolica, but not those of several gram-negative ones, showed dehydrogenase activity for ethanol as well as for methanol when 4-nitroso-n,n-dimethylaniline (ndma) was used as electron acceptor. chromatography of extracts of the first two organisms revealed one activity for both substrates, that of a. methanolica two activities, one of which is able to oxidize methanol and has been purif ... | 1993 | 8385013 |
| a cancer patient with central venous catheter-related sepsis caused by tsukamurella paurometabolum (gordona aurantiaca) | 1993 | 8399886 |