Publications

psammoma bodies of benign endometrial origin in cervicovaginal cytology.	this case report illustrates a benign endometrial condition associated with a combination oral contraceptive agent in which psammoma bodies were produced. additionally, it reemphasizes the fact that nonmalignant processes are capable of forming such structures.	1990	269607
[a private dental laboratory must not work for outside dentists].		1990	269589
[physiology and pathophysiology of antibacterial phagocytosis. i. physiology].		1990	171544
evaluation of restriction endonuclease fingerprinting of chromosomal dna and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates.	a procedure was developed for restriction endonuclease fingerprinting (ref) of the chromosomal dna of coagulase-negative staphylococci. a total of 48 isolates comprising 29 staphylococcus epidermidis and 19 staphylococcus haemolyticus isolates from blood and mucocutaneous sites of 15 premature neonates were characterized by ref, plasmid profile (pp) analysis, antimicrobial susceptibility testing, biotyping, and slime production. on the basis of ref analysis of chromosomal dna, the 48 coagulase-n ...	1990	1968906
homology of meca gene in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans to that of staphylococcus aureus.	a penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant staphylococcus haemolyticus and staphylococcus simulans. dna from these strains hybridized to the meca gene from staphylococcus aureus; however, the chromosomal hindiii fragments containing the meca genes were 3.4 kilobases in s. haemolyticus and 4.3 kilobases in s. simulans.	1990	1691614
comparative study of hemolytic substances produced by coagulase-negative staphylococcus strains.	hemolytic substances h7, h62, and e56, produced by staphylococcus haemolyticus 7 and 62 and s. epidermidis 56, respectively, were purified. h7 and h62 are probably similar on the basis of their isoelectric focusing profiles in 8 m urea and complete immunological identity as revealed by immunodiffusion with rabbit anti-h7 and anti-e56 sera. for e56, we observed seven bands instead of three in isoelectric focusing and only partial immunological identity with h7 and h62. however, h7 and e56 were si ...	1990	2114358
failure of teicoplanin therapy in two neutropenic patients with staphylococcal septicemia who recovered after administration of vancomycin.	a report is given on two neutropenic patients with staphylococcal septicemia caused by staphylococcus haemolyticus and staphylococcus aureus (both strains methicillin-resistant) who failed to respond to therapy with teicoplanin. both strains were resistant to teicoplanin (mic 16 and 8 mg/l respectively), but remained sensitive to vancomycin (mic 2 and 4 mg/l respectively). replacement of teicoplanin with vancomycin led to full recovery of both patients and their discharge from hospital. these tw ...	1990	2138543
selection of vancomycin- and teicoplanin-resistant staphylococcus haemolyticus during teicoplanin treatment of s. epidermidis infection.		1990	2140112
phage-typing of coagulase-negative staphylococci. factors influencing typability.	factors influencing the phage-typability of coagulase-negative micrococcaceae have been studied in 2,778 clinical isolates comprising a) 209 consecutive isolates from one laboratory, b) 2,107 clinical strains submitted for phage-typing for epidemiological reasons, and c) 462 strains representing all isolates of presumed clinical significance found in two laboratories during one month. the reproducibility was acceptable at duplicate repeated typing of the same strains as well as by typing epidemi ...	1990	2141261
[the use of teicoplanin in neutropenic patients: values and limits].	the efficacy and the safety of teicoplanin were evaluated for the treatment of febrile episodes in neutropenic patients. a total of 18 patients received teicoplanin once daily as an intravenous injection of 200 mg in 6 patients (after a loading dose of 400 mg) or 400 mg in 12 patients (loading dose: 800 mg). the mean duration of therapy was 15.9 days (range 9 to 39 days). in all patients teicoplanin was combined with another antibiotic usually a beta-lactam. thirteen of 18 patients were successf ...	1990	2143573
in vitro activity of ly264826, a new glycopeptide antibiotic, against gram-positive bacteria isolated from patients with cancer.	the in vitro activity of ly264826, a novel glycopeptide antibiotic produced by amycolatopsis orientalis, was compared with those of vancomycin, teicoplanin, and oxacillin against 311 gram-positive clinical isolates from patients with cancer, ly264826 had lower mics for 90% of isolates (mic90) than vancomycin for all species tested. it was active against oxacillin-resistant isolates including staphylococcus aureus (mic90, 0.5 micrograms/ml), staphylococcus haemolyticus (mic90, 2.0 micrograms/ml), ...	1990	2149921
sulfation of tyrosine residues in coagulation factor v.	sulfation of human coagulation factor v was investigated by biosynthetically labeling the products of hepg2 cells with [35s]sulfate. there was abundant incorporation of the sulfate label into a product identified as factor v by immunoprecipitation, lability to proteases, affinity for the lectin jacalin, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. two or more sites in factor v incorporated sulfate as indicated by labeling of different peptide chains of factor va. the 150-kd act ...	1990	2168225
collagens synthesized by healing fractures.	several extracellular matrices are formed by healing fractures, and the collagens within these matrices have been identified in rabbit and rat fracture models. type iii collagen is the major collagen of the fibrous matrix that forms along the periosteal surface. type i collagen is secreted in large amounts as trabeculae of bone develop within the fibrous tissue. type v collagen is found in both the fibrous tissue and bone; it is particularly associated with blood vessels. type ii collagen is the ...	1990	2189630
ventilatory management of ards: can it affect the outcome?	animal studies have demonstrated that mechanical ventilation with high peak inspiratory pressure (pip) results in acute lung injury characterised by hyaline membranes, granulocyte infiltration and increased pulmonary and systemic vascular permeability. this can result in progressive respiratory failure and death. in surfactant deficient lungs this occurs with tidal volumes (vt) as low as 12 ml/kg, and pip as low as 25 cm h2o, values which are frequently used clinically. the mechanisms resulting ...	1990	2193041
characterization of the cdna coding for mouse prothrombin and localization of the gene on mouse chromosome 2.	a series of overlapping cdnas coding for mouse prothrombin (coagulation factor ii) have been isolated and the composite dna sequence has been determined. the complete prothrombin cdna is 1,987 bp in length [excluding the poly(a) tail] and codes for 18 bp of 5' untranslated sequence, an open reading frame coding for 618 amino acids, a stop codon, and a 3' untranslated region of 112 bp followed by a poly(a) tail. the translated amino acid sequence predicts a molecular weight of 66,087, which inclu ...	1990	2222810
trends in canadian tobacco consumption, 1980-89.		1990	2224718
trends in canadian tobacco consumption, 1980-89.		1990	2224718
comparative in vitro activity of cefpirome and cefepime, two new cephalosporins.	in in vitro tests the broad-spectrum cephalosporins cefpirome and cefepime were highly active against enterobacteriaceae, although often less so against strains resistant to amoxicillin-clavulanate and ticarcillin-clavulanate, and against most strains of acinetobacter spp. and aeromonas hydrophila. they were also active against pseudomonas aeruginosa, although strains with non-plasmid mediated beta-lactam resistance were sometimes less sensitive. other pseudomonas spp. varied in their sensitivit ...	1990	2226498
vancomycin resistance in staphylococcus haemolyticus causing colonization and bloodstream infection.	the increase in the incidence of infections due to beta-lactam-resistant coagulase-negative staphylococci has resulted in expanded use of vancomycin for such infections. despite this, coagulase-negative staphylococci have remained susceptible to vancomycin in recent years. this report describes a strain of staphylococcus haemolyticus with increased resistance to vancomycin (mic, 8.0 to 16 micrograms/ml). s. haemolyticus was initially isolated from a patient with acute leukemia and neutropenia in ...	1990	2229388
neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats.	intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ...	1990	2245800
neonatal monosodium glutamate abolishes corticotropin-releasing factor-induced epileptogenic activity in rats.	intracerebroventricular (i.c.v.) injection of rat corticotropin-releasing factor (rcrf) at doses of 5-20 micrograms in rats induces epileptogenic activity characterized by pacemaker-like spikes localized in the hippocampal leads. such an effect was still present in rats neonatally treated with saline but was absent in those neonatally treated with monosodium glutamate (msg), a treatment that caused marked changes in the concentration of several brain neurotransmitters and neuropeptides in hypoth ...	1990	2245800
tardive eating dystonia.	after cessation of long-term treatment with haloperidol, a 77-year-old man developed severe dyskinetic-dystonic movements involving mainly the tongue and associated also with oromandibular dystonia and blepharospasm that were manifested exclusively during the process of eating and that interfered with feeding. there were no spontaneous involuntary movements nor were they apparent during any other simple or complex activations of the orofacial musculature.	1990	2259358
presence of an additional penicillin-binding protein in methicillin-resistant staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus hominis, and staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole.	the presence of an additional penicillin-binding protein (pbp) was demonstrated in methicillin-resistant strains of staphylococcus epidermidis, s. haemolyticus, s. hominis, and s. simulans. in these four species, the apparent molecular mass of this protein was analogous to that of pbp 2' of methicillin-resistant s. aureus sr 1550-9. it exhibited a low affinity for methicillin, cephalothin, and cefamandole; and its synthesis was methicillin inducible. peptide mapping of this pbp from the four spe ...	1990	2285281
mathematical modeling of antimicrobial susceptibility data of staphylococcus haemolyticus for 11 antimicrobial agents, including three experimental glycopeptides and an experimental lipoglycopeptide.	antimicrobial mic data were obtained for 96 strains of staphylococcus haemolyticus and the following 11 antimicrobial agents: methicillin, gentamicin, rifampin, fusidic acid, ciprofloxacin, vancomycin, teicoplanin; three experimental glycopeptides, mdl 62,873, mdl 62,208, and mdl 62,224; and an experimental lipoglycopeptide, ramoplanin. resistance to methicillin and gentamicin was present in over 50% of the strains, although resistance to the other agents was present in less than 10%. it is show ...	1990	2285289
fulminant falciparum malaria.	a case of fulminant falciparum malaria with a 35% parasitaemia, shock and subcoma was treated successfully by using parenteral chemotherapy, exchange transfusion, dexamethasone, circulatory support and mechanical ventilation. pathophysiology and complications of falciparum malaria are discussed. the treatment of severe malaria should aim for a fast reduction in parasitaemia and toxic products. an exchange transfusion can be additive to parenteral chemotherapy. blocking the over-reacting cell-med ...	1990	2286734
selection for vancomycin resistance in clinical isolates of staphylococcus haemolyticus.	killing curves were used to characterize staphylococcus haemolyticus isolates previously reported to contain subpopulations showing increased resistance to vancomycin. results suggested that vancomycin and teicoplanin were ineffective at a concentration of 8 micrograms/ml and growth was seen between 24 and 48 h. conversely, the lipopeptide antibiotic daptomycin at the same concentration rapidly killed tested strains by 6 h. various staphylococcal strains were examined to determine if vancomycin ...	1990	2295858
reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to staphylococcus aureus capsular polysaccharide types 5 and 8.	monoclonal antibodies to staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. eighteen (15.5%) isolates were typable: 13 staphylococcus haemolyticus, 1 s. hyicus, 1 s. simulans, and 1 s. warneri from bovine origin and 2 s. lentus from caprine origin. type 5 was predominant, accounting for about 89% of typable isolates. reactivity with monoclonal antibod ...	1990	2312680
peripheral teflon catheters. potential source for bacterial contamination of orthopedic implants?	to detect low numbers of bacterial cells on teflon catheters removed from peripheral veins, 74 catheter tips were incubated in thioglycollate media for up to ten days. fifteen (20.3%) of the catheters were found to be culture-positive. propionibacterium acnes was isolated from seven, staphylococcus epidermidis from six, and staphylococcus haemolyticus and staphylococcus hominis from one each, all common skin organisms associated with infection of implants. an increase in documentation of bacteri ...	1990	2323139
staphylococcal enterotoxins direct and trigger ctl killing of autologous hla-dr+ mononuclear leukocytes and freshly prepared leukemia cells.	attempts have been made to induce cytolytic t cells to kill target cells that do not express the appropriate target molecules by crosslinking the t cells and the target cells in various ways. one successful strategy has been to use heteroconjugates or bispecific monoclonal antibodies reacting with t cell molecules with activating properties (e.g., mab directed to cd3/tcr) and target cell surface antigens. in this report we show that staphylococcal enterotoxins (se) direct human t lymphocytes to ...	1990	2383900
[empirical treatment of febrile episodes in granulocytopenic patients with a combination of piperacillin and ofloxacin. preliminary study].	we evaluated the efficacy and toxicity of piperacillin-ofloxacin as an empiric treatment of fever in patients with neutropenia. 24 febrile episodes occurring in 21 patients (mean neutropenia: 204/mm3) were treated. the neutropenia was due to an hematologic malignancy in 6 cases and to chemotherapy in 15 cases. fever was related to septicemia in 4 cases, urinary tract infection in 1 case, other infectious sites without microbiological documentation in 11 cases, and was of unknown origin in 8 case ...	1990	2385453
haemophilus influenzae bartholinitis.		1990	2387300
effects of retrograde flow on measured blood volume, disse space, intracellular water space and drug extraction in the perfused rat liver: characterization by the multiple indicator dilution technique.	previous rat liver perfusion studies had shown a preferential derecruitment of downstream (phenacetin o-de-ethylation) in relation to upstream (acetaminophen sulfation) hepatocyte activities with reductions in prograde (normal) flow, with substrates entering from the portal vein and exiting the hepatic vein. the reduction in metabolic activities was found associated with a reduction in accessible intracellular water space (pang et al., j. pharmacokinet. biopharm. 16: 595, 1988). in the present s ...	1990	2395120
detection of cytomegalovirus in lung allografts. comparison of histologic and immunohistochemical findings.	although the histologic manifestation of cytomegalovirus (cmv) is usually characteristic intracellular inclusions and cytomegaly, some investigators, using immunohistochemical or in situ hybridization techniques, have demonstrated the presence of histologically occult infections in certain tissues. a series of lung biopsy specimens from pulmonary transplant recipients were studied using a monoclonal antibody (cch2) to cmv early viral antigen and the results were compared with routine histologic ...	1991	1720923
detection of a soluble acrosome reaction-inducing factor, different from serum albumin, associated with the ovulated egg-cumulus complex.	soluble extracts of the ovulated hamster egg-cumulus complex (ecc) were tested on capacitated sperm for activity in inducing the physiological acrosome reaction (ar). evidence for occurrence of the physiological ar included enhanced sperm penetration of intact homologous zonae pellucidae as well as induction of ar in nonattached and in zona-bound sperm following a brief coincubation with test compound. since hamster serum albumin, a major protein of hamster body fluids, also induces spontaneous ...	1991	1751045
susceptibility of staphylococcus species and subspecies to fleroxacin.	twenty-four staphylococcus species or subspecies were examined for their susceptibilities to the fluoroquinolone fleroxacin (ro 23-6240) by disk diffusion (5-micrograms disk) and by agar dilution for the determination of mics. resistant strains were further tested for their susceptibilities to oxacillin and the fluoroquinolone ciprofloxacin. reference strains of the novobiocin-resistant species (staphylococcus saprophyticus, staphylococcus cohnii, staphylococcus xylosus, staphylococcus arlettae, ...	1991	1759838
repletion and depletion of vitamin e in gastroduodenal mucosa.	male rats were used to assess the effect of fasting and depletion and repletion rates on the levels of vitamin e in the liver and in mucosa from the fundus, antrum, and duodenum. fasting for 24 h did not produce a significant decrease in vitamin e content in gastroduodenal mucosa. feeding rats a diet low in vitamin e content for 10 weeks produced very low levels of the vitamin in all the tissues studied (below 2.4 micrograms/g tissue). feeding rats a diet high in vitamin e content for 10 weeks p ...	1991	1771388
in vitro selection of resistance to vancomycin in bloodstream isolates of staphylococcus haemolyticus and staphylococcus epidermidis.	the purpose of the study was to determine whether vancomycin-resistant strains of staphylococcus haemolyticus could be selected regardless of the initial mic of vancomycin. twenty-one bloodstream isolates of staphylococcus haemolyticus were studied by broth and agar selection methods. the broth method selected strains for which mics of vancomycin ranged from 4 to 32 micrograms/ml and mbcs from 16 to greater than 128 micrograms/ml. the agar method selected strains for which mics ranged from 8 to ...	1991	1802693
comparative in vitro activity of teicoplanin and vancomycin against united states teicoplanin clinical trial isolates of gram-positive cocci.	in this study, the in vitro activity of teicoplanin and vancomycin was directly compared against 503 gram-positive cocci isolated during the u.s. teicoplanin clinical trials. both antibiotics were equally active against oxacillin-sensitive staphylococcus aureus, oxacillin-sensitive and oxacillin-resistant staphylococcus epidermidis, and other coagulase-negative staphylococci, except staphylococcus haemolyticus. teicoplanin was fourfold more active than vancomycin against oxacillin-resistant s. a ...	1991	1826479
activities of two new teicoplanin amide derivatives (mdl 62211 and mdl 62873) compared with activities of teicoplanin and vancomycin against 800 recent staphylococcal isolates from france and the united states.	mdl 62211 is the amide derivative of the teicoplanin complex and mdl 62873 is a more focused amide derivative of the teicoplanin a2-2 peak. each investigational compound had nearly identical activity and was 2- to 16-fold more active than teicoplanin or vancomycin. the mdl 62873 mics for 90% of the strains tested were as follows: staphylococcus aureus, oxacillin susceptible, 0.12 micrograms/ml; s. aureus, oxacillin resistant, 0.25 micrograms/ml; coagulase-negative staphylococci (cns), oxacillin ...	1991	1828137
susceptibility of staphylococcus species and subspecies to teicoplanin.	twenty-four staphylococcus species and their subspecies were examined for their susceptibilities to teicoplanin by disk diffusion (30-micrograms disk) and agar dilution for the determination of mics. moderately susceptible and resistant clinical strains were further tested for their susceptibilities to oxacillin and vancomycin. teicoplanin resistance was not observed in the reference strains of the various staphylococcus species isolated from healthy volunteers or animals. however, the novobioci ...	1991	1835340
comparative therapeutic efficacy of teicoplanin and vancomycin in normal and in neutropenic mice infected with staphylococcus haemolyticus.	this study describes the evaluation of a murine bacteraemia model for assessing antibiotic efficacy in normal and neutropenic mice infected with coagulase-negative staphylococci. in one such evaluation, it was found that there was no significant (p greater than 0.05) difference in the ability of teicoplanin or vancomycin to protect normal or neutropenic cd-1 mice, lethally-infected with staphylococcus haemolyticus. however, about a four-fold increase of either antibiotic was needed to protect th ...	1991	1838105
presence and localization of a 30-kda basic fibroblast growth factor-like protein in rodent testes.	we have used a recently characterized rabbit antiserum against basic fibroblast growth factor (bfgf), which recognizes various forms of bfgf, to examine the presence and localization of bfgf in the testes of adult rats and mice and the 5-day-old rat. in western blots of testicular homogenates of adult rats and mice and immature rats, immunoreactive single bands at approximately 30 kda were detected. immunocytochemistry revealed specific staining restricted to the tubular compartment. in 5-day-ol ...	1991	1855483
optimal conditions for long-term photoreceptor cell rescue in rcs rats: the necessity for healthy rpe transplants.	retinal pigment epithelial (rpe) cells from normal long-evans, healthy royal college of surgeons (rcs) pigmented congenic (rdy+p+) and rcs pigmented dystrophic rats were transplanted into retinas of rcs non-pigmented dystrophic rats at post-natal days 17 and 26 (p17 and p26). when examined at p60, rescued photoreceptor cells were found in all transplanted groups. in addition, a small, but significant increase in the outer nuclear layer (onl) thickness was detected in retinas injected with vehicl ...	1991	1855542
bacterial formation of n-nitroso compounds in the rat stomach after omeprazole-induced achlorhydria.	n-nitrosamine formation by bacteria in the achlorhydric stomach has been proposed as an important factor in the development of gastric cancer. thus, the effect of the presence of bacteria in the stomach on endogenous nitrosation was investigated in rats given omeprazole (an inhibitor of gastric h+, k((+)-atpase) which reduces gastric secretion sufficiently to allow survival of a bacterial suspension of escherichia coli or pseudomonas. when rats were given both thiazolidine 4-carboxylic acid and ...	1991	1855848
infectious crystalline keratopathy caused by candida albicans.	two patients developed corneal opacities resembling infectious crystalline keratopathy. predisposing factors included a recent corneal transplant with suture replacement in one patient and postradiation keratoconjunctivitis with disposable therapeutic contact-lens wear in the other patient. both patients were using a topically applied corticosteroid and an aminoglycoside antimicrobial. smears of corneal scrapings showed numerous yeasts without inflammatory cells. culturing yielded candida albica ...	1991	1882943
antimicrobial activity of cefpirome. an update compared to five third-generation cephalosporins against nearly 6000 recent clinical isolates from five medical centers.	cefpirome, cefotaxime, ceftazidime, cefoperazone, ceftizoxime, and ceftriaxone were tested against approximately 6000 fresh clinical isolates from five medical centers. for 3031 strains of enterobacteriaceae tested, cefpirome consistently had the lowest mic50s and lowest percentage of resistant strains. cefpirome was also the most active agent against the 2138 gram-positive cocci tested; staphylococcus haemolyticus was uniformly resistant to all agents tested. against 791 nonenteric gram-negativ ...	1991	1889187
relative quantification of collagen mrna in fibroblasts by a radioactive polymerase chain reaction technique.	a radioactive polymerase chain reaction (pcr) method has been developed for the relative quantification of the human alpha-2 chain of type i collagen [hu alpha-2(i)] in cells. cdnas generated by reverse transcription from the total pool of cytoplasmic rna serve as a template for polymerase chain reaction amplification of a hu alpha-2(i) cdna primed by two sequence-specific synthetic oligonucleotides. the distinctive 390 bp hu alpha-2(i) cdna and two aval fragments of 220 and 170 bp are identifie ...	1991	1890540
submicromolar manganese dependence of golgi vesicular galactosyltransferase (lactose synthetase).	1. manganese(ii) buffers were set up with inorganic triphosphate, trimethylenediaminetetraacetate and tetramethylenediaminetetraacetate to study the mn dependence of beta 1,4-galactosyltransferase (lactose synthetase) in preparations of rat mammary gland. 2. in intact particulate preparations, treated with the calcium ionophore a23187, lactose synthesis was abolished by chelators and restored by bivalent transition metal ions in a manner characteristic of activation site i of the pure enzyme. ni ...	1991	1899383
molecular cloning, purification, and properties of a plasmid-encoded chloramphenicol acetyltransferase from staphylococcus haemolyticus.	a small chloramphenicol resistance (cmr) plasmid of approximately 3.75 kb, designated pscs5, was isolated from staphylococcus haemolyticus. this plasmid encoded an inducible chloramphenicol acetyltransferase (cat; ec 2.3.1.28). the cat gene of pscs5 was cloned into the escherichia coli plasmid vector pbluescript skii+. it differed in its nucleotide sequence and deduced amino acid sequence from the cat genes described previously in staphylococci and other gram-positive bacteria. the cat enzyme wa ...	1991	1929282
nucleotide sequence and phylogeny of a chloramphenicol acetyltransferase encoded by the plasmid pscs7 from staphylococcus aureus.	the nucleotide sequence of the chloramphenicol acetyltransferase gene (cat) and its regulatory region, encoded by the plasmid pscs7 from staphylococcus aureus, was determined. the structural cat gene encoded a protein of 209 amino acids, which represented one monomer of the enzyme chloramphenicol acetyltransferase (cat). comparisons between the amino acid sequences of the pscs7-encoded cat from s. aureus and the previously sequenced cat variants from s. aureus, staphylococcus intermedius, staphy ...	1991	1929326
enhanced healing of 30 microns gore-tex ptfe microarterial prostheses by alcohol-pretreatment.	polytetrafluoroethylene (ptfe) microvascular prostheses with a fibril length of 30 microns were pretreated with alcohol (n = 18), implanted into the abdominal aorta of rats and were evaluated at 1 day (n = 3), 1 week (n = 3), 3 weeks (n = 6) and 6 weeks (n = 6) to determine whether alcohol-pretreatment might improve their healing. untreated ptfe microvascular prostheses (n = 18) functioned as controls (all prostheses: length 10 mm, i.d. 1.5 mm). the alcohol-pretreated ptfe microarterial prosthes ...	1991	1933114
identification of methicillin-resistant strains of staphylococci by polymerase chain reaction.	a simple and reliable method using a polymerase chain reaction (pcr) was devised to identify methicillin-resistant staphylococci. by using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of meca, the structural gene of a low-affinity penicillin-binding protein (pbp 2'), was amplified by pcr and detected by agarose gel electrophoresis. results obtained by this method were compared with those obtained by broth microdilution mic determination ...	1991	1939577
neither heparin nor acetylsalicylic acid influence the clinical course in human plasmodium falciparum malaria: a prospective randomized study.	procoagulant alterations and thrombocytopenia in falciparum malaria correlate with parasitemia, serum levels of tumor necrosis factor alpha (tnf alpha), and clinical severity. thus, heparin or acetylsalicylic acid (asa), which are used frequently to prevent thrombosis and (in the case of asa) to control fever, could be potentially beneficial. we randomized 97 patients with falciparum malaria into three groups: 33 patients received low-dose heparin subcutaneously, 31 received asa intravenously, a ...	1991	1951871
the effect of prophylaxis with chloroquine and proguanil on delayed-type hypersensitivity and antibody production following vaccination with diphtheria, tetanus, polio, and pneumococcal vaccines.	in vitro studies have shown that anti-malarial drugs suppress immunity. in this study, the effects of chloroquine and proguanil (paludrine) on the cellular and humoral immune system were measured by two in vivo methods: 1) cell-mediated immunity (delayed cutaneous hypersensitivity) i.e., skin tests with seven delayed-type common antigens (multitest) and 2) humoral immunity by measurement of specific antibody response to vaccination. sixty healthy young individuals were randomized into four group ...	1991	1951872
evaluation of the staph-zym system with staphylococci isolated from bovine intramammary infections.	a total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the staph-zym system (rosco, taastrup, denmark). the overall accuracy of the system was 91.9%. the system correctly identified all strains of staphylococcus aureus, staphylococcus simulans, and staphylococcus xylosus and 95% of staphylococcus intermedius strains. of 33 staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the staph-zym system, as well as 8 (80%) of 10 staphylococcus c ...	1991	1993769
epidemiology of campylobacter enteritis.	campylobacter enteritis is the commonest form of infective diarrhoea in most developed countries of the world. in england and wales laboratory reports indicate an annual incidence of about 85/100,000, but the true rate is probably nearer 1100/100,000. measured costs run to many millions of pounds per year. most infections are sporadic and believed to be foodborne; large outbreaks are infrequent and mostly due to the consumption of raw milk or unchlorinated water. raw meats and animal products, n ...	1991	2018709
occurrence of staphylococcus lugdunensis in consecutive clinical cultures and relationship of isolation to infection.	consecutive record review over a 63-month period revealed 229 staphylococcus lugdunensis isolates, or 10.1% of the staphylococcal species that were not staphylococcus aureus or staphylococcus epidermidis. a total of 155 s. lugdunensis specimens were isolated from sites over the entire bodies of the 143 patients studied. the most common clinical diagnoses were skin and skin structure infections (55.4%) and blood and vascular catheter infections (17.4%). for 40% of the reviewed specimens, s. lugdu ...	1991	2037657
a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands.	the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ...	1991	2045549
molecular epidemiology of staphylococcus haemolyticus strains isolated in an albanian hospital.	a recent outbreak of erythroderma in young children in an albanian hospital was investigated. the etiology was not established, but staphylococcus haemolyticus was frequently isolated from the affected children and from staff working in the same unit. possible relationships among the isolates were investigated by using classical techniques (biotype, antimicrobial susceptibility, and extrachromosomal dna pattern) and by restriction endonuclease analysis (rea) of total dna. control isolates of pro ...	1991	1653266
observations on coagulase-negative staphylococci in a neonatal unit in india.	fifty neonates were included in a prospective study which determined the frequency of colonization and infection with coagulase-negative staphylococci (cns) in a neonatal unit. the isolates were characterised by species, antibiotic resistance and ability to produce slime. twenty-four epidemiologically related isolates (including four isolates from the hands of staff) were also phage typed and had plasmid analysis performed. on the first day of birth, 18% of infants were colonized with cns, 40% a ...	1991	1686040
veterinarian versus phone company.		1991	245217
[reorganized patient groups has cost something but is was worth it].		1991	245158
[developments in finnish occupational therapy following foreign models].		1991	245056
the catheter team: an essential service for rehabilitating neurogenic bladders.	a carefully controlled programme of intermittent catheterization provides substantially better care of the bladder than indwelling catheters during the initial period after acute spinal cord injury. it is very difficult to maintain a programme of intermittent catheterization around the clock without a small team of nursing personnel highly skilled in the technique of aseptic catheterization. the practical details of setting up a catheter team are described. some of the results that have been ass ...	1991	269703
[congenital dislocation of the hip].		1991	245341
parkinson's disease.		1991	245293
[changes in the masticatory system in a case of progressive myositis ossificans].		1991	270140
[odontomas with special reference to gardner's syndrome].	analysis of world literature appears to show that large hard odontomata of the jaw are relatively rare. 43 such odontogenous tumours are reported which were treated during 30 years in the maxillo-facial clinic in heidelberg and in which the feasibility of mandibular reconstruction following partial resection was considered. the attention of dentists and maxillo-facial surgeons is drawn to odontomata, which are part of the symptom complex of gardner's syndrome; a case is described where this rare ...	1991	269791
detection and purification of a potential precursor protein or a prohaemolysin produced by staphylococcus haemolyticus.	the haemolytic substance h7 produced by staphylococcus haemolyticus is composed of three peptides out of 44 amino acid residues each having a structure resembling a signal peptide. the cytoplasmic fraction for the presence of a protein precursor containing this signal sequence was investigated. specific rabbit iggs to substance h7 were isolated by affinity chromatography on sepharose-4b-h7. these anti-h7 iggs recognized mainly a 51 kd protein in the cytoplasmic fraction of s. haemolyticus from 2 ...	1992	1282650
novel membrane proteins present in teicoplanin-resistant, vancomycin-sensitive, coagulase-negative staphylococcus spp.	clinical isolates of staphylococcus epidermidis and staphylococcus haemolyticus resistant to teicoplanin (mic 64 mg/l) and sensitive to vancomycin (mic 2 mg/l), were compared with vancomycin- and teicoplanin-sensitive isolates (mics 1 mg/l) of the same species. no apparent differences between the sensitive and resistant strains of either pair were found with respect to binding of teicoplanin to the bacteria, or to the amino acid content or degree of cross-linkage of purified peptidoglycan. the r ...	1992	1289350
effects of gene transfer into cultured cns neurons with a replication-defective herpes simplex virus type 1 vector.	vectors derived from herpes simplex virus type 1 (hsv-1) may provide useful tools for gene transfer to cells of the mammalian nervous system. we have studied the infection of cultured cns neurons using a vector derived from an hsv-1 mutant deleted for the major hsv-1 transcriptional regulatory protein-encoding gene, ie 3. this vector, denoted cgal delta 3, contains the e. coli lacz gene driven by the strong promotor of the human cytomegalovirus major immediate-early gene inserted into a non-codi ...	1992	1312210
underdiagnosis of genital herpes by current clinical and viral-isolation procedures.	the current clinical strategy for diagnosing genital herpes simplex virus (hsv) infection in women relies on clinical findings plus the selective use of viral culture. the effectiveness of this approach for identifying women with genital herpes is unknown.	1992	1315930
metabolic inhibition and electrical properties of type-1-like cortical astrocytes.	type-1-like cortical mouse astrocytes were studied in homogeneous cultures. membrane input resistance and membrane potential were measured during drug-induced inhibition of glycolysis (sodium fluoride), mitochondrial respiration (antimycin-a) and na+/k+ pump activity (ouabain). it was found that the electrical properties of the astrocytes recovered after a 60 min period with inhibited glycolysis or mitochondrial respiration, exhibiting only small reversible depolarizations. a 60 min period of hi ...	1992	1315934
differential expression of two sodium channel subtypes in human brain.	two partial human brain sodium channel cdna sequences (designated hbsc i and ii) have been cloned and mapped to chromosome 2q23-2q24 by chromosome microdissection-pcr (cmpcr). the distribution of hbsc i and ii mrna in human brain was studied by means of a novel approach based on the ligase detection reaction. these studies demonstrate that hbsc i and ii mrna is heterogeneously distributed in brain, and that the relative ratio of the two forms can vary as much as 7-fold between different regions.	1992	1317301
in vitro evaluation of e-4695, a new fluoro-naphthyridine.	compound e-4695, a c-7 azetidinyl fluoro-naphthyridine, was compared to six structurally related quinolones and was generally two- to four-fold more active than ciprofloxacin. e-4695 was particularly active against staphylococcus aureus (mic90 0.06 mg/l), staphylococcus haemolyticus (mic90 0.5 mg/l), klebsiella spp. (mic90 less than or equal to 0.015 mg/l), serratia marcescens (mic90 0.06 mg/l), acinetobacter spp. (mic90 less than or equal to 0.015 mg/l), pseudomonas aeruginosa (mic90 0.5 mg/l), ...	1992	1327787
colonization with coagulase-negative staphylococci in two neonatal units.	episodes of septicaemia due to coagulase-negative staphylococci (cns) were more frequent in a level iii than in a level ii neonatal unit in stockholm, sweden. colonization with cns during the first 2 weeks of life was investigated in 10 infants from each unit. as the use of antibiotics differed between the two units, the aim was to correlate colonization and antimicrobial resistance patterns to antibiotic usage. antimicrobial susceptibility of cns to isoxazolylpenicillins, co-trimoxazole, erythr ...	1992	1363108
plasmid-encoded resistance to tetracycline in staphylococcus haemolyticus.	a small 4.35 kb plasmid, designated psts4, was isolated from a multiresistant staphylococcus haemolyticus culture. it conferred resistance to tetracycline as shown by protoplast transformation. psts4 was further characterized by restriction endonuclease analyses and a preliminary restriction map constructed. it shared some structural similarities with previously described small tcr plasmids from other staphylococcal species of human and animal origin. psts4 encoded two proteins of approximately ...	1992	1368787
rapid identification of fibronectin, vitronectin, laminin, and collagen cell surface binding proteins on coagulase-negative staphylococci by particle agglutination assays.	seventeen strains of ten different species of coagulase-negative staphylococci were shown to interact with collagen, laminin, fibronectin, and vitronectin immobilized on latex beads. different species of coagulase-negative staphylococci have different capacities to agglutinate proteins. cells of 18 strains of staphylococcus haemolyticus reacted more strongly than did cells of 18 staphylococcus epidermidis strains with proteincoated latex beads, although no significant difference in cell surface ...	1992	1380008
conformational changes in phospholipase a2 upon binding to micellar interfaces in the absence and presence of competitive inhibitors. a 1h and 15n nmr study.	an nmr study has been made of porcine pancreatic phospholipase a2 (pla) in three environments: free in solution, in a binary complex with dodecylphosphocholine micelles, and in a ternary complex with a micelle and the substrate-like inhibitor (r)-1-octyl-2-(n-dodecanoylamino)-2-deoxyglycero-3-phosph oglycol. 1h and 15n chemical shifts, amide exchange rates, and noe intensities are compared for the enzyme in different environments. from these data, structural differences are found for the n-termi ...	1992	1390760
hiv antibodies in babies.		1992	1392904
post-antibiotic effect of the new streptogramin rp 59500.	the post-antibiotic effect (pae) of rp 59500, a new streptogramin antibiotic, was determined for staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, streptococcus pneumoniae, and streptococcus pyogenes. a 30 min exposure of staphylococcus aureus to 5 micrograms/ml of rp 59500 produced a pae of 1.9-6.9 h, and a 60 min exposure of 2.5 micrograms/ml produced a pae of 3.2-8 h. a 30 min exposure of 5 micrograms/ml of rp 59500 of coagulase-negative staphylococci produced a ...	1992	1396777
in-vitro activity of streptogramin rp 59500 against staphylococci, including bactericidal kinetic studies.	the in-vitro activity of rp 59500 and comparative agents was determined against 270 clinical isolates of the genus staphylococcus. mics were performed by micro-dilution dilution. mic90 and mbc90 (mg/l) of rp 59500 were as follows: oxacillin-sensitive staphylococcus aureus (0.5/0.5), oxacillin-resistant s. aureus (0.5/0.5), oxacillin-sensitive staphylococcus epidermidis (0.5/0.5), oxacillin-resistant s. epidermidis (0.25/0.25), oxacillin-resistant staphylococcus hominis (1.0/1.0), oxacillin-resis ...	1992	1399952
in vitro activity of mersacidin (m87-1551), an investigational peptide antibiotic tested against gram-positive bloodstream isolates.	we measured the in vitro activity of mersacidin (formerly m87-1551) against 183 clinical isolates (vancomycin susceptible) and 12 additional vancomycin-resistant strains of gram-positive bacteria. the activity for mersacidin increased an average twofold (range, 1.7- to 7.6-fold) in a calcium-enriched medium. the minimum inhibitory concentration (mic)90 for mersacidin was 8-32 times higher than vancomycin for staphylococci, 4-64 times higher for enterococci, and up to 32 times higher for other or ...	1992	1424522
identification of calcium-dependent phospholipid-binding proteins (annexins) from guinea pig alveolar type ii cells.	a new group of calcium-regulating proteins, called annexins or ca(++)-dependent phospholipid-binding proteins (plbp), have been detected in different species, organs and cell types. in the present study, we have identified and quantitated plbp from guinea pig lung, lavage fluid and alveolar type ii cells to elucidate the possible role of plbp in lung surfactant biogenesis and secretion. lungs were lavaged and type ii cells from lavaged lung were isolated by elastase digestion and purified by cen ...	1992	1435768
phage pattern and antibiotic resistance pattern of coagulase-negative staphylococci obtained from immunocompromised patients.	a total of 152 coagulase-negative staphylococcal strains were isolated from clinical samples of 14 patients hospitalized after bone-marrow transplantation in a specialized hospital ward in hungary, during an 18-month period between 1987 and 1989. two species, staphylococcus epidermidis and staphylococcus haemolyticus, predominated (each, 45%). using pulverer and co-workers' phage set for typing, 68% of the isolates were typable; 16 phage patterns were observed. a characteristic long pattern with ...	1992	1461152
in vitro adherence of staphylococcus saprophyticus, staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus aureus to human ureter.	staphylococcus saprophyticus adhered to human ureteral epithelium in vitro. the levels of adherence, which were determined quantitatively with the scanning electron microscope, correlated well with bacterial hemagglutinating activities with sheep erythrocytes (r = 0.9459, p < 0.01). transmission electron microscopy revealed that the adhering bacteria and the hemagglutinating bacteria possessed similar pili-like structures on their cell surfaces. staphylococcus epidermidis, staphylococcus haemoly ...	1992	1462477
dielectric properties of human glioma and surrounding tissue.	dielectric properties of human glioma and surrounding tissue from five patients were measured. experiments were performed at frequencies of 5-500 mhz at 24 +/- 0.5 degrees c using a network analyser and a coaxial line capacitive sensor. the permittivity and conductivity of tumours were 30% higher than that of the surrounding tissues due to their higher water content. the characteristic of less differentiation in tumours clearly was reflected in the dielectric properties, namely a smaller paramet ...	1992	1479201
experimental podophyllotoxin (bajiaolian) poisoning: ii. effects on the liver, intestine, kidney, pancreas and testis.	young male rats were orally intubated with podophyllotoxin: group i, control animals, orally fed with vehicle only; group ii, fed with an initial dose of 5 mg.kg-1 b.w., followed by a daily dose of 1.67 mg.kg-1 b.w. for 7d. group iii, fed with an initial dose of 15 mg.kg-1 b.w., followed by a daily dose of 5 mg.kg-1 b.w. for 7d. all animals were sacrificed 72 h after the last dosing. histopathological examination revealed dose-related fatty change of the liver, atrophy and degenerative changes o ...	1992	1489523
[multi-resistant strains of salmonella typhi in spain].	most salmonella typhi isolated in spain are susceptible to antibiotics commonly used in its treatment as chloramphenicol, ampicillin and cotrimoxazole. three multiresistant strains have been isolated from different patients the last two years. two phage type m1, biotype xylose tetrationate + strains were isolated from blood of two patients in bembibre (león). one phage type e1a biotype xylose + tetrationate reductase + strain was isolated from blood and faeces of one patient in barcelona. all st ...	1992	1489795
in vitro activity of ro 23-9424, a dual-action antibacterial agent, against bacterial isolates from cancer patients compared with those of other agents.	the in vitro activity of ro 23-9424 against bacterial isolates from patients with cancer was compared with those of fleroxacin, ciprofloxacin, cefoperazone, and ceftazidime. ro 23-9424 inhibited the majority of the members of the family enterobacteriaceae and all aeromonas isolates at a concentration of less than or equal to 1.0 micrograms/ml. it was also active against acinetobacter spp. and haemophilus influenzae, including beta-lactamase-producing strains. the mic for 90% of isolates (mic90) ...	1992	1503453
in-vitro activities of 14-, 15- and 16-membered macrolides against gram-positive cocci.	the in-vitro activities of the 14-membered macrolides erythromycin, dirithromycin, roxithromycin, clarithromycin, the 15-membered compound azithromycin and the 16-membered macrolides (16 mm) josamycin, spiramycin and midecamycin acetate (mom) have been compared against staphylococci, enterococci and streptococci. results have been analysed separately according to the sensitivity status of the tested strains to erythromycin, namely sensitive (s), inducibly resistant (ir) or constitutively resista ...	1992	1506329
in vitro activities of three semisynthetic amide derivatives of teicoplanin, mdl 62208, mdl 62211, and mdl 62873.	mdl 62208, mdl 62211, and mdl 62873 are three semisynthetic amide derivatives of teicoplanin (mdl 62208 is an amide of teicoplanin aglycone, mdl 62211 is an amide of the teicoplanin a2 complex, and mdl 62873 is the corresponding derivative of peak a2-2 of the complex). the three semisynthetic glycopeptides were evaluated for in vitro antibacterial activity in comparison with the parent drug (teicoplanin) and vancomycin. a variety of gram-positive bacteria of clinical origin, whose species were c ...	1992	1534977
comparative therapeutic efficacy of teicoplanin and vancomycin in normal and in neutropenic mice infected with staphylococcus haemolyticus.		1992	1535069
an endemic strain of staphylococcus haemolyticus colonizing and causing bacteremia in neonatal intensive care unit patients.	coagulase-negative staphylococci are now the major cause of bacteremia in neonatal intensive care units. to date, coagulase-negative staphylococci causing neonatal infections have been found to be distinct when typed by standard techniques. to determine whether or not an endemic strain could be identified using more discriminatory techniques, we characterized coagulase-negative staphylococci isolates obtained from a prospective study of coagulase-negative staphylococci bacteremia in a neonatal i ...	1992	1557264
rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures.	urate oxidase (ec 1.7.3.3), which catalyzes the oxidation of uric acid to allantoin, is present in most mammals but absent in humans and hominoid primates. in rats and most other mammals that catabolize uric acid to allantoin, this enzyme is localized within the crystalloid cores of peroxisomes present in liver parenchymal cells. to determine whether urate oxidase forms these crystalloid cores or whether core-forming protein(s) exist in association with urate oxidase, a baculovirus expression ve ...	1992	1594592
a dna-probe for the detection of the species staphylococcus haemolyticus.	a 1.3-kb dna fragment isolated from staphylococcus haemolyticus strain dsm 20264 can be used as a specific probe for this species. the probe hybridized with 39 clinical isolates of s. haemolyticus but not with any of the 121 isolates representative of the other 25 species of staphylococci described to date.	1992	1612422
[study of the virulence of coagulase-negative staphylococci in experimental infections].	the pathogenicity and virulence of 3 strains of staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus saprophyticus, respectively were studied in balb/c mice by intraperitoneal (ip) challenge using 4 bacterial suspensions of different colony forming units (cfu) of each strain. strains were isolated from wound, blood, and urine of inpatients. on the base of the lethality rates, s. saprophyticus proved to be the most virulent (ld50 = 2.7-2.9 x 10(7) cfu/g body wt), while the s ...	1992	1625850
[a staphylococcus haemolyticus study in urinary infections. an analysis of 8 cases].	urinary infections caused by staphylococcus are attributed usually to staphylococcus epidermidis or staphylococcus saprophyticus. 8 cases of urinary infection due to s. haemolyticus are discussed: 5 adults all of them over 66 years, diagnosed respectively of diabetes, cancer and stroke. three children diagnosed of febrile syndrome, encephalopathy and enterocolitis associated with urinary infection. s. haemolyticus was identified through the determination of 19 biochemical parameters. the antibio ...	1992	1626088
in vitro antimicrobial activity of cp-99,219, a novel azabicyclo-naphthyridone.	cp-99,219 is a trifluoronaphthyridone with significant antibacterial activity that includes the family enterobacteriaceae (mics for 90% of the strains tested [mic90s], < or = 0.015 to 0.5 micrograms/ml), moraxella catarrhalis, haemophilus influenzae, and gonococci (mics, < or = 0.015 micrograms/ml). legionella spp. were also cp-99,219 susceptible, with mics of 0.008 to 0.12 micrograms/ml. cp-99,219 demonstrated activity greater than that of ciprofloxacin, ofloxacin, or enoxacin against pseudomon ...	1993	8043036
[a molecular biology approach to the species identification of coagulase-negative staphylococci].	a 1.3 dna fragment isolated from staphylococcus haemolyticus strain dsm 20264 can be used as a specific probe for this species. 28 collection strains including type strains of 26 staphylococcus species, as well as 135 clinical isolates representing 8 species, have been studied with this probe by the dot hybridization method. the probe has been found to hybridize with all 42 s. haemolyticus strains, but with none of 121 strains belonging to other staphylococcus species.	1993	8067087