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purification and characterization of an extracellular cytolysin produced by vibrio vulnificus.an extracellular cytolytic toxin produced by the halophilic bacterium vibrio vulnificus was isolated free of detectable contamination with medium constituents and other bacterial products by sequential ammonium sulfate precipitation, gel filtration with sephadex g-75, hydrophobic interaction chromatography with phenyl-sepharose cl-4b, and isoelectric focusing in an ethylene glycol density gradient. the cytolysin is a heat-labile, hydrophobic protein that is inhibited by large amounts of choleste ...19853980095
metastatic nontraumatic clostridium septicum osteomyelitis.nontraumatic clostridial infections are rare, but need to be diagnosed and treated early or high morbidity and mortality rates result. we believe that this is the first reported case of metastatic nontraumatic clostridium septicum osteomyelitis. early treatment with surgical debridement and parenteral antibiotics without hyperbaric oxygen was used. an associated occult rectal malignancy was discovered and treated.19853980945
molecular mechanisms of olfactory reception. vi. kinetic characteristics of camphor interaction with binding sites of rat olfactory epithelium.camphor binding to a possible receptor of rat olfactory epithelium has been studied within the ligand concentration range 10(-11)-10(-6) m. at these concentrations camphor is bound by a set of receptors. they are distinguished by both the affinity to the ligand (k1 = 5 x 10(-10) m, k2 = 3.5 x 10(-8) m, k3 approximately equal to 10(-6) m) and their amount in the epithelium. the differences in the affinities are due to different values of the association rate constant of camphor (k1), which varies ...19853986222
vibrio vulnificus. man and the sea.to identify risk factors for vibrio vulnificus infections, we performed a regional case-control study of 19 patients identified by isolates received at a state reference laboratory. interviews with patients or surviving relatives and with three controls for each patient were compared in a matched analysis. patients with v vulnificus wound infection were more likely than controls to have sustained a puncture wound while handling fresh seafood or to have been exposed to salt water. more patients w ...19853989959
[persistent chlamydia infection in cell culture]. 19853993192
vibrio vulnificus and patients with liver disease. 19853996803
fatal septicaemia due to vibrio vulnificus. 19854002991
surgical treatment for infective endocarditis--decision tree for the treatment and introduction of translocation method.persistent fever during active-phase ie is an indication for surgical treatment in the light of the causative organism and the underlying disease. to counter worsening hemodynamics, surgery may be the only feasible way. surgery is performed when the patient does not respond to vasodilators and he shows an fs of less than 25%. the introduction of translocation provides a solution for the treatment of active ie. lesions, vegetations more than 5 mm in diameter, periannular abscess, and mycotic aneu ...19854021068
topographic organization of projections from the amygdala to the hypothalamus of the rat.afferent fibers from the amygdala to subdivisions of lateral, ventromedial and dorsomedial hypothalamic nuclei were investigated in rat by retrograde transport of horseradish peroxidase. small (intranuclear size) peroxidase deposits were placed in hypothalamic nuclei by iontophoresis of a tracer solution containing poly-l-alpha-ornithine which greatly limited diffusion. the medial, central and amygdalo-hippocampal nuclei of the amygdala were found to be the major donors of amygdaloid afferent fi ...19854022458
detection of reticulo-endothelial blockade with low-dose test agent.blood clearance and organ extraction of a low-dose reticulo-endothelial test agent, technetium labelled tin colloid (ttc), was measured in groups of rabbits pretreated with reticulo-endothelial blocking agents. electron microscopy and ultrastructure analysis confirmed that kupffer cells extracted ttc. pretreatment with silica caused reduced kupffer cell uptake and spillover of ttc into the spleen. pretreatment with sheep red cells caused reduced kupffer cell uptake and reduced splenic uptake but ...19854027180
vibrio vulnificus resists phagocytosis in the absence of serum opsonins.invasive disease caused by vibrio vulnificus may result partially from resistance to phagocytic host defense mechanisms. the present studies show that v. vulnificus resists phagocytosis by murine peritoneal macrophages in the absence of serum opsonins and extracellular bacterial products, apparently through the anti-phagocytic properties of the bacterial surface.19854030101
the incidence of rhodococcus (corynebacterium) equi in domestic animals and soil. 19854032938
the early revascularization of membranous bone.the experimental finding that membranous onlay bone grafts maintain volume and viability to a greater extent than do endochondral grafts may be related to the more rapid vascularization of membranous bone. microangiographic techniques were used to study the rates of vascularization of membranous and endochondral bone grafts in adult white new zealand rabbits at 1, 3, 7, 14, and 21 days after bone grafting. vascularization patterns were quantified microscopically using a modified point-counting t ...19854034769
vibrio vulnificus septicemia.a 33-year-old japanese male, who had a three year history of biopsy-proved liver cirrhosis, was admitted to the hospital on june, 24, 1983 with a sudden onset of fever (38.6 degrees c), chills, generalized pain, nausea, anorexia, weakness, and eruption over the entire body. the patient went into shock and died about 7 hours after admission. blood cultures before death were positive for v. vulnificus. postmortem microscopic examination revealed "necrotizing vasculitis" in the small and large inte ...19854036604
the effects of hypolipidaemic benzodiazepines on serum lipoproteins in hyperlipidaemic rats. 19854037206
vibrio vulnificus in sea water: a possible cause of acute diarrhea in man? 19854038051
inhibition of lipases by proteins. a kinetic study with dicaprin monolayers.we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ...19854038705
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...19854040921
aryl hydrocarbon hydroxylase induction in adult rat hepatocytes in primary culture by several chlorinated aromatic hydrocarbons including 2,3,7,8-tetrachlorodibenzo-p-dioxin.the induction of aryl hydrocarbon hydroxylase (ahh) by aroclor 1254, 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) and other polychlorinated organic compounds was examined in primary cultures of adult rat hepatocytes isolated by a collagenase perfusion technique. following exposure of fresh hepatocyte cultures to 1 microgram/ml aroclor 1254, ahh induction was undetected for 48 hr and then increased dramatically up to 96 hr. cultures maintained in control medium for either 24, 48, or 72 hr prior to ...19854043593
treatment of local complications of bcg vaccination. 19854049527
antiarrhythmic and electrophysiologic actions of clofilium in experimental canine models.clofilium was studied in three experimental models. in non-ischemic and chronically infarcted canine hearts, clofilium (0.5-2 mg/kg) produced a dose-dependent increase in electrical ventricular fibrillation threshold (vft), but prolonged the effective refractory period (erp) of normal myocardium in only the non-ischemic heart. when chronically infarcted hearts were subjected to programmed electrical stimulation, 1 mg/kg of clofilium inhibited the re-induction of either ventricular tachycardia or ...19854054216
characterization of a mitochondrial transport system for branched chain alpha-keto acids.efflux of branched chain alpha-keto acids from preloaded rat heart mitochondria was slow at low external ph. efflux was first order, and measured rate constants, kappa efflux, were 0.104 +/- 0.005 and 0.115 +/- 0.006 min-1 for alpha-ketoisovalerate and alpha-ketoisocaproate (kic), respectively. efflux was stimulated significantly by branched chain alpha-keto acids and related carboxylates such as alpha-ketocaproate and alpha-ketovalerate, but not by substrates for the pyruvate transporter. kic w ...19854055776
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...19854056003
[scintillation assay for evaluating chemosensitivities of human tumors--the basic principle and clinical utility]. 19854056552
cloning of the cytotoxin-hemolysin gene of vibrio vulnificus.genes encoding the cytotoxin-hemolysin of vibrio vulnificus were cloned in escherichia coli by using the lytic cloning vector, lambda 1059. subcloning in plasmid pbr325 resulted in the isolation of a 3.2-kilobase dna fragment containing the cytotoxin gene. by using this fragment as a dna probe, homologous gene sequences were detected in all 54 v. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.19854066036
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 50-1985. a 41-year-old man with diffuse tracheal narrowing. 19854069162
some properties of vibrio vulnificus hemolysin.some properties of hemolysin produced by vibrio vulnificus were investigated. the hemolysin was heat labile, and the hemolytic activity was inhibited by adding cholesterol or divalent cations. cholesterol inhibited the temperature-independent hemolysin-binding step, suggesting that cholesterol made up the binding site of the cell membrane, whereas the divalent cations inhibited the temperature-dependent membrane-degradation step. however, the v. vulnificus hemolysin was stable to oxygen and sulf ...19854088099
uptake and clearance of vibrio vulnificus from gulf coast oysters (crassostrea virginica).oysters collected in late winter, when they were free of vibrio vulnificus, were exposed in the organism in the laboratory. the oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. these results suggest that v. vulnificus may be found in oysters as a result of filtration of the bacteria from seawater rather than active multiplication of the bacteria in the oysters.19854091570
[an experimental study on adhesion blocking in simultaneous injury of tendon and nerve].the formation of adhesions between simultaneously injured tendon and nerve with and without adhesion blocking materials was studied by using a rabbit model. a collagen membrane, produced from adult bovine corium and with a negative electric charge induced by chemical modification, was used as the blocking material. microangiography and histological studies were performed to investigate the vascular supply to the adhesions. the following results were obtained. blood vessel proliferation was obser ...19853831164
[effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. 19853835674
isolation and characterization of neuropeptide y from porcine intestine.the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin.19853838090
chemoattractants in fibrotic disorders.fibrosis represents an excessive deposition of connective tissue which impedes the normal functions of an organ or tissue. the mechanisms leading to this increased deposition of connective tissue may be similar to those occurring in normal wound repair. we have previously shown that the repair process involves the migration of connective tissue cells to the site of injury and their subsequent proliferation. one of the principal factors controlling these events appears to be the platelet-derived ...19853851719
human c-myc and n-ras expression during induction of hl-60 cellular differentiation.the genome of the human hl-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming n-ras oncogene. the present study has monitored c-myc and n-ras expression in hl-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-o-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin d3. the results demonstrate that induction of hl-60 differentiation is associated with decreases in c-myc rna, while ther ...19853856429
selective uptake of a toxic lipophilic anthracycline derivative by the low-density lipoprotein receptor pathway in cultured fibroblasts.n-(n-retinoyl)-l-leucyldoxorubicin 14-linoleate (r11-dox), a new lipophilic derivative of doxorubicin, was synthesized and incorporated into low-density lipoprotein (ldl). the drug-ldl complex contained 100-200 drug molecules/ldl particle. when cultured normal human fibroblasts were incubated with 125i-ldl-incorporated drug, there was a perfect correlation between the cellular uptake plus degradation of 125i-ldl and the cellular drug accumulation. the presence of excess native ldl inhibited the ...19853856679
in vitro activity of ci-934, a quinolone carboxylic acid active against gram-positive and -negative bacteria.ci-934 is a totally synthetic difluorinated quinolinecarboxylic acid with an ethyl-amino-methyl pyrrolidine side chain, which has broad-spectrum antibacterial activity, including particular potency directed against streptococci and staphylococci. the ci-934 mic (micrograms per milliliter) for 90% of the strains tested was 0.4 (range, 0.2 to 0.8) for a group of streptococci (pneumococci, viridans streptococci, streptococcus faecalis, and lancefield groups a, b, and c), 0.2 (0.05 to 0.2) for staph ...19853866513
characterization with monoclonal antibodies of a surface antigen of plasmodium falciparum merozoites.the merozoite, the extracellular form of the erythrocyte stage of the malarial parasite, invades the erythrocyte and develops intracellularly. cloned hybridoma cell lines secreting monoclonal antibodies directed against the merozoite surface were selected by indirect immunofluorescent assay by using intact isolated merozoites. monoclonal antibodies to a 200,000 m.w. merozoite surface antigen were selected and were used to characterize this protein and its role in erythrocyte invasion. immunoelec ...19853881529
cloning and expression in escherichia coli of vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes.two hemolysin genes of vibrio parahaemolyticus wp1, a thermostable direct (tsd) hemolysin gene and a thermolabile hemolysin gene, were cloned into the pbr322 vector in escherichia coli k-12 c600. a large amount of the tsd hemolysin produced in e. coli k-12 accumulated in the periplasmic space. the tsd hemolysin gene was localized on a 0.9-kilobase hindiii-bamhi fragment by identifying qualitatively the production of the tsd hemolysin by a reverse passive hemagglutination assay in the osmotic sho ...19853886626
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...19853890260
cardioprotective effects of defibrotide in acute myocardial ischemia in the cat.we investigated the effects of defibrotide (d), a natural polydeoxyribonucleotide, on acute myocardial ischemia (ami) in anesthetized cats. a permanent ligature was placed around the left anterior descending coronary artery (lad) 12-14 mm from its origin. ventricular fibrillation and death were exceptional and when they occurred the cats were not included in the evaluation. pretreatment of cats with d, 32 mg kg-1 h-1, i.v. infusion, maintained throughout the 5 h occlusion period, reduced ami-st ...19853890260
spontaneous vibrio vulnificus peritonitis and primary sepsis in two patients with alcoholic cirrhosis.two patients with alcoholic cirrhosis were seen on two separate occasions for fever, swollen legs, petechial hemorrhage, purpura, and cutaneous bullae. one patient ate oysters 2 days before the onset of illness. vibrio vulnificus, a lactose-positive halophilic vibrio, was isolated from the ascitic and cutaneous fluid in both cases, and from the blood in one of the two cases. both isolated strains were sensitive to the antibiotics given to the patients from the beginning; however, both patients d ...19853898820
production and partial characterization of an elastolytic protease of vibrio vulnificus.conditions are described for the production of large amounts of an extracellular elastolytic protease by vibrio vulnificus. the yield of enzyme was maximal during the late exponential growth phase and was stable during the stationary growth phase in a medium composed of 2% proteose peptone and 1.5% nacl. the protease has a molecular weight of ca. 50,500 (estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis), an isoelectric point of ca. 5.8, and a ph optimum range against azocas ...19853902648
identification of vibrio vulnificus by indirect immunofluorescence.a rapid, sensitive, and specific indirect immunofluorescence (iif) procedure is described for identifying vibrio vulnificus. reference antisera were prepared by vaccinating rabbits with surface antigen preparations of v. vulnificus, and the antisera were examined for the ability to react with and serologically group 85 isolates of v. vulnificus grown in heart infusion broth, and to detect v. vulnificus in tissue specimens from mice experimentally infected with a virulent isolate of the bacterium ...19853905226
location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance.eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ...19853905805
location of polar substituents and fatty acyl chains on lipid a precursors from a 3-deoxy-d-manno-octulosonic acid-deficient mutant of salmonella typhimurium. studies by 1h, 13c, and 31p nuclear magnetic resonance.eight anionic disaccharide precursors of lipid a accumulate at 42 degrees c in 3-deoxy-d-manno-octulosonic acid-deficient temperature-sensitive mutants of salmonella typhimurium. these compounds comprise a series of lipids based on the minimal structure, o-[2-amino-2-deoxy-n2,o3-bis(3-hydroxytetradecanoyl)-beta-d-glucopyranos yl] -(1----6)-2-amino-2-deoxy-n2, o3-bis(3-hydroxytetradecanoyl)-alpha-d-glucopyranose 1,4'- bisphosphate (designated lipid iva) that differ from each other by the presence ...19853905805
mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12.the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ...19853911022
[effect of r plasmids on the growth characteristics of shigella sonnei and escherichia coli].revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: e. coli 15-3 and sh. sonnei 11-941 containing conjugative r plasmids differing in the set of the resistance markers. it was shown that the r plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. it was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of sh. sonnei 11-941 and ...19853911872
rapid microimmunodiffusion method with species-specific antiserum raised to purified antigen for identification of vibrio vulnificus.an antigen common to vibrio vulnificus strains, designated vva, was purified by ammonium sulfate precipitation, gel filtration, ion-exchange column chromatography, and preparative gel electrophoresis. the molecular weight of vva was 64,000 when estimated by gel filtration and 40,000 when measured by denaturing polyacrylamide gel electrophoresis. antiserum prepared against purified vva (anti-vva serum) did not agglutinate whole cells of v. vulnificus. therefore, vva was considered a possible inte ...19853918072
demonstration of a common antigen in sonicated cells for identification of vibrio vulnificus serotypes.ouchterlony immunodiffusion of sonicated vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. tandem two-dimensional immunoelectrophoretic analyses of v. vulnificus antigens from various strains revealed one fused precipitation line of identity. no fused precipitation lines were seen with other vi ...19853918076
the prostaglandin h synthase-dependent activation of 2-aminofluorene to products mutagenic to s. typhimurium strains ta98 and ta98nr. 19853925332
pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells.pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ...19853925713
synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells.human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ...19853931686
de novo expression of t cell markers on theileria parva-transformed lymphoblasts in cattle.we describe monoclonal antibodies that detect two new membrane antigens on bovine t cells. one molecule is only expressed on activated t cells and has a m.w. of 100,000. the other antigen is a glycoprotein that is precipitated as two bands of m.w. 150,000 and 158,000 and is expressed on a subpopulation of t cells and all myeloid cells. we show that when bovine lymphocytes are transformed by the protozoan parasite theileria parva, both antigens become expressed on the cell surface. the infected c ...19853934278
catalase enhances damage to dna by bleomycin-iron(ii): the role of hydroxyl radicals.bleomycin degrades dna under aerobic conditions when a ferrous salt is added. this reaction is enhanced by catalase and certain hydroxyl radical scavengers but inhibited by the addition of hydrogen peroxide. a ferricbleomycin complex is, however, stimulated by addition of hydrogen peroxide. these findings suggest that catalase removes hydrogen peroxide and in so doing prevents loss of ferrous ions and formation of hydroxyl radicals (oh.) by a fenton-type reaction. it further suggests that oh. ra ...19852409975
a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin.in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative.19852412878
relation of capsular materials and colony opacity to virulence of vibrio vulnificus.colonies which varied in opacity were isolated from the four strains of vibrio vulnificus. opaque and translucent colonial types of the strains were distinguished from the corresponding parent strains. variation in the opacity of colonies formed by each strain was accompanied by variation of capsular material formation, which was clarified by electron microscopy of the organisms stained with ruthenium red. the opaque-type colonies of the strains had capsular materials. on the other hand, three t ...19852578434
intestinal infection with mycobacterium avium in acquired immune deficiency syndrome (aids). histological and clinical comparison with whipple's disease.at endoscopy, a 30-year-old man with acquired immune deficiency syndrome (aids), kaposi's sarcoma, diarrhea, and unexplained malabsorption showed erythematous macular duodenal lesions consistent with whipple's disease by histology and electron microscopy. symptoms did not respond to tetracycline. subsequent cultures revealed systemic mycobacterium avium (m. avium) infection. tissue from this patient, from patients with whipple's disease and from a macaque with m. avium were compared. all contain ...19852580679
survival of smallpox virus. 19852857360
benzodiazepines enhance the muscimol-dependent activation of phospholipase a2 in glioma c6 cells.glioma c6 cells were incubated with [14c]arachidonate to label membrane phospholipids. muscimol, a selective gamma-aminobutyric acid a receptor agonist, but not (-)-baclofen, a selective gamma-aminobutyric acid b receptor agonist, stimulates [14c]arachidonate release from c6 cells as a result of hydrolysis of a small pool of phosphatidylcholine and phosphatidylethanolamine by phospholipase a2. this release is facilitated by diazepam and a number of other benzodiazepines such as flunitrazepam, me ...19852857784
immunochemical recognition of oxytocin by antiserum to l-prolyl-leucyl-glycine amide.antisera were raised in rabbits against pro-leu-gly-nh2 (plg)-bovine thyroglobulin conjugates. specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to sepharose beads. it was concluded that immunization with plg can raise antisera that are specific to oxytocin.19852859563
lymphatic transport of sodium cefoxitin in the presence of sodium 5-methoxysalicylate after injection into rat rectal connective tissue, femoral muscle and femoral vein.lymphatic uptake of sodium cefoxitin after injection into rectal connective tissue was greater than after injection into the femoral muscle of rats. coadministration with sodium 5-methoxysalicylate enhanced lymphatic drug uptake at both sites. this enhancement may be an indirect result of 5-methoxysalicylate's suppression of vascular permeation of the cefoxitin. an adjuvant-induced increase in lymphatic fluid flow may also be partially involved in the enhancement of cefoxitin lymphatic transport ...19852863362
[physiological and pharmacologic studies of the adrenal medulla by the measurement of plasma catecholamine levels].the new techniques for the assay of the plasma catecholamines: noradrenaline, dopamine and, most importantly, adrenaline, because of their sensitivity and precision, have provided new information about the physiology and the pharmacology of the adrenal medulla in animals and in man. the most effective of these new techniques are the radioenzymatic techniques. the activation of the adrenal medulla in the dog or the rat appears to be frequency-dependent by maintaining a fixed concentration ratio i ...19852863423
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...19852866480
neural and pavlovian influences on immunity.the traditional view that the nervous and immune systems are functionally independent (aside from general stress effects and autoimmune disorders of the nervous system) is being challenged by a new view that the nervous system regulates the activity of the immune system. if this is true, it should be possible to change the activity of the immune system by means of pavlovian conditioning, just as it is possible to condition other physiological events influenced by the autonomic nervous system or ...19852866484
restriction nuclease digestions driven to completion by escherichia coli rna polymerase and t4 gene 32 protein.restriction enzyme digestions of large scale dna preparations often do not go to completion. this is due to product inhibition by the newly generated ends of the digested dna. the addition of exogenous proteins that bind tightly to the free ends of dna or to single-stranded dna will relieve this inhibition. we show that a considerable savings on restriction nucleases can be attained by the addition of rna polymerase or t4 gene 32 protein in stoichiometric amounts to the newly produced dna ends.19852981211
herpes simplex virus and cytomegalovirus infections in burned patients.herpesvirus infections are commonly seen in immunosuppressed patients and may account for considerable morbidity and some mortality. we prospectively studied 52 patients with severe burn injuries in order to determine the prevalence of viral infections in this group of patients. serologic testing was done each week to diagnose primary and reactivation infections. twenty-seven of 52 patients (52%) became infected with either herpes simplex virus (hsv) or cytomegalovirus (cmv) or both. hsv infecti ...19852981325
conditioned taste aversion suppresses induction of delayed-type hypersensitivity immune reactions.conditioned taste aversion was induced in mice by pairing saccharin drinking with an intraperitoneal injection of lithium chloride, a toxic but nonimmunosuppressive drug. conditioned mice showed not only suppressed saccharin drinking but also a 75% reduction in the induction of delayed-type hypersensitivity immune responses to low doses of sheep erythrocytes. this effect was observed with doses of lithium chloride which had no effect of their own on immune functions. in addition, a reduction in ...19852987987
inflammatory myopathies: part 2. 19852988409
receptors for epidermal growth factor in the rat uterus.uterine membranes contain high affinity [dissociation constant (kd) = 0.36 nm], saturable binding sites for [125i]iodo-epidermal growth factor (egf). the binding of [125i] iodo-egf is specific since it is abolished by excess unlabeled egf but not by excess unlabeled insulin, fibroblast growth factor, or multiplication-stimulating activity. incubation of [125i] iodo-egf with uterine membranes, followed by chemical cross-linking with disuccinimidyl suberate and detergent extraction reveals a major ...19852988913
the biologic significance of the mixed lymphocyte kidney culture in humans.the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...19852996186
evaluation of a photolabile derivative of 1,25-dihydroxyvitamin d3 as a photoaffinity probe for 1,25-dihydroxyvitamin-d3 receptor in chick intestinal cytosol.we evaluated the viability of 1 alpha, 25-dihydroxyvitamin d3-3 beta-[n-(4-azido-2-nitrophenyl)glycinate] (1,25-(oh)2-d3-ang), an analog of 1 alpha, 25-dihydroxyvitamin d3 (1,25-(oh)2-d3) as a photoaffinity probe for 1,25-(oh)2-d3 receptor in chick intestinal cytosol. a competitive-binding assay revealed that chick intestinal cytosolic 1,25-(oh)2- d3 receptor bound to 1,25-(oh)2-d3-ang approximately 20-times less effectively than it did to 1,25-(oh)2-d3. irradiation of 1,25-(oh)2-d3- ang in the ...19852998355
effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection.the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ...19853000290
dimethylthiourea consumption reflects h2o2 concentrations and severity of acute lung injury.even though dimethylthiourea (dmtu) effectively scavenges o2 metabolites in vitro, it is often unclear if scavenging of o2 metabolites is the mechanism by which dmtu decreases tissue injury in biological models. since dmtu not only scavenges o2 metabolites but is also consumed in a dose-response manner following reaction with hydrogen peroxide (h2o2) in vitro, we wondered whether dmtu would also be consumed by o2 metabolites in biological systems and if dmtu consumption would then reflect o2 met ...19853001019
non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains.the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ...19853156400
the ada conference on increasing access to dental care. a report. 1985268525
drugs and the respiratory system. 1985261253
[intravenous administration of amikacin sulfate for the infections complicated with hematologic disorders (author's transl)]. 1985271749
[intermittent parasystole, parasystolic slow ventricular tachycardia and ventricular parasystoles: clinical observations and electrophysiological mechanisms]. 1985261915
will test tube babies spawn legal problems? 1985263190
[pretreatment in periodontal therapy].initial treatment is of central importance in dental interventions for marginal periodontal lesions. the hygienic phase must be successfully concluded before further measures of periodontal surgery or bigger reconstructions of any kind are undertaken. in this way optimal prognosis is achieved for all phases of therapy.1985271583
ultracentrifugal study of effect of sodium chloride on micelle size of fusidate sodium.apparent micellar molecular weights were determined with the antibiotic fusidate sodium by ultracentrifugation in varying counterion concentrations (na+). the effects of buffer salts, ph, sodium chloride concentration, and drug concentration were studied. the results strongly support the concept of the formation of primary micelles composed of five monomer units, followed by aggregation of the pentomers into larger micelles as salt concentration increases.1986239200
a hydrogen-deuterium exchange study of the amide protons of polymyxin b by nuclear-magnetic-resonance spectroscopy.1. proton magnetic resonance spectra at 270 mhz of polymyxin b, a cationic oligopeptide antibiotic, show the influence of the inorganic counteranion present in solution. 2. hydrogen-deuterium exchange rates for the amide protons are of two types, depending on whether the anion is monovalent or polyvalent. polyvalent anions catalyse the acid-catalysed reaction more than the monovalent anions. 3. the structure in solution was monitored using the proton signals of the amides, the phenylalanine arom ...1986203455
[pharyngeal tonsils, mouth breathing and facial development]. 1986268664
[responsibility for the patient's welfare]. 1986260995
differential initiation of translation of a single estrogen receptor mrna could explain some estradiol resistance cases.cell response to steroid stimulation is generally acknowledged to be mediated by an intracellular protein known as a receptor. response intensity is related to the affinity of the receptor and to the number of sites occupied by its specific ligand. although verified in the majority of experimental and clinical studies, certain phenomena of steroid hormone resistance would seem to challenge this assertion. application of gene molecular biology to determine the action mechanisms of steroid hormone ...19861306521
different effects of the ionophore a-23187 and d-glucose on 45ca2+ fluxes in isolated islets of ob/ob-mice.fluxes of 45ca2+ were studied in beta-cell rich islets of non-inbred ob/ob-mice, using lacl3 to wash out extra-cellular and superficially bound 45ca2+. the ionophore a-23187 (10 microm) increased the 45ca2+ uptake in islets both at 3 and 20 mm d-glucose, the effect being more pronounced after 10 min than after 120 min of incubation. in incubations for 120 min, 20 mm d-glucose induced a higher uptake of 45ca2+ than did a-23187. the ionophore enhanced the unidirectional efflux of 45ca2+ from prelo ...1986362823
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...19862942039
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...19862946570
ultrastructural changes in the sarcoplasmic reticulum in acute myocardial ischemia.we investigated the morphologic changes in the sarcoplasmic reticulum (sr) in acute myocardial ischemia, induced by occlusion of the coronary artery in the canine heart, by freeze-fracture of sr in situ and in vitro, and they were compared to the alterations in ca++-stimulated atpase activity and protein composition of the isolated sr. both sr in situ and the isolated sr exhibited typical intramembranous particles with diameters of 70 to 90 a in freeze-fracture replicas. the intramembranous part ...19862948034
second-generation cephalosporins. 19863081544
identification of n epsilon-carboxymethyllysine as a degradation product of fructoselysine in glycated protein.the chemistry of maillard or browning reactions of glycated proteins was studied using the model compound, n alpha-formyl-n epsilon-fructoselysine (ffl), an analog of glycated lysine residues in protein. incubation of ffl (15 mm) at physiological ph and temperature in 0.2 m phosphate buffer resulted in formation of n epsilon-carboxymethyllysine (cml) in about 40% yield after 15 days. cml was formed by oxidative cleavage of ffl between c-2 and c-3 of the carbohydrate chain and erythronic acid (ea ...19863082871
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...19863085110
effect of nifedipine on cerebral high-energy phosphates after cardiac arrest and resuscitation in the rat.we studied the effect of nifedipine, a calcium entry blocker, on the recovery of cerebral adenosine triphosphate (atp), creatine phosphate (cp), and lactate levels following resuscitation from cardiac arrest. using the cardiac arrest and resuscitation model of de garavilla, babbs, and tacker with an arrest time of eight minutes, 76% of the animals arrested were resuscitated with an average intermittent abdominal compression-cpr time of 3.3 minutes. rats were assigned randomly to the following gr ...19863085559
evidence that some membrane ligands modulate the plasmalemma fluidity of endothelial cells in culture.the effect of some membrane ligands on the plasmalemmal fluidity of endothelial cells from bovine aorta in culture was investigated. the ligands used were: cationic ferritin (pi 8.5), soybean agglutinin, concanavalin a, wheat germ agglutinin, as well as glutaraldehyde at different concentrations. the fluidity probe employed was 1,6-diphenyl-1, 3, 5-hexatriene (dph) and the parameter determined to quantify the fluidity was fluorescence steady-state anisotropy. the optimum time interval required b ...19863088620
nasopharyngeal carriage of neisseria meningitidis in the contacts of index cases of meningococcal meningitis. 19863091676
[a saved case of vibrio vulnificus infection with shock, dic and necrotizing fasciitis]. 19863097209
protamine sulfate-induced enzyme secretion from rabbit neutrophils.protamine sulfate induces enzyme secretion from rabbit neutrophils. enzyme secretion is mainly due to exocytosis but, depending on the experimental conditions, a small amount of cytolysis may occur. as compared with stimulation of neutrophil functions by other activators, protamine sulfate-induced enzyme release by exocytosis is a relatively slow process and is not accompanied by a marked activation of the metabolic burst. for optimal exocytosis, extracellular ca2+ is required, but there is stil ...19863098680
rhamnetin-3-o-neohesperidoside, a new flavonoid from the leaves of derris trifoliata. 19863509960
potentiation of hemorrhage-evoked catecholamine release by prior blood loss in cats.the effect of prior blood loss on the plasma catecholamine response to acute hemorrhage (h) was assessed in alpha-chloralose-urethane anesthetized cats. animals sustained an initial h period of 0 (samples only), 10, or 20% h total blood volume. ninety minutes after reinfusion of the shed blood, all animals sustained a rapid 20% h. the catecholamine and arterial pressure responses to this second 20% h were assessed every 2 min for 20-min duration. plasma norepinephrine increased modestly in the 0 ...19863510559
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