Publications

segmental flexibility of receptor-bound immunoglobulin e.	the segmental flexibility of mouse immunoglobulin e (ige) bound to its high-affinity receptor on membrane vesicles from rat basophilic leukemia cells was compared to that of ige in solution by measuring the steady-state anisotropy as a function of temperature and viscosity. a monoclonal ige was used to bind the fluorescent probe n-[5-(dimethylamino)naphthalene-1-sulfonyl]-l-lysine (dns-lys) rigidly and specifically in the antigen combining site at the tip of the fab region. the average rotationa ...	1985	2936392
the interaction of recombinant huifn-gamma 1 and recombinant huifn-alpha 2 on the 2-5a system in two distinct cell types.		1985	3008171
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
human papillomavirus types 16 and 18 sequences in early cervical neoplasia.	a total of 100 colposcopic biopsies from patients with abnormal papanicolau's tests were surveyed for the presence of human papillomavirus (hpv) types 16 and 18 sequences by spot-blot hybridization. hpv 16 and 18 dna sequences were detected in 58% of the biopsies. none of the cervical intraepithelial neoplasia grade i (cin i) contained hpv 16 while 50% of the cin iii lesions (carcinoma in situ, cis) contained hpv 16. hpv 18-related sequences were equally represented in cin i, ii, and iii. southe ...	1986	3022464
prevalence and distribution of spotted fever and typhus infections in sierra leone and ivory coast.	a serosurvey for evidence of rickettsial infections was conducted in the rural populations of several tropical rain forest areas in sierra leone and ivory coast. seropositivity rates were surprisingly high in both countries, with more than 7% of the individuals in some districts having antibodies to spotted fever-group rickettsiae. no significant difference was found in the overall prevalence of diagnostic antibody titers to spotted fever-group rickettsiae in sierra leone (5.3%) and ivory coast ...	1986	3025125
antigenic heterogeneity in high- and low-virulence strains of rickettsia rickettsii revealed by monoclonal antibodies.	previously it has been reported that strains of rickettsia rickettsii that differ greatly in their ability to cause disease in guinea pigs are similar by serological and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses. in this study, we used monoclonal antibodies to the virulent r and the relatively avirulent hlp strains to investigate strain differences which might account for the disparate behavior of the strains in guinea pigs. coomassie blue-stained sodium dodecyl sulfate- ...	1986	3080372
ecological study of rocky mountain spotted fever in costa rica.	since rocky mountain spotted fever (rmsf) was recognized in costa rica, ecological studies have been undertaken to discern its distribution and the natural cycle of rickettsia rickettsii. the population of ticks parasitizing animals and vegetation was scarce. rickettsiae were not isolated from pools of ticks nor from spleens of the animals studied. however, the active circulation of rickettsiae was evidenced through the demonstration of spotted fever group antibodies in humans, dogs, and wild ra ...	1986	3080917
actions of antibiotics on rickettsia rickettsii.		1986	3081659
adherence of platelets to human endothelial cells infected by rickettsia rickettsii.	rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, causes widespread vasculitis due to extensive injury of endothelial cells lining small blood vessels. injury to these cells can lead to denudation of the endothelial lining and exposure of the thrombogenic subendothelium. modification of the cell surface, either through minor trauma or as a result of altered cell surface biochemistry, could induce changes reflected in increased platelet reactivity. because membrane modif ...	1986	3081662
humoral immune response to rocky mountain spotted fever in experimentally infected guinea pigs: immunoprecipitation of lactoperoxidase 125i-labeled proteins and detection of soluble antigens of rickettsia rickettsii.	rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, purified from infected l-929 cells by density gradient banding were extrinsically radioiodinated with lactoperoxidase. immunodominant 125i-labeled antigens were identified by radioimmunoprecipitation of detergent-solubilized antigens with protein a-sepharose and anti-r. rickettsii sera collected 0, 3, 7, 11, 32, and 163 days after infection of guinea pigs. the average fever greater than or equal to 40 degrees c was detec ...	1986	3082753
production and characterization of cloned t-cell hybridomas that are responsive to rickettsia conorii antigens.	t-cell hybridomas produced by the fusion of rickettsia conorii immune t cells to the akr thymoma bw 5147 produced interleukin-2 when stimulated with the antigens of three different r. conorii strains. one cloned hybridoma responded only to r. conorii antigens, whereas a second and third cloned hybridoma also responded to the antigens of rickettsia rickettsii sheila smith and rickettsia sibirica 246, respectively. antigen responses required antigen-presenting cells, and this interaction was major ...	1986	3082761
challenging conventional aminoglycoside dosing regimens. the value of experimental models.	although newer antimicrobials look promising for the treatment of serious gram-negative infections, aminoglycosides still remain part of the mainstay of their therapy. traditional intermittent therapy is based upon the premise that high serum aminoglycoside concentrations are toxic. however, the rate of bacterial killing for aminoglycosides is also a concentration-dependent phenomenon. two animal models of pseudomonas pneumonia were utilized to examine the efficacy of non-traditional aminoglycos ...	1986	3089001
the sensitivity of various serologic tests in the diagnosis of rocky mountain spotted fever.	sensitivity indices for various serologic tests in the diagnosis of rocky mountain spotted fever (rmsf) were determined from rmsf surveillance data collected at the centers for disease control (cdc) during 1981-1984. during these 4 years, a total of 4,141 cases of rmsf in the u.s. were reported to cdc. case report forms, which include information on dates and results of serologic testing, were received for 3,567 of these cases; 1,774 were laboratory-confirmed according to criteria published prev ...	1986	3089042
[serologic studies of spotted fever group rickettsiosis occurring in muroto-city, kochi prefecture].		1986	3090165
effects of the binding of myosin light chain kinase on the reactivities of calmodulin lysines.	the effects of the binding of smooth muscle myosin light chain (mlc) kinase on the microenvironments of different regions of calmodulin (cam) were investigated by comparing the acylation rate constants of the seven lysine amino groups of free cam with those of cam complexed with mlc kinase. equimolar amounts of cam and cam-mlc kinase complex were trace labeled with [3h]acetic anhydride in the presence of phenylalanine as a standard nucleophile. after completion of the reaction, equal amounts of ...	1986	3091599
radioimmunoprecipitation of [35s]methionine-radiolabeled proteins of rickettsia conorii and rickettsia rickettsii.		1986	3091709
natural history of rickettsia rickettsii.		1986	3096192
[enterotoxemia in newborn calves due to cl. perfringens types a, c and d].	the clinical symptoms and the morphologic picture of calf enterotoxemia are described. studied were a total of thirty-two dead and slaughtered animals. bacteriologically, the disease was shown to be caused by types a, d, and c of clostridium perfringens. types c and d proved pathogenic for guinea pigs, while type a did not. isolated was a strain of clostridium perfringens, which had high toxigenicity. it was found that calves were fairly often affected with the disease. most severe were the infe ...	1986	2876542
mystacial vibrissae representation within the trigeminal sensory nuclei of the cat.	somatotopic arrangements of axon terminals of primary afferent fibers innervating follicles of the mystacial vibrissae were examined in the cat by the transganglionic horseradish peroxidase (hrp) method. forty to 60 hours after injecting hrp into a single or a group of vibrissal follicles, transported hrp was visualized by the tetramethylbenzidine technique. hrp-labeled axon terminals were distributed in the ventral subnucleus of the principal sensory trigeminal nucleus (ventral vp), in the oral ...	1986	2432098
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
rickettsia rickettsii and rickettsia montana from ixodid ticks in connecticut.	dermacentor variabilis, infected with spotted fever group rickettsiae, parasitized 8 of 70 raccoons captured in newtown, connecticut. the spotted fever agent, rickettsia rickettsii, was isolated and identified from 4 adult d. variabilis and from 1 nymphal ixodes texanus removed from raccoons. this verifies the presence of this etiologic agent in ticks in an area where 6 people had clinical signs and symptoms of rocky mountain spotted fever (rmsf) and antibodies to r. rickettsii. these are the fi ...	1986	3946737
seroepidemiology of campylobacter pyloridis.	a total of 1158 sera were tested by an enzyme linked immunosorbent assay (elisa) for igg and iga antibodies to campylobacter pyloridis. when used to test sera from gastroendoscopy patients this method had a sensitivity of 91% and a specificity of 75%. ninety-eight of 108 infected patients (91%) were seropositive whereas only 23 of 92 (25%) uninfected patients were seropositive (p less than 0.001). positive serology was also associated with histological gastritis (p less than 0.001). when sera fr ...	1986	3463897
renal cell carcinoma in the wistar-lewis rat: a model for studying the mechanisms of cholesterol acquisition by a tumor in vivo.	renal adenocarcinoma implanted into isogeneic wistar-lewis rats closely resembles human renal cancer. this paper characterizes the tumor's growth rate, metastatic potential, and its light and electron microscopic appearance. additionally, for the first time, the pathways through which a tumor acquires the cholesterol needed for growth were quantified in vivo. two 1-mg pieces of renal carcinoma were implanted beneath the renal capsule of 80 wistar-lewis rats. of the implanted tumors 95% "took" an ...	1986	3486039
prevention of platelet alloimmunization.	based on transfusion studies in patients and animal model systems, there are currently only two established methods of at least delaying platelet alloimmunization. these involve the administration of leukocyte-poor platelets and red cells or reducing a recipient's exposure to incompatible donor antigens by using single-donor pheresis products. the application of these approaches is limited by practical considerations of achieving sufficiently leukocyte-poor blood products to produce the desired ...	1986	3515365
production of colicin e5 by epidemic strains of salmonella typhimurium var. copenhagen.	sixty eight strains of salmonella typhimurium were isolated during an outbreak of salmonellosis in the paediatric ward of saadi hospital in shiraz, iran. eleven of the strains were shown to produce a single type of colicin of group e. this colicin was identified as colicin e5. most of the colicinogenic strains exhibited similar drug sensitivity and phage typing patterns and all were of the serotype s. typhimurium var. copenhagen.	1986	3526095
suppression of migration inhibition factor (mif) production by mitomycins in vitro.	the effects of a series of mitomycins in a modified version of the migration inhibition factor (mif) test were investigated. this modified assay, which uses monocytes of the mouse cell line wehi3 as target cells for mif, discriminates between effects on the migrating monocytes, the production of mif by lymphocytes and the activity of the produced mif. the 7-methoxy mitomycins were found to be more active than the 7-amino and 7-hydroxy derivatives in suppressing both the spontaneous migration of ...	1986	3533763
suppression of migration inhibition factor (mif) production by mitomycins in vitro.	the effects of a series of mitomycins in a modified version of the migration inhibition factor (mif) test were investigated. this modified assay, which uses monocytes of the mouse cell line wehi3 as target cells for mif, discriminates between effects on the migrating monocytes, the production of mif by lymphocytes and the activity of the produced mif. the 7-methoxy mitomycins were found to be more active than the 7-amino and 7-hydroxy derivatives in suppressing both the spontaneous migration of ...	1986	3533763
multiplication-stimulating activity-induced alkalinization of canine renal proximal tubular cells.	the actions of a variety of polypeptide growth factors on isolated cells are thought to be initiated by stimulation of na+-h+ exchange across the plasma membranes of the cells resulting in intracellular alkalinization. to determine whether insulin-like growth factors (igfs) exert actions through such a mechanism, we incubated suspensions of canine renal proximal tubular segments with insulin or igf i or with multiplication-stimulating activity (msa)/igf ii. changes in intracellular ph were detec ...	1986	3533920
propionibacterium acnes cerebral botryomycosis. the role of plastic embedding in the diagnosis of grain-producing infections.	botryomycosis is a chronic infection in which bacterial colonies, or "grains," occur in purulent exudate associated with draining sinus tracts or abscess formation. this condition usually is caused by staphylococcus and streptococcus, but other organisms occasionally have been implicated. this report describes the first case of botryomycosis caused by propionibacterium acnes, a diphtheroid that has been rarely associated with opportunistic disease. in this case, plastic embedding allowed the obs ...	1986	3535486
studies on the mechanics of the normal human middle ear.	the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...	1986	3536194
infection and injury of human endothelial cells by rickettsia rickettsii.		1986	3122642
manifestations of kingella kingae infections in adults: resemblance to neisserial infections.	kingella kingae is a rare human pathogen. most reported infections are in children and involve endocardium, vascular space, and skeletal tissues. we report herein two cases of k. kingae infection recently seen in adults. kingella kingae caused acute meningitis in a patient with sickle cell anemia and in the second patient with alcoholic liver disease, sepsis with a petechial rash. the clinical presentation due to k. kingae closely resembled that caused by related neisseria genus.	1986	3709097
bacterial esophagitis in immunocompromised patients.	we studied the clinical and pathologic features of bacterial esophagitis in three index cases identified by endoscopic biopsy and in 20 autopsy cases. fourteen of the 23 patients had malignant hematologic conditions, aplastic anemia, or solid tumors; ten were profoundly neutropenic (white blood cell count, less than 100/mm3 [less than 0.1 x 10(9)/l]). the organisms involved in bacterial esophagitis were gram-positive cocci in 14, gram-negative bacilli in three, mixed gram-negative bacilli and gr ...	1986	3718132
sensitive high-performance liquid chromatographic determination of pirenzepine in plasma.	a high-performance liquid chromatographic method for the determination of pirenzepine in human plasma is reported using imipramine as an internal standard. the assay has a lower limit of detection of 2.5 ng/ml. the calibration function is found to be linear in the range from 5 ng/ml up to at least 100 ng/ml. two sets of chromatographic conditions are described, which provide different chromatographic selectivities for the separation of the compounds of interest from other material present in a s ...	1986	3754561
[acute campylobacter jejuni enterocolitis in children under 2 years of age].		1986	3764191
[effect of a helminth invasion on the human staphylococcal carrier state].		1986	3796454
herpes simplex virus: a role in the aetiology of alzheimer's disease?		1986	3005513
a serological survey of scrub, tick, and endemic typhus in sabah, east malaysia.	a seroepidemiological survey of 837 people and 383 febrile patients was performed in rural areas of sabah. we determined that the rickettsial diseases scrub typhus and endemic typhus were uncommon causes of febrile illness, as was tick typhus, except in forest dwelling peoples. the rate of occurrence of sfgr specific antibody was 16.5% among 412 forest dwellers, indicating that tick typhus may be a frequent cause of illness in this population.	1986	3107139
computed tomography of intracerebral gangliogliomas.	ct and clinical findings in 6 histologically proven cases of intracerebral gangliogliomas (out of 14,000 cranial ct examinations) are presented. the tumor is most often isodense, with areas of calcification. even though ct findings are nonspecific, they are suggestive of the benign nature of the tumor. when this is histologically confirmed (by biopsy) the more aggressive forms of treatment may be obviated.	1986	262206
kinetics of carboxymyoglobin and oxymyoglobin studied by picosecond spectroscopy.	picosecond studies of carboxymyoglobin (mbco) and oxymyoglobin (mbo2) reveal that excitation at 530 nm induces photodissociation at less than 8 ps. the kinetic and structural changes were monitored by following absorbance changes at selected wave-lengths in the soret (b) band and in the q band. within the 10 ps-0.45 ns period of time over which our experiments were conducted, the absorbance changes in the soret and q bands for mbco and mbo2 correspond to the conventional long-term, steady-state ...	1986	262399
[tough project to get activities on working environment started].		1987	260417
[clinical criteria in the choice of surgical methods in the treatment of mandibular prognathism in the light of own observations].		1987	265851
sequence analysis of the 17-kilodalton-antigen gene from rickettsia rickettsii.	dna obtained from the sheila smith strain of rickettsia rickettsii was digested to completion with the restriction endonucleases bamhi and sali and ligated with the plasmid vector puc19. the ligation mixture was used to transform escherichia coli. a total of 465 bacterial clones were screened for antigen production with hyperimmune rabbit serum. one of the reactive clones, containing a recombinant plasmid designated pss124, was solubilized and subjected to immunoblot analysis and revealed expres ...	1987	3108232
steroid production in vitro by granulosa, theca, and luteal cells from goat ovaries.	basal progesterone (p4) production by isolated goat ovarian cells in vitro was in the order corpus luteum (cl) greater than granulosa (g) greater than theca (th), while estradiol (e2) production was in the order th greater than g greater than cl. in g cells, various concentrations (0.01 to 100 micrograms/ml) of luteinizing hormone (lh), human chorionic gonadotropin (hcg) and follicle-stimulating hormone (fsh) increased p4 and e2 secretion. testosterone (t, 10(-9) to 10(-5) m) produced dose-depen ...	1987	3109512
antibodies to spotted fever-group rickettsiae in dogs in north carolina.	a seroepidemiologic survey was conducted to determine the prevalence of antibodies reactive with 4 spotted fever-group (sfg) rickettsiae in sera of dogs from various geographic regions in north carolina. serum specimens were obtained from 600 dogs, and antibody titers were determined, using microimmunofluorescence. data analysis (setting as the criterion for a positive result, a rickettsia rickettsii titer greater than or equal to 1:64) overestimated the actual prevalence of canine exposure to t ...	1987	3118744
effects of ouabain on cytosolic calcium in lymphocytes, platelets and adrenomedullary cells.	it has been suggested that a circulating inhibitor of na/k atpase can stimulate natriuresis and cause vasoconstriction in essential hypertension by stimulating transmembrane na/ca exchange to produce increased cytosolic concentrations of ionized calcium ([ca++]i) in renal tubular and arteriolar smooth muscle cells. if this inhibitor affected [ca++]i in all cell types, then clinical assays for its presence could be applied to easily accessible cells such as blood cells or platelets, and the inhib ...	1987	3429864
coagulase-negative staphylococci resistant to beta-lactam antibiotics in vivo produce penicillin-binding protein 2a.	strains of coagulase-negative staphylococci were tested for in vivo resistance in a rabbit model of prophylaxis of endocarditis. regimens of nafcillin, cefazolin, cefamandole, and vancomycin were compared for efficacy in the prevention of infection caused by two methicillin-resistant strains and a susceptible strain. for the two resistant strains, vancomycin was the most effective drug tested. all regimens were effective against the susceptible strain. the two strains for which prophylaxis with ...	1987	3439802
perturbation of differentiated functions in vivo during persistent viral infection. iii. decreased growth hormone mrna.	lymphocytic choriomeningitis virus (lcmv) persistent infection that results from the inoculation of c3h/st newborn mice causes growth hormone (gh) deficiency and associated disease characterized by both reduced weight and serum glucose levels. molecular analysis of pituitary nucleic acids shows gh deficient mice have, on average, fivefold reduced levels of gh mrna although the histopathology of such gh producing cells is normal. northern blots indicate that the length of gh mrna is comparable in ...	1987	3811236
vasoactive intestinal polypeptide immunoreactive neurons in the primary visual cortex of the cat.	when cat visual cortex (area 17) is reacted with an antibody to vasoactive intestinal polypeptide (vip) a variety of neuronal types is labelled. many of the labelled neurons are bipolar in form and are most common in layers ii and iii, although significant numbers of bipolar neurons are also encountered in layer v. multipolar cells are also labelled. these are most frequent in layer iv and have a variety of shapes. in layer i, the labelled cells are of three varieties, i.e. horizontal bipolar ce ...	1987	3295123
subfractionation of the dansylated derivatives of glucosyl galactosyl hydroxylysine by liquid chromatography and its application to a specific alpha-1,2-glucosidase assay.	the dansyl derivative of glucosyl galactosyl hydroxylysine (ggh) was separated into two components, as gp-i (monodansyl ggh) and gp-ii (didansyl ggh) by paper chromatography. gp-i was further fractionated into four peaks (a, b, c and d) by reversed-phase liquid chromatography. these peaks corresponded to the dansyl derivatives at the alpha-amino (a and b) and epsilon-amino (c and d) groups of their hydroxylysine residues. there is the possibility that the fractions for b and d are diastereoisome ...	1987	3298291
phenotypic composition and in vitro functional capacities of unmodified fresh cells infiltrating acutely rejected human kidney allografts.	cell surface markers of isolated graft-infiltrating cells (gic) were studied, and functional in vitro assays performed in 8 cases of acute irreversible rejection of human renal allografts. the gic were mostly activated t cells (okt11+, okt3+, ia+), with predominance of the cytotoxic/suppressor t cell phenotype (okt8+). a small proportion of b cells and monocytes/macrophages were also present among these gic. the gic were able to proliferate with lectin of allogeneic stimulation and were strongly ...	1987	3299920
a human teratocarcinoma which expresses a rare neuronal cell surface antigen.	a molecular characterization of the events at the cell surface of neurons is pivotal for our understanding of how the nervous system is formed and maintained. this study of cell surface events in the human nervous system may be crucial to the study of human neurological maladies. ntera-2 is a human teratocarcinoma which is unique amongst the teratocarcinomas for its ability to express many neurons. tested for the binding of a monoclonal antibody tor 23, which recognizes a surface antigen on rare ...	1987	3315107
antibodies against mammary derived growth inhibitor (mdgi) react with a fibroblast growth inhibitor and with heart fatty acid binding protein.	a polypeptide growth inhibitor, designated as mammary derived growth inhibitor, has previously been purified from lactating bovine mammary glands. polyclonal rabbit antiserum raised against mammary derived growth inhibitor cross-reacts with bovine heart fatty acid binding protein and bovine peripheral myelin p2 protein. these results are consistent with the observation that the amino acid sequence of mammary derived growth inhibitor showed homology to the sequences of these proteins. in a parall ...	1987	3318832
hormones in milk.		1987	3320320
insulin resistance and abnormal ovarian responses to human chorionic gonadotropin in chronically anovulatory women.	we studied the interrelationships between insulin resistance, obesity, and abnormal ovarian androgen secretion in chronically anovulatory women with clinical or biochemical evidence of hyperandrogenism. four groups of six subjects each were studied: (1) normal weight (within 10% ideal body weight) anovulatory, (2) obese (greater than 120% ideal body weight) anovulatory, (3) normal weight eumenorrheic, and (4) obese eumenorrheic. after dexamethasone suppression, human chorionic gonadotropin (2000 ...	1987	3322017
effect of intestinal factors on extraction of insulin in perfused rat liver.	to study the direct effect of intestinal factors on hepatic extraction of insulin, an investigation was made into the extraction of insulin from isolated rat liver perfused with portal venous effluent (pve) obtained from the isolated perfused rat intestine. rat intestine was perfused with krebs-ringer bicarbonate medium for 45 min, and the pve was collected from glucose-, lipid-, or nacl-treated and untreated control intestines. the pve, after adjustment of its glucose (180 mg/dl) and insulin (2 ...	1987	3322037
high mutagenic activity of 3-azido-1,2-propanediol (azidoglycerol, ag) in strain d7 of saccharomyces cerevisiae.	3-azido-1,2-propanediol (azidoglycerol, ag) showed a high mutagenicity in strain d7 of saccharomyces cerevisiae. at 5 mm it increased the spontaneous frequency of isoleucine revertants 3500 times and the frequency of gene convertants 3000 times during 24 h of growth, reducing the growth rate to 30%. in non-growth conditions, treatment with 150 mm of ag for 3 h reduced cell survival to 60% and enhanced the frequency of isoleucine revertants 490 times and tryptophan-independent convertants 50 time ...	1987	3553915
rhodopsin, vitamin a, and interstitial retinol-binding protein in the rd chicken.	in order to determine whether blindness in the rd strain of rhode island red chickens is due to a defect in the vitamin a (visual) cycle, spectroscopy, high performance liquid chromatography, and immunochemical techniques were used to compare the amounts of rhodopsin, interstitial retinol-binding protein, and vitamin a compounds in the dark-adapted eyes of homozygous rd and heterozygous carriers. in both groups of chickens, (up to 6 weeks post-hatching) the distribution of stored vitamin a diffe ...	1987	3557867
[effects of azelastine on an experimentally-induced acute hepatic failure model].		1987	3606396
the effect of corneal contact lenses on the oxygen tension in the anterior chamber of the rabbit eye.	the oxygen tension in the aqueous humor in the anterior chamber of rabbit eyes was measured continuously with a polarographic electrode. the normal oxygen tension in the anterior chamber was 23 +/- 2 mm hg (mean +/- sd, n = 4). a contact lens was then placed on the cornea for at least 10 min and the drop in oxygen tension recorded. a hard polymethylmethacrylate lens reduced the oxygen tension by 16 +/- 4 mm hg, and a larger hydroxyethylmethacrylate soft lens (soflens) decreased oxygen tension by ...	1987	3654146
tuberculin response of sri lankan children after bcg vaccination at birth.	a total of 740 healthy children aged between 3 months and 11 years who had received bcg vaccination in the first month of life were mantoux tested in sri lanka. despite 97% having a visible scar following vaccination, 80% showed tuberculin anergy (0-1 mm). those without a scar showed no response. there was no correlation between scar size and the mantoux response. a low mean mantoux reaction was seen at all ages: 3.5 mm at 3 months, 3.2 mm at 18 months, 1.8 mm at 5-7 years and 1.9 mm at 9-11 yea ...	1987	3660460
ethyl acrylate distribution, macromolecular binding, excretion, and metabolism in male fisher 344 rats.	we have demonstrated previously that ethyl acrylate causes severe acute forestomach (nonglandular portion of the stomach) toxicity in rats. ethyl acrylate was also shown to cause forestomach tumors when administered to rats chronically by gavage. the current studies were designed to investigate ethyl acrylate distribution, excretion, and metabolism, as well as the macromolecular interactions of ethyl acrylate (etac) in the forestomach (target organ) and liver (nontarget organ). 2,3-[14c]ethyl ac ...	1987	3691998
ethyl acrylate distribution, macromolecular binding, excretion, and metabolism in male fisher 344 rats.	we have demonstrated previously that ethyl acrylate causes severe acute forestomach (nonglandular portion of the stomach) toxicity in rats. ethyl acrylate was also shown to cause forestomach tumors when administered to rats chronically by gavage. the current studies were designed to investigate ethyl acrylate distribution, excretion, and metabolism, as well as the macromolecular interactions of ethyl acrylate (etac) in the forestomach (target organ) and liver (nontarget organ). 2,3-[14c]ethyl ac ...	1987	3691998
neutralizing activity of monoclonal antibodies to heat-sensitive and heat-resistant epitopes of rickettsia rickettsii surface proteins.	antiprotein monoclonal antibodies derived from mice inoculated with rickettsia rickettsii heated at 56 degrees c for 15 min are of two types: one is type specific for epitopes denatured by moderate temperatures, and the other is specific for epitopes resistant to 100 degrees c for 5 min. the heat-resistant epitopes are found by immunoblotting on multiple polypeptides after solubilization of the rickettsiae at temperatures of 56 degrees c or higher. most, but not all, antibodies to the heat-sensi ...	1987	2434430
intrathecally administered apomorphine or ly171555 reduces nociceptive responses recorded from ventrobasal thalamic neurones in urethane anaesthetised rats.	the effects of intrathecal injections of the d2 agonists apomorphine and ly171555 were studied on responses of ventrobasal neurones responding to noxious stimulation in the urethane anaesthetised rat. at a dose of 75 micrograms/kg the d2 agonists had little effect on a total of 18 thalamic neurones. a higher dose (100 micrograms/kg) produced a reversible reduction of thalamic nociceptive responses on 15 occasions. a transient fall in blood pressure usually accompanied the intrathecal injection o ...	1987	2953995
reactivity of monoclonal antibodies to rickettsia rickettsii with spotted fever and typhus group rickettsiae.	analysis of 15 spotted fever group (sfg) and 2 typhus group strains of rickettsiae with a panel of monoclonal antibodies revealed a number of shared and unique epitopes of the 120- and 155-kilodalton surface proteins. all of the sfg strains but neither of the typhus group strains reacted with antibody to the lipopolysaccharidelike antigen of rickettsia rickettsii; possibly the lipopolysaccharidelike antigen is the common antigen which defines the sfg. north carolina and montana strains of r. ric ...	1987	2432081
in situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.	we have developed an in situ hybridization assay capable of detecting enteroviral rna in myocardial cells, using molecularly cloned coxsackievirus b3 cdna as a diagnostic probe. because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group a and b coxsackieviruses and the echoviruses, detection of these various agents commonly implicated in human viral heart disease is possible in a single hybridization assay. we demonstrate the consid ...	1987	2819870
subcellular localization of enzymes in streptomyces aureofaciens and its alteration by benzyl thiocyanate. i. phosphatases and atp-glucokinase.	mycelia of a low- and a high-production strain of streptomyces aureofaciens were converted into protoplasts and divided into five subcellular fractions in order to localize exopolyphosphatases (ec 3.6.1.11), triphosphatase (ec 3.6.1.25), inorganic diphosphatase (ec 3.6.1.1), apyrase (ec 3.6.1.5) and glucokinase (ec 2.7.1.2). the highest specific activity of enzymes hydrolyzing polyphosphates was found in cytoplasmic vesicles and membranes. triphosphatase was detected in the periplasmic fraction. ...	1987	2826319
cloned gene of rickettsia rickettsii surface antigen: candidate vaccine for rocky mountain spotted fever.	two major protective antigens of rickettsia rickettsii have been previously described. in this study, we cloned the gene encoding one of these antigens into escherichia coli and tested the effectiveness of the recombinant-made product as a vaccine for rocky mountain spotted fever. a clone bank of r strain r. rickettsii dna was made in e. coli k-12 by using the plasmid vector pbr322. transformants were screened for their ability to make rickettsial antigens by reactivity with rabbit antibodies to ...	1987	3099387
in vitro antibiotic susceptibility of rickettsia rickettsii and rickettsia conorii: plaque assay and microplaque colorimetric assay.		1987	3104481
susceptibility of rickettsia conorii and r. rickettsii to pefloxacin, in vitro and in ovo.	the activity of pefloxacin against rickettsia conorii and r. rickettsii was determined by several methods. the mean survival time of embryonated eggs infected with r. conorii was increased by pefloxacin 50 micrograms/egg; plaque formation in vero cells was inhibited by 1 mg/l. in a microplate assay, the mic of pefloxacin was 0.5 mg/l for r. conorii and 1 mg/l for r. rickettsii. the results support the use of pefloxacin in treating spotted fever rickettsioses.	1987	3106303
viral specificity of multiple sclerosis tear immunoglobulins.	the mucosal immune system plays an important role in determining how an individual handles infection by most of the common viruses. tears form part of this system and participate in mucosal immunity. we examined tears from multiple sclerosis (ms) and control subjects for antibodies to seven common neurotropic viruses. antibodies to multiple viruses were detected in ms tears, especially when tears were obtained from chronic progressive active patients. in particular, ms patients showed a signific ...	1987	3029176
[effect of cholera enterotoxin on carbohydrate metabolism of the liver and small intestine mucosa in the rabbit].	changes in carbohydrate metabolism were studied in the isolated intestinal loops of rabbits during secretory diarrhea, induced by cholera enterotoxin. glucose synthesis level in the small intestinal mucosa and liver was measured by isotope technique, using l-alanine as a precursor. intestinal gluconeogenesis, calculated per mg of protein, appeared to be twice higher than in the liver of fasting rabbits. cholera enterotoxin administration enhanced gluconeogenesis in the liver by 60%, as compared ...	1987	3032299
molecular cloning of cdnas of human liver and placenta nadh-cytochrome b5 reductase.	a cdna coding for human liver nadh-cytochrome b5 reductase (cytochrome b5 reductase, ec 1.6.2.2) was cloned from a human liver cdna library constructed in phage lambda gt11. the library was screened by using an affinity-purified rabbit antibody against nadh-cytochrome b5 reductase of human erythrocytes. a cdna about 1.3 kilobase pairs long was isolated. by using the cdna as a probe, another cdna (pb5r141) of 1817 base pairs was isolated that hybridized with a synthetic oligonucleotide encoding p ...	1987	3035541
the release of pituitary lh and sprouting of lh-releasing hormone (lhrh)-containing neurons after anterior hypothalamic deafferentation.	the chronology of changes in plasma luteinizing hormone (lh) and the distribution of immunoreactive neuronal processes containing lh releasing hormone (lhrh-ir) were studied in the female rat after surgical interruption of anterior neural connections of the mediobasal hypothalamus (mbh). spontaneous lh surges on the afternoon of proestrus and lh release after estradiol benzoate (eb) followed 48 h later by progesterone (p) administration were studied in ovariectomized (ovx) rats. the maximum incr ...	1988	3044516
characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain.	antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ...	1988	3055210
[experimental study on liver cancer--effect of intra-arterial injection of adriamycin-lipiodol emulsion on vx2 liver tumor].		1988	2846718
molecular biological characterization of equine herpesvirus type 1 (ehv-1) isolates from ruminant hosts.	isolates of equine herpesvirus type 1 (ehv-1) originating from affected antelope and cattle were compared with reference ehv-1 and ehv-4 isolates and were characterized. based on cross-neutralization, dna restriction profiles and blot-hybridization data these isolates could be characterized as ehv-1. one isolate (from an antelope) with a different restriction profile showed significant dna homology with ehv-1, partial homology with ehv-4, and little or no homology with ehv-2 and hsv-1 dnas. blot ...	1988	2849252
a prospective study of platelets and plasma proteolytic systems during the early stages of rocky mountain spotted fever.	we prospectively examined early changes in platelets and plasma proteolytic systems in 12 vaccinated and 6 unvaccinated volunteers in whom rocky mountain spotted fever developed after challenge with rickettsia rickettsii. the platelet counts declined while the plasma concentration of beta-thromboglobulin and the ratio of beta-thromboglobulin to platelet factor 4 increased, indicating in vivo activation of platelets. plasma levels of antithrombin iii decreased and levels of fibrinopeptide a incre ...	1988	2965302
protective monoclonal antibodies recognize heat-labile epitopes on surface proteins of spotted fever group rickettsiae.	thirty-eight monoclonal antibodies that have not been reported previously were developed from mice immunized with rickettsia rickettsii, r. conorii, and r. sibirica. western immunoblotting showed that these monoclonal antibodies are directed against heat-sensitive epitopes which are located on two major surface polypeptides with molecular sizes ranging from 115 to 150 kilodaltons. the detection of the two bands did not depend on the presence of 2-mercaptoethanol. both bands were destroyed by tre ...	1988	2458318
hematology, intestinal parasites, and selected disease antibodies from a population of bobcats (felis rufus) in central arkansas.	eight bobcats with adjoining or overlapping home ranges were examined. hematological values were within previously reported ranges. six bobcats demonstrated antibody titers to toxoplasma gondii. isospora spp., taenia taeniaeformis, spirometra mansoides, physoloptera rara, toxocara cati, strongyloides spp., trichurus spp., capillaria spp., and ancyclostoma spp. were found also in the animals examined. the mean number of parasite species per host was 4.1. all bobcats tested negative for serum anti ...	1988	3352091
anion transport during maturation of erythroblastic cells.	bromide uptake was measured in single maturing erythroblastic cells of rabbits by means of x-ray microanalysis. increase in bromide uptake as the cells matured was observed. the order of cells from low to high bromide uptake was: early erythroblast less than late erythroblast less than marrow red cells less than peripheral red blood cells. the transition from low to high bromide uptake is correlated to the accumulation of iron which begins in the late erythroblast. a decrease in rubidium uptake ...	1988	3367365
beef and chocolate: a partial reprieve.		1988	3375809
[effect of acute and chronic administration of melatonin on the temporal dynamics of apomorphine-induced stereotypy].	melatonin (1-20 mg/kg) weakened in a dose-dependent way the intensity degree of apomorphine-induced stereotypy in rats. according to the spectral analysis data, this is followed by an increase of the number of waves with a short (2-3 per min) period in the temporal structure of stereotypy recorded every one minute. after administration of melatonin (1 mg/kg), the effect of apomorphine changed more in the afternoon (1-3 p.m.) than in the morning (6-8 a.m.) or the evening (6-8 p.m.) hours. the act ...	1988	3378603
the phagocytic activity of lumbricus terrestris leukocytes is enhanced by the vertebrate opsonins: igg and complement c3b fragment.	the effect of the vertebrates opsonins: igg and complement c3 fragments on phagocytic activity of lumbricus terrestris leukocytes towards sheep erythrocytes was studied. sheep erythrocytes were previously sensitized with specific igg or igm and coated with one of the third component fragments (c3b, c3bi, c3d) of human complement. our results show that leukocyte phagocytosis was enhanced by vertebrate igg and c3b complement fragments but not by igm and fragment c3d. because opsonization in verteb ...	1988	3384156
occupational factors and the incidence of cancer of the bladder in canada.	during 1979-82, a case-control study of occupational factors and urinary bladder cancer was conducted in edmonton, calgary, toronto, and kingston, canada. a total of 826 histologically verified cases of cancer were individually matched by sex, age, and area of residence to 792 randomly selected population controls. subjects were specifically asked about employment in several industries thought relevant to risk of bladder cancer. information was also obtained on lifelong occupational history, wit ...	1988	3395572
pseudomonas aeruginosa sepsis presenting as peripheral subcutaneous nodules. report of 2 cases.	two patients developed disseminated subcutaneous nodules with febrile illness. in both cases, pseudomonas aeruginosa was isolated from the lesions; blood cultures yielded the same organism in one case, and were negative in the other. the portal of entry was thought to be a jugular hemodalysis catheter in the first case and a necrotic zoster complicating lymphoma in the second case. both patients' condition improved with antibiotic therapy and the pseudomonas nodules resolved without surgical dra ...	1988	3403778
comparative bioavailability to humans of ascorbic acid alone or in a citrus extract.	this study was performed to determine whether synthetic ascorbic acid (aa) alone or in a natural citrus extract containing bioflavonoids, proteins, and carbohydrates was more bioavailable to human subjects. the effect of a single 500-mg ascorbate dose of the two forms and a placebo citrus extract on plasma ascorbate was examined in eight fasting subjects. a comparison of the areas under the plasma concentration-time curves showed that the citrus extract was 35% more absorbed than aa (p less than ...	1988	3414575
clinical, hematologic, and humoral immune response in female dogs inoculated with rickettsia rickettsii and rickettsia montana.	female beagles were inoculated intradermally with a sublethal dose of rickettsia rickettsii and r montana. three dogs (group 1) were inoculated with 2 x 10(2) plaque-forming units (pfu) of r rickettsia and were treated with tetracycline beginning on postinoculation day (pid) 12; 3 dogs (group 2) were inoculated with 2 x 10(2) pfu of r rickettsii but were not treated; 3 dogs (group 3) were inoculated with 2 x 10(2) pfu of r montana. group-3 dogs failed to seroconvert and were inoculated a second ...	1988	3128147
limited proteolysis of bovine adrenodoxin reductase: evidence for a domain structure.	treatment of bovine adrenodoxin reductase with trypsin under conditions of limited proteolysis yields two major fragments of apparent molecular weights 30,500 and 20,200. the fragments, which have been partially purified by affinity chromatography to remove most of the intact adrenodoxin reductase, retain adrenodoxin-dependent nadph cytochrome c reductase activity. kinetic analyses yield vmax and km (adrenodoxin) values of 485 min-1 and 0.96 microm, respectively, at an ionic strength of 0.13 m i ...	1988	3128975
[experimental chemo- and chemoendocrine therapy of human breast carcinomas serially transplanted into nude mice].	four human breast carcinoma strains serially transplanted into nude mice were used for the experimental chemotherapy and combination chemoendocrine therapy. whereas three of these strains (mcf-7, br-10, tm-61) possessed cytosol estrogen receptor (erc) and were dependent on estradiol for the tumor growth, the other strain (mx-1) without erc was hormone independent. for the chemotherapy, mitomycin c (mmc), adriamycin (adm), cyclophosphamide (cpm) and 5-fluorouracil (5-fu) were administered 3 times ...	1988	3129645
in vitro evaluation of josamycin, spiramycin, and erythromycin against rickettsia rickettsii and r. conorii.	the antimicrobial activities of josamycin, erythromycin, and spiramycin against rickettsia conorii and r. rickettsii were evaluated in two tests: a dye-uptake assay and a plaque assay. the mic of josamycin was 1 microgram/ml for both species; the mics of erythromycin and spiramycin were 4 to 8 and 16 to 32 micrograms/ml, respectively, for both species. only josamycin may be of clinical use in treating spotted fever rickettsiosis. it may be useful in treating pregnant women and young children.	1988	3129987
a focus of rocky mountain spotted fever within new york city.	in the spring and summer of 1987, four persons acquired rocky mountain spotted fever within new york city, an area in which the disease had not previously been known to be endemic. three of the four patients were residents of the soundview area of the bronx. all diagnoses were confirmed by indirect fluorescent-antibody tests. environmental investigation revealed that the tick vector for rickettsia rickettsii, dermacentor variabilis, was present in a local park. of the 66 specimens of d. variabil ...	1988	3130574
protection of guinea pigs from experimental rocky mountain spotted fever with a cloned antigen of rickettsia rickettsii.		1988	3134494
serological examinations of slaughtered animals (cattle and goats) in the slaughter-house of colombo/sri lanka towards antibodies against brucellosis, q-fever, rmsf-rickettsia-group, listeriosis, echinococcosis and adenoviruses.	serological examinations of slaughtered animals (76 cattle and 184 goats) in the slaughter-house of colombo/sri lanka revealed antibodies against listeriosis, brucellosis, q-fever, rmsf-rickettsia-group, echinococcus and adenoviruses. as the results show, part of this zooanthroponosis has a role to play in human medicine and human infections are to be expected.	1988	3139493
expression of the gene encoding the 17-kilodalton antigen from rickettsia rickettsii: transcription and posttranslational modification.	recently, we reported the molecular cloning and nucleotide sequence analysis of a gene from rickettsia rickettsii that codes for a 17-kilodalton antigen (17k antigen) and is preceded by sequences closely resembling the -10 and -35 consensus sequences for recognition by escherichia coli rna polymerase (anderson et al., j. bacteriol. 169:2385-2390, 1987). experiments described in this report indicate that the start sites for initiating transcription of the 17k antigen gene are identical in the e. ...	1988	3139629
potential for free radical-induced lipid peroxidation as a cause of endothelial cell injury in rocky mountain spotted fever.	cells infected by rickettsia rickettsii, the causative agent of rocky mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. these changes are consistent with those that might be expected to occur following peroxidation of membrane lipids initiated by oxygen radical species, such as the hydroxyl radical or a variety of organic radicals. using a fluorescent probe, we have fou ...	1988	3141280
extraction of protective components of rickettsia rickettsii with n-octyl-beta-d-glucopyranoside.	two rickettsia rickettsii proteins, with apparent molecular masses of 120 and 155 kilodaltons, have been implicated as important antigens in immunity to rocky mountain spotted fever. an n-octyl-beta-d-glucopyranoside-soluble fraction of r. rickettsii was found to be enriched for these proteins along with others. vaccination of mice with the detergent-soluble fraction protected them against death caused by r. rickettsii and protected guinea pigs from developing rocky mountain spotted fever after ...	1988	3142019
antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group rickettsiae in louisiana dogs.	antibodies to ehrlichia canis, ehrlichia platys, and spotted fever group (sfg) rickettsiae were detected by indirect immunofluorescence in sera from 27 ill individually owned thrombocytopenic dogs (platelet concentrations less than 200,000 platelets/microliters) and 59 healthy kenneled dogs located in southern louisiana. platelet concentrations less than 100,000 platelets/microliters were detected in 63% of ill thrombocytopenic dogs and 6.8% of healthy kennel dogs. one ill thrombocytopenic dog h ...	1988	3146636
the population dynamics of two vertically transmitted infections.	the transmission of keystone virus in the mosquito aedes atlanticus and of rickettsia rickettsii in the tick dermacentor andersoni is modeled and analyzed. both of these infections can be transmitted vertically from an infective parent to newborn offspring as well as horizontally via direct or indirect contacts with infected individuals. the vertical transmission mechanism plays a major role in the maintenance of these infections and its effects are analyzed in detail. this same mechanism can ac ...	1988	3148206