Publications

intraoperative use of doppler to detect successful obliteration of carotid-cavernous fistulas. technical note.	the authors describe their use of a doppler flow detector in the treatment of carotid-cavernous fistulas to monitor the ocular bruit, the clinical sign usually used to detect obliteration of the fistula. in seven procedures the doppler ultrasonic flow detector has yielded satisfactory proof that the flow sounds were no longer audible, and that the surgery was successful.	2002	127022
[some features of the organization of hospital medical care of the adult population of a large city].		2002	130684
sexuality and the severely disabled person.		2002	127531
[echocardiography: iii--myocardiopathies].		2003	161966
genotype and hormonal phenotype in nonclassical 21-hydroxylase deficiency.	in nonclassical steroid 21-hydroxylase deficiency, the genotype may be represented as a homozygous mild (nonclassical) form of the 21-hydroxylase defect or as a compound heterozygote, with one severe (classical) and one mild (nonclassical) 21-hydroxylase deficiency allele. we examined hormone levels in patients with nonclassical 21-hydroxylase deficiency in whom pedigree analysis and/or hla linkage disequilibrium allowed unequivocal identification of the respective haplotypes as either classical ...	2003	3023431
[a comparative study of acid mucopolysaccharides of paranasal sinus mucous membrane in normal and chronic sinusitis (author's transl)].		2003	162040
comparative sensitivity of different serological tests for seromonitoring and surveillance of edwardsiella tarda infection of indian major carps.	different serological tests viz. indirect elisa, indirect blocking elisa, competitive elisa and serum agglutination tests were evaluated to detect antibodies against edwardsiella tarda in naturally infected fish sera for seromonitoring and epizootiological studies. approximately 66.6, 62.5, 57.6 and 16.6% of the field sera samples were found to be positive by indirect elisa, competitive elisa, indirect blocking elisa and serum agglutination test, respectively. the percentage of serum samples pos ...	2003	12969654
high antigenic cross-reaction among the bacterial species responsible for diseases of cultured freshwater fishes and strategies to overcome it for specific serodiagnosis.	antigenic sharing among the most commonly bacterial pathogens such as aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens of indian major carps has been studied using immunological reactions such as cross-agglutination, disc diffusion and indirect enzyme linked immunosorbent assay (elisa). the data were analysed using statistical analysis (sas), version 6.12. the results showed high antigenic similarities among the bacterial whole cells, whole cell lysates, somatic 'o' antigens, ...	2003	12581749
functional genomics approach to the identification of virulence genes involved in edwardsiella tarda pathogenesis.	edwardsiella tarda is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. here, we report the identification of 14 virulence genes of pathogenic e. tarda that are essential for disseminated infection, via a genome-wide analysis. we screened 490 alkaline phosphatase fusion mutants from a library of 450,000 tnphoa transconjugants derived from strain ppd130/91, using fish as an infection model. compared to the wild type, 15 mutants show ...	2003	12595451
maternal colonization and neonatal sepsis caused by edwardsiella tarda.	a case of neonatal sepsis caused by edwardsiella tarda, a bacterium usually associated with freshwater ecosystems, is described. the infant's mother was immersed in lake water during the sixth month of pregnancy and had vaginal and gastrointestinal colonization with the same strain of e tarda as the infant at the time of delivery. this case suggests that maternal exposures to contaminated bodies of water during pregnancy may represent a risk to newborns.	2003	12612287
combination effects of cephalexin and gentamicin on edwardsiella tarda and streptococcus iniae.	the objective of this study was to determine the in vitro activity of cephalexin-gentamicin combination by a microbroth chequerboard technique against clinical isolates of edwardsiella tarda and streptococcus iniae. gentamicin was shown more susceptible than cephalexin against both bacteria. the effect of cephalexin-gentamicin combination against both bacteria represented additive interaction. the combination even showed synergic interaction (22%) against e. tarda, with a fic index of <0.5 as a ...	2003	12842330
puerperal intrauterine infection caused by edwardsiella tarda.	edwardsiella tarda infection is uncommon and has been reported to be associated with pet reptiles and the ingestion of raw fish. the majority of the infections occur as gastrointestinal disorders in immunocompromised hosts. we report a case of puerperal intrauterine e. tarda infection in a patient with no history of predisposing illness or of the recent ingestion of raw fish; this is the first such report. the patient complained of high fever, lower abdominal pain, and increasing amounts of malo ...	2003	14691656
sanitary and bacteriological aspects of sewage treatment.	a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ...	2003	15058814
a major catalase (katb) that is required for resistance to h2o2 and phagocyte-mediated killing in edwardsiella tarda.	edwardsiella tarda causes haemorrhagic septicaemia in fish and gastro- and extra-intestinal infections in animals including humans. resistance to phagocyte-mediated killing is one of the virulence factors of ed. tarda. the authors' previous studies using tnphoa transposon mutagenesis indicated that katb mutants derived from the strain ppd130/91 are at least 1.6 log higher in ld50 values than the wild-type strain. these findings suggest the involvement of catalase (katb) in ed. tarda pathogenesis ...	2003	12949187
identification of a 19.3-kda protein in mrha-positive edwardsiella tarda: putative fimbrial major subunit.	the hemagglutinating properties of edwardsiella tarda isolated from fish were investigated. hemagglutination of e. tarda was not inhibited by d-mannose but was strongly inhibited by fetuin and n-acetylneuraminic acid. extraction of hemagglutinating activity from bacterial cells was achieved using n-octyl-beta-d-thioglucoside (notg), and the notg extracts were fractionated by sucrose density gradient ultracentrifugation. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the fr ...	2003	13129618
cpg motif in synthetic odn primes respiratory burst of olive flounder paralichthys olivaceus phagocytes and enhances protection against edwardsiella tarda.	effects of synthetic cytidine-phosphate-guanosine (cpg) oligodeoxynucleotide (odn) on respiratory burst activity of olive flounder (paralichthys olivaceus) head-kidney phagocytes and on protection against lethal infection with edwardsiella tarda were investigated. phagocytes precultured with a cpg odn showed significantly higher chemiluminescence (cl) responses than phagocytes precultured with guanosine-phosphate-cytidine (gpc) odn or culture medium alone (control) at all concentrations. superna ...	2003	14524500
resection of a massive arteriovenous malformation of the head and neck.	we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case.	2003	154679
bacterial enteritis and the development of the larval digestive tract in olive flounder, paralichthys olivaceus (temminck & schlegel).	three bacterial isolates obtained from diseased olive flounder larvae, paralichthys olivaceus, were identified as vibrio ichthyoenteri based on the results of phenotypic characterization and 16s rrna gene sequencing studies. bacterial enteritis was reproduced in 16 and 22 days post-hatch (dph) larvae by administering brine shrimp nauplii, artemia salina, dosed with the environmental isolates and reference strains of v. ichthyoenteri. to investigate the effect of the disease on development of the ...	2004	15357708
identification of quorum-sensing signal molecules and the luxri homologs in fish pathogen edwardsiella tarda.	edwardsiella tarda is a gram-negative bacterium that causes septicaemia in fish and serious damage to the aquaculture industry. the virulence factors of this pathogen and control mechanisms of the expression of virulence genes have not yet been clearly elucidated. a number of gram-negative pathogenic bacteria have a quorum-sensing system. these bacteria produce n-acyl-l-homoserine lactone (ahl) that they use them as a quorum-sensing signal molecule. in this study, we found that e. tarda isolated ...	2004	16233705
determination of the bacterial pathogen edwardsiella tarda in fish species by capillary electrophoresis with blue light-emitting diode-induced fluorescence.	high-performance capillary electrophoresis (hpce) has been applied to the identification, separation, and quantitation of intact bacteria. we demonstrate that a pathogen (edwardsiella tarda) which causes systemic infection in commercially important fish species can be rapidly identified and determined (< 10 min) after direct injection into fish fluid by ce blue light-emitting diode (led)-induced fluorescence. syto 13 (488 nm/509 nm), a cell-permeable green nucleic acid stain, was used to stain t ...	2004	15472969
a conserved 37 kda outer membrane protein of edwardsiella tarda is an effective vaccine candidate.	an effective vaccine against edwardsiella tarda has not been reported, one of main reasons is the variation in its serotypes. this study aimed to develop an effective vaccine against different serotypes of e. tarda. a conserved 37 kda outer membrane protein (omp) of e. tarda was obtained by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page). it showed comprising several proteins by two-dimensional (2d) page analysis and showed a high similarity to glyceraldehyde-3-phosphate d ...	2004	15308366
polymicrobial bacteremia caused by escherichia coli, edwardsiella tarda, and shewanella putrefaciens.	edwardsiella tarda, a member of enterobacteriaceae, is found in freshwater and marine environments and in animals living in these environments. this bacterium is primarily associated with gastrointestinal diseases, and has been isolated from stool specimens obtained from persons with or without clinical infectious diseases. shewanella putrefaciens, a saprophytic gram-negative rod, is rarely responsible for clinical syndromes in humans. debilitated status and exposure to aquatic environments are ...	2004	15605912
sensitive and rapid detection of edwardsiellosis in fish by a loop-mediated isothermal amplification method.	here we report a rapid and sensitive method (using loop-mediated isothermal amplification [lamp]) for the diagnosis of edwardsiellosis, a fish disease caused by edwardsiella tarda, in japanese flounder. a set of four primers was designed, and conditions for the detection were optimized for the detection of e. tarda in 45 min at 65 degrees c. no amplification of the target hemolysin gene was detected in other related bacteria. when the lamp primers were used, detection of edwardsiellosis in infec ...	2004	14711699
pharmacokinetics of sulfadimethoxine and ormetoprim in a 5:1 ratio following intraperitoneal and oral administration, in the hybrid striped bass (morone chrysops x morone saxitalis).	selected pharmacokinetic parameters for sulfadimethoxine and ormetoprim, administered in a 5:1 ratio, via the oral and intraperitoneal (i.p.) routes were determined in the hybrid striped bass (morone chrysops x morone saxitalis). plasma concentrations of both drugs were determined by high-performance liquid chromatography. a first-order one-compartment model adequately described plasma drug disposition. the elimination half-lives for sulfadimethoxine following i.p. and oral administration were 2 ...	2004	14995959
pathogenesis and inflammatory response to edwardsiella tarda infection in the zebrafish.	the zebrafish (danio rerio) is a widely used model for developmental biology, neurobiology, toxicology, and genetic disease. recently, the zebrafish has been recognized as a valuable model for infectious disease and immunity. in this study the pathogenesis and inflammatory cytokine response of zebrafish to experimental edwardsiella tarda infection was characterized. in challenge experiments, zebrafish embryos were susceptible to infection by immersion. adult fish were susceptible to challenge by ...	2004	15752547
use of proteomics to identify novel virulence determinants that are required for edwardsiella tarda pathogenesis.	edwardsiella tarda is an important cause of haemorrhagic septicaemia in fish and also of gastro- and extraintestinal infections in humans. using a combination of comparative proteomics and tnphoa mutagenesis, we have identified five proteins that may contribute to e. tarda ppd130/91 pathogenesis. lowered protein secretion, impaired autoaggregation and the absence of six proteins were observed only in three highly attenuated mutants when cultured in dulbecco's modified eagle medium (dmem). five o ...	2004	15228535
the future of an illusion--the illusion of the future and historic dialogue on the value of religion between oskar pfister and sigmund freud.		2004	400630
denture insertion.		2004	321279
cardiac arrest and resuscitation: defibrillators and defibrillation.		2004	343996
absence of toxicity in cimetidine overdosage.		2004	427404
folic acid and reproduction.		2004	535198
high-resolution ultrasonography of scrotal pathology.	a prototype high-resolution (10-mhz) real-time ultrasonic system was used to study 22 patients who were ultimately proved to have intra- and extratesticular tumors, scrotal fluid collections, and inflammatory processes. in 5 of 6 patients with primary testicular masses, there were areas of decreased echogenicity within the testis when compared with surrounding normal tissue. in general, the method was highly effective in differentiating and characterizing testicular and extratesticular processes ...	2004	441377
[1st orthopedic textbook of the world for the use of the physician. on the 200th anniversary of the birth of johann christian gottfried jörg].		2004	395945
[pseudogout].		2004	520893
percutaneous placement of inferior vena cava filters.	pulmonary embolism is a serious and difficult problem. many approaches for the prevention of recurrent pulmonary embolism have been tried. percutaneous placement of inferior vena cava filters is an easy, safe, available and well established procedure for the prevention of pulmonary embolism. the authors review the indications for use of ivc filters, and they review the main filters available in terms of ease of use, the physical characteristics, the technique of introduction, the efficacy and mo ...	2004	1492602
nutritional supplementation and the outcome of pregnancy. ii. visual habituation at 15 days.	colombian women at risk of mild-to-moderate malnutrition were enrolled in a health care program and randomly assigned into nutritional supplementation and control groups at the beginning of the third trimester of preganancy. one hundred unsupplemented and 144 supplemented infants were tested at 15 days of age: a 2x2 checkerboard was presented eight times followed by a single presentation of a 6x6 checkerboard. unsupplemented infants showed less initial attention followed by slower habituation an ...	2004	420136
diagnosis of edwardsiella tarda infection in oyster toadfish (opsanus tau) held at the marine resources center.		2004	27690585
stroke rehabilitation. part i. general considerations.		2005	1214691
part ii: the photic environment of clear tropical seas during the day.		2005	1166605
[mitral valve prolapse. diagnostic exploration with non-invasive technics].		2005	548101
effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant.		2005	544179
procollagen biosynthesis in embryonic chick arteries.		2005	1168592
[the sjögren-gougerot syndrome].	the sjögren-gougerot syndrome represents a rare disease of the collagenous tissue characterized by the three symptoms xerophtalmy, xerostomy, polyarthritis. women after the menopause are the most affected. oral symptoms addup to diverse dermatological manifestations. the bilateral parotidic swelling and systemic complications accompany the disease, and the immunological disorder is coupled with hypergammaglobulinemia. prognosis is very reserved and treatment delicate.	2005	1064976
molecular cloning of the gyra gene and characterization of its mutation in clinical isolates of quinolone-resistant edwardsiella tarda.	knowing the entire sequence of the gene encoding the dna gyrase subunit a (gyra) of edwardsiella tarda could be very useful for confirming the role of gyra in quinolone resistance. degenerate primers for the amplification of gyra were designed from consensus nucleotide sequences of gyra from 9 different gram-negative bacteria, including escherichia coli. with these primers, dna segments of the predicted size were amplified from the genomic dna of e. tarda and then the flanking sequences were det ...	2005	16408842
enteric fever-like illness caused by infection with citrobacter amalonaticus.	'enteric fever' is a potentially fatal, severe systemic disease, which is encountered worldwide. traditionally, enteric fever refers to a bacteremic illness caused by members of certain salmonella serotypes, notably: salmonella typhi, a gram-negative bacterium, and to a lesser extent, salmonella paratyphi a, b and c. in addition, other non-salmonella organisms may produce a syndrome clinically indistinguishable from "enteric fever". brucella sp., campylobacter sp., edwardsiella tarda, enterobact ...	2005	16083226
the family of serratia type pore forming toxins.	the serratia marcescens hemolysin represents the prototype of a growing family of pore forming toxins. the available bacterial genome sequences reveal serratia hemolysin homologues in additional species. however, only s. marcescens hemolysin has been studied in great molecular detail. this family of toxins has nothing in common with the pore forming toxins of e. coli type (rtx toxins), the staphylococcus aureus alpha-toxin or the thiol activated toxin of group a beta-hemolytic streptococci (stre ...	2005	16101433
analysis of 16s-23s intergenic spacer regions of the rrna operons in edwardsiella ictaluri and edwardsiella tarda isolates from fish.	to analyse interspecies and intraspecies differences based on the 16s-23s rrna intergenic spacer region (isr) sequences of the fish pathogens edwardsiella ictaluri and edwardsiella tarda.	2005	16108808
genetic loci of major antigenic protein genes of edwardsiella tarda.	seven antigenic proteins of edwardsiella tarda were identified by using a rabbit polyclonal antiserum. four of these proteins also reacted with a japanese flounder antiserum. the amino acid sequences had identity to lipoproteins, periplasmic proteins, and exported and secreted proteins with roles in transport of metabolites across the cell membrane, stress response, and motility. these genes and their products are useful for developing dna or recombinant subunit vaccines to control edwardsiellos ...	2005	16151172
effect of climate on the bacteriology of chronic suppurative otitis media.	we evaluated the correlation between the microbial content of chronic suppurative otitis media (csom) and regional climatic parameters.	2005	16190100
structural characterization of the o-polysaccharide antigen of edwardsiella tarda mt 108.	edwardsiella tarda, a gram-negative bacterium, is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. the lipopolysaccharide produced by the fish pathogenic strain e. tarda mt 108 was isolated and the structure of its antigenic o-polysaccharide component determined by the application of chemical analyses, high-resolution 1d and 2d nuclear magnetic resonance spectroscopy, and mass spectrometry. the polysaccharide was found to be a pol ...	2005	15620670
functional characterization of full-length tlr3, irak-4, and traf6 in zebrafish (danio rerio).	recently, the zebrafish, danio rerio, has been recognized as a useful model for infectious disease and immunity. the toll-like receptor (tlr) family is an evolutionarily conserved component of the innate immune system that responds to specific pathogen-associated molecular patterns (pamps) during an infection. this study reports the identification and characterization of a full-length orthologue of mammalian tlr3, and the key tlr pathway signaling molecules irak-4 and traf6 in the zebrafish. seq ...	2005	15829296
role of type iii secretion in edwardsiella tarda virulence.	edwardsiella tarda is a gram-negative enteric bacterium affecting both animals and humans. recently, a type iii secretion system (ttss) was found in ed. tarda. such systems are generally used by bacterial pathogens to deliver virulence factors into host cells to subvert normal cell functions. genome-walking was performed from the eseb and esrb genes (homologues of salmonella sseb and ssrb, respectively) identified in previous studies, to determine the sequences of the ttss. thirty-five orfs were ...	2005	16000720
extraintestinal manifestations of edwardsiella tarda infection.	summary edwardsiella tarda, a member of the family enterobacteriaceae, is a rare human pathogen. gastroenteritis is the most frequently reported manifestation of e. tarda infection. in contrast, extraintestinal infection with e. tarda has rarely been reported. this study made a retrospective case and microbiological data review of patients with extraintestinal e. tarda infections to further understand this disease. this study retrospectively reviewed the charts of all isolates of e. tarda cultur ...	2005	16033613
carriage by the housefly (musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in misurata, libya.	using standard microbiological procedures, bacteria that are potentially pathogenic to humans were isolated from 150 houseflies collected in the libyan city of misurata (50 flies each from the central hospital, streets and abattoir). salmonella spp., yersinia enterocolitica and edwardsiella tarda were isolated from flies collected on the streets and in the abattoir but not from those collected in the hospital. shigella sonnei was detected in just one fly, which was collected in the abattoir. of ...	2005	16297293
loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens.	fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. early diagnosis plays a vital role in management of fish and shellfish diseases. traditionally, various biochemical and serological tests have been used for fish disease diagnosis. however, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. polymerase chain reaction and probe-based nucleic acid detec ...	2005	16302951
vaccine efficacy of recombinant gapdh of edwardsiella tarda against edwardsiellosis.	thirty-seven kilodalton glyceraldehyde-3-phosphate dehydrogenase (gapdh) of edwardsiella tarda was suggested to be an effective vaccine candidate against e. tarda infection in previous research. for developing a vaccine, obtaining gapdh in large quantities is necessary. in this study, we determined the complete nucleotide sequence of the gene that encodes gapdh of e. tarda, and overexpressed the gapdh of e. tarda by using the escherichia coli expression system. we immunized japanese flounder wit ...	2005	16034203
regulation of a type iii and a putative secretion system in edwardsiella tarda by esrc is under the control of a two-component system, esra-esrb.	edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and gastro- and extraintestinal infections in humans. a type iii secretion system (ttss) and a putative secretion system (evp) have been found to play important roles in e. tarda pathogenesis. our previous studies suggested that the ttss and evp gene clusters were regulated by a two-component system of esra-esrb. in the present study, we characterized another regulator, esrc, which showed significan ...	2005	15972502
[open study of clopirac in the treatment of non articular rheumatism (author's transl)].		2005	1084347
protective effects and mechanisms of a probiotic bacterium lactobacillus rhamnosus against experimental edwardsiella tarda infection in tilapia (oreochromis niloticus).	in recent years, probiotics, especially lactic acid bacteria, have been used as dietary supplements to protect fish from various infections. here, we examined the protective effects of lactobacillus rhamnosus against experimental edwardsiella tarda infection in tilapia (oreochromis niloticus). cumulative mortality was significantly lower in probiotic-supplemented fish than in control fish. in a histopathological survey, pyogranulomatous responses were observed at an earlier stage and to a greate ...	2006	16870266
edwardsiella tarda septicemia with underlying multiple liver abscesses.	edwardsiella tarda has recently been described as a member of the family enterobacteriaceae. the genus edwardsiella contains three species; e. hoshinae, e. ictaluri and e. tarda. edwardsiella tarda is the only species which has been recognised as pathogenic to humans, especially in those with an underlying disease. the most common presentation is watery diarrhoea. extra intestinal infections have been reported infrequently. humans seem to be infected or colonised with edwardsiella through ingest ...	2006	17694959
protection of tilapia (oreochromis mosambicus) from edwardsiellosis by vaccination with edwardsiella tarda ghosts.	the vaccine potential of edwardsiella tarda ghosts produced by gene e mediated lysis was investigated using tilapia (oreochromis mosambicus). tilapia immunized with e. tarda ghosts (etg) and formalin killed e. tarda (fkc) vaccines showed significantly higher serum agglutination titers than control fish. fish immunized with etg showed no significant differences with fish immunized with fkc in serum agglutination titers, but showed significantly higher bactericidal activity than fish immunized wit ...	2006	16226892
liver abscess caused by edwardsiella tarda biogroup 1 and identification of its epidemiological triad by ribotyping.	two clinical isolates and an environmental isolate of edwardsiella tarda biogroup 1 (etb1), recovered from liver pus, the stool specimen and from the pond water of the village of the patient, diagnosed to have liver abscess, were found to be identical by protein fingerprinting and ribotyping. it can be construed that the pond water served as the source of infection. the epidemiological triad of the agent (etb1), host (the patient) and environment (pond water) was thus established. this is the fi ...	2006	16687868
transgenic zebrafish expressing chicken lysozyme show resistance against bacterial diseases.	we established a transgenic zebrafish strain expressing chicken lysozyme gene under the control of the japanese flounder keratin gene promoter, and investigated its resistance to a pathogenic bacterial infection. to generate the lysozyme transgenic construct, japanese flounder keratin promoter was linked to both the hen egg white (hew) lyoszyme gene and green fluorescence protein (gfp) gene used as a selection marker for the transgenic strains, in a recombinant plasmid. the recombinant plasmid w ...	2006	16779653
characterization of edwardsiella tarda strains isolated from turbot, psetta maxima (l.).	the biochemical, serological and molecular characteristics of a group of 21 edwardsiella tarda strains isolated from turbot, psetta maxima, in two different areas of europe were analysed and compared with a total of 13 strains of this bacterial species with different geographical and host origins. all the turbot isolates were biochemically identical to the e. tarda strains included as reference. the use of different techniques including microagglutination, dot blot and western blot of lipopolysa ...	2006	16948704
pathogenicity of edwardsiella tarda to olive flounder, paralichthys olivaceus (temminck & schlegel).	the ld50 and cytotoxic and enzymatic activities of both cells and extracellular products (ecps) of eight edwardsiella tarda strains were determined and their bacterial superoxide dismutase gene (sodb) and catalase gene (katb) were sequenced. strains were also examined for their ability to resist the immune responses of olive flounder, paralichthys olivaceus. ld50 values of strains (fsw910410, ke1, 2, 3, 4, 5 and 6) in olive flounder ranged between 10(2.5) and 10(5.3) cfu (colony forming units) p ...	2006	17026669
intracellular replication of edwardsiella tarda in murine macrophage is dependent on the type iii secretion system and induces an up-regulation of anti-apoptotic nf-kappab target genes protecting the macrophage from staurosporine-induced apoptosis.	edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. here we show that intracellular replication of e. tarda in murine macrophages is dependent on the type iii secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic nf-kappab target genes. the wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type iii ...	2006	17049431
identification and characterization of a myeloid differentiation factor 88 (myd88) cdna and gene in japanese flounder, paralichthys olivaceus.	the interleukin-1 receptor/toll-like receptor (il-1r/tlr) superfamily signaling involves myeloid differentiation factor 88 (myd88) that acts as an important adapter protein. a japanese flounder (paralichthys olivaceus) myd88 (jfmyd88) cdna and gene were cloned, and found to have lengths of 1.5 and 3.01 kb, respectively. the orf encodes 285 amino acids that contain a death domain and a toll/il-1 receptor domain. the gene is composed of 5 exons and 4 introns. the jfmyd88 gene is highly expressed i ...	2006	16412507
pathology of edwardsiella tarda infection in turbot, scophthalmus maximus (l.).	macroscopic and histopathological changes in cultured turbot, scophthalmus maximus (l.), in spain caused by infection with edwardsiella tarda are described. eye tumefaction, inflammation, haemorrhages, ascites and the presence of a purulent fluid were the main macroscopic lesions observed. histopathological lesions were found in the kidney, spleen and liver. in the kidney and spleen these were characterized by a severe apostematous inflammatory reaction, with a large number of abscesses. the liv ...	2006	16436119
occurrence of edwardsiella tarda in wild european eels anguilla anguilla from mediterranean spain.	pure cultures of edwarsiella tarda were isolated from body ulcers and internal organs of wild european eels caught in a mediterranean freshwater coastal lagoon (albufera lake, valencia, spain) over a 1 yr period. overall, the e. tarda isolation rate from wild eels was 9%, but this increased to 22.8% in diseased individuals. all 22 e. tarda isolates belonged to the 'wild-type' biogroup of the species and were virulent for eels (lethal dose that kills 50% of exposed individuals [ld50 dose]: 10(4.8 ...	2006	17240756
the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita.	the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ...	2006	16293422
unraveling edwardsiella tarda pathogenesis using the proteomics approach.		2006	16929682
a case of empyema caused by edwardsiella tarda.	in december 2003, a 57-year-old-man was diagnosed as having a hepatic tumor for which he had a hepatectomy. on pathology, the hepatic tumor biopsy specimen was diagnosed as malignant lymphoma. in february 2005, the patient was referred to our hospital because of fever and chest pain. a right pleural effusion was seen on chest x-ray. microscopic examination of the stained pleural fluid revealed many neutrophils and gram-negative rods, and edwardsiella tarda was cultured from the pleural effusion ...	2006	16620999
occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in spain.	to survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions of fresh marine fish.	2006	16478492
edwardsiella tarda as a cause of postdysenteric ulcerative colitis.		2006	15772809
effect of multiple injections of beta-glucan on non-specific immune response and disease resistance in labeo rohita fingerlings.	the purpose of this study was to determine if multiple injections of different dosages of beta-glucan derived from barley would enhance the immune response and disease resistance against infections due to opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of beta-glucan suspension in phosphate-buffered saline at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l ...	2006	16039142
schizencephaly: correlation of clinical findings with mr characteristics.	to correlate clinical outcome with the size and location of clefts in patients with schizencephaly.	2006	1595498
tryptophan-deficient diet and amino acid drink deplete plasma tryptophan and induce a relapse of depression in susceptible patients.		2006	1524722
[the significance of the determination of catecholamines and their metabolites in neuroblastomas].	a method for the determination of catecholamines and their metabolites in the urine was established. the specimens were previously cleaned by ion exchangers and the components were separated by a 'reversed phase' ionic pair chromatography, determined by an electrochemical 'wall-jet'-detector. this method was applied in order to find a diagnostic marker in case of neuroblastoma diseases. in 85 per cent of the patients (n = 13) we stated an increased ejection of dopamine.	2006	2095056
diaphragmatic movement using ultrasound during spontaneous and mechanical ventilation: effect of tidal volume.	using ultrasound (us) the effect of various tidal volumes on the movement of ventral, dome and dorsal parts of the right hemidiaphragm was studied, both during spontaneous and mechanical ventilation. six healthy non-medicated volunteers who were in the supine position breathed spontaneously shallowly (tidal volume (vt) being 400 ml) (sb), and deeply (vt 1000 ml) (sb-deep). in addition, they were mechanically ventilated with intermittent positive pressure ventilation at three different vt's: 500 ...	2006	1449048
evaluation of the child with idiopathic mental retardation.	a review of the advances in diagnostic techniques for evaluation of children with idiopathic mental retardation is presented. the current status of the use of clinical genetics, cytogenetics, molecular genetics, and neuroimaging in evaluating children with mental retardation is emphasized. special attention is given to the evaluation of children with mental retardation and "autism" or "cerebral palsy."	2006	1635812
a review of the safety profile of teicoplanin.	safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ...	2006	1829079
a review of the safety profile of teicoplanin.	safety of teicoplanin has been assessed in 3377 patients treated in europe up to the end of june 1990. one or more adverse events were experienced by 10% of patients. age and teicoplanin dose had no significant effect on the incidence or type of adverse event. in comparative trials the incidence and profile of adverse events to teicoplanin have been similar to those seen with beta-lactam therapy. impaired renal function occurred consistently more frequently with vancomycin therapy than with teic ...	2006	1829079
health checks on patients 75 years and over in nottinghamshire after the new gp contract.	to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.	2006	1393076
[bilateral claw deformity of the fingers due to fasciitis with eosinophilia (shulman's syndrome). apropos of a case].	shulman's syndrome or fasciitis with eosinophilia is a disease of unknown origin with unclear relations with scleroderma, characterised by thickening of the fascia superficialis associated with blood eosinophilia. this diagnosis should be considered in a patient presenting with a lesion of the anterior surface of the forearms, which have an "armoured" appearance, associated with claw deformity of the wrist, hand and fingers. the diagnosis is based on cutaneo-muscular biopsy and treatment consist ...	2006	1698089
the use of potassium-titanyl-phosphate laser for laparoscopic removal of ovarian endometrioma.	from january 1989 through january 1990, 31 patients thought to have ovarian endometrioma at ultrasonography were treated by operative laparoscopy and potassium-titanyl-phosphate laser. the endometriomas ranged from 2 to 9.2 cm in diameter by preoperative transvaginal ultrasonography. the operative procedure used was a three-puncture technique with a 600 micron fiber attached to a potassium-titanyl-phosphate laser. the power setting was 10 w with a continuous pulse. in all cases endometriomas wer ...	2006	1828651
dual-ended, multifunction tools for microsurgery.	a new concept in microsurgical instrumentation is herein described and the rationale for its design presented in the context of surgical efficiency. this new concept employs bipolarity or dual-endedness as a means to enable the microsurgeon to have access to various instruments without the need to exchange tools. the elimination of instrument exchange has increased the speed with which a microsurgeon can perform a variety of procedures as well as decrease fatigue associated with continuous visua ...	2006	4088033
iron status markers in hereditary haemochromatosis: distinction between individuals being homozygous and heterozygous for the haemochromatosis allele.	iron status markers, serum iron, serum transferrin, transferrin saturation and serum ferritin were analysed in 162 homozygous patients with clinical haemochromatosis, in 12 homozygous relatives with preclinical haemochromatosis, in 84 heterozygous, and in 9 normal subjects. in the distinction between homozygous patients with clinical haemochromatosis and heterozygotes, transferrin saturation and serum ferritin showed the highest diagnostic efficiencies. a diagnostic efficiency of 0.97 was obtain ...	2007	1954989
[the topographic anatomical basis for ventriculosinusal shunting in hydrocephalus in nursing and young infants].	the peculiarities in the structure and topographic anatomical relations of the superior longitudinal, lateral, and sigmoid sinuses were studied on block preparations of the posterior cranial fossa of 14 children under 3 years of age who died from somatic diseases. it was shown that intrasinus inclusions preventing catheterization of the sinus (bands and septa) were absent at the junction of the first angle of the sigmoid sinus with its descending part. the authors recommend this area for shuntin ...	2007	2168650
[the effect of the application technic on the formal success of large-field radiotherapies].	198 patients suffering from hodgkin's disease or non-hodgkin's lymphomas were irradiated with large-field techniques from 1979 to 1988. 129 of these were treated with common block techniques whereas the field-integrated dose modification (fidm) was applied to 69 patients. retrospective analysis shows that fidm decreases acute toxicity. this was also evident in those patients who were irradiated by accelerated hyperfractionation. although single doses were higher in patients treated with fidm tre ...	2007	2000545
pain, prostaglandins, and food lipids. influences of dietary polyunsaturated fatty acids on eicosanoid synthesis and effects.		2007	2870709
[status of the venous part of the portal system in infectious-toxic shock in infants with acute pneumonia].	as many as 38 children aged 2 to 12 months suffering from acute pneumonia complicated by infectious-toxic shock manifested dilatation of the veins of portal circulation and deposition of a considerable blood volume, which was revealed by ultrasonic examination. the hemodynamic disorders were shown to play the leading part in the genesis of hepatomegaly, followed by hypoxic derangement of the hepatocyte. in infants, who are in a state of infectious-toxic shock, vascular decompensation of the live ...	2007	2075039
allergic contact cheilitis from toothpastes.		2007	1868726
lymphoid apoptosis in edwardsiella tarda septicemia in tilapia, oreochromis niloticus.	the present study revealed a relationship between the kinetic change of apoptosis and the inflammatory response during experimental intraperitoneal infection with edwardsiella tarda as a septicemic model. the morphological changes of apoptotic cells including cellular shrinkage, condensed nuclear chromatin, nuclear fragmentation and membrane blebbing were detected by light and transmission electron microscopy. tunel and agarose gel electrophoresis confirmed the fragmentation of dna in the apopto ...	2007	17046284
molecular cloning and characterization of toll-like receptor 9 in japanese flounder, paralichthys olivaceus.	toll-like receptor (tlr) 9 cdna and gene were cloned from japanese flounder, paralichthys olivaceus. the japanese flounder tlr9 cdna encodes 1065 amino acids. the leucine-rich domain (lrd) and the toll/interleukin-1 receptor (tir) domain found in other vertebrate tlr9s were conserved in japanese flounder tlr9. the gene is composed of three exons and two introns. the japanese flounder tumor necrosis factor (tnf) gene promoter was activated in japanese flounder tlr9-transformed hirame natural embr ...	2007	17118454
dissection of a type vi secretion system in edwardsiella tarda.	bacterial pathogens use different protein secretion systems to deliver virulence factors. recently, a novel secretion system was discovered in several gram-negative bacterial pathogens, and was designated as the type vi secretion system (t6ss). in edwardsiella tarda, a partial e. tardavirulent protein (evp) gene cluster was implicated in protein secretion. here, we identified the entire evp cluster as a t6ss and two additional secreted proteins (evpi, a homologue of vgrg, and evpp) were found. w ...	2007	17986187
effect of dietary supplementation of probiotic and vitamin c on the immune response of indian major carp, labeo rohita (ham.).	the immunostimulatory effect of probiotics and vitamin c has been established in many systems including fish. an investigation was carried out to study the effect of dietary supplementation of a probiotic bacterium "bacillus subtilis", vitamin c in the form of ascorbyl polyphosphate and their combination on the immune response of indian major carp, rohu, (labeo rohita ham.) fingerlings fed for a period of 60 days. the total serum protein and globulin content was significantly higher (p<0.05) in ...	2007	17434319
genotyping of edwardsiella tarda isolated from freshwater fish culture system.	the applicability of pcr-rflp of 16s rdna and conventional phenotypic methods for differentiation of edwardsiella tarda associated in freshwater fish culture system was studied. in this study, by conventional biochemical tests and antibiotic resistant patterns 2 and 14 groups were obtained. but these methods failed to discriminate the isolates habitat wise. however, pcr-rflp of 16s rdna was found to be specific to detect habitat-specific isolates. all the fish isolates belonging to particular ge ...	2007	17113645
probiotic properties of lactobacillus and bifidobacterium strains isolated from porcine gastrointestinal tract.	one strain of lactobacillus salivarius, two strains of lactobacillus reuteri and lactobacillus amylovorus, and two strains of bifidobacterium thermacidophilum with antagonistic effect against clostridium perfringens were isolated from porcine gastrointestinal tract. isolates were assayed for their ability to survive in synthetic gastric juice at ph 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract. there was a large variation in the survival of the iso ...	2007	17136367
gene expression of leucocytes in vaccinated japanese flounder (paralichthys olivaceus) during the course of experimental infection with edwardsiella tarda.	in this paper, we focused on the detection of differentially expressed genes in peripheral blood leucocytes (pbl) during the course of edwardsiella tarda infection in vaccinated and non-vaccinated japanese flounder (paralichthys olivaceus). cdna microarray analysis was performed to compare the gene expression patterns of the pbl between the vaccinated and non-vaccinated fish in response to e. tarda inoculation. fish were vaccinated twice, at a two-week interval and experimentally challenged with ...	2007	17049272
serum antibody response of indian major carp, labeo rohita to three species of pathogenic bacteria; aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens.	the immune response to mixed whole cell antigens of aeromonas hydrophila, edwardsiella tarda and pseudomonas fluorescens, the common gram negative bacterial pathogens associated with diseases of indian major carps were evaluated for their efficacy in triggering antibody responses in rohu, labeo rohita (ham.). the rohu yearlings were either immunized with antigens from single bacterial strain, a. hydrophila, e. tarda and p. fluorescens or a combination of all three. an antibody response was detec ...	2007	17383016