Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| differential expression of crown gall tumor markers in transformants obtained after in vitro agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from nicotiana tabacum. | to obtain transformation of plant cells, we incubated 3-day-old cell wall-regenerating protoplasts from tobacco with agrobacterium tumefaciens harboring tumor-inducing plasmids. putative transformed tobacco cells were selected by phytohormone autotrophic growth and were shown to be transformed by the detection of the tumor cell specific enzymes lysopine dehydrogenase or nopaline dehydrogenase. this was substantiated by the detection, in transformed tumor tissues, of dna sequences homologous to s ... | 1981 | 16593059 |
| crown-gall tumors possess tumor-specific antigenic sites on their cell walls. | rabbits were injected with cell walls obtained from crown-gall tumor tissue or the corresponding cell walls from normal potato tissue. the serum obtained from rabbits 53 days after they were injected with tumor cell walls contained immunoglobins that reacted with both tumor and normal cell walls as well as with the cells from the inciting strain of agrobacterium tumefaciens. when this serum was repeatedly absorbed against normal cell walls and the cells of the inciting strain of agrobacterium tu ... | 1981 | 16661835 |
| strain-dependent temperature-sensitive phase in crown gall tumorigenesis. | the effect of high temperature treatments on the early stages of crown gall tumorigenesis in sunflowers was investigated. treatments of 32 c initiated at various times during the first ten days after infection had a similar effect on tumors induced by agrobacterium tumefaciens strains b(6) and c58. tumor growth was sensitive to 32 c until 60 to 72 hours after infection and was stimulated by 32 c after that time. therefore, the "inception phase" for both c58 and b(6)-induced tumors ends between 6 ... | 1981 | 16661888 |
| phytohormones in the formation of crown gall tumors. | crown gall tumors were initiated in a variety of plant species by infection with agrobacterium tumefaciens strain b6 and the concomitant changes in the tissue levels of phytohormones, mainly indole-3-acetic acid (iaa) and cytokinins, were analyzed. a comparison was made of these hormones with those produced by virulent and avirulent strains of the bacterium in liquid culture and with those of bacteria-free crown gall callus cultures. specific radioimmunoassays were employed for hormone determina ... | 1981 | 24276937 |
| octopine accumulation early in crown gall development is progressive. | a purification of octopine from crown gall tissue was developed to quantitate conversion of precursor [(3)h]arginine into [(3)h]octopine. plant wound tissue which was sterile or infected with an avirulent strain of agrobacterium tumefaciens did not accumulate detectable quantities of octopine, consistent with opine synthesis not being induced by wounding or infection. octopine was only recovered from tissue infected with virulent tumor-inducing strains of a. tumefaciens. in every case tested, th ... | 1982 | 16662161 |
| characteristics of teratomas regenerated in vitro from octopine-type crown gall. | crown galls induced by infection of tobacco plants with agrobacterium tumefaciens strain c58-cl(ptib6s3) were excised and cultured in vitro. after about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. leaf explants from shoots of tumor-line t5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. detached leaves from t5 shoots also synthesized octopine. these result ... | 1982 | 16662180 |
| octopine synthase mrna isolated from sunflower crown gall callus is homologous to the ti plasmid of agrobacterium tumefaciens. | we have shown that the structural gene for octopine synthase (a crown gall-specific enzyme) is located in a central portion of the t-dna that came from the ti plasmid of agrobacterium tumefaciens and is expressed after it has been transferred to the plant cells. polyadenylylated rna was prepared from polysomes isolated from an octopine-producing crown gall callus and purified by selective hybridization to one of five recombinant plasmids. each such plasmid contained a different fragment of t-dna ... | 1982 | 16593142 |
| agrobacterium ti plasmid indoleacetic acid gene is required for crown gall oncogenesis. | a gene (iaap) necessary for virulence and indoleacetic acid (iaa) production has been located on a nopaline ti plasmid of agrobacterium tumefaciens c58. iaap function was established by using transformation to insert nopaline or octopine ti plasmids into an avirulent, ti plasmid-free mutant 1d1293-3 that was defective in iaa synthesis (iaac(-)). the resulting transformants produced increased levels of iaa and virulence was restored. when these transformants were cured of their ti plasmid, virule ... | 1982 | 16593184 |
| psi, a temperate phage of agrobacterium tumefaciens, is mutagenic. | a new temperate bacteriophage (phage psi) of agrobacterium tumefaciens strain b91 is described. many octopine-utilizing strains of a. tumefaciens harbor prophage psi or a phage that is similar if not identical to it. this phage has a very narrow host range, and we found that its growth is strongly reduced in strains which carry an octopine pti plasmid. when sensitive bacteria are infected with psi, 1 to 3% of the survivors carry mutations on the chromosome, as well as on the pti plasmid. this ph ... | 1982 | 16789221 |
| metal coordination centres of class ii cytochromes c. | the class ii cytochromes rhodospirillum molischianum cytochrome c', rhodopseudomonas palustris cytochrome c556 and agrobacterium tumefaciens (b2a) cytochrome c556 have been investigated with a variety of spectroscopic techniques. the cytochrome c' was found to be high-spin and the two cytochromes c556 were found to be mainly low-spin and sx-coordinate with the fifth and sixth ligands being histidine and methionine. the implications of the different types of iron coordination are discussed. | 1982 | 6279397 |
| construction and application of r prime plasmids, carrying different segments of an octopine ti plasmid from agrobacterium tumefaciens, for complementation of vir genes. | 1982 | 6281831 | |
| octopine ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the t-region. | 1982 | 6283573 | |
| genetic map of the crown gall suppressive incw plasmid psa. | a genetic map of the w incompatibility group plasmid psa has been prepared through the construction of deletion derivatives of psa and the cloning of various fragments of psa in pbr322. phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol, sulfonamides, spectinomycin, streptomycin, kanamycin, gentamycin, and tobramycin. information sufficient for the replication of the plasmid in both escherichia coli and agrobacterium tumefaciens i ... | 1982 | 6287164 |
| t-dna organization in homogeneous and heterogeneous octopine-type crown gall tissues of nicotiana tabacum. | octopine-type tumor tissue was obtained both by infection of plants or isolated protoplasts with agrobacterium tumefaciens and by somatic hybridization of normal and crown gall tobacco cells. analysis of t-dna by southern blotting of clones and uncloned tissue reveals that, whereas tumors induced on plants are heterogeneous mixtures of cells differing in t-dna organization, each tissue derived from transformed protoplasts or from somatic hybridization is homogeneous. detailed analysis of t-dna o ... | 1982 | 6291777 |
| agrobacterium tumefaciens mutants affected in attachment to plant cells. | an analysis of agrobacterium tumefaciens mutants with tn5 insertions in chromosomal dna showed that the chromosome of a. tumefaciens codes for a specific ability of this bacterium to attach to plant cells. this ability is associated with tumorigenesis by a. tumefaciens, the ability of avirulent a. tumefaciens to inhibit tumorigenesis, and the ability to adsorb certain phages. a second class of chromosomal mutations affects tumorigenesis without altering the ability to attach to plant cells. the ... | 1982 | 6292165 |
| units of genetic expression in the virulence region of a plant tumor-inducing plasmid of agrobacterium tumefaciens. | the effect of a large number of tn3 insertions in the vir region of the ti plasmid ptia6nc on the virulence of agrobacterium was determined. the vir- insertions were mapped in three of the five loci that have been defined previously. merodiploid rec- strains carrying one insertion mutation on the ti plasmid and another insertion mutation (or the homologous wild-type region) on a compatible plasmid were constructed and used in complementation tests for virulence in test plants. this analysis has ... | 1982 | 6298572 |
| t-dna of pti-15955 from agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated rnas in a sunflower crown gall tumor. | northern blot hybridization analysis of polysomal polyadenylated rna isolated from sunflower crown gall tumor pscg-15955 demonstrated that a minimum of seven rnas were transcribed from t-dna of pti-15955 from agrobacterium tumefaciens. the sizes of the t-dna transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. the relative abundance of these polyadenylated rnas varied greatly, the 1.0 kb rna being the most abundant and the 1.6 kb rna being the least abunda ... | 1982 | 6175956 |
| biochemical studies on cytoplasmic membranes of agrocin sensitive and resistant strains of agrobacterium tumefaciens. | 1982 | 6184960 | |
| studies on superoxide dismutase activities in virulent and avirulent strains of agrobacterium tumefaciens and also in normal and crown gall tumor cells of bryophyllum calycinum. | superoxide dismutase activity in virulent strains of agrobacterium tumefaciens was found to be higher than that in avirulent strains. polyacrylamide gel electrophoresis revealed two isoenzymes in both these strains. these isoenzymes are suggested to be iron and manganese containing superoxide dismutases. crown gall tumor cells of the plant bryophyllum calycinum were found to have higher superoxide dismutase activity than the normal plant cells. polyacrylamide gel electrophoresis revealed two iso ... | 1982 | 6188336 |
| partial purification and properties of membrane-bound atpase in agrobacterium tumefaciens. | 1982 | 6220646 | |
| variation in hormone autonomy and regenerative potential of cells transformed by strain a66 of agrobacterium tumefaciens. | mutant agrobacterium tumefaciens strain a66 is shown to differ from its wild-type progenitor (strain a6) by a spontaneous 2.7 kb dna insert into the t-dna region of its ti plasmid. tobacco stems transformed by a66 exhibit an attenuated response characterized by slow growth and shoot proliferation. clonal analysis demonstrates that this response is due to an alteration in the growth and regenerative potential of transformed cells, rather than to variation in the frequency of fully autonomous cell ... | 1982 | 6297775 |
| ferrisiderophore reductase activity in agrobacterium tumefaciens. | reduction of the iron in ferriagrobactin by the cytoplasmic fraction of agrobacterium tumefaciens strictly required nadh as the reductant. addition of flavin mononucleotide and anaerobic conditions were necessary for the reaction; when added with flavin mononucleotide, magnesium was stimulatory. this ferrisiderophore reductase activity may be a part of the iron assimilation process in a. tumefaciens. | 1982 | 7056702 |
| relationship between nif plasmids of fast-growing rhizobium species and ti plasmids of agrobacterium tumefaciens. | by use of the southern blot hybridization technique the extent of dna homology was determined between the nif plasmid of a number of fast-growing rhizobium species and ti plasmids of the octopine (ptiach5) and nopaline (ptic58) type. dna sequences common to these plasmids were located on functional maps of the ti plasmids. no homology between nif plasmids and the t region of ti plasmids was detected. | 1982 | 7061382 |
| complementation analysis of agrobacterium tumefaciens ti plasmid mutations affecting oncogenicity. | a wide host range cosmid vector has been constructed by insertion of the lambda cos site into the plasmid prk2501. this cosmid, which is maintained in agrobacterium tumefaciens and is compatible with the ti plasmid, has been used to make a clone bank of the a. tumefaciens ptia6 plasmid. several ptia6 cosmids have been used to complement tn5-induced ti plasmid mutations. five avirulent mutations which map outside of the region of the plasmid maintained in plant tumours (t-dna) could be complement ... | 1982 | 7061395 |
| d-glucosaminate dehydratase from agrobacterium radiobacter. physicochemical and enzymological properties. | the bacterial distribution of d-glucosaminate dehydratase [ec 4.2.1.26] was investigated and agrobacterium radiobacter (iam 1526) was found to have the highest enzyme activity. the enzyme was formed inducibly in a glycerol-urea medium by d-glucosamine, d-galactosamine, and d-glucosamine, but not by d-mannosamine. the enzyme purified from the cells grown in the glucosamine-glycerol-urea medium was shown to be homogeneous by ultracentrifugation. the molecular weight was determined to be about 66,0 ... | 1982 | 7068563 |
| [physiological and biochemical characteristics of the cells of a carrot gall tumor developing in weightlessness]. | the paper presents the results of experiments with the carrot tissues infected with agrobacterium tumefaciens flown onboard the biosatellite cosmos-1129 in cooperation with the us scientists. postflight, the respiratory activity of tumour cells was determined and k+ and na+ permeability of cell membranes was measured. the resulting data give evidence that in weightlessness the development of the carrot gall tumour is accompanied by changes in the above physiological and biochemical parameters. t ... | 1982 | 7070038 |
| secretion of trans-zeatin by agrobacterium tumefaciens: a function determined by the nopaline ti plasmid. | 1982 | 7073755 | |
| em5400, a family of monobactam antibiotics produced by agrobacterium radiobacter. ii. isolation and structure determination. | 1982 | 7076578 | |
| characteristics of ti plasmids from broad-host-range and ecologically specific biotype 2 and 3 strains of agrobacterium tumefaciens. | agrobacterium tumefaciens strains isolated from crown gall tumors on grapevines in california were consistently of the biotype 3 group. all 11 of these strains were limited in their host range and harbored ti plasmids with molecular masses between 119 and 142 megadaltons (mdal) as well as a larger cryptic plasmid of greater than 200 mdal; occasionally a smaller cryptic plasmid of 65 mdal was also present. ti plasmids o these strains have dna sequences in common with ti plasmids of octopine and n ... | 1982 | 7085561 |
| regions of homology between tumorigenic plasmids from agrobacterium rhizogenes and agrobacterium tumefaciens. | 1982 | 7089090 | |
| tumor induction by agrobacterium tumefaciens: analysis of the boundaries of t-dna. | molecular cloning has been used to isolate the ends of that portion of the tumor-inducing (ti) plasmid of agrobacterium tumefaciens which has been designated t-dna and which has been transferred to the genome of tobacco crown gall tumor cells. analysis of the dna sequences of the plant border clones compared with the corresponding sequences of the ti plasmid suggests that the mechanism of transferred dna integration and subsequent stabilization is precise at the right border and imprecise on the ... | 1982 | 7108407 |
| passage of bacterial dna into host cells during in vitro transformation of nicotiana tabacum by agrobacterium tumefaciens. | tumorigenic and non-tumorigenic strains of agrobacterium tumefaciens were labelled with tritiated thymidine and added separately to suspension cultures of x-d line habituated tobacco cells. the subsequent association of bacteria with plant host cells and the passage of bacteria and bacterial dna into these cells was examined by conventional light and electron microscopy and light microscope autoradiography. the results suggest that both strains of bacterium enter damaged host cells (via wall les ... | 1982 | 7144594 |
| identification of the sym plasmid of rhizobium leguminosarum strain 1001 and its transfer to and expression in other rhizobia and agrobacterium tumefaciens. | 1982 | 7146147 | |
| dna from the a6s/2 crown gall tumor contains scrambled ti-plasmid sequences near its junctions with plant dna. | the a6s/2 tumor incited on tobacco by agrobacterium tumefaciens harboring the octopine-type a6 ti plasmid contains one insert of ti-plasmid sequences (the t dna). this 13 kb insert is derived from a colinear sequence in the ti plasmid (the t region) and becomes attached to plant dna in the nucleus of the host cell. we have determined the dna sequence encompassing the left end of the t region of the a6 ti plasmid and the corresponding portion of the a6s/2 t dna. the two sequences are identical fo ... | 1982 | 7151163 |
| nucleotide sequence and transcript map of the agrobacterium tumefaciens ti plasmid-encoded octopine synthase gene. | we have determined the complete nucleotide sequence of the gene for the crown gall enzyme, octopine synthase. the sequence was derived from cloned fragments of the agrobacterium tumefaciens ti plasmid ach5. it displayed a continuous open reading frame encoding a polypeptide chain of 358 amino acids. the nucleotide positions corresponding to the 5' end and poly(a) addition site of the mature octopine synthase mrna from a tobacco tumor cell line were determined by s1 nuclease mapping. two sequence ... | 1982 | 7153687 |
| nopaline synthase: transcript mapping and dna sequence. | the dna sequence of the nopaline synthase gene (nos) from agrobacterium tumefaciens ti plasmid ptit37 and adjacent regions up to the right border of the t-dna was determined. the 5' and 3' termini of the polyadenylated nos mrna, isolated from a t37 tobacco teratoma tumor line, were localized by s1 mapping. the final mrna is unspliced, encoded by a region of about 1450 bp, and specifies an open reading frame of 413 amino acids. potential transcriptional signals in the 5' flanking dna, such as cat ... | 1982 | 7153689 |
| modification and evaluation of the potato disc assay and antitumor screening of euphorbiaceae seeds. | galsky et al. (10, 11) have reported that the inhibition and growth of crown gall tumors, initiated on potato discs by agrobacterium tumefaciens, apparently has good agreement with 3ps in vivo antileukemic activity in mice. we have now modified and evaluated this assay for its potential use as a prescreen and fractionation monitor of plant extracts for 3ps activity. the modified assay was performed on a series of natural compounds and plant extracts (known to have 3ps activity) and on extracts o ... | 1982 | 7161602 |
| occurrence of l-lysine epsilon-dehydrogenase in agrobacterium tumefaciens. | a novel amino acid dehydrogenase catalyzing the oxidative deamination of the epsilon-amino group of l-lysine was found in the crude extract of agrobacterium tumefaciens icr 1660. the enzyme required nad(+) and was specific for l-lysine. the enzyme was optimally active at about ph 9.7. | 1982 | 6801024 |
| biosynthesis of monobactam compounds: origin of the carbon atoms in the beta-lactam ring. | the biosynthesis of monobactams by strains of chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter was studied. monobactams were produced during logarithmic growth by c. violaceum and acetobacter sp. and during late log growth on glycerol and in stationary phase by a. radiobacter. the addition of various amino acids failed to significantly stimulate monobactam production in any of the producing organisms. several 14c-amino acids and pyruvate were incorporated in vivo into mo ... | 1982 | 6805424 |
| r68.45 mediated chromosomal gene transfer in agrobacterium tumefaciens. | a large plasmid enables its host agrobacterium tumefaciens to cause tumorous condition in a wide variety of dicotyledonous plants[see ooms et al. gene 14:33--50 (1981) )). the location and role of chromosomal genes in this phenomenon are not known. as the first stage in studying this aspect, a project was initiated to investigate the chromosomal genetics of the bacterium. r68.45, a p group plasmid, was chosen as a transmission agent. after a preliminary assessment it was decided to use c58 as a ... | 1982 | 6808964 |
| lytic effects of edta & lysozyme on virulent & avirulent strains of agrobacterium tumefaciens. | 1982 | 6809600 | |
| fate in model ecosystems of microbial species of potential use in genetic engineering. | the changes in populations of staphylococcus aureus, bacillus subtilis, salmonella typhimurium, klebsiella pneumoniae, agrobacterium tumefaciens, rhizobium meliloti, and saccharomyces cerevisiae were measured after their introduction into samples of sewage, lake water, and soil. enumeration of small populations was possible because the strains used were resistant to antibiotics in concentrations and combinations such that few species native to these ecosystems were able to grow on agar containin ... | 1982 | 6814361 |
| indoleacetic acid complementation and its relation to host range specifying genes on the ti plasmid of agrobacterium tumefaciens. | host range variations were noted when 23 wild-type strains of agrobacterium tumefaciens were tested on 27 different plant species. because we have shown previously that host range specificity is conferred by the pti plasmid, these variations in host specificity implicated genetic differences among pti plasmids within the a. tumefaciens population that was tested. host specificity was independent of the type of opine utilized and biotype of the strain used. these data suggested that separate gene ... | 1982 | 6962315 |
| em5400, a family of monobactam antibiotics produced by agrobacterium radiobacter. i. taxonomy, fermentation and biological properties. | 1982 | 6978878 | |
| antimicrobial, insect sterilizing and ovicidal activity of some oxo-vanadium(iv) and oxo-vanadium(v) complexes. | twenty-three newly synthesized mixed-ligand complexes of oxo-vanadium(iv) and oxo-vanadium(v) were studied for antimicrobial activity. eight of these complexes were found to have microbicidal properties. the complexes [nh4][vo(gl)2]h2o (gl-h2 = glycolic acid) and [vo(acoap)(acac-h)]h20 (acoap-h2=schiff base of acetylacetone and orthoaminophenol, acac-h=acetyl-acetone) show broad bactericidal spectra, while the complexes [vo(acsam)2]oh (acsam-h = schiff base of acetylacetone and sulphanilamide) ... | 1982 | 6983826 |
| crown gall disease and prospects for genetic manipulation of plants. | agrobacterium tumefaciens incites crown gall tumors when bacterial dna integrates into plant nuclear dna. plant cells can express these integrated bacterial genes. following insertion of desired genes into bacterial dna using recombinant dna techniques, this system permits introduction of these new genes into plant dna. we discuss the potential for genetic manipulation of plants using agrobacterium tumefaciens and the related organism agrobacterium rhizogenes. | 1982 | 17807126 |
| the state of phage psi dna in lysogenic cells of agrobacterium tumefaciens. | phage psi a temperate phage of agrobacterium tunefaciens is mutagenic, grows poorly in strains which contain a pti plasmid, and can be transferred by conjugation (d. expert and j. tourneur, 1982, j. virol., 42, 283-291). in an attempt to explain these unusual properties we undertook a study of the state of psi dna in the virion, during the lytic cycle, and in lysogenic cells. in the virions, the phage genome was found to consist of circularly permuted molecules. covalently closed circular forms ... | 1982 | 18638749 |
| studies on the structure of cointegrates between octopine and nopaline ti-plasmids and their tumour-inducing properties. | stable cointegrates between incrh-1 octopine (ach5) and nopaline (c58) ti-plasmids, present in ten independently isolated agrobacterium tumefaciens strains, showed identical restriction endonuclease patterns. each cointegration event had taken place in the common sequence between the t-regions of both ti-plasmids. this illustrates a high preference for this region when used in the formation of cointegrates. four crown gall tissues, obtained after transformation of nicotiana tabacum cells by one ... | 1982 | 24318033 |
| influence of a crown-gall tumor initiation enhancer on bacterial attachment to the host plant cell wall. | the competitive activities of different plant cell walls upon agrobacterium tumefaciens attachment have been studied in vitro by means of two crown-gall tumor initiation assays. the low or high susceptibility of different plant species is independent of their capacity to cause bacterial cells to adhere to specific sites on the plant cell walls. however, the attachment properties of cell wall fragments derived from helianthus cotyledons seem to be age-dependent. it is found that a tumor initiatio ... | 1982 | 24272582 |
| evidence for the existence of a universal crown-gall tumor initiation enhancer. | tumor initiation of different dicotyledonous plant species inoculated with agrobacterium tumefaciens b6 has been studied in vivo and in vitro. the tumor formation in weakly susceptible plants can be strongly enhanced by exogenously applied active extract fractions derived from highly susceptible helianthus cotyledons. it is found that highly susceptible plants (kalanchoë, lycopersicon and pinto beans) contain an active tumor initiation enhancer which is clearly similar to the compound(s) found i ... | 1982 | 24272581 |
| double infection of tobacco plants by two complementing octopine t-region mutants of agrobacterium tumefaciens. | the molecular basis of complementation by a mixture of two different types of octopine t-region mutants (lba4060 and lba4210) was studied. six randomly chosen cellular clones derived from a tumor obtained after mixed infection were analyzed for their t-dna content via southern blot hybridization. the clones appeared to contain t-dna that originated from each of both mutants, indicating that they developed from doubly infected single cells. genetic complementation, therefore, might explain at lea ... | 1982 | 24317964 |
| the lysopinedehydrogenase gene used as a marker for the selection of octopine crown gall cells. | plant cells transformed into octopine-synthesizing tumour cells by the bacterium agrobacterium tumefaciens survive when cultured in the presence of homo-arginine (ha), whereas both normal plant cells and nopaline producing plant tumour cells do not. survival of octopine crown gall cells is due to the activity of the enzyme lysopinedehydrogenase (lpdh) in these cells, which converts toxic homo-arginine into non-toxic homo-octopine. the selective toxicity of homo-arginine for normal cells can be a ... | 1982 | 24317894 |
| introduction of genetic material into plant cells. | the tumor-inducing (ti) plasmid of the soil microorganism agrobacterium tumefaciens is the agent of crown gall disease in dicotyledonous plants. the ti plasmid contains two regions that are essential for the production of transformed cells. one of these regions, termed transfer dna, induces tumor formation and is found in all established plant tumor lines; the other, termed the virulence region, is essential for the formation but not the maintenance of tumors. transfer dna, which transfers to th ... | 1983 | 17738341 |
| genetic analysis of t-dna transcripts in nopaline crown galls. | plant crown gall tumor cells result from the insertion and expression of a defined dna sequence, called t-dna, which is derived from the ti plasmid, harbored by agrobacterium tumefaciens strains. to study the function of the genes of the t-dna of the nopaline ti plasmid, ptic58, a collection of mutants was isolated so that t-dna genes are inactivated either separately or in various combinations. it was found that no single t-dna gene or t-region border is absolutely essential for stable tumor fo ... | 1983 | 6839358 |
| sulfur metabolism in the biosynthesis of monobactams. | we studied the biosynthesis of monobactams with respect to sulfur metabolism in chromobacterium violaceum, acetobacter sp., and agrobacterium radiobacter. all three organisms used inorganic sulfur for monobactam production. when sulfur-containing amino acids were assayed as a source of sulfur for monobactam production, c. violaceum used cystine but not cysteine or methionine, acetobacter sp. used all three compounds, and a. radiobacter used none. 35s from cysteine, methionine, and sodium sulfate ... | 1983 | 6859838 |
| morphology of root nodules and nodule-like structures formed by rhizobium and agrobacterium strains containing a rhizobium meliloti megaplasmid. | we examined expression of the megaplasmid prme41b of rhizobium meliloti in two different rhizobium sp. strains and in agrobacterium tumefaciens. transfer of prme41b into these bacteria was facilitated by insertion of a recombinant plasmid coding for mobilization functions of rp4 into the nif region (kondorosi, a., e. kondorosi, c.e. pankhurst, w. j. broughton, and z. banfalvi, 1982, mol. gen. genet., 188:433-439). in all cases, transconjugants formed nodule-like structures on the roots of medica ... | 1983 | 6885919 |
| a functional map of the nopaline catabolism genes on the ti plasmid of agrobacterium tumefaciens c58. | the nopaline catabolism (noc) genes are located in a 14.4 kb region on the ptic58 plasmid of a. tumefaciens c58. these genes permit the bacterium to grow on nopaline n2-(1,3-dicarboxylpropyl) arginine, a substrate produced in plant tumors initiated by strain c58. the functions of the noc genes include the use of nopaline and l-ornithine as sole carbon and nitrogen sources. using tn5 insertional mutants, we have identified and mapped the positions of the genes that are responsible for nopaline ca ... | 1983 | 6577260 |
| a general method for the transfer of cloned genes to plant cells. | this paper describes a method for the transfer to plant cells of any cloned gene, regardless of its termini or internal restriction enzyme cleavage sites. a broad host-range intermediate vector, pgv1117, was constructed containing hindiii-23, a right-end t-region fragment of the nopaline plasmid ptic58. using in vivo protection by ecori methylase and ecori linker ligation, a fragment of rabbit chromosomal dna, carrying the beta-globin gene, was inserted into plasmid pgv1117. following transmissi ... | 1983 | 6628995 |
| presence of a new cytochrome b - like pigment with a peak at 567 nm in various aerobic bacteria. | several physiological groups of bacteria were examined for the presence of a cytochrome b - like pigment which is demonstrable in dithionite-reduced minus substrate-reduced difference spectra. this pigment is characterized by an unusually high alpha band at 567 nm, a low concentration relative to conventional cytochromes, and an inability to be fully reduced by endogenous substrates or nadh. previous studies with one denitrifying and nondenitrifying species of the genus pseudomonas, in paracoccu ... | 1983 | 6652580 |
| relationship between adenylate cytokinin production and ti plasmid of agrobacterium tumefaciens. | to know whether the tumor-inducing plasmid of agrobacterium tumefaciens carries genetic information of the biosynthesis of cytokinins, the levels of 6-(3-methyl-2-butenyl-amino)purine (ipade) and its 4-hydroxy derivative trans-zeatin (trans-z) and its p-beta-d-ribofuranoside (trans-zr) produced in media by wild-type virulent strain, plasmid-cured avirulent strain and the deletion mutant were compared. the highest levels of ipade and trans-z were found in the culture filtrate of late-log phase gr ... | 1983 | 6664842 |
| physical & chemical studies on lipopolysaccharide of agrobacterium tumefaciens. | 1983 | 6671675 | |
| the complete amino-acid sequence of the low-spin class ii cytochrome c-556 from agrobacterium tumefaciens strain b2a. | the amino acid sequence of the soluble monohaem cytochrome c-556 from agrobacterium tumefaciens, strain b2a, has been determined. the sequence was derived from peptides obtained by digestion of the apoprotein with trypsin and chymotrypsin, and by subdigestion of some of the peptides with staphylococcus aureus protease and thermolysin. sequencing of the various peptides was achieved by a combination of manual dansyl-edman degradation and automatic liquid-phase sequence analysis. the main characte ... | 1983 | 6297889 |
| transposon-facilitated chromosome mobilization in agrobacterium tumefaciens. | we improved chromosomal gene transfer in agrobacterium tumefaciens strain 15955 by constructing donors containing homologous transposons on both the sex factor plasmid and chromosome. first, we constructed plasmid pdp35, a kanamycin-sensitive derivative of r68.45. we then constructed derivatives of pdp35 that contained insertions of the kanamycin resistance transposon tn5. by restriction endonuclease analysis, we identified two plasmids, pdp37 and pdp38, in which tn5 was inserted in the same reg ... | 1983 | 6298186 |
| comparison of ti plasmids from three different biotypes of agrobacterium tumefaciens isolated from grapevines. | twenty-six plasmids from grapevine isolates of agrobacterium tumefaciens were analyzed by smai fingerprinting and by hybridization of nick-translated dna to dna of another plasmid. these experiments established that octopine ti plasmids are not highly conserved, although octopine ti plasmids from biotype 1 a. tumefaciens strains appeared to be very similar. octopine ti plasmids from biotype 3 strains are more variable in terms of host range and smai fingerprints, but share extensive dna homology ... | 1983 | 6298189 |
| [cloning of the ti-plasmid dna fragments of agrobacterium tumefaciens in escherichia coli and the identification of the site of origin of ti-plasmid replication]. | 1983 | 6321123 | |
| the role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor. | the ft37/1 tumor line induced by agrobacterium tumefaciens on flax epicotyls contains 22-24 copies of the t-dna encoded nopaline synthase gene per cell. all the gene copies are methylated to some extent but the methylation is not uniform, nor does it reflect the methylation level of the flanking plant dna. this extensive methylation correlates with an extremely low level of expression of the nopaline synthase gene. treatment of the tumor line with the in vivo demethylating drug 5-azacytidine at ... | 1983 | 6198416 |
| structure and transcription of the nopaline synthase gene region of t-dna. | we present the dna sequence and plant-tumor transcription pattern of some 2400 base pairs from the right border region of pti t37 dna from the virulent agrobacterium tumefaciens strain t37. this region includes the entire transcription unit encompassing the nopaline synthase gene, together with parts of other transcription units. the strategy used to determine the sequence also produced two opposing series of defined, asymmetric deletions across the target dna region, some of which may serve fut ... | 1983 | 6298724 |
| multiple mutations in the t region of the agrobacterium tumefaciens tumor-inducing plasmid. | three genetic loci affecting tumor morphology lie within ptia6nc t-dna: tms, tmr, and tml. using deletions and multiple transposon insertions, we constructed tumor-inducing (ti) plasmids representing every possible double and triple mutant combination. tms tmr and tms tmr tml mutants did not incite tumors on most plants and produced a very weak response on a few other hosts but tms tml and tmr tml mutants were virulent. thus, either tms+ or tmr+ alone can promote significant tumor growth but tml ... | 1983 | 6300864 |
| regeneration of intact tobacco plants containing full length copies of genetically engineered t-dna, and transmission of t-dna to r1 progeny. | cloned dna sequences encoding yeast alcohol dehydrogenase and a bacterial neomycin phosphotransferase have been inserted into the t-dna of agrobacterium tumefaciens plasmid ptit37 at the "rooty" locus. transformation of tobacco stem segments with the engineered bacterial strains produced attenuated crown gall tumors that were capable of regeneration into intact, normal tobacco plants. the yeast gene and entire transferred dna (t-dna) were present in the regenerated plants in multiple copies, and ... | 1983 | 6301678 |
| site-directed mutagenesis in escherichia coli of a stable r772::ti cointegrate plasmid from agrobacterium tumefaciens. | the host range of an octopine ti plasmid is limited to rhizobiaceae. this has been extended also to escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid r772. its structure was studied by constructing a physical map of r772 and of the r772::ptib6 cointegrate. an insertion sequence present in r772, called is70, turned out to be involved in cointegrate formation. we found one intact copy of is70 and a small segment of is70, respectively, at the junctions of r772 an ... | 1983 | 6302080 |
| role of bacterial cellulose fibrils in agrobacterium tumefaciens infection. | during the attachment of agrobacterium tumefaciens to carrot tissue culture cells, the bacteria synthesize cellulose fibrils. we examined the role of these cellulose fibrils in the attachment process by determining the properties of bacterial mutants unable to synthesize cellulose. such cellulose-minus bacteria attached to the carrot cell surface, but, in contrast to the parent strain, with which larger clusters of bacteria were seen on the plant cell, cellulose-minus mutant bacteria were attach ... | 1983 | 6302086 |
| ti plasmid and chromosomal ornithine catabolism genes of agrobacterium tumefaciens c58. | the ptic58 plasmid noc genes of agrobacterium tumefaciens c58 code for nopaline oxidase (nocc), nopaline permease (nocp), the inducible periplasmic protein n1 (nocb), and a function(s) required for ornithine catabolism (noca). in addition, strains c58 and ach-5 of a. tumefaciens have chromosomal ornithine catabolism genes. the chromosomal orc gene codes for ornithine dehydrogenase. strain c58 is normally orc, but orc+ mutants can be selected. we have characterized both chromosomal orc and ptic58 ... | 1983 | 6305908 |
| methylation of the t-dna in agrobacterium tumefaciens and in several crown gall tumors. | methylation of the t-dna in agrobacterium tumefaciens and in four octopine-type (a6s/2, e9, 15955/1, 15955/01) and one nopaline-type (ht37#15) crown gall tumors was investigated using the isoschizomeric restriction endonucleases msp i and hpa ii. t-dna in the octopine-type ti-plasmid ptib6(806) was not methylated at the sequence 5'ccgg3' in agrobacterium. with two possible exceptions, neither was the t-dna of the nopaline-type ti-plasmid ptit37 methylated in the bacterium. in all tumor lines inv ... | 1983 | 6306562 |
| nopaline ti-plasmid, ptit37, t-dna insertions into a flax genome. | the ft37/1 plant tumor line induced on flax epicotyls by t37, a nopaline strain of agrobacterium tumefaciens, contains multiple copies of the ptit37, t-dna. there are three to four distinct full-length insertions and one tandem insertion. allowing for the different copy numbers of the inserts, this amounts to seven to eight t-dna copies per genome unit. the genome unit in this case is the haploid dna value (7 x 10(8) bp) which predicts a t-dna copy number of 14-16 per diploid cell. three novel t ... | 1983 | 6308120 |
| expression of bacterial genes in plant cells. | chimeric bacterial genes conferring resistance to aminoglycoside antibiotics have been inserted into the agrobacterium tumefaciens tumor-inducing (ti) plasmid and introduced into plant cells by in vitro transformation techniques. the chimeric genes contain the nopaline synthase 5' and 3' regulatory regions joined to the genes for neomycin phosphotransferase type i or type ii. the chimeric genes were cloned into an intermediate vector, pmon120, and inserted into ptib6s3 by recombination and then ... | 1983 | 6308651 |
| the amino acid sequence of cytochrome c-556 from agrobacterium tumefaciens strain apple 185. | the evidence for the amino acid sequence of cytochrome c-556 from agrobacterium tumefaciens strain apple 185 is reported. the sequence was determined by manual edman degradation of tryptic and chymotryptic peptides using the dabitc/pitc double-coupling method; some peptides were further cleaved by partial acid hydrolysis and with staphylococcus aureus protease. the sequence overlaps 13-15, 83-85 and 106-108 as well as the region 113-118 involving the haem-binding sequence cys-xaa-xaa-cys-his wer ... | 1983 | 6309523 |
| nucleotide sequence of the agrobacterium tumefaciens octopine ti plasmid-encoded tmr gene. | the nucleotide sequence of the tmr gene, encoded by the octopine ti plasmid from agrobacterium tumefaciens (ptiach5), was determined. the t-dna, which encompasses this gene, is involved in tumor formation and maintenance, and probably mediates the cytokinin-independent growth of transformed plant cells. the nucleotide sequence of the tmr gene displays a continuous open reading frame specifying a polypeptide chain of 240 amino acids. the 5'- terminus of the polyadenylated tmr mrna isolated from o ... | 1983 | 6312414 |
| a new technique for genetic engineering of agrobacterium ti plasmid. | a new technique is described that allows easy introduction of foreign genetic elements into specific regions of agrobacterium tumefaciens dna. it uses plasmids that (1) can be introduced, but not maintained in a. tumefaciens, (2) have a region homologous to the genome of the recipient, and (3) have an appropriate marker. selection for the marker will yield transconjugants in which the introduced plasmid has recombined with the host genome. applications of the technique are described. | 1983 | 6312475 |
| transfer of an indigenous plasmid of rhizobium loti to other rhizobia and agrobacterium tumefaciens. | rhizobium loti strains nzp2037 and nzp2213 were each found to contain a single large plasmid: prlo2037a (240 mdal) and prlo2213a (120 mdal), respectively. plasmid dna present in crude cell lysates of each strain and purified prlo2037a dna did not hybridize with pid1, a recombinant plasmid containing part of the nitrogen fixation (nif) region of r. meliloti, indicating that nif genes were not present on these plasmids. the transposon tn5 was inserted into prlo2037a and this plasmid was then trans ... | 1983 | 6313860 |
| mutational analysis of the virulence region of an agrobacterium tumefaciens ti plasmid. | forty-nine tn3 and tn5 transposition insertion mutations were introduced into the virulence region of the ptia6nc plasmid of agrobacterium tumefaciens. five tn5 transposition mutations from an earlier study (d. garfinkel and e. nester, j. bacteriol. 144:732-743, 1980) were also mapped more accurately. these mutations defined five separate loci within the virulence region. two tn3 insertions into one of these loci, vira, result in a strain which is only weakly virulent; however, a tn5 insertion i ... | 1983 | 6296058 |
| the conserved part of the t-region in ti-plasmids expresses four proteins in bacteria. | the t-region of ti-plasmids expresses four proteins (mol. wts. 74,000, 49,000, 28,000 and 27,000) in escherichia coli minicells. promoter activities are determined by sequences within the t-region, and the protein-coding regions map in that part of the t-region which is highly conserved in octopine and nopaline plasmids and which is responsible for shoot and root inhibition when expressed in plant cells. three of the regions expressed in bacteria correlate with three regions which are transcribe ... | 1983 | 11894956 |
| intergeneric transfer and exchange recombination of restriction fragments cloned in pbr322: a novel strategy for the reversed genetics of the ti plasmids of agrobacterium tumefaciens. | transmission of cole1/pmb1-derived plasmids, such as pbr322, from escherichia coli donor strains was shown to be an efficient way to introduce these plasmids into agrobacterium. this was accomplished by using e. coli carrying the helper plasmids pgj28 and r64drd11 which provide the cole1 mob functions and tra functions, respectively. for example, the broad host-range replication plasmid, pgv1150, a co-integrate plasmid between pbr322 and the w-type mini-sa plasmid, pgv1106, was transmitted from ... | 1983 | 11894957 |
| cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the t-dna. | insertion of the transposon tn5 into the t-region of the octopine ti plasmid of agrobacterium tumefaciens gives rise to crown gall tumors having altered morphology. three loci within the t-dna that control tumor morphology have been detected [garfinkel, d. j., simpson, r. b., ream, l. w., white, f. f., gordon, m. p. & nester, e. w. (1981) cell 27, 143-153]. they influence tumor size (tml), production of roots (tmr), or production of shoots (tms). cytokinin and auxin levels in such mutant tumors ... | 1983 | 16593270 |
| leucinopine, a characteristic compound of some crown-gall tumors. | an unusual compound has been found in crowngall tumors induced by those agrobacterium tumefaciens strains that utilize neither octopine nor nopaline. the compound has been isolated and shown by proton and carbon-13 nmr spectroscopy and by synthesis to be n(2)-(1,3-dicarboxypropyl)-l-leucine, which also exists in a cyclized (i.e., lactam) form. this compound, which we name "leucinopine," was not detected in octopine tumors, nopaline tumors, or the tumors induced by a. tumefaciens strains 181 and ... | 1983 | 16593328 |
| broad-host-range agrocin of agrobacterium tumefaciens. | eighteen strains of agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. of six agrocin-producing strains, one (d286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type ti plasmids. sensitivity to agrocin d286 was found to map in the 11- to 18-megadalton region of the nopaline ti plasmid ptic58. the agrocin was partially purified, and its physical characteristics were consistent with its being a nucleotide, ... | 1983 | 16346290 |
| interaction of agromyces ramosus with other bacteria in soil. | agromyces ramosus occurs in very high numbers in most soils and, based on studies of laboratory isolates, does not require host cells for growth. nevertheless, it attacked and destroyed most of the gram-positive and gram-negative bacterial species tested as possible host organisms. a. ramosus also attacked and destroyed saccharomyces cerevisiae. the possibility of attack on fungi was unclear. among the bacteria serving as hosts were the important soil species azotobacter vinelandii, rhizobium le ... | 1983 | 16346402 |
| development of dna-mediated transformation systems for plants. | the genetic engineering of plants by dna-mediated gene transfer requires that efficient transformation systems be developed. considerable progress has been made in manipulating the ti plasmid of agrobacterium tumefaciens as a vehicle for delivery of foreign genes into protoplasts of dicotyle-donous plants. part of the ti plasmid, the t-dna, can be incorporated into the genome of the host cell; the t-dna can carry a foreign dna sequence which co-integrates with it; under normal conditions, the tu ... | 1983 | 14544242 |
| induction and in vitro culture of soybean crown gall tumors. | induction of crown galls on 4-6 week old soybean (glycine max (l.) merr.) plants cultured in growth chambers was obtained with agrobacterium tumefaciens strains c58, t37 and ach5. the crown galls were isolated and cultured in vitro as sterile callus and liquid suspension cultures. transformation was tested by opine tests and by molecular hybridization of restricted cell dna with t-dna fragments. protoplasts were isolated from suspension cultures. transformed protoplasts regenerate cell walls, di ... | 1983 | 24258052 |
| from tumour to tuber; tumour cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (solanum tuberosum cv. 'maris bard'). | agrobacterium tumefaciens strains, known to induce tobacco crown galls that spontaneously develop shoots, were used to induce galls on cultured shoots of a tetraploid potato cultivar (solanum tuberosum cv. 'maris bard'). shoots also appeared spontaneously from the induced potato galls, although only after 2-4 months. the shoots were excised and cultured separately. some of these frequently developed side-shoots from their axillary buds. they did not form roots and they produced opines, a strong ... | 1983 | 24263772 |
| genetic identification of functions of tr-dna transcripts in octopine crown galls. | seventeen in vivo and in vitro insertion mutations in the tr-region of the octopine ti plasmid b6s3 of agrobacterium tumefaciens were constructed. they include mutations in each of the five tr transcripts. all mutants retained their oncogenic properties and induced tumors with wild-type morphology. determination of opines in tumors induced by mutant agrobacterium strains showed that production of mannopine and agropine are tr-linked traits. two transcripts are necessary for the synthesis of mann ... | 1984 | 16453491 |
| t-dna of a crown gall tumor is organized in nucleosomes. | nucleosomes were isolated from chromatin of suspension cultured cells of nicotiana tabacum var. white burley, which were either habituated or transformed by agrobacterium tumefaciens, strain t37. chromatin repeat length in both types of tissue was identical and can be estimated to be 195 +/- 10 bp. using southern transfer of nucleosomal dna and hybridization with cloned nick-translated hindiii fragments of pti c58 we show that the t-dna originating from the ti-plasmid of a. tumefaciens is organi ... | 1984 | 16453504 |
| delivery of t-dna from the agrobacterium tumefaciens chromosome into plant cells. | the intact t-region of the b6ti plasmid of agrobacterium tumefaciens was stepwise cloned into a site in transposon tn3. in this way a suitable vehicle (tn1882) was obtained for translocating the t-region to different replicons, i.e., to other plasmids or the chromosome. the incp plasmid r772::tn1882 conferred tumorigenicity on agrobacterium if the virulence genes were provided in trans in the same cell. this result showed that the t-region present on tn1882 was transferred efficiently to plant c ... | 1984 | 16453562 |
| isolation of a dual plant promoter fragment from the ti plasmid of agrobacterium tumefaciens. | the two most abundant transcripts derived from tr-dna within plant cells transformed by an octopine strain of agrobacterium tumefaciens arise from divergent transcription, both originating within an 500 bp section of the t-dna. using a combination of subcloning and exonuclease digestion, a 479-bp dna fragment, directly flanked by the initiation codons for the two adjacent open reading frames, was isolated. the resulting dna fragment was fused, in both orientations, to the neomycin phosphotransfe ... | 1984 | 16453574 |
| an agrobacterium-transformed cell culture from the monocot asparagus officinalis. | cultured stem fragments from the monocotyledonous plant asparagus officinalis infected by the oncogenic bacterium agrobacterium tumefaciens developed tumorous proliferations. this tissue was propagated in vitro on hormone-free culture medium. the t-dna-encoded markers nopaline and agrocinopine were unambiguously detected in these tissues. the data demonstrate that stable t-dna transfer as well as expression of t-dna genes is possible in at least some monocotyledonous plants. this opens new possi ... | 1984 | 16453585 |
| the limited host range of an agrobacterium tumefaciens strain extended by a cytokinin gene from a wide host range t-region. | the host range of agrobacterium tumefaciens strain lba649 (ptiag57) is limited to grapevine and a few other plant species. its host range was extended through the introduction of the t-region from the wide host range octopine plasmid ptiach5. in contrast, r prime plasmids harboring the entire wide host range virulence region were unable to achieve this effect. via site-directed mutagenesis a search was performed to identify the t-dna genes which were responsible for the observed host range exten ... | 1984 | 16453586 |
| plasmid-linked nif and "nod" genes in fast-growing rhizobia that nodulate glycine max, psophocarpus tetragonolobus, and vigna unguiculata. | forty-nine fast-growing rhizobium strains from the nodules of 26 different tropical legume genera were screened to find isolates that would (i) nodulate, e.g., winged beans, so producing large nodules for rna and protein isolation; (ii) also nodulate various small-seeded legumes, thus allowing screening of large numbers of mutants; and (iii) harbor plasmids containing nif structural genes as well as other functions involved in nodulation. on the basis of six different criteria, this rhizobial gr ... | 1984 | 16593465 |
| identification of a cloned cytokinin biosynthetic gene. | a small region of the ti plasmid (the tmr locus), thought to be involved in phytohormone metabolism in agrobacterium tumefaciens-transformed plant tissue, was cloned and expressed in escherichia coli. by enzyme assay, the tmr locus was shown to encode isopentenyltransferase, an enzyme that catalyzes the first step in cytokinin biosynthesis. | 1984 | 16593495 |
| evidence for host genome involvement in cytokinin metabolism by male and female cells of mercurialis annua transformed by strain 15,955 of agrobacterium tumefaciens. | when male and female individuals of a dioecious species mercurialis annua l. were inoculated with the same strain of agrobacterium tumefaciens (15,955), the corresponding tumor tissues of each sex clearly differed in their endogenous cytokinin content; only the male tumors had a morphogenetic feminizing effect on male flowers.in male tumor tissues, zeatin (z) in higher quantity than ribosyl-zeatin (rz) became the major metabolite in contrast with the general situation for crown-galls; the female ... | 1984 | 16663368 |
| primary action of indole-3-acetic acid in crown gall tumors: increase of solute uptake. | exogenously added indole-3-acetic acid at a concentration of 100 micromolars stimulates d-glucose uptake (or 3-o-methyl-d-glucose uptake) by 25% in crown gall tumors induced on potato tuber tissue by agrobacterium tumefaciens strain c 58. the titration of the endogenous iaa with the auxin antagonist 2-naphthaleneacetic acid at 100 micromolars reduces d-glucose uptake by about 80%. the apparent inhibition constant k(i) is 21 micromolars. other auxin antagonists like 1-naphthoxyacetic acid and 2-( ... | 1984 | 16663625 |
| quantitation of cytokinins in biological samples using antibodies against zeatin riboside. | the cross-reactivity of antibodies elicited in rabbits against zeatin riboside, to a wide range of naturally occurring cytokinins, was examined. as well as to zeatin riboside, the antisera cross-reacted to a considerable extent with zeatin, lupinic acid, zeatin-9-glucoside, zeatin riboside 5'-monophosphate and to a much lesser, but measurable extent, with dihydrozeatin riboside and dihydrozeatin. chromatographic methods were devised which allowed separation of all these cross-reactive compounds. ... | 1984 | 16663745 |