Publications

impact of an urban effluent on antibiotic resistance of riverine enterobacteriaceae and aeromonas spp.	in order to evaluate the impact of an urban effluent on antibiotic resistance of freshwater bacterial populations, water samples were collected from the arga river (spain), upstream and downstream from the wastewater discharge of the city of pamplona. strains of enterobacteriaceae (representative of the human and animal commensal flora) (110 isolates) and aeromonas (typically waterborne bacteria) (118 isolates) were selected for antibiotic susceptibility testing. most of the aeromonas strains (7 ...	2000	10618213
[gene cloning and expression of prolyl endopeptidase from aeromonas punctata].	prolyl endopeptidase gene was cloned from aeromonas punctata subsp. punctata(st-78-3-3) using activity screening method and the 3.3 kb dna fragment containing pep gene was sequenced. dna sequence from 20-2092 bp was orf region coding 690 amino acids of prolyl endopeptidase. it was a new pep gene through gene search. the genetic engineered e. coli bl21/pgem-pep overexpressing recombinant aeromonas punctata prolyl endopeptidase(appep) was constructed. cultured in yh medium, the expressed appep was ...	2000	12548992
aeromonas caviae as a cause of cholecystitis.		2000	11217277
high-efficiency expression of prolyl endopeptidase from aeromonas punctata subsp. punctata in escherichia coli.	the open reading frame (orf) of prolyl endopeptidase gene from aeromonas punctata subsp. punctata (appep) was amplified by pcr in three parts. the amplified dna segments were ligated to form the complete appep gene, and then cloned into expression vectors pbl (temperature inducible) and pkkh (iptg inducible), respectively. after induction, the expression amounts of recombinant appep in bl21/pkkh-pep and bl21/pbl-pep were about 30% of the total bacterial proteins, and the enzyme activities were 1 ...	2000	12098799
[purification and characterization of recombinant aeromonas punctata prolyl endopeptidase].	the study of down-stream techniques of recombinant aeromonas punctata prolyl endopeptidase (appep) was presented here. high cell-density fermentation of e. coli bl21/pkkh-pep in nbs bioflo 3000 5 l fermentor was achieved, the final cell density was 22.5 g (dcw)/l after 14 h cultivation, the yield of appep expressed in soluble protein was 3.0 g per litter broth. after sonication, the supernatant of free cell extract was purified by ammonium sulfate fractionation, high performance q sepharose ff, ...	2000	11059278
[cultivation optimization of prolyl endopeptidase and high cell density fermentation].	engineered e. coli bl21/pgem-pep could constitutively express recombinant prolyl endopeptidase from aeromonas punctata, which was extremely effected by culture conditions, so fermentation conditions were optimized to obtain it's high expression level. firstly, the stability of bl21/pgem-pep was investigated, then, culture temperature, ph, time, medium were optimized in shake flaskd. the l9(3(4)) orthogonal experiment confirmed that shaker speed, ph, culture temperature, culture time had high deg ...	2000	10976323
two-week postnatal examination of infants.		2000	87641
[is it unreasonable to administer glucocorticoids to patients with neurogenic gastrointestinal hemorrhage? (author's transl)].		2000	91986
distinction between endocervical and endometrial adenocarcinoma with immunoperoxidase staining of carcinoembryonic antigen in routine histological tissue specimens.	the differential diagnosis of endocervical and endometrial adenocarcinomas can be improved by the demonstration of carcinoembryonic antigen (cea) in tissue by means of immunoperoxidase staining. tissue from 131 (80%) of 163 patients with endocervical adenocarcinoma but only 11 (8%) of 137 patients with endometrial adenocarcinoma was cea-positive. the commonest exceptions were endocervical mesonephroid adenocarcinomas (which were cea-negative) and endometrial adenosquamous carcinomas (which were ...	2000	91890
computed tomographic evaluation of the breast.	experience with a dedicated breast ct scanner (general electric ct/m) using a contrast medium enhancement technique indicates that ct is superior to mammography, thermography, or physical examination for diagnosing both benign and malignant mammary disease especially in dense, thick, or fibrocystic breasts. ct is capable of diagnosing totally unsuspected early miniature carcinomas. it can identify and differentiate potential precancerous lesions from benign fibrocystic disease, and is the diagno ...	2000	98990
symbolic play in language-impaired and normal children.		2001	95236
family life cycle resource center in the elementary school.		2001	98495
[recent acquisitions concerning the relationships between vasoactive amines and delayed hypersensitivity].		2001	93298
virulence factors of clinical isolates of aeromonas caviae.	the mechanism of pathogenicity of 13 aeromonas caviae strains isolated from fecal specimens of children with diarrhea was examined. isolates possessed several virulence agents. six of 13 isolates produced cholera-like toxin, all strains exhibited adhesive ability and secreted catecholate siderophores. six isolates were able to grow in heat-inactivated serum. production of hemolysins was not detected. the isolates had relatively high values of ld50 in a mouse model, which suggests that there is n ...	2001	11830944
characterization of 13 kda granule-associated protein in aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria.	analysis of native poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [p(3hb-co-3hhx)] inclusions from aeromonas caviae fa440 revealed that orf1 (a 348-bp gene located immediately upstream of phac(ac)) encodes a 13-kda granule-associated protein, which was referred to as phap(ac). several recombinant strains of a. caviae were constructed and conducted to analyze their pha-producing abilities. a transconjugant of fa440 harboring additional copies of phapcj(ac) genes accumulated p(3hb-co-3hhx) copolyes ...	2001	11749166
motility and the polar flagellum are required for aeromonas caviae adherence to hep-2 cells.	aeromonas caviae is increasingly being recognized as a cause of gastroenteritis, especially among the young. the adherence of aeromonads to human epithelial cells in vitro has been correlated with enteropathogenicity, but the mechanism is far from well understood. initial investigations demonstrated that adherence of a. caviae to hep-2 cells was significantly reduced by either pretreating bacterial cells with an antipolar flagellin antibody or by pretreating hep-2 cells with partially purified f ...	2001	11401962
g561 site-directed deletion mutant chitinase from aeromonas caviae is active without its 304 c-terminal amino acid residues.	a g561 mutant of the aeromonas caviae chitinase chia was made by pcr site-directed deletion mutagenesis in order to study the role of the 304 c-terminal amino acid residues of chia in the enzymatic hydrolysis of chitin. the recombinant chiag561 encoded on a 1.6-kb dna fragment of a. caviae chia was expressed in a heterologous escherichia coli host using the pet20b(+) expression system. the his-tag-affinity-purified recombinant chiag561 had a calculated molecular mass of 63,595 da, which was cons ...	2001	11357514
enhancement of the virulence of aeromonas caviae diarrhoeal strains by serial passages in mice.	thirteen clinical aeromonas caviae isolates from the faeces of 13 children with mild to severe diarrhoea were tested for enhancement of mouse lethality, adhesion ability, siderophore and cholera toxin cross-reactive (ctc) factor production, by four consecutive passages through mice by intraperitoneal injection of a. caviae suspensions. the passaged a. caviae strains were re-isolated from monomicrobic cardiac blood samples and inocula were prepared for the next passage. all a. caviae isolates pos ...	2001	11289515
role of flm locus in mesophilic aeromonas species adherence.	the adherence mechanism of aeromonas caviae sch3n to hep-2 cells was initially investigated through four mini-tn5 mutants that showed a 10-fold decrease in adherence. these mutants lost motility, flagella, and their lipopolysaccharide (lps) o antigen (o-ag). three genes, flmb-neua-flmd, were found to be interrupted by the transposon insertions; additionally, two other genes, one lying upstream (flma) and one downstream (neub), were found to be clustered in the same operon. while the flma and flm ...	2001	11119490
isolation and identification of motile aeromonas species from chicken.	in this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile aeromonas species. according to analysis findings, motile aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. the distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. aeromonas hydrophila was isolat ...	2001	11765602
effect of high oxygen modified atmosphere packaging on microbial growth and sensorial qualities of fresh-cut produce.	the application of high oxygen atmospheres (hoa) (i.e. > 70% o2) for packaging ready-to-eat vegetables was evaluated as an alternative technique for low o2 equilibrium modified atmosphere (ema) packaging (3% o2-5% co2-balance n2) for respiring products. comparative experiments between both techniques were performed in-vitro and in-vivo. typical spoilage causing microorganisms (pseudomonas fluorescens, candida lambica), the moulds botrytis cinerea, aspergillus flavus and the opportunistic psychro ...	2001	11789938
an expression release system for heterogeneous proteins in e.coli mediated by the kil gene.	an expression release system for heterogeneous proteins in escherichia coli mediated by the colicin release gene(kil gene) was constructed. the system is based on the ability of the kil protein to release periplasmic proteins into the growth medium. beta-lactamase, an e.coli periplasmic protein, and prolyl endopeptidase (pep), a periplasmic protein of aeromonas punctata subsp. punctata were used as report proteins. commonly, these two proteins are seldom released into the growth medium. the resu ...	2001	12040395
identification of a 43-kda outer-membrane protein as an adhesin in aeromonas caviae.	aeromonas spp. are associated with intestinal and extra-intestinal infections. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interactions involved in the adherence of a. caviae isolates ae56, ae391 and ae398 to hep-2 cells. all strains expressed high levels of aggregative adherence. maximum adhesion occurred with bacteria grown at 22 degrees c, but transmission electron microscopy did not reveal the presence of fimbrial structures on ...	2001	11289516
characterization of the pro-aminopeptidase from aeromonas caviae t-64.	the pro-aminopeptidase from aeromonas caviae t-64 (pro-apac) had maximal activity at 60 degrees c and was more stable than mature apac at temperature up to 65 degrees c for 1 hour. the ph stability of pro-apac ranged from 4.0 to 8.0, which is broader than the range for the mature apac. the kcat/km of pro-apac was 1.4% to 24% of that of mature apac.	2001	11302179
cloning, expression, and characterization of a family 52 beta-xylosidase gene (xysb) of a multiple-xylanase-producing bacterium, aeromonas caviae me-1.	a lambda phage genomic library of aeromonas caviae me-1, a multiple-xylanase-producing bacterium, was screened for xylan degradation activities. we isolated one clone, b65, which had weak xylanase activity, by the dns method, but gave no visible bands on zymogram assay using sds-xylan-page. based on tlc analyses of enzymatic products and some glycosidase assays using p-nitrophenyl substrates, we established that pb65 encodes a beta-xylosidase gene. in the nucleotide sequence analysis, we found a ...	2001	11330658
mesophilic aeromonads in wild and aquacultured freshwater fish.	numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ...	2001	11348001
comparison of the antimicrobial tolerance of oxytetracycline-resistant heterotrophic bacteria isolated from hospital sewage and freshwater fishfarm water in belgium.	the aim of this study was to investigate the relationship between antimicrobial tolerance and taxonomic diversity among the culturable oxytetracycline-resistant (ot(r)) heterotrophic bacterial population in two belgian aquatic sites receiving wastewater either from human medicine or from aquaculture. the study of ot(r) heterotrophs and mesophilic aeromonas spp. allowed comparison of tolerance data at the intergenus as well as at the intragenus level. in total, 354 independently obtained ot(r) is ...	2001	11403391
virulence factors in vibrios and aeromonads isolated from seafood.	thirty-one isolates from seafood, identified as aeromonas hydrophila (7), aeromonas caviae (11), vibrio parahaemolyticus (3), vibrio fluvialis (5), vibrio alginolytictus (3), vibrio metschnikovii (1) and vibrio damsela (1), were tested for possible virulence factors including extracellular hydrolytic enzymes, haemolysins, cytotoxins (vero and hep-2 cells) and adherence ability (hep-2 cells). all the a. hydrophila strains were beta-haemolytic and produced cytotoxins as well as one strain of v. fl ...	2001	11497085
a secreted aminopeptidase of pseudomonas aeruginosa. identification, primary structure, and relationship to other aminopeptidases.	using leucine-p-nitroanilide (leu-pna) as a substrate, we demonstrated aminopeptidase activity in the culture filtrates of several pseudomonas aeruginosa strains. the aminopeptidase was partially purified by deae-cellulose chromatography and found to be heat stable. the apparent molecular mass of the enzyme was approximately 56 kda; hence, it was designated ap(56). heating (70 degrees c) of the partially purified aminopeptidase preparations led to the conversion of ap(56) to a approximately 28-k ...	2001	11533066
helicobacter pylori and other enteric bacteria in freshwater environments in mexico city.	helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear.	2001	11578764
detection of aeromonas caviae in the common housefly musca domestica by culture and polymerase chain reaction.	aeromonas caviae has been implicated in diarrhoeal disease of livestock and humans. the potential role of houseflies in the epidemiology of this pathogen was investigated by examining the prevalence of a. caviae in houseflies collected from two south carolina farms and one restaurant. isolation was accomplished by culture of flies in alkaline peptone water followed by identification with aeromonas-specific pcr using novel primers (apw-pcr). all isolates cultured from houseflies were identified a ...	2001	11811891
crystallization and preliminary x-ray analysis of (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.	dimeric (r)-specific enoyl-coenzyme a (coa) hydratase from aeromonas caviae catalyzes the hydration of trans-2-enoyl-coas with carbon lengths of 4-6 to yield their corresponding (r)-3-hydroxyacyl-coas and is essential for polyhydroxyalkanoate (pha) biosynthesis. the enzyme has been crystallized by vapour diffusion against a reservoir solution containing 20% polyethylene glycol 4000, 5% 2-propanol and 20 mm hepes ph 7.0 at 298 k. crystals belong to the monoclinic space group c2, with unit-cell pa ...	2001	11134939
[a new practice system for heart-lung resuscitation (dräger-laerdal].		2001	93277
preliminary experience with the helifix electrode for transvenous atrial implantation.	a helifix electrode was inserted transvenously in the right atrial appendage for temporary atrial stimulation in three patients. the low stimulation threshold as well as the excellent stability of the electrode encouraged us to proceed to permanent implantation of this lead in thirteen patients. the lead was introduced through the saphenous vein in twelve patients and through the cephalic vein in one patient. five patients had sinoatrial dysfunction and five had bradycardia-tachycardia syndrome; ...	2001	95313
[does one have to be aggressive in the surgical management of diverticular disease of the colon (author's transl)].	a series of 61 consecutive cases with diverticular disease and its complications is presented. fourteen cases with uncomplicated diverticular disease and 3 with massive gi tract hemorrhage underwent an uneventful (segmental) resection. of 6 cases operated for fistula formation 1 died. surgical therapy for abscessformation, peritonitis and "pseudo-tumoral" obstruction (38 cases) carried a high mortality (26.3%). the application of a one-staged aggressive and curative surgical approach lowers the ...	2001	93359
[photocoagulation of late complications of retinal venous occlusions (analysis of 22 cases treated for 1 to 5 1/2 years)].		2001	93028
purification, characterization and amplification of a 1.8 kbp fragment of xylanase 5 from aeromonas caviae w-61.	aeromonas caviae w-61 produces multiple extracellular xylanases, the xylanases 1, 2, 3, 4, and 5. in this study, we purified and characterized the xylanase 5 of a. caviae w-61, and amplified a part of xylanase 5 gene (xyn5). the purified xylanase 5 was found to be a single polypeptide with molecular mass of 140 kda. it was an endo-beta-1,4-xylanase showing optimum temperature 40 degrees c and optimum ph 6.0. xylobiose, xylotriose, xylotetrose, xylopentose, xylohexose and a small amount of xylose ...	2002	22905388
renewed voluntary spirit needed, says blue cross;.		2002	139559
[postoperative esophageal stenosis (role of the laryngologist)].		2002	153373
[2 cases of sudden death of twins with idiopathic cardiomegaly].		2002	129892
in vitro antimicrobial susceptibility of clinical isolates of aeromonas caviae, aeromonas hydrophila and aeromonas veronii biotype sobria.		2002	11909850
predictive modelling for packaging design: equilibrium modified atmosphere packages of fresh-cut vegetables subjected to a simulated distribution chain.	the impact of temperature fluctuations in a simulated cold distribution chain, typical of commercial practice, was investigated on both the microbial and sensorial quality of equilibrium modified atmosphere (ema) packaged minimally processed vegetables. the internal o2 concentration of the designed packages could be predicted for the different steps of the simulated distribution chain by applying an integrated mathematical system. the internal atmosphere in the packages remained in its aerobic r ...	2002	11934040
enhanced accumulation and changed monomer composition in polyhydroxyalkanoate (pha) copolyester by in vitro evolution of aeromonas caviae pha synthase.	by in vitro evolution experiment, we have first succeeded in acquiring higher active mutants of a synthase that is a key enzyme essential for bacterial synthesis of biodegradable polyester, polyhydroxyalkanoate (pha). aeromonas caviae fa440 synthase, termed phac(ac), was chosen as a good target for evolution, since it can synthesize a pha random copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate [p(3hb-co-3hhx)] that is a tough and flexible material compared to polyhydroxybutyrate (phb) hom ...	2002	11976116
in vitro stepwise autoprocessing of the proform of pro-aminopeptidase processing protease from aeromonas caviae t-64.	pa protease (pro-aminopeptidase processing protease) is an extracellular zinc metalloprotease produced by the gram-negative bacterium aeromonas caviae t-64. the 590-amino-acid precursor of pa protease is composed of a putative 19-amino-acid signal sequence, a 165-amino-acid n-terminal propeptide, a 33 kda mature protease domain and an 11 kda c-terminal propeptide. the proform of pa protease, which was produced as inclusion bodies in escherichia coli, was subjected to in vitro refolding. it was r ...	2002	11983417
lateral flagella of aeromonas species are essential for epithelial cell adherence and biofilm formation.	mesophilic aeromonas strains express a single polar flagellum in all culture conditions and produce lateral flagella on solid media. such hyperflagellated cells demonstrate increased adherence. nine lateral flagella genes, lafa-u for aeromonas hydrophila, and four aeromonas caviae genes, lafa1, lafa2, lafb and fliu, were isolated. mutant characterization, nucleotide and n-terminal sequencing demonstrated that the a. hydrophila and a. caviae lateral flagellins were almost identical, but were dist ...	2002	11985716
engineering of ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from fructose and solid-state properties of the copolymer.	recombinant ralstonia eutropha capable of producing poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) copolymer [p(3hb-co-3hhx)] from fructose was engineered by introduction of genes for crotonyl-coa reductase (ccr) from streptomyces cinnamonensis (ccrsc) and for pha synthase and (r)-specific enoyl-coa hydratase from aeromonas caviae (phac-jac). in this recombinant strain, c6-acyl-coa intermediates were provided via beta-ketothiolase-mediated elongation of butyryl-coa, which was generated from croto ...	2002	12005535
synthesis of a novel class of polyhydroxyalkanoates in arabidopsis peroxisomes, and their use in monitoring short-chain-length intermediates of beta-oxidation.	the poly[(r)-3-hydroxyalkanoate] (pha) synthase gene (phac(ac)) of aeromonas caviae fa440 was modified by adding a peroxisome targeting signal encoding the last 10 amino acids at the carboxyl-terminal of spinach glycolate oxidase. the modified gene was introduced into arabidopsis thaliana plants by agrobacterium-mediated transformation. the transgenic arabidopsis plant expressed the introduced gene and its protein, and it accumulated pha in its tissues. gas chromatography-mass spectrometry analy ...	2002	12040103
lateral flagella and swarming motility in aeromonas species.	swarming motility, a flagellum-dependent behavior that allows bacteria to move over solid surfaces, has been implicated in biofilm formation and bacterial virulence. in this study, light and electron microscopic analyses and genetic and functional investigations have shown that at least 50% of aeromonas isolates from the species most commonly associated with diarrheal illness produce lateral flagella which mediate swarming motility. aeromonas lateral flagella were optimally produced when bacteri ...	2002	11751834
the role of the fatty acid beta-oxidation multienzyme complex from pseudomonas oleovorans in polyhydroxyalkanoate biosynthesis: molecular characterization of the fadba operon from p. oleovorans and of the enoyl-coa hydratase genes phaj from p. oleovorans and pseudomonas putida.	in order to investigate the role of the putative epimerase function of the beta-oxidation multienzyme complex (fadba) in the provision of (r)-3-hydroxyacyl-coa thioesters for medium-chain-length polyhydroxyalkanoate (pha(mcl)) biosynthesis, the fadba(po) operon of pseudomonas oleovorans was cloned and characterized. the fadba(po) operon and a class-ii pha synthase gene of pseudomonas aeruginosa were heterologously co-expressed in escherichia coli to determine whether the putative epimerase funct ...	2002	12115060
the role of the n-terminal propeptide of the pro-aminopeptidase processing protease: refolding, processing, and enzyme inhibition.	pro-aminopeptidase processing protease (pa protease) is an extracellular zinc metalloprotease produced by aeromonas caviae t-64 and it is classified as m04.016 according to the merops database. the precursor of pa protease consists of four regions; a signal peptide, an n-terminal propeptide, a c-terminal propeptide, and the mature pa protease. the in vitro refolding of the intermediate pro-pa protease containing the c-terminal propeptide (mc) was investigated in the presence and absence of the n ...	2002	12147230
in vivo evolution of the aeromonas punctata polyhydroxyalkanoate (pha) synthase: isolation and characterization of modified pha synthases with enhanced activity.	in vivo random mutagenesis of the polyhydroxyalkanoate (pha) synthase gene from aeromonas punctata was performed employing the mutator strain escherichia coli xl1-red. about 200,000 mutants were screened on nile red-containing medium and five mutants with enhanced fluorescence were selected. four of these mutants exhibited enhanced in vivo and in vitro pha synthase activity. mutant m1, which carried the single mutation f518i, showed a five-fold increase in specific pha synthase activity, whereas ...	2002	12172613
biochemical identification and numerical taxonomy of aeromonas spp. isolated from environmental and clinical samples in spain.	to study the phenotypic characteristics of aeromonas spp. from environmental and clinical samples in spain and to cluster these strains by numerical taxonomy.	2002	12174040
effect of superatmospheric oxygen packaging on sensorial quality, spoilage, and listeria monocytogenes and aeromonas caviae growth in fresh processed mixed salads.	atmospheres with o2 levels higher than 70 kpa have recently been suggested as an innovation to modified atmosphere packaging (map) for fresh processed vegetables to maintain sensory quality and safety. in the present work, mixed vegetable salad collected from a commercial processing plant and stored with the map technique was studied. two gas mixtures were actively generated by using an initial o2 concentration of 95 kpa and combined with two plastic films. the low-barrier film permeability for ...	2002	12380740
type ii topoisomerase quinolone resistance-determining regions of aeromonas caviae, a. hydrophila, and a. sobria complexes and mutations associated with quinolone resistance.	most aeromonas strains isolated from two european rivers were previously found to be resistant to nalidixic acid. in order to elucidate the mechanism of this resistance, 20 strains of aeromonas caviae (n = 10), a. hydrophila (n = 5), and a. sobria (n = 5) complexes, including 3 reference strains and 17 environmental isolates, were investigated. fragments of the gyra, gyrb, parc, and pare genes encompassing the quinolone resistance-determining regions (qrdrs) were amplified by pcr and sequenced. ...	2002	11796341
vector potential of houseflies for the bacterium aeromonas caviae.	houseflies, musca domestica linnaeus (diptera: muscidae), have been implicated as vectors or transporters of numerous gastrointestinal pathogens encountered during feeding and ovipositing on faeces. the putative enteropathogen aeromonas caviae (proteobacteria: aeromonadaceae) may be present in faeces of humans and livestock. recently a. caviae was detected in houseflies by pcr and isolated by culture methods. in this study, we assessed the vector potential of houseflies for a. caviae relative to ...	2002	12109714
virulence factors of a. caviae strains isolated from acute diarrheic disease in cuba.	fifty aeromonas caviae strains from intestinal infection in different cuban provinces were identified by the aerokey ii method and virulence factors were investigated. the strains did not produce haemolysins but other exoenzymes such as proteases, lipases, and dnases; additionally, all isolates adhered to the hep-2 cell line by the carrello method and this did not correlate with other virulence factors presence which demonstrates that the haemolysin phenotypic expression is not necessary for the ...	2002	17061509
the bender gestalt: use of clinical judgment versus recall scores in prediction in huntington's disease.		2002	130390
a symposium on the chronic pain syndrome in problem low back cases. psychiatric evaluation and feasibility of psychiatric therapy.		2002	127024
atpase activity and light scattering of acto-heavy meromyosin: dependence on atp concentration and on ionic strength.	1. the dependence on atp concentration of atpase activity and light scattering decrease of acto-hmm could be described at very low ionic strength by one hyperbolic adsorption isotherm with a dissociation constant of 3 x 10(-6)m. hence the increase of atp ase activity was paralleled by a decrease in light scattering. at higher values of ionic strength atpase activity stopped rising before hmm was completely saturated with atp. higher ionic strength prevented atpase activity from further increasin ...	2002	126582
[medical topics. nursing and rehabilitation of aged invalid patients].		2002	127875
[comparative sanitary and hygienic evaluation of rubbers intended fordeep-well electric pumps].		2002	134925
[occupational exogenous siderosis of the skin (author's transl)].		2003	151604
conduits--indications, problems, and future use.		2003	420058
conduits--indications, problems, and future use.		2003	420058
the consequences of nullosomy for a chromosomal region affecting cyclic amp phosphodiesterase activity in drosophila.	a study of drosophila nullosomic for chromomere 3d4 shows that this region of the genome is necessary for male fertility, normal female fertility and normal oogenesis. males nullosomic for 3d4 lack normal, motile sperm. females nullosomic for this region exert a maternal influence on their progeny which results in a diversity of imaginal defects. the observation that chromomere 3d4 is the most probable locus for a chromosomal region which affects camp phosphodiesterase activity, and which may co ...	2003	193764
[glomerular lesions and hepatopathies].		2003	152421
purification of cytochrome b from neurospora crassa and other sources.		2003	152379
group 1 beta-lactamases of aeromonas caviae and their resistance to beta-lactam antibiotics.	the contribution of beta-lactamase production to beta-lactam antibiotic resistance was examined in an aeromonas caviae mutant strain, selected in vitro by cefotaxime and derived from a wild-type strain isolated in our laboratory from crude sewage. both strains produced beta-lactamase. the mutant strain (ac7m) produced beta-lactamase constitutively, in contrast to the parental strain (ac7), which was inducible by cefoxitin. ac7m was regarded as a mutant from ac7, which over-expressed beta-lactama ...	2003	12795407
cloning, expression, and cell surface localization of paenibacillus sp. strain w-61 xylanase 5, a multidomain xylanase.	we have shown that a xylan-degrading bacterium, w-61, excretes multiple xylanases, including xylanase 5 with a molecular mass of 140 kda. here, we emend the previously used classification of the bacterium (i.e., aeromonas caviae w-61) to paenibacillus sp. strain w-61 on the basis of the nucleotide sequence of the 16s rrna gene, and we clone and express the xyn5 gene encoding xylanase 5 (xyn5) in escherichia coli and study the subcellular localization of xyn5. xyn5 encodes 1,326 amino acid residu ...	2003	14660338
the c-terminal module of chi1 from aeromonas caviae cb101 has a function in substrate binding and hydrolysis.	the chitinase gene chi1 of aeromonas caviae cb101 encodes an 865-amino-acid protein (with signal peptide) composed of four domains named from the n-terminal as an all-beta-sheet domain chin, a triosephosphate isomerase (tim) catalytic domain, a function-unknown a region, and a putative chitin-binding domain (chbd) composed of two repeated sequences. the n-terminal 563-amino-acid segment of chi1 (chi1deltaadeltachbd) shares 74% identity with chia of serratia marcescens. by the homology modeling m ...	2003	14635132
the processing of high-molecular-weight xylanase (xyne, 110 kda) from aeromonas caviae me-1 to 60-kda xylanase (xyne60) in escherichia coli and purification and characterization of xyne60.	a xylanase gene (xyne) encoding xyne (110 kda) was cloned from a lambda phage genomic library of aeromonas caviae me-1 which is a multiple-xylanase-producing bacterium. upon nucleotide sequence analysis, we found that xyne comprises 2823 by and encodes a protein of 941 amino acid residues (104,153 da), which was similar to endo-beta-1,4-xylanases which are categorized to glycosyl hydrolase family 10. an escherichia coli transformant that harbored pxed30 carrying xyne produced 110-, 84-, 72-, and ...	2003	16233373
a cytoplasmic xylanase (xynx) of aeromonas caviae me-1 is released from the cytoplasm to the periplasm by osmotic downshock.	aeromonas caviae me-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, samia cynthia pryeri. one of the xylanases produced by a. caviae me-1, xynx (38 kda, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. generally, xylanases are extracellular or cell surface enzymes. however, xynx is not exported to the extracellular fluid by a. caviae me-1 and an escherichia coli transformant ...	2003	16233445
processing of xyne (110-kda) of aeromonas caviae me-1 to 72-kda xylanase in escherichia coli transformant.	an extracellular 72-kda xylanase from an escherichia coli transformant that harbored pxed30 carrying xyne of aeromonas caviae me-1 was purified by extraction following sds-page to electrophoretic homogeneity. the analysis of n-terminal 10 amino acid residues (gly-ala-arg-ala-gln-ala-ala-ala-asp-val) revealed a 72-kda product derived by the cleavage of gly26-gly27 at the n-terminal region of 110 kda xyne. the 72-kda xylanase from a. caviae me-1 was found to have the same sequence as that of the n ...	2003	16233547
[synthesis of copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyhexanoate by aeromonas hydrophila wq and its molecular basis].	aeromonas hydrophila wq isolated from lake water was found to be able to synthesize polyhydroxyalkanoates (pha) copolymer consisting of 3-hydroxybutyrate (hb) and 3-hydroxyhexanoate (hhx) (phbhhx). lauric acid was found to be the most suitable carbon source for cell growth and phbhhx accumulation. the bacteria accumulated 49% phbhhx containing 6% hhx in terms of cell dry weight when grown on lauric acid for 72 h. 42% phbhhx consisting of 14% hhx was obtained with 5 g/l glucose and 10 g/l lauric ...	2003	16276907
enteropathogenic activity and invasion of hep-2 cells by aeromonas caviae clinical isolates.	twenty aeromons caviae isolates from stool of children with diarrhea symptoms were examined for virulence-associated properties: production of cytotoxic and cytotonic toxins, and invasive ability. most of a. caviae strains were cytotoxic to vero and cho cells and produced cytotonic toxins which caused elongation of cho cells. moreover, five of a. caviae strains revealed invasive ability towards hep-2 cells.	2003	14743980
[discordancies between classical and api 20e microtest biochemical identification of vibrio and aeromonas strains].	the present study reveals some discordancies of diagnosis considering vibrionaceae strains when tested by comparison with api 20e (biomerieux) system and classical biochemical reactions, aspects already mentioned in the literature by other authors. if any misidentification has not an important impact (e.g. identification of aeromonas schubertii as a. sobria), others as misidentification of aeromonas as vibrio strains or the failure to recognize vibrio strains raise major epidemiological problems ...	2003	15341329
crystal structure of the (r)-specific enoyl-coa hydratase from aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.	the (r)-specific enoyl coenzyme a hydratase ((r)-hydratase) from aeromonas caviae catalyzes the addition of a water molecule to trans-2-enoyl coenzyme a (coa), with a chain-length of 4-6 carbons, to produce the corresponding (r)-3-hydroxyacyl-coa. it forms a dimer of identical subunits with a molecular weight of about 14,000 and is involved in polyhydroxyalkanoate (pha) biosynthesis. the crystal structure of the enzyme has been determined at 1.5-a resolution. the structure of the monomer consist ...	2003	12409309
molecular typing of aeromonas isolates in natural mineral waters.	a total of 103 isolates of aeromonas spp. were obtained over a 3-year period from a natural mineral water and from surface streams located within the boundaries of the watershed of the natural mineral water wells and were typed by macrorestriction analysis of genomic dna with xbai and by pulsed-field gel electrophoresis. all aeromonas caviae isolates from the natural mineral water belonged to the same clone, and an analogous clonal identity was found among aeromonas hydrophila isolates. these tw ...	2003	12514065
the genetics of glycosylation in gram-negative bacteria.	in recent years there has been a dramatic increase in reports of glycosylation of proteins in various gram-negative systems including neisseria meningitidis, neisseria gonorrhoeae, campylobacter jejuni, pseudomonas aeruginosa, escherichia coli, caulobacter crescentus, aeromonas caviae and helicobacter pylori. although this growing list contains many important pathogens (reviewed by benz and schmidt [mol. microbiol. 45 (2002) 267-276]) and the glycosylations are found on proteins important in pat ...	2003	12586395
general function of n-terminal propeptide on assisting protein folding and inhibiting catalytic activity based on observations with a chimeric thermolysin-like protease.	pro-aminopeptidase processing protease (pa protease) is a thermolysin-like metalloprotease produced by aeromonas caviae t-64. the n-terminal propeptide acts as an intramolecular chaperone to assist the folding of pa protease and shows inhibitory activity toward its cognate mature enzyme. moreover, the n-terminal propeptide strongly inhibits the autoprocessing of the c-terminal propeptide by forming a complex with the folded intermediate pro-pa protease containing the c-terminal propeptide (mc). ...	2003	12589825
cloning, dna sequence, and expression of aeromonas caviae ws7b chitinase gene.	a chitinase-producing bacterium, designated ws7b, was isolated from a soil sample obtained from a black-pepper plantation on bangka island, indonesia. fatty-acid methyl-ester analysis indicated that the isolate was aeromonas caviae. a chitinase gene from ws7b was cloned in a puc19-based plasmid vector, but without its natural promoter. the complete nucleotide sequence of the gene was determined, and the structural gene consisted of a 2748-bp region encoding 864 amino acids. dna sequence analysis ...	2003	12611264
bacteriocin-like substance (bls) production in aeromonas hydrophila water isolates.	30 aeromonas hydrophila water isolates were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria, including food-borne pathogens. a. hydrophila showed antibacterial activity against one or more indicator microorganisms, but the activity emerged only with non-phylogenetically related genera or species. in particular all a. hydrophila showed antibacterial activity against one or more of the ...	2003	12644237
influence of polarisation and differentiation on interaction of 43-kda outer-membrane protein of aeromonas caviae with human enterocyte-like caco-2 cell line.	it has been recognised that adherence and invasion to host cells are important steps in the pathogenesis of entero-pathogenic bacteria, including aeromonas caviae. however, the virulence factors of a. caviae remain, for the most part, poorly known. this study examined the interaction of a. caviae isolates to caco-2 cells in different polarisation and differentiation conditions. the adherence of a. caviae may be related to accessibility of host cell basolateral receptors. aggregative a. caviae is ...	2003	12684708
enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) via manipulating the fatty acid beta-oxidation pathway in e. coli.	acyl-coa dehydrogenase gene (yafh) of escherichia coli was expressed together with polyhydroxyalkanoate synthase gene (phac(ac)) and (r)-enoyl-coa hydratase gene (phaj(ac)) from aeromonas caviae. the expression plasmids were introduced into e. coli jm109, dh5 alpha and xl1-blue, respectively. compared with the strains harboring only phac(ac) and phaj(ac), all recombinant e. coli strains harboring yafh, phac(ac) and phaj(ac) accumulated at least four times more poly(3-hydroxybutyrate-co-3-hydroxy ...	2003	12694916
factors associated with the adherence and biofilm formation by aeromonas caviae on glass surfaces.	knowledge on factors of aeromonas caviae promoting the formation of biofilms on surfaces.	2003	12752817
sequence analysis and biochemical characterisation of chromosomal cav-1 (aeromonas caviae), the parental cephalosporinase of plasmid-mediated ampc 'fox' cluster.	aeromonas caviae cip 74.32 was resistant to amoxicillin, ticarcillin and cephalothin, and susceptible to cefoxitin, cefotaxime, ceftazidime, aztreonam and imipenem. this strain produced a cephalosporinase (pi 7.2) and an oxacillinase (pi 8.5). the cephalosporinase gene cav-1 was cloned and sequenced. unlike a. caviae donor, escherichia coli pnce50 transformant producing cav-1 beta-lactamase was resistant to cefoxitin. the deduced protein sequence cav-1 contained 382 amino acids, and shared >96% ...	2003	12757951
lateral flagella are required for increased cell adherence, invasion and biofilm formation by aeromonas spp.	two types of flagella are responsible for motility in mesophilic aeromonas strains. a polar unsheathed flagellum is expressed constitutively that allows the bacterium to swim in liquid environments and, in media where the polar flagellum is unable to propel the cell, aeromonas express peritrichous lateral flagella. recently, southern blot analysis using a dna probe based on the aeromonas caviae sch3n lateral flagellin gene sequence showed a good correlation between strains positive for the dna p ...	2003	12855171
aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis b.	gram-negative bacilli of the genus aeromonas are widespread in aquatic environments and can be responsible for human infections. although aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, aeromonas septicaemia remains an uncommon finding, often associated with serious underlying disease and predominantly related to the species aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae. here, a case of a. veronii biovar ve ...	2003	12867570
alteration of chain length substrate specificity of aeromonas caviae r-enantiomer-specific enoyl-coenzyme a hydratase through site-directed mutagenesis.	aeromonas caviae r-specific enoyl-coenzyme a (enoyl-coa) hydratase (phaj(ac)) is capable of providing (r)-3-hydroxyacyl-coa with a chain length of four to six carbon atoms from the fatty acid beta-oxidation pathway for polyhydroxyalkanoate (pha) synthesis. in this study, amino acid substitutions were introduced into phaj(ac) by site-directed mutagenesis to investigate the feasibility of altering the specificity for the acyl chain length of the substrate. a crystallographic structure analysis of ...	2003	12902277
[water electrolyte excretion disorders during dry erythroderma: hypersecretion of antidiuretic hormone?].		2003	161489
treatment of spasmodic torticollis with dorsal column stimulation.		2004	303442
[choroid plexus carcinoma with metastasis to bone (author's transl)].	a very rare case of the choroid plexus carcinoma of the left lateral ventricle with metastasis to bone was reported. a two-month-old baby was suffering from irritability and vomiting. her vital signs became progressively worse. on admission she showed an increased head circumference and meningeal irritation signs. an emergency tapping of the right lateral ventricle at the anterior fontanel revealed the bloody csf and an opening pressure of over 700 mm of water. all laboratory findings were negat ...	2004	492463
support for 208 planning.		2004	522207
ultrasound, angiography and fine needle aspiration biopsy in diagnosis of renal neoplasms.	the diagnostic approach to a renal mass including ultrasound, angiography and fine needle aspiration biopsy is reviewed on the basis of 23 cases. the value of the combination of these methods is emphasized especially in tumors which appear without echoes at ultrasround and avascular-hypovascular at angiography. the findings are correlated and some differential diagnostic problems discussed as well as the possible risk of spreading tumor cells at fine needle aspiration biopsy.	2004	525406
[features of psychopathologic disorders in schizophrenia complicated by psychogenic reactions].	in order to reveal psychopathological disturbances in schizophrenia appearing under the influence of psychogenic factors, 145 schizophrenic patients with attack-like progressive and continuous forms of development, accompanied by psychogenic reactions were examined. the significant changes in the clinical picture of such conditions expressed in atypical and fragmentary psychopathological signs indicate a deep influence of psychogenic reactions on the pathogenesis of the schizophrenic process. th ...	2004	525123
children's literature on death and dying.		2004	375165
homogeneous immunoglobulins in the serum of irradiated and bone marrow reconstituted mice: the role of thymus and spleen.	the influence of thymectomy and splenectomy on the frequency and class distribution of homogeneous immunoglobulins (h-ig) in serum was studied in lethally irradiated (dba/2 x c57bl/rij)f1 mice reconstituted with syngeneic bone marrow. during four follow-up periods in the first 9 months after transplantation, the sham-operated controls and splenectomized animals developed transient h-ig in an average frequency of 14.2 and 15.7% respectively. there were no marked differences in the incidence of h- ...	2004	387578
increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of saccharomyces cerevisiae through modification of the beta-oxidation cycle.	short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from aeromonas caviae and ralstonia eutropha in the peroxisomes. the quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward r-3-hydroxybutyryl coenzyme a.	2004	15345460
detection, integration and persistence of aeromonads in water distribution pipe biofilms.	the occurrence of aeromonas spp. within biofilms formed on stainless steel (ss), unplasticized polyvinyl chloride (upvc) and glass (gl) substrata was investigated in modified robbins devices (mrd) in potable (mrd-p) and recycled (mrd-r) water systems, a biofilm reactor (br) and a laboratory-scale pipe loop (pl) receiving simulated recycled wastewater. no aeromonads were isolated from the mrd-p whereas 3-10% of ss and upvc coupons (mean 3.85 cfu cm(-2) and 12.8 cfu cm(-2), respectively) were aero ...	2004	15387132
an unusually high level of quinolone resistance associated with type ii topoisomerase mutations in quinolone resistance-determining regions of aeromonas caviae isolated from diarrhoeal patients.	we examined 158 strains belonging to different aeromonas species isolated from hospitalized acute diarrhoea cases for susceptibility to quinolones. compared to other species, a high percentage of the a. caviae strains were resistant to ciprofloxacin and norfloxacin. based on mic values, 6 a. caviae strains were selected and the nucleotide sequences for the quinolone-resistant-determining regions (qrdrs) of gyra, gyrb and parc genes were analysed. in resistant strains, double mutations (ser(83)-- ...	2004	15567276