Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| immunotherapy of guinea pigs with a transplanted hepatoma: comparison of intralesionally injected emulsions containing heat-killed nocardia rubra, mycobacterium bovis (bcg) and mycobacterium phlei. | heat-killed cells of mycobacterium bovis (bcg), mycobacterium phlei and nocardia rubra were each tested in emulsified form for their ability to cause regression of established dermal transplants and lymph node metastases of a syngeneic hepatocarcinoma in guinea pigs. on a weight basis, bcg was superior to n. rubra in causing tumor regression. under the conditions tested n. rubra was inferior to m. phlei in its antitumor activity. m. phlei and bcg were approximately the same in their therapeutic ... | 1982 | 7099513 |
| enzymatic synthesis, characterization, and metabolism of the coenzyme a ester of o-succinylbenzoic acid, an intermediate in menaquinone (vitamin k2) biosynthesis. | the enzymatic synthesis of the "active" o-succinylbenzoic acid is described and the factors controlling its formation are investigated. tritium-labeled coenzyme a is incorporated into "active" o-succinylbenzoic acid, but label from [2-3h]atp or [gamma-32p is not, indicating that the active compound is a coenzyme a thio ester(2). the compound is shown by two different methods to contain 1 mol only of coenzyme a per mol of o-succinylbenzoic acid. the o-succinylbenzoic and coenzyme a ester (2) is u ... | 1982 | 7045104 |
| [characteristics of the immobilization and transformation of sterols by mycobacterium phlei 1026 cells incorporated into paag]. | experiments were carried out to study the effect of polymerization conditions and polyacrylamide gel structure on the enzyme activity of mycobacterium phlei immobilized cells degrading sterols to c19-steroids. the modified sterol 4-chlostene-3(o-carboxymethyl)oxime was used as substrate. the polymerization conditions (the number of the cells incorporated and the reaction temperature) significantly affected the ability of the immobilized cells to degrade the sterol side chain. the monomer contact ... | 1983 | 6878209 |
| dna-methylating activity of mycobacteria. | the methylating activity of the four mycobacterium strains mycobacterium phlei, mycobacterium smegmatis strain butyricum, mycobacterium smegmatis strain rabinowitz, lysogenic mycobacterium smegmatis strain rabinowitz was studied in vitro. all the four strains were found to have methylating activity; enzyme containing extracts of m. smegmatis strain butyricum and m. phlei showed a stronger activity than those of m. smegmatis strain rabinowitz and the lysogenic rabinowitz strain. the methylases of ... | 1983 | 6659854 |
| interaction of lanthanide cations and uranyl ion with the calcium/proton antiport system in mycobacterium phlei. | uranyl ions (uo2+(2)) and lanthanide cations (la3+, nd3+, sm3+, eu3+, tb3+ and dy3+) at 100-200 microm concentration inhibited active transport of ca2+, mediated by respiratory linked substrates as well as by atp hydrolysis, without affecting respiration and membrane-bound atpase activity, in inside-out membrane vesicles of mycobacterium phlei. the extent of inhibition in the uptake of ca2+, mediated by atp hydrolysis, increased with increase in ionic radii of these cations. lanthanide cations d ... | 1983 | 6838872 |
| incorporation of thymine, thymidine, adenine and uracil into nucleic acids of mycobacterium phlei and its phage. | like other prototroph bacteria, mycobacterium phlei was found to incorporate thymine into its dna very poorly. as a result of a rapid thymidine-to-thymine conversion, thymidine incorporation also proved to be inefficient. thymidine incorporation could be somewhat enhanced by pretreatment of the cells with uridine. when radioactive adenine, and particularly uracil, were used as precursors, highly labelled dna could be obtained from the cells, although the majority of radioactivity was found in th ... | 1983 | 6847034 |
| purification and functional properties of the dccd-reactive proteolipid subunit of the h+-translocating atpase from mycobacterium phlei. | interaction of n,n'-dicyclohexylcarbodiimide (dccd) with atpase of mycobacterium phlei membranes results in inactivation of atpase activity. the rate of inactivation of atpase was pseudo-first order for the initial 30-65% inactivation over a concentration range of 5-50 microm dccd. the second-order rate constant of the dccd-atpase interaction was k = 8.5 x 10(5) m-1 x min(-1). the correlation between the initial binding of [14c]dccd and 100% inactivation of atpase activity shows 1.57 nmol dccd b ... | 1983 | 6221756 |
| studies on the mechanism of action of local anesthetics on proton translocating atpase from mycobacterium phlei. | we have measured the inhibitory potencies of local anesthetics (procaine, lidocaine, tetracaine and dibucaine) on atp-mediated h+-translocation, ca2+-transport and atpase activity in membrane vesicles from mycobacterium phlei. procaine and lidocaine up to 1 mm concentration did not inhibit atp-dependent h+-translocation, ca2+-transport and atpase activity. however, tetracaine and dibucaine at 0.2 mm concentration caused dissipation of the proton gradient, measured by the reversal of the quenchin ... | 1984 | 6231050 |
| deacylation of acetylated amino acids by the esterase from mycobacterium phlei. | in addition to typical acetyl esters, acetic ester acetyl-hydrolase from mycobacterium phlei can degrade aliphatic acetylated amino acids under suitable conditions. | 1984 | 6745815 |
| growth of group iv mycobacteria on medium containing various saturated and unsaturated fatty acids. | seventy-one strains of 15 species of rapidly growing mycobacteria were studied for their susceptibilities to fatty acids with 2 to 20 carbons by the agar dilution method at ph 7.0. most mycobacteria other than potential pathogens (mycobacterium fortuitum and mycobacterium chelonei) were resistant to saturated fatty acids, except for lauric acid (c12:0) (mic, 6.25 to 25 micrograms/ml) and capric acid (c10:0) (mic, 50 to 100 micrograms#ml). m. fortuitum and m. chelonei were substantially insuscept ... | 1984 | 6486760 |
| cell cycle in mycobacterium phlei. | the initial replication region of the chromosome on the replication map of m. phlei constructed by means of sequential mutagenesis in synchronous populations was accurately determined. by following the time shift of the replication moment of the genes bac and met in the control culture and in the culture with the initial inhibition of dna synthesis by nalidixic acid the start of replication of the chromosome was determined at 15 min before replication of the gene ile. on the basis of the results ... | 1984 | 6378738 |
| absence of mycobactin in mycobacterium leprae; probably a microbe dependent microorganism implications. | ferric mycobactins were prepared from mycobacterium phlei. mycobacterium avium--intracellulare a and h, isolated respectively from armadillo and human leprosy specimens. attempts were made to extract mycobactin from host grown m. leprae cells. the crude ferric mycobactin extracts were tested for growth supporting effect on the mycobactin dependent m. paratuberculosis strain atcc 19698. mycobactins prepared from m. phlei and the two m. avium--intracellulare strains had growth promoting effect on ... | 1985 | 3897405 |
| nonspecific immunostimulation against viruses. | a trypsinized preparation from chromogenic selected strain of mycobacterium phlei (nsi) stimulated the recipient immune system non-specifically against a variety of viruses viz. rabies virus (rna virus). marek's disease (dna virus) and foot and mouth disease virus (rna virus) in phylogenetically different hosts like mice, chicks and guinea pigs respectively. investigation into mechanisms of such nonspecific immunostimulation revealed that there was induction of strong cell mediated immune respon ... | 1985 | 4064625 |
| [antibacterial properties of some spice plants before and after heat treatment]. | this study was carried out to understand the antibacterial properties of some spice plants before and after heat treatment in boiling water. the samples included the core and the outer layers of onion, the white and the green parts of green onion, garlic bulb, ginger, ginger root, sweet pepper, chili pepper, brown pepper, and mustard. the test microorganisms included escherichia coli, salmonella typhimurium, vibrio parahaemolyticus, pseudomonas aeruginosa, proteus vulgaris, staphylococcus aureus ... | 1985 | 4064797 |
| the use of a hydrophobic resin as a product reservoir in steroid transformations. | particles of the hydrophobic resin polydimethylsiloxane were found to preferentially accumulate steriods on the basis of their hydrophobicity. thus, the resin selectively sorped the steroid products resulting from the transformation of diosgenin by nocardia rhodochrous, with the result that higher yields of the later biotransformation product, 1-dehydrodiosgenone, and lower yields of the first product, diosgenone, were obtained than in the absence of resin. furthermore, steroids accumulated by t ... | 1985 | 18553742 |
| selective enzyme staining procedures for characterization of mycobacterial immunoprecipitates. | selective staining procedures for four different enzymes (malate dehydrogenase, glutamic oxaloacetic transaminase, leucine aminopeptidase and glucose phosphate isomerase) in combination with two-dimensional immunoelectrophoresis were successfully applied to the analysis of antigen preparations from mycobacteria. thus, a precipitate corresponding to malate dehydrogenase could e.g. be demonstrated in multi-linear precipitation patterns of mycobacterium avium, mycobacterium bovis bcg, mycobacterium ... | 1986 | 2417958 |
| step-wise isolation of rna and dna from mycobacteria. | mycobacterium phlei and m. smegmatis were lysed at -15 degrees c in the presence of guanidinium salts and poly(a)+-rna. nonpolyadenylated rna (mainly rrna) and dna were isolated in successive steps from the same lysate. dna isolated by this procedure was sufficient to yield distinct bands on treatment with restriction endonucleases as shown by hybridization to 125i-labeled rrna. the successive isolation of poly(a)+-rna, nonpolyadenylated rna, and high molecular weight dna from the same lysate by ... | 1986 | 2427627 |
| evidence for genetic divergence in ribosomal rna genes in mycobacteria. | dna was isolated from mycobacterium phlei and from m. smegmatis. each dna sample was restricted with endonucleases, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose film. fragments of dna containing rrna sequences were identified by means of 125i-labelled rrna of m. phlei or of m. smegmatis. the distributions of restriction endonuclease sites within the rrna gene(s) and flanking sequences were found to be characteristic for each of the two species. hy ... | 1986 | 3002853 |
| phenotypic changes in mycobacteria grown in oxygen-limited conditions. | laboratory strains of mycobacterium phlei, m. smegmatis, m. fortuitum, m. gordonae, m. kansasi, m. bovis, m. tuberculosis and m. intracellulare were adapted to grow in an anaerobic environment. concomitant with the transition to anaerobic growth was loss of acid-fastness, loss or modification of colonial pigmentation, and loss of ability to grow on a malachite green-containing medium. the mycobacteria grown anaerobically produced acid from a greater range of carbohydrates than aerobically grown ... | 1986 | 3084791 |
| differentiation of mycobacterium thermoresistibile from mycobacterium phlei and other rapidly growing mycobacteria. | mycobacterium thermoresistibile is the only chromogenic rapidly growing mycobacterial species reported to cause infections in humans and animals. dna-dna hybridization (s1 nuclease method) showed that m. thermoresistibile formed a dna relatedness group which was only 24 to 30% related to m. phlei. alkaline phosphatase and beta-galactosidase differentiated m. thermoresistibile from m. phlei. among the rapidly growing mycobacteria, the mycolic acid pattern of m. thermoresistibile was unique (alpha ... | 1986 | 3122636 |
| aspects of mycobacterial response to beryllate ions in vitro. | comparative growth measurements of the mycobacterial species mycobacterium phlei and mycobacterium tuberculosis were carried out with liquid cultures supplemented with oxalatoberyllate ions in the concentration range 0-120 microm be. for both species, the effect of beryllium on specific growth parameters can be represented by a be concentration-dependent, exponential expression. evidence for beryllium binding to cellular phospholipids (approximately 15% of be uptake) is provided by 31p nmr spect ... | 1986 | 2946115 |
| solubilization, purification, and characterization of succinate dehydrogenase from membranes of mycobacterium phlei. | succinate dehydrogenase (sdh) was solubilized from membranes of mycobacterium phlei by triton x-100 with a recovery of about 90%. the solubilized sdh was purified about 90-fold by sephacryl s-300, deae-cellulose, hydroxylapatite, and isoelectric focusing in the presence of triton x-100 with a 20% recovery. sdh was homogeneous, as determined by polyacrylamide gel electrophoresis in nondenaturing gels containing triton x-100. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme ... | 1986 | 3722123 |
| purification and characterization of dihydrofolate reductase from mycobacterium phlei. | the dihydrofolate reductase from mycobacterium phlei was purified and characterized; it has an mr of 15 000 and a pi of 4.8. it is competitively inhibited by both methotrexate and trimethoprim, although the affinity is less than for other bacterial dihydrofolate reductases. | 1986 | 3741386 |
| a simple technique for increased sensitivity in immunoelectrophoresis by the use of antiserum against igg. | precipitates in two-dimensional immunoelectrophoresis (2d-ie) plates can be amplified by the application of an antiserum against igg after the second dimension run. thereby, the sensitivity of the ie method is increased; faint precipitates and antigen-antibody reactions close to but below the level of forming recognizable precipitates are made visible. the advantage of the method was demonstrated for a multilinear mycobacterium phlei system. | 1987 | 3819446 |
| 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester, an intermediate in vitamin k2 (menaquinone) biosynthesis. | enzyme preparations from mycobacterium phlei, escherichia coli and galium mollugo cell suspension cultures were incubated in the presence of 4-(2'-carboxyphenyl)-4-oxobutyrate (i.e. o-succinylbenzoic acid, osb, 1), atp, coenzyme a and mg2+. the main product isolated from the incubation mixture was 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester (2) as determined by comparison with synthetic coenzyme a esters. synthetic and enzymically formed 4-(2'-carboxyphenyl)-4-oxobutyryl coenzyme a ester ... | 1987 | 2966501 |
| analysis by elisa and western blotting of antibody reactivities in cattle infected with mycobacterium paratuberculosis after absorption of serum with m phlei. | preabsorption of cattle serum with mycobacterium phlei was of value in eliminating falsely positive reactions in an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against m paratuberculosis. specific antibody titres from 16 animals naturally infected with m paratuberculosis were unaffected by absorption. analysis by western blotting indicated that a different set of antigens of m paratuberculosis were recognised by serum from falsely positive reactors compared with tha ... | 1987 | 3296062 |
| nadp-specific isocitrate dehydrogenase of mycobacterium phlei atcc 354: purification and characterization. | nadp-dependent isocitrate dehydrogenase (ec 1.1.1.42) from mycobacterium phlei atcc 354 was purified to homogeneity by ammonium sulphate fractionation, followed by deae cellulose and sephadex g-200 chromatography. the ph optimum of the enzyme was 8.5. the km values for isocitrate and nadp were 74 and 53 microm, respectively. mn2+ was essential for enzyme activity. the enzyme lost all activity on incubation at 70 degrees c for 15 min; isocitrate and nadp protected against this thermal inactivatio ... | 1987 | 3448153 |
| antimicrobial activity of benzylisoquinoline alkaloids. | the antimicrobial in vitro activity of 14 benzylisoquinoline alkaloids was investigated by agar diffusion and agar dilution methods against several genera of microorganisms that included streptococcus, staphylococcus, bacillus, lysteria, escherichia, salmonella, klebsiella, pseudomonas, enterobacter, serratia, shigella, mycobacterium and candida. anolobine was the most active compound against grampositive bacteria with mic90 between 12 and 50 mg/l; less active were anonaine, lysicamine and lirio ... | 1987 | 3615557 |
| patterns and distribution of aminoglycoside-acetylating enzymes in rapidly growing mycobacteria. | cell-free extracts from clinical, laboratory, and environmental isolates of mycobacterium fortuitum, mycobacterium smegmatis, mycobacterium phlei, and mycobacterium vaccae were tested for the presence of aminoglycoside-acetylating enzyme and compared with enzymes from gram-negative organisms. acetylating activity was detected in all strains examined despite variable levels of aminoglycoside susceptibility. substrate profiles revealed 2 different patterns of 3-n-acetyltransferase. one pattern exh ... | 1987 | 3619192 |
| isolation and characterization of the facultative methylotroph mycobacterium id-y. | a facultatively methylotrophic mycobacterium was isolated from cleveland harbor, ohio, usa. the isolate, designated id-y, used a wide range of carbon and energy sources including methane and several other hydrocarbons. it displayed a growth cycle from rod-shaped exponential-phase cells, with many cell pairs exhibiting v-formation, to cocco-bacillary stationary-phase cells. a fixation technique involving glutaraldehyde/alcian blue resulted in the observation of a three-layered cell wall. isolate ... | 1987 | 3655742 |
| influence of oxygen tension on the respiratory activity of mycobacterium phlei. | growth of mycobacterium phlei under low oxygen tension resulted in specific activities two to twenty times lower for formate dehydrogenase, malate dehydrogenase, beta-hydroxybutyrate dehydrogenase, lactate oxidase and nadh dehydrogenase than when cultures were grown under high aeration. an increase in fumarate reductase and succinate dehydrogenase occurred with m. phlei grown under low oxygen tension. malate: vitamin k dehydrogenase and glucose-6-phosphate dehydrogenase activity were not signifi ... | 1988 | 3183614 |
| isolation and structural characterization of a new non-phosphorylated lipoamino acid from mycobacterium phlei. | a new non-phosphorylated lipoamino acid was extracted from mycobacterium phlei, strain ist. it is particularly sensitive to alkaline hydrolysis, and contains a lysine residue joined to a 1,2-diglyceride via an ester linkage. the fab-positive mass spectrum shows the presence of various molecular species of which the most abundant contains a palmitic and a tuberculostearic acid residue. an analogue of this lipid was synthesized, 1,2-dipalmitoyl 3-lysyl glycerol. both its chromatographic behavior ( ... | 1988 | 3240562 |
| analysis of fatty acids by negative ion gas chromatography/tandem mass spectrometry: structural correlations between alpha-mycolic acid chains and delta-5-monounsaturated fatty acids from mycobacterium phlei. | the analysis of a complex mixture of monounsaturated fatty acids from mycobacterium phlei is achieved by capillary gas chromatographic separation of their pentafluorobenzyl esters, formation of gas-phase carboxylate anions by electron capture ionization and decomposition of these anions by collision activation. this method allows the structural determination of fatty acid isomers by examination of their collisionally activated dissociation mass-analysed ion kinetic energy spectra. a good correla ... | 1988 | 3242686 |
| [analysis of genes for ribosomal rna in mycobacterium phlei]. | 1988 | 2452272 | |
| regulation of glutamine synthetase activity by phosphorylation instead of by adenylylation. | o-phosphotyrosyl glutamine synthetase (p-gs) was isolated from highly adenylated glutamine synthetase (amp-gs) purified from mycobacterium phlei, by treatment with micrococcal nuclease. the physical characteristics of p-gs were quite similar to those of amp-gs except for the uv-absorption spectrum. in either mg2+- or mn2+-dependent biosynthetic reactions, the kinetic properties, such as optimum ph, vmax, and apparent km for each of three substrates of p-gs, were found to be in good agreement wit ... | 1988 | 2902854 |
| induction of cell mediated immune response by nonspecific stimulator of immunity against antigenically unrelated oncogenic virus (marek's disease virus). | an enzyme treated preparation of mycobacterium phlei (nsi), induced strong cell mediated immune response (cmir) against specific as well as against nonspecific oncogenic marek's disease (mdv) in birds, as evinced by lymphocyte migration inhibition, (lmit) lymphocyte transformation test (lt) and lymphokine (lymphocyte migration inhibition factor) lyif assay. maximum cmir could be observed towards third week post inoculation. all the three tests exhibited a positive correlation. such phenomenon of ... | 1989 | 2479599 |
| comparison of dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. | a rapid, simple and inexpensive dot immunobinding assay (dia) was evaluated for the serodiagnosis of paratuberculosis in cattle. the assay was performed on nitrocellulose strips which were dotted with purified protoplasmic antigen of mycobacterium paratuberculosis. after incubation with test serum samples, the bound antibodies were detected using an enzyme-amplified immunostaining procedure. the efficacy of dia as a screening test for paratuberculosis was compared to that of an enzyme-linked imm ... | 1989 | 2512004 |
| in vitro antibacterial effect of the essential oil of thymus longiflorus boiss. | the essential oil of thymus longiflorus boiss was tested for its in vitro antibacterial activity. the results showed antibacterial effects against gram-positive and gram-negative bacteria, especially against pseudomonas fluorescens and mycobacterium phlei. | 1989 | 2514339 |
| nonspecific immunoprotection against rous sarcoma tumor in chicks. | an enzyme treated preparation of saprophytic mycobacterium phlei, referred as nsi, when administered intramuscularly has been found to protect the chicks against rous sarcoma virus induced tumor. a protection level of 35.4%, 24.1% and 21.2% were observed when challenged on 10th, 20th and 30th day post nsi inoculation. the tumor growth inhibitory-activity of nsi was significant (p less than 0.01). both, systemic and intralesional administration of nsi exhibited significant tumorostatic activity ( ... | 1989 | 2555299 |
| physical and chemical characterization of glutamine synthetase purified from mycobacterium phlei. | glutamine synthetase (l-glutamate: ammonia ligase [adp forming]) [ec 6.3.1.2] has been purified from a gram-positive, acid-fast bacterium, mycobacterium phlei, by simple procedures with 57% recovery. the enzyme resembled that from mycobacterium smegmatis in the subunit size (56,000), molecular weight (670,000), amino acid composition, the amino acid sequence of the nh2-terminal, and the secondary structure. the enzyme activity was regulated by adenylylation of each subunit in the dodecameric mol ... | 1989 | 2569461 |
| trehalose dimycolate enhances resistance to infection in neutropenic animals. | bacterial infections are lethal complications of neutropenia, and antibiotics alone are inadequate therapy for these infections. irradiated mice become severely neutropenic and remain susceptible to infection for 2 to 3 weeks, depending on the dose and quality of radiation. some bacterial cell wall derivatives stimulate nonspecific host defense mechanisms against a variety of microbes which might cause postirradiation infection. in this study we determined if the cell wall glycolipid trehalose d ... | 1989 | 2663726 |
| mycolic acid metabolic filiation and location in mycobacterium aurum and mycobacterium phlei. | synchronous cultures of mycobacterium aurum were used to prove a close relationship between cellular division and active synthesis of mycolic acids (characteristic long-chain 3-hydroxyacids, branched at position 2), confirming previous proposals. mycolic acid biosynthesis was studied in two species (mycobacterium phlei and m. aurum) each producing three types of mycolic acids: di-unsatured mycolates, oxomycolates and wax-ester mycolates (ester of dicarboxymycolic acid and 2-icosanol or 2-octadec ... | 1989 | 2714297 |
| septic arthritis due to mycobacterium phlei presenting as infantile reiter's syndrome. | we describe a 7-year-old peruvian boy who presented with a 2 week history of conjunctivitis, urethritis and arthritis, in whom mycobacterium phlei was the only organism repeatedly isolated from synovial fluid and tissue and who responded to conventional antituberculous therapy. to our knowledge this is the first documented case of human disease caused by this microorganism. | 1989 | 2810265 |
| a cloned dna probe for the detection of mycobacterium paratuberculosis. | dna extracted from mycobacterium paratuberculosis, which had been isolated from a cow with clinical johne's disease, was used to make a gene library in the escherichia coli expression vector phage lambda gt11. plaque-lifts were made from the library onto nitrocellulose membranes. these were screened by differential hybridization using radiolabelled chromosomal dna from m. paratuberculosis and mycobacterium phlei. by this method six recombinants that hybridized to m. paratuberculosis but not to m ... | 1989 | 16031516 |
| mechanism of gram variability in select bacteria. | gram stains were performed on strains of actinomyces bovis, actinomyces viscosus, arthrobacter globiformis, bacillus brevis, butyrivibrio fibrisolvens, clostridium tetani, clostridium thermosaccharolyticum, corynebacterium parvum, mycobacterium phlei, and propionibacterium acnes, using a modified gram regimen that allowed the staining process to be observed by electron microscopy (j. a. davies, g. k. anderson, t. j. beveridge, and h. c. clark, j. bacteriol. 156:837-845, 1983). furthermore, since ... | 1990 | 1689718 |
| the sensitivity and specificity of a modified elisa for the diagnosis of johne's disease from a field trial in cattle. | an enzyme-linked immunosorbent assay (elisa) for the detection of antibodies against mycobacterium paratuberculosis in cattle was evaluated in three herds known to have johne's disease. prior to testing, the plasma was absorbed with dried mycobacterium phlei in order to remove cross-reacting antibody specificities. the sensitivity and specificity of the elisa were calculated after repeatedly testing 327 cattle in the infected herds. of these, 53 animals had one or more positive faecal cultures o ... | 1990 | 2281604 |
| o-succinylbenzoate: coenzyme a ligase, an enzyme involved in menaquinone (vitamin k2) biosynthesis, displays broad specificity. | o-succinylbenzoate: coenzyme a ligase, an enzyme involved in menaquinone biosynthesis, was purified from mycobacterium phlei and characterized with respect to isoelectric point, molecular weight, ph optimum, temperature optimum and kinetic data. the enzyme hydrolyses atp to amp. the substrate and cofactor specificity of the enzyme was tested with analogues of o-succinylbenzoic acid, different nucleotides, thiols and divalent cations. the enzyme appears to possess broad specificity for substrates ... | 1991 | 1663748 |
| interferon gamma fails to activate human monocyte-derived macrophages to kill or inhibit the replication of a non-pathogenic mycobacterial species. | the ability of interferon gamma (gamma) to activate human macrophages to kill mycobacteria was investigated using a mycobacterial species that does not cause disease in man. although interferon activated human macrophage activity against other intracellular parasites, toxoplasma gondii and listeria monocytogenes, it failed to activate human monocyte-derived macrophages to kill not only mycobacterium tuberculosis but also the non-pathogenic species, mycobacterium phlei. | 1991 | 1813779 |
| evaluation of enzyme-linked immunosorbent assay in comparison with complement fixation test for the diagnosis of subclinical paratuberculosis in cattle. | an enzyme-linked immunosorbent assay (elisa) was evaluated and compared in parallel with the standard complement fixation test (cft) for the diagnosis of bovine subclinical paratuberculosis. bovine sera preabsorbed with the mixture of mycobacterium phlei and kaolin suspension were assayed for antibody activities to the crude protoplasmic antigen of mycobacterium paratuberculosis in the elisa. elisa antibody titer was expressed as elisa antibody index (eai) value: eai = (at-an)/(ap-an), where at, ... | 1991 | 1834198 |
| nonsporulating bacterial species contain dna sequences homologous to the bacillus spore-specific c-protein gene. | genes for small, acid-soluble spore proteins (sasps) are ubiquitous among the spore-forming bacteria and are expressed only during sporulation. although they perform the function of amino acid storage in spores, the members of the sasp-c multigene family probably serve additional functions, so that similar sequences might be present in non-spore-formers. using the sasp-c gene (ssp-c) as a hybridization probe, restriction digests of whole genomic dna from seven nonsporulating bacterial species we ... | 1991 | 1848527 |
| tumor necrosis factor alpha mediates lethal activity of killed gram-negative and gram-positive bacteria in d-galactosamine-treated mice. | treatment with d-galactosamine increases sensitivity of lipopolysaccharide (lps)-responder mice to the lethal effects of lps, while nonresponder mice remain resistant (m.a. freudenberg, d. keppler, and c. galanos, infect. immun. 51:891-895, 1986). in the present study it is shown that, in contrast to lps, killed gram-negative bacteria (salmonella abortus equi and s. typhimurium) were highly toxic for d-galactosamine-treated lps-responder (c57bl/10 scsn and c3h/hen) and -nonresponder (c57bl/10 sc ... | 1991 | 2037372 |
| immunization against anthrax with bacillus anthracis protective antigen combined with adjuvants. | the protective efficacy of immunization against anthrax with bacillus anthracis protective antigen (pa) combined with different adjuvants was tested in hartley guinea pigs and cba/j and a/j mice. adjuvant components derived from microbial products that were tested included threonyl-muramyl dipeptide (threonyl-mdp); monophosphoryl lipid a (mpl); trehalose dimycolate (tdm); and the delipidated, deproteinized, cell wall skeleton (cws) from either mycobacterium phlei or the bcg strain of mycobacteri ... | 1992 | 1730501 |
| correlation between calmodulin-like protein, phospholipids, and growth in glucose-grown mycobacterium phlei. | in mycobacterium phlei tmc 1548 supplementation of growth medium containing 2% v/v glycerol with glucose (up to 5% w/v) resulted in an increase in growth (yield of cells), in amount of total phospholipids, and in each of the individual phospholipids (cardiolipin, phosphatidylethanolamine, phosphatidylinositol and its mannosides, and phosphatidylglycerol). however, when the medium was supplemented with a higher concentration (7.5% w/v) of glucose, both growth and phospholipid levels decreased to ... | 1992 | 1319278 |
| polyclonal b-lymphocyte activation of sheep by mycobacterium phlei against sheep pox virus. | a trypsinized preparation of mycobacterium phlei, non specific stimulator of immunity (nsi), and sheep pox virus (spv) were inoculated in different groups of sheep to activate b-lymphocytes and induce spv neutralizing substance(s). nsi sensitized sheep b-lymphocytes in the presence of nsi or lymphokine elaborated spv neutralizing substance(s). the spv sensitized b-lymphocytes also mediated such neutralizing substance(s). healthy control sheep b-lymphocytes failed to show any appreciable amount o ... | 1992 | 1324887 |
| enhanced production of antibody with specific antigen. | on inoculation of nonspecific stimulator of immunity (nsi), prepared from mycobacterium phlei (m. phlei), simultaneously along with sheep pox virus (spv) in sheep, the recipient has exhibited appreciable level of spv specific antibody as early as on 10th day which reached at peak level on 20th day and remained unaltered on 30th day of postimmunisation as evinced by serum neutralisation test (snt), enzyme linked immunosorbant assay (elisa) indirect, fluorescent antibody technique (fat) indirect, ... | 1992 | 1325943 |
| use of restriction fragment length polymorphisms resolved by pulsed-field gel electrophoresis for subspecies identification of mycobacteria in the mycobacterium avium complex and for isolation of dna probes. | mycobacterial strains from the mycobacterium avium complex were compared with each other and with mycobacterium phlei isolates by restriction endonuclease digestion of chromosomal dna with sspi and analysis by pulsed-field gel electrophoresis. characteristic profiles were observed for known typed strains, and five groups were identified. primary bovine isolates identified as mycobacterium paratuberculosis by classical methods were shown to fall into both the m. paratuberculosis- and m. avium-lik ... | 1992 | 1352787 |
| comparison of subjective and objective test evaluations for use of mycobacterium phlei-adsorbed serum in a dot-enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in cattle. | use of a dot-elisa with serum adsorbed with mycobacterium phlei or with nonadsorbed serum was compared. in addition, results attained using visual observation were compared with those obtained using a densitometer. infection status of cattle was determined by results of culture of feces from a number of cattle with various degrees of exposure (low prevalence and test-negative) and disease manifestation (clinical suspect vs subclinical infection). two paratuberculosis-negative herds, fecal cultur ... | 1992 | 1510315 |
| efficacy of various spray disinfectants on irreversible hydrocolloid impressions. | this study evaluated the effectiveness of eight disinfectant sprays on irreversible hydrocolloid impressions contaminated with three microorganisms (staphylococcus aureus, mycobacterium phlei, or bacillus subtilis) or mixed oral flora. alcide ld, omc ii, biocide, and professional lysol spray were relatively ineffective under the test conditions. sporicidin and 0.525% sodium hypochlorite were able to effect a 4-log10 (99.99%) reduction against s aureus only. a 4-log10 reduction in bacterial count ... | 1992 | 1520443 |
| diagnostic accuracy of a mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows. | the purpose of this study was to describe the responses of sera from five groups of cattle to an enzyme-linked immunosorbent assay (elisa) for paratuberculosis by using serum absorbed with mycobacterium phlei at a single working dilution. the infection status of the cattle was determined by fecal culture. cattle with different levels of exposure (high versus low prevalence and test negative) and disease manifestation (clinically suspect infection versus subclinical infection) were examined, as f ... | 1992 | 1551978 |
| use of a dot enzyme-linked immunosorbent assay on absorbed sera for the diagnosis of bovine paratuberculosis. | this study describes the response of cattle to a dot enzyme-linked immunosorbent assay (elisa) using sera absorbed with mycobacterium phlei. results obtained by visual observation are compared with those obtained using a densitometer. infection status of cattle was determined by faecal culture. cattle of different levels of exposure and disease manifestation were examined. a significantly higher dot elisa response was observed (using both absorbed and non-absorbed sera) in animals with heavy she ... | 1993 | 8400397 |
| use of an enzyme-linked immunosorbent assay for serodiagnosis of clinical paratuberculosis in goats. study by western blotting of false-positive reactions. | an enzyme-linked immunosorbent assay (elisa) was performed for diagnosis of paratuberculosis in goats, using as antigen a protoplasmic extract (ppa-3). the test was developed on the basis of the results obtained with two serum reference pools, positive and negative respectively. to avoid day-to-day variations, dilutions of the positive serum pool were included in each plate to obtain an arbitrary system, transforming absorbance into immunoglobulin (ig) g anti-mycobacterium paratuberculosis units ... | 1993 | 8400398 |
| effect of growth temperature on the fatty acid composition of mycobacterium phlei. | in mycobacterium phlei, fatty acid unsaturation increased with decreasing temperature. the 10-hexadecenoic acid content increased as the temperature was reduced from 35 degrees c to 26-20 degrees c. at lower temperatures tuberculostearic acid decreased while oleic and linoleic acids increased, the latter being found in m. phlei for the first time. concomitantly palmitic acid content decreased, and the 6- and 9-hexadecenoic acids increased slightly on reducing the temperature from 35 to 10 degree ... | 1993 | 8439233 |
| differentiation of mycobacteria on the basis of chemotype profiles by using matrix solid-phase dispersion and thin-layer chromatography. | because of the rising incidence of clinical mycobacterial infections and the difficulty in identification and characterization of mycobacteria at the subspecies and serovar levels, a technique for differentiation that could be performed quickly and with relatively little equipment and expense was developed. lysis and fractionation of mycobacteria by matrix solid-phase dispersion followed by thin-layer chromatography were used to produce chemotype profiles of the lipid and glycolipid components o ... | 1993 | 8458954 |
| utilization of beet molasses for riboflavin production by mycobacterium phlei. | mycobacterium phlei was tested for its ability to utilize beet molasses as the sole carbon source and produce riboflavin. the crude beet molasses was analyzed and treated in various ways to reduce its heavy element content and to remove the muddy residue. promising amounts of riboflavin were produced when the organism was cultivated on decationized (resin-treated) beet molasses. the highest vitamin productivity was achieved by incubating the inoculated medium containing 9% molasses and initially ... | 1994 | 8071802 |
| comparison of the 23s ribosomal rna genes and the spacer region between the 16s and 23s rrna genes of the closely related mycobacterium avium and mycobacterium paratuberculosis and the fast-growing mycobacterium phlei. | the 23s rdna sequences of mycobacterium paratuberculosis, m. avium and m. phlei and the sequences of the spacer regions between the 16s and 23s rrna genes were determined. the overall 23s rdna sequence identity between m. paratuberculosis and m. avium was 99.7% (nine mismatches), showing the very close relatedness of these mycobacteria. evolutionary distances between the five known mycobacterial 23s rrna/rdna sequences and those of other gram-positive g + c-rich bacteria were determined. the 23s ... | 1994 | 8025676 |
| mycobacterium phlei infection of the foot: a case report. | a case of mycobacterium phlei infection in the flexor digitorum longus and posterior tibialis tendon of an otherwise healthy adult male is reported. to our knowledge, this is the second reported case in the english literature of human infection by m. phlei. diagnosis and treatment of nontuberculous mycobacterial infection are discussed. the clinician is encouraged to include this infection in the differential diagnosis of pain and swelling in the extremity. | 1994 | 7894643 |
| comparison of a complement fixation test, a gel diffusion test and two absorbed and unabsorbed elisas for the diagnosis of paratuberculosis in sheep. | a complement fixation test for paratuberculosis, a gel diffusion test and two enzyme-linked immunosorbent assays (elisa) were evaluated using sera from mycobacterium paratuberculosis infected and non-infected sheep. gross pathology and histopathology were used as parameters of infection. the two elisas, one of which is commercially available for testing cattle, were used before and after sera had been absorbed with a soluble sonicate of mycobacterium phlei. differences between the various tests ... | 1994 | 7801513 |
| polyamines exert regulatory control on mycobacterial transcription: a study using rna polymerase from mycobacterium phlei. | we have used purified rna polymerase from mycobacterium phlei to study the role of polyamines in mycobacterial transcription. both initiation and elongation phases of the process were affected biphasically by polyamines. interaction of polyamines with dna template plays an important role in transcription modulation. our studies emphasize that polyamines can exert a regulatory control on mycobacterial transcription. | 1995 | 7492956 |
| disinfection of dental stone casts: antimicrobial effects and physical property alterations. | the purpose of this study was to evaluate the effectiveness of disinfecting solutions incorporated into dental stone casts against a standard and representative group of microorganisms and to note changes in the physical properties of the casts. | 1995 | 7498603 |
| evaluation of an enzyme-linked immunosorbent assay licensed by the usda for use in cattle for diagnosis of ovine paratuberculosis. | a commercially available mycobacterium phlei-absorbed enzyme-linked immunosorbent assay (elisa) approved to detect antibodies to mycobacterium paratuberculosis in cattle was evaluated for its applicability in sheep. the potential for interference with elisa results from cross-reacting antibodies to corynebacterium pseudotuberculosis was also investigated. serum samples were randomly selected from a collection of samples obtained in 1986-1991 from 6 infected and 5 noninfected sheep flocks varying ... | 1995 | 7578450 |
| analysis of mycolic acids by high-performance liquid chromatography and fluorimetric detection. implications for the identification of mycobacteria in clinical samples. | mycolic acids from mycobacterium phlei and m. bovis cell wall skeletons (cwss) were analyzed by hplc. after saponifying lyophilized cwss in methanolic koh, the mycolic acids were quantitatively extracted into chloroform. aliquots of the cws mycolic acid extracts were then derivatized prior to hplc analysis with a uv reagent, p-bromophenacylbromide (pbpb), and three fluorescent reagents, 4-bromomethyl-6,7-dimethoxycoumarin, 4-bromomethyl-7-acetoxycoumarin and 3-bromomethyl-7-methoxy-1,4-benzoxazi ... | 1995 | 7719452 |
| ex vivo induction of tnf-alpha and il-6 mrna in bovine whole blood by mycobacterium paratuberculosis and mycobacterial cell wall components. | johne's disease is a chronic enteritis of cattle and other ruminant species that is of worldwide economic importance. the cytokines tumor necrosis factor-alpha (tnf-alpha) and interleukin-6 (il-6) have been associated with granuloma formation and wasting in other disease syndromes. the potential role of these cytokines in the development and progression of johne's disease has not been investigated. using reverse transcriptase polymerase chain reaction (rt-pcr) and specific bovine oligonucleotide ... | 1995 | 8559037 |
| antimicrobial action of the leaf extract of moringa oleifera lam. | the ethnolic extract of the leaves of moringa oleifera lam. (fam. moringaceae) was tested for antimicrobial activities against gram positive - bacillus cereus, bacillus subtilis staphylococcus aureus, sarcina lutea: gram negative - escherichia coli and acid fast mycobacterium phlei. significant antimicrobial activity of the extract was found in this study. | 1995 | 22556699 |
| attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
| characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probes | the structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ... | 1996 | 8688004 |
| mycobacterium cell wall: an alternative to intravesical bacillus calmette guerin (bcg) therapy in orthotopic murine bladder cancer. | the antitumor effect of intravesical mycobacterium cell wall (mcw) therapy on orthotopic and heterotopic bladder tumors in the mouse was assessed with magnetic resonance imaging (mri). | 1996 | 8709344 |
| chemistry of the lyxose-containing mycobacteriophage receptors of mycobacterium phlei/mycobacterium smegmatis. | mycobacterium phlei (strain timothy) (mycobacterium smegmatis atcc 19249) is characterized by the presence of a family of alkali-labile glycolipids, reminiscent of the trehalose-containing lipooligosaccharide class of antigens but lacking the nonreducing trehalose core. through a combination of methylation analyses, 1h and 13c nmr, two-dimensional 1h/1h and 1h/13c nmr, fast atom bombardment-mass spectrometry, gas chromatography-mass spectrometry, and other analytical techniques, these new struct ... | 1996 | 8794763 |
| paratuberculosis in small ruminants, deer, and south american camelids. | paratuberculosis in small ruminants is widespread geographically. in some herds and flocks, clinical paratuberculosis, which primarily causes long-term weight loss, can be a significant cause of culling. the effects of subclinical disease are less defined, but they may include decreased milk production in milking sheep and decreased weight gain in deer raised for slaughter. paratuberculosis also can cause economic losses due to reduced sales of breeding animals from purebred flocks and herds. di ... | 1996 | 8828115 |
| [taxonomic study of species of the mycobacterium genus isolated in cuba]. | 40 strains of the mycobacterium genus corresponding to 12 species, which were subjected to 62 microbiological and biochemical tests, were studied. each one was considered as a character. as a result of the similitude coefficient and their grouping, 9 phenomes represented by: phenome i (mycobacterium fortuitum), phenome ii (mai complex), phenome iii (mycobacterium phlei), phenome iv (mycobacterium triviale), phenome v (mycobacterium smegmatis), phenome vi (mycobacterium gordonae), phenome vii (my ... | 1996 | 9768276 |
| effect of mycobacterium phlei on the development of immunity to babesia bigemina. | the immunomodulatory role of mycobacterium phlei against intracellular blood protozoan babesia bigemina was demonstrated following experimental immunisation and challenge in bovine calves. a lysate of erythrocytes infected (6 x 10(9)) with b. bigemina was used as a source of dead antigen either with freund's complete adjuvant (fca) or with a trypsinised culture of m. phlei as a non-specific immunomodulation (nsi) agent with appropriate controls. following virulent challenge with b. bigemina infe ... | 1996 | 8686168 |
| effective adjuvants for the induction of antigen-specific delayed-type hypersensitivity. | vaccines utilizing poorly immunogenic subunit antigens are dependent upon adjuvants to drive the appropriate t cell responses. in an effort to determine the ability of several adjuvants to promote cell-mediated immunity (cmi), we assessed delayed-type hypersensitivity (dth) in mice inoculated with heat-killed listeria monocytogenes (hklm) vaccines. the vaccines were formulated as oil-in-water emulsions containing one or more of the following bacterial-derived immunostimulators: mpl immunostimula ... | 1997 | 9141210 |
| environmental mycobacteria in bottled table waters in greece. | a hundred and fifty samples of bottled table water sold by greek factories were examined for the presence of environmental mycobacteria. environmental mycobacteria were found in 23 (15.6%) of the 150 tested samples. bacterial numbers of 1-100, 101-300, 301-1000, and > 10(3) cfu/l were found in 8, 2, 1, and 4% of the samples, respectively. the identification of the environmental mycobacteria was performed by both polymerase chain reaction-restriction enzyme analysis (pcr-rea) and biochemical meth ... | 1997 | 9165705 |
| [toxic effect of hydroxylated ions of heavy metals on the cytoplasmic membrane of bacterial cells]. | the influence of nickel, copper, cadmium, and lead ions at concentrations of 50 to 100 microm on the barrier properties of the plasma membrane (pm) and the electrophoretic mobility (epm) of pseudomonas fluorescens 71, escherichia coli k-12, and mycobacterium phlei b-1291 vkm cells was studied at ph values from 5 to 9 by electro-orientational (eo) spectroscopy and microelectrophoresis of cells. according to the data of eo spectroscopy, the increase in the toxicity of heavy metal cations to cells ... | 1997 | 9424558 |
| mycobacterium phlei peritonitis: a rare complication of chronic peritoneal dialysis. | we report the first case of chronic ambulatory peritoneal dialysis-associated peritonitis caused by mycobacterium phlei. this organism was isolated from the peritoneal fluid of a patient who presented with recurrent episodes of "culture-negative" peritonitis. the therapeutic regimen was based on previous experience with other rapidly growing atypical mycobacteria, and included removal of the tenckhoff catheter, institution of hemodialysis, and anti-mycobacterial therapy with amikacin, cefoxitin, ... | 1998 | 9502573 |
| effect of immunostimulation on cytotoxic activity of intestinal intraepithelial lymphocytes of chickens in infectious bursal disease and eimeria tenella infections. | in chickens, intestinal intraepithelial lymphocytes (iiels) exhibit spontaneous natural killer (nk) cell like activity, by which they are active in the first line of defence on gut epithelial linings. in the present study, the cytotoxic activity of iiels was found to be drastically suppressed in chickens experimentally infected with infectious bursal disease (ibd) virus at the age of 5 weeks and also in chickens experimentally infected with eimeria tenella oocysts at the age of 8 weeks (p < 0.01 ... | 1998 | 9704505 |
| fusion of azurophil granules with phagosomes and activation of the tyrosine kinase hck are specifically inhibited during phagocytosis of mycobacteria by human neutrophils. | pathogenic mycobacteria parasitize macrophages and reside within phagosomes, which do not fuse with lysosomal granules. mycobacteria are also internalized by neutrophils, which possess at least two types of granules, specific and azurophil granules, the latter being specialized lysosomes. here, we investigated the ability of mycobacteria to inhibit the fusion of these granules with their phagosomes in human neutrophils. it was found that when pathogenic (mycobacterium kansasii and mycobacterium ... | 1998 | 9794435 |
| characterization of a haemolysin from mycobacterium tuberculosis with homology to a virulence factor of serpulina hyodysenteriae. | scrutiny of sequence data from the mycobacterium leprae genome sequencing project identified the presence of a gene encoding a 268-amino-acid polypeptide which is highly similar to a pore-forming haemolysin/cytotoxin virulence determinant, tlya, from the swine pathogen serpulina hyodysenteriae. using degenerate oligonucleotide primers based on the tlya sequences, the mycobacterium tuberculosis homologue was amplified and this product was used to obtain the clone and sequence a 2.5 kb fragment co ... | 1998 | 9611795 |
| isolation, purification and characterization of intracellular calmodulin like protein (calp) from mycobacterium phlei. | a monomeric acidic protein of 14,000 da with an isoelectric point of 4.5 was isolated from mycobacterium phlei, which stained poorly with coomassie brilliant blue. this protein showed retardation in mobility in sds-page upon treatment with calcium, similar to eukaryotic calmodulin proteins. activation of camp phosphodiesterase and nad kinase by this protein was observed. the cd spectral analysis indicated that the calp has 52% of beta-conformation. the regular beta-conformation of the calmodulin ... | 1998 | 9485591 |
| mycobacterium phlei cell wall complex directly induces apoptosis in human bladder cancer cells. | intact mycobacteria and mycobacterial cell wall extracts have been shown to inhibit the growth of human and murine bladder cancer. their mechanism of action is, however, poorly understood. mycobacterium phlei mycobacterial cell complex (mcc) is a cell wall preparation that has mycobacterial dna in the form of short oligonucleotides complexed on the cell wall surface. in this study, we have investigated the possibility that mcc has anti-cancer activity that is mediated by two different mechanisms ... | 1999 | 9888462 |
| partial characterization of a major autolysin from mycobacterium phlei. | autolytic enzyme profiles of fast- and slow-growing mycobacteria were examined using sds-page zymography with incorporated mycobacterial peptidoglycan sacculi as substrate. each species tested (mycobacterium phlei, mycobacterium smegmatis, mycobacterium aurum, mycobacterium fortuitum and mycobacterium kansasii) appeared to produce a different set of enzymes on the basis of differing number and molecular masses. a major autolysin from m. phlei was purified to apparent homogeneity by deae-cellulos ... | 1999 | 10206696 |
| active specific immunotherapy for metastatic colorectal carcinoma: phase i study of an allogeneic cell vaccine plus low-dose interleukin-1 alpha. | a vaccine consisting of four allogeneic colon carcinoma cell lines (dld-1, hct116, widr, and t84) mixed with the adjuvant detox (mycobacterium phlei cell wall and salmonella minnesota lipid a) was administered to 25 patients with low-volume metastatic colorectal carcinoma. the first eight patients received vaccine only, given intradermally on three occasions at 3-week intervals. subsequent patients also received subcutaneous interleukin-1 alpha (il-1 alpha), 0.3-0.5 microgram/m2 per day for 8 da ... | 1999 | 10335485 |
| serum antibodies to mycobacterium paratuberculosis in patients with crohn's disease. | mycobacterium paratuberculosis has been suggested as a causative organism of crohn's disease. despite a long-term debate to prove this possibility, the role of this bacteria in the pathogenesis of crohn's disease is still a subject of controversy. in the present study, serum antibodies (igg, iga, and igm) to the protoplasmic antigen of m. paratuberculosis were quantified in patients with crohn's disease and in control subjects by using an enzyme-linked immunosorbent assay whose specificity was i ... | 1999 | 10389697 |
| amplification of fluorescently labelled dna within gram-positive and acid-fast bacteria. | representative organisms from a variety of gram-positive genera were subjected to varying regimes in order to optimise the intracellular amplification of dna. the bacteria were subjected to treatments with paraformaldehyde, muramidases and mild acid hydrolysis to discover which regime made each organism permeable to the amplification reagents yet allowed retention of the fluorescein-labelled amplified products within the cell. scanning electron micrographs were used to corroborate the effectiven ... | 1999 | 10520585 |
| modulation of interleukin-12 synthesis by dna lacking the cpg motif and present in a mycobacterial cell wall complex. | a mycobacterial cell wall complex prepared from the non-pathogenic microorganism mycobacterium phlei, where mycobacterial dna is preserved and complexed to cell wall fragments, possesses anticancer and immunomodulatory activity. dna from a number of prokaryotes has been found to modulate the immune system and to induce cytokine synthesis. we have therefore determined whether the dna associated with this complex has the ability to induce the synthesis of interleukin-12 (il-12), a potent anticance ... | 2000 | 10946815 |
| phagocytosis of mycobacteria by u937 cells: a rapid method for monitoring uptake and separating phagocytosed and free bacteria by magnetic beads. | a human-derived monocytic cell line (u937) was induced to phagocytose mycobacterium phlei by the addition of phorbol myristate acetate (pma) to the culture medium for 50-60 h. cells not treated with pma were unable to phagocytose m. phlei. magnetic beads enabled a rapid and highly efficient separation of phagocytosed and free bacteria to be achieved, an approach which is particularly useful if colony plating is used to enumerate bacterial survival within phagocytic cells. fluorescence-activated ... | 2000 | 10728569 |
| antimicrobial activity of extracts of eastern north american hardwood trees and relation to traditional medicine. | wood and bark extracts of 14 eastern north american hardwood tree species which were used traditionally as medicine by first nation's people were screened for antimicrobial activities with eight strains of bacteria and six strains of fungi. eighty-six percent of the bark extracts were active against methicillin sensitive staphylococcus aureus; 71% against bacillus subtilus and 79% against mycobacterium phlei. the bark extract of juglans cinerea was active against pseudomonas aeruginosa 187, salm ... | 2000 | 11025152 |
| nonopsonic phagocytosis of zymosan and mycobacterium kansasii by cr3 (cd11b/cd18) involves distinct molecular determinants and is or is not coupled with nadph oxidase activation. | complement receptor type 3 (cr3) was initially described as an opsonic receptor. subsequently, cr3-mediated lectin-sugar recognition mechanisms have been shown to play a major role in the nonopsonic phagocytosis of several pathogens, among them mycobacterium tuberculosis. little is known about the binding and signal transduction mechanisms operating during nonopsonic ingestion through cr3 of different microorganisms. in the present study, we used cho cells stably transfected with cr3 to show tha ... | 2000 | 10899880 |
| mycobacterial cell wall extract for treatment of carcinoma in situ of the bladder. | bacillus calmette-guerin (bcg) established immunotherapy as an effective modality for carcinoma in situ of the bladder and remains the most effective agent for treatment. however, as a live organism it has the potential for undesirable side effects and toxicity. this result has led to the search for other active and safer biological response modifiers. we investigated the efficacy of a mycobacterial cell wall extract (mcwe) from mycobacterium phlei, which does not contain live bacteria, for mana ... | 2001 | 11586191 |
| characterization of biofilm growth and biocide susceptibility testing of mycobacterium phlei using the mbec assay system. | the importance of non-tuberculosis mycobacterial biofilm species in medicine, industry and the environment has recently gained attention. our objectives were to characterize biofilm growth of mycobacterium phlei m4, as a model of rapidly growing mycobacteria using the minimal biofilm eradication concentration (mbec) and to compare biocide susceptibility of planktonic and biofilm organisms. scanning electron microscopy was also carried out to observe biofilm morphology. with the exception of spor ... | 2001 | 11583858 |
| thermophilic biodesulfurization of dibenzothiophene and its derivatives by mycobacterium phlei wu-f1. | dibenzothiophene (dbt) derivatives can be detected in diesel oil following hydrodesulfurization treatment, and they are widely recognized as target compounds for more efficient desulfurization. the moderately thermophilic bacterium mycobacterium phlei wu-f1 was isolated for its ability to grow at 50 degrees c in a medium with dbt as the sole source of sulfur. at 50 degrees c, resting cells of wu-f1 degraded 0.81 mm dbt within only 90 min to produce 2-hydroxybiphenyl as a desulfurized metabolite ... | 2001 | 11682191 |