Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| complement component deposition in uteroplacental (spiral) arteries in normal human pregnancy. | there is conflicting evidence for the deposition of complement in spiral arteries in normal and abnormal human pregnancies. the immunogold silver staining (igss) technique was used to investigate the distribution of c1q, c3d, c4, c6 and c9 within the spiral arteries of formalin-fixed normal pregnancy hysterectomy specimens ranging in gestational age from 4 to 40 weeks. deposition of complement components studied was observed in all cases suggesting classical pathway activation. reactivity was no ... | 1987 | 3430480 |
| invasiveness, metastasis, and transplantability of mouse liver nodules. | data are presented which show that various mouse liver nodular lesions, such as hyperplastic nodules, hepatocellular adenomas, and hepatocellular carcinomas, differ not only morphologically but also biologically regarding their behavior in the primary and secondary hosts. the hyperplastic nodules grew slowly by expansion without affecting the integrity of the lobular structure. the primary hepatocellular adenomas also grew slowly by expansion but eliminated the histoid liver structure. neither l ... | 1987 | 3472500 |
| a mixed endocrine adrenal tumour causing steatorrhoea. | a 60 year old man developed steatorrhoea, weight loss, mild diabetes mellitus, labile hypertension and limb cramps. raised plasma concentrations of catecholamines, particularly noradrenaline and a computed tomography-scan showing an adrenal tumour strongly suggested a pheochromocytoma. adrenoreceptor blockade reversed the symptoms, decreased faecal fat, and increased duodenal trypsin to normal concentrations. after adrenalectomy the patient was asymptomatic and there was no steatorrhoea. the blo ... | 1987 | 2890560 |
| glycoside conjugation in microsomes from hepatic and renal carcinoma of man. | human hepatoma which had been xenografted into nude mice have been estimated for their ability to catalyze glucuronic acid and glucose conjugation of endogenous compounds and p-nitrophenol. the xenobiotic p-nitrophenol was glucuronidated with a comparable rate in microsomes from human hepatoma, human liver and host liver. with regard to glucuronic acid or glucose conjugation of the endogenous compounds of bile acids, bilirubin and steroid hormones, glucosidation of bile acids was the only conjug ... | 1987 | 2820859 |
| glycoside conjugation in microsomes from hepatic and renal carcinoma of man. | human hepatoma which had been xenografted into nude mice have been estimated for their ability to catalyze glucuronic acid and glucose conjugation of endogenous compounds and p-nitrophenol. the xenobiotic p-nitrophenol was glucuronidated with a comparable rate in microsomes from human hepatoma, human liver and host liver. with regard to glucuronic acid or glucose conjugation of the endogenous compounds of bile acids, bilirubin and steroid hormones, glucosidation of bile acids was the only conjug ... | 1987 | 2820859 |
| differential examination of bronchoalveolar lavage cells in tobacco cigarette and marijuana smokers. | cells derived from bronchoalveolar lavage (bal) were obtained from 19 healthy non-smokers (ns) and from 43 healthy smokers of tobacco and/or marijuana. thirteen subjects smoked tobacco cigarettes only (ts) (mean +/- se: 17.4 +/- 5.5 pack-years). thirty subjects smoked marijuana; of these, 14 smoked marijuana only (ms) (149.1 +/- 102.7 joint-years) and 16 smoked marijuana and tobacco (mts) (43.3 +/- 7.2 joint-years and 18.4 +/- 3.2 pack-years). cell counts were expressed as total number recovered ... | 1987 | 3592403 |
| differential examination of bronchoalveolar lavage cells in tobacco cigarette and marijuana smokers. | cells derived from bronchoalveolar lavage (bal) were obtained from 19 healthy non-smokers (ns) and from 43 healthy smokers of tobacco and/or marijuana. thirteen subjects smoked tobacco cigarettes only (ts) (mean +/- se: 17.4 +/- 5.5 pack-years). thirty subjects smoked marijuana; of these, 14 smoked marijuana only (ms) (149.1 +/- 102.7 joint-years) and 16 smoked marijuana and tobacco (mts) (43.3 +/- 7.2 joint-years and 18.4 +/- 3.2 pack-years). cell counts were expressed as total number recovered ... | 1987 | 3592403 |
| department of health and human services, public health service, national institutes of health. recombinant dna advisory committee. minutes of meeting, september 29, 1986. | 1987 | 3475740 | |
| tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells. | when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ... | 1987 | 3440028 |
| cytotoxic escherichia coli. | 1987 | 3305326 | |
| differential regulation of amyloid-beta-protein mrna expression within hippocampal neuronal subpopulations in alzheimer disease. | we have mapped the neuroanatomical distribution of amyloid-beta-protein mrna within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey (macaca fascicularis), normal aged human, and patients with alzheimer disease. amyloid-beta-protein mrna appears to be expressed in all hippocampal neurons, but at different levels of abundance. in the central nervous system of monkey and normal aged human, image analysis shows that neurons of the dentate gyrus and cornu ammonis fields ... | 1988 | 3277189 |
| characterization of the copper-thiolate cluster in yeast metallothionein and two truncated mutants. | cu-metallothionein was purified from saccharomyces cerevisiae harboring plasmids containing mutated cup1 metallothionein genes resulting in deletions at the carboxy-terminal end of the polypeptide. the truncated polypeptides are recovered as polypeptides of 35 and 48 residues in length. the cu-s cluster in the wild-type metallothionein and the two truncates were characterized. the truncated proteins, designated t35 and t48, contain 4 and 2 fewer cysteinyl residues, respectively, compared to the ... | 1988 | 3283130 |
| activation of the estrogen receptor from n-nitrosomethylurea-induced rat mammary tumors. | the activation of the estrogen receptor (er) from n-nitrosomethylurea (nmu)-induced rat mammary tumors was studied in vitro. the activation of the receptor induced by heating of the cytosol containing occupied er was measured by a 3-4-fold increase of receptor binding to nuclei in comparison with the nuclear binding of the nonactivated er. the activation of the er was further shown by alteration of the elution profile from deae-cellulose. a shift of the receptor peak from 234 mm (peak ii, nonact ... | 1988 | 3347053 |
| isolation of a cdna that encodes the peptide core of the secretory granule proteoglycan of rat basophilic leukemia-1 cells and assessment of its homology to the human analogue. | it has been previously shown that a single gene is used to encode the peptide core of the extracellular proteoglycan of rat l2 yolk sac tumor cells and the intracellular proteoglycan of rat basophilic leukemia (rbl)-1 cells. in order to determine if the predicted amino acid sequences of these proteoglycans are identical as well as to isolate a full length cdna encoding a rat secretory granule proteoglycan, a cdna library was prepared from rbl-1 cells and screened with the 165-base pair 5'----xmn ... | 1988 | 3366780 |
| [analysis of membrane heterogeneity and non-linearity of summation of postsynaptic potentials in a simulation model of the purkinje cell]. | propagation and summation of epsp was studied by means of a numerical model of rat purkinje neuron. it was shown that the difference between the membrane resistivity of the soma and dendrites can be determined experimentally by the character of epsp attenuation, while comparing intra- and extracellular recordings. it was also shown that at an increase of synaptic input the nonlinear increase of the potential is more pronounced in the soma that in the synaptic region. the role of this fact for th ... | 1988 | 3370230 |
| the effect of trypanosoma vivax and trypanosoma congolense infections on the reaction time and semen characteristics in the zebu bull. | 1988 | 3167554 | |
| alpha-lactalbumin production by rat mammary gland: prepartum emergence of resistance to glucocorticoids and progestins. | explants of mammary glands from pregnant or lactating rats were shown to differ markedly in their response to glucocorticoid and progestin in terms of alpha-lactalbumin production. tissue from day 14 pregnant rats had low initial tissue levels and basal production of alpha-lactalbumin, and showed a dose-biphasic response to glucocorticoids, with significantly higher production at 3 nm than 300 nm ru26988; the progestin org 2058 inhibited production. in explants from lactating rats (day 1 and day ... | 1988 | 3208993 |
| pregnancy rate and foetal mortality in aleutian disease virus infected mink. | 1988 | 3223478 | |
| [toxicologic properties and the nature of the combined effect of a orthomenthane hydrocarbon mixture]. | 1988 | 3234846 | |
| in situ optical spectroscopy of some systems of biological interest. | monitoring the optical absorption or emission spectrum of a condensed phase sample offers information about the supramolecular assembly, packing effects and other features characteristic of the phase that would be missed when one studies solution-state spectra. we have used the technique of photoacoustic spectroscopy to study intact biological specimens, such as algae, parasite cells and the eye lens. such a study has offered information about the status of endogenous hemin in plasmodium cells a ... | 1988 | 3242637 |
| production and purification of anthrax toxin. | 1988 | 3148094 | |
| [the thymus in exposure to pain]. | the effect of isotonic solution and microtrauma of the gastrocnemius muscle to the structure and cell composition in various zones of the thymus has been studied on mature white male rats on the first, 7th and 14th days of the experiment. at injection of the isotonic solution (sodium chloride) the part of small lymphocytes decreases and that of the large and middle ones--increases (it is mostly manifested on the 7th day of the experiment). the amount of immature plasma cells increases sharply at ... | 1988 | 3415484 |
| effect of na+/h+ exchange inhibitors on agonist-induced contraction of rat aorta. | a number of vasoconstrictor agonists activate the na+/h+ antiport system in vascular smooth muscle, leading to alkalinization of the cytosol and influx of na+. it is believed that agonist-induced na+/h+ exchange may play an important role in contraction. we have evaluated this hypothesis by determining the effect of inhibition of na+/h+ exchange on phenylephrine (pe)-induced contraction of rat aorta. preincubation of rat aorta with the na+/h+ exchange inhibitor amiloride (0.5 mm) inhibited subse ... | 1988 | 2849662 |
| initiation and characterization of primary mouse kidney epithelial cultures. | primary cultures of murine renal epithelial cells were established from a preparation of proximal tubule fragments. confluent cultures exhibited multiple dome formation, indicating the presence of tight junctions and an intact transcellular transport process. ultrastructural analysis revealed a monolayer of polarized cells, with a sparse but clearly defined microvillar surface facing the growth medium and a basolateral surface attached to the substratum. cultures grown on collagen gels did not s ... | 1988 | 2840432 |
| stimulation of growth hormone secretion by central administration of atrial natriuretic polypeptide in the rat. | intracerebroventricular (icv) injection of alpha-human atrial natriuretic polypeptide (alpha hanp) or alpha-rat anp (0.6 and 3 nmol/rat) elicited an increase in plasma gh levels both in conscious freely moving rats and in urethane-anesthetized rats when given at the trough of spontaneous gh secretion. antiserum specific for rat grf did not affect the plasma gh increase induced by icv injection of alpha hanp. intracerebroventricular injection of alpha anp (3 nmol/rat) failed to stimulate gh secre ... | 1988 | 2966063 |
| differential effects of (-)-norepinephrine and of (+/-)-isoproterenol on cardiac contraction, an uptake phenomenon. | concentration-dependent inotropic effects of (-)-norepinephrine and of (+/-)-isoproterenol were compared in isometrically contracting guinea pig papillary muscles stimulated at a frequency of 0.2 hz. (-)-norepinephrine (0.1-100 mumol/l) elicited a positive inotropic effect that was not antagonized by carbachol, failed to produce a positive inotropic staircase after resumption of stimulation and shortened relaxation time in a concentration-dependent fashion.(+/-)-isoproterenol had a dual action: ... | 1988 | 2849663 |
| structural organization of the mouse mitochondrial malate dehydrogenase gene. | structural organization of the mouse mitochondrial malate dehydrogenase (ec 1.1.1.37) gene was determined by analyzing a genomic dna fragment isolated from a cosmid library. the gene is 12,000 base-pairs long and contains nine exons interrupted by eight introns of various sizes. the 5' and 3'-flanking regions, and the exact sizes and boundaries of the exon blocks including the transcription-initiation sites were determined. in the 5'-flanking region, there is neither a tata box nor a caat box. i ... | 1988 | 3379635 |
| effect of different modes of dialysis on serum erythropoietin levels in pediatric patients. a report of the southwest pediatric nephrology study group. | the relative importance of erythropoietin (ep) and inhibitors of erythropoiesis in the development of anemia in pediatric patients with end-stage renal disease (esrd) was assessed in 82 patients: 41 treated with peritoneal dialysis (pd) and 41 with hemodialysis (hd). serum ep was determined with a sensitive radioimmunoassay. potential serum inhibition of erythroid (cfu-e) and granulocytic (cfu-gm) progenitor cell growth was assessed using human bone marrow cell cultures. the mean ep level for al ... | 1988 | 3153056 |
| isolation and characterization of chondroitin 6-sulfate proteoglycans present in the extracellular matrix of rabbit bone marrow. | the authors' previous studies showed that the glycosaminoglycans present in rabbit bone marrow were composed of chondroitin 6-sulfate (79%) and hyaluronic acid (16%). immunohistochemically the chondroitin 6-sulfate was demonstrated to be in bone marrow matrix constructing hematopoietic microenvironment. in this study the authors isolated and characterized these glycosaminoglycans in their macromolecular form (i.e., proteoglycans). bone marrow of 3-month-old rabbits was defatted with organic solv ... | 1988 | 3135865 |
| acceleration of starvation- and glycerol-induced myxospore formation by prior heat shock in myxococcus xanthus. | the effect of heat shock on myxococcus xanthus was investigated during both glycerol- and starvation-induced development. cells heat shocked at 40 degrees c for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees c. additionally, m. xanthus cells heat shocked prior to glycerol induction formed a greater total number of myxospores when sporulation was complete than did control cells maintained at 30 degrees c. however, in starvation-induce ... | 1988 | 3141380 |
| characterization of a-i-containing lipoproteins in subjects with a-i milano variant. | the a-i milano variant of apolipoprotein a-i (a-im), by virtue of its arg-173----cys substitution, is capable of forming a disulfide bond with the 77-amino-acid apolipoprotein a-ii polypeptide (a-iis) as well as with itself to produce dimers, a-im/a-iis and a-im/a-im, respectively. a-i-containing lipoproteins (lp): particles with a-ii (lp(a-i with a-11)) and particles without a-ii (lp(a-i without a-ii)) in the plasma of two nonhyperlipidemic a-im carriers were investigated to determine the effec ... | 1988 | 3129016 |
| recovery of mitomycin c-treated mouse 10t1/2 cells during confluent holding. | the relationship between cytotoxicity, sister-chromatid exchanges (sce) and the repair of dna crosslinks was studied in mouse 10t1/2 cells during confluent holding following either acute or protracted mmc treatment. no cytotoxic effects were observed with increasing doses of mmc until sce frequencies 1.8 times background levels were induced. protracted mmc treatments were less cytotoxic than acute mmc exposure at doses which yielded similar frequencies of sce. the kinetics of recovery during con ... | 1988 | 3127697 |
| arachidonic acid, prostaglandin e2 and f2 alpha inhibit leucine degradation in chick skeletal muscle. | arachidonic acid (5 microm), prostaglandin e2 (0.28 microm) and f2 alpha (14 microm) inhibited (p less than 0.01) the rates of net leucine transamination, leucine oxidative decarboxylation and total co2 production from leucine in extensor digitorum communis muscles from fed ten-day-old chicks. indomethacin (50 microm) markedly inhibited (p less than 0.01) the rate of pge2 production in the presence of 5 microm arachidonic acid and prevented the inhibition of leucine degradation by arachidonic ac ... | 1988 | 3130060 |
| ethanol and its novel metabolites inhibit insulin action on adipocytes. | the effects of ethanol, acetaldehyde, and the two novel metabolites of ethanol 2,3-butanediol and 1,2-propanediol on basal and insulin-stimulated adipocyte lipogenesis and glucose oxidation in vitro were investigated. whereas ethanol and acetaldehyde inhibited both processes at concentrations far greater than those found in alcoholic subjects, the two diols were extremely potent inhibitors of basal and insulin-stimulated adipocyte metabolism at concentrations far below those observed in alcoholi ... | 1988 | 3133260 |
| regulation of intestinal apolipoprotein b synthesis and secretion by caco-2 cells. lack of fatty acid effects and control by intracellular calcium ion. | to investigate the mechanism of control of intestinal apolipoprotein b (apob) secretion, we studied the effects of fatty acids and calcium ionophores on the human intestinal model cell line caco-2. although treatment with various fatty acids (18:1w9, 18:2w6, and 20:5w3) complexed to bovine serum albumin resulted in a dramatic redistribution of apob-100 from the low density and high density lipoproteins to the very low density lipoprotein fraction, there was no effect of any of the fatty acids on ... | 1988 | 3125180 |
| effects of the antimicrobic tiamulin on seven gram-negative bacterial fish pathogens. | in vitro and in vivo test were carried out with tiamulin and gram-negative bacterial pathogens of fish. determination of minimum inhibitory concentration for 51 strains of seven species of gram-negative bacterial pathogens showed that only strains of vibrio anguillarum were sensitive at 1.6-6.25 ppm, while the rest of test strains required 25- greater than 100 ppm. control of infection was not achieved when tiamulin was fed for 14 days at 5 or 50 mg/kg to rainbow trout (salmo gairdneri) experime ... | 1988 | 3127604 |
| effects of peptide hi on basal and stimulated insulin and glucagon secretion in the mouse. | peptide hi (phi) is a peptide with 27 amino acids that is structurally similar to vip (vasoactive intestinal peptide). since phi, like vip, has been demonstrated to occur in intrapancreatic neurons, and since vip earlier was shown to stimulate insulin and glucagon secretion in the mouse, we investigated whether also phi affects islet hormone secretion in this species. phi was thereby injected intravenously at dose levels between 0.5 and 8.0 nmol/kg. it was found that phi did not affect basal lev ... | 1988 | 3047591 |
| [alcohol and endocrinologic homeostasis]. | in addition to direct toxic effects on endocrine organs chronic alcohol intake affects regulation of endocrine systems by disturbed liver function. as a result in patients with alcohol-induced liver cirrhosis gonadal axis is characterized by low total and free testosterone, elevated estradiol. lh, fsh, and sexual hormone binding globulin and an enhanced conversion of testosterone to estradiol. prolactin also is found to be elevated. the thyrotropic axis is characterised by low t3- und t4- as wel ... | 1988 | 3062942 |
| [the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"]. | 1988 | 3067282 | |
| [the activity of the control de vectores during the 50th anniversary year of the instituto de medicina tropical "pedro kourí"]. | 1988 | 3067282 | |
| a one-step preparative method for separating ser 6-phosphorylated hmg 14 from unphosphorylated hmg 14 and in vitro phosphorylation reaction components. | while clear evidence exists for the regulation of the phosphorylation of the very basic high mobility group (hmg) and histone chromatin proteins, the physiological role of their phosphorylation remains poorly understood. elucidation of these roles has been difficult, in part, because of the inability to obtain sufficient quantities of purified phosphorylated derivatives. we have used mono s cation-exchange chromatography to prepare milligram quantities of pure ser 6-phosphorylated hmg 14 (ser 6- ... | 1988 | 2839054 |
| isolation and mapping of a polymorphic dna sequence (pmcoc12) on chromosome 14 [d14s20]. | 1988 | 2899879 | |
| cns amyloid proteins in neurodegenerative diseases. | the amyloid plaques found in neurodegenerative diseases show considerable morphologic diversity. two amyloidogenic proteins have been isolated from the brains of humans and animals with neurodegenerative diseases--beta-protein from alzheimer's disease (ad) and down's syndrome, and prion protein (prp) from scrapie and creutzfeldt-jakob disease (cjd). using monoclonal antibodies to a synthetic peptide corresponding to a portion of beta-protein and rabbit antiserum to hamster scrapie prp 27-30, we ... | 1988 | 2901696 |
| gentamicin inhibits agonist stimulation of the phosphatidylinositol cascade in primary cultures of rabbit proximal tubular cells and in rat renal cortex. | a growing body of evidence indicates that aminoglycoside antibiotics interact with phosphoinositides and this has led to the hypothesis that these drugs perturb the phosphatidylinositol (pi) cascade. to test this hypothesis we examined the effect of gentamicin on agonist stimulation of the pi cascade in primary culture of rabbit proximal tubular cells (rptc) and in rat renal cortex. parathyroid (pth) (10(-6) m) stimulated a significant increase in total inositol phosphates, inositol monophosphat ... | 1988 | 2849680 |
| effects of vitamin d3 and related compounds on angiotensin converting enzyme activity of endothelial cells and on release of plasminogen activator from them. | 1988 | 2854501 | |
| effects of vitamin d3 and related compounds on angiotensin converting enzyme activity of endothelial cells and on release of plasminogen activator from them. | 1988 | 2854501 | |
| [baked porcelain]. | 1988 | 280109 | |
| [dental caries and abrasion in a series of egyptian predynastic skulls]. | 1988 | 279475 | |
| staging procedures and prognostic features in small cell anaplastic bronchogenic carcinoma. | 1988 | 211639 | |
| inhibition and recovery of dna synthesis in host tissues and sensitive and resistant b16 melanoma after 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea, a predictor of therapeutic efficacy. | single doses of 1-(2-chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea caused transient suppression of [3h]thymidine incorporation into dna in bone marrow and gastrointestinal mucosa and more prolonged inhibition of such incorporation in b16 melanoma. a single dose of 1-(2-chloroethyl) -3- (trans-4-methylcyclohexyl) -1-nitro-sourea, 16 mg/kg, doubled the mean life-span after treatment of c57bl times dba/2f1 male mice bearing 12-day-old b16 melanomas. subsequent doses timed to minimize toxi ... | 1988 | 1149044 |
| [some factors determining the values of spirometric studies in an urban population]. | 1988 | 1135046 | |
| [evaluation of renal cyclic adenosine monophosphate, serum parathyroid hormone and phosphate reabsorption in recurrent calcium urolithiasis, healthy controls and hyperparathyroidism (author's transl)]. | in three groups (n = 12 each) of male controls (22--43 years), patients with recurring calcium urolithiasis (21--36 years) and hyperparathyroidism (hpt; 17--71 years) proven by surgery renal cyclic adenosine monophosphate (rcamp), fractional tubular phosphate reabsorption and serum parathyroid hormone (pth) were measured during endogenous creatinine clearance. rcamp (mumol/g creatinine) was: controls 1.48 +/- sem 0.27; stone formers 2.037 +/- 0.343 (not significantly different); hpt 6.234 +/- 0. ... | 1989 | 210311 |
| radioimmunological determination of free (diffusible) cortisol in an equilibrium dialysis system. | 1989 | 210267 | |
| [studies on retention after chewing fluoride tablets, part 1. residual fluoride amount and rate]. | 1989 | 297708 | |
| effect of ethanol on hepatotoxicity of acetaminophen in mice and on reactive metabolite formation by mouse and human liver microsomes. | the protective effect of a single dose of ethanol with regard to hepatotoxicity caused by acetaminophen (apap) can be a consequence of either direct or indirect inhibition of apap oxidation to its hepatotoxic intermediate (n-acetyl-p-benzoquinoneimine, napqi), or augmentation of repair mechanisms following the hepatotoxic insult. the mechanism of hepatoprotection appears to be species dependent. by varying the time of ethanol administration relative to apap in mice (30 min before to 240 min afte ... | 1989 | 2781565 |
| in-vitro activity of a new penem fce 22101. | the in-vitro activity of a new penem fce 22101 was evaluated in comparison with other antimicrobial agents against 966 aerobic and anaerobic gram-negative and gram-positive bacteria. fce 22101 inhibited 90% of enterobacteriaceae, haemophilus influenzae, h. parainfluenzae, h. ducreyi, neisseria gonorrhoeae, branhamella catarrhalis, staphylococcus aureus, staph. epidermidis and group b streptococcus at concentrations of 0.12-4.0 mg/l. pseudomonas aeruginosa was resistant with mic90 of greater than ... | 1989 | 2786518 |
| changes in aldehyde dehydrogenase during rat urinary bladder carcinogenesis. | we have reported that normal rat urinary bladder possesses significant amounts of an aldehyde dehydrogenase (class 3 aldh) expressed during hepatocarcinogenesis, but not detectable in normal liver. changes in expression of both liver and bladder aldh during n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn)-induced bladder carcinogenesis were studied. the aldh phenotype was determined at intervals over 42 weeks by histochemical analysis, total aldh activity assays and gel electrophoresis using propion ... | 1989 | 2805229 |
| changes in aldehyde dehydrogenase during rat urinary bladder carcinogenesis. | we have reported that normal rat urinary bladder possesses significant amounts of an aldehyde dehydrogenase (class 3 aldh) expressed during hepatocarcinogenesis, but not detectable in normal liver. changes in expression of both liver and bladder aldh during n-butyl-n-(4-hydroxybutyl) nitrosamine (bbn)-induced bladder carcinogenesis were studied. the aldh phenotype was determined at intervals over 42 weeks by histochemical analysis, total aldh activity assays and gel electrophoresis using propion ... | 1989 | 2805229 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| effect of cck antagonists cr 1409 and cr 1505 on rat pancreatic exocrine secretion in vivo. | the action of cholecystokinin (cck) antagonists cr 1409 and cr 1505 on pancreatic exocrine secretion stimulated by exogenous and endogenous cck was studied in vivo in anesthetized rats, and compared with proglumide. intravenous administration of cr 1409 and cr 1505 in graded doses between 0.04 and 25 mg/kg/h resulted in a dose-dependent inhibition in pancreatic juice volume and amylase output stimulated by intravenous infusion of cck-8 in a dose of 0.06 microgram/kg/h. cr 1409 is 1,000 times and ... | 1989 | 2813333 |
| effect of mci-186 on ischemia-induced changes in monoamine metabolism in rat brain. | we examined the effects of mci-186 (3-methyl-1-phenyl-2-pyrazolin-5-one), a novel free radical scavenger and an inhibitor of ischemia-induced brain edema, on monoamine metabolism in the brains of both normal and ischemic rats. in normal rats, 3 mg/kg i.v. mci-186, a dose that prevents ischemic brain edema, had no significant effect on brain concentrations of dopamine, norepinephrine, 5-hydroxytryptamine, or their metabolites. after the injection of 5 microliters of 3% polyvinyl acetate into the ... | 1989 | 2815191 |
| effect of mci-186 on ischemia-induced changes in monoamine metabolism in rat brain. | we examined the effects of mci-186 (3-methyl-1-phenyl-2-pyrazolin-5-one), a novel free radical scavenger and an inhibitor of ischemia-induced brain edema, on monoamine metabolism in the brains of both normal and ischemic rats. in normal rats, 3 mg/kg i.v. mci-186, a dose that prevents ischemic brain edema, had no significant effect on brain concentrations of dopamine, norepinephrine, 5-hydroxytryptamine, or their metabolites. after the injection of 5 microliters of 3% polyvinyl acetate into the ... | 1989 | 2815191 |
| antibiotic irrigation and the formation of intraabdominal adhesions. | the efficacy of antibiotic peritoneal lavage in the prevention of postoperative infection is controversial. the role of intraperitoneally administered cefazolin and tetracycline in the formation of adhesions was studied in the rodent model. thirty-two rats were divided into 3 groups. group 1 underwent midline laparotomy with instillation of 10 ml of normal saline solution. group 2 and group 3 underwent the same procedure with instillation of 0.2 percent saline solutions of cefazolin or tetracycl ... | 1989 | 2817225 |
| [isolation of individual chromosomes and their use in studying the human and animal genome]. | this mini-review considers the main achievements in the field of human and animal chromosome separation: technical problems of flow-karyotyping and flow-sorting, the types of cell lines and chromosome source used, different protocols of chromosome suspension preparation, fluorochromes for chromosome staining, efficiency of chromosome sorting and purity of the fractions. the use of fractions for creating chromosome-specific human gene libraries and mapping cloned genes and dna fragment are also c ... | 1989 | 2685546 |
| [isolation of individual chromosomes and their use in studying the human and animal genome]. | this mini-review considers the main achievements in the field of human and animal chromosome separation: technical problems of flow-karyotyping and flow-sorting, the types of cell lines and chromosome source used, different protocols of chromosome suspension preparation, fluorochromes for chromosome staining, efficiency of chromosome sorting and purity of the fractions. the use of fractions for creating chromosome-specific human gene libraries and mapping cloned genes and dna fragment are also c ... | 1989 | 2685546 |
| [expressions of 2h4 and 4b4 antigens on atl cells]. | we studied the expression of 2h4 and 4b4 on the surfaces of leukemia cells from 17 patients with adult t-cell leukemia (atl) as well as of cells belonging to 2 t-cell lines derived from atl patients. the effects of the supernatants obtained from culture fluids of the atl cells and the t-cell lines on igg production of a human b-cell line, cess cells, were also examined. on the surfaces of the atl cells from 15 out of 17 cases and of the cells of 2 t-cell lines 4b4 obviously existed at higher per ... | 1989 | 2528006 |
| distribution of calcitonin- and somatostatin-containing cells in thyroid lymphoma and in hashimoto's thyroiditis. | eleven cases of thyroid lymphoma were studied by the immunoperoxidase avidin-biotin technique with calcitonin and somatostatin rabbit antisera. in 6 cases of non-hodgkin lymphoma, in thyroid tissue residual to the lymphomatous infiltration, the c cell density was markedly increased and clustering was often observed; the c cells often took part in the follicular lining, frequently with polar distribution; these elements displayed a strong positivity for calcitonin, while the number of somatostati ... | 1989 | 2567176 |
| the effect of pinealectomy and jejunal loop diversion on rat small bowel crypt cell kinetics. | previously, it was found that diversion of an isolated loop of jejunum into the colon was associated with a significantly diminished crypt cell proliferation rate in the isolated loop, probably principally because of the diminished amount of nutrient available to the diverted mucosa. it has also been shown previously that removal of the pineal gland is associated with a considerable increase in the jejunal crypt cell mitotic rate. in the present investigation it was found that following pinealec ... | 1989 | 2570492 |
| functionally different htlv i-infected t cell lines with the same phenotype derived from a patient with melanoma. | two autologous t cell lines infected with htlv i are described. t cells from a patient with malignant melanoma were infected with htlv i by co-culture with a htlv i-producing t cell line, slb i. both t cell lines express identical phenotype (cd3+, cd4+, 4b4+, 2h4-) but they demonstrate marked differences in growth characteristics and function. one of these two lines, referred to as tftx, established from the autologous tumor activated peripheral blood lymphocytes (pbl), grows in culture without ... | 1989 | 2576655 |
| a preliminary survey of parasitic infections and nutritional status among school children in riyadh, saudi arabia. | a total of 320 school children in the model institute of the capital in riyadh, were clinically and parasitologically examined. thirty-five (10.94%) had intestinal parasites. entamoeba coli (61.91%) was found more frequently among the commensals, while giardia lamblia (28.57%) was the most common pathogenic parasite. all urines were parasite free. the low infection rate with gardia could be explained by the good physical and nutritional status and better sanitary and living conditions. | 1989 | 2708849 |
| scanning electron microscopy of very small fat cells and mature fat cells in human obesity. | to determine the effect of obesity on the size distribution of fat cell populations in human adipose tissue, omental fat tissue biopsies were obtained from lean, moderately obese, and massively obese patients. the size distributions of adipocytes from lean and obese fat tissues examined by the scanning electron microscopic method were bimodal, consisting of populations of very small fat cells and mature fat cells, in contrast to collagenase-derived isolated cells that showed only the large matur ... | 1989 | 2715732 |
| purification of alpha-ketoaldehyde dehydrogenase from the human liver and its possible significance in the control of glycation. | alfa-ketoaldehyde dehydrogenase, which was extracted and purified from human livers, may act on carbonyl compounds, such as 3-deoxyglucosone, and be involved in the control of glycation (maillard reaction) in the body. | 1989 | 2721637 |
| regulation of hepatic cortisol sulfotransferase in rats by pituitary growth hormone. | cytosolic sulfating activities of 4-pregnen-11 beta, 17 alpha, 21-triol-3,20-dione (cortisol) to the 21-sulfate were 4 to 5 times higher in livers of female than male adult rats. the activity was decreased by administration of testosterone propionate (tp) to ovariectomized, but not to intact, female rats. in male rats, the rate of cortisol sulfation was elevated by neonatal castration and was restored in part by the administration of tp to the castrated rats. in addition, the sulfating activity ... | 1989 | 2725475 |
| gangrenous ergotism in a suckler herd. | 1989 | 2728284 | |
| internal screw fixation: comparison of placement pattern and rigidity. | multiple screw placement patterns and techniques have been described for fixation of the sagittal split ramus osteotomy. this study evaluated transverse strength (rigidity) of six groups of simulated sagittal osteotomies fixed with different screw patterns and placement techniques. osteotomies fixed with screws placed in an inverted l pattern were significantly more rigid than those fixed with screws placed in a linear pattern or k-wires placed in a triangular pattern (p less than .05). no signi ... | 1989 | 2732831 |
| acquired renal cysts in five-sixths nephrectomized rats: the roles of oxalate deposits in renal tubules and a renotropic factor. | the present study was undertaken to see if the oxalate deposits seen in renal tubules are a causative factor in the development of acquired renal cysts in chronic renal failure. thirty 5/6 nephrectomized rats had free access to water containing 8 mg/ml of vitamin c (oxalate precursor) and 20 5/6 nephrectomized rats were given tap water without vitamin c. oxalate deposits were found on microscopy in the renal tubules of vitamin c-treated rats in the 11th and 12th postnephrectomy months; however, ... | 1989 | 2918951 |
| [the effect of food consistency on conditioned avoidance response in mice and rats]. | the purpose of this study was to examine the effects of chewing a hard and soft diet on acquisition of conditioned avoidance response in mice and rats. 76 c57bl mice and 38 wistar rats were divided into 2 groups respectively 20 days after birth. one group was fed a solid diet and another was fed a powder diet containing the same components as the solid one (nihon crea company, ce-2). using the avoidance conditioning system of gunma university, conditioned avoidance experiments started at the age ... | 1989 | 2519255 |
| diabetic state-induced activation of calcium-activated neutral proteinase in mouse skeletal muscle. | the effect of a diabetic state in the diabetic kk-cay mouse on calcium activated neutral proteinase (canp) of hind-limb skeletal muscles was investigated. in the diabetic state, there was an increased sensitivity to activation of canp by calcium (ca). in addition, there was an enhancement of maximal activity of the enzyme. the effect was induced by secondary modification of the diabetic state, but not genetical factors. several lines of evidence suggest that the canp is responsible for 92 k dalt ... | 1989 | 2561275 |
| changes in oligosaccharides of human blood cells measured by tritium- and fitc-labeled lectins after in vitro- and in vivo-irradiation--lectin binding as a 'biological dosemeter'? | the lectin-binding system has been described previously as a biological dosimeter, by revealing induced changes in oligosaccharides of the cell membrane. the measurements were performed by binding [3h]concanavalin a to blood cells. our results on human blood cells irradiated in vitro with doses in the range 0.5-5 gy indicate great difficulties in using radioactive labeled con-a for an accurate quantitative analysis of radiation effects on cell membranes. it appears nearly impossible to different ... | 1989 | 2565944 |
| development of cellular polarity and tight junctions in parotid acinar cells of postnatal rat. | the morphogenesis of acino-tubular structures and cytodifferentiation of acinar cells in developing rat parotid glands from the day of birth to the 7th day after birth were studied by conventional ultrathin-section electron microscopy in conjunction with freeze replica and space tracer methods. an ultrathin-section study indicated that the acinar cells developed sequentially in the order of the following three stages: (1) the stage of undifferentiated cell immediately after birth, in which the p ... | 1989 | 2584909 |
| in vitro proliferative and cytotoxic responses of pbl from theileria annulata-immune cattle. | peripheral blood lymphocytes were isolated from healthy calves and were subsequently infected with sporozoites of theileria annulata in vitro. the infected cells were passaged for 50 times and thereafter inoculated into animals from which they were previously isolated. within 4-5 days, schizont-containing cells were demonstrable in the lymph nodes of all animals. few days later, merozoites were detected in erythrocytes. a slight decrease in the counts of lymphocytes and leucocytes was also found ... | 1989 | 2596199 |
| activation of human natural killer cells by lipopolysaccharide from actinobacillus actinomycetemcomitans. | lipopolysaccharide (lps) from the y4 strain of this bacterium, which is implicated in the pathogenesis of juvenile periodontitis, was incubated with human peripheral blood lymphocytes (pbl) and its action compared to that of lps from escherichia coli. both lps augmented cytotoxicity measured against natural killer (nk) cell-resistant tumour targets within 24 h of incubation. cytotoxicity was exclusively found in nk-enriched low-density large granular lymphocyte fractions, as separated by percoll ... | 1989 | 2597038 |
| a method for studying rat embryonic metabolism by microcannulation of the vitelline circulation. | a novel technique which involves the cannulation of the vitelline vessels of the 11.5 days rat embryo is described; this has allowed the introduction of both macromolecular and particulate substances directly into the embryo, thus bypassing the digestive and metabolic activity of the visceral yolk sac and facilitating the study of the fate of such substances within the embryo. the method does not affect the growth of the conceptus in vitro providing that inert solutions (e.g. azo blue dye in han ... | 1989 | 2606767 |
| the behaviour of sensory ganglia in relation to their environment. | the nodose ganglion was removed from rats and transplanted into the denervated tongue and into the denervated or innervated sternocleidomastoid muscle. in another procedure the ganglion was sutured to the distal hypoglossal or sciatic nerve. the ganglia failed to develop motor end-plates on denervated muscle. they did, however, induce formation of taste buds on the lingual epithelium. the regenerated nerve fibres ramified extensively within the connective tissue, invading perivascular tissue and ... | 1989 | 2606773 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| high-performance liquid chromatographic determination of pirprofen and five of its metabolites in human plasma without hydrolysis and in human urine before and after chemical hydrolysis. | selective high-performance liquid chromatographic methods for the simultaneous determination of pirprofen and five of its metabolites either in plasma or in urine before and after chemical hydrolysis were developed. after addition of an internal standard and a buffer, the compounds were extracted from plasma using reversed-phase c18 bond-elut columns and from urine using pre-packed silica extrelut 1 columns, back-extraction into sodium hydroxide and acidification of the alkaline phase before inj ... | 1989 | 2613803 |
| the rat 78,000 dalton glucose-regulated protein (grp78) as a precursor for the rat steroidogenesis-activator polypeptide (sap): the sap coding sequence is homologous with the terminal end of grp78. | based on the striking sequence identity between the amino acid sequence of rat steroidogenesis-activator polypeptide (sap) and the carboxyl terminus of the 78,000 dalton glucose-regulated protein (grp78), the precursor-product relationship between grp78 and sap was investigated in leydig cells. immunoblot analysis with peptide antibodies specific for grp78 and sap showed that the putative sap precursor is also immunoreactive with the anti-grp78 antibody. genomic blot hybridizations further revea ... | 1989 | 2628731 |
| long residual activity of bacillus sphaericus 1593 against culex quinquefasciatus larvae in artificial pools. | the microbial larvicide, bacillus sphaericus 1593, was evaluated against culex quinquefasciatus larvae in artificial pools at huay kwang area in bangkok between march and october 1985. this study was aimed at determining the residual activity of b. sphaericus 1593 in waste water under field conditions. the larvicidal activity of b. sphaericus 1593 was found to persist for at least 5 months in artificial pools containing waste water. the populations of b. sphaericus 1593 in the test water fluctua ... | 1989 | 2633350 |
| esculetin inhibits t cell activation without suppressing il-2 production or il-2 receptor expression. | esculetin (6,7-dihydroxycoumarin) was found to inhibit dose-dependently the proliferation of human t cells stimulated by pha or phorbolester plus ionomycin. proliferation in autologous and allogeneic mlr and generation of cytotoxic t cells under limiting dilution conditions were also suppressed, with more than 90% inhibition seen at 50 microm esculetin. the immunosuppressive effect of esculetin was not due to toxicity. esculetin did not inhibit interleukin-2 (il-2) production, nor did it interfe ... | 1989 | 2654016 |
| nasal obstruction and human communication. | nasal obstruction may cause a variety of communication disorders, particularly in children. the effects of nasal obstruction on hearing, speech, language, and voice are examined. methods for assessing the effects of nasal obstruction are delineated, and recommendations for therapeutic interventions are described. | 1989 | 2664661 |
| granule core loss in eosinophils from a patient with aspirin-induced asthma: an electron microscopic study. | aspirin and other antiinflammatory drugs except steroid hormones precipitate asthmatic attacks in some patients with nasal polyps. the respiratory reaction can be severe and life-threatening. the incidence of nasal polyp found in aspirin-induced asthma ranges from 60% to 100%. this case report concerns a 27-year-old japanese man who has suffered from asthma for 4 years and had attacks of asthma after ingestion of antipyretics. he had nasal polyps and sinusitis. the polyps were removed and examin ... | 1989 | 2802266 |
| formation of the 1-(s-glutathionyl)-2,4,6-trinitrocyclohexadienate anion at the active site of glutathione s-transferase: evidence for enzymic stabilization of sigma-complex intermediates in nucleophilic aromatic substitution reactions. | formation of the meisenheimer complex or sigma-complex [1-(s-glutathionyl)-2,4,6-trinitrocyclohexadienate] between glutathione (gsh) and 1,3,5-trinitrobenzene (tnb) can be observed at the active sites of isoenzymes 3-3 and 4-4 of rat liver gsh transferase. the spectroscopic properties (uv-visible and cd) of the enzyme-bound sigma-complex are consistent with a 1:1 complex in an asymmetric environment. competitive inhibitors which occupy the gsh binding site (e.g., gamma-l-glutamyl-d,l-2-aminomalo ... | 1989 | 2789999 |
| combined gastric pull-up and microvascular jejunal transfer procedure after pharyngolaryngoesophagectomy. | a case of simultaneous carcinomas of the lower thoracic esophagus and the oropharynx in a 62-year-old man is reported. reconstruction was successfully performed using a one-stage operation consisting of pharyngolaryngoesophagectomy followed by a combination of gastric pull-up and microvascular jejunal transfer procedures. the postoperative course was uneventful except for regurgitation of food, which persisted for 1 month. | 1989 | 2774731 |
| axonal signals regulate expression of glia maturation factor-beta in schwann cells: an immunohistochemical study of injured sciatic nerves and cultured schwann cells. | glia maturation factor-beta (gmf-beta) is a 17 kda protein purified and sequenced from bovine brains. using the monoclonal antibody g2-09 directed against gmf-beta, we previously demonstrated endogenous gmf-beta in astroblasts, schwann cells, and their tumors in culture. in the present study, we have used indirect immunofluorescence microscopy with g2-09 to examine the effects of transection, crush, and regeneration of sciatic nerve on the expression of gmf-beta in schwann cells in situ and to s ... | 1989 | 2795149 |
| mechanism of action of human pancreatic and salivary alpha-amylase on 4,6-ethylidene-alpha-4-nitrophenyl-maltoheptaoside substrate. | 1989 | 2783397 | |
| mechanism of action of human pancreatic and salivary alpha-amylase on 4,6-ethylidene-alpha-4-nitrophenyl-maltoheptaoside substrate. | 1989 | 2783397 | |
| transformation of oocysts from several coccidian species by heat treatment. | the transformation of sporocysts in oocysts of eimeria caviae, e. intestinalis, e. nieschulzi, e. separata, e. tenella, isospora canis, i. heydorni, and toxoplasma gondii by heat treatment was examined. fresh unsporulated oocysts from these species were heated at 50 degrees or 55 degrees c for less than 5 min and cultivated at 25 degrees c for 1 week. no transformation of sporocysts was observed in the sporulated oocysts following heating at 55 degrees c, but sporulated oocysts of tyzzeria and c ... | 1989 | 2704721 |
| comparative study on breast milk of mothers delivering preterm and term infants--protein, fat and lactose. | milk samples were collected from 35 mothers delivering preterm infants and from 35 mothers delivering term infants at 3 time intervals over the first 3 weeks of lactation. the samples were collected by manual expression from both breasts into polyethylene containers and were frozen at -20 degrees c and stored for analysis. the protein content of preterm milks during the first and second weeks is higher than that of term milk (p less than 0.05). the fat and lactose contents during the first week ... | 1989 | 2725632 |
| properties and incompatibility behavior of miniplasmids derived from the bireplicon plasmid pcg86. | many plasmids belonging to the f incompatibility groups contain more than one basic replicon. the chimeric plasmid pcg86 is an example of such a multireplicon plasmid. the two basic replicons of pcg86, repfiia/fic and repfib have been cloned and re-ligated, the copy numbers of the clones have been determined, and the incompatibility behavior of plasmids containing the ligated replicons and the individual replicons has been studied. the bireplicon plasmids are not expected to be incompatible as r ... | 1989 | 2674652 |