Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [ca antibodies (enterobacteriaceae common antigen) in the sera of domestic animals]. | using the indirect hemagglutination test, antibodies against enterobacteriaceae common antigen (ca) were tested in the sera of 123 horses, 142 cows, 108 sheep, 142 mature pigs and 60 piglets (3-4 weeks of age). anti ca antibody level and antibody titers for somatic antigens (phenol-water extracts) various serogroups of e. coli (0149, 0138, 0115, 078, 09) and s. typhimurium were compared. ca antibodies in titer equal or higher than 1:15 were found to occur in 100% of the examined horses and cows, ... | 1981 | 7029490 |
| chemotherapeutic activity of the combination of sulfachloropyridazine and trimethoprim against experimental colibacillosis of chickens and piglets and demonstration of the trimethoprim-induced potentiation of sulfachloropyridazine in vitro and in vivo. | 1981 | 7029707 | |
| escherichia coli resistant to tetracyclines and to other antibiotics in the faeces of u.k. chickens and pigs in 1980. | a survey conducted in 1980, 9 years after the banning of the use of tetracyclines as feed additives in the u.k., indicated that table chickens and pigs were still a large reservoir of tetracycline-resistant escherichia coli; the incidence of transferable tetracycline resistance was greater in chicken e. coli strains (68%) than in pig e. coli strains (20%). large amounts of sulphonamide-resistant and of furazolidone-resistant e. coli were found in the faeces of chickens; e. coli resistant to both ... | 1981 | 7031130 |
| intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes from bacterial and mammalian sources. | a simple method was developed for assessing the intramolecular coupling of active sites in the lipoate acetyltransferase (e2) component of the pyruvate dehydrogenase multienzyme complexes from escherichia coli, bacillus stearothermophilus and ox heart and pig heart mitochondria. samples of enzyme complex were prepared in which the pyruvate decarboxylase (e1) component was selectively and partly inhibited by treatment with increasing amounts of a transition-state analogue, thiamin thio-thiazolone ... | 1981 | 7032507 |
| effect of pectin on secretion in pig jejunal loops challenged to enteropathogenic e. coli or enterotoxin (lt). a preliminary report. | perorally administered pectin in pigs could reduce the fluid accumulation in intestinal loops challenged to different dilutions of enteropathogenic e. coli strains, no effect was observed to enterotoxin (lt) preparations. pectin seems to interact with the bacterial colonization. the neutralizing effect was most pronounced with low inoculation doses in the loops (10(3) and 10(5)), while high doses (10(9)) permitted the strains to exert their enteropathogenic effect (table i). different batches ga ... | 1981 | 7033924 |
| [model for in vivo study of antibiotics efficacy and level of escherichia coli implantation in the digestive tract (author's transl)]. | 1981 | 7044257 | |
| [histological and bacteriological examination of uterus from the repeat breeder gilt and sow (author's transl)]. | the investigation comprised sexual organs from 25 gilts and 97 sows culled because of repeat breeding. among the gilts 24.0 per cent and among the sows 25.8 per cent had endometritis. the following germs were present in uterus from gilts and sows with signs of endometritis: staphylococcus aureus, corynebacterium pyogenes, alpha-hemolytiske streptococcer, escherichia coli, pasteurella sp., aeromonas sp., acinetobacter sp. and citrobacter sp. among gilts and sows with germs present in uterus 40.9 ... | 1981 | 7322875 |
| k88-mediated binding of escherichia coli outer membrane fragments to porcine intestinal epithelial cell brush borders. | we have examined the interactions between various radiolabeled membrane fractions obtained from an enterotoxigenic escherichia coli strain and brush borders isolated from porcine intestinal epithelial cells. outer membrane fragments containing the k88 attachment factor bound tightly to brush borders, whereas cytoplasmic membrane vesicles did not. three different types of outer membrane preparations were tested: (i) cellular outer membranes isolated from lysozyme spheroplasts, (ii) medium vesicle ... | 1981 | 7012003 |
| internalization of 125i-insulin by im-9 cultured human lymphocytes: a comparison between a14-monoiodo-pork and biosynthetic human insulin. | human insulin synthesized from a- and b-chains separately produced in escherichia coli from cloned genes was characterized by examining its interaction biochemically and morphologically with im-9 cultured human lymphocytes. biosynthetic human insulin (bhi) behaves similarly to pork insulin with respect both to its binding properties and to the rate and magnitude at which it is internalized by the cells. | 1981 | 7011731 |
| comparison of the biologic activity of biosynthetic human insulin and natural pork insulin in juvenile-onset diabetic subjects assessed by the glucose controlled insulin infusion system. | to assess the biologic activity of biosynthetic human insulin (bhi) synthesized by escherichia coli, six insulin-dependent juvenile-onset diabetic subjects were studied with bhi and natural pork insulin, by means of the glucose controlled insulin infusion system (gciis). first, after an overnight normalization of blood glucose levels, the 24-h insulin requirement was determined while the patients were consuming a diet of 30 kcal/kg. then, the amount of glucose necessary to maintain normal blood ... | 1981 | 7011725 |
| dietary manipulation of gastric ph in the prophylaxis of enteric disease in weaned pigs: some field observations. | 1981 | 7039079 | |
| a method for the intraocular injection of micro-volumes in small animal species. | a method has been developed for the intraocular administration of small volumes (0.5-1.5 microliters) in small animal species such as guniea pigs and rats. the method was found to yield reproducible injection volumes and resulted in minimal ocular trauma. the aqueous humor of eyes injected with saline contained normal levels of protein and was free of white blood cells. responses to prostaglandin e2 and e.coli endotoxin were dose-dependent and as expected. | 1981 | 7049581 |
| effect of immunising pregnant sows with different escherichia coli vaccines on the antibody levels in the piglet sera. | six groups of pregnant sows were immunised with four different commercial escherichia coli vaccines. five groups were vaccinated parenterally while the other group was vaccinated orally and then parenterally. the colostral antibody titres to e coli o149 k91(b) k88 as measured by indirect haemagglutination were significantly higher in the vaccinated sows than in the controls. oral priming by vaccine feeding as opposed to parenteral administration did not appear to produce an anamnestic response. ... | 1981 | 7041409 |
| a survey of antibiotic resistance of escherichia coli isolated from farm animals in great britain from 1971 to 1977. | a total of 94,827 escherichia coli strains isolated from cattle, sheep, pigs and poultry during the years 1971 to 1977 were tested for resistance to six antibiotics using a disc diffusion technique. the percentage of strains resistant to chloramphenicol (10 microgram), streptomycin (10 microgram), tetracycline (10 microgram), neomycin (10 microgram) and furazolidone (15) were 16.8, 53.9, 42.7, 18 and 10.9 per cent, respectively; 52.2 per cent of strains (1971 and 1972) were resistant to 2 microg ... | 1981 | 7020230 |
| 4'-methylangelicin derivatives: a new group of highly photosensitizing monofunctional furocoumarins. | the photobiological properties of three new angelicin derivatives carrying a methyl-group in 4' position at the furanic ring have been studied. in double-irradiation experiments on e. coli cells, they appeared to behave as monofunctional reactives towards dna, similarly to the other angelicin derivatives previously studied. in short-term experiments such as the studies on macromolecular synthesis in ehrlich ascites cells, in which the assay is performed that after irradiation, before dna repair ... | 1981 | 7023974 |
| the isolation of antibiotic resistant coliforms from meat and sewage. | beef and pig carcases, meat products, frozen chickens, and sewage were examined in 3 separate surveys for antibiotic resistant coliforms. escherichia coli was isolated from 18 of 50 beef carcases; the numbers were low and resistance was found only to tetracycline. e. coli was isolated from 45 to 50 pig carcases; the numbers were high and showed a range of patterns of multiple antibiotic resistance. in meat products, the proportion of e. coli in contaminating organisms was low, and most resistanc ... | 1981 | 7016108 |
| [antibiotic resistance and transmission of r factors from e. coli and salmonella typhimurium, isolated from food samples of animal origin]. | 1981 | 7016497 | |
| [prophylaxis of colienterotoxemia in swine: duration of oral immunization with virulent pathogens combined with protective diet]. | 1981 | 7017925 | |
| a note on the use of the spiral plater to study the in vitro effects of amoxycillin, spectinomycin and chloramphenicol on a porcine escherichia coli. | 1981 | 6457824 | |
| direct and indirect effects of endotoxin on mitochondrial function. | 1981 | 6456464 | |
| determination of a specific receptor for formyl-methionyl-leucyl-phenylalanine on th pulmonary alveolar macrophage and its relationship to chemotaxis and superoxide production. | 3h-fmlp, a chemotactic peptide that resembles escherichia coli chemotactic factor, is chemotactic for pam, binds specifically to a site on the cell, and induces the generation of superoxide radicals by the cell. scatchard analysis revealed an equilibrium dissociation constant at 26 degrees c of 1.45 x 10(-8)m and the presence of 1.7 , 10(5) receptors per cell. binding was not inhibited by a partially purified c5a preparation or by the neutrophil-derived ccf but was inhibited by various n-formyla ... | 1981 | 6260878 |
| effect of escherichia coli heat-stable enterotoxin, cholera toxin and theophylline on ion transport in porcine colon. | 1. the effect of heat-stable enterotoxin (st) of escherichia coli, cholera toxin (ct), and theophylline (a phosphodiesterase inhibitor) on ion and water transport was studied with an in vivo isolated loop system of the pig colon.2. all three agents abolished net na absorption as a result of a decrease in the lumen to blood na flux alone. with all three agents, net cl absorption was reduced, but not abolished, and net hco(3) secretion was elicited. luminal p(co2) was reduced with ct and theophyll ... | 1981 | 6275079 |
| inactivation of microbial pyridoxal kinase by pyridoxal. | pyridoxal kinase from escherichia coli and bakers' yeast was inactivated by pyridoxal while the enzyme from rat and pig brain was not. the inactivation of the enzyme purified from e. coli was reversible and was rendered irreversible by the reduction with nabh4. this finding as well as a similar inactivation by 5'-deoxypyridoxal but not by 4'-deoxypyridoxine suggested that the inactivation was due to schiff base formation. the suggestion was confirmed by the incorporation of tritium label into th ... | 1981 | 6289642 |
| adhesion of enterotoxigenic escherichia coli in humans and animals. | enterotoxigenic escherichia coli (etec), an important cause of diarrhoea in humans and animal, require accessory virulence properties in addition to enterotoxin to manifest virulence. several classes of pili (hair-like protein surface organelles) promote adhesion of etec to small intestinal mucosa. antibody directed against adhesion pili interferes with colonization of the small intestine and prevents disease. this paper reviews studies with purified k88, k99 and 987 type pili used as parenteral ... | 1981 | 6114818 |
| pilus production, hemagglutination, and adhesion by porcine strains of enterotoxigenic escherichia coli lacking k88, k99, and 987p antigens. | three strains of enterotoxigenic escherichia coli which adhered, colonized intensively, and caused disease in pig intestine, but which did not produce pili of the k88, k99, or 987p antigen types were designated 3p(-) etec. the 3p(-) etec caused mannose-resistant hemagglutination, adhered to porcine intestinal epithelial cells in vitro, and produced pili. however, most bacteria taken directly from the intestine of pigs infected with 3p(-) etec appeared to be nonpiliated. two preparations were iso ... | 1982 | 6119295 |
| enteropathogenicity: recent developments. | recent studies have added important new information to our understanding of the pathogenesis and ethiology of diarrheal disease. vibrio cholerae produces a heat-labile enterotoxin, affecting cyclic amp. a very similar heat-labile enterotoxin is produced also by certain strains of escherichia coli, as well as by citrobacter, klebsiella, and aeromonas. e. coli may also produce a heat-stable enterotoxin, stimulating guanylate cyclase activity. in order to produce the pathologic effects, e. coli fir ... | 1982 | 6126611 |
| biological activities and spectroscopic properties of chromophoric and fluorescent analogs of adenine nucleoside and nucleotides, 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine derivatives. | the ribose-modified chromophoric and fluorescent analog of atp 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine 5'-triphosphate (tnp-atp) has been synthesized previously (hiratsuka, t., and uchida, k. (1973) biochim. biophys. acta 320, 635-647 and hiratsuka, t. (1976) biochim. biophys. acta 453, 293-297). in the present study, four tnp-derivatives of atp, adp, amp and adenosine were synthesized and compared for several chemical, spectral and enzymatic properties. their visible absorption a ... | 1982 | 6295507 |
| microbiological studies on hamburgers. | one hundred and eighty-two raw, 112 pre-cooked and 750 cooked hamburgers composed mainly of beef or beef and pork were subjected to microbiological examination.raw hamburgers gave total bacterial counts from 10(6) to 10(8) per g, counts of enterobacteriaceae from 10(4) to 10(6) per g, of escherichia coli from 10(3) to 10(5), of group d streptococci from 10(2) to 10(4), of staphylococcus aureus from 3 to 10(2) and of clostridium perfringens less than 10 bacteria per g. of the samples, 32% contain ... | 1982 | 6276464 |
| cloning, partial sequencing, and expression of glyceraldehyde-3-phosphate dehydrogenase gene in chick embryonic heart muscle cells. | two recombinant plasmids containing structural gene sequences of chick embryonic heart glyceraldehyde-3-phosphate dehydrogenase (gap dehydrogenase) were constructed and characterized. the plasmids pgap 30 and pgap 36 have inserts of 1200 and 950 base pairs, respectively. the identity of the clones was established by hybrid-arrested and hybrid-selection translation assays, and by immunoprecipitation of hybrid-selected translation product with gap dehydrogenase antiserum. hybridization of labeled ... | 1982 | 6179937 |
| immunological and physicochemical characterization of heat-labile enterotoxins isolated from two strains of escherichia coli. | heat-labile enterotoxins from escherichia coli strains of human and porcine origins had identical subunit composition, arrangement, and size in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. immunological differences recently described by others were shown to reside exclusively in the b subunits. | 1982 | 6183221 |
| [cloning and expression of the genetic determinants encoding for the serological variants of the adhesive factor k 88 carried by enterotoxinogenic escherichia coli strains of porcine origin]. | the existence of homologous sequences on plasmids carried by 3 enterotoxinogenic strains of escherichia coli of porcine origin, and encoding for adhesive factors of different antigenic specificities: k 88 ab, k 88 ac, k 88 ad; has been demonstrated by dna-dna hybridization. the use as probe of a dna fragment carrying a piece of structural genes of the adhesive factor k 88 ab taken out of a recombinant plasmid expressing this factor, has allowed us to localize the genetic determinants which encod ... | 1982 | 6186346 |
| protein loss in piglets infected with different enteropathogenic types of escherichia coli. | 1982 | 6749257 | |
| classification of trimethoprim-resistant dihydrofolate reductases mediated by r-plasmids using isoelectric focussing. | 1982 | 6749500 | |
| comparison of heat-labile enterotoxins from porcine and human strains of escherichia coli. | heat-labile enterotoxins (lts) from porcine ewd299) and human (h74-114) enterotoxigenic strains of escherichia coli were isolated by a single-step galactose elution procedure. although both strains had similar amounts of lt in their whole-cell lysates, h74-114 yielded a smaller quantity of purified lt than did ewd299. immunodiffusion studies with specific antisera revealed that although the two lts shared major antigenic determinants each also had unique antigens. both also had shared and unique ... | 1982 | 6176542 |
| evaluation of immunological methods for detection of bovine growth hormone (bgh) produced in e. coli. | the use of several immunological methods for studies on synthesis of bovine growth hormone (bgh) by e. coli is described here. the elisa procedure was shown to be the least sensitive and unfit for assaying bgh in e. coli extracts. the solid-phase radioimmunoassay (ria) proved to be highly sensitive, but since e. coli extract itself (not containing bgh) interfered with the immunological reaction, its use for measuring bgh was practically limited. the best adequate procedure proved to be radioimmu ... | 1982 | 6749998 |
| different pig phenotypes affect adherence of escherichia coli to jejunal brush borders by k88ab, k88ac, or k88ad antigen. | at least five different porcine phenotypes were distinguished with the three serological variants of the k88 antigen in the brush border adhesion test. pigs of one phenotype (a) are susceptible to adherence of all three variants, pigs of three phenotypes are susceptible to only two (b and c) or one (d) of the k88 variants, and pigs of one phenotype (e) are entirely resistant to adhesion of k88 antigen did not interfere with the adhesion of k88ab- or k88ac-positive escherichia coli, whereas in mo ... | 1982 | 6752028 |
| [harmless bacteria - harmful bacteria: unsolved problems of infectiology]. | 1982 | 6758178 | |
| small filter membrane bags for the study of antibiotic action in the digestive tract: bioavailability and in situ efficacy of ampicillin in the pig caecum. | bioavailability and activity of ampicillin were evaluated in the pig after preventive and curative doses by using small filter membrane bags containing strains of escherichia coli placed into the caecum. a peak concentration of 720 micrograms ampicillin per ml was obtained in caecal liquor which was effective against e coli k82 and e coli 127 in the bags, in pigs orally administered with 20 mg/kg of the drug. after intramuscular injection of ampicillin with 40 mg/kg, the peak concentration in ca ... | 1982 | 6760305 |
| [thymine-dependent mutants of swine-pathogenic edema e. coli of serotype 0139:82b]. | 1982 | 6762019 | |
| effects of epinephrine, clonidine, l-phenylephrine, and morphine on intestinal secretion mediated by escherichia coli heat-stable enterotoxin in pig jejunum. | perfusion of pig jejunum with escherichia coli heat-stable enterotoxin (strain 1261) reversed net absorption of water and electrolytes to net secretion. addition of the alpha-adrenergic agonists clonidine (5 x 10(-7) m) or l-phenylephrine (5 x 10(-6) m), or the opiate agonist morphine (3.6 x 10(-6) m) to the perfusate reduced the secretory response to enterotoxin and stimulated absorption in normal jejunum. epinephrine (5 x 10(-5) m) did not stimulate absorption in controls but reduced chloride ... | 1982 | 6762245 |
| bacterial adhesiveness and invasiveness in cell culture monolayer. 2. in vitro invasiveness of 45 strains belonging to the family enterobacteriaceae. | the invasive potential of 45 presumptive enteropathogenic and non-enteropathogenic bacterial strains belonging to the family enterobacteriaceae have been tested using the serény test and hep-2 cell monolayers examined by a combined light optical method. all the presumptive enteropathogenic strains of shigella dysenteriae, s. boydii, s. flexneri, s. sonnei and salmonella typhimurium showed in vitro invasiveness in the hep-2 cell culture test. fourteen presumptive non-enteropathogenic strains of e ... | 1982 | 6762798 |
| [usefulness of selected biological tests for detection of enteropathogenicity of escherichia coli strains isolated from children hospitalized for diarrhea]. | 1982 | 6820451 | |
| escherichia coli-associated porcine neonatal diarrhea: antibacterial activities of colostrum from genetically susceptible and resistant sows. | antibacterial properties of colostrum from genetically resistant and susceptible sows in a herd in which only the susceptible sows had acquired natural immunity to k88-positive escherichia coli have been investigated. significant differences in antiadhesive and opsonic activities occurred. colostrum from susceptible sows inhibited the binding of 125i-labeled k88 antigen to brush borders significantly better than did the colostrum from resistant dams. colostrum from susceptible dams effected more ... | 1982 | 7035361 |
| translation of poly(a) in eukaryotic cell-free systems. | translation of poly(a) in eukaryotic cell-free systems was investigated in comparison with that of poly(u), using the e. coli cell-free system as a control, and the following results were obtained. 1. poly(a) was not translated in the eukaryotic cell-free systems prepared from yeast, wheat embryos, and rat liver, whereas poly(u) was well translated in these systems. on the other hand, fairly good translation of poly(a) as well as poly(u) was observed in the e. coli cell-free system. 2. the ineff ... | 1982 | 7047515 |
| plasmid-mediated antimicrobial resistance in haemophilus pleuropneumoniae. | haemophilus pleuropneumoniae, isolated from the lungs of pig, was resistant to ampicillin, streptomycin, and sulfadiazine. this isolate, sd-1, possessed 1 plasmid (pvm105) coded for resistance to ampicillin and sulfadiazine, and the other (pvm104) for resistance to streptomycin and sulfadiazine. a 2nd isolate of h pleuropneumoniae, sd-2, recovered from a pig in a different outbreak of porcine pleuropneumonia, was resistant to streptomycin and sulfadiazine. these resistance determinants were on 1 ... | 1982 | 7046533 |
| [development of experimental keratoconjunctivitis as affected by the combined action of microbial agents and penicillin]. | 1982 | 7043982 | |
| rat preprorelaxin: complete amino acid sequence derived from cdna analysis. | 1982 | 7044231 | |
| molecular heterogeneity of heat-labile enterotoxins from human and porcine enterotoxigenic escherichia coli. | the heat-labile enterotoxins produced by human enterotoxigenic escherichia coli (lth) and porcine enterotoxigenic e. coli (ltp) were purified to homogeneity, and their molecular properties were compared with those of purified cholera enterotoxin (ct). on polyacrylamide gel disk electrophoresis without sodium dodecyl sulfate, lth, ltp, and ct differed in mobility, suggesting differences in their ionic charges. the pi values of lth, ltp, and ct were 7.50, 8.10, and 6.80, respectively. on sodium do ... | 1982 | 7141704 |
| virulence of escherichia coli in ascending urinary-tract infection in mice. | the virulence of escherichia coli strains in ascending urinary-tract infection was studied in mice drinking a 5% glucose solution; factors determining the virulence were examined. of 33 strains, 8 (group i) infected the bladder and kidney, 10 (group ii) infected only the bladder, while the remaining 15 strains (group iii) did not cause infection. the adherence of group-i and group-ii strains to bladder epithelial cells in vitro was inhibited by d-mannose. in group iii, 13 strains barely adhered ... | 1982 | 6126593 |
| appearance of enterotoxigenic escherichia coli in piglets with diarrhea in connection with feed changes. | twelve weaned piglet (3-week-old) were divided into three groups according to time of feed change and observed for diarrhea during the time they were 3 to 8 weeks of age. a total of 553 strains of escherichia coli were isolated from rectal fecal samples and examined for heat-labile (lt) and heat-stable (st) enterotoxins, pilus antigens (k88, k99, and 987p), hemolysin (hly), raffinose utilization (raf) and drug resistance. enterotoxins and/or hemolytic e. coli strains appeared in the rectal feces ... | 1982 | 6752665 |
| [alpha-neoendorphin]. | 1982 | 6754998 | |
| [secretin production by genetic engineering]. | 1982 | 6754999 | |
| clindamycin: a review of fifteen years of experience. | clindamycin, the 7(s)-chloro-7-deoxy derivative of lincomycin, has stood the test of time in the treatment of anaerobic infections. clindamycin inhibits protein synthesis by acting on the 50s ribosomal subunits of bacteria. the colitis resulting from the use of clindamycin has been extensively studied and is now easily manageable. although newer antibiotics active against anaerobes are available, clindamycin remains a reliable and well-tested antibiotic for use in anaerobic infections. | 1982 | 6818656 |
| the biotyping of escherichia coli isolated from healthy farm animals. | a total of 2973 escherichia coli, isolated from six different groups of animals, were examined for their ability to ferment adonitol, dulcitol, raffinose, rhamnose and sorbose in solid media. twenty-nine fermentation patterns were recorded although 2443 (82%) of the e. coli belonged to seven of the 32 possible biotypes. ninety-six o-serotypes were identified within the 2973 e. coli. the number of o-serotypes represented in the 15 most common biotypes ranged from three to 15. serotypes o8 and o9 ... | 1982 | 7045219 |
| [production and use of hyperimmune heterologous horse serum against colibacteriosis in swine]. | 1982 | 7051528 | |
| [posttraumatic osteomyelitis. mode of infection, significance of metal implants]. | guniea-pigs of the duncan-hartley strain were used in a model of posttraumatic osteomyelitis. one femur had been fractured with or without consequent metal implantation and infected subsequently with 10(7) or 10(5) staphylococcus aureus or e. coli. 85% of the animals infected with staph. aureus and 56% infected with e. coli developed osteomyelitis. healing of infection occurred in 3 cases only and always in the absence of metal implants. all bone infection persisting for more than 3 months were ... | 1982 | 7047331 |
| protection against colibacillosis in neonatal piglets by immunization of dams with procholeragenoid. | protection against colibacillosis in neonatal piglets was obtained by immunization of pregnant dams with procholeragenoid. procholeragenoid is a stable high-molecular-weight aggregate of cholera toxin formed during the heating of cholera toxin. procholeragenoid retained approximately 1% of the toxicity of native toxin as determined in the rabbit ileal loop and y-1 adrenal cell assays and 5% of the activity in the rabbit skin assay. immunization of pregnant dams with 50 micrograms of procholerage ... | 1982 | 7040250 |
| gm1 ganglioside enzyme-linked immunosorbent assay for detection of heat-labile enterotoxin produced by human and porcine escherichia coli strains. | human and porcine enterotoxigenic strains of escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the gm1 ganglioside enzyme-linked immunosorbent assay (gm1-elisa) and the y1 adrenal cell assay. when testing for enterotoxigenicity by the gm1-elisa technique, homologous antisera for human and porcine heat-labile enterotoxins had to be used to detect enterotoxigenic strains of human and porcine origi ... | 1982 | 7040450 |
| pulmonary dysfunction in sepsis: is pulmonary edema the culprit? | pulmonary dysfunction is a well-recognized sequela of sepsis, which has been quantitated by calculation of intrapulmonary shunt (qs/qt) and, more recently, by measurement of extravascular lung water (evlw). we sought to demonstrate the relationship between qs/qt and evlw in sepsis. nine pigs were given live e. coli infusions and five control pigs received only crystalloid. pulmonary artery pressure (pap), pulmonary artery wedge pressure (pawp), arterial blood gases (abg), and mixed venous blood ... | 1982 | 7042992 |
| porcine neonatal coccidiosis in quebec. | intestinal coccidiosis was diagnosed in 110 diarrheic piglets originating from 66 farrowing operations. these piglets had been submitted alive for necropsy early after the beginning of diarrhea, and the diagnosis of coccidiosis was based on the demonstration of coccidial forms in their jejunum by histopathological examination. enteropathogenic strains of escherichia coli were demonstrated in only nine of the 110 piglets while the transmissible gastroenteritis virus and rotavirus were each presen ... | 1982 | 17422160 |
| oral immunization of sows: anti-k88 antibodies in serum and milk of the sow and in serum of the piglets. | pregnant sows were immunized by oral application of live e. coli. the effect of immunization was demonstrated by measuring the titers of anti-k88 antibodies in sow serum, colostrum and milk as well as in piglet sera using enzyme-linked immunosorbent assays. the isotype involved in anti-k88 reactivity was found to be iga. by comparing iga-titers in colostrum and milk, the local production of this ig-class in the mammary gland is suggested. | 1982 | 15861583 |
| dietary regimen, rotavirus, and hemolytic enteropathogenic escherichia coli in weanling diarrhea of pigs. | previously, we induced weanling diarrhea in piglets by infecting them first with rotavirus followed by a hemolytic enteropathogenic escherichia coli. we postulated that rotavirus, by damaging the epithelium of the small intestines, produced an entero-environment which favored the selection and growth of the enteropathogenic e. coli. furthermore, diet might affect the entero-environment and influence the course of the disease. to test this, newly weaned 3-week-old piglets were assigned to one of ... | 1983 | 6375527 |
| gastric mucosal blood flow and escherichia coli bacteremia. | the specific changes in gastric blood flow during sepsis are controversial. previous investigations of intragastric blood flow using endotoxin models revealed decreased total gastric blood flow and decreased gastric mucosal blood flow. endotoxin models are now thought to be inadequate due to the accompanying depression of the systemic circulation. more recently, increased gastric blood flow has been demonstrated in a septic hindlimb model. to further elucidate the changes in intragastric blood f ... | 1983 | 6340545 |
| drug resistance and conjugative r plasmids in fecal escherichia coli strains isolated from healthy younger animals (chickens, piglets, calves) and children. | a total of 11,777 escherichia coli strains were isolated from 90 chickens, 103 piglets, 96 calves, and 104 children in 1979 in gunma prefecture and tested for drug resistance and the presence of conjugative r plasmids. the percentages of individuals that excreted drug-resistant strains were: chickens, 100%; piglets, 99%; calves, 100%; and children, 64%. the frequency of isolation of drug-resistant strains among the total isolates was: chickens, 98%; piglets, 93%; calves, 94%; and children, 41%. ... | 1983 | 6374389 |
| the clinical manifestation and pathogenesis of enteritis associated with rotavirus and enterotoxigenic escherichia coli infections in domestic animals. | rotavirus and enterotoxigenic escherichia coli (etec) are enteropathogens each capable of inducing diarrhoea in some animal species and man. unstressed young animals develop an age-related resistance to infection with either rotavirus or etec which differs for each animal species. the effects of experimental infection of calves, lambs, foals and piglets with rotavirus and etec given either alone or in combination, have been examined. in general, dual infections tended to lengthen the period of a ... | 1983 | 6361857 |
| quaternary heterocyclylamino beta-lactams. v. l-640,876 treatment of induced enterotoxigenic colibacillosis (scours) in calves and piglets. | a new semisynthetic cephalosporin antibiotic designated l-640,876, 7-beta-(1-benzylpyridinium-4-yl)amino-3-[( (1-methyl-1h-tetrazol-5-yl)thio] methyl)ceph-3-em-4-carboxylate, was highly active in vitro against 110 enteropathogenic strains of escherichia coli and salmonella species of animal origin. the mic90 was 0.125 microgram/ml for the e. coli strains, 2 micrograms/ml for the s. choleraesuis strains and 4 micrograms/ml for the s. typhimurium strains. in colostrum-fed calves infected with e. c ... | 1983 | 6381448 |
| [passive immunoprophylaxis against coli dysentery of suckling pigs by vaccination of sows with attenuated escherichia coli mutants. 1. clinical and laboratory diagnostic results of the combined use of k88- and k99-antigen-containing strains derived from escherichia coli mutants]. | 1983 | 6362603 | |
| from the national institute of allergy and infectious diseases. summary of a workshop on enteropathogenic escherichia coli. | 1983 | 6343525 | |
| drug resistance and r plasmids in escherichia coli isolated from laboratory animals. | 1983 | 6345875 | |
| antisecretory effects of berberine with morphine, clonidine, l-phenylephrine, yohimbine or neostigmine in pig jejunum. | the effects of berberine alone or in combination with morphine, clonidine, l-phenylephrine or yohimbine were compared in escherichia coli heat-stable enterotoxin (st)-exposed ligated jejunal loops in 2 week old pigs. in addition, net water and electrolyte fluxes in normal jejunal loops were measured in the presence of neostigmine, morphine, clonidine, l-phenylephrine or yohimbine alone or in combination with berberine. berberine, morphine, clonidine and l-phenylephrine each reduced the net secre ... | 1983 | 6363101 |
| acid-base and electrolyte changes in 1-3 days old piglets infected with enteropathogenic escherichia coli and in spontaneous cases of piglet diarrhoea. | 1983 | 6346838 | |
| receptor analogues and anti-pili antibodies as inhibitors of bacterial attachment in vivo and in vitro. | 1983 | 6135384 | |
| multi-adhesin vaccines for the protection of the neonatal piglet against "e. coli" infections. | it has been shown that the initial stages of enteric infection with e. coli organisms involve attachment of the organism to the intestinal villi. attachment is mediated by specific protein adhesins on the surface of the organism such as k88ab, k88ac, k99 and 987p (1). purified preparations of each of these adhesins have been used for the vaccination of pregnant gilts and been shown to confer passive protection to their piglets against homologous but not heterologous challenge with e. coli (2, 3) ... | 1983 | 6135633 |
| the production of f41 fimbriae by piglet strains of enterotoxigenic escherichia coli that lack k88, k99 and 987p fimbriae. | the enterotoxigenic escherichia coli strains 1676, 1706, 1751 and kec96a, which do not produce fimbrial adhesive antigens of the k88, k99 or 987p antigen type reacted both in vitro and in vivo with antiserum to f41 fimbriae in an indirect immunofluorescent antibody technique. antiserum used to demonstrate material b, an adhesive antigen thought to mediate the adhesive and mannose-resistant (mr) haemagglutinating properties of e. coli strains 1676, 1706 and 1751, reacted in vitro with an f41+ str ... | 1983 | 6138394 |
| density and localization of p-fimbriae-specific receptors on mammalian cells: fluorescence-activated cell analysis. | more than 95% of acute non-obstructive pyelonephritis cases in children are caused by p-fimbriated escherichia coli. uroepithelial cells from individuals prone to urinary tract infections bind such bacteria more avidly than do cells from healthy controls. a high density of receptors for p-fimbriae on cells of the urinary tract and kidney may thus be an important host factor in the acquisition of upper urinary tract infections. in this communication we are describing methods to determine the dens ... | 1983 | 6132882 |
| aspects on structure and function of pili on uropathogenic escherichia coli. | 1983 | 6131425 | |
| heme-iron and ecology of escherichia coli within the porcine gut. | growth of escherichia coli was followed in sow whey by a turbidometric technique. heme-iron clearly promoted bacterial growth, therefore free heme would be dangerous in small intestine by enhancing bacterial growth: duodenal brush border membrane (bbm) was seen to bind heme-iron, thus abolishing the growth promoting effect of this form of iron. scatchard analysis of heme-binding onto bbm showed that heme became bound by a specific mechanism (kd approximately equal to 10(-7) m) as well as non-spe ... | 1983 | 6368116 |
| [dynamics of antibiotic resistance of strains of escherichia coli isolated from feces of weaned piglets (the case of the lwanika- lubumbashi (zaire) farm]. | 1983 | 6369447 | |
| study of several factors affecting the agglutinating activity of k99-positive escherichia coli strains. | the effect of several factors on escherichia coli k99-plasmid associated agglutination has been studied. the results obtained indicate that escherichia coli 637 (k99+) mediated red blood cell agglutination is unspecific although the agglutination titres for several erythrocyte species are significantly different. the agglutination is highly stable (at least with sheep red blood cells) to changes in temperature (from 4 degrees c to 37 degrees c), to changes in ph (from 5 to 9) and to the presence ... | 1983 | 6371953 |
| the role of dietary antigen in the aetiology of post weaning diarrhoea. | experiments were undertaken to investigate whether a hypersensitivity response to dietary antigen might be a predisposing factor in the aetiology of post weaning diarrhoea. the results indicated that: 1. very small amounts of food given to baby pigs before weaning significantly increased the severity and accelerated the onset of the disease; 2. post weaning diarrhoea was dependent upon the presence within the diet of antigenic material; 3. weaning diets containing little antigenic material had l ... | 1983 | 6375529 |
| vaccination of piglets against post-weaning "e. coli" enterotoxicosis. | e. coli enterotoxicosis is one of the main causes of neonatal diarrhea in piglets. for their protection against this disease a vaccine has been prepared based on k88 and lt. pregnant sows were vaccinated, thereby transferring passive immunity to their offspring through colostrum and milk. protection was obtained in terms of decrease in mortality, occurrence of diarrhea and excretion of enteropathogenic bacteria. piglets from non-immune sows could be immunized shortly after birth. however, piglet ... | 1983 | 6347759 |
| plasmid-mediated adhesion in enteropathogenic escherichia coli. | a survey of classical serotype enteropathogenic escherichia coli has been made with respect to their plasmid profile and ability to adhere to hep-2 cells. thirty-one of the 32 strains examined contained a 50-70 md plasmid, and many exhibited hep-2 adherence. strain e2348 (0127:h6), which causes diarrhea in volunteers and is hep-2-adhesive, was chosen for further study. the large 55 md plasmid in e2348, pmar2, has been marked with a transposon coding for ampicillin resistance. e2348 that has been ... | 1983 | 6352891 |
| epizootiological studies on r plasmid with carbadox resistance. | 1983 | 6355590 | |
| differential toxicity of inhaled gram-negative bacteria. | the toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. these microorganisms were not equally toxic. citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglobin 24 to 48 h after inhalation. other strains tested failed to provoke such statistically consistent increases. measurements of c-reactive protein in these same animals did not lead ... | 1983 | 6339400 |
| an in vitro system to study interactions between bacteria and epithelial cells at the molecular level. | this paper describes an experimental system to study interactions between porcine enterotoxigenic escherichia coli (etec) and porcine intestinal epithelial cells in vitro at the molecular level. radiolabelled bacteria or bacterial membrane fractions were incubated with brush borders prepared from purified epithelial cells, which were then washed repeatedly. the bacterial components removed by washing or retained by the brush borders were analysed to determine their composition and source. for th ... | 1983 | 6339675 |
| partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. | a factor produced by several strains of escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on cho, vero, and hela cells. the same strains were found to have hemolytic activity on sheep erythrocytes. the toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from vero toxin. the main effect induced by the isolated factor on ... | 1983 | 6341235 |
| intraspecific interactions between escherichia coli strains in human newborns and in gnotobiotic mice and piglets. | a plasmid-free human escherichia coli strain emo, was inoculated to human newborns within two hours of life. it became established in the feces at a high population level. ampicillin and tetracycline resistant e. coli strains disappeared or decreased to subdominant level in the inoculated newborns whereas they remained at a high level in the noninoculated newborns. strain emo was also shown to exert a barrier effect against a porcine plasmid-bearing enterotoxigenic ent+ k88- e. coli strain in gn ... | 1983 | 6361853 |
| the effect of antibiotics on mannose-resistant haemagglutination by k88- and k99-positive escherichia coli strains. | five e. coli strains carrying k99 antigen isolated from the intestines of calves which had succumbed to diarrhoea and six k88-positive strains isolated from fatal cases of diarrhoea in piglets were examined for their mannose-resistant haemagglutination (mrha) capacity against pig erythrocytes. the bovine strains showed a geometric mean mrha-titre of 1/18 and the porcine strains one of 1/45. similar experiments were carried out after addition of the following antibiotics in doubling dilutions: am ... | 1983 | 6355504 |
| enterotoxigenicity of bovine and porcine escherichia coli of o groups 8, 9, 20, 64, 101, and x46. | 1983 | 6349437 | |
| [pharmacological, toxicological, microbiological and pathohistological studies of gentamycin complex ii]. | the acute toxicity was determined of the gentamycin complex ii (gmc-ii) for albino mice, containing gentamycin components in a varying ratio. the ld50 at intraperitoneal application was 347.7 mg/kg body mass, while at oral application it was 5,180 mg (4933 divided by 5439) mg/kg body mass. histopathologically, (mice killed with high doses of gmc-ii, given orally) there were diffuse hyperemia of the liver, parenchymal degeneration of the kidney tubuli epithelium, and edema and emphysema of the lu ... | 1983 | 6623911 |
| [clinical, cytological and microbiological studies of the mma syndrome]. | clinical, cytological, and microbiological investigations were carried out of a total of 793 sows in the course of 10 days after farrowing. it was found that the mma syndrome was manifested in the first days following farrowing with higher temperature, changes in the genital organs with a purulent discharge from the first to the third day, and lesions in the mammary gland with inflammatory processes or decrease in the milk secretion. the cell content of milk of the diseased sows was higher. the ... | 1983 | 6659347 |
| safety evaluation of thaumatin (talin protein). | thaumatin, the sweet proteinaceous extract of the arils of thaumatococcus daniellii (benth.) has been studied for its subacute toxicity in rats and dogs and its ability to produce anaphylactic antibodies following oral administration to rats and normal human subjects. thaumatin was readily digested prior to absorption in rats and no adverse effects resulted from its continuous administration to rats and dogs at dietary concentrations of 0, 0.3, 1.0 and 3.0% for 13 wk. it was not teratogenic when ... | 1983 | 6686588 |
| novel mucosal anti-microbial functions interfering with the plasmid-mediated virulence determinants of adherence and drug resistance. | mucosal antibodies in vivo and in vitro interfere with the stability of plasmids coding for important virulence determinants in porcine enteropathogenic e. coli (eec), such as the adhesion determinants k88ab and k88ac. the effector antibody is not directed against k88 antigens and is not serotype specific, but an antigen common to k88+ strains is implicated. further lack of pathogen specificity is exemplified by antibody elimination of the more recently discovered k88ad plasmid. antibodies that ... | 1983 | 6191616 |
| immunopathogenesis of chronic pyelonephritis. | immunopathologic responses to urinary tamm-horsfall protein in the development of chronic pyelonephritis were examined by four different approaches. first, in a rabbit model, tubulointerstitial nephritis developed in 64 of 102 rabbits injected intravenously with urine or rabbit tamm-horsfall protein as compared with only one of 17 rabbits in two control groups. circulating cytotoxic lymphocytes plus immunoglobulin g (igg) antibodies against tamm-horsfall protein were found in 51 percent of chall ... | 1983 | 6192715 |
| use of immunofluorescence, gram's staining, histologic examination, and seroagglutination in the diagnosis of porcine colibacillosis. | an indirect fluorescent antibody (ifa) test of ileal impression smears for k88, k99, and 987p pilus antigens was compared with histologic examination and seroagglutination of escherichia coli isolates for efficacy in determining colibacillosis in pigs. histologic examination appeared to be more effective than the ifa test in revealing colonization of the ileum by bacteria. however, histologic examination revealed little about the nature of the colonizing bacteria. correlation between bacterial a ... | 1983 | 6195944 |
| role of the capsule and the o antigen in resistance of o18:k1 escherichia coli to complement-mediated killing. | epidemiological data show that o18:k1 escherichia coli is a common cause of neonatal bacteremia and meningitis. these bacteria were capable of multiplying in the bloodstream of newborn rats and were resistant to the bactericidal effects of complement in the absence of specific antibodies. the roles played by the o antigen and the k antigen in complement resistance were analyzed by comparing the bactericidal effects of normal sera and of sera deficient in various complement components or in immun ... | 1983 | 6196296 |
| o-antigens, k-antigens and production of enterotoxin in escherichia coli-strains isolated from litter mate piglets with neonatal diarrhea. | 1983 | 6197871 | |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| bacterial cell wall components decrease the number of guinea-pig lung beta-adrenoceptors. | infections of the deeper respiratory airways can contribute to the progression of chronic asthmatic bronchitis. in the present report a number of microorganisms affecting the number of beta-adrenoceptors in guinea-pig lung homogenates are described. haemophilus influenzae, streptococcus pneumoniae, bordetella pertussis and escherichia coli o111b4 induced a significant decrease of the number of beta-adrenoceptors (by approximately 20%). staphylococcus aureus, influenza a virus and escherichia col ... | 1983 | 6301848 |
| relatedness of chloramphenicol resistance plasmids in epidemiologically unrelated strains of pathogenic escherichia coli from man and animals. | i have examined 20 plasmids conferring chloramphenicol resistance (cm) in multiresistant strains of escherichia coli pathogenic for man and piglets. in denmark, one plasmid family, exemplar phg33, is responsible for all chloramphenicol resistance in serotypes of e. coli found in diseased piglets. a closely related plasmid, phg50, was identified in an enteropathogenic e. coli (epec) strain from an infant. the isolate was epidemiologically unrelated to the piglet isolates. the molecular relatednes ... | 1983 | 6302257 |