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spatial and temporal regulations in helicoidal extracellular matrices: comparison between plant and animal systems.this paper proposes an overview of the last few years' investigations regarding the helicoid formation in extracellular matrices (ecms). despite the architectural polymorphism displayed among the layered ecm throughout the living kingdom, helicoidal structures are often described in ecms and appear as an optimal mechanical device. helicoids correspond to complex two-phases composites, formation and regulation of which are still a source of debate. taking the time-event into consideration, it is ...20061636171
inhibition of trypsin by t-butyloxycarbonyl-alpha-aza-(4-aminophenyl)alanine phenyl ester.t-butyloxycarbonyl-alpha-aza-(4-aminophenyl)alanine phenyl ester (iii: r = nh2) has been synthesized. the rate of inhibition of trypsin (ec 3.4.21.4) by this compound (due to acylation followed by slower deacylation) shows a marked ph maximum at approximately 6. the shape of the ph-rate curve is discussed in terms of (i) the normal ph-activity curve of trypsin reacting with a charged substrate, i.e. the protonated form of the amino compound, (ii) the deprotonation of the 4-amino group with pka 4 ...20061367228
unilateral headache with features of hemicrania continua and cervicogenic headache--a case report.a case is presented which has features of cervicogenic headache and of hemicrania continua. a sudden maneuver of the neck and later a greater occipital nerve block, both resulted in relief of the pain. a cervical cause is suggested.20061446991
cost, controversy, crisis: low back pain and the health of the public. 20061828670
thoracic actinomycosis--review of 9 cases.nine cases of thoracic actinomycosis treated in the period from 1976 to 1990 were reviewed. among them, 4 cases were children. the clinical symptoms and laboratory examinations were non-specific. chest roentgenographic manifestations including nodular (3 cases), infiltrations (4 cases), and infiltrations with pleural changes (2 cases) were also non-specific. chest wall masses were noted in four patients. there were no cases correctly diagnosed on admission. the diagnosis of thoracic actinomycosi ...20061797368
thoracic actinomycosis--review of 9 cases.nine cases of thoracic actinomycosis treated in the period from 1976 to 1990 were reviewed. among them, 4 cases were children. the clinical symptoms and laboratory examinations were non-specific. chest roentgenographic manifestations including nodular (3 cases), infiltrations (4 cases), and infiltrations with pleural changes (2 cases) were also non-specific. chest wall masses were noted in four patients. there were no cases correctly diagnosed on admission. the diagnosis of thoracic actinomycosi ...20061797368
successful introduction of an intravenous line insertion team at a municipal hospital.an intravenous line insertion and blood drawing team was introduced as a pilot project in a large municipal hospital in the new york city. the program successfully accomplished the goals of reducing house staff workload, improving house staff morale and patient care, and increasing available time for house staff education.20061800767
successful introduction of an intravenous line insertion team at a municipal hospital.an intravenous line insertion and blood drawing team was introduced as a pilot project in a large municipal hospital in the new york city. the program successfully accomplished the goals of reducing house staff workload, improving house staff morale and patient care, and increasing available time for house staff education.20061800767
treatment of multiple intracranial aneurysms in the anterior circulation--case report.a 69-year-old female underwent surgery for subarachnoid hemorrhage due to a ruptured intracranial aneurysm. nine additional aneurysms were found. four of the latter nine aneurysms were clipped in a second operation, and two were wrapped.20061694273
external cephalic version and daily post-versional maternal self-assessment of fetal presentation. a prospective study.external cephalic version (ecv) using tocolysis is a widely recognized low-risk intervention in cases of breech presentation, particularly in areas where resources are scarce and the perinatal mortality is high in vaginal breech deliveries. in such a setting, pregnant women were trained to make regular self-assessments of the presenting fetal part after ecv in order to detect spontaneous reversions to breech presentation. it was found that such maternal involvement could significantly improve th ...20061563651
medical waste: science vs politics. 20061539964
the scope of professional practice. 20061483017
capture, mutate, quantitate: variations of a central pcr scheme. 20061472383
[the comprehensive diagnosis and treatment of lymphosarcoma of the pharynx].modern methods (intrascopic and other instrumental) were used to increase the diagnostic efficiency and extension of the tumour process in 22 patients with lymphosarcoma of the pharynx. optimal treatment efficacy is achieved in lymphosarcoma of the pharynx by combined use of surgical, radiation (non-traditional course) treatment as well as chemotherapy which is used at early stages as an adjunctive method.20061448998
promoting sexual health. 20061392896
gluten breakdown by lactobacilli and pediococci strains isolated from sourdough.to evaluate the growth and metabolic activity of lactobacilli and pediococci strains in a gluten base medium (gbm), formulated for a proper selection of proteolytic strains to be used in sourdough fermentation.200616620203
fermentation of wheat: effects of backslopping different proportions of pre-fermented wheat on the microbial and chemical composition.the objective of the study was to examine effect of backslop on the chemical and microbiological characteristics of fermented wheat (fw). coarsely ground wheat was mixed with water (1:3 wt/wt) and inoculated with 6 log cfu ml(-1) each of an overnight culture of lactobacillus plantarum and pediococcus pentosaceus. four fermentation treatments were conducted in 45 1, closed, pvc containers over 48 hours. three treatments investigated the benefits of the addition of previously fermented wheat (back ...200616649578
elimination of escherichia coli o157:h7 from fermented dry sausages at an organoleptically acceptable level of microencapsulated allyl isothiocyanate.four sausage batters (17.59% beef, 60.67% pork, and 17.59% pork fat) were inoculated with two commercial starter culture organisms (>7 log(10) cfu/g pediococcus pentosaceus and 6 log(10) cfu/g staphylococcus carnosus) and a five-strain cocktail of nonpathogenic variants of escherichia coli o157:h7 to yield 6 to 7 log(10) cfu/g. microencapsulated allyl isothiocyanate (ait) was added to three batters at 500, 750, or 1,000 ppm to determine its antimicrobial effects. for sensory analysis, separate b ...200616672446
plasmid profile patterns and properties of pediococci isolated from caper fermentations.a collection comprising 14 isolates of pediococcus pentosaceus and one pediococcus acidilactici from the fermentation of caper fruits was studied. all isolates showed very similar fermentation profiles and produced a limited number of exoenzymes. all isolates carried large plasmids of diverse sizes between 20 and 55 kb, while some also contained smaller plasmids between 10 and 16 kb. cluster analysis of plasmid profiles revealed four main groups with various degrees of similarities. all amino ac ...200616715824
lactobacillus plantarum and pediococcus pentosaceus starter cultures as a tool for microflora management in malting and for enhancement of malt processability.lactobacillus plantarum vtt e-78076 (e76) and pediococcus pentosaceus vtt e-90390 (e390) starter cultures were added to the steeping water of normal malting barley in order to balance the microbial community and to enhance malt processability. in this study, we also investigated the effects of lactic acid-acidified mrs-spent medium (mrs-la) on malting performance. malting trials with five different two-row barley varieties were carried out in 25 kg pilot scale. the starter cultures promoted yeas ...200616719505
lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria.genomes of gram-positive bacteria encode many putative cell-surface proteins, of which the majority has no known function. from the rapidly increasing number of available genome sequences it has become apparent that many cell-surface proteins are conserved, and frequently encoded in gene clusters or operons, suggesting common functions, and interactions of multiple components.200616723015
data mining and characterization of a novel pediocin-like bacteriocin system from the genome of pediococcus pentosaceus atcc 25745.the genome of pediococcus pentosaceus atcc 25745 contains a gene cluster that resembles a regulated bacteriocin system. the gene cluster has an operon-like structure consisting of a putative pediocin-like bacteriocin gene (termed pena) and a potential immunity gene (termed peia). genetic determinants involved in bacteriocin transport and regulation are also found in proximity to pena and peia but the so-called accessory gene involved in transport and the inducer gene involved in regulation are m ...200616735728
isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in taiwan.to isolate, characterize, and identify lactic acid bacteria (lab) in suan-tsai (fermented mustard), a traditional fermented food in taiwan.200616834599
the effect of prebiotics on production of antimicrobial compounds, resistance to growth at low ph and in the presence of bile, and adhesion of probiotic cells to intestinal mucus.screening of five bile salt-resistant and low ph-tolerant lactic acid bacteria for inhibitory activity against lactic acid bacteria and bacterial strains isolated from the faeces of children with hiv/aids. determining the effect of prebiotics and soy milk-base on cell viability and adhesion of cells to intestinal mucus.200616553737
assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine.susceptibility to 12 antibiotics was tested in 75 unrelated lactic acid bacteria strains of wine origin of the following species: 38 lactobacillus plantarum, 3 lactobacillus hilgardii, 2 lactobacillus paracasei, 1 lactobacillus sp, 21 oenococcus oeni, 4 pediococcus pentosaceus, 2 pediococcus parvulus, 1 pediococcus acidilactici, and 3 leuconostoc mesenteroides. the minimal inhibitory concentrations of the different antibiotics that inhibited 50% of the strains of the lactobacillus, leuconostoc a ...200616876896
microbiological and sensory quality of dry fermented sausages containing alginate-microencapsulated lactobacillus reuteri.the probiotic bacterium lactobacillus reuteri was added to dry sausage batter, without or after being microencapsulated in alginate using either extrusion or emulsion technology. pediococcus pentosaceus and staphylococcus carnosus were added at 7 log cfu/g as starter cultures for fermentation. the sausage batter was stuffed in 55 mm fibrous casings and fermented, with smoking, at <or=26 degrees c and 88% relative humidity (rh) for 72 h. during fermentation as well as drying at 75% rh and 13 degr ...200616884803
pediococcus stilesii sp. nov., isolated from maize grains.a gram-positive, coccus-shaped, lactic acid bacterium, strain lmg 23082t, was isolated from steeped maize grains. the organism is homofermentative and produces d- and l-lactic acid from glucose. 16s rrna gene sequence analysis revealed that the organism belongs to the genus pediococcus, with pediococcus pentosaceus and pediococcus acidilactici as nearest neighbours. genotypic fingerprinting, whole-cell protein electrophoresis, dna-dna hybridizations and physiological and biochemical tests allowe ...200616449434
growth response and modifications of organic nitrogen compounds in pure and mixed cultures of lactic acid bacteria from wine.the interactions between the proteolytic x2l strain of oenococcus oeni and the non-proteolytic 12p strain of pediococcus pentosaceus were assayed. the characteristics of cell growth, protein degradation, and amino acid production of both strains were determined in pure and mixed cultures. o. oeni showed poor cell growth and greater ability in the release of amino acids to the extracellular medium, whereas p. pentosaceus showed a higher yield in cell production with a decrease in the amino acid c ...200616467990
glucan and fructan production by sourdough weissella cibaria and lactobacillus plantarum.after a large screening on sourdough lactic acid bacteria, exopolysaccharide (eps)-forming strains of weissella cibaria, lactobacillus plantarum, and pediococcus pentosaceus were selected. after 6 days of incubation at 30 degrees c, the synthesis of eps in mrs-based broth ranged from 5.54 to 7.88 mg ml-1. eps had an apparent molecular mass of ca. 104 da. as shown by carbohydrate consumption, the synthesis of eps was found from sucrose only. two types of homopolysaccharides were synthesized: gluc ...200617177514
the effects of lactobacillus buchneri 40788 and pediococcus pentosaceus r1094 on the fermentation of corn silage.the effect of inoculating whole-plant corn at the time of harvest with lactobacillus buchneri 40788 (4 x 10(5) cfu/g of fresh forage) combined with pediococcus pentosaceus r1094 (1 x 10(5) cfu/g) on the fermentation and aerobic stability of corn silage (37% dry matter) through 361 d of ensiling was investigated. dry matter recovery was similar between treatments throughout the study except at one early time point (14 d), when treated silage had a lower recovery than untreated silage. the concent ...200616960076
benefits of a synbiotic formula (synbiotic 2000forte) in critically ill trauma patients: early results of a randomized controlled trial.since probiotics are considered to exert beneficial health effects by enhancing the host's immune response, we investigated the benefits of a synbiotics treatment on the rate of infections, systemic inflammatory response syndrome (sirs), severe sepsis, and mortality in critically ill, mechanically ventilated, multiple trauma patients. length of stay in the intensive care unit (icu) and number of days under mechanical ventilation were also evaluated.200616983476
use of real time pcr to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover.real-time polymerase chain reaction (rt-pcr) was used to quantify seven species of lactic acid bacteria (lab) in alfalfa silage prepared in the presence or absence of four commercial inoculants and in uninoculated corn stover harvested and stored under a variety of field conditions. species-specific pcr primers were designed based on reca gene sequences. commercial inoculants improved the quality of alfalfa silage, but species corresponding to those in the inoculants displayed variations in pers ...200616205920
phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the eastern himalayas.sukako maacha, gnuchi, sidra and sukuti are traditional smoked and sun-dried fish products of the eastern himalayan regions of nepal and india. a total of 40 samples of sukako maacha (14), gnuchi (6), sidra (10) and sukuti (10) were collected and were analysed for microbial load. population of lactic acid bacteria (lab) as well as aerobic mesophilic counts ranged from 4.7-8.3 to 5.1-8.5 log cfu g(-1), respectively. a total of 189 strains of lab were isolated from sukako maacha, gnuchi, sidra and ...200616278028
enumeration and identification of pediococci in powder-based products using selective media and rapid pfge.in this study, pediococci selective medium (psm) was evaluated for the enumeration of pediococcus acidilactici and pediococcus pentosaceus from probiotic animal feed and silage inoculants. psm is based on the complex basal medium mrs supplemented with cysteine hydrochloride, novobiocin, vancomycin, and nystatin. no significant change in electivity was observed when pediococci where recovered from culture or powder-based products following incubation at 37 degrees c under anaerobic conditions for ...200615925418
topical puva therapy for vitiligo.vitiligo, a pigmentary disorder of the skin, causes the appearance of white patches which are profoundly devastating to the person afflicted with this disease. this article outlines the safe use of topical puva therapy in the office for treating those patients with less than 20% skin surface involvement. this treatment has a response rate of approximately 50%.20061828681
topical puva therapy for vitiligo.vitiligo, a pigmentary disorder of the skin, causes the appearance of white patches which are profoundly devastating to the person afflicted with this disease. this article outlines the safe use of topical puva therapy in the office for treating those patients with less than 20% skin surface involvement. this treatment has a response rate of approximately 50%.20061828681
moderate and mild mental retardation in the martin-bell syndrome.a survey of children attending schools for the moderately or the mildly mentally handicapped has shown that two out of 25 boys and two out of 22 girls with idiopathic moderate mental retardation had the martin-bell syndrome, while none of 75 boys and one out of 51 girls with mild mental retardation were fraxa positive. consideration of these figures along with published studies suggests that 7% of moderate and 3.8% of mild idiopathic mental retardation in boys, and 2.5% of moderate and 3.3% of m ...20061800751
moderate and mild mental retardation in the martin-bell syndrome.a survey of children attending schools for the moderately or the mildly mentally handicapped has shown that two out of 25 boys and two out of 22 girls with idiopathic moderate mental retardation had the martin-bell syndrome, while none of 75 boys and one out of 51 girls with mild mental retardation were fraxa positive. consideration of these figures along with published studies suggests that 7% of moderate and 3.8% of mild idiopathic mental retardation in boys, and 2.5% of moderate and 3.3% of m ...20061800751
soft tissue sarcomas: experience with intraoperative brachytherapy in the conservative management.forty-eight patients with soft tissue sarcomas were treated with iridium implant after wide local excision between 1980 and 1990 at our institution. external irradiation was also given postoperatively in 33 patients with initially resectable tumours (treatment a) and preoperatively in 3 with initially unresectable tumours (treatment c); brachytherapy was the only treatment in 12 patients presenting small superficial tumours or recurring after previous irradiation (treatment b). median follow up ...20061622870
occurrence of antinuclear antibodies in an unselected pregnancy population.the occurrence of antinuclear antibodies (ana) in an unselected population of pregnant women was studied by an indirect immunofluorescence method. six women out of 116 were ana-positive (5.2%), 2 of them having a moderately high titre (1:160 and 1:320) and 1 complement-fixing ana. the antigenic specificity of ana was anti-single-stranded dna in 2 cases and rnaase-sensitive anti-extractable nuclear antigen in 1 case. two of the ana-positive women had pre-eclampsia laevis and 1 had a premature del ...20061563653
[a case illustrating diagnostic difficulties in bronchiolo- alveolar cancer]. 20072168044
[familial juvenile nephronophthisis]. 20072075033
[case report of successful surgical treatment of a duplicate aortic arch in an elderly woman]. 20073450109
lactic acid bacteria in hamei and marcha of north east india.hamei and marcha are mixed dough inocula used as starters for preparation of various indigenous alcoholic beverages in manipur and sikkim in india, respectively. these starters are traditionally prepared from rice with wild herbs and spices. samples of hamei and marcha, collected from manipur and sikkim, respectively, were analysed for lactic acid bacterial composition. the population of lactic acid bacteria (lab) was 6.9 and 7.1 log cfu/g in hamei and marcha, respectively. on the basis of pheno ...200723100653
antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms.non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ...200717049799
a survey of the microbial and chemical composition of seven semi-ripened provola dei nebrodi sicilian cheeses.the microbial and chemical composition of seven different semi-ripened (45 days) provola dei nebrodi sicilian cheese samples were assessed in order to investigate the diversity of the microbial population in cheese made from different geographical areas throughout sicily.200717897218
dna fingerprinting of lactic acid bacteria in sauerkraut fermentations.previous studies using traditional biochemical identification methods to study the ecology of commercial sauerkraut fermentations revealed that four species of lactic acid bacteria, leuconostoc mesenteroides, lactobacillus plantarum, pediococcus pentosaceus, and lactobacillus brevis, were the primary microorganisms in these fermentations. in this study, 686 isolates were collected from four commercial fermentations and analyzed by dna fingerprinting. the results indicate that the species of lact ...200717921264
susceptibility of pediococcus spp. to antimicrobial agents.to investigate the susceptibility of pediococcus species to antimicrobial agents.200717241343
identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations.presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the abruzzo region (central italy) and subjected to phenotypic and genotypic characterization. a total of 21 isolates, recognized as seven strains by randomly amplified polymorphic dna (rapd)-polymerase chain reaction (pcr) typing, were identified by a polyphasic approach, consisting of 16s rrna gene sequencing, multiplex pcr assays and physiological features, as enterococcus faecium and pediococcus pentosaceus ...200717418310
pretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats.to study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats.200717426542
lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin.to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development.200717650194
survival of escherichia coli o157:h7 in dry fermented sausages containing micro-encapsulated probiotic lactic acid bacteria.escherichia coli o157:h7 is capable of surviving the rigorous processing steps during the manufacture of dry fermented sausages. the effect of adding two probiotic organisms, lactobacillus reuteri and bifidobacterium longum as co-cultures with the meat starter cultures pediococcus pentosaceus and staphylococcus carnosus on the viability of e. coli o157:h7 in dry fermented sausages was studied. a 5 strain cocktail of e. coli o157:h7 was added at 7.4 log cfu/g to the sausage batter and challenged ...200716943098
detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals.the production of antimicrobial activities as well as the presence of bacteriocin structural genes (enta, entb, entp, entq, cyll, entas-48, bac31, and entl50a/b) were studied in 140 non-selected faecal enterococcal isolates recovered from wild animals. eight different indicator strains (including listeria monocytogenes, pediococcus pentosaceus, and different enterococcal species) were used for antimicrobial activity detection. twenty-five of the 140 enterococci (18%) showed antimicrobial activit ...200716872815
[biochemistry of mitochondrial encephalomyopathy]. 20072223265
[biochemistry of mitochondrial encephalomyopathy]. 20072223265
[leukocyte and atherosclerosis]. 20071947989
isolated significant left main coronary artery stenosis: angiographic, hemodynamic, and clinical findings in 16 patients.among 21,545 adult patients who underwent consecutive coronary angiography, 16 (0.07%) were found during their coronary arteriography to have a significant isolated stenotic lesion (luminal diameter narrowing of 50% or more) located at the left main coronary artery. the remaining major epicardial coronary arteries and their branches were free of disease. a strong predilection for the isolated lesion to occur at the ostium of the left main artery was found (12 patients). the most common presentin ...20071950993
violent trends. 20072034554
[fractures-dislocations of the shoulder in middle-aged and elderly patients].114 patients with fracture dislocations of the proximal end of humerus have been treated and observed. 83 of them were subjected to conservative and 31--to surgical treatment. clinical, roentgenologic and radiologic evaluation of the immediate and long-term results allowed to formulate recommendations as to the selection of the treatment method of humerus fracture dislocations depending upon their type and general state of the patients.20072074957
acid and the pain of non-ulcer dyspepsia. 20071932675
no evidence for the enhanced production of insulin autoantibodies after confrontation with common viral antigens in insulin dependent diabetes mellitus.the production of insulin autoantibodies (iaa) was studied after common viral infections in 12 children with type 1 diabetes mellitus and in their 18 healthy siblings. in addition, the production of iaa was measured after influenza vaccination with booster in 39 patients with type 1 diabetes mellitus and in 39 healthy controls. in 7 of the 12 diabetic children 13 viral infections were serologically confirmed. among the siblings 14 periods of infection were noted in 9 individuals. a significant r ...20072074915
[pregnancy and cystic fibrosis. a case history].the authors report a case of pregnancy in a patient suffering from moderately severe cystic fibrosis (the syndrome consisted of diffused obstruction in respiratory function with a vital capacity of 79% and the lowered blood oxygen level with arterial blood gas estimations; oxygen saturation was 94%). the condition was marked by temporary worsening during the pregnancy in which infective bronchitis supervened). she also threatened to go into early labour and did in fact deliver at 36 1/2 weeks of ...20072258595
antilisterial activity of lactic acid bacteria isolated from "alheiras" (traditional portuguese fermented sausages): in situ assays.a total of 226 lactic acid bacteria (lab) isolated from "alheira", a traditional portuguese fermented sausage, were screened for antagonistic activity against some pathogenic microorganisms, including listeria monocytogenes. the objective was to isolate lab with antibacterial activity from "alheiras" and to select strains that could be used in "alheira" production. isolates displaying antibacterial activity against listeria innocua and l. monocytogenes were investigated for the nature of the ant ...200722061260
hospital outbreak of scabies stemming from two aids patients with norwegian scabies. 20071971073
mucinous ascites associated with rupture of benign ovarian teratoma. case report. 20072223689
mucinous ascites associated with rupture of benign ovarian teratoma. case report. 20072223689
post-concussional symptoms following minor head injury.the effect of admission to hospital after a minor head injury on the incidence and duration of post-concussional symptoms was investigated. a total of 114 adults with a minor head injury were randomly allocated to admission for 24 h or to discharge from the accident department. follow-up was by postal questionnaire. this revealed that 90 per cent of patients suffered symptoms lasting an average of 2 weeks. the patients who were discharged had symptoms that lasted a shorter time. admission to hos ...20072592090
new prosthetic venous collar anastomotic technique: combining the best of other procedures. 20071913097
redox potential to discriminate among species of lactic acid bacteria.to verify to what degree reducing capacity is a characterizing parameter of a species, and of the strains themselves within a given species, of lactic acid bacteria.200717953562
characterization and selection of autochthonous lactic acid bacteria isolated from traditional iberian dry-fermented salchichón and chorizo sausages.a total of 192 lactic acid bacteria were isolated from 2 types of naturally fermented dry sausages at 3 different stages of the ripening process in order to select the most suitable strains as starter cultures in dry-cured sausage manufacture according to their technological characteristics such as glucose fermentation, lactic and acetic acid production, and proteolytic, lipolytic, and antimicrobial activities. identification of the isolates revealed that 31.2% were pediococcus pentosaceus, 26.9 ...200717995686
microbial development in distillers wet grains produced during fuel ethanol production from corn (zea mays).distillers grains are coproduced with ethanol and carbon dioxide during the production of fuel ethanol from the dry milling and fermentation of corn grain, yet there is little basic microbiological information on these materials. we undertook a replicated field study of the microbiology of distillers wet grains (dwg) over a 9 day period following their production at an industrial fuel ethanol plant. freshly produced dwg had a ph of about 4.4, a moisture content of about 53.5% (wet mass basis), a ...200718026225
phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of north east india.mesu, soidon, soibum and soijim are ethnic fermented bamboo tender shoot products prepared by the people in north east india. microbiological analysis of mesu, soidon, soibum and soijim showed the population dominated by lactic acid bacteria (lab) ranging up to 10(8) cfu g(-1). the phenotypic characterisation of predominant lab isolated from the fermented bamboo shoot products was based on general morphology, physiological tests, api and biolog systems. the genotypic characterisation of lab was ...200818036695
method for reliable isolation of lactobacillus sakei strains originating from tunisian seafood and meat products.in tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. we isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of tunisia, and selectively searched for lactobacillus sakei, a lactic ...200818155310
population diversity of yeasts and lactic acid bacteria in pig feed fermented with whey, wet wheat distillers' grains, or water at different temperatures.the diversity of populations of yeast and lactic acid bacteria (lab) in pig feeds fermented at 10, 15, or 20 degrees c was characterized by rrna gene sequencing of isolates. the feeds consisted of a cereal grain mix blended with wet wheat distillers' grains (wwdg feed), whey (w feed), or tap water (wat feed). fermentation proceeded for 5 days without disturbance, followed by 14 days of daily simulated feed outtakes, in which 80% of the contents were replaced with fresh feed mixtures. in wwdg fee ...200818223110
nisin antimicrobial activity and structural characteristics at hydrophobic surfaces coated with the peo-ppo-peo triblock surfactant pluronic f108.the antimicrobial peptide nisin has been observed to preferentially locate at surfaces coated with the poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (peo-ppo-peo) surfactant pluronic f108, to an extent similar to its adsorption at uncoated, hydrophobic surfaces. in order to evaluate nisin function following its adsorption to surfaces presenting pendant peo chains, the antimicrobial activity of nisin-loaded, f108-coated polystyrene microspheres and f108-coated polyurethane cathe ...200818377919
inhibition of escherichia coli o157:h7 in ripening dry fermented sausage by ground yellow mustard.compounds generated by the enzymatic hydrolysis of glucosinolates naturally present in mustard powder are potently bactericidal against escherichia coli o157:h7. because e. coli o157:h7 can survive the dry fermented sausage manufacturing process, 2, 4, and 6% (wt/wt) nondeheated (hot) mustard powder or 6% (wt/wt) deheated (cold) mustard powder were added to dry sausage batter inoculated with e. coli o157:h7 at about 7 log cfu/g to evaluate the antimicrobial effectiveness of the powders. reductio ...200818389690
transport of glycerol by pediococcus pentosaceus isolated from wine.pediococcus pentosaceus n(5)p is a strain isolated from wine that uses glycerol as its sole carbon source, mainly via the glycerol kinase pathway. the transport of glycerol was investigated in resting cells of this strain. glycerol uptake followed a michaelis-menten relationship with an observed apparent k(m) of 33 microm and a v(max) of 2.5 nmol/min/mg of cell protein. the transport system was specific for glycerol, which was present in the cells grown either on glycerol or glucose suggesting i ...200818456108
isolation and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi.screening and partial characterization of a bacteriocin produced by pediococcus pentosaceus k23-2 isolated from kimchi, a traditional korean fermented vegetable.200818540969
a factory-scale application of secondary adjunct cultures selected from lactic acid bacteria during puzzone di moena cheese ripening.the lactic acid populations of 2 seasonal puzzone di moena cheeses made from winter and summer raw cow's milk were characterized at different ripening times. lactic acid bacteria (lab) were isolated on selective media and subjected to genetic typing and identification. the species most frequently found during ripening were lactobacillus paracasei ssp. paracasei, lactobacillus plantarum, and pediococcus pentosaceus. the different strains recognized by random amplification of polymorphic dna-pcr w ...200818650274
pediococcus pentosaceus nb-17 for probiotic use.the plant-derived pediococcus pentosaceus nb-17 was isolated from japanese traditional vegetable pickles. the production of cytokines from mouse spleen cells co-cultivated with heat-killed bacteria was investigated in vitro. the bacteria significantly induced secretion levels of interferon (ifn)-gamma and interleukin (il)-12 p70, and suppressed il-4 productions in ovalbumin (ova) sensitized mouse spleen cells. therefore, the bacteria could effectively stimulate immune activities and showed aller ...200818691534
selection and use of autochthonous mixed starter for lactic acid fermentation of carrots, french beans or marrows.strains of leuconostoc mesenteroides, lactobacillus plantarum, weissella soli/weissella koreensis, enterococcus faecalis, pediococcus pentosaceus and lactobacillus fermentum were identified from raw carrots, french beans and marrows by partial 16s rrna gene sequence. l. plantarum m1, leuc. mesenteroides c1 and p. pentosaceus f4 were selected based on the rates of growth and acidification in vegetable juice media, and used as the autochthonous mixed starter for the fermentation of carrots, french ...200818710789
diversity of lactic acid bacteria in two flemish artisan raw milk gouda-type cheeses.pcr-denaturing gradient gel electrophoresis (pcr-dgge) was used to study the diversity of lactic acid bacteria (lab) in two flemish artisan raw milk gouda-type cheeses. in parallel, conventional culturing was performed. isolates were identified using (gtg)(5)-pcr and sequence analysis of 16s rrna and phes genes. discriminant analysis revealed some differences in overall lab diversity between the two batches and between the two cheeses. within each batch, the diversity of 8- and 12-week-old chees ...200818721684
microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge.the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ...200818838186
lactic acid bacteria with potential to eliminate fungal spoilage in foods.to investigate antifungal activity produced by lactic acid bacteria (lab) isolated from malted cereals and to determine if such lab have the capacity to prevent fungal growth in a particular food model system.200817976175
role of hypermutability in the evolution of the genus oenococcus.oenococcus oeni is an alcohol-tolerant, acidophilic lactic acid bacterium primarily responsible for malolactic fermentation in wine. a recent comparative genomic analysis of o. oeni psu-1 with other sequenced lactic acid bacteria indicates that psu-1 lacks the mismatch repair (mmr) genes muts and mutl. consistent with the lack of mmr, mutation rates for o. oeni psu-1 and a second oenococcal species, o. kitaharae, were higher than those observed for neighboring taxa, pediococcus pentosaceus and l ...200817993526
pediococcus argentinicus sp. nov. from argentinean fermented wheat flour and identification of pediococcus species by phes, rpoa and atpa sequence analysis.a gram-positive, small coccus-shaped lactic acid bacterium, strain lmg 23999(t), was isolated from argentinean wheat flour. 16s rrna gene sequence analysis revealed that the phylogenetic position of the novel strain was within the genus pediococcus, with pediococcus stilesii, pediococcus pentosaceus and pediococcus acidilactici as its closest relatives (97.7, 97.3 and 96.9 % gene sequence similarity, respectively). fluorescent amplified fragment length polymorphism fingerprinting of whole genome ...200819060081
isolation and characterization of bacteriocin-producing bacteria from the gastrointestinal tract of broiler chickens for probiotic use.to isolate and characterize the bacteriocin-producing bacteria (bpb) from the gastrointestinal tract of broiler chickens for probiotic use.200819120666
functionality of lactic acid bacteria peptidase activities in the hydrolysis of gliadin-like fragments.to evaluate the role of the peptidase activities from sourdough lactic acid bacteria (lab) in the degradation of alpha-gliadin fragments.200819146533
outpatient geriatric evaluation.this article provides a comprehensive description of an established geriatric ambulatory consultation service at monroe community hospital in rochester, new york. the history of the service is presented and clinic staffing and operations are reviewed. descriptive information for patients evaluated during a 1-year period and the impact of this team-oriented assessment compared to assessment by physicians are provided.20083815242
prolongation of anesthesia with xylazine, ketamine, and guaifenesin in horses: 64 cases (1986-1989).on 74 occasions, 54 horses and 6 foals were anesthetized with xylazine and ketamine or xylazine, guaifenesin, and ketamine, with or without butorphanol. on 64 occasions, anesthesia was prolonged for up to 70 minutes (34 +/- 15 min) by administration of 1 to 9 supplemental iv injections of xylazine and ketamine at approximately a third the initial dosage. all horses except 5 were positioned in lateral recumbency, and oxygen was insufflated. in adult horses, the time from induction of anesthesia t ...20082276967
the aps knee joint prosthesis. a review of 32 patients.first clinical and radiographic results with a new knee joint prosthesis, implanted without cement, are reported and the characteristics of the implant (dowel fixation and special instrumentation) presented. the first series of 35 joints with a follow-up period of 1-3 years shows excellent results. radiographically, primary osseous integration cannot be positively identified in all cases, but it could be proved in most instances. cement-free implantation requires good preoperative bone quality a ...20082271356
molecular and technological characterization of lactic acid bacteria from traditional fermented sausages of basilicata region (southern italy).lactic acid bacteria (lab) from traditional fermented sausages of the basilicata region were investigated by ardra-pcr and rapd-pcr for taxonomic identification at species and strain level and characterized on the basis of the growth and acidification at different temperatures, incubation times, levels of nacl and kno(2), hydrolysis of sarcoplasmatic and myofibrillar proteins and antimicrobial, peptide/amino acid release and nitrate reductase activities. lactobacillus sakei was the predominant s ...200822063864
effect of processing methods and starter culture (staphylococcus xylosus and pediococcus pentosaceus) on proteolytic changes in turkish sausages (sucuk) during ripening and storage.proteolytic changes in turkish sausages (sucuk) produced by two methods (heat processing and traditional) were determined during processing and storage for 90 days. the sausages were produced with or without starter culture in both methods. a mixture of staphylococcus xylosus and pediococcus pentosaceus was used as starter culture for their acidic and proteolytic characteristics. the major changes in proteolytic characteristics of sucuk took place during the fermentation stage, with an increase ...200822063339
pcr amplification of dna microdissected from a single polytene chromosome band: a comparison with conventional microcloning.a novel alternative to microcloning for the production of region specific chromosomal dna is described. in this method, 'microamplification', single bands are dissected from polytene chromosomes and digested with sau3a. oligonucleotide adaptors are ligated to these fragments to provide convenient priming sites for polymerase chain reaction amplification. in this way, as much as 1 microgram of dna can be amplified from a single band. probes made from pcr amplified dna from two such dissections ha ...20082587252
the next wave: the combination of clinical work with the opportunity to serve people makes dentistry an attractive career option for women. 20082605768
the polymorphic oxidation of beta-adrenoceptor antagonists. 20082566179
hemodynamic and electrophysiologic effects of amlodipine, a new long-acting calcium antagonist: preliminary observations. 20082530867
induction of transient hyperglycaemia in cancer patients.serum glucose levels up to 700 mg/dl were achieved and maintained for 3 h in patients with advanced metastatic cancer by infusion of concentrated glucose solutions. even with infusions of large amounts of glucose, the maximum sustainable level of serum glucose did not exceed 700 mg/dl. the apparent ceiling of serum glucose level appeared to be due to a large and rapid loss of glucose from the kidneys. with adequate fluid volume replacement there was no rebound hypoglycaemia and there was no sign ...20082394924
adolescent medicine. 20082329647
ecological removal of recalcitrant phenolic compounds of treated olive mill wastewater by pediococcus pentosaceus.treatment of olive mill wastewater by different biological process led to an important decrease of organic compounds but the black coloration due to the recalcitrant phenolic compounds persists. experimental design using fractional factorial plan showed that, among the seven studied factors, yeast extract, dilution and glucose exhibited a positive effect on pediococcus pentosaceus growth and treated olive mill wastewater (tomw) decolourisation. optimization of influent factors showed that 2.5 an ...200817686628
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