Publications

a monoclonal antibody reacts with maltose-binding protein of escherichia coli and related enteric bacteria.	maltose-binding protein (mbp) encoded by male is essential for the energy-dependent translocation of maltose through the cytoplasmic membrane of bacteria. its property of specific binding to maltose has been used in constructing fusion proteins for easy affinity purification. a monoclonal antibody named mab sc1d7 was produced against escherichia coli mbp. this mab also bound to mbp-containing recombinant proteins in both western blotting and immunoprecipitation analysis. as a result, this mab ca ...	1997	9212834
incidence and clinical symptoms of aeromonas-associated travellers' diarrhoea in tokyo.	in a survey examining the causes of travellers' diarrhoea treated in tokyo between july 1986 and december 1995, aeromonas species were isolated from 1265 (5.5%) of 23,215 travellers returning from developing countries. aeromonas species were the fourth most frequent enteropathogen isolated, following enterotoxigenic e. coli (8.5%), salmonella spp. (7.6%) and plesiomonas shigelloides (5.6%). aeromonas species were found in 1191 (5.6%) of 21,257 patients with diarrhoea and in 74 (3.8%) of 1958 hea ...	1997	9363009
[studies on bacillary dysentery cases of overseas travellers--during 1979 to 1995].	a total of 36,780,440 overseas travellers during 1979-1995 (17 years) were quarantined at osaka and kansai airport quarantine station, 84,777 travellers reported themselves suffer from diarrhoea. stools from 29,587 persons were bacteriologically examined. various enteropathogenic bacteria were isolated from 9,766 (33.0%) patients of the stools examined. isolated species were as follows: plesiomonas shigelloides (3,234 cases); salmonella spp. (2,236 cases); enterotoxgenic escherichia coli (1,621 ...	1997	9391324
[prevalence of plesiomonas shigelloides in surface water].	plesiomonas shigelloides is a gram negative rod that has been implicated in acute gastroenterities, meningitis, bacteremia and osteomyelitis. is normally found in the environment, in fresh water, fish and birds, particularly in tropical and subtropical regions. the present study was pursued with the purpose of establish the role of surface water as reservoir and transmission vehicle and to evaluate the seasonal effect on incidence. surface water samples were isolated on the surface of two select ...	1997	9429641
conformational stabilization of the altruronic acid residue in the o-specific polysaccharide of shigella sonnei/plesiomonas shigelloides.	complete assignments for the 1h- and the 13c-nmr spectra of the o-specific polysaccharide of s. sonnei/plesiomonas shigelloides are reported. evidence is presented that in this polysaccharide both pyranose residues exist preferentially in the 4c1 chair conformation and that the polysaccharide exists in the zwitterion form.	1997	9534229
a case of plesiomonas shigelloides cellulitis and bacteraemia from northern europe.	bacteremia caused by plesiomonas shigelloides is a rare event, often associated with consumption of seafood and fresh or estuarine water in temperate or tropical climates. most patients have showed underlying health disorders. here we present a case of p. shigelloides septicaemia and cellulitis of the left hand associated with fish handling in northern sweden (65 degrees latitude north). the patient, who suffered from multiple myeloma, recovered uneventfully after initial treatment with intraven ...	1997	9571748
enteric bacterial pathogens in stools of residents of urban and rural regions in nigeria: a comparison of patients with and without diarrhoea and controls without diarrhoea.	a total of 2,400 stool samples comprising 1,200 from patients with diarrhoea (600 each from urban and rural area) and 1,200 similarly divided controls were obtained from school children and clinic attendants of government and private clinics around three designated study centres of edo, lagos and cross river states, nigeria. these were screened for the prevalence of bacteria that could cause diarrhoea. diarrhoea cases in urban areas had a high prevalence rate for campylobacter spp. (28%), follow ...	1997	9661320
evaluation of the immunospecificity of the porin om1 of vibrio anguillarum serotype o1.	three methods of immunoanalysis (immunoblot, elisa and dot-blot) were used to evaluate the immunospecificity of the antiserum against the porin om1 of vibrio anguillarum serotype o1 with respect to all the serotypes of v. anguillarum, different vibrio species and other gram-negative genera. in the immunoblot analysis of the outer membrane proteins, this antiserum cross-reacted with the main outer membrane protein (momp) of all the vibrio strains studied but not with other genera, except plesiomo ...	1998	9674122
bloody diarrhea caused by plesiomonas shigelloides proctitis in a human immunodeficiency virus-infected patient.		1998	9770179
surveillance for waterborne-disease outbreaks--united states, 1995-1996.	since 1971, cdc and the u.s. environmental protection agency have maintained a collaborative surveillance system for collecting and periodically reporting data that relate to occurrences and causes of waterborne-disease outbreaks (wbdos).	1998	9859954
plesiomonas shigelloides and salmonella serotype hartford infections associated with a contaminated water supply--livingston county, new york, 1996.	on june 24, 1996, the livingston county (new york) department of health (lcdoh) was notified of a cluster of diarrheal illness following a party on june 22, at which approximately 30 persons had become ill. this report summarizes the findings of the investigation, which implicated water contaminated with plesiomonas shigelloides and salmonella serotype hartford as the cause of the outbreak.	1998	9614009
incidence of non-01 vibrio cholerae and aeromonas spp. in fresh water in araraquara, brazil.	the occurrence of aeromonas spp., vibrio cholerae, and plesiomonas shigelloides in fresh water from various sources in araraquara, state of são paulo, brazil was determined. samples from ten distinct irrigation systems used in vegetable cultivation, from five distinct streams, from two reservoirs, from one artificial lake, and from three distinct springs were analyzed. all isolates were serotyped and tested for hemolysin, cytotoxin, heat-stable (st) and heat-labile (lt) enterotoxins production; ...	1998	9625786
congenital endophthalmitis following maternal shellfish ingestion.	to highlight an unusual organism causing a unilateral endophthalmitis by transplacental spread.	1998	9630299
characterization of plesiomonas shigelloides from diarrheic children.	infrastructure of 29 p. shigelloides strains isolated as the only positive finding from children with diarrhea (biochemical properties, antigenic structure, antigenic relationship to shigellae, atb susceptibility and plasmids) were described. a big variety of 22 serovars in a relatively small number of strains was found, inclusive four new 0 (093, 094, 095 and 096) and one new h (h46) antigen. some strains belonged to the so-called "schubert antigenic scheme" the serovars of which come of surfac ...	1998	9524746
[the antimicrobial sensitivity and plasmid isolation in plesiomonas shigelloides].	30 strains of plesiomonas shigelloides isolated from patients with acute diarrheal disease at different health centers of the country were studied. the were phenotypically characterized by conventional biochemical tests and the antimicrobial susceptibility to 11 drugs was determined by the kirby bauer's method. it was found that the strains of plesiomonas shigelloides were sensitive to 7 and resistant to 6 of the investigated drugs. the presence of plasmids in 12 of the 29 analyzed strains was d ...	1998	10349444
free genes for rrnas in the macronuclear genome of the ciliate stylonychia mytilus.	when separated on an agarose gel, macronuclear dna of the hypotrichous ciliate stylonychia mytilus gives rise to many well-defined bands ranging in molecular weight from 0.3 x 10(6) to 14 x 10(6) dalton. hybridization of 25 s rrna, 17s rrna or 5 s rna to such a gel revealed sharp hybridization bands. this suggests that this banding pattern is not an artefact due to nonspecific degradation of macronuclear dna but that the dna in the macronucleus of stylonychia occurs in discrete fragments, each c ...	1998	104846
[the overdiagnosed patient--a legend?].		1998	108562
protocols and "auditable" checklists in ambulatory medical care.		1998	121398
abdominal twiddler's syndrome.		1998	104582
[diagnosis of magnesium deficiency should be encouraged (proceedings)].		1998	108548
prenatal methylmercury poisoning. clinical observations over five years.	thirty-two infants prenatally exposed to methylmercury and their mothers were examined over a five-year period after the iraqi methylmercury epidemic. severity of poisoning in mothers was related to the peak mercury concentration in their hair and in the infants to the maximum concentration in maternal hair during pregnancy. in nine cases of cerebral palsy, methylmercury exposure occurred only during the last trimester. all infants except three (two were orphaned soon after birth and one was bot ...	1999	84530
[evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy].		1999	88669
substrate specificity of hexosaminidase a isolated from the liver of a patient with a rare form (ab variant) of infantile gm2 gangliosidosis and control tissues.		1999	96668
[retinal neovascularization: topological, morphological and hemodynamic features (author's transl)].		1999	91317
activity of aryl hydrocarbon hydroxylase in psoriatic skin.	the activity of aryl hydrocarbon hydroxylase (a.h.h.), a microsomal mono-oxygenase, was reduced in epidermis from both the psoriatic lesions and clinically normal lesion-free skin from psoriatic patients. induction of epidermal a.h.h. activity by benzanthracene was also significantly less than normal in both psoriatic lesions and in clinically normal skin from patients with psoriasis. the enzyme defect may be related to the primary genetic abnormality of the disease.	1999	84948
[incidence of plesiomonas shigelloides in tilapia tetrahibrids (oreochromis sp.)].	plesiomonas shigelloides, a member of the family vibrionaceae, is a gram negative rod associated with several gastroenteritis outbreaks, especially in tropical and subtropical countries. in same way, it has been related to some septicemia, meningitis and cholecystitis cases. the microorganism is normally found in water, fish and birds. the aim of this work was to study the incidence of plesiomonas shigelloides in tetrahybrids of oreochromis sp. (pink tilapia) located at the central region of ven ...	1999	10412509
antimicrobial activity of elaeocarpus sphaericus.	antibacterial activity of petroleum ether (pe), benzene (be), chloroform (ce), acetone (ae) and ethanol (ee) extracts of dried elaeocarpus sphaericus fruit was investigated against 28 gram-positive and gram-negative bacteria using the disc diffusion and plate dilution methods. the ae fraction showed marked antimicrobial activity against ten organisms. be was active against salmonella typhimurium and morganella morganii, and ee against plesiomonas shigelloides, shigella flexnerii and sh. sonneii. ...	1999	10441793
fatal plesiomonas shigelloides in a newborn.		1999	10464413
case-control study of enteropathogens associated with childhood diarrhea in dhaka, bangladesh.	the international centre for diarrhoeal disease research, bangladesh, is a major center for research into diarrheal diseases. the center treats more than 100,000 patients a year. to obtain useful information representative of all patients, a surveillance system in which a 4% systematic sample of all patients is studied in detail, including etiological agents of diarrhea, was installed in october 1979. the first paper on etiology for the surveillance patients was published in 1982, which identifi ...	1999	10523534
cell adhesion of plesiomonas shigelloides.	plesiomonas shigelloides evidences an inconsistent behaviour in the int-407 cell adhesion test. isolates from clinical material evidence regular and pronounced cell adhesion which, however, is low compared with that of clinical aeromonas isolates. in some of the p. shigelloides strains isolated from clinical material, the cell adhesion is markedly more pronounced than in others. the p. shigelloides strains isolated from the natural habitat from both pond water, mud samples and water insects mani ...	1999	10546328
bacterial microflora of wild brown trout (salmo trutta), wild pike (esox lucius), and aquacultured rainbow trout (oncorhynchus mykiss).	initial numbers of bacteria associated with wild (brown trout and pike) and cultured (rainbow trout) freshwater fish as well as with the water in which they were caught were determined. subsequently, a total of 979 randomly selected isolates were characterized and identified to the genus level. for all counts performed (aerobes, psychrotrophs, anaerobes, enterobacteriaceae, and enterococci), no significant differences were observed in water samples, the highest level corresponding to psychrotrop ...	1999	10571316
hemolytic and elastolytic activities influenced by iron in plesiomonas shigelloides.	the aim of this study was to determine the presence of hemolytic and elastolytic enzymes in several strains of plesiomonas shigelloides in relation to the availability of iron in culture media. hemolytic activity and elastolytic activity were detected in strains of p. shigelloides and were enhanced when the strains were grown in an iron-depleted medium and lost after thermal treatment at 100 degrees c for 10 min. also, elastolytic activity was inactivated by phenylmethyl sulfonyl fluoride, an in ...	1999	10606155
plesiomonas shigelloides in water and fish.	plesiomonas shigelloides strains isolated from aquariums, surface water and fish were serotyped using the international antigenic scheme and a provisional scheme designed for environmental strains, called the "schubert" scheme. a remarkable variety of serovars represented in both schemes was found, namely in surface water samples and in fish.	1999	10659376
plesiomonas shigelloides and its serovars in animals in the czech republic--region moravia.	in the period of 1994-1997, 4,552 samples of sectional, clinical material and environment were examined for the presence of plesiomonas shigelloides. in 55 cases (1,21%) plesiomonas shigelloides was isolated. within 55 isolated strains, 26 serovars have been successfully identified. among other secured cases was plesiomonas shigelloides isolated in pig (sus scrofa domestica), mouse mus musculus), binturong (arctictis binturong) and raccoon (pocyon lotor). in mandarin duck (aix galericulata) the ...	1999	10084023
[bacteriological studies of traveller's diarrhoea (6). analysis of enteropathogenic bacteria at kansai airport quarantine station from september 4th, 1994 through december 1996].	during the period of investigation from sept. 4, 1994 to dec, 1996, a total of 11,446,534 overseas travellers were quarantined at kansai airport quarantine station, and 22,187 voluntarily reported of episodes suffering from diarrhoea. bacteriological examination of the stools a total of 9,299 individuals' was performed, and the following results were obtained. 1) various enteropathogenic bacteria were isolated from 33.3% of the stools examined. bacterial species isolated were as follows: plesiom ...	1999	10213987
isolation, biochemical and serological characterisation of plesiomonas shigelloides from freshwater in northern europe.	isolation and characterisation of plesiomonas shigelloides from fresh water in northern europe is reported in this study. the organisms were isolated from two lakes and a river in sweden. all isolates of p. shigelloides showed an identical biochemical profile and belonged to different serotypes, namely, o18, o23, o26, o58 and o60. the study indicates that p. shigelloides may occur in the aquatic environment of cold climates and as a result, it is likely to be associated with human infections cau ...	2000	10660257
structural studies of the o-specific polysaccharide from plesiomonas shigelloides strain cnctc 113/92.	the structure of the o-specific side chain of the lipopolysaccharide (lps) of plesiomonas shigelloides, strain cnctc 113/92 has been investigated by nmr spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. it was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which d-beta-d-hepp is dglycero-beta-dmanno-heptopyranose and 6d-beta-d-hep is 6-deoxy-beta-dmanno- ...	2000	10712598
plesiomonas shigelloides--an uncommon cause of diarrhoea in cats?		2000	10749048
the complete dna sequence of the o antigen gene region of plesiomonas shigelloides serotype o17 which is identical to shigella sonnei form i antigen.	we cloned and determined the sequence of a dna region of approximately 15-kb containing the cluster of genes required for o17 antigen expression in the escherichia coli k-12 strain from the chromosome of plesiomonas shigelloides serotype o17:h2 strain. the sequencing analysis revealed that the minimum essential region of the p. shigelloides o17 antigen gene cluster had a size of approximately 11.5-kb and contained 9 contiguous open reading frames (orfs), which were almost identical to the corres ...	2000	10789503
plesiomonas shigelloides--an uncommon cause of diarrhoea in cats?		2000	10791473
beta-lactamase expression in plesiomonas shigelloides.	we have examined the production of beta-lactamases by 11 clinical and nine environmental isolates of plesiomonas shigelloides from czechoslovakia, the czech republic and cuba. ten isolates (50%) expressed detectable amounts of a chromosomally encoded, non-inducible beta-lactamase, though all isolates showed a broadly similar resistance profile: low-level resistance to ampicillin and higher-level resistance to carbenicillin. all strains were susceptible to cephalosporins and meropenem. three clin ...	2000	10837443
31p-nmr and 13c-nmr studies of mannose metabolism in plesiomonas shigelloides. toxic effect of mannose on growth.	the metabolism of mannose was examined in resting cells in vivo using 13c-nmr and 31p-nmr spectroscopy, in cell-free extracts in vitro using 31p-nmr spectroscopy, and by enzyme assays. plesiomonas shigelloides was shown to transport mannose by a phosphoenolpyruvate-dependent phosphotransferase system producing mannose 6-phosphate. however, a toxic effect was observed when p. shigelloides was grown in the presence of mannose. investigation of mannose metabolism using in vivo 13c nmr showed mannos ...	2000	10931197
prevalence and virulence properties of vibrio cholerae non-o1, aeromonas spp. and plesiomonas shigelloides isolated from cambé stream (state of paraná, brazil).	the incidence of vibrio cholerae, aeromonas spp. and plesiomonas shigelloides was determined in water samples from cambé stream. the samples were collected from seven different sites. the serogroups, virulence markers and drug resistance profiles were also evaluated. twelve aer. hydrophila, 12aer. caviae, eight aer. sobria, seven ple. shigelloides and two v. cholerae non-o1 were isolated. they belonged to different serogroups and all produced haemolysis in different assays. five of the aeromonas ...	2000	10945781
new serovars of plesiomonas shigelloides--1992-1998.	most of the 26 new o (o77-o102) and 10 new h (h42-h50) and h1a1d antigens were found in various p. shigelloides strains isolated from man and other mammals, birds, fish, and water and water insects, not only in the czech republic but also in 12 foreign countries.	2000	10965437
reduction of endogenous bacteria associated with catfish fillets using the grovac process.	fresh catfish (ictalurus punctatus) fillets are known to be contaminated with a large number of spoilage and pathogenic bacteria. the grovac method, a new patented (u.s. 5,543,163) process, was evaluated for its efficacy in reducing the number of pathogens and spoilage microorganisms associated with food. this process involves using a processing solution containing ascorbic acid (aa) and sodium chloride (nacl), vacuum, and tumbling. a total of 51 bacterial isolates were isolated and identified f ...	2000	10983798
comparison of o-antigen gene clusters of escherichia coli (shigella) sonnei and plesiomonas shigelloides o17: sonnei gained its current plasmid-borne o-antigen genes from p. shigelloides in a recent event.	escherichia coli sonnei has an o antigen identical to that of plesiomonas shigelloides o17, and its o-antigen gene cluster is located on a plasmid. by sequencing the chromosomal o-antigen gene cluster of p. shigelloides o17 and comparing it with that of sonnei, we showed that sonnei gained its o-antigen genes recently.	2000	10992522
specific detection of plesiomonas shigelloides isolated from aquatic environments, animals and human diarrhoeal cases by pcr based on 23s rrna gene.	twenty-five strains of plesiomonas shigelloides isolated from aquatic environment, 10 strains from human cases of diarrhoea and five strains from animals were identified by the polymerase chain reaction technique based on 23s rrna gene. for this purpose, two primers targeted against part of the 5' half of the 23s rrna gene of p. shigelloides (escherichia coli number c-912, g-1195; plesiomonas number c-906, g-1189) were designed. results from our study indicated that this method might serve as a ...	2000	11024349
[plesiomonas shigelloides, a vibrionaceae to be taken into account].	the antigenic structure and antimicrobial susceptibility were studied in 99 strains isolated from patients with acute diarrhea (6 strains from an outbreak of digestive transmission disease in santiago de cuba) and a strain isolated from a patient who died from infections neurological syndrome (ins, meningitis). four new serotypes (093, 994, 095, 096), which had not been described in the world classification, were identified from the cuban isolated strains and were included in the international s ...	2000	11107887
plesiomonas shigelloides infection in hong kong: retrospective study of 167 laboratory-confirmed cases.	to study the epidemiological, clinical, and microbiological features of plesiomonas shigelloides infection in hong kong.	2000	11177159
an epidemiological study of plesiomonas shigelloides diarrhoea among japanese travellers.	plesiomonas shigelloides is often regarded as a non-pathogenic bacterial species that is occasionally isolated from patients with diarrhoea. however, a review of travellers returning to japan with diarrhoeal illness through kansai airport revealed that the incidence of p. shigelloides from microbiologically confirmed cases increased from 23.2% in 1987 to 77.8% in 1999. we carried out a descriptive epidemiological study to identify patterns associated with diarrhoea due to this organism. selected ...	2000	11218202
new o and h antigens of the international antigenic scheme for plesiomonas shigelloides.	a revised update of the international antigenic scheme for plesiomonas shigelloides is presented. twenty-six new o (o77-o102) and 10 new h (h42-h50 and h1a1d) antigens have been described since 1994. the sources of antigens are mostly human clinical strains, isolates from warmblooded animals and a few environmental cultures.	2000	11347249
improvement in iron status and liver function in patients with transfusional iron overload with long-term subcutaneous desferrioxamine.	subcutaneous desferrioxamine (2--4 g over 12 h) was administered 6 nights each week to 34 patients with transfusional iron overloads who continued to receive regular blood-transfusions. all 34 patients showed a fall in serum-ferritin after 5 to 12 months. in some patients serum-ferritin fell almost to normal. liver function improved in all the patients, serum-aspartate-transaminase levels fell in all 17 patients tested, and liver-iron fell in 5 of 6 patients tested. these studies show that body- ...	2000	87616
selective modification of the renal and the hepatic toxicities of chloroform by induction of drug-metabolizing enzyme systems in kidney and liver.	renal and hepatic microsomal enzyme activities were measured in icr male mice treated with phenobarbital (pb), 3-methylcholanthrene (3mc), 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) or polychlorinated biphenyls (pcb). the effect of these agents on subsequently administered single doses of chloroform (chci3) were also determined. 3mc, tcdd and pcb increased renal as well as hepatic microsomal enzyme activities. pb increased hepatic but not renal microsomal enzyme activities. the hepatotoxicity of ...	2000	101655
the medical treatment of epilepsy: antiepileptic drug interactions and teratogenicity.		2000	88711
[improvement of blood viscosity after infusion of low-molecular hydroxyethyl starch (expafusin) in healthy subjects].	in healthy volunteers, 500 ml of a 6% low molecular weight hydroxyethyl starch solution (expafusin) was infused intravenously within 30 minutes. blood samples for the measurement of rheological parameters were obtained before and after the infusion. blood and plasma viscosity were significantly decreased after the infusion of hes. red cell deformability as measured by a filtration technique was slightly improved. red cell aggregation as measured by a light transmission method was markedly reduce ...	2000	94316
deafness due to hypervitaminosis d.		2000	87663
two-week postnatal examination of infants.		2000	87641
steroids in asthma.		2000	91910
[cryptorchism: hormonal treatment].		2000	27065
review of laxative utilization in a skilled nursing facility.	laxatives are the most frequently prescribed drugs in long-term care facilities. of all nursing home patients, 58 percent receive laxatives--often more than one, and usually on a "prn" basis. this pattern of dosage is probably used in an effort to prevent constipation. selection of a laxative should be based on careful evaluation of the cause of constipation, with due consideration being given to differences in drug effectiveness and the incidence of side effects. a review of laxative utilizatio ...	2000	101574
runaway cordis omni-stanicor pacemaker.		2001	95306
psychiatry - questions but no answers.		2001	98154
the hook sign in type 3 congenital colonic atresia.		2001	98027
[questions to television from psychopathology].	progress is expected in psychopathological diagnosis by using audiovisual procedures. nowadays, a valid psychopathology concept should be broadly planned and the important method of description as well as understanding procedures should be considered. for such a model of psychopathology it seems appropriate to investigate the different psychopathological phenomenons as expression of a disturbed communication ability. concerning this outlook, new strategies are to be developed or procedures of ot ...	2001	90381
indian childhood cirrhosis.		2001	90306
chinese hamster purine nucleoside phosphorylase.		2001	99640
nelson's eye.		2001	87735
primary prevention oregon style.		2001	98505
perinatal mortality.		2001	93220
morphological variation in oat-cell carcinoma of the bronchus.		2001	87879
ddavp and cryoprecipitate in mild haemophilia.		2001	90263
rrn operons in haemophilus parainfluenzae and mosaicism of conserved and species-specific sequences in the 16s-23s rdna long spacer.	the mosaic organisation of short-sequence boxes was analysed in the cloned and sequenced long ribosomal spacer (547 bp) of haemophilus parainfluenzae gr. comparison and alignment of both the long and the short spacer were performed in h. parainfluenzae and h. influenzae rd. the long spacer contained two trna genes (trna(ala) and trna(ile)) which are highly homologous to the corresponding genes found in the spacers of other species, such as haemophilus spp., actinobacillus spp., and plesiomonas s ...	2001	11446514
bacterial causes of aids-associated diarrhea in thailand.	the incidence of bacterial diarrhea in aids patients has increased steadily and has led to enormous medical and public health problems. in this study, the clinical data together with 350 rectal swab samples each from aids patients with diarrhea (apd) and non-aids patients with diarrhea (napd), were collected and examined for bacterial enteropathogens at the bamrasnaradura infectious diseases hospital (bidh), nonthaburi, thailand from may to december 1996. patients were matched by age and sex. th ...	2001	11485080
beta-lactam-susceptibility patterns of plesiomonas shigelloides strains: importance of inoculum and medium.	the susceptibility of 10 plesiomonas shigelloides strains to 30 beta-lactam antibiotics was examined. susceptibility testing was carried out with a microdilution procedure using 3 media (isosensitest broth, mueller-hinton broth and cation-adjusted mueller-hinton broth) and 4 inocula [1 x 10(4), 1 x 10(5), 1 x 10(6) and 1 x 10(7) colony-forming units (cfu)/ml]. a high inoculum dependency of mic values was found for numerous beta-lactams. each strain showed 2 completely different susceptibility pa ...	2001	11669228
[surveillance of various enteropathogenic bacteria from diarrheal cases during 1989-1999 in kobe city].	incidence of various enteropathogenic bacteria was examined from diarrheal faecal samples that were collected from the patients of kobe city general hospital and some station hospitals (23,862), and from overseas travelers (2,855) over a period of decade (1989-1999) in kobe. a total of 1,580 strains were isolated from domestic and 331 strains from overseas travelers. the results are as follows. 1) thirteen kinds of enteropathogenic bacteria were isolated from domestic diarrheal cases (6.6%). sal ...	2001	11712364
natural antimicrobial susceptibilities of plesiomonas shigelloides strains.	the natural susceptibility of 74 plesiomonas shigelloides strains isolated from humans (n = 50), water (n = 22) and animals (n = 2) to 71 antibiotics was examined. mics were performed using a microdilution procedure with mueller-hinton broth and an inoculum of 1 x 10(6) cfu/ml. plesiomonas strains were naturally susceptible or naturally susceptible and intermediate to tetracyclines, several aminoglycosides, aminopenicillins in combination with beta-lactamase inhibitors, all cephalosporins except ...	2001	11733464
plesiomonas shigelloides sepsis and splenic abscess in an adolescent with sickle-cell disease.	plesiomonas shigelloides is a rare cause of self-limiting gastroenteritis. we report a case of extraintestinal p. shigelloides infection in an adolescent with sickle-cell disease who presented with bacteremia complicated by a splenic abscess. despite the high mortality rate reported in extraintestinal p. shigelloides infection, the patient survived after drainage of the abscess and treatment with antibiotics.	2001	11740330
[gastroenteritis due to plesiomonas shigelloides--rare cases in the western world].	plesiomonas shigelloides is a common pathogen in tropical regions, whereas it is rarely isolated in temperate climates. it is most often found in surface water and fish. during the last 10 years it was found to cause gastroenteritis 6 times in ludwigshafen. not all of these patients reported a trip to foreign countries.	2001	11253281
plesiomonas shigelloides enters polarized human intestinal caco-2 cells in an in vitro model system.	this study provides the first definitive evidence that the gram-negative bacterium plesiomonas shigelloides adheres to and enters eukaryotic intestinal host cells in vitro. p. shigelloides is increasingly regarded as an emerging enteric pathogen and has been implicated in intestinal and extraintestinal infections in humans. however, the establishment of its true role in enteric disease has been hindered by inadequacies in experimental design, deficiencies in clinical diagnosis, and the lack of a ...	2001	11254582
characterization of the plesiomonas shigelloides genes encoding the heme iron utilization system.	plesiomonas shigelloides is a gram-negative pathogen which can utilize heme as an iron source. in previous work, p. shigelloides genes which permitted heme iron utilization in a laboratory strain of escherichia coli were isolated. in the present study, the cloned p. shigelloides sequences were found to encode ten potential heme utilization proteins: huga, the putative heme receptor; tonb and exbbd; hugb, the putative periplasmic binding protein; hugcd, the putative inner membrane permease; and t ...	2001	11292789
effects of medium composition, calcium, iron and oxygen on haemolysin production by plesiomonas shigelloides isolated from water.	the effects of medium composition, calcium, iron and oxygen tension on the haemolytic activity of plesiomonas shigelloides were investigated.	2001	11298246
pub6060: a broad-host-range, dna polymerase-i-independent cole2-like plasmid.	a cole2-like, cryptic plasmid, pub6060, of 5.8 kb has been found in a clinical isolate of plesiomonas shigelloides. the complete sequence of pub6060 has been determined and reveals a number of interesting features about the plasmid. the cole2-like replication locus is linked to a functional cole1-like mobilization locus. replication is, unusually for cole2 replicons, dna polymerase-i-independent and may involve two, rather than the usual one, plasmid-encoded functions. additionally, it carries t ...	2001	11322823
identification and functional characterization of arylamine n-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid.	arylamine n-acetyltransferase activity has been described in various bacterial species. bacterial n-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. we characterized these enzymes further by steady-state kinetics, time-dependent inhibition, and dna hybridization in 40 species, mostly from the human intestinal microflora. we report for the first time n-acetyltransferase activity in 11 ...	2001	11344150
enteropathogenicity of plesiomonas shigelloides and aeromonas spp. in experimental mono- and coinfection with cryptosporidium parvum in the intestine of neonatal balb/c mice.	enteropathogenicity of plesiomonas shigelloides, aeromonas hydrophila, a. caviae and a. sobria was studied both in monoinfections and in coinfections with coccidium cryptosporidium parvum in neonatal balb/c mice. in monoinfection experiments, neonatal balb/c mice were orally infected with 7 x 10(7) or 7 x 10(8) cfu, respectively, of a strain of p. shigelloides or a strain of an aeromonas spp. in coinfection experiments, the neonatal mice were, in addition to being orally infected with one of the ...	2001	11131040
case report. epididymo-orchitis and bacteremia caused by plesiomonas shigelloides in an hiv-infected patient.	invasive infection and extraintestinal complications are rarely caused by plesiomonas shigelloides, a waterborne bacterium implicated in gastroenteritis. recent evidence from several reports suggests its importance in causing opportunistic infections in immunocompromised hosts. we report a case of p shigelloides bacteremia and epididymo-orchitis in a patient with hiv infection and chronic hepatitis c. this case illustrates a novel site of involvement by p shigelloides and underscores its role as ...	2001	11806175
isolation and preliminary characterization of bacteriocins produced by vibrio vulnificus.	the aim of this work was to isolate bacteriocins from the environment that would be effective in neutralizing vibrio vulnificus in seafood.	2002	11849361
characteristics of plesiomonas shigelloides infection in taiwan.	from january 1999 through december 2000, a total of 111 cases of plesiomonas shigelloides infections were diagnosed in a medical center of northern taiwan. the patients ranged in age from 22 days to 72 years. one third (39/111, 35%) of the positive cultures were found in young children (<2 years old). there was no significant difference in the incidence between males (56/111, 50%) and females (55/111, 50%). the peak seasons for the disease were summer (45/111, 41%) and autumn (42/111, 38%). the ...	2002	11950120
[atypical presentation of plesiomonas shigelloides infection].		2002	12006265
[isolation of plesiomonas shigelloides from a pediatric patient].	we report the isolation of p. shigelloides in the stools of a child with acute diarrhea living in the surroundings of buenos aires who usually drank untreated water. p. shigelloides has been implicated as an etiological agent in many sporadic cases of diarrhea, probably associated with drinking untreated water, eating uncooked seafood or foreign travel.	2002	12180255
[severe choleriform diarrhea from plesiomonas shigelloides in an expatriate from djibouti].		2002	12192722
plesiomonas shigelloides sepsis in a thalassemia intermedia patient.	bacteremia due to plesiomonas shigelloides was associated with rapidly fulminant septicemia, disseminated intravascular coagulation and massive adrenal hemorrhage in a splenectomized patient suffering from thalassemia intermedia who was treated with hydroxyurea. p. shigelloides was isolated in blood cultures; despite a vigorous combination of antibiotics the patient died after 24 h in the icu. lethal sepsis due to p. shigelloides has not previously been reported in greece.	2002	12374363
donor substrate regeneration for efficient synthesis of globotetraose and isoglobotetraose.	here we describe the efficient synthesis of two oligosaccharide moieties of human glycosphingolipids, globotetraose (galnacbeta1-->3galalpha1-->4galbeta1-->4glc) and isoglobotetraose (galnacbeta1-->3galalpha1-->3galbeta1-->4glc), with in situ enzymatic regeneration of udp-n-acetylgalactosamine (udp-galnac). we demonstrate that the recombinant beta-1,3-n-acetylgalactosaminyltransferase from haemophilus influenzae strain rd can transfer n-acetylgalactosamine to a wide range of acceptor substrates ...	2002	12406759
diarrhoeagenic bacterial pathogens in hiv-positive patients with diarrhoea in rural communities of limpopo province, south africa.	potential enteric bacterial pathogens in 60 hiv-positive patients with chronic diarrhoea in rural communities of the limpopo province, south africa, were identified using standard microbiological methods. the kirby-bauer disk-diffusion method was employed to determine antibiograms of isolated bacteria. results revealed that diarrhoeagenic bacterial agents were isolated from 48 (80%) of the 60 hiv-positive patients with diarrhoea. forty-four (73.3%) and 16 (26.7%) of the 60 patients were female a ...	2002	12430759
new udp-glcnac c4 epimerase involved in the biosynthesis of 2-acetamino-2-deoxy-l-altruronic acid in the o-antigen repeating units of plesiomonas shigelloides o17.	plesiomonas shigelloides is a ubiquitous waterborne pathogen responsible for diseases such as diarrhea and bacillary dysentery, commonly afflicting infants and children. this bacterium is endowed with an o-antigen gene cluster consisting of 10 consecutive reading frames. one of these, designated wbgu (orf3), has been overexpressed and biochemically characterized to show that it encodes a uridine diphosphate-n-acetylglucosamine (udp-glcnac) c4 epimerase, only the second microbial enzyme character ...	2002	12484781
phylogenetic study and identification of human pathogenic vibrio species based on partial hsp60 gene sequences.	the use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. a 600-bp partial hsp60 gene was amplified by pcr and sequenced from 29 strains representing 15 vibrio species within the family vibrionaceae. sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different vibrio species and 96-100% sequence identity among epidemiologically distinct strains with the same species designation. this degre ...	2002	12489780
pyosalpinx: not always a sexual transmitted disease? pyosalpinx caused by plesiomonas shigelloides in an immunocompetent host.	plesiomonas shigelloides are ubiquitous gram-negative bacteria that are found in fresh or marine water, particularly in tropical or warm climates; they were recently implicated in diarrhoeal disease. patients usually present with a history of recent travel to tropical regions or consumption of uncooked seafood. extraintestinal disease has rarely been reported, occurring generally in neonates or immunocompromised patients, and is often fatal. we report a case of right pyosalpinx due to p. shigell ...	2002	12519354
core oligosaccharides of plesiomonas shigelloides o54:h2 (strain cnctc 113/92): structural and serological analysis of the lipopolysaccharide core region, the o-antigen biological repeating unit, and the linkage between them.	the structure of the core oligosaccharide moiety of the lipopolysaccharide (lps) of plesiomonas shigelloides o54 (strain cnctc 113/92) has been investigated by (1)h and (13)c nmr, fast atom bombardment mass spectrometry (ms)/ms, matrix-assisted laser-desorption/ionization time-of-flight ms, monosaccharide and methylation analysis, and immunological methods. it was concluded that the main core oligosaccharide of this strain is composed of a decasaccharide with the following structure: (see text) ...	2002	11796731
the surgical management of low back and sciatic syndrome in disc disease or injury : results of a joint neurosurgical and orthopaedic project.		2002	127575
[instrumental perforations of the esophagus].		2002	139654
paracoccidioidal infection in the wife of a patient with paracoccidioidomycosis.	three agar immunoprecipitin techniques (double immunodiffusion, immunoelectrophoresis and immunoelectroosmophoresis-immunodiffusion) made with paracoccidioidin and serum of a paracoccidioidomycosis patient's wife, permitted us to observe a specific band in 6 successive samples of blood taken from the wife over a period of 21 months. the case represents an example of a subclinical paracoccidioidomycosis infection that is usually diagnosed in its disseminated, progressive form. three possible sour ...	2002	120614