Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| mortality induced by adoptive immunity in junin virus-infected athymic mice. | the effect of normal or sensitized spleen cell transfer from syngeneic euthymic mice to junin virus-infected suckling athymic mice was studied. transfer was performed 1 or 7 days after infection. in both cases, an acute lethal disease developed 6-11 days after transfer. the mortality reached 100% in all infected groups receiving normal or sensitized splenocytes, while it was negligible for different control groups of athymic mice. transfer of normal or sensitized splenocytes was unable to signif ... | 1988 | 2842272 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| ribavirin effect on experimental junin virus-induced encephalitis. | junin virus, the etiological agent of argentine hemorrhagic fever, produces in man a disease mainly characterized by hemorrhagic alterations, commonly accompanied by neurological symptoms, and leading to 10% mortality. intracerebral inoculation in 10-day-old rats or intraperitoneal inoculation in 2-day-old rats leads to high mortality due to severe encephalitis. here, the effect of ribavirin on these experimental models was tested in order to evaluate the degree of protection achieved against ne ... | 1988 | 2846772 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| lectin affinity of junin virus glycoproteins. | we studied the binding of junin virus (arenaviridae) glycoproteins, g1 and g2, to two insolubilized lectins. the results showed that mannose, n-acetyl-glucosamine and galactose residues were exposed on g2, while only the latter predominated on g1. heterogeneity of carbohydrate chains was found in g2, the only glycoprotein that was iodinated by the lactoperoxidase method. | 1988 | 2849965 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| mouse splenocyte transfer effect depends on donor's junin virus infection stage. | splenocytes from junin-virus-persistently-infected euthymic mice taken at 45 days postinfection seemed unable to induce overt signs of disease, to cause death, or to modify brain viral levels when transferred to athymic junin-virus-infected mice. findings differed sharply when the same recipients were transferred with splenocytes taken at 6 or 30 days postinfection from immunocompetent mice infected in adult life, since mortality reached 80 or 50%, respectively, and brain viral titers were signi ... | 1988 | 3260227 |
| in vivo junin virus-mouse macrophages interaction. | the role of mononuclear phagocytic cells in extraneural infection of the mouse with junin virus (jv) was studied. endpoint susceptibility (4 days of life) was evaluated by intraperitoneal (i.p.) inoculation of suckling mice. by means of immunofluorescence (if) and c3 receptor assays, it was found that macrophages were permissive to viral replication in vivo and fostered the recruitment of inflammatory cells as evidenced by the absence of c3 marker. in support, in vitro infection failed to induce ... | 1988 | 2902768 |
| suppressor t-cell population induced by junin virus in adult mice. | intracerebral (i.c.) junin virus (jv) infection of adult balb/c mice is characterized by the absence of morbidity and a low mortality (barely 8-10%). in contrast, the suckling mouse model exhibits almost 100% mortality following central nervous system (cns) alterations consistent with a delayed-type hypersensitivity (dth)-like immune response. besides, jv infection of adult (resistant) mice leads to immunosuppression of dth to unrelated antigens. here we present evidence demonstrating that such ... | 1988 | 2970429 |
| antigenic relationships between attenuated and pathogenic strains of junin virus. | antigenic relationships between attenuated and pathogenic strains of junin virus (jv) were investigated. five strains of either human or rodent origin were tested by cross-neutralization assay with hyperimmune antisera, raised in rabbits, against each strain. polyclonal antisera could be used to distinguish among these jv strains, as the titer values differed significantly with ratios of homologous to heterologous titers, which ranged from 1.3 to 22.3. this demonstrates, independent of their vir ... | 1989 | 2466103 |
| junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. | twenty-one monoclonal antibodies reactive with junin virus structural proteins were produced and characterized. using radioimmunoprecipitation and western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. in radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35k structu ... | 1989 | 2471803 |
| reduced virulence of a junin virus mutant is associated with restricted multiplication in murine cells. | c167, a mutant derived from the xjc13 strain of junin virus, is highly attenuated in its pathogenic properties for newborn mice. whereas 10(2).pfu of xjc13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. survival of mice infected with c167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cel ... | 1989 | 2479190 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| nucleocapsid protein gene of junin arenavirus (cdna sequence). | 1989 | 2552421 | |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| [rapid serologic diagnosis of argentinian hemorrhagic fever in whole blood]. | the usefulness of a method for detection of antibodies against junin virus in whole blood was tested. n: nih adult mice were inoculated with 10(3) pfu of attenuated xj-clon 3 junin virus strain by intraperitoneal route and blood was obtained by retro-orbital puncture at 21 days post-infection. one blood aliquot (50 microliters) was collected in tubes containing a stabilizer solution for whole blood and another was processed for serum obtention. immunofluorescent antibodies were tested on spot sl ... | 1989 | 2559426 |
| astrocytic reaction predominance in chronic encephalitis of junin virus-infected rats. | junin virus antigen distribution and astrocytic reaction to prolonged infection were characterized in rat brain by the pap technique. during the acute stage of neurologic disease following intracerebral inoculation, junin antigen was detected in 100% of animals, strongly in most neurons but also to a much lesser degree in scattered astrocytes, dropping to 20% of rats at 540 days postinfection. initially labeled in all brain areas, viral antigen gradually disappeared from hippocampus but persiste ... | 1989 | 2559956 |
| [protection against encephalitis in rats caused by a pathogenic strain of the junin virus, using peripheral inoculation of an attenuated strain]. | argentine hemorrhagic fever manifests itself in man either subclinically or in hemorrhagic or neurological forms, mortality reaching 20%. although candid 1 strain is undergoing pilot trials, current therapy still resorts to convalescent serum administration. a neurological model was used to evaluate protection conferred by the attenuated xjc13 junin virus strain. newborn rats inoculated intraperitoneally (ip) prove resistant, whereas 8-12 day-old animals infected by intracerebral route with the ... | 1989 | 2562073 |
| protection of guinea pigs against experimental argentine hemorrhagic fever by purified human igg: importance of elimination of infected cells. | antibody-containing plasma from patients recovered from argentine hemorrhagic fever (ahf) is of proven value in treatment of the acute disease, but the possibility of transmitting blood-borne organisms such as hiv and hepatitis virus detracts from this approach. purified human immune plasma fractions igg1,2,4, igg1,2,3,4 and f(ab')2 neutralized junin virus in vitro. igg1,2,3,4 and igg1,2,4 lysed (in the presence of complement) cells infected with junin virus, and protected infected guinea pigs f ... | 1990 | 1965845 |
| protection of guinea pigs against experimental argentine hemorrhagic fever by purified human igg: importance of elimination of infected cells. | antibody-containing plasma from patients recovered from argentine hemorrhagic fever (ahf) is of proven value in treatment of the acute disease, but the possibility of transmitting blood-borne organisms such as hiv and hepatitis virus detracts from this approach. purified human immune plasma fractions igg1,2,4, igg1,2,3,4 and f(ab')2 neutralized junin virus in vitro. igg1,2,3,4 and igg1,2,4 lysed (in the presence of complement) cells infected with junin virus, and protected infected guinea pigs f ... | 1990 | 1965845 |
| [intracerebral infection of athymic mice with an attenuated strain of junín virus]. | infection of newborn immunocompetent (nu/+) mice with the xj prototype strain of junin virus, etiological agent of argentine hemorrhagic fever, produces a lethal meningo-encephalitis due to cellular immune response. the same strain inoculated into athymic (nu/nu) mice produces an asymptomatic persistent infection. the purpose of this work was to determine the nu/nu mice response when infected with xjcl3, an attenuated junin virus strain, since this strain behaves differently to the pathogenic pr ... | 1990 | 1966306 |
| [immunopathology induced in the rat by junin virus]. | intra-cerebral infection of the 10-day-old rat with the xj prototype strain of junin virus induces an immunopathological encephalitis with 100% mortality. in contrast with previous observations, our present work with antithymocyte serum (ats) demonstrates a pathological role for the cellular immune response in this experimental model. as regards ats treatment, 3 schedules were employed, the most efficient being daily 0.01 ml/g weight doses from day -1 to day +9, then +12, +14 and +16, taking day ... | 1990 | 1966503 |
| [blood parameters variation in calomys musculinus infected with junin virus, strain xjcl3]. | the aim of this study was to analyze the alterations in homeostasis induced by junin virus during acute and persistent infection of c. musculinus. virus presence in brain, hematological response and glycemia levels were evaluated. newborn c. musculinus inoculated with 4000 dl50 of junin virus, strain xjcl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. virus was isolated from brain starting day 6 post-infection (fig. 1) and the peak titer (10(8) dl50/ml) was r ... | 1990 | 1966622 |
| [blood parameters variation in calomys musculinus infected with junin virus, strain xjcl3]. | the aim of this study was to analyze the alterations in homeostasis induced by junin virus during acute and persistent infection of c. musculinus. virus presence in brain, hematological response and glycemia levels were evaluated. newborn c. musculinus inoculated with 4000 dl50 of junin virus, strain xjcl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. virus was isolated from brain starting day 6 post-infection (fig. 1) and the peak titer (10(8) dl50/ml) was r ... | 1990 | 1966622 |
| contrasuppressor cells induced by junin virus. | intracerebral inoculation of junin virus (jv) in all susceptible mouse models available induces a lethal meningoencephalitis compatible with a delayed-type hypersensitivity (dth) immune response. in contrast, adult balb/c mice prove resistant to infection and no dth response is seen. jv inoculation in adult balb/c mice induces dth suppression to unrelated antigens such as sheep red blood cells. (srbc). this suppression is mediated by jv-induced t cells (tsv), which are operative from 1 to 24 day ... | 1990 | 1970806 |
| junin virus replication in peripheral blood mononuclear cells of patients with argentine haemorrhagic fever. | to study the relationship of junin virus (jv) to populations of peripheral blood mononuclear cells (pbmc) from patients with argentine haemorrhagic fever (ahf), blood samples were obtained during the acute period of disease and cultured as total, adherent, and non-adherent cell populations. jv was sequentially sought in these cell populations by using an infectious centre (ic) assay, whereas free jv in the supernatants was evaluated by plaque formation. ic were obtained in cultures of total pbmc ... | 1990 | 1975726 |
| hantavirus infection in laboratory and wild rodents in argentina. | serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ... | 1990 | 1981381 |
| hantavirus infection in laboratory and wild rodents in argentina. | serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ... | 1990 | 1981381 |
| a plasma inhibitor of platelet aggregation in patients with argentine hemorrhagic fever. | hemorrhage in patients with lassa fever is associated with the presence of a circulating plasma inhibitor of platelet aggregation. this study was to determine whether patients with argentine hemorrhagic fever (ahf) develop a similar inhibitor. normal platelets showed significantly weaker aggregation responses to a sub-maximal dose of adenosine diphosphate (adp) when mixed with plasma from 10 patients with ahf (mean percent of control +/- 1 se = 57.2 +/- 6.7%) compared to those mixed with plasma ... | 1990 | 2160197 |
| junin virus-induced suppressor cells are effective only on the efferent limb of the delayed-type hypersensitivity response. | junin virus (jv), the etiological agent of argentinian hemorrhagic fever, induces high mortality in the suckling balb/c mouse, which correlates with delayed-type hypersensitivity (dth)-like immune response. in contrast, the adult mouse is resistant to infection, and no dth response can be detected. an antigen-nonspecific dth suppressor t-cell activity induced by jv has been described that may be related to adult mouse survival. in this report, we present evidence supporting the inability of such ... | 1990 | 2160514 |
| a mouse attenuated mutant of junin virus with an altered envelope glycoprotein. | the xjc13 strain of junin virus (jv) and the mouse-attenuated mutant c167 showed different gp38 peptide mapping after limited proteolysis with ficin and papain; viral infectivity of both viruses also exhibited a different susceptibility to protease treatment. a correlation between envelope glycoprotein alteration and jv virulence in neonatal mice is proposed. | 1990 | 2162164 |
| a refined complement-enhanced neutralization test for detecting antibodies to junin virus. | a refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against junin (argentine hemorrhagic fever) virus. the assay measured neutralizing antibodies after natural as well as vaccine-induced junin virus infections. among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. enhanced sensitivity was achieved by using a standardized complement source (vs human o ... | 1990 | 2170437 |
| a comparison of junin virus strains: growth characteristics, cytopathogenicity and viral polypeptides. | the growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain xj of junin virus (jv), its attenuated derivative xjc13 and another naturally attenuated jv strain, iv4454, were comparatively studied. iv4454 and xjc13 viruses showed the highest and lowest cytopathology for vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. the kinetics and electrophoretic patterns of viral polypeptides in infected c ... | 1990 | 2177565 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| method for improving accuracy of virus titration: standardization of plaque assay for junin virus. | titrating infective virus is one of the most important and common techniques in virology. however, after many years of widespread use, the parameters governing the accuracy of titration values are still not well understood. it was found that under conditions currently used for virus titration, only a small percentage of virus in the inoculum is adsorbed onto the cells and thereby detected in the titration assay. the objective of our work was to establish the conditions for a plaque assay which c ... | 1990 | 1707890 |
| junin virus-induced non-specific suppressor cells interact with unrelated antigen-specific suppressor cells. | junin virus (jv) infection of adult (resistant) balb/c mice induces antigen non-specific delayed-type hypersensitivity (dth) suppressor t cells, termed tsv, bearing the thy-1+, ly-1+2- phenotype. these cells may be related to survival to infection since dth reaction is associated with lethal meningoencephalitis. employing several xenogeneic red blood cell (rbc) sensitization schedules to induce different cell subpopulations, we have attempted to establish the target of jv-induced suppressor cell ... | 1991 | 1718851 |
| a triple staining procedure to evaluate phagocytic role of differentiated astrocytes. | to determine whether phagocytic activity is affected by a viral infection known to induce astrocyte differentiation, a triple procedure (pap labeling for gfap, pas reaction for added baker's yeast cells and hematoxylin for nuclear staining of the whole monolayer) was applied to junin virus-inoculated cultures, as well as matched controls. the three-step staining simplified yeast cell count for subsequent statistical analysis, which discerned preferential uptake by differentiated rather than imma ... | 1991 | 1724055 |
| microgeographic distribution of allele frequencies in populations of calomys laucha (rodentia, cricetidae). | the microgeographic differentiation of allelic frequencies was analysed in two population samples of calomys laucha, collected in a grid set in cultivated fields at laguna larga (córdoba province, argentina). one of the samples (n = 79) was obtained in may (autumn), when the population density is at a maximum, and the other (n = 85) in november (spring), at the onset of reproductive activity. fst values for the november sample show significant differences between the six groups over which the sa ... | 1991 | 1880049 |
| microgeographic distribution of allele frequencies in populations of calomys laucha (rodentia, cricetidae). | the microgeographic differentiation of allelic frequencies was analysed in two population samples of calomys laucha, collected in a grid set in cultivated fields at laguna larga (córdoba province, argentina). one of the samples (n = 79) was obtained in may (autumn), when the population density is at a maximum, and the other (n = 85) in november (spring), at the onset of reproductive activity. fst values for the november sample show significant differences between the six groups over which the sa ... | 1991 | 1880049 |
| cytotoxicity and cytoadherence of human and murine polymorphonuclear leukocytes against junin virus infected targets in the absence of anti-viral antibody and complement. | human and murine polymorphonuclear leukocytes (pmn) lysed l cells infected with junin virus (jv) in an in vitro system free of antiviral antibody and complement. infected vero cells proved to be resistant to the cytolytic effect. murine pmn showed an increased adherence on jv-infected l cells but did not attach to vero cells. the opposite was observed with human pmn, which did adhere to infected vero cells but not to infected l cells. these results indicate that pmn activity may be an early mech ... | 1991 | 1649909 |
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |
| toward a vaccine against argentine hemorrhagic fever. | a vaccine against argentine hemorrhagic fever, the "mal de los rastrojos" of the pampas, has been a dream of physicians and scientists involved with the disease since its recognition in the 1950s. several killed and live immunogens have been produced and tested in pursuit of this goal, none of which has proved suitable for widespread human use. recently, a new live-attenuated junin virus vaccine, candid #1, was developed through a cooperative international effort. testing conducted to date indic ... | 1991 | 1654168 |
| molecular organization of junin virus s rna: complete nucleotide sequence, relationship with other members of the arenaviridae and unusual secondary structures. | in this study, overlapping cdna clones covering the entire s rna molecule of junin virus, an arenavirus that causes argentine haemorrhagic fever, were generated. the complete sequence of this 3400 nucleotide rna was determined using the dideoxynucleotide chain termination method. the nucleocapsid protein (n) and the glycoprotein precursor (gpc) genes were identified as two non-overlapping open reading frames of opposite polarity, encoding primary translation products of 564 and 481 amino acids, ... | 1991 | 1654373 |
| experimental infection of suckling mice with a host range mutant of junin virus. | experimental infection of three mouse strains with a non-pathogenic mutant of junin virus named cl67 was compared with respect to the parental xjcl3 strain. after intracerebral (ic) or intraperitoneal inoculation, xjcl3 was highly virulent for 2 day-old c3h/hej, of1, and balb/cj mouse strains, whereas its derivative cl67 was attenuated. survival of the cl67-infected mouse was associated with a restricted replication at the site of inoculation which would impair spread of virus. thus, the reduced ... | 1991 | 1658215 |
| [lysosomotropic compounds inhibiting the multiplication of junin virus]. | a number of lysosomotropic compounds of diverse chemical structure, ammonium chloride, procaine and chlorpheniramine, were found to inhibit the infection of vero cells by junín virus. viral replication was almost totally inhibited by 15 mm ammonium chloride, when added either before or within the first hour of infection and a significant inhibition (97.8%) was observed when it was added 8 hours after infection. these results agree with a model which postulates that arenavirus entry occurs by rec ... | 1991 | 1667699 |
| rapid vascular clearance of two strains of junin virus in calomys musculinus: selective macrophage clearance. | clearance of junin (jun) virus strains with different virulence for calomys musculinus (cm) was followed using the candid #1 virulent and cbafha 5069 attenuated strains. in addition, virulent virus albino mice (am) were included as control host and venezuelan equine encephalitis (vee-vi) virus as control virus. the virus inoculum (vo) and the blood samples (vt) obtained at different times post-inoculation (p.i.) were titrated on vero cells and the cleared plaque forming-units (pfu) were calculat ... | 1991 | 1681712 |
| rapid vascular clearance of two strains of junin virus in calomys musculinus: selective macrophage clearance. | clearance of junin (jun) virus strains with different virulence for calomys musculinus (cm) was followed using the candid #1 virulent and cbafha 5069 attenuated strains. in addition, virulent virus albino mice (am) were included as control host and venezuelan equine encephalitis (vee-vi) virus as control virus. the virus inoculum (vo) and the blood samples (vt) obtained at different times post-inoculation (p.i.) were titrated on vero cells and the cleared plaque forming-units (pfu) were calculat ... | 1991 | 1681712 |
| [new findings on junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in argentina]. | in conjunction with field trials for a vaccine against argentine hemorrhagic fever (ahf), small mammals were trapped during a 28-month period (1 november 1987 to 13 march 1990) in 3 epidemiologically defined areas of the central argentine pampas: northern and central buenos aires provinces were included in the ahf "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern santa fe province is the current high-incidence area for ahf; the nonendemic a ... | 1991 | 7476104 |
| immunoperoxidase tracing of junin virus neural route after footpad inoculation. | to determine the pathway adopted by peripherally inoculated junin virus (jv) to reach the cns, rat tissues were serially harvested to trace the sequence of viral progression from right hind footpad to brain. immunoperoxidase (pap) labeling of viral antigen, concomitantly with infectivity assays and histological examination of each selected sample, were carried out. as from the 2nd week post-infection (pi), neurological disease inducing 100% mortality at 1 month was evident. at day 5 pi, viral an ... | 1992 | 1309637 |
| current progress towards vaccines for arenavirus-caused diseases. | the arenaviruses are primarily viruses of rodents, but some members of the group cause severe disease (argentine and bolivian haemorrhagic fevers and lassa fever) when transmitted to humans in the specific areas of the world where they are enzootic. current research of relevance to the provision of vaccines against these diseases, which highlights many of the problems encountered generally in the development of vaccines, is reviewed here. although one of the classical approaches to vaccine produ ... | 1992 | 1311492 |
| correlation between cyclophosphamide-induced viral susceptibility and depletion of junin virus-induced suppressor populations. | in contrast to lymphocytic choriomeningitis virus, another arenavirus, junin virus (jv), the etiologic agent of argentine hemorrhagic fever, when inoculated into suckling mice, induces lethal meningoencephalitis characterized by a delayed-type hypersensitivity (dth)-like immune response. however, the adult balb/c mouse is resistant to infection and no dth reaction can be seen. this different viral sensitivity may be related to the development of an antigen non-specific dth-suppressor cell pathwa ... | 1992 | 1315368 |
| experimental neuroinvasiveness of wild and laboratory junin virus strains. | the neuroinvasiveness of candid 1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus (cm), of different ages inoculated by a non-neural route. infectivity in brain, blood and organs, as well as lethality, were determined. the results with the 3 hosts indicate that junin virus neuroinvasiveness is virus-strain-dependent, host species- and age-dependent, with the candid ... | 1992 | 1329167 |
| experimental neuroinvasiveness of wild and laboratory junin virus strains. | the neuroinvasiveness of candid 1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus (cm), of different ages inoculated by a non-neural route. infectivity in brain, blood and organs, as well as lethality, were determined. the results with the 3 hosts indicate that junin virus neuroinvasiveness is virus-strain-dependent, host species- and age-dependent, with the candid ... | 1992 | 1329167 |
| detection of junin virus by the polymerase chain reaction. | argentine hemorrhagic fever is an often fatal human disease caused by junin virus, an rna-containing virus and member of the arenavirus family. this virus was detected in vitro by the polymerase chain reaction (pcr) procedure. a pair of junin virus-specific pcr dna oligonucleotide primers and an oligonucleotide probe were designed from a known portion of the viral rna sequence. rna was isolated from junin virus-infected monkey kidney cells and used to produce complementary dna (cdna) by reverse ... | 1992 | 1331150 |
| peripheral blood leukocytes of patients with argentine hemorrhagic fever as effectors of antibody-dependent cell-cytotoxicity. | peripheral blood mononuclear cells (pbmc) and polymorphonuclear leukocytes (pmnl) of patients with argentine hemorrhagic fever (ahf) were tested as effectors (e) of antibody-dependent cell cytotoxicity (adcc). 51cr labeled chicken red blood cells (crbc) coated with anti-crbc or normal rabbit serum were used as targets (t). three adcc assays were performed with both effectors from patients: on admission (i), 4 days after the transfusion of immune plasma (ii), and 30 days after the clinical onset ... | 1992 | 1331313 |
| peripheral blood leukocytes of patients with argentine hemorrhagic fever as effectors of antibody-dependent cell-cytotoxicity. | peripheral blood mononuclear cells (pbmc) and polymorphonuclear leukocytes (pmnl) of patients with argentine hemorrhagic fever (ahf) were tested as effectors (e) of antibody-dependent cell cytotoxicity (adcc). 51cr labeled chicken red blood cells (crbc) coated with anti-crbc or normal rabbit serum were used as targets (t). three adcc assays were performed with both effectors from patients: on admission (i), 4 days after the transfusion of immune plasma (ii), and 30 days after the clinical onset ... | 1992 | 1331313 |
| metal-based formulations with high microbicidal activity. | substances were evaluated for their relative potencies in inactivating junin virus, escherichia coli, and spores of bacillus subtilis. under the conditions of our test, glutaraldehyde was the most efficient agent among all substances currently recommended for disinfecting and sterilizing medical devices. either copper or iron ions by themselves were able to inactivate virus with an efficiency similar to that of substances currently used for disinfection and sterilization. the microbicidal effect ... | 1992 | 1332611 |
| a longitudinal study of junin virus activity in the rodent reservoir of argentine hemorrhagic fever. | we monitored junin virus (jv) activity in rodent populations for 30 months at seven mark-recapture grids located in agricultural fields and adjacent roadsides and fence lines in endemic and nonendemic areas of argentine hemorrhagic fever. blood and oral swabs taken from rodents captured at five-week intervals were analyzed by enzyme-linked immunosorbent assay for jv antigen (ag). calomys laucha and c. musculinus were the most frequently captured rodents, making up 47% and 22% of captures, respec ... | 1992 | 1335214 |
| a longitudinal study of junin virus activity in the rodent reservoir of argentine hemorrhagic fever. | we monitored junin virus (jv) activity in rodent populations for 30 months at seven mark-recapture grids located in agricultural fields and adjacent roadsides and fence lines in endemic and nonendemic areas of argentine hemorrhagic fever. blood and oral swabs taken from rodents captured at five-week intervals were analyzed by enzyme-linked immunosorbent assay for jv antigen (ag). calomys laucha and c. musculinus were the most frequently captured rodents, making up 47% and 22% of captures, respec ... | 1992 | 1335214 |
| candid no. 1 argentine hemorrhagic fever vaccine protects against lethal junin virus challenge in rhesus macaques. | the protective efficacy of candid no. 1, a live-attenuated vaccine against argentine hemorrhagic fever (ahf), was evaluated in non-human primates. twenty rhesus macaques immunized 3 months previously with graded doses of candid no. 1 (16-127, 000 pfu), as well as 4 placebo-inoculated controls, were challenged with 4.41 log10 pfu of virulent p3790 strain junin virus. all controls developed severe clinical disease; 3 of 4 died. in contrast, all vaccinated animals were fully protected; none develop ... | 1992 | 1338783 |
| candid no. 1 argentine hemorrhagic fever vaccine protects against lethal junin virus challenge in rhesus macaques. | the protective efficacy of candid no. 1, a live-attenuated vaccine against argentine hemorrhagic fever (ahf), was evaluated in non-human primates. twenty rhesus macaques immunized 3 months previously with graded doses of candid no. 1 (16-127, 000 pfu), as well as 4 placebo-inoculated controls, were challenged with 4.41 log10 pfu of virulent p3790 strain junin virus. all controls developed severe clinical disease; 3 of 4 died. in contrast, all vaccinated animals were fully protected; none develop ... | 1992 | 1338783 |
| aerosol infection of rhesus macaques with junin virus. | the purpose of our work was to determine if aerosols of junin virus can infect rhesus macaques and if the disease is the same as that produced by virus inoculated parenterally. the 6 macaques exposed to the virus by aerosol became acutely ill during the 3rd week after exposure, and all died. three died by day 21, while the remainder died after 1 month. junin virus was found primarily in visceral organs of those animals dying before 21 days after infection and in the central nervous system tissue ... | 1992 | 1371270 |
| aerosol infection of rhesus macaques with junin virus. | the purpose of our work was to determine if aerosols of junin virus can infect rhesus macaques and if the disease is the same as that produced by virus inoculated parenterally. the 6 macaques exposed to the virus by aerosol became acutely ill during the 3rd week after exposure, and all died. three died by day 21, while the remainder died after 1 month. junin virus was found primarily in visceral organs of those animals dying before 21 days after infection and in the central nervous system tissue ... | 1992 | 1371270 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| [present status of zoonotic hemorrhagic fevers of south america]. | since 1958, the geographical distribution of argentine hemorrhagic fever (ahf) has especially extended non only into the province of buenos aires but also towards the provinces of santa fe and cordoba, leading to an estimated population at risk of about 1.2 m inhabitants. recent epidemiological field studies has confirmed the major role of calomys musculinus and c. laucha rodents in both transmission to man and conservation of junin virus in nature. however, the human infection may result essent ... | 1993 | 7819800 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| heterogeneity and stability characteristics of candid 1 attenuated strain of junin virus. | fourteen clones of candid 1 strain of junin virus were studied in terms of their attenuation and stability for 9 days-old mice. the cluster analysis of the efficiency of lethal infection, namely the virulence (lethality) indicates (pfu/ld50) of the clones showed that the strain population is heterogeneous. it is constituted by subpopulations with lower and higher virulence as compared to the parent strain. moreover, one or two passages through mice and calomys musculinus, the main junin virus re ... | 1993 | 8105651 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| in vitro selection of junin virus antigenic variants. | the establishment of an experimental persistent infection with junin virus, the aetiological agent of argentine hemorrhagic fever, involves the emergence of antigenic variants in brain and blood of the cricetid calomys musculinus. we demonstrate that antigenic variants can also be isolated in vitro under the selective pressure of polyclonal antibodies and from a long-term infected c. musculinus primary embryo fibroblast culture. the participation of neutralizing antibodies and host cells in the ... | 1993 | 8382041 |
| a simple nucleic acid amplification assay for the rapid detection of junín virus in whole blood samples. | argentine hemorrhagic fever (ahf) is an endemoepidemic disease with cardiovascular, renal and neurologic alterations acquired in the richest farming land in argentina. it is caused by junín virus, one of the few human pathogenic arenaviruses. the s rna of junín virus has been molecularly cloned and its nucleotide sequence determined in our laboratory. this information was used to develop a rapid nucleic acid-based diagnostic test commensurate with the low viraemia detected in ahf patients. junín ... | 1993 | 8383393 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| safety and immunogenicity of a live-attenuated junin (argentine hemorrhagic fever) vaccine in rhesus macaques. | the safety and immunogenicity of candid #1, a live-attenuated junin virus vaccine, were evaluated in rhesus macaques. candid #1 was inoculated subcutaneously in graded doses ranging from 16 to 127,200 plaque-forming units (pfu) into four groups of five animals each; four controls received saline. there was no significant effect of the immunization on any physical, hematologic, or biochemical parameter measured. junin virus was recovered by cocultivation from peripheral blood mononuclear cells (p ... | 1993 | 8385886 |
| replication and physical parameters important for preparing purified junin virus. | junin virus (jv) is an arenavirus and the causative agent of argentine hemorrhagic fever (ahf), an often fatal human disease. the attenuated strain xj-clone 3 (xjc13) of jv, after being tested in humans, has been considered a promising vaccine. we found that synthesis of jv xjc13 reaches a peak 2 days after infection and the kinetics of synthesis are little affected by the multiplicity of infection (moi) in a range from 0.125 to 1.00. virus synthesis is sensitive to actinomycin d, indicating tha ... | 1993 | 8388396 |
| the effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of junin virus. | the effects of specific inhibitors of glycoprotein trimming reactions on junin virus (jv) replication were investigated. bromoconduritol, an inhibitor of glucosidase ii, significantly reduced infective virus production (de50: 1.1 mm) and viral protein expression. neither 1-deoxynojirimycin, an inhibitor of both glucosidases i and ii, nor 1-deoxymannojirimycin and swainsonine, inhibitors of mannosidase i and ii, respectively, showed any activity against jv multiplication. these results are the fi ... | 1993 | 8391500 |
| antiviral treatment of argentine hemorrhagic fever. | argentine hemorrhagic fever is a systemic viral disease caused by junin virus, with a mortality of 15-30% in untreated individuals. current specific therapy is highly effective in reducing mortality, and consists of the early administration of immune plasma in defined doses of specific neutralizing antibodies per kg of body weight. however, several reasons suggest the need to investigate alternative therapies. ribavirin, a broad spectrum antiviral agent, is effective in the treatment of other vi ... | 1994 | 8141590 |
| intracellular processing and transport of junin virus glycoproteins influences virion infectivity. | the influence of glycoprotein processing, cleavage and transport on junin virus (jv) infectivity was investigated using monensin combined with lectin binding assays. yields of extracellular virus were more significantly reduced than cell-associated virus, indicating that monensin inhibited the transport of infectious virus to the extracellular space on a late stage of the replicative cycle. shown by lectin reactivity and immunoprecipitation, the intracellular processing of jv glycoproteins invol ... | 1994 | 7856317 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| prevalence of infection with junin virus in rodent populations in the epidemic area of argentine hemorrhagic fever. | we report the results of indirect fluorescent antibody screening for antibody to junin virus in 1,101 sera from small mammals captured on two mark-recapture grids in the epidemic area of argentine hemorrhagic fever. twenty-six of 29 seropositive animals were the cricetid rodent calomys musculinus, for a 30-month prevalence of 7.9% in that species. combining these data with previously published data on antigen detection provided an estimated total prevalence of infection of 10.9% for this, the pr ... | 1994 | 7985747 |
| latin american hemorrhagic fever. | several viral and bacterial agents are responsible for hemorrhagic fever in latin america, but there are three agents that are only present in this region: (1) the junin virus, (2) the machupo virus, and (3) the guanaritovirus, members of the tacaribe complex of the family arenaviridae and responsible for the argentinian, bolivian, and venezuelan hemorrhagic fever. the clinical manifestations of hemorrhagic fever are similar for the three viruses, mainly myalgia, arthralgia, headache, relative b ... | 1994 | 8021448 |
| the entry of junin virus into vero cells. | the entry mechanism of junin virus (jv) into vero cells was studied analyzing the effect of lysosomotropic compounds and acid ph on jv infection. ammonium chloride, amantadine, chlorpheniramine and procaine inhibited jv production. the action of ammonium chloride was exerted at early times of infection. virus internalization was inhibited and viral protein expression was not detected. when the extracellular medium was buffered at low ph, the ammonium chloride induced block on jv infection was ov ... | 1994 | 8031239 |
| calomys musculinus, the natural reservoir of argentine haemorrhagic fever, is a semipermissive host for a mouse-attenuated mutant of junin virus. | c167, a mouse-attenuated strain of junin virus derived from the xjc13 strain, also displayed reduced virulence for the south american cricetid calomys musculinus, a natural reservoir of this virus in nature. intracerebral inoculation of c. musculinus with 500 pfu of c167 produced only 25% mortality, whereas the parental xjc13 killed 85% of the animals. the attenuation of c167 for this cricetid was lower than for albino mice. the multiplication of c167 in c. musculinus-derived embryo or kidney fi ... | 1994 | 7839011 |