Publications

effects of lsd-25 on avoidance behavior and locomotor activity in mice.	c57bl/6(c57) mice were tested in a shuttle box under the influence of lsd-25 in two sets of experiments. in the first set, the pretrial administrations of lsd (0.05, 0.1, and 0.2 mg/kg) were followed by dose-related performance improvements. in the second set, the posttrial administration of lsd (0.2 mg/kg) immediately after each experimental session improved the performances of the mice. no effect was evident when the hallucinogen was injected 2 h after training, thus showing that lsd improved ...	2000	111278
serum-sickness-like syndrome with membranous glomerulopathy in patient on captopril.		2001	93204
mechanism of the interaction of desipramine with (na+, k+) atpase and p-nitrophenylphosphatase [proceedings].		2001	92937
anatomical characteristics of the oxytalan fibre meshwork.		2001	95162
massive allografts in salvage revisions of failed total knee arthroplasties.	ten patients with failed total knee arthroplasties and severe bone loss were treated with massive whole distal femur and proximal tibial allografts in combination with prosthetic implants. fourteen allografts were inserted either as invaginated or segmental grafts and were rigidly fixed to the host bone. clinically and roentgenographically, 12 of 14 grafts (86%) seemed to have united to the host bone. the average range of motion was 92 degrees. five patients developed complications; two of these ...	2001	2225617
primary in vitro cytotoxic response of nzb spleen cells to qa-1b-associated antigenic determinants.	we have shown that cytotoxic lymphocytes generated in primary cultures of nzb spleen cells with h-2-identical balb/c or b10.d2 stimulator cells exhibit specificity for qa-1b-associated antigenic determinants. this unidirectional cytotoxicity constitutes the initial demonstration of a primary in vitro response to antigens of the qa-tla system. such responses do not require h-2 homology between effector and target cells in the assay system. in fact, when h-2dd homologous target cells were employed ...	2001	92525
serum protein polymorphisms in a village community from the gambia, west africa (hp, tf, and gc).	serum samples from 857 inhabitants of the village of keneba, the gambia, west africa, were examined by means of polyacrylamide gel electrophoresis. in 203 cases no haptoglobin could be detected, whilst in the remaining 654 samples the three common haptoglobin phenotypes were found with gene frequencies of 0.651 (hp1) and 0.349 (hp2). the d1 transferrin variant gene was found with a frequency of 0.025. in the serum gc system the fast variant gc-ab was detected, the gene frequencies being: gc1, 0. ...	2001	88407
interferon therapy in myelomatosis.		2001	87719
a third crystal form of wolinella succinogenes quinol:fumarate reductase reveals domain closure at the site of fumarate reduction.	quinol:fumarate reductase (qfr) is a membrane protein complex that couples the reduction of fumarate to succinate to the oxidation of quinol to quinone. previously, the crystal structure of qfr from wolinella succinogenes was determined based on two different crystal forms, and the site of fumarate binding in the flavoprotein subunit a of the enzyme was located between the fad-binding domain and the capping domain [lancaster, c.r.d., kröger, a., auer, m., & michel, h. (1999) nature 402, 377--385 ...	2001	11248702
probing the active site of l-aspartate oxidase by site-directed mutagenesis: role of basic residues in fumarate reduction.	l-aspartate oxidase is a very particular oxidase which behaves as a fumarate reductase in anaerobic conditions. its primary and tertiary structures present remarkable similarity with the soluble fumarate reductase (frd) from shewanella frigidimarina and the flavin subunit of the membrane-bound fumarate reductase from escherichia coli and wolinella succinogenes. this and other extensive similarities are consistent with the idea that a common catalytic mechanism for the reduction of fumarate opera ...	2001	11294641
the carboxin-binding site on paracoccus denitrificans succinate:quinone reductase identified by mutations.	succinate:quinone reductase catalyzes electron transfer from succinate to quinone in aerobic respiration. carboxin is a specific inhibitor of this enzyme from several different organisms. we have isolated mutant strains of the bacterium paracoccus denitrificans that are resistant to carboxin due to mutations in the succinate:quinone reductase. the mutations identify two amino acid residues, his228 in sdhb and asp89 in sdhd, that most likely constitute part of a carboxin-binding site. this site i ...	2001	11456223
succinate:quinone oxidoreductases--what can we learn from wolinella succinogenes quinol:fumarate reductase?	the structure of wolinella succinogenes quinol:fumarate reductase by x-ray crystallography has been determined at 2.2-a resolution [lancaster et al. (1999), nature 402, 377-385]. based on the structure of the three protein subunits a, b, and c and the arrangement of the six prosthetic groups (a covalently bound fad, three iron-sulphur clusters, and two haem b groups) a pathway of electron transfer from the quinol-oxidising dihaem cytochrome b in the membrane to the site of fumarate reduction in ...	2001	11532445
periplasmic methacrylate reductase activity in wolinella succinogenes.	the cell homogenate and the soluble cell fraction of wolinella succinogenes grown with formate and fumarate catalyzed the oxidation of benzyl viologen radical by methacrylate [apparent km=0.23 mm, vmax=1.0 u (mg cell protein) -1] or acrylate [apparent km=0.50 mm, vmax=0.77 u (mg cell protein) -1]. crotonate did not serve as an oxidant. a mutant of w. succinogenes lacking the fccabc operon was unable to catalyze methacrylate or acrylate reduction. in contrast, the inactivation of fccc alone had n ...	2001	11685377
the tetraheme cytochrome c nrfh is required to anchor the cytochrome c nitrite reductase (nrfa) in the membrane of wolinella succinogenes.	the electron-transport chain that catalyzes nitrite respiration with formate in wolinella succinogenes consists of formate dehydrogenase, menaquinone and the nitrite reductase complex. the latter catalyzes nitrite reduction by menaquinol and is made up of nrfa and nrfh, two c-type cytochromes. nrfa is the catalytic subunit; its crystal structure is known. nrfh belongs to the napc/nirt family of membrane-bound c-type cytochromes and mediates electron transport between menaquinol and nrfa. it is d ...	2001	11722563
a suggested approach to malaria control and to the methodology applicable in different epidemiologic situations, based on experience in the americas.		2001	93006
fumarate respiration of wolinella succinogenes: enzymology, energetics and coupling mechanism.	wolinella succinogenes performs oxidative phosphorylation with fumarate instead of o2 as terminal electron acceptor and h2 or formate as electron donors. fumarate reduction by these donors ('fumarate respiration') is catalyzed by an electron transport chain in the bacterial membrane, and is coupled to the generation of an electrochemical proton potential (deltap) across the bacterial membrane. the experimental evidence concerning the electron transport and its coupling to deltap generation is re ...	2002	11803015
succinate:quinone oxidoreductase in the bacteria paracoccus denitrificans and bacillus subtilis.	an overview of the present knowledge about succinate:quinone oxidoreductase in paracoccus denitrificans and bacillus subtilis is presented. p. denitrificans contains a monoheme succinate:ubiquinone oxidoreductase that is similar to that of mammalian mitochondria with respect to composition and sensitivity to carboxin. results obtained with carboxin-resistant p. denitrificans mutants provide information about quinone-binding sites on the enzyme and the molecular basis for the resistance. b. subti ...	2002	11803018
succinate:quinone oxidoreductases from epsilon-proteobacteria.	the epsilon-proteobacteria form a subdivision of the proteobacteria including the genera wolinella, campylobacter, helicobacter, sulfurospirillum, arcobacter and dehalospirillum. the majority of these bacteria are oxidase-positive microaerophiles indicating an electron transport chain with molecular oxygen as terminal electron acceptor. however, numerous members of the epsilon-proteobacteria also grow in the absence of oxygen. the common presence of menaquinone and fumarate reduction activity su ...	2002	11803019
crystallization and preliminary x-ray analysis of the membrane-bound cytochrome c nitrite reductase complex (nrfha) from wolinella succinogenes.	crystals of the complex between the enzyme cytochrome c nitrite reductase (nrfa) and the membrane-bound quinol oxidase and electron carrier nrfh were grown by vapour diffusion using ammonium sulfate as a precipitant. in the epsilon-proteobacterium wolinella succinogenes, nrfa and nrfh form a functional membrane-bound complex which catalyzes the last step in the metabolic pathway of nitrate dissimilation. nrfh represents a prototype of a large family of putative bacterial quinol oxidases, the nap ...	2002	11807271
the function of methyl-menaquinone-6 and polysulfide reductase membrane anchor (psrc) in polysulfide respiration of wolinella succinogenes.	wolinella succinogenes grows by oxidative phosphorylation with polysulfide as terminal electron acceptor and either h2 or formate as electron donor (polysulfide respiration). the function of the respiratory chains catalyzing these reactions was investigated. proteoliposomes containing polysulfide reductase (psr) and either hydrogenase or formate dehydrogenase isolated from the membrane fraction of wolinella succinogenes catalyzed polysulfide respiration, provided that methyl-menaquinone-6 isolat ...	2002	11856339
structure and spectroscopy of the periplasmic cytochrome c nitrite reductase from escherichia coli.	the crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (nrfa) of escherichia coli are presented. the structure is the first for a member of the nrfa subgroup that utilize a soluble penta-heme cytochrome, nrfb, as a redox partner. comparison to the structures of wolinella succinogenes nrfa and sulfospirillum deleyianum nrfa, which accept electrons from a membrane-anchored tetra-heme cytochrome (nrfh), reveals notable differences in the prot ...	2002	11863430
the nrfi gene is essential for the attachment of the active site haem group of wolinella succinogenes cytochrome c nitrite reductase.	the cytochrome c nitrite reductase complex (nrfha) is the terminal enzyme in the electron transport chain catalysing nitrite respiration of wolinella succinogenes. the catalytic subunit nrfa is a pentahaem cytochrome c containing an active site haem group which is covalently bound via the cysteine residues of a unique cwtck motif. the lysine residue serves as the axial ligand of the haem iron. the other four haem groups of nrfa are bound by conventional haem-binding motifs (cxxch). the nrfhaij l ...	2002	11929530
reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from wolinella succinogenes.	hydrogenase and fumarate reductase isolated from wolinella succinogenes were incorporated into liposomes containing menaquinone. the two enzymes were found to be oriented solely to the outside of the resulting proteoliposomes. the proteoliposomes catalyzed fumarate reduction by h2 which generated an electrical proton potential (delta(psi) = 0.19 v, negative inside) in the same direction as that generated by fumarate respiration in cells of w. succinogenes. the h+/e ratio brought about by fumarat ...	2002	11952800
cysteine-mediated electron transfer in syntrophic acetate oxidation by cocultures of geobacter sulfurreducens and wolinella succinogenes.	syntrophic cocultures of geobacter sulfurreducens and wolinella succinogenes oxidize acetate with nitrate as terminal electron acceptor. it has been postulated earlier that electrons are transferred in these cocultures not via hydrogen, but via a different carrier, e.g., a small c-type cytochrome that is detected in the supernatant of growing cultures. in the present study, l -cysteine, which was provided as a reducing agent, was found to mediate the electron transfer between the two partners. l ...	2002	12070769
modification of heme c binding motifs in the small subunit (nrfh) of the wolinella succinogenes cytochrome c nitrite reductase complex.	the two multiheme c-type cytochromes nrfh and nrfa form a membrane-bound complex that catalyzes menaquinol oxidation by nitrite during respiratory nitrite ammonification of wolinella succinogenes. each cysteine residue of the four nrfh heme c binding motifs was individually replaced by serine. of the resulting eight w. succinogenes mutants, only one is able to grow by nitrite respiration although its electron transport activity from formate to nitrite is decreased. nrfh from this mutant was show ...	2002	12095623
enzymology and bioenergetics of respiratory nitrite ammonification.	nitrite is widely used by bacteria as an electron acceptor under anaerobic conditions. in respiratory nitrite ammonification an electrochemical proton potential across the membrane is generated by electron transport from a non-fermentable substrate like formate or h(2) to nitrite. the corresponding electron transport chain minimally comprises formate dehydrogenase or hydrogenase, a respiratory quinone and cytochrome c nitrite reductase. the catalytic subunit of the latter enzyme (nrfa) catalyzes ...	2002	12165429
psrr, a member of the arac family of transcriptional regulators, is required for the synthesis of wolinella succinogenes polysulfide reductase.	wolinella succinogenes grows by polysulfide respiration with formate or hydrogen as electron donor. polysulfide reduction is catalyzed by the membrane-bound polysulfide reductase complex encoded by the psrabc operon. an open reading frame, designated psrr, was found in close proximity upstream of the psr operon, but oriented in the opposite direction. the deduced amino acid sequence of psrr is similar to those of transcriptional regulators of the arac family and includes all typical features. po ...	2002	12189421
wolinella succinogenes quinol:fumarate reductase-2.2-a resolution crystal structure and the e-pathway hypothesis of coupled transmembrane proton and electron transfer.	the structure of the respiratory membrane protein complex quinol:fumarate reductase (qfr) from wolinella succinogenes has been determined by x-ray crystallography at 2.2-a resolution [nature 402 (1999) 377]. based on the structure of the three protein subunits a, b, and c and the arrangement of the six prosthetic groups (a covalently bound fad, three iron-sulfur clusters, and two haem b groups), a pathway of electron transfer from the quinol-oxidising dihaem cytochrome b in the membrane to the s ...	2002	12409197
specificity of respiratory pathways involved in the reduction of sulfur compounds by salmonella enterica.	the tetrathionate (ttr) and thiosulfate (phs) reductases of salmonella enterica lt2, together with the polysulfide reductase (psr) of wolinella succinogenes, are unusual examples of enzymes containing a molybdopterin active-site cofactor since all formally catalyse sulfur-sulfur bond cleavage. this is in contrast to the oxygen or hydrogen transfer reactions exhibited by other molybdopterin enzymes. here the catalytic specificity of ttr and phs has been compared using both physiological and synth ...	2002	12427953
a group-specific pcr assay for the detection of helicobacteraceae in human gut.	enterohepatic helicobacter species are emerging pathogens, which are increasingly isolated from humans with enteric diseases. nevertheless, current methods to detect helicobacteraceae in the human gut have significant limitations.	2002	12485125
[on the problem complex of the new act on the severely handicapped with respect to the workshop for the disabled (author's transl)].	one group of the disabled, i.e., the most severely handicapped, will not be able to benefit from the improvements brought about by the new act on the severely handicapped, if the statutory orders of the federal government and the provisions of the federal employment office are implemented as planned. in this event it would become extremely difficult for the severely physically handicapped to participate in the life of the community. the article demonstrates how a sheltered workshop for the disab ...	2002	135322
pancreatitis and choledochal cyst with gallstones: an unusual case.		2002	123899
surgical treatment of bronchiectasis in kartagener's syndrome.	a case of kartagener's syndrome in which bilateral partial pulmonary resection was performed is reported. the literature concerning the surgical treatment of severe bronchiectasis is reviewed.	2002	1084204
location and sequence of atherosclerotic plaque formation in white carneau and show racer pigeons: reevaluation and redefinition.	sudan staining of gross specimens with correlated histologic sections was used to localize the accumulation of fat in the aortas of genetically susceptible and resistant strains of pigeons. the birds were maintained on a cholesterol-free grain diet, and studies were done sequentially from ages 1 month to 4 years. evidence suggests that the accumulation of lipids in musculoelastic cushions located at the origin of small branches is not necessarily a precursor of complicated lesions, but that it o ...	2003	152618
metabolic effects of fructose in the liver.		2003	208819
the structure of wolinella succinogenes quinol: fumarate reductase and its relevance to the superfamily of succinate: quinone oxidoreductases.		2003	12629969
multifrequency cw-epr investigation of the catalytic molybdenum cofactor of polysulfide reductase from wolinella succinogenes.	electron paramagnetic resonance (epr) spectra of the molybdenum centre in polysulfide reductase (psr) from wolinella succinogenes with unusually high g-tensor values have been observed for the first time. three different mo(v) states have been generated (by the addition of the substrate polysulfide and different redox agents) and analysed by their g- and hyperfine tensors using multifrequency (s-, x- and q-band) cw-epr spectroscopy. the unusually high g-tensor values are attributed to a large nu ...	2003	12761663
electron transport to periplasmic nitrate reductase (napa) of wolinella succinogenes is independent of a napc protein.	the rumen bacterium wolinella succinogenes grows by respiratory nitrate ammonification with formate as electron donor. whereas the enzymology and coupling mechanism of nitrite respiration is well known, nitrate reduction to nitrite has not yet been examined. we report here that intact cells and cell fractions catalyse nitrate and chlorate reduction by reduced viologen dyes with high specific activities. a gene cluster encoding components of a putative periplasmic nitrate reductase system (napa, ...	2003	12823811
genotypic identification of erythromycin-resistant campylobacter isolates as helicobacter species and analysis of resistance mechanism.	the correct identification of campylobacter species remains cumbersome, especially when conventional biochemical tests and antimicrobial susceptibility patterns are used for a phenotypical identification. correct identification is important for epidemiological purposes and for studying changes in antimicrobial resistance patterns. six erythromycin-resistant campylobacter strains were investigated by 16s ribosomal dna (rdna) sequencing, 23s rdna sequencing, and restriction fragment length polymor ...	2003	12904383
complete genome sequence and analysis of wolinella succinogenes.	to understand the origin and emergence of pathogenic bacteria, knowledge of the genetic inventory from their nonpathogenic relatives is a prerequisite. therefore, the 2.11-megabase genome sequence of wolinella succinogenes, which is closely related to the pathogenic bacteria helicobacter pylori and campylobacter jejuni, was determined. despite being considered nonpathogenic to its bovine host, w. succinogenes holds an extensive repertoire of genes homologous to known bacterial virulence factors. ...	2003	14500908
the hyde gene is essential for the formation of wolinella succinogenes nife-hydrogenase.	wolinella succinogenes grows by anaerobic respiration using hydrogen gas as electron donor. the hyde gene is located on the genome downstream of the structural genes encoding the membrane-bound nife-hydrogenase complex (hydabc) and a putative protease (hydd) possibly involved in hydrogenase maturation. homologs of hyde are found in the vicinity of nife-hydrogenase-encoding genes on the genomes of several other proteobacteria. a hyde deletion mutant of w. succinogenes does not catalyze hydrogen o ...	2003	14592709
purification and properties of the formate dehydrogenase and characterization of the fdha gene of sulfurospirillum multivorans.	the soluble periplasmic subunit of the formate dehydrogenase fdha of the tetrachloroethene-reducing anaerobe sulfurospirillum multivorans was purified to apparent homogeneity and the gene ( fdha) was identified and sequenced. the purified enzyme catalyzed the oxidation of formate with oxidized methyl viologen as electron acceptor at a specific activity of 1683 nkat/mg protein. the apparent molecular mass of the native enzyme was determined by gel filtration to be about 100 kda, which was confirm ...	2003	14610638
wolinella succinogenes quinol:fumarate reductase and its comparison to e. coli succinate:quinone reductase.	the three-dimensional structure of wolinella succinogenes quinol:fumarate reductase (qfr), a dihaem-containing member of the superfamily of succinate:quinone oxidoreductases (sqor), has been determined at 2.2 a resolution by x-ray crystallography [lancaster et al., nature 402 (1999) 377-385]. the structure and mechanism of w. succinogenes qfr and their relevance to the sqor superfamily have recently been reviewed [lancaster, adv. protein chem. 63 (2003) 131-149]. here, a comparison is presented ...	2003	14630313
the quaternary structure of the saccharomyces cerevisiae succinate dehydrogenase. homology modeling, cofactor docking, and molecular dynamics simulation studies.	succinate dehydrogenases and fumarate reductases are complex mitochondrial or bacterial respiratory chain proteins with remarkably similar structures and functions. succinate dehydrogenase oxidizes succinate and reduces ubiquinone using a flavin adenine dinucleotide cofactor and iron-sulfur clusters to transport electrons. a model of the quaternary structure of the tetrameric saccharomyces cerevisiae succinate dehydrogenase was constructed based on the crystal structures of the escherichia coli ...	2004	14672929
solution structure of the 30 kda polysulfide-sulfur transferase homodimer from wolinella succinogenes.	the periplasmic polysulfide-sulfur transferase (sud) protein encoded by wolinella succinogenes is involved in oxidative phosphorylation with polysulfide-sulfur as a terminal electron acceptor. the polysulfide-sulfur is covalently bound to the catalytic cys residue of the sud protein and transferred to the active site of the membranous polysulfide reductase. the solution structure of the homodimeric sud protein has been determined using heteronuclear multidimensional nmr techniques. the structure ...	2004	14769017
the unprecedented nos gene cluster of wolinella succinogenes encodes a novel respiratory electron transfer pathway to cytochrome c nitrous oxide reductase.	the -proteobacterium wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. nevertheless, it is capable of n(2)o reduction to n(2). recently, the genome sequence of w. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of n(2)o reductase. the predicted enzyme is similar to other n(2)o reductases exhibiting conservation of all residues ligating the two multinuclear copper centers but carries an unprecedented ...	2004	15225600
structural and functional analogue of the active site of polysulfide reductase from wolinella succinogenes.	synthesis of [pph4]2[mo(sph)2(s2c2(cn)2)2] (2) from [pph4]2[moo(s2c2(cn)2)2] (1) has been achieved to mimic the postulated [mo(s)6] core of polysulfide reductase with two thiolates and two bis(ene-dithiolate) ligands. compound 2 reacts with polysulfide to yield h2s, modeling the function of polysulfide reductase. the facile conversion of 2 back to 1 in moist solvent suggests that the interconversion of the [moiv = o] and [moiv - x] (x = o-ser, s-cys, se-cys) moieties might occur in the dmso redu ...	2004	15257571
species of the family helicobacteraceae detected in an australian sea lion (neophoca cinerea) with chronic gastritis.	we describe the first case of gastritis in a male australian sea lion (neophoca cinerea) in which members of the family helicobacteraceae, particularly the genus wolinella, were detected. the sea lion exhibited clinical signs of gastrointestinal disease, including abdominal pain, lack of appetite, and lethargy. examination of one ileal and five gastric biopsy specimens collected over a 10-year period revealed persistent fibrosis and/or superficial focal erosion and ulceration of the lamina propr ...	2004	15297490
the fdh operon of sulfurospirillum multivorans.	the complete single copy fdh operon (approximately 5.7 kb) encoding the formate dehydrogenase subunits of the gram negative, reductively dehalogenating anaerobe sulfurospirillum multivorans was sequenced and analyzed. the gene fdha encoding the catalytically active periplasmic subunit is part of an operon (fdheabcd) containing additional structural genes. the genes fdheabcd were cotranscribed as indicated by rt-pcr and primer extension experiments. two mrnas for fdheabcd and fdhabcd were either ...	2004	15321667
calculated coupling of transmembrane electron and proton transfer in dihemic quinol:fumarate reductase.	the quinol:fumarate reductase of wolinella succinogenes binds a low- and a high-potential heme b group in its transmembrane subunit c. both hemes are part of the electron transport chain between the two catalytic sites of this redox enzyme. the oxidation-reduction midpoint potentials of the hemes are well established but their assignment in the structure has not yet been determined. by simulating redox titrations, using continuum electrostatics calculations, it was possible to achieve an unequiv ...	2004	15361415
n-linked protein glycosylation is required for full competence in campylobacter jejuni 81-176.	the recent sequencing of the virulence plasmid of campylobacter jejuni 81-176 revealed the presence of genes homologous to type iv secretion systems (tfss) that have subsequently been found in helicobacter pylori and wolinella succinogenes. mutational analyses of some of these genes have implicated their involvement in intestinal epithelial cell invasion and natural competence. in this report, we demonstrate that one of these type iv secretion homologs, cjp3/virb10, is a glycoprotein. treatment ...	2004	15375132
comparative analysis of four campylobacterales.	comparative genome analysis can be used to identify species-specific genes and gene clusters, and analysis of these genes can give an insight into the mechanisms involved in a specific bacteria-host interaction. comparative analysis can also provide important information on the genome dynamics and degree of recombination in a particular species. this article describes the comparative genome analysis of representatives of four different campylobacterales species - two pathogens of humans, helicob ...	2004	15494744
characterization of the menaquinone reduction site in the diheme cytochrome b membrane anchor of wolinella succinogenes nife-hydrogenase.	the majority of bacterial membrane-bound nife-hydrogenases and formate dehydrogenases have homologous membrane-integral cytochrome b subunits. the prototypic nife-hydrogenase of wolinella succinogenes (hydabc complex) catalyzes h2 oxidation by menaquinone during anaerobic respiration and contains a membrane-integral cytochrome b subunit (hydc) that carries the menaquinone reduction site. using the crystal structure of the homologous fdni subunit of escherichia coli formate dehydrogenase-n as a m ...	2004	14576151
myeloid and erythroid progenitor cells from normal bone marrow adhere to collagen type i.	one of the mechanisms by which normal hematopoietic progenitor cells remain localized within the bone marrow microenvironment is likely to involve adhesion of these cells to extracellular matrix (ecm) proteins. for example, there is evidence that uncommitted, hla-dr-negative progenitor cells and committed erythroid precursors (bfu-e) bind to fibronectin. however, fibronectin is not known to mediate binding of committed myeloid (granulocyte-macrophage) progenitors, raising the possibility that ot ...	2004	1370640
detection and widespread distribution of the nrfa gene encoding nitrite reduction to ammonia, a short circuit in the biological nitrogen cycle that competes with denitrification.	degenerate primers to detect nrfa were designed by aligning six nrfa sequences including escherichia coli k-12, sulfurospirillum deleyianum and wolinella succinogenes. these primers amplified a 490 bp fragment of nrfa. the ability of these primers to detect nrfa was tested with chromosomal dna isolated from a variety of bacteria: they could distinguish between bacteria in which the gene is known to be present or absent. the positive reference organisms spanned the various classes of proteobacter ...	2004	19712292
[therapeutic endoscopy in the gastrointestinal tract].	in selected chapters of the therapeutic endoscopy we tried to represent secure knowledge, actual tendencies and partly future prognoses. hereby completeness cannot be expected. however, it became clear that numerous physical principles became useful for the therapy with the endoscope. chemical techniques, such as the litholysis of gall-stones are only at present used in our field. with all scepticism against the own field of interest one may say that the operative therapeutic endoscopy, as it is ...	2004	539002
inducibility of metallothionein biosynthesis in cultured normal and menkes kinky hair disease fibroblasts: effects of copper and cadmium.	metallothionein biosynthesis is not induced by extracellular copper in menkes kinky hair disease (mkhd) or in normal cultured fibroblasts under the conditions of these experiments. in the presence of copper, mkhd fibroblasts also incorporated less cysteine than did normal fibroblasts. extracellular cadmium greatly enhanced the uptake of cysteine in both normal and mkhd cultures. by the technique of polyacrylamide gel electrophoresis, it was demonstrated that metallothionein is induced by cadmium ...	2004	471576
[low-molecular weight nuclear rna's].		2004	395786
professor robert-henri regamey, 1907-1978.		2004	387795
[eeg-activity patterns in visual information processing: characteristics, variance-analytical deliminiation, discriminance-analytical determination of undispensable variables and scale level].	1. in order to determine the variable distributions of 5 activation dependent eeg activity patterns occurring during visual information processing, mean values and standard deviations of the percental quantities of the frequencies 4, 5, ..., 13 hz, 14 to 20 hz and 21 to 30 hz, as well as the mean amplitudes in the frequency bands 3.5 ... 7.4 hz, 7.5 ... 13.4 hz and 13.5 to 30 hz were determined on corresponding to 10 s samples. it could be demonstrated by regression analysis that an interval sca ...	2004	525137
looking back. nursing in diseases of the kidneys; by dr. f. w. hall. august 1908.		2004	390275
[use of mathematics in pathological anatomy].	the author discusses the possibilities of using mathematics in pathological anatomy studies on the basis of data from literature and his own studies. the information on the organization and planning of a mathematical investigation and on the features of the system approach to investigation of pathomorphology problems is presented. the main stages of the mathematical analysis of pathological changes are described with special reference to the use of likelihood and information approaches to the ev ...	2004	356813
the function of the follicular epithelium in vitellogenic oncopeltus follicles.	autoradiographic and electrophoretic methods that have detected an endogenously synthesized protein yolk component in hyalophora cecropia follicles failed to reveal such a protein in oncopeltus fasciatus. similarly, neither sulfate nor glucosamine was incorporated into the intercellular matrix of vitellogenic follicles, though both label these regions intensely in hyalophora. while both insects produce yolk from hemolymph vitellogenin, their follicles thus appear to support the process by very d ...	2004	524343
[combined chylothorax and chylous ascites in cirrhosis].		2004	520918
projection arteriography and venography: initial clinical results with mr.	motion currently limits the applications of magnetic resonance (mr) angiography in certain regions of the body. to overcome this problem, a series of breath-hold, two-dimensional, flow-compensated gradient-echo images were acquired. these images were then processed by means of the maximum intensity projection algorithm to produce projection angiograms. the method was evaluated in 10 healthy subjects and in 12 patients and validated by comparing conventional angiograms, contrast material-enhanced ...	2004	2748814
projection arteriography and venography: initial clinical results with mr.	motion currently limits the applications of magnetic resonance (mr) angiography in certain regions of the body. to overcome this problem, a series of breath-hold, two-dimensional, flow-compensated gradient-echo images were acquired. these images were then processed by means of the maximum intensity projection algorithm to produce projection angiograms. the method was evaluated in 10 healthy subjects and in 12 patients and validated by comparing conventional angiograms, contrast material-enhanced ...	2004	2748814
job sharing: a viable option.		2004	1304937
cellular origin of transforming genes of rna tumor viruses.		2004	400607
physiological response patterns during "intake" and "rejection" tasks.	two 2-min tasks, one requiring outer-directed attention (intake task), the other a mental arithmetic task (rejection task) were presented to 20 subjects. according to lacey the distinguishing feature of the response to intake tasks are cardiac deceleration and blood pressure decreases. none of these responses was obtained. some variables, however, were only affected by one of the tasks; the intake task led to a decreased hand (skin) blood flow and an increased hand vascular resistance, whereas a ...	2004	534671
verbal effects in children's visual recognition memory.	preschool children were shown line drawings of 12 pairs of items and were asked to describe them. each child saw elaborated and unelaborated pictures (items interacting vs. not interacting). the children's descriptions were rated as elaborated or unelaborated (interactions mentioned vs. not mentioned). 1 week later, a recognition test was given, with choices between an elaborated and an unelaborated picture for each pair. in general, recognition accuracy was best for elaborated pictures given el ...	2005	1183272
improved methodology and precision using a straight base line technique for the quantitation of proteins by two dimensional immunoelectrophoresis.		2005	1112067
[cysts and fistulas of embryonal origin of the face].		2005	1064061
[approaches to the care of pelvic fractures].		2005	546828
hydrocelectomy. evaluation of technique.	a quick, simple, safe, and efficient means of hydrocelectomy is presented. the technique consists of extruding and fixing the testicle into a scrotal pouch. twenty hydroceles were repaired without complication or recurrence.	2005	1265943
linkage studies between hl-a and gpt polymorphisms and other genetic markers.	the phenotypes of 56 families with 126 children from the hamburg area as well as gene frequencies and segregation of the genetic markers gpt, ap, ada, ak, pgm1, pgm3, 6-pgd, che, c3, gc, tf, hp and cp were studied. in regard to linkage, the informative families were correlated to the results of hl-a and gpt typing. the linkage was tested according to the sequential test by morton (1955). see article. for other gene loci, linkage to the hl-a or gpt system could not be proved. but the positive lod ...	2005	1251571
the impact of smoking on mechanical properties of the lungs in idiopathic pulmonary fibrosis and sarcoidosis.	interstitial lung disease is characterized by interstitial inflammatory cell infiltration and fibrosis, a reduction in lung volumes, an increase in lung elastic recoil, and rapid shallow respirations. however, the alterations in lung volumes and elastic recoil as well as in breathing pattern are extremely variable, and the values are normal in a number of patients. in order to determine whether the effects of smoking could account for the variability in lung volumes and breathing pattern, we eva ...	2005	1952439
improving physical therapy students' clinical problem-solving skills: an analytical questioning model.	the purpose of this study was to assess the effectiveness of a model for teaching problem-solving skills to first-year physical therapy students using the watson-glaser critical thinking appraisal (cta) and participant feedback. we used a pretest-posttest control group design. subjects were 31 first-year physical therapy students divided into a control group (n = 15) and an experimental group (n = 16). students in the experimental group used the problem-solving model during a four-week clerkship ...	2005	2727067
[chemotherapy in hemodialyzed patients].		2005	1808351
gonadotropin-stimulated uptake of steroid hormones by rana pipiens ovarian follicles incubated in vitro.		2005	1082831
palpatory estimation of liver size. within- and between-observer variation.	a study based upon 23 patients revealed substantial within- and between-observer variation, when 14 senior and junior surgeons under blindfold conditions were requested to measure the distance from the thoracic cage to the lower border of the liver in the midclavicular and midsternal lines. clinical decisions should accordingly not rely heavily upon a palpatory estimate of liver size.	2005	539331
micronodular encephalitis and acute leukemia treated with immunosuppressives in a child.	the present paper is the study of a micronodular encephalitis with inclusions occurring in a child suffering from acute leukemia treated with antimitotics and immunosuppressives. the role of the immunosuppressive treatment in the onset of the encephalitic syndrome is discussed.	2005	1242214
[therapeutic effect of complamine in atherosclerosis and ischemic heart disease].		2005	1273633
phloroglucinol derivatives of eleven dryopteris species from japan.		2005	1197418
[paramedical personnel in psychiatry: specifics of training in a military teaching hospital].		2005	1343486
the apical a wave versus the fourth heart sound in assessing the severity of aortic stenosis.	the height of the "a" wave of the apexcardiogram was evaluated as a marker for critical aortic stenosis in patients over 40. critical aoritc stenosis was defined as an aortic valve area less than .75 cm-2 with no more than mild aortic insufficiency. phonocardiograms and apexcardiograms were performed on 72 patients with catheterization proven aortic stenosis and on 14 normal controls, all over age 40. the height of the "a" wave of the apexcardiogram was measured as a percentage of the e to o exc ...	2005	1112012
the small for dates infant.		2005	1245399
part ii: the photic environment of clear tropical seas during the day.		2005	1166605
[practical evaluation of methods in pharmacokinetics].	a short discussion of the differences between kinetic processes of zero and first order is followed by an outline of evaluation methods used in pharmacokinetic practice, e.g. "paper and pencil methods" as well as the use of digital and analog computers. the increasing potency of new drugs requires not only more sophisticated methods of analysis and expensive facilities but also more complex models to generate pharmacokinetic information.	2005	1242344
pattern and depth discrimination from random dot stereograms.	the discrimination of depth and pattern was investigated using random dot stereograms. for all four subjects, the psychometric function for depth judgments (probability of correct response) was clearly superior to pattern judgments over a range of ten crossed or uncrossed binocular disparity values. degrading the visual images, either monocularly or binocularly, with various convex lens power (fogging lenses) reduced both depth and pattern discrimination across all disparity values, rather than ...	2005	1130488
failure to consult specialist.		2005	1173090
[radiotherapy in the cancer of the rectum (author's transl)].		2005	1199662
letter: regulation of medical practice by government.		2005	1178002
xeromammography at a community hospital. a year's experience.		2005	1183210
experimental support for the "e pathway hypothesis" of coupled transmembrane e- and h+ transfer in dihemic quinol:fumarate reductase.	reconciliation of apparently contradictory experimental results obtained on the quinol:fumarate reductase, a diheme-containing respiratory membrane protein complex from wolinella succinogenes, was previously obtained by the proposal of the so-called "e pathway hypothesis." according to this hypothesis, transmembrane electron transfer via the heme groups is strictly coupled to cotransfer of protons via a transiently established pathway thought to contain the side chain of residue glu-c180 as the ...	2005	16380425
[unsaturated organic acids as terminal electron acceptors for reductase chains of anaerobic bacteria].	this paper summarizes the current knowledge of unsaturated organic acids in their role as terminal electron acceptors of anaerobic bacteria. the mechanisms and enzyme systems involved in the reduction of fumarate by escherichia coli, wolinella succinogenes, and some species of the genus shewanella are considered. particular attention is given to reduction of the double bond of the unnatural compound methacrylate by the sigma-proteobacterium geobacter sulfurreducens am-1. soluble periplasmic flav ...	2005	16400981
site-directed modifications indicate differences in axial haem c iron ligation between the related nrfh and napc families of multihaem c-type cytochromes.	during the last decade, a number of related bacterial membrane-bound multihaem c-type cytochromes, collectively referred to as the napc/nirt family, were identified. these proteins are generally thought to catalyse electron transport between the quinone/quinol pool and periplasmic oxidoreductases. the best-characterized members, the tetrahaem c-type cytochromes nrfh and napc, mediate electron transport to nrfa and napa respectively. amino acid sequence alignments suggest that the nature and posi ...	2005	15907193
a census of membrane-bound and intracellular signal transduction proteins in bacteria: bacterial iq, extroverts and introverts.	analysis of complete microbial genomes showed that intracellular parasites and other microorganisms that inhabit stable ecological niches encode relatively primitive signaling systems, whereas environmental microorganisms typically have sophisticated systems of environmental sensing and signal transduction.	2005	15955239
discordant 16s and 23s rrna gene phylogenies for the genus helicobacter: implications for phylogenetic inference and systematics.	analysis of 16s rrna gene sequences has become the primary method for determining prokaryotic phylogeny. phylogeny is currently the basis for prokaryotic systematics. therefore, the validity of 16s rrna gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. discrepancies between 16s rrna gene analyses and dna-dna hybridization and phenotypic analyses have been noted in the genus helicobacter. to clarify these discrepancies, we sequenced the 23s rrna genes for ...	2005	16109952
ftir difference spectra of wolinella succinogenes quinol:fumarate reductase support a key role of glu c180 within the "e-pathway hypothesis" of coupled transmembrane electron and proton transfer.	electrochemically induced static ftir difference spectroscopy has been employed to investigate redox-driven protonation changes of individual amino acid residues in the quinol:fumarate reductase (qfr) from wolinella succinogenes. the difference spectra presented were taken in the mid-infrared region from 1800 to 1000 cm(-1), and the signals obtained represent transitions between the reduced and oxidized states of the enzyme. analysis of the difference spectra shows evidence for structural reorga ...	2005	16229484
probing heme propionate involvement in transmembrane proton transfer coupled to electron transfer in dihemic quinol:fumarate reductase by 13c-labeling and ftir difference spectroscopy.	quinol:fumarate reductase (qfr) is the terminal enzyme of anaerobic fumarate respiration. this membrane protein complex couples the oxidation of menaquinol to menaquinone to the reduction of fumarate to succinate. although the diheme-containing qfr from wolinella succinogenes is known to catalyze an electroneutral process, its three-dimensional structure at 2.2 a resolution and the structural and functional characterization of variant enzymes revealed locations of the active sites that indicated ...	2005	16342962