Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [phototransformations of bacteriopheophytin in reaction centers of rhodospirillum rubrum and crhomatium minutissimum]. | 1976 | 817883 | |
| triplet states of bacteriochlorophyll and carotenoids in chromatophores of photosynthetic bacteria. | chromatophores from photosynthetic bacteria were excited with flashes lasting approx. 15 ns. transient optical absorbance changes not associated with the photochemical electron-transfer reactions were interpreted as reflecting the conversion of bacteriochlorophyll or carotenoids into triplet states. triplet states of various carotenoids were detected in five strains of bacteria; triplet states of bacteriochlorophyll, in two strains that lack carotenoids. triplet states of antenna pigments could ... | 1976 | 823977 |
| molecular and catalytic properties of ribulose 1,5-bisphosphate carboxylase from the photosynthetic extreme halophile ectothiorhodospira halophila. | d-ribulose 1,5-bisphosphate (rubp) carboxylase has been purified from the photosynthetic extreme halophile ectothiorhodospira halophila. despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of rubp carboxylase obtained from this organism were inhibited by salts. sedimentation equilibrium analyses showed the enzyme to be large (molecular weight: 601,000). the protein was composed of two types of polypeptide chains of 56,000 and ... | 1976 | 947890 |
| cysteine and s-sulfocysteine biosynthesis in phototrophic bacteria. | forteen species (17 strains) of phototrophic bacteria as well as one strain of thiobacillus denitrificans were tested for cysteine synthase and s-sulfocysteine synthase. all strains contain cysteine sythase active with o-acetylserine; only the chromatiaceae, two species of the rhodospirillaceae and t. denitrificans contain s-sulfocysteine synthase. in six species repression by different sulfur compounds in the medium was studied. in chromatium vinosum, cysteine synthase was found to be constitut ... | 1976 | 962465 |
| [oxidation-reduction potentials of chlorophyll pigments in photosynthesizing organisms on different levels of evolutionary development]. | according to polarographically measured eored of bacteriochlorophyll "a", chlorobium chlorophyll "660", "b" and "a" chlorophylls (-0.67 v, -0.73 v, -0.79 v, -0.86 v-n.h.e.) and literature data about eoox of these pigments (+/-0.62 v, + 0.62 v, + 0.78 v, +0.77 v correspondingly) it has been shown that chemical energy stored in the singular photochemical set is increased in the evolution of the photosynthetic apparatus. it has apparently resulted in the possibility of evolutionary approach to util ... | 1976 | 963091 |
| isolation and characterization of the lipopolysaccharide of chromatium vinosum. | lipolysaccharide was isolated from chromatium vinosum by phenol/water extraction. the lipopolysaccharide is found exclusively in the phenol phase and can be cleaved into a sugar moiety and a lipid a fraction by hydrolysis in 10% acetic acid at 100 degrees c for 3-4 h. the sugar moiety contains the neutral sugars 3-o-methyl-d-ribose, d-ribose, l-arabinose, mannosamine and glucose, and smaller quantities of d-rhamnose, d-glycero-d-manno-heptose (tentatively identified), quinovosamine and 2-keto-3- ... | 1976 | 976262 |
| [growth of purple sulfur bacteria in the dark in anaerobic conditions]. | phototrophic sulphur bacteria chromatium minutissimum 1 c, ectothiorhodospira shaposhnikovii 1, and various strains of thiocapsa roseopersicina grow in the dark under anaerobic conditions (hydrogen or argon) on organic media in the presence of sulphide, thiosulphate or molecular sulphur. ect. shaposhnikovii grows also in the presence of sulphite, cysteine, methionine, glutathione, and, to a less degree, sulphates. cultures of ect. shaposhnikovii growing in the dark under anaerobic conditions ass ... | 1976 | 979675 |
| chemical modification by trinitrobenzenesulfonate of a lipid and proteins of intracytoplasmic membranes isolated from chromatium vinosum and azotobacter vinelandii. | 1. the structure of intracytoplasmic membranes of a photosynthetic bacterium chromatium vinosum and a nitrogen-fixing bacterium azotobacter vinelandii was studied by chemical modification of amino groups of phosphatidylethanolamine and proteins with trinitrobenzenesulfonate. 2. almost all the constituents of intracytoplasmic membranes of c. vinosum were solubilized in a mixture of chloroform, methanol and trichloroacetic acid. one-third of proteins in the intracytoplasmic membranes of c. vinosum ... | 1976 | 999930 |
| photoreduction of the long wavelength bacteriopheophytin in reaction centers and chromatophores of the photosynthetic bacterium chromatium vinosum. | 1976 | 1001464 | |
| [bacteriochlorophyll fluorescence changes related to the bacteriopheophytin photoreduction in the chromatophores of purple sulfur bacteria]. | it is shown that illumination of chromatophores of sulfur bacterium chromatium minutissimum at eh of the medium --200 mv divided by --620 mv (when the photooxidation of pigment p890 is completely inhibited) induces a decrease in bacteriochlorophyll fluorescence yield, reversible in the dark. under these conditions a reversible photoreduction of bacteriopheophytin is detected (bleaching of absorption bands at 543 and 760 nm and development of a band at 650 nm), which is accompanied by a blue shif ... | 1976 | 1024595 |
| coupling factors atpases from photosynthetic bacteria. | 1976 | 134033 | |
| electron transfer in the photosynthetic reaction center. | 1976 | 222400 | |
| kinetic studies of the oxidation and reduction of chromatium high potential iron-sulfur protein (hipip) by inorganic complexes. comparison of the electron transfer reactivities of hipip and horse heart cytochrome c. | 1976 | 176192 | |
| oxidation-reduction properties of several low potential iron-sulfur proteins and of methylviologen. | apparent oxidation-reduction potentials at ph 7.0 and 25 degrees c were determined using the h2-hydrogenase system with ferredoxins from the following sources: clostridium pasteurianum, -403 mv; c tartarovorum, -424 mv; c. acidi-urici, -434 mv; peptococcus aerogenes, -427 mv; chromatium d, -482 mv (ph 8.0); b. polymyxa, fd i, -377 mv, and fd ii, -422 mv; and spinach, -428 mv. the ph dependence of these values was variable, ranging from -2 to -24 mv/ph unit increase for different ferredoxins. ove ... | 1976 | 181047 |
| visible absorption spectra of quantum mixed-spin ferric heme proteins. | recently, it has been shown that the magnetic data for chromatium ferricytochrome c' at ph 7 are consistent with quantum mechanically (as distinguished from thermally) mixed mid-spin (s = 3/2) and high-spin (s = 5/2) heme. visible absorption spectra of the protein measured at 77 degrees k and 293 degrees k, ph 7, show peaks at 400, 490, and 632 nm. the observation of a 630 nm band in quantum mixed-spin heme spectra, and the spin state-dependence of the band intensity, are discussed in the contex ... | 1976 | 182223 |
| epr properties of the electron carrier intermediate between the reaction center bacteriochlorophylls and the primary acceptor in chromatium vinosum. | 1976 | 182533 | |
| the primary photoreactions in the complex cytochrome-p-890-p-760 (bacteriopheophytin760) of chromatium minutissimum at low redox potentials. | experimental evidence for electron transfer, photosensitized by bacteriochlorophyll, from cytochrome c to a pigment complex p-760 (involving bacteriopheophytin-760 and also bacteriochlorophyll-800) in the reaction centers of chromatium minutissimum has been described. this photoreaction occurs between 77 and 293 degrees k at a redox potential of the medium between -250 and -530 mv. photoreduction of p-760 is accompanied by development of a wide absorption band at 650 nm and of an epr signal with ... | 1976 | 183814 |
| epr and optical spectroscopic properties of the electron carrier intermediate between the reaction center bacteriochlorophylls and the primary acceptor in chromatium vinosum. | 1. a reaction center-cytochrome c complex has been isolated from chromatium vinosum which is capable of normal photochemistry and light-activated rapid cytochrome c553 and c555 oxidation, but which has no antenna bacteriochlorophyll. as is found in whole cells, ferrocytochrome c553 is oxidized irreversibly in milliseconds by light at 7 k. 2. room temperature redox potentiometry in combination with epr analysis at 7 k, of cytochrome c553 and the reaction center bacteriochlorophyll dimer (bchl)2 a ... | 1976 | 187221 |
| the primary acceptor of bacterial photosynthesis: its operating midpoint potential? | 1976 | 4013 | |
| mechanism of inhibition of chromatium d growth by l-methionine. regulation of l-threonine biosynthesis by the intracellular level of s-adenosylmethionine. | (1) an unusual accumulation of s-adenosyl-l-methionine in chromatium d was associated with a marked growth inhibition by l-methionine. the inhibition was overcome by l-isoleucine, l-leucine, l-phyenylalanine, l-threonine, l-valine and putrescien. based on their effects, these compounds are classified into 3 types. (2) l-isoleucine, l-leucine, l-phyenylalanine and l-valine (type i) inhibited the l-methionine uptake and consequently prevented the bacterium from the unusual accumulation of s-adenos ... | 1976 | 8102 |
| on the ecology of marine chromatiaceae and chlorobiaceae (author's transl). | number and genera (types) of phototrophic red and green sulfur bacteria have been determined in the upper layer of eleven marine sediment samples from the euphotic zone in the mediterranean sea. origin and nature of the sediment samples are given as well as their ph, total nitrogen and sulfide content. the results show that the sediments studied did not provide good growth conditions for chromatiaceae (chromatium, thiocystis, thiocapsa) and chlorobiaceae (prosthecochloris). the total number of t ... | 1976 | 9896 |
| magnetic studies of chromatium flavocytochrome c552. a mechanism for heme-flavin interaction. | electron paramagnetic resonance and magnetic susceptibility studies of chromatium flavocytochrome c552 and its diheme flavin-free subunit at temperatures below 45 degrees k are reported. the results show that in the intact protein and the subunit the two low-spin (s = 1/2) heme irons are distinguishable, giving rise to separate epr signals. in the intact protein only, one of the heme irons exists in two different low spin environments in the ph range 5.5 to 10.5, while the other remains in a con ... | 1976 | 9997 |
| fermentation of pyruvate by 7 species of phototrophic purple bacteria. | the dark, anaerobic fermentation of pyruvate under growth conditions was examined with the following species of phototrophic purple bacteria: rhodospirillum rubrum strains ha and s1, rhodopseudomonas gelatinosa strain 2150, rhodopseudomonas acidophila strain 7050, rhodopseudomonas palustris strain atcc 17001, rhodopseudomonas capsulata strains kb1 and 6950, rhodopseudomonas sphaeroides strain atcc 17023, and chromatium vinosum strain d. fermentation balances were established for all experiments. ... | 1976 | 12621 |
| proceedings: changes in the physical parameters of photosynthetic membranes associated with energy transduction-some temperature-sensitive processes. | 1976 | 1084343 | |
| oxidation-reduction properties of chromatium vinosum high potential iron-sulfur protein. | the oxidation-reduction properties of the high potential iron-sulfur protein (hipip) from chromatium vinosum have been investigated. both equilibrium and kinetic measurements demonstrate electron transport by hipip is ph independent in the ph range 7-11. the kinetics of reduction (potassium ferrocyanide, so2, s2o42-, sodium ascorbate, and rhodospirillum rubrum cytochrome c2) and oxidation (potassium ferricyanide and rhodospirillium rubrum cytochrome c2) of hipip are reported. based on the data o ... | 1976 | 2285 |
| microencapsulation of chloroplast particles. | chloroplast and photosystem i particles were encapsulated in small spheres (about 20 mum diameter) with an artificial membrane built up by cross-linking amino groups of protamine with toluenediisocyanate. the artificial membrane was permeable to small substrate and product molecules but not to soluble proteins. photosystem i activity was retained by the encapsulated chloroplast particles. washed photosystem i particles were encapsulated with the soluble proteins, ferredoxin, and ferredoxin-nadp ... | 1976 | 16659564 |
| light-induced generation of electric potential difference in membranes of purple and green sulfur bacteria. | when associated with a planar phospholipid membrane, chromatophores isolated from photosynthetic sulfur bacteria chromatium minutissimum, ectothiorhodospira shaposhnikovii, and chlorobium limicola f. thiosulfatophilum were shown to generate a light-induced transmembrane electric potential difference measured by a direct method using macroelectrodes and a voltmeter. the maximal photoelectric responses were observed upon the addition of 1,4-naphthoquinone in combination with phenazine methosulfate ... | 1977 | 18265514 |
| delayed fluorescence from bacteriochlorophyll in chromatium vinosum chromatophores. | delayed fluorescence from bacteriochlorophyll in chromatium vinosum chromatophores was studied at room temperature and under intermittent illuminations. the decay of delayed fluorescence was constituted of two components; a fast component decayed with a half time of about 8 ms, a slow one decayed in parallel with the reduction of photooxidized bacteriochlorophyll (p+) with a half time of 100-200 ms. the biphasic decay of delayed fluorescence indicated that a rapid equilibrium was established bet ... | 1977 | 12813 |
| kinetic study of the dissolution of fe4s4(2-)-cluster core ions of ferredoxins and high potential iron protein. | 1977 | 18167 | |
| utilization of nitrogen compounds and ammonia assimilation by chromatiaceae. | chromatium vinosum strain d, thiocapsa roseopersicina strain 6311 and ectothiorhodospira mobilis strain 8112 were grown anaerobically in the light with various single nitrogen sources. when substituted for nh4cl only glutamine and casamino acids supported good growth of all strains tested. peptone and urea were utilized by c. vinosum and t. roseopersicina, glutamate, asparagine and nitrate only by c. vinosum. the strains were able to grow with molecular nitrogen; complete inhibition of this grow ... | 1977 | 19002 |
| dependence of the rates of dissolution of the fe4s4 clusters of chromatium vinosum high-potential iron protein and ferredoxin on cluster oxidation state. | the influence of oxidation state on the ph dependence of the dissolution of the fe(4)s(4) clusters of chromatium vinosum ferredoxin and high-potential iron protein (hipip) has been studied. the first-order rate constants (k(obs)) for dissolution of both the fe(4)s(4)(s-cys)(4) (2-) and fe(4)s(4)(s-cys)(4) (3-) clusters of the ferredoxin follow the same overall kinetic equation but with differing specific rate and equilibrium constants. the dependence of rate and equilibrium constants upon oxidat ... | 1977 | 23530 |
| near-infrared magnetic circular dichroism of cytochrome c'. | the near-infrared magnetic circular dichroism (mcd) of rhodospirillum rubrum, chromatium vinosum, and rhodopseudomonas palustris cytochromes c' are reported. the spectra of the reduced protein are very similar to those of deoxymyoglobin. the spectra of the oxidized proteins in the pd range 1-13 can be analyzed on the basis of four species a, b, c, and d. the existence of nine species, reported in a recent electron paramagnetic resonance study, is not substantiated. the mcd spectra support the as ... | 1977 | 192272 |
| enzymic formation of glycolate in chromatium. role of superoxide radical in a transketolase-type mechanism. | chromatophores prepared from chromatium exhibit a light-dependent o2 uptake in the presence of reduced 2,6-dichlorophenolindophenol, the maximum rate observed being 10.8 micronmol (mg of bchl)-1 h-1 (air-saturated condition). as it was found that the uptake of o2 was markedly inhibited by superoxide dismutase, it is suggested that molecular oxygen is subject to light-dependent monovalent reduction, resulting in the formation of the superoxide anion radical (o2-). by coupling baker's yeast transk ... | 1977 | 193557 |
| evidence for a thioether linkage between the flavin and polypeptide chain of chromatium cytochrome c 552. | 1977 | 194892 | |
| electron-transfer protein reactivities. kinetic studies of the oxidation of horse heart cytochrome c, chromatium vinosum high potential iron-sulfur protein, pseudomonas aeruginosa azurin, bean plastocyanin, and rhus vernicifera stellacyanin by pentaamminepyridineruthenium(iii). | 1977 | 194940 | |
| function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of chromatium vinosum as studied with flash spectroscopy. | 1977 | 196641 | |
| electron paramagnetic resonance determination of a low-lying excited state in chromatium vinosum high-potential iron protein. | the temperature dependence of the epr spectrum of oxidized high-potential iron protein from chromatium vinosum has been studied. from line width and intensity measurements it is possible to determine the position of the first excited unoccupied state, 160 +/- 10 cm-1 above the ground state orbital. | 1977 | 198036 |
| properties of atpase activity in coupling factor from chromatium strain d chromatophores. | coupling factor extracted from chromatophores of the photosynthetic bacteria chromatium strain d was partially purified. the enzyme catalyzed atpase activity in the presence of ca2+ and mg2+ ions. higher vapp values were obtained when the activity was measured as a function of the divalent cation-atp complex rather than as a function of either the divalent cation or atp because the free components competitively inhibited the activity in the presence of the cation-atp complex. the km values were ... | 1977 | 140048 |
| chromatium hydrogenase. | previous reports of a relatively air-stable soluble hydrogenase from the photosynthetic anaerobe, chromatium vinosum, have been substantiated. the properties of this enzyme, as seen in highly purified samples prepared by procedures that permit improved yields, are described. a possible role for flavin mediation is noted. | 1977 | 265580 |
| formation of cobalt-free corrinoids in rhodopseudomonas spheroides. | co-free corrinoids (cfcs) were found in the cells of rhodopseudomonas spheroides grown without cobalt salt. a predominant fraction of three cfcs on paper-electrophoregram was further purified, and investigated physico-chemically as well as microbiologically in comparison with that isolated by toohey from chromatium. the electrophoretic as well as spectrophotometric patterns of cfc from rh. spheroides were partly different from those of chromatium cfc. the former cfc was a competitive inhibitor o ... | 1977 | 335029 |
| [oxygen effect in photosynthetic organisms (author's transl)]. | 1977 | 337402 | |
| mechanisms of co2 fixation in bacterial photosynthesis studied by the carbon isotope fractionation technique. | the carbon isotope discrimination properties of a representative of each of the three types of photosynthetic bacteria chlorobium thiosulfatophilum, rhodospirillum rubrum and chromatium and of the c3-alga chlamydomonas reinhardii were determined by measuring the ratio of 13co2 to 12co2 incorporated during photoautotrophic growth. 2. chromatium and r. rubrum had isotope selection properties similar to those of c3-plants, whereas chlorobium was significantly different. 3. the results suggest that ... | 1977 | 402896 |
| reduction of adenylylsulfate and 3'-phosphoadenylylsulfate in phototrophic bacteria. | extracts of 14 species of phototrophic bacteria, partly grown with different sulfur compounds, were tested for their ability to form volatile sulfur compounds from adenylylsulfate (aps) and 3'-phosphoadenylylsulfate (paps). the rhodospirillum species showed marked activities with both aps and paps while the rhodopseudomonas species seem to prefer paps. the chromatiaceae exhibited the strongest activities with aps, whereas chlorobium limicola had equally high activity with paps. | 1977 | 408177 |
| [carbonic anhydrase activity of phototropic bacteria]. | the activity of carboanhydrase was assayed in seven species of phototrophous bacteria: three species of the rhodospirillaceae genus, three species of the chromatiaceae genus, and one species of the chlorobiaceae genus. the activity of carboanhydrase was found in five species among seven. it decreased on passing from photoautotrophous to photoheterotrophous conditions of growth, and then to dark heterotrophous conditions, which correlated with the activity of the calvin cycle in the bacteria. no ... | 1977 | 408582 |
| determination of glycolic acid by the eegriwe (calkins) methods. | 1977 | 414636 | |
| studies on the surface structure of the intracytoplasmic membrane in the photosynthetic purple bacterium chromatium vinosum by means of chemical modification. | by means of a chemical modification technique, the surface structure of the intracytoplasmic membrane (chromatophores) of the photosynthetic bacterium chromatium vinosum was investigated. trinitrobenzenesulfonate was employed to modify the primary amino groups of phosphatidylethanolamine and proteins. the experimental results indicate that upon the chemical modification of intact cells, the amino groups on the inner surface of the chromatophores are trinitrophenylated, while upon the modificatio ... | 1977 | 591499 |
| nmr characterization of three forms of ferredoxin from desulphovibrio gigas, a sulphate reducer. | a nmr and magnetic susceptibility study of the oxidized and reduced states of three different oligomers (forms) of a [4fe-4s] ferrodoxin protein from desulphovibrio gigas, fdi, fdi', and fdii was carried out. fdi and fdi' are different trimers and fdii a tetramer of the same basic subunit. a probable assignment of the contact shifted resonances is indicated. since the temperature dependences of the contact shifted responances associated with each [4fe-4s] are not all similar a delocalized model ... | 1977 | 836818 |
| ribosomal ribonucleic acid cistron homologies among hyphomicrobium and various other bacteria. | the extent of hybrid formation between the ribosomal ribonucleic acid (r-rna) of hyphomicrobium strain b-522 and deoxyribonucleic acid (dna) from bacteria of 21 different genera was examined. three generalized groupings were formed. group i (72-100%) consisted entirely of other strains of hyphomicrobium. representatives of the genera rhodopseudomonas, chromatium, caulobacter, prosthecomicrobium, rhodomicrobium, hyphomonas, and hyphomicrobium made up group ii (49-69%). the remaining gram-negative ... | 1977 | 861853 |
| [photosynthetic development of purple sulfur bacteria during illumination with green light]. | the photosynthetic purple sulphur bacterium chr. vinosum grows by assimilating carbon dioxide at the account of the energy of light of different spectral composition. short wavelengths of physiological radiation, blue and green, the region of carotenoid absorption, as well as white light, are used by the bacterium for assimilation of carbon dioxide, biosynthesis of biomass, protein, and pigments. therefore, the possibility of utilization of the energy of green light for bacterial photosynthesis ... | 1977 | 870803 |
| [comparative study of the molecular organization of light-collecting complexes of chromatium minutissimum and rhodopseudomonas palustris]. | 1977 | 872730 | |
| effect of reduction of the reaction center intermediate upon the picosecond oxidation reaction of the bacteriochlorophyll dimer in chromatium vinosum and rhodo pseudomonas viridis. | 1977 | 880297 | |
| delayed fluorescence from bacteriochlorophyll in chromatium vinosum chromatophores: characteristics in the presence of o-phenanthroline. | delayed fluorescence from bacteriochlorophyll in the chromatophores of chromatium vinosum, a photosynthetic purple sulfur bacterium, was studied in the presence of o-phenanthroline (o-phen) under intermittent illumination. re-reduction of the photooxidized reaction center bacteriochlorophyll (p+) in the dark interval was accelerated by o-phen. this effect was attributed to the return of electrons trapped in the primary electron acceptor (a) to p+. in the presence of o-phen, the time course of th ... | 1977 | 881414 |
| [carbohydrate metabolism enzymes of purple sulfur-bacteria during growth in the dark]. | ectothiorhodospira shaposhnikovii, chromatium minutissimum and thiocapsa roseopersicina were grown in the dark under anaerobic conditions on media containing glucose or fructose and organic acids. their cell contained the following enzymes of the fructose diphosphate pathway: phosphofructokinase, fructose diphosphate aldolase, and 3-phosphoglyceraldehyde dehydrogenase. the activity of fructose diphosphate aldolase was higher in the cells grown in the dark than in the cells grown in the light. th ... | 1977 | 882007 |
| biological and physicochemical properties of the lipopolysaccharide of chromatium vinosum. | the lipopolysaccharide (lps) of chromatium vinosum has anticomplementary activity. this anticomplementary activity is destroyed by alkaline digestion of the lps and is suppressed by both mg2+ and ca2+ ions. treatment of the lps with ethylenediaminetetraacetic acid, sodium deoxycholate, or dimethyl sulfoxide did not affect its toxicity toward mice; however, alkaline-treated lps was not toxic. treatment of the lps with sodium deoxycholate, dimethyl sulfoxide, or sodium dodecyl sulfate resulted in ... | 1977 | 892903 |
| variation in guanosine 5' ,3'-tetraphosphate levels in chromatium. | chromatium produces guanosine 5' ,3'-tetraphosphate, but there appears to be no correlation between guanosine 5' ,3'-tetraphosphate concentration and the inability of the organism to grow in certain light-dark regimes. | 1977 | 893339 |
| amino acid sequence of chromatium vinosum ferredoxin: revisions. | reexamination of the amino acid sequence of chromatium vinosum ferredoxin revealed that the original sequence proposed in 1970 (i) should be revised. two segments in the sequence, residues 50-52 and residues 53-57, should be mutually displaced and therefore, the correct sequence in this region is concluded to be -val-glu-val-cys-pro-val-asp-cys-(residues 50-57). another correction was the addition of one isoleucine residue between residues 57 and 58 and therefore, the total number of residues sh ... | 1977 | 893371 |
| [concentration of polyvalent metals following a change in the metabolism of chromatium vinosum]. | the composition of polyvalent metals (fe, mn, cu, ti, ni, co, v, cr) was compared during photoheterotrophous and autotrophous growth of chromatium vinosum. a comparatively high content of mn and cr, i. e. metals whose amount increases in the course of evolution of photoautotrophous organisms, is typical of the bacterium after autotrophous growth. an increase in the concentration of ti, i. e. a metal involved in the reduction reactions of the cells, is characteristic of photoheterotrophous growth ... | 1977 | 895552 |
| purification and photochemical properties of reaction centers of chromatium vinosum. evidence for the photoreduction of a naphthoquinone. | 1977 | 901774 | |
| [possibility of using light of different wavelengths for growing chromatium vinosum in heterotrophic conditions]. | the photoautotrophic purple sulphur bacterium chronatium vinosum was found to be capable of heterotrophic growth under anaerobic conditions at the expense of light energy. no growth was detected under anaerobic conditions in the absence of light. the energy of monochromatic red light (715, 726, 739, 776, 815 nm) and green light (534 and 535 nm), as well as of green luminescent light (a broad maximum at 500-560 nm, a peak at 520 nm) and white light, was used by the bacterium for the growth under ... | 1977 | 909462 |
| new stereochemical analogies between iron-sulfur electron transport proteins. | active sites of chromatium high potential iron protein (hipip) and pseudomonas aerogenes ferredoxin can be brought into equivalent orientations by assuming that their fe4s4sgamma4 clusters have the effective symmetry of the non-axial molecular point group cs. previously undetected analogies between the two proteins emerge as a result of selecting a common orientation in this mammer. polypeptide segments connecting cys 46 to cys 63 in hipip and cys 18 to cys 35 in ferredoxin are analogous in the ... | 1977 | 914840 |
| [comparative study of light-harvesting complexes of purple photosynthetic bacteria chromatium minutissimum and rhodopseudomonas palustris]. | light-harvesting pigment-lipoprotein complexes from sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple bacteria are isolated and comparatively studied. electron microscopy was used for determination of the complex size, different methods were employed to estimate their molecular weights and chemical composition. two small proteins are found in each complex, their molecular weight, molar ratio and their content per complex being studied. amino acid composition and ... | 1977 | 922068 |
| on the mechanism of glycolate synthesis by chromatium and chlorella. | 1978 | 623491 | |
| superoxide dismutase from an anaerobic photosynthetic bacterium, chromatium vinosum. | 1978 | 626504 | |
| the effects of uncoupler on the rates of cytochrome oxidation and reduction in the photosynthetic bacterium, chromatium. evidence for a possible cytochrome switching. | the uncoupler, m-chlorocarbonyl cyanide phenylhydrazone (cccp) added to anerobic, dark-adapted whole cells of chromatium vinosum is found to speed the reduction of cytochrome after oxidation by laser or by steady illumination and, subject to unknown factors, to slow the laser-induced oxidation. there is considerable evidence, including spectra and loss of low-temperature oxidizability that this results from a switch of the identity of the cytochrome oxidized from the low-potential cytochrome c-5 ... | 1978 | 629959 |
| [study of the state of intracellular iron in photosynthesizing purple sulfur bacteria using the mossbauer effect]. | the work presents the results of the first stage of the study on the valent and structural state and function of the iron atoms in the donor-acceptor environment of the photosynthetic reaction centres of purple bacteria. 57fe was introduced by cultivating the microorganisms in a medium enriched in this isotope. at 77 k the maxima observed in the mössbauer spectra in intact freeze-dried cells at a speed of +2 mm/s and --1 mm/s are attributed to doublets 1.11 with the isomer ahifts of 1.3 and 0.5 ... | 1978 | 634286 |
| amino acid sequence of a ferredoxin from chlorobium thiosulfatophilum strain tassajara, a photosynthetic green sulfur bacterium. | we have determined the amino acid sequence of a ferredoxin from a photosynthetic green sulfur bacterium, chlorobium thiosulfatophilum strain tassajara. it contains 61 amino acid residues with 9 cysteines, and 8 of the 9 were located at positions corresponding to those in clostridial-type ferredoxins. other structural features were closer to those of ferredoxins from another photosynthetic bacterium, c. limicola, than to those of non-photosynthetic bacteria. compared with ferredoxin from chromati ... | 1978 | 659399 |
| [polarographic study of chlorophyll in aqueous solutions of detergents and in the composition of a chlorophyll-protein complex]. | 1978 | 667146 | |
| pseudomonas ovalis ferredoxin: similarity to azotobacter and chromatium ferredoxins. | 1978 | 680138 | |
| [molecular organization of the long-wave complexes of purple photosynthesizing bacteria. effect of pronase on the b890 complex of chromatium minutissium and rhodopseudomonas palustris]. | the pronase action on the long-wave complexes b890 from two different purple bacteria has been investigated. differences in the kinetics of decrease of the reaction center photochemical activity of electron-donor activity of cytochromes and of destruction of bx890 (875) forms have been discovered. different rates of the proteolysis of rc proteins were revealed by sds-gel-electrophoresis. the heavy protein of rc was the first to degrade. the photochemical transformations deltaa890(875) in b890 co ... | 1978 | 683188 |
| isolation and characterization of the lipopolysaccharide of thiocapsa roseopersicina. | the lipopolysaccharide from thiocapsa roseopersicina was isolated by phenol/water, being found in the water phase. it is cleaved into a polysaccharide moiety (degraded polysaccharide) and lipid a by hydrolysis with 10% acetic acid (100 degree c, 3 h). d-mannose, l-rhamnose, 3-amino-3, 6-dideoxy-d-galactose and d-glucose are the major constituents of the degraded polysaccharide. 2-o-methyl-l-rhamnose, 3-o-methyl-d-mannose, d-galactose, glucosamine and quinovosamine are minor constituents. d-glyce ... | 1978 | 710428 |
| oxygen-stable hydrogenase and assay. | 1978 | 713840 | |
| purification of (4fe-4s)1--2--ferredoxins (high-potential iron--sulfur proteins) from bacteria. | 1978 | 713842 | |
| [change in the concentration of rna in the cytoplasm of cells of the inferior colliculus in cats during monaural and binaural stimulation]. | the method of cytospectrophotometry of the gallocyanin-chromatium stained sections was used to show that the 2-hour monaural and 1.5-hour binaural stimulation with rhythmical noise signals result in a pronounced increase in cytoplasmatic rna content per cell in the principal and large multipolar neurons. the increase in cytoplasmatic rna amount in the principal cells both from ipsi- and contralateral central nuclei of the inferior colliculus with monaural and binaural stimulations allows to sugg ... | 1978 | 724002 |
| circular dichroism and magnetic circular dichroism of iron-sulfur proteins. | circular dichroism (cd) and magnetic circular dichroism (mcd) spectra are reported for the 2-fe ferredoxins from pseudomonas putida and spirulina maxima, chromatium hipip, the 4-fe ferredoxin from bacillus stearothermophilus, and the 8-fe ferredoxin from clostridium pasteurianum. the spectral range spans the near-infrared, visible, and near ultraviolet. in all cases except oxidized 2-fe ferredoxins, electronic absorption is observed continuously from less than 5000 cm-1 to above 30,000 cm-1. the ... | 1978 | 728385 |
| chromatium sulfite reductase. i. characterization of thiosulfate-forming activity at the cell extract level. | thiosulfate and sulfide were detected in the sulfite reductase reaction catalyzed by a cell-free extract of photoautotrophically grown chromatium vinosum. hydrogen was consumed upon addition of sulfite to the extract in the presence of hydrogenase and methylviologen. hydrogen uptake proceeded biphasically. during the first phase, thiosulfate and sulfide were formed concomitant with the decrease in sulfite. after the disappearance of sulfite, hydrogen was consumed with reduced velocity and sulfid ... | 1978 | 730752 |
| location of o-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria. | an attempt was made to localize a number of o-methyl sugars in lipopolysaccharides and antigenic polysaccharides isolated from photosynthetic bacteria and from cyano-bacteria. methylation analysis with [2h3]methyl iodide as methylating agent was the method of choice. one has to differentiate between (lipo-)polysaccharides having only trace amounts (less than 1% of polymer dry weight) of o-methyl sugars and those having them in larger amounts (more than 4% of polymer dry weight). in the former ca ... | 1978 | 418776 |
| preparation of chlorophyll a, chlorophyll b and bacteriochlorophyll a by means of column chromatography with diethylaminoethylcellulose. | 1978 | 413574 | |
| rapid sampling culture chamber. | an all-glass chamber for culturing anaerobic and aerobic bacteria in liquid medium is described. the system permits both rapid sampling and turbidimetric measurements under controlled atmospheric conditions. | 1978 | 350159 |
| cluster characterization in iron-sulfur proteins by magnetic circular dichroism. | we report magnetic circular dichroism (mcd) spectra of 4-fe iron-sulfur clusters in the iron-sulfur proteins chromatium high-potential iron protein (hipip), bacillus stearothermophilus ferredoxin and clostridium pasteurianum ferredoxin. the mcd is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. the spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-fe clusters. it is concluded tha ... | 1978 | 281679 |
| purification and properties of adenosine triphosphatase from chromatium vinosum chromatophores. | 1978 | 145959 | |
| [comparative study of b890 pigment-lipoprotein complexes from sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple photosynthesizing bacteria]. | pigment-lipoprotein b890 complexes containing reaction center and "light-focusing" bacteriochlorophyll a were isolated from photosynthetic membranes of sulfur (chromatium minutissimum) and non-sulfur (rhodopseudomonas palustris) purple bacteria after the treatment with triton x-100. the molecular weights of complexes were evaluated using several methods (200 000-300 000). by means of electron microscopy the sizes of complexes were found to be about 80 a. on the air-water interface hexagonal pack ... | 1978 | 207362 |
| structural organization of the chromatium vinosum reaction center associated c-cytochromes. | magnetic interactions operating between the chromatium vinosum reaction center associated c-cytochromes and the electron carriers of the reaction center have been assayed by comparing the magnetic properties of these components alone, and in various combinations with paramagnetic forms of the reaction center electron carriers. these studies have yielded the following results. 1. the oxidized paramagnetic forms of the high potential cytochromes c-555 produce no discernable alteration of the light ... | 1978 | 210808 |
| [epr study of redox titration of chromatium minutissimum chromatophores]. | redox titration of chromatophores through the region +300 +570 mv at room temperature results in generation of two dark esr signals, a well-known reaction center signal and a new narrow line. this line is characterized by deltah pp = 4.2 oe, g-factor about that of rc and a lorentzian shape. it is insensitive to illumination. the determination of an rc signal midpoint potential is affected by the presence of the narrow line. taking into account the superposition of the two esr signals it was meas ... | 1978 | 212119 |
| identification of iron--sulfur clusters in proteins. | 1978 | 213682 | |
| isolation and characterization of a membrane-bound, low-potential c-type cytochrome from purple photosynthetic bacteria, with special reference to rhodospirillum rubrum. | other investigators have isolated soluble, low-potential, c-type cytochromes (cytochrome c3) from a few photosynthetic procaryotes, i.e., a cyanobacterium and two species of purple nonsulfur bacteria. however, such cytochromes appeared to be absent from other purple bacteria, including rhodospirillum rubrum and chromatium vinosum. we now report evidence for the presence of low-potential c-type cytochromes in these two species, in which they were found to be bound to the photosynthetic membranes. ... | 1978 | 214418 |
| electron spin relaxation of iron-sulphur proteins studied by microwave power saturation. | the electron-spin relaxation of iron-sulphur centres in a range of simple proteins (ferredoxin, high-potential iron-sulphur protein and rubredoxin) was investigated by means of the temperature dependence and microwave power saturation of the epr signal. the proteins containing [2fe-2s] centres all showed temperature optima higher than those for [4fe-4s] centres, but the difference between the slowest-relaxing [4fe-4s] protein (chromatium high-potential iron-sulphur protein) and the fastest-relax ... | 1978 | 215217 |
| active transport in the photosynthetic bacterium chromatium vinosum. | 1978 | 30400 | |
| flash-induced changes in the in vivo bacteriochlorophyll fluorescence yield at low temperatures and low redox potentials in carotenoid-containing strains of photosynthetic bacteria. | the changes in the in vivo bacteriochlorophyll fluorescence induced by a xenon flash at low temperatures (77--200 k) with the "primary" acceptor x chemically prereduced have been examined in whole cells of several species of photosynthetic bacteria which contain carotenoids absorbing in the visible part of the absorption spectrum. two groups of species with different behaviour could be distinguished. in both cases a flash-induced rise of the fluorescence yield was observed with x prereduced at 7 ... | 1978 | 96856 |
| nitrogen fixation and hydrogen metabolism in photosynthetic bacteria. | the photosynthetic bacteria are found in a wide range of specialized aquatic environments. these bacteria represent important members of the microbial community since they are capable of carrying out two of the most important processes on earth, namely, photosynthesis and nitrogen fixation, at the expense of solar energy. since the discovery that these bacteria could fix atmospheric nitrogen, there has been an intensification of studies relating to both the biochemistry and physiology of this pr ... | 1978 | 96875 |
| dichroism of bacteriochlorophyll in chromatophores of photosynthetic bacteria. | the dichroism was measured in films of air-dried and, consequently, flattened chromatophores of chromatium vinosum, rhodopseudomonas sphaeroides and rhodospirillum rubrum. the values (deltaa/a) of dichroism in c. vinosum were found to be -1.05 at 590 nm and 0.75 in the near infrared region. the values of dichroism in r. sphaeroides were -0.70 at 590 nm and 0.80 at 870 nm. the values of dichroism in r. rubrum were -1.45 at 590 nm and 0.97 at 870 nm. | 1978 | 97281 |
| ribulose bisphosphate carboxylases from chromatium vinosum and rhodospirillum rubrum and their role in photosynthetic carbon assimilation. | 1978 | 106836 | |
| the preparation and characterization of different types of light-harvesting pigment-protein complexes from some purple bacteria. | a general strategy, with some specific examples, is given for the isolation and purification of detergent-soluble, antenna pigment-protein complexes from the photosynthetic membranes. absorption, fluorescence and circular dichroism spectra, and the pigment and protein composition of b800-b850-protein and b890-protein complexes of some purple bacteria (rhodospirillum rubrum, rhodopseudomonas sphaeroides and rps. capsulata and chromatium vinosum) are discussed. we conclude that there are probably ... | 1978 | 110568 |
| photooxidative damage in photosynthetic activities of chromatium vinosum. | the capacity of photosynthetic co(2) fixation in the anaerobic purple-sulfur bacterium, chromatium vinosum is markedly impaired by strong illumination (9 x 10(4) lux) in the presence of 100% o(2). in the absence of hco(3) (-), decline in activity occurred gradually, with about 40% of the initial activity remaining after a 1-hour incubation. the addition of 50 millimolar hco(3) (-) to the incubation medium resulted in a measurable delay (about 30 minutes) of the inactivation process. ribulose-1,5 ... | 1978 | 16660651 |
| [use of urea by purple bacteria]. | strains of purple sulfur bacteria (chromatium minutissimum, ectothiorhodospira shaposhnikovii, thiocapsa roseopersicina, lamprobacter modestohalophilus) and nonsulfur bacteria (rhodopseudomonas palustris, rh. spheroides, rhodospirillum rubrum) grow in media containing urea as a source of nitrogen at concentrations from 0.5 to 5.0%. they can also utilize the carbon of urea and thus grow in the absence of bicarbonate. urea is decomposed by all the studied purple bacteria with the participation of ... | 1979 | 112359 |
| lipopolysaccharides of photosynthetic prokaryotes. | 1979 | 115382 | |
| oxonol dyes as monitors of membrane potential. their behavior in photosynthetic bacteria. | the reponses of oxonol dyes to single and multiple single turnovers of the photosynthetic apparatus of photosynthetic bacteria have been studied, and compared with the responses of the endogenous carotenoid pigments. the absorbance changes of the oxonols can be conveniently measured at 587 nm, because this is an isosbestic point in the 'light-minus-dark' difference spectrum of the chromatophores. the oxonols appear to respond to the light-induced 'energization' by shifting their absorption maxim ... | 1979 | 103582 |
| regulation of electron transfer in chromatium vinosum chromatophores by intravesicular h+ concentration. | 1979 | 33164 | |
| manganese, an essential trace element for n2 fixation by rhodospirillum rubrum and rhodopseudomonas capsulata: role in nitrogenase regulation. | nitrogenase (n(2)ase) from the photosynthetic bacterium rhodospirillum rubrum can exist in two forms, an unregulated form (n(2)ase a) and a regulatory form (n(2)ase r), the latter being identified in vitro by its need for activation by a mn(2+)-dependent n(2)ase activating system. the physiological significance of this mn(2+)-dependent n(2)ase activating system was suggested here by observations that growth of r. rubrum and rhodopseudomonas capsulata on n(2) gas (a condition that produces active ... | 1979 | 42641 |
| energetic coupling in the primary processes of photosynthesis in chromatium. ph dependence of delayed fluorescence, electron transfer and degree of coupling. | the effects of ph on the thermodynamic properties of the proton-translocating cyclic electron transfer system in a purple photosynthetic bacterium chromatium vinosum were studied. two thermodynamic parameters, the flux (je) and force (deltamue) of the electron transfer process, were analyzed. the rate of electron transfer in the re-reduction of photooxidized reaction-center bacteriochlorophyll was used as je. deltamue was determined from the intensity of the delayed fluorescence from bacteriochl ... | 1979 | 84810 |
| a partial purification of membrane-bound b and c cytochromes from chromatium vinosum. | 1979 | 219774 |